KR20090102378A - Natural germicide for mugwort and method of manufacturing thereof - Google Patents

Abstract

PURPOSE: A natural bactericidal disinfectant and a method for manufacturing the same are provided to perform eco-friendly organic farming without damage to the crops. CONSTITUTION: A method for manufacturing a natural bactericidal disinfectant comprises: a mixing 60-85 % of Artemisia princeps var. orientalis, 4-10 % of garlic, 4-10 % of red pepper, and 7-20 % of charcoal in 100 L of purified water; a step of heating over 100°C to obtain 70 L of composition; a step of filtering solid ingredients; a step of putting extract composition in culture room for microorganism; a step of adding microorganism of 4 kinds is of liquid microorganism; a step of mixing with 1kg of glucose and 2 kg of molasses and culturing for three days; and a step of adding liquid microorganism and mixing.

Description

Natural germicide disinfectant with mugwort as main ingredient and its manufacturing method {Natural germicide for mugwort and method of manufacturing thereof}

The present invention relates to a natural disinfectant disinfectant using the mugwort as a main raw material and a method for producing the same, and more specifically, prepared by mixing and incubating liquid microorganisms in an extract composition obtained by putting mugwort, garlic, red pepper, and charcoal in water. Diluted disinfectant disinfectant to hundreds or thousands of times with water can suppress ammonia gas generation and drastically reduce livestock waste water, greatly improving the surrounding environment, and manure decomposed by microorganisms can be immediately composted It is a natural disinfectant disinfectant that can not only realize organic farming, but also replace pesticides when spraying on crops, and suppresses intestinal pathogens of livestock to reduce mortality, increase weight gain, and shorten the delivery period, thereby contributing to the income increase of farmers and livestock farmers. It is about.

Today, the breeding aspect of the livestock sector is transforming into a large-scale, dense breeding aspect, and accordingly, the speed of disease transmission and the scale of damage are inevitably increased.

In particular, the environment inside and outside the barn is contaminated with various organic substances (pollutants) and pathogens (viruses, bacteria, molds) that are already remaining, and the risk of disease development is high. In order to minimize the pollution of the surrounding environment inside and outside the livestock's livestock farms, livestock farms are not only cleaned but also halogen-based (chlorine and iodine), phenolic (cresol and synthetic phenols) and quaternary ammonium. However, these contaminants provide a hidden place for pathogens because of their large size when compared to particles of viruses, bacterial fungi or disinfectants, giving them the opportunity to survive.

In addition, the disinfectant is absorbed to lower the effective concentration of the disinfectant, and the pH of the surrounding environment is also changed to invalidate the disinfectant. This resulted in a bad effect on the effectiveness of the disinfectant. In addition, the manure of untreated livestock was bad smell due to the generation of harmful gases, and crops were ill due to the use of excessive gas disinfectant, which could not be used as fertilizer.

In addition, E. coli, Staphylococcus aureus, and Salmonella, one of the causes of the disease, have specificity that can be settled in the intestinal tract, and studies on vaccines and infectious diseases have been carried out, but there are many types of bacteria, which limit the prevention of vaccines.

Therefore, excessive chemical disinfectant spraying inside and outside the barn is known as a major cause of environmental and soil pollution. Therefore, improvement of the environment around the barn is urgently needed more than ever, and the effect of suppressing intestinal bacteria, which is a risk factor of disease occurrence, is weak. It was.

 The present invention has been made as a result of repeated research to improve the surrounding environment due to excessive chemical disinfectant spraying, the main purpose is to reduce the surrounding environment and water pollution by suppressing the generation of ammonia gas generated during fermentation of livestock manure It is to provide a natural disinfectant disinfectant.

 Another objective is to provide natural disinfectant disinfectants that can minimize livestock excretion and raise livestock in a clean environment to prevent disease and increase income for livestock farmers.

Another purpose is to realize eco-friendly organic farming without harm to crops even if it is used as a fertilizer for environmentally treating manure excreted by livestock, and can replace pesticides when diluting with water and spraying crops. It is to provide natural disinfectant disinfectants that can contribute to the increase of income.

Another object is to provide a natural disinfectant disinfectant that can suppress the intestinal pathogens of livestock, reduce mortality, increase the growth rate, and shorten the shipping period and contribute to the increase of farm income.

In order to achieve the above object, the present invention is 10) 10 kg of ingredients mixed in 100 l of purified water by weight ratio of mugwort 60 ~ 85%, garlic 4 ~ 10%, red pepper 4 ~ 10%, charcoal 7 ~ 20% To put into 100 ℃ or more to obtain a step of 70L composition; Ii) Filtering the extract with a fine filter net, and then put the extracted composition into a microorganism incubator Lactobacillus sp. Four kinds of liquid microorganisms including 10 l, Aspergillua niger 5 l, Bacillus subtillis 5 l, and Saccharomyces cerevisiae 5 l are added to the mixture and mixed with 1 kg of glucose and 2 kg of molasses for 3 days at 25 ° C until pH 2 ~ 3. Culturing the above; Iv) Nitrosomonas 5 L of the liquid microorganisms of the culture composition is added to the extraction composition is secondary, and refrigerated to maintain 3 ~ 5 ℃ to mix; Provided is a method for producing a natural disinfectant disinfectant.

In addition, in order to achieve the above object, to provide a natural disinfectant disinfectant completed by the manufacturing method.

In addition, in order to achieve the above object, a natural sterilizing disinfectant is diluted with 100 times the water and the natural sterilization disinfectant completed by the manufacturing method to provide a livestock disinfectant disinfectant to spray on the bottom of the barn.

In addition, in order to achieve the above object, it provides a natural disinfectant disinfectant for crops to be sprayed on the crops by diluting the natural disinfectant disinfectant with 1000 times the water prepared in the manufacturing method.

In addition, in order to achieve the above object, to provide a natural disinfectant disinfectant for livestock to supply the drinking water to the livestock by diluting the natural disinfectant disinfectant with 300 times the water completed in the manufacturing method.

As a result of diluting the natural disinfectant disinfectant prepared by the present invention with water and spraying it on the bottom of the barn, the experimental group had better antibacterial effect than the control group, and almost no test manure discharge occurred, and caused carbon dioxide, ammonia, Hydrogen sulfide was also significantly lower, the number of breeding days was significantly reduced, and respiratory disease and diarrhea did not occur. In addition, it can reduce the mortality rate, increase the growth rate, and shorten the delivery period by suppressing the intestinal pathogens of livestock, thereby contributing to the increase of farm income. The difference is that the production of various alcohols produced during fermentation of organic matter by the added beneficial microorganisms accelerated the evaporation of water. In particular, organic acids produced by fermented microorganisms suppressed the growth of decaying microorganisms contaminated with feces. While suppressing the production, it exhibits the effect of maintaining the growth of microorganisms that ferment organic matters, thereby suppressing the disease that occurs frequently in breeding, and the concentration of ammonia, which occurs most when decomposition of livestock manure, is almost Not only did it increase the odor suppression effect, but the decomposed manure could be composted immediately, thus realizing eco-friendly organic farming.

The present inventors have developed a natural disinfectant disinfectant using wormwood in order to solve the problems of the conventional barn, and sprayed on the bottom of the barn, the harmful gas generated during the rapid disappearance of livestock feces and decomposition of feces, namely ammonia, Environment-friendly farming that contributes to increasing farm income by minimizing the generation of hydrogen sulfide, carbon monoxide, carbon dioxide, and carbon dioxide, and improving the environment in the breeding area, and suppressing the growth of bacteria in the intestines when feeding livestock, resulting in low disease incidence and high growth rate. To achieve the purpose by realizing the bar, the ingredients and efficacy of the raw materials mainly used here are as follows.

Mugwort contains medicinal ingredients such as cineol, choline, adenine, monoginine, eucalyptol, and artemisine. It is warm, non-toxic and tastes bitter.

Chinenol (chneol) is a natural organic compound, which is used for the treatment of chronic obstructive pulmonary disease and asthma, can prevent respiratory diseases caused by turbid air in the barn. In addition, anti-inflammatory action has a bactericidal effect, and improves immunity.

Choline (choline) is one of the vitamin B complex has the effect of preventing fatty liver (脂肪 肝). In the process of supplying excessive nutrients to raise livestock and administering excessive antibiotics, the liver's liver cannot detoxify, causing stress, death, contraction, and deterioration.

Adenine (Adenine) has a basic, nucleic acid ADP, FMN, NAD, NADP, COA, etc. reacts in vivo in the livestock to prevent disease and have immunity.

In addition, Monogine has detoxification and hemostatic effects, Eucalyppol has a unique aroma and refreshing taste, and Artemisine is a natural substance, not a chemical antibiotic, which kills malaria. It was found that the antibacterial activity is strong. In particular, it is known to have effects such as strong blood clotting, detoxification, antibacterial, tonic, gangjeong, anti-inflammatory, analgesic, immunity, diuresis, hemostasis, and appetite (improved feed efficiency) of the human body and animals. The fragrance is effective in killing or inhibiting proliferation of Staphylococcus aureus, hemolytic streptococci, Escherichia coli, diphtheria bacteria, and malaria bacteria.

Red pepper contains protein, lipid, sugar, fiber, ash, calcium, phosphorus, iron, sodium, potassium, retinol, carotene, vitamin A1 (IU), vitamin B1 (IU), vitamin B2, vitamin C, niacin, etc. There are base components such as adenine, betaine, choline, capsanthine, beta-carotene, and lutein. It also contains a large amount of capsaicin, which prevents rancidity and helps the growth of lactic acid bacteria. Red pepper promotes secretion of gastric juice, promotes food consumption, promotes blood circulation, and dilutes mucus to easily drain phlegm out of the body. In addition, the capsaicin component is effective to sterilize.

Garlic contains ingredients such as capsaicin, allicin, and organic sulfur. Capsaicin is a colorless volatile organic compound with a pungent taste, allicin has a strong antibacterial action, and organic sulfur is changed to alysin with a pungent taste and smell by the enzyme of alinase. Everyone knows that garlic is good for the human body. Recently, research on garlic is being actively conducted in developed countries such as Europe. Garlic juice does not grow cholera, vibrio, staphylococcus, and fungus even in a 120,000-fold dilution. Typhoid bacteria die in five minutes and have a stronger bactericidal activity than antibiotics such as penicillin and terramycin.

Charcoal contains five major minerals such as calcium, potassium, iron, phosphorus and sodium, and contains copper, zinc, manganese, magnesium and cron. The wood contains 0.5 to 1% of minerals, which is about 5 times as charcoal. Charcoal cleanses, detoxifies, filters, deodorizes and emits negative ions, giving a sense of stability to brain waves, radiating far-infrared rays to help blood circulation, and cations in the air combine with genes of char to create air of negative ions and give a refreshing feeling. .

Glucose is one of the monosaccharides. White crystals with a sweet taste, soluble in water, and shriveled. It is widely distributed in the biological system and is broken down through cell respiration to produce energy, which is stored in the form of ATP. This energy is used for fermentation and breathing.

Molasses is a by-product of making sucrose, which includes waste molasses that is not edible in a broad sense. However, in a narrow sense, it refers to the honey that is produced when the crude sugar is refined or when the ice sugar is made. Molasses is a pale yellow, transparent, viscous sugar solution. The molasses used in agriculture is a liquid substance with a high viscosity with a sugar content of 50-60% and a specific gravity of about 1.39. It is mainly used as a carbon source in fermentation of various raw materials.

Microorganisms used in the present invention are Lactobacillus sp., Bacillus subtilis, Aspergillus niger, Saccharomyces cerevisiae It contains all Nitrosomonas bacteria, and these diluted five microorganisms are mixed with the above-mentioned raw material extract and incubated at a predetermined temperature, which is a natural disinfectant disinfectant that can dramatically improve the environment of the house. Can be prepared.

Here, the genus Nitrosomonas has the effect of decomposing ammonia gas into nitrogen gas, and the genus Lactobacillus and Saccharomyces cerevisiae are alcohols produced during fermentation, which inhibits bacterial penetration and protects against diseases. Have

In the present invention, 10) 100 kg of purified water, mugwort 60 ~ 85%, garlic 4 ~ 10%, red pepper 4 ~ 10%, 7 ~ 20% of charcoal mixed in a weight ratio of 10 kg of the material and heated to 100 ℃ or more Obtaining 70 liters of the composition; Ii) Filtering the extract with a fine filter net, and then put the extracted composition into a microorganism incubator Lactobacillus sp. Four kinds of liquid microorganisms including 10 l, Aspergillua niger 5 l, Bacillus subtillis 5 l, and Saccharomyces cerevisiae 5 l are added to the mixture and mixed with 1 kg of glucose and 2 kg of molasses for 3 days at 25 ° C until pH 2 ~ 3. Culturing the above; Iv) Nitrosomonas 5 L of the liquid microorganisms of the culture composition is added to the extraction composition is secondary, and refrigerated to maintain 3 ~ 5 ℃ to mix; It characterized in that the natural sterilization disinfectant through.

In addition, it is characterized in that the natural disinfectant disinfectant prepared by the manufacturing method is diluted with 100 times the water and sprayed on the bottom of the barn.

In addition, it is characterized in that the natural disinfectant disinfectant prepared by the manufacturing method is diluted with 1000 times water and sprayed on the crop.

In addition, by diluting the natural disinfectant disinfectant 300 times with water prepared by the above production method is characterized in that for supplying drinking water to livestock.

Here, the finished natural disinfectant disinfectant decomposes manure by beneficial microorganisms when sprayed on the bottom of the barn, reducing odor, inhibiting bacterial invasion, and promoting digestion by disintegrating into easy-to-digest form even if eaten, thus raising feed efficiency. The period can be shortened.

On the other hand, when producing a natural disinfectant disinfectant, the composition ratio of each raw material can of course be added or subtracted within the normal range.

The present invention will be described in detail with reference to Examples and Experimental Examples, but these do not limit the scope of the present invention.

Example 1 Preparation of Natural Bactericidal Disinfectant

① Add 100 kg of mugwort, 80% of garlic, 5% of garlic, 5% of red pepper, and 10% of charcoal in a weight ratio, and add 10 kg of the material, and heat it to 100 ° C or higher to obtain 70 L of a composition.

② Filter the extract into a fine strainer and put the extracted composition into a microorganism incubator Lactobacillus sp. Four kinds of liquid microorganisms, including 10 l, Aspergillua niger 5 l, Bacillus subtillis 5 l, and Saccharomyces cerevisiae 5 l, are firstly mixed and mixed with 1 kg of glucose and 2 kg of molasses, and at least 3 days until pH2 to 3 at 25 ° C. Incubated.

③ 2 microliters of Nitrosomonas 5 L was added to the extraction composition in which the culture was completed and mixed. The natural sterilization disinfectant was completed by refrigeration to maintain a final temperature of 3 ~ 5 ℃.

All of the microorganisms were incubated with a concentrated fermenter, and the composition of the medium was referred to Bergey's manual of systematic Bacteriology Vol. I, II, III, IV. For specific microorganisms, refer to the contents of each research paper. And incubated.

Example 2 Spraying a Natural Disinfectant Disinfectant on Donsa's Floor

The natural bactericidal disinfectant completed in Example 1 was diluted with 100 times water and sprayed to the bottom of the house.

Example 3 A natural bactericidal disinfectant was diluted with water to feed livestock to drinking water

The natural bactericidal disinfectant completed in Example 1 was diluted with 300 times water and fed to drinking water for chicken or pig.

The natural bactericidal disinfectant of Example 2 prepared by such a manufacturing process was applied to the bottom of pigs to carry out the bacterium and antimicrobial effect of staphylococcus and salmonella, harmful emissions and specification tests, and Example 3 Intestinal antimicrobial effect test when fed natural sterilizing disinfectant to chicken or pig is as follows.

Experimental Example 1 Test of the Number and Staphylococcal Effect of Staphylococcus and Salmonella

1) Experimental group: Donsa of Example 2

2) Control Group: Conventional Sawdust Donsa

3) Test Items and Test Samples

Sample Number Sample Name Test Items Target strain 63-0803-16  Natural Disinfectant Disinfection Staphylococcus, Salmonella

4) Test period: 2008.03.10 ~ 2008.03.19

5) Test organization: Science and Technology Analysis Center

TABLE 1

Treatment group time Staphylococcus Salmonella Strain (CFU / mL) Antibacterial Effect (%) Strain (CFU / mL) Antibacterial Effect (%) Control Early 1.4 × 10 ⁵ - 5.3 × 10 ⁵ - 10 minutes 1.0 × 10 ⁵ - 4.0 × 10 ⁵ - 30 minutes 1.0 × 10 ⁵ - 4.0 × 10 ⁵ - 24 hours 7.8 × 10 ⁴ - 8.1 × 10 ⁵ - Experimental group Early 1.4 × 10 ⁵ - 5.3 × 10 ⁵ - 10 minutes 1.4 × 10 ⁴ 86.0 8.5 × 10 ⁴ 78.8 30 minutes 3.4 × 10 ³ 96.6 5.4 × 10 ³ 98.6 24 hours 7.3 × 10 ³ 90.6 6.3 × 10 ³ 99.2

However, the antimicrobial effect (%) of the test piece was calculated and calculated as follows.

Antimicrobial effect (%) = {(control bacteria-test bacteria) ㆇ control bacteria} ㅧ 100

As shown in Table 1, as a result of the test and the antimicrobial effect of Staphylococcus aureus and Salmonella in pigs, the experimental group to which the disinfectant was applied significantly reduced the number of Staphylococcus aureus and Salmonella bacteria causing disease infection and death compared to the control group. It was found to be excellent.

Experimental Example 2 Hazardous Exhaust Gas Test

1) Experimental group: Donsa of Example 2

2) Control Group: Conventional Sawdust Donsa

3) Measurer: Dr. Gil-Sil Eun, Ph.D., Veterinary Director

4) Measurement Date: 2006.12.07. 10:10

5) Measurer: Hokhom-myeon, Icheon-si, Gyeonggi-do

            Fourth money room at the entrance of Songam Farm

6) Swine condition: Sealed without ventilation due to winter heating

TABLE 2

Kinds Reference value Experimental group Control 2 cm 1m 2 cm 1m CO2 Less than 2500 750 ppm More than 5000ppm NH3 20 or less  15.7 ppm   14.8 ppm 156 ppm 141ppm H2S below 10 1.0-0.7ppm 1.6-0.9ppm 1.5-1.0ppm 2.1-1.8ppm

       ※ This measuring device can measure up to 5000ppm.

As shown in Table 2, as a result of the measurement test of the emissions of pigs, the experimental group to which the disinfectant was applied showed significantly lower carbon dioxide, ammonia, hydrogen sulfide, etc. in the pigs that cause the odor than the control group.

Experimental Example 3 Manure Discharge Test

1) Test subject and test number: 100 piglets total

2) Experimental group: Donsa of Example 2

3) Control Group: Conventional Sawdust Donsa

4) Swine status: Ventilate 1 ~ 3 days before mouth

TABLE 3

Kinds Harvest House area (pyeong) Breeding days Emission (Kg) Experimental group 50 heads 150 120 1,100 Control 50 heads 150 120 4,500

As shown in Table 3, ventilate for 1 to 3 days before the pig mouth, and then the piglets are extracted. The amount of excretion increases as pigs become adult, but when the natural fungicide of Example 1 is sprayed on the manure place little by little, the manure is completely decomposed, and as a result of the excretion test, the experimental group applied the natural disinfectant significantly reduced the amount of manure compared to the control group. heard.

Experimental Example 4 Specification Test

1) Test subject and test number: 113 pigs

2) Experimental group: Donsa of Example 2

3) Control group: automatic sludge pig yarn

TABLE 4

Kinds AB grade AB occurrence rate Training rate A grade Weight Shipping head Breeding days Experimental group 59 heads 86.4% 76.6% 41 6.770 59 165 days Control 62 heads 78.0% 76.7% 27 6.920 62  178 days

As shown in Table 4, as a result of the pig's specification test, the experimental group applied the natural disinfectant disinfectant to the barn floor was 13 days less than the control group and there was a significant difference even in the state of 3 fewer.

Experimental Example 5 Antibacterial Effect Test of Intestinal Bacteria by Natural Bactericidal Disinfectant

After hatching, 5 days old broiler chickens were stocked and divided into experimental and control groups, each with 3,000 animals and reared for 4 weeks. And the experimental group was fed the drinking water of Example 3 diluted natural water disinfectant of Example 1 with water, and the control group was fed general drinking water.

As a result, the experimental group fed natural bactericidal disinfectant suppressed the growth of Escherichia coli, Staphylococcus aureus and Salmonella causing the disease infection and mortality compared to the control group, resulting in reduced disease, high growth rate and low feed rate. Found to be excellent.

The natural bactericidal disinfectant of the present invention prepared in Example 1 was applied to the barn floor as in Example 2, and applied to broiler drinking water as in Example 3 to obtain the following effects.

1) Flesh-marking at the bottom of the barn No harmful gas is generated in the barn, but ammonia gas is suppressed by the genus Nitrosomonas, a beneficial microorganism.

2) Alcohol content produced by fermentation of the bottom of the barn can suppress bacterial invasion, and even if it is eaten, it is decomposed into an easy form for digestion, which improves digestion rate and shortens the breeding period by increasing feed efficiency, thus contributing to the farmer's income. Can be.

3) Even if the discharged manure decomposed by microorganisms is immediately sprayed on paddy fields or fields, it is possible to realize eco-friendly organic farming without harm to crops.

4) In addition, it can replace pesticides when spraying on crops by diluting with water, which can contribute to income increase of farmers and livestock farmers.

5) Salmonella, an intestinal bacterium, is inhibited from proliferation, resulting in low disease incidence and high growth rate.

When the natural disinfectant disinfectant of the present invention is diluted with water and sprayed on the barn, it can reduce staphylococcus and Salmonella causing various diseases and mortality, and harmful carbon dioxide generated during rapid extinction of livestock excretion and decomposition of excreta. Improve the environment in the farming area by minimizing the occurrence of ammonia, ammonia and hydrogen sulfide, so that the hygiene and decomposed manure can be composted immediately, and can replace pesticides when sprayed on crops, and reduce mortality by suppressing intestinal pathogens of livestock. By increasing the rate of increase and shortening the shipping period, it can contribute to the income increase of farmers and livestock farmers.

Claims (5)

Ⅰ) To 100 liter of purified water, add 70 kg of mugwort, 4-10% of garlic, 4-10% of red pepper, 7-10% of charcoal, and add 10 kg of mixed material in weight ratio Obtaining; Ii) Filtering the extract with a fine filter net, and then put the extracted composition into a microorganism incubator Lactobacillus sp. Four kinds of liquid microorganisms including 10 l, Aspergillua niger 5 l, Bacillus subtillis 5 l, and Saccharomyces cerevisiae 5 l are added to the mixture and mixed with 1 kg of glucose and 2 kg of molasses for 3 days at 25 ° C until pH 2 ~ 3. Culturing the above; Iv) Nitrosomonas 5 L of the liquid microorganisms of the culture composition is added to the extraction composition is secondary, and refrigerated to maintain 3 ~ 5 ℃ to mix; Method for producing a natural disinfectant disinfectant comprising Natural disinfectant disinfectant prepared by the method of claim 1  Natural sterilizing disinfectant for livestock used in claim 2 to dilute the finished natural disinfecting disinfectant with water 100 times and spray it to the bottom of the house. The natural disinfectant disinfectant for crops, which is diluted with 1000 times of water and sprayed on the crops, according to claim 2 The natural disinfectant disinfectant for livestock according to claim 2, wherein the natural disinfectant disinfectant is diluted with 300 times water and supplied to the animal in a negative manner.