CN1951187A - Preparation for preventing and treating citrus canker - Google Patents
Preparation for preventing and treating citrus canker Download PDFInfo
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- CN1951187A CN1951187A CN 200510109169 CN200510109169A CN1951187A CN 1951187 A CN1951187 A CN 1951187A CN 200510109169 CN200510109169 CN 200510109169 CN 200510109169 A CN200510109169 A CN 200510109169A CN 1951187 A CN1951187 A CN 1951187A
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- xanthomonas campestris
- exocellular polysaccharide
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Abstract
The invention relates to an agent used to prevent ulcer of citrus tree. Wherein, it comprises wide rape external polysaccharide (xanthan gum), yellow granulated sugar, ammonia sulfate, and blue copperas to be mixed and added water into paste, while the mixture and water are rationed at 1:4; in microwave oven, heating the paste to be boiled for 5mins, to crosslink the external polysaccharide with copper ion to form yellow paste copper agent; before using, adding water into said agent to dilute it to 250 times to form liquid agent; when using, putting liquid agent into sprayer, to spray the branch and seed of citrus tree; and spraying at each 10 days; spraying three times at all. The invention has simple process and low cost.
Description
Technical field
The present invention relates to the control of orange tree disease, relate in particular to the control of the citrus ulcer that causes by the pathogenic mutation of carpetweed Xanthomonas campestris citrus.
Background technology
Citrus ulcer is a kind of quarantine disease that the Citrus fruit tree is had catastrophic collapse.In recent years, because the carelessness that the blindness of nursery stock allocation and transportation are introduced a fine variety and produced, the citrus ulcer in citrus producing region, various places has the rising spreading trend, should draw attention on producing, in order to avoid nuisanceless commercialization production of various places citrus and formation for export are threatened.According to statistics, the annual direct economic loss that causes because of citrus ulcer is seriously restricting the development of China's citrus industry up to more than 500,000,000 yuan.The pathogen of citrus ulcer is the pathogenic mutation of carpetweed Xanthomonas campestris citrus, and this bacterium can be infected nearly all citrus variety, and main harm citrus leaves, branch and fruit produce the ulcer scab.Faint yellow oil stain shape fleck occurs at blade back when the blade of being injured begins, develop into subcircular, filbert main brown scab then, blade disease portion positive and negative projection has the yellow green haloing on every side more, and sinking and breaks in later scab center, is the ulcer shape; The branch scab does not show halo, is dark brown glaze limit, and the fruit scab is similar to blade; Endanger and cause fallen leaves, deadwood and shedding when serious; Nursery stock and treelet are injured and cause setting body death when serious.How wet condition can promote this disease to break out to high temperature, and the preference temperature that pathogen grows is 20 ℃～30 ℃; Germ intrudes into new strain tissue and needs the wet condition of water, and general moisture film keeps more than 20 minutes; Germ generally can be invaded from wound, pore and hole skin etc.Usually, citrus nursery stock and earsh should diseases than easy infection, orange class is easily susceptible, nitrogenous fertilizer too much or the morbidity of the orchard of extensive management type can be more serious, the harm of leaf miner also is the main cause that causes citrus ulcer seriously to break out and infect.At present, main prophylactico-therapeutic measures comprises: set up seedling quarantine system, to the seedling disinfection, guarantee to use nontoxic seedling, rational deployment orchard, pay attention to cultivation management and the rational application of fertilizer and medication in good time.Main medicament comprises bordeaux mixture, network ammonia copper, copper rosinate, copper oxychloride, copper acetate, chlorine breast copper, mancozeb, agricultural streptomycin etc., and general control efficiency only is 60%～80%, and, mantoquita water-soluble too high, easily by the rainwater leaching loss, cause drug effect short, environmental pollution is bigger.The long-term agricultural streptomycin that uses not only easily makes phytopathogen produce resistance, also may comprise between human pathogen and expanding because the horizontal transfer of gene causes pesticide resistance multiple pathogen.
In view of this, the control of citrus ulcer is needed badly a kind ofly can be improved control efficiency effectively, can reduce crop and environment are on every side produced harmful effect and be difficult for medicament that phytopathogen is developed immunity to drugs again.
Summary of the invention
The present invention is for solving the problem that exists in the above-mentioned citrus ulcer Prevention Technique, and process discovers that the exocellular polysaccharide of xanthomonas campestris can evoke plant defense to a certain extent, thereby can obviously improve the degeneration-resistant and immunocompetence of plant self.Therefore, the objective of the invention is this discovery is applied to the control of citrus ulcer, exocellular polysaccharide preparation that a kind of xanthomonas campestris is provided and preparation method thereof reaches using method.
According to a goal of the invention of the present invention, a kind of exocellular polysaccharide preparation of xanthomonas campestris is provided, the said preparation prescription is: the mixture that exocellular polysaccharide (xanthans), brown sugar, ammonium sulfate, the copper sulphate geometric ratio of xanthomonas campestris is mixed, promptly, respectively get the equal portions mixing by weight of material.The ratio of this mixture and water is to add water at 1: 4.
The exocellular polysaccharide of xanthomonas campestris can be the commodity xanthans in the said preparation prescription, also can be the thick xanthans of xanthomonas campestris fermented extracted.
According to another object of the present invention, a kind of preparation method of exocellular polysaccharide preparation of xanthomonas campestris is provided, the steps include: the exocellular polysaccharide (xanthans) of xanthomonas campestris and brown sugar, ammonium sulfate, the copper sulphate geometric ratio formation mixture that is mixed; Add water in this mixture, the ratio of this mixture and water is 1: 4; This mixture is modulated into the lotion of scattered paste shape with water; To this lotion heating, make this lotion with low fire to little boiling back 5 minutes, so that the exocellular polysaccharide of this xanthomonas campestris and copper ion are cross-linked to form yellow glue copper system; To this yellow glue copper preparation thin up to 250 times, be convenient to spraying and use.
According to another purpose of the present invention, a kind of using method of exocellular polysaccharide preparation of xanthomonas campestris is provided, the steps include: the exocellular polysaccharide preparation of xanthomonas campestris is placed a sprayer; Young sprout and young fruit period fruit tree to orange tree are sprayed first at citrus ulcer premorbid or their early stage; And the spraying once more in 10 days of every interval needs spraying 3 times altogether.
According to another goal of the invention of the present invention, the preparation that will have the exocellular polysaccharide composition of this xanthomonas campestris is applied to the control of citrus ulcer.
The preparation that the present invention will have an exocellular polysaccharide composition of xanthomonas campestris is applied to the control of citrus ulcer, because the exocellular polysaccharide composition of this xanthomonas campestris can evoke plant defense, obviously improves the degeneration-resistant and immunocompetence of plant self; Secondly, the bactericidal action of copper ion; Have again,, in solution, exist, spray and behind plant surface, form the micropore diaphragm, have water conservation, prevent the effect that germ is infected with space network through hot crosslinked mixture.Therefore, under said preparation shows remarkable role in synergy, can improve the control efficiency (reaching more than 80%) of citrus ulcer effectively; And the use of the exocellular polysaccharide preparation of this xanthomonas campestris can not produce any harmful effect to crop and environment on every side, and is difficult for making phytopathogen to develop immunity to drugs; And its manufacturing is simple, easy to use, low cost and other advantages more can impel the Prevention Technique of citrus ulcer to obtain significant progress.
Embodiment
The exocellular polysaccharide pharmaceutical formulation of xanthomonas campestris: exocellular polysaccharide of xanthomonas campestris (xanthans) and the mixture that brown sugar, ammonium sulfate, copper sulphate geometric ratio are mixed, promptly by weight of material, respectively get 50 grams, mixing.The ratio of this mixture and water is 1: 4, adds 800 gram water (0.8 liter).
The preparation method of the exocellular polysaccharide preparation of xanthomonas campestris: exocellular polysaccharide of xanthomonas campestris (xanthans) and the mixture that brown sugar, ammonium sulfate, copper sulphate geometric ratio are mixed, promptly by weight of material, respectively get 50 grams, mixing.The ratio of this mixture and water is 1: 4, adds 800 gram water (0.8 liter).With the lotion of water with this mixture modulation scattered paste shape; In micro-wave oven, make this lotion to little boiling back 5 minutes with low fire heating, so that the exocellular polysaccharide of this xanthomonas campestris and copper ion are cross-linked to form the yellow glue copper preparation, the preparatory processing formulation is a lotion; To this yellow glue copper preparation thin up to 250 times, so that spraying is used.
The using method of the exocellular polysaccharide preparation of xanthomonas campestris: the steps include: the exocellular polysaccharide preparation of xanthomonas campestris is placed a sprayer; Young sprout and preceding or their early stage (inoculation back) spraying first of young fruit period fruit tree to orange tree in citrus ulcer morbidity (inoculation); And the spraying once more in 10 days of every interval needs spraying 3 times altogether.
According to prescription, preparation method and the using method of the exocellular polysaccharide preparation of above-mentioned xanthomonas campestris, the control efficiency of obtained citrus ulcer sees also subordinate list.The effect of inventive embodiments obviously is better than agricultural streptomycin, copper sulphate, is better than mancozeb, bordeaux mixture slightly.
Subordinate list yellow glue copper preparation is to Liu Sha orange spring tip leaf ulcer prevention spraying experimental result (30 days rear blade scab numbers)
A
| 10 leaf disease spot numbers (individual/10 leaf) B | ||
| Dispenser before the inoculation C | Inoculation back dispenser D | |
| Contrast (clear water) | 97 | 176/10 leaves D |
| The embodiment of the invention | 5 | 21 |
| Copper sulphate (500 times) | 28 | 56 |
| Bordeaux mixture (500 times) | 7 | 23 |
| Mancozeb (80%, 1000 times of liquid) | 12 | 29 |
| Agricultural streptomycin (2000 times) | 39 | 72 |
Annotate:
A. every blade artificial infection carpetweed Xanthomonas campestris citrus 10 points of mutation pathogen that cause a disease.
B.10 sheet leaf disease spot number.
C. inoculate the dispenser first time in preceding 24 hours, the morbidity of this inoculation expression citrus ulcer.
D. inoculate the dispenser first time in back 24 hours, the morbidity of this inoculation expression citrus ulcer.
The preparation of the exocellular polysaccharide preparation of xanthomonas campestris provided by the invention has makes advantages such as simple, easy to use, that cost is low and have no side effect, can effectively prevent and treat the generation of citrus bacterial canker disease, be suitable in China's oranges and tangerines production, the control of citrus bacterial canker disease.
Claims (9)
1, a kind of exocellular polysaccharide preparation of xanthomonas campestris, it is characterized in that, the said preparation prescription is: the mixture that the exocellular polysaccharide of xanthomonas campestris (xanthans), brown sugar, ammonium sulfate, copper sulphate geometric ratio are mixed, add water, and the ratio of this mixture and water is 1: 4.
2, a kind of preparation method of exocellular polysaccharide preparation of xanthomonas campestris is characterized in that, this preparation method's step is:
(1) exocellular polysaccharide (xanthans) with xanthomonas campestris is mixed with brown sugar, ammonium sulfate, copper sulphate geometric ratio;
(2) add water in this mixture, the ratio of this mixture and water is 1: 4;
(3) this mixture is modulated into the lotion of scattered paste shape with water;
(4) with low fire this lotion is heated, make this lotion to little boiling back 5 minutes, so that the exocellular polysaccharide of this xanthomonas campestris and copper ion are cross-linked to form the yellow glue copper preparation.
According to the described preparation method of claim 2, it is characterized in that 3, this preparation method also comprises step: to this yellow glue copper preparation thin up to 250 times.
4, a kind of using method of exocellular polysaccharide preparation of xanthomonas campestris is characterized in that, the step of this using method is:
(1) the exocellular polysaccharide preparation to xanthomonas campestris places a sprayer;
(2) phase of using is carried out the 1st spraying first to orange tree object Yu Yi; Thereafter, the spraying once more in 10 days of every interval needs spraying 3 times altogether.
According to the described using method of claim 4, it is characterized in that 5, wherein, described orange tree object is the young sprout of orange tree.
6, according to the described using method of claim 4, it is characterized in that, wherein, the young fruit period fruit tree that described orange tree object is a Citrus.
According to the described using method of claim 4, it is characterized in that 7, wherein, the described phase of using first is citrus ulcer premorbid period.
According to the described using method of claim 4, it is characterized in that 8, wherein, the described phase of using first is the citrus ulcer their early stage.
9, the preparation that will have an exocellular polysaccharide composition of this xanthomonas campestris is applied to the control of citrus ulcer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005101091691A CN100407920C (en) | 2005-10-20 | 2005-10-20 | Preparation for preventing and treating citrus canker |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005101091691A CN100407920C (en) | 2005-10-20 | 2005-10-20 | Preparation for preventing and treating citrus canker |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1951187A true CN1951187A (en) | 2007-04-25 |
| CN100407920C CN100407920C (en) | 2008-08-06 |
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ID=38057907
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005101091691A Expired - Fee Related CN100407920C (en) | 2005-10-20 | 2005-10-20 | Preparation for preventing and treating citrus canker |
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| Country | Link |
|---|---|
| CN (1) | CN100407920C (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101260399B (en) * | 2008-03-13 | 2011-08-10 | 湖南农业大学 | Orange canker resistant pthA-nls gene and its construction method and application |
| WO2011065831A3 (en) * | 2009-11-27 | 2011-09-01 | Ceradis B.V. | Improved formulations comprising heteropolysaccharides |
| CN105153140A (en) * | 2015-11-02 | 2015-12-16 | 许自协 | Compound for inhibiting growth of xanthomonas citri |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1027033C (en) * | 1991-12-10 | 1994-12-21 | 罗顺意 | New chemicals for prevention and cure of orange and tangerine ulcer and preparation thereof |
-
2005
- 2005-10-20 CN CNB2005101091691A patent/CN100407920C/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101260399B (en) * | 2008-03-13 | 2011-08-10 | 湖南农业大学 | Orange canker resistant pthA-nls gene and its construction method and application |
| WO2011065831A3 (en) * | 2009-11-27 | 2011-09-01 | Ceradis B.V. | Improved formulations comprising heteropolysaccharides |
| CN105153140A (en) * | 2015-11-02 | 2015-12-16 | 许自协 | Compound for inhibiting growth of xanthomonas citri |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100407920C (en) | 2008-08-06 |
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Granted publication date: 20080806 Termination date: 20101020 |