CN1951449B - Medicament for increasing peripheral blood leukocyte and use thereof - Google Patents
Medicament for increasing peripheral blood leukocyte and use thereof Download PDFInfo
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- CN1951449B CN1951449B CN 200610016117 CN200610016117A CN1951449B CN 1951449 B CN1951449 B CN 1951449B CN 200610016117 CN200610016117 CN 200610016117 CN 200610016117 A CN200610016117 A CN 200610016117A CN 1951449 B CN1951449 B CN 1951449B
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- peripheral blood
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Abstract
The invention discloses a medicament for increasing patients' white cells and its use, wherein the medicament is prepared from matrimony vine extract and mushroom extract by the weight ratio of 1-4:4:1. The medicament has the function of protecting immunological organs and alleviating injury of chemotherapy medicament side effect to human body.
Description
Technical field
The present invention relates to a kind of pharmaceutical composition, particularly relate to a kind of medicine and purposes of increasing peripheral blood leukocyte.
Background technology
Polysaccharide is the polymer that is formed by connecting by monosaccharide.Since the seventies in 20th century, scientists finds that polysaccharide and saccharide complex participate in and mediated the adjusting of the adjusting, particularly immunologic function of the various biosiss of cell.Studies show that polysaccharide especially herbal polysaccharide has pharmacological actions such as enhancing human body immunity function and antitumor, and does not almost have toxicity.Therefore herbal polysaccharide more and more causes domestic and international pharmacologist, biologist and chemists' interest, becomes current research focus.Former studies is to carry out on single polysaccharide mostly, and several polysaccharide are used in combination, and does not still have reported in literature.In addition, polysaccharide composition is given birth to influence to the peripheral white blood cell volume production does not have reported in literature yet.
In various degree cell function damage and immune organ damage can take place in tumour patient in chemotherapy, radiotherapy process, see that especially the weight of main immune organ such as peripheral blood WBC quantity reduction and spleen, thymus etc. reduces.
Therefore, seek a kind of energy increasing peripheral blood leukocyte, the medicine of human body immunity improving power and protection immune organ is a problem demanding prompt solution.
Summary of the invention
The medicine that the purpose of this invention is to provide a kind of energy increasing peripheral blood leukocyte.
Second purpose of the present invention provides a kind of purposes of medicine of energy increasing peripheral blood leukocyte.
Technical scheme of the present invention is summarized as follows:
A kind of medicine that can increasing peripheral blood leukocyte is that wolfberry fruit extract and the Lentinus Edodes extract of 1-4: 1-4 formed by weight ratio.
Preferably: by weight ratio is that wolfberry fruit extract and the Lentinus Edodes extract of 2-3: 2-3 formed.
Preferably: by weight ratio is that 3: 2 wolfberry fruit extract and Lentinus Edodes extract formed.
A kind of purposes of medicine of energy increasing peripheral blood leukocyte is characterized in that the application at the medicine of preparation increasing peripheral blood leukocyte.
Advantage of the present invention is: the medicine of a kind of energy increasing peripheral blood leukocyte of the present invention has immunoregulation effect; in effect aspect rising peripheral blood leucocyte and the protection immune organ significantly and be better than single polysaccharide, can alleviate the injury of chemotherapeutics side effect to human body.
Description of drawings
Fig. 1 is Fructus Lycii of the present invention and Lentinus Edodes extraction process flow chart.
The specific embodiment
The present invention is further illustrated below in conjunction with specific embodiment:
Embodiment 1
A kind of medicine that can increasing peripheral blood leukocyte is to get the wolfberry fruit extract of 3 weight portions and the Lentinus Edodes extract of 2 weight portions is mixed and made into.Fructus Lycii is preferably selected lycium barbarum for use.
Wolfberry fruit extract and Lentinus Edodes extract are preparation technology shown in Figure 1 and make.
Embodiment 2
A kind of medicine that can increasing peripheral blood leukocyte is to get the wolfberry fruit extract of 1 weight portion and the Lentinus Edodes extract of 4 weight portions is mixed and made into.
Embodiment 3
A kind of medicine that can increasing peripheral blood leukocyte is to get the wolfberry fruit extract of 4 weight portions and the Lentinus Edodes extract of 1 weight portion is mixed and made into.
Embodiment 4
A kind of medicine that can increasing peripheral blood leukocyte is to get the wolfberry fruit extract of 2 weight portions and the Lentinus Edodes extract of 3 weight portions is mixed and made into.
Embodiment 5
A kind of medicine that can increasing peripheral blood leukocyte is to get the wolfberry fruit extract of 1 weight portion and the Lentinus Edodes extract of 1 weight portion is mixed and made into.
Embodiment 6
The medicine of observing a kind of energy increasing peripheral blood leukocyte of the present invention is to effect of immunologic function
1 material and instrument
1.1 wolfberry fruit extract in the medicine of a kind of energy of medicine increasing peripheral blood leukocyte and Lentinus Edodes extract are by Inst. of Hygienics and Environmental Medical Science, Academy of Military Medici's separation, purification gained (preparation technology's flow process is seen Fig. 1), and detect through authoritative institution, be brown fine powder, polyoses content is greater than 50% in the medicine; Positive control drug is produced by Tianjin pharmaceutcal corporation, Ltd, and the vegetable active polysaccharide is defended No. the 0223rd, food key word (2001).Two medicines are all used distilled water diluting.
1.2 animal Kunming mouse male and female half and half, in 6～8 weeks of age week, body weight 18～22g is provided by Military Medical Science Institute's Experimental Animal Center.
1.3 reagent and instrument Cyclophosphamide for injection (Cy), Hualian Pharmaceutical Co., Ltd., Shanghai's product, the accurate word H31021703 of traditional Chinese medicines, lot number 050109, leukocyte diluent, platelet diluent, 0.9% normal saline; The XSZ-D2 inverted microscope, blood counting chamber.
2 methods and result
2.1 experimental technique
Get 100 of mices, body weight 18～22g, male and female half and half evenly are divided into 10 groups at random.Wherein 8 groups is experimental group: (dosage is 50mgKg to make CY immunocompromised model
-1D
-1), continuous 7 days lumbar injection 0.3ml gavage large and small dosage lycium barbarum polysaccharide LBP (300～400) mgKg respectively
-1D
-1Large and small dosage lentinan LNT (300～400) mgKg
-1D
-1Medicine (embodiment 1) (200～300) mgKg of large and small dosage a kind of energy increasing peripheral blood leukocyte of the present invention
-1D
-1Large and small dosage positive control (200～300) mgKg
-1D
-1Administration every day 1 time, each 0.5ml, continuous 10 days.Model group: gavage equal-volume distilled water, injection equal-volume cyclophosphamide.Normal group: gavage equal-volume distilled water, injection equal-volume normal saline.
Each treated animal was handled in the 11st day: extract eyeball and get blood, survey peripheral blood WBC quantity; Broken end is won thymus, spleen, inhales on the rearmounted balance of branch that anhydrates with filter paper and weighs, and calculates organ index;
(X ± s) the t check is adopted in expression to experimental data with mean ± standard deviation.
2.2 the result descends to the murine interleukin number that is caused by cyclophosphamide and the influence of immune organ damage
2.2.1 the medicine of a kind of energy increasing peripheral blood leukocyte of the present invention is to the effect of peripheral blood leucocyte
Each treated animal peripheral blood leucocyte sum sees Table 1.By table 1 as seen: model group WBC quantity is starkly lower than normal group (P＜0.01).Illustrate that CY can cause that mice peripheral blood WBC reduces with chemotherapeutics CY modeling success, this is one of common side effect of chemotherapeutics; The medicine group of a kind of energy increasing peripheral blood leukocyte of the present invention and model group relatively have significant difference, illustrate that the medicine of a kind of energy increasing peripheral blood leukocyte of the present invention has the effect of tangible rising peripheral blood WBC; The single polysaccharide group of large and small dosage and model group compare, there was no significant difference, and it is not obvious to rise white effect; Positive controls and model group rating unit are variant; Therefore, of the present invention a kind of can increasing peripheral blood leukocyte medicine in effect aspect the rising mice peripheral blood WBC significantly and be better than single polysaccharide.
The medicine of table 1. a kind of energy increasing peripheral blood leukocyte of the present invention is to the effect of peripheral blood leucocyte
*P＜0.05，
**P＜0.01
2.2.2 the medicine of a kind of energy increasing peripheral blood leukocyte of the present invention is to the effect of spleen and thymus
By table 2 as seen, the CY lumbar injection after 7 days thymus and index and spleen index obviously reduce, model group and normal group have significant difference (P＜0.01), illustrate that its immune organ weight obviously reduces; The medicine group of a kind of energy increasing peripheral blood leukocyte of the present invention and model group are relatively, significant difference (P＜0.05) is arranged, illustrate that the medicine of a kind of energy increasing peripheral blood leukocyte of the present invention has protective effect to the immune organ damage that CY causes; The monosaccharide group of large and small dosage and model group compare, there was no significant difference; Positive controls and model group are more effective than major part; Therefore, a kind of medicine that can increasing peripheral blood leukocyte of the present invention is improving aspect the important immune organ of mice effect significantly and be better than single polysaccharide.
The medicine of table 2. a kind of energy increasing peripheral blood leukocyte of the present invention is to the effect of spleen and thymus
*P＜0.05，
**P＜0.01
3. discuss
Lycium barbarum polysaccharide and lentinan have immune widely pharmacological action.In the past studies have shown that both all have tangible immunostimulant and immune organ protective effect, this research is carried out scientific composition to two kinds of polysaccharide on the basis of single polysaccharide, proved that polysaccharide possesses the synergic effect of decrement after composite.
In various degree cell function damage and immune organ damage can take place in tumour patient in chemotherapy, radiotherapy process, see that especially the weight of main immune organ such as peripheral blood WBC quantity reduction and spleen, thymus etc. reduces.This research is model with the kunming mice that CY handles, and carries out comparative study on single polysaccharide, two factor various dose of compound polyose level, and does positive control.The result shows that this preparation effect aspect rising peripheral blood WBC quantity, protection immune organ damaged is remarkable, for the preparation of studying chemotherapeutics side effect aspect lays the first stone.
Embodiment 7
Employing is carried out immune function experiment with embodiment 6 identical methods to embodiment 2, embodiment 3, embodiment 4, embodiment 5.The result shows and the matched group comparison all has significant difference in rising peripheral blood leucocyte and protection aspect the immune organ, points out these several prescriptions to be better than matched group aspect immunomodulating.
The medicine of table 3. a kind of energy increasing peripheral blood leukocyte of the present invention is to the effect of peripheral blood leucocyte
*P＜0.05，
**P＜0.01
The medicine of table 4. a kind of energy increasing peripheral blood leukocyte of the present invention is to the effect of spleen and thymus
*P＜0.05，
**P＜0.01
Embodiment 8
Wolfberry fruit extract: 162g (54%)
Lentinus Edodes (Lentinus Edodes) extract: 108g (36%)
Maltodextrin: 30g (10%)
300g
Make 1000 capsules
Eating method and amount: oral on an empty stomach; Adult every day 2 times, each 3-4 grain
The child reduces by half and takes
Every day, dosage 1.8-2.4 restrained
Claims (1)
1. the medicine of an energy increasing peripheral blood leukocyte is in the application of the medicine of preparation increasing peripheral blood leukocyte, it is characterized in that described medicine that can increasing peripheral blood leukocyte is that wolfberry fruit extract and the Lentinus Edodes extract of 1-4: 1-4 formed by weight ratio, polyoses content is greater than 50% in the described medicine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610016117 CN1951449B (en) | 2006-10-09 | 2006-10-09 | Medicament for increasing peripheral blood leukocyte and use thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610016117 CN1951449B (en) | 2006-10-09 | 2006-10-09 | Medicament for increasing peripheral blood leukocyte and use thereof |
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| Publication Number | Publication Date |
|---|---|
| CN1951449A CN1951449A (en) | 2007-04-25 |
| CN1951449B true CN1951449B (en) | 2011-02-09 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 200610016117 Expired - Fee Related CN1951449B (en) | 2006-10-09 | 2006-10-09 | Medicament for increasing peripheral blood leukocyte and use thereof |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104872656B (en) * | 2015-03-06 | 2019-01-15 | 江中（天津）健康科技发展有限公司 | A kind of composition with health care function and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1203044A (en) * | 1997-08-20 | 1998-12-30 | 陈炳炎 | Formula of Chinese medicine beverage |
| CN1704085A (en) * | 2004-06-04 | 2005-12-07 | 成都地奥制药集团有限公司 | Pharmaceutical composition with immunoregulation function and method for preparing the same |
| CN1970005A (en) * | 2006-10-09 | 2007-05-30 | 中国人民解放军军事医学科学院卫生学环境医学研究所 | Medicament for improving anoxia tolerance and use thereof |
-
2006
- 2006-10-09 CN CN 200610016117 patent/CN1951449B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1203044A (en) * | 1997-08-20 | 1998-12-30 | 陈炳炎 | Formula of Chinese medicine beverage |
| CN1704085A (en) * | 2004-06-04 | 2005-12-07 | 成都地奥制药集团有限公司 | Pharmaceutical composition with immunoregulation function and method for preparing the same |
| CN1970005A (en) * | 2006-10-09 | 2007-05-30 | 中国人民解放军军事医学科学院卫生学环境医学研究所 | Medicament for improving anoxia tolerance and use thereof |
Non-Patent Citations (3)
| Title |
|---|
| 倪峰.免疫活性的中药多糖.福建中医学院学报11 1.2001,11(1),57-58. |
| 倪峰.免疫活性的中药多糖.福建中医学院学报11 1.2001,11(1),57-58. * |
| 董玉新,赵晓华.香菇枸杞复合饮料的研制.浙江食用菌.1997,32-33. * |
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| CN1951449A (en) | 2007-04-25 |
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