CN1923265B - Application of wheat germ oil in pharmaceutical preparation for preventing and treating osteoporosis - Google Patents
Application of wheat germ oil in pharmaceutical preparation for preventing and treating osteoporosis Download PDFInfo
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- CN1923265B CN1923265B CN200610110559A CN200610110559A CN1923265B CN 1923265 B CN1923265 B CN 1923265B CN 200610110559 A CN200610110559 A CN 200610110559A CN 200610110559 A CN200610110559 A CN 200610110559A CN 1923265 B CN1923265 B CN 1923265B
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Abstract
The invention discloses a wheat germ oil preparation for the prevention and treatment of osteoporosis and skin senility, wherein the preparation can be made into tablets, capsules, granules, electuaries, oral liquids, externally preparations, injections and any clinically applicable pharmaceutical preparations.
Description
Technical field the present invention relates to wheat germ oil is used for the pharmaceutical preparation of protect against osteoporosis and skin aging and health and beauty in preparation application.
Background technology health and longevity are the life sciences Eternal Theme.Along with the acceleration aging of world population, research on anti-senescence has become an applied science of rapid emergence.The position the earliest of human senility is osseous tissue and skin.Medical research proves: the aging of people's bone after 20 years old has just slowly begun, and after 50 years old, osteoporosis is accelerated greatly, and causes fracture easily.Osteoporosis makes one of old and feeble important symbol, and another is exactly a skin aging, and skin aging is the part of organism aging process, and wearing out of body shows the clearlyest on skin.Protect against osteoporosis and skin aging have become the focus of medical scientific, but few people connect the two.We are in the research process of defying age and osteoporosis, find that several plant oils has the osteoporotic effect of good preventing, the function of excellent prevention skin aging is also arranged simultaneously, and this several plant oils is Radix Oenotherae erythrosepalae oil, Fructus Perillae oil, wheat germ oil, walnut oil, safflower oil.
This project of summary of the invention seminar finds the effect that several plant oils wheat germ oil, Radix Oenotherae erythrosepalae oil, Fructus Perillae oil, walnut oil, safflower oil have protect against osteoporosis, simultaneously, the effect that has the prevention skin aging again, can be used for preparing the pharmaceutical preparation of protect against osteoporosis, also can be used for preparing the preparation that prevents skin aging.
Above-mentioned vegetable oil can be made into tablet, capsule, granule, electuary, oral liquid, external preparation, injection and any for clinical practice pharmaceutical preparation.
The specific embodiment:
1. experimental technique
1.1 laboratory animal and raising condition
Three 70 of monthly age regular grade Sprangue-Dawley (SD) female rats (Guangdong Medical College's Experimental Animal Center provides), body weight 225 ± 12g.The raising condition: raise indoor temperature and keep 24 ℃ ~ 28 ℃, humidity is 50% ~ 60%.Divide cage common nursing.Two in every cage, the water of freely ingesting, specially raise the chamber, and the special messenger is responsible for, and weighs once weekly.Animal feeding and experiment three totally months.
1.2 research medicine and compound method
(1) diethylstilbestrol: Guangdong Bidi Pharmaceutical Co., Ltd; Compound method: diethylstilbestrol 2mg, distilled water 1000ml, the dissolving of heating gets final product.
(2) four vegetable oil
Refinement of evening primrose oil, refining Fructus Perillae oil, refining wheat germ oil, refining walnut oil is all purchased the dense magnificent biological engineering company limited in Dalian, and the main constituent of these several oil sees Table 1:
Table 1: the content of the main constituent of several oil
1.3. experimental technique
Set up the senile osteoporosis of estrogen deficiency and the rat animal model of skin aging with the oophorectomize method, control rats adopts the sham-operation method, perform an operation equally when promptly testing, but spay not, as experiment contrast.Experiment grouping administration situation sees Table 1:
Table 1: grouping of medicine osteoporosis research experiment and administration situation
1.4. castration of animal and administration
The castration of animal: except that the sham operated rats rat, surplusly respectively organize rat and anaesthetize (0.12ml/kg) with 3% pentobarbital, under the strict sterilization condition, the operation of row ovariectomized rats was taken out stitches after one week.The sham operated rats rat is opened the abdominal cavity in same area (both sides abdominal part) and is not extractd ovary and sew up immediately.
Administration: sham operated rats and oophorectomize group per os every day are irritated and are fed normal saline 5ml*kg-1*d-1, and diethylstilbestrol group per os every day is irritated and fed diethylstilbestrol 10ug kg-1*d-1; Radix Oenotherae erythrosepalae line of oils, Fructus Perillae oil group, wheat germ line of oils, Semen Juglandis line of oils per os every day are irritated respectively and are fed Radix Oenotherae erythrosepalae oil, Fructus Perillae oil, wheat germ oil, walnut oil 5ml*kg-1*d-1.Successive administration three months.
1.5. draw materials
Behind the successive administration three months, under the anesthesia of 3% pentobarbital, rat is put to death in the right ventricle blood drawing, and separation of serum is made serum Ca and measured.Get the left side ulna, Ex-all muscle and soft tissue, 80 ℃ of baking 48h get key weight to constant weight, then in 6molL
-1HCl, digestion 16h after-filtration is got filtrate under 108 ℃ of temperature, measure calcium (Ca), phosphorus (P) content in the bone with ICP (inductance coupled plasma direct-reading spectrometer), and bone hydroxyproline (Hyp) content (the hydroxyproline test kit is that bio-engineering corporation's product is built up in Nanjing).Separate the right side tibia, open pulp cavity as sagittal, put into 10% formalin buffer and fix with sawing at near-end at a slow speed, dehydration then, plastic embedding is done in defat.Downcut undecalcified 4 μ m and 8 μ m section respectively with sclerous tissues's microtome, 4 μ m section Goldner ' s Trichrome dyeing mounting carries out bone meterological static parameter (static parameters) and measures.The 8 μ m section mounting that do not dye carries out bone meterological dynamic parameter (dynamic parameters) as Fluirescence observation and measures.
2. experimental result
The result of bone meterological static parameter as seen, the removal ovary rat is compared with control rats, the bone amount is obviously lost after 90 days, the bone trabecula number reduces (P＜0.01), the bone trabecula separating degree increases (P＜0.01), the osteoclast number increases (P＜0.01).Diethylstilbestrol makes bone amount of removal ovary rat and bone trabecula number obviously increase (P＜0.01), bone trabecula separating degree and osteoclast number obviously to reduce.Radix Oenotherae erythrosepalae oil, Fructus Perillae oil, wheat germ oil, walnut oil all can make the bone amount of removal ovary rat that increase (P＜0.05) is arranged, but with diethylstilbestrol group indifference.
The result of bone meterological dynamic parameter as seen, the ovariectomized group rat bone forms index and obviously increases: the mineralising deposition increases (P＜0.05), bone formation rate BFR/BV increases (P＜0.05).Diethylstilbestrol can suppress the bone formation rate (BFR/BV, P＜0.05) of removal ovary rat.Radix Oenotherae erythrosepalae oil, Fructus Perillae oil, wheat germ oil, walnut oil all form index to the removal ovary rat bone does not have obvious influence.
More than studies show that, Radix Oenotherae erythrosepalae oil, Fructus Perillae oil, wheat germ oil, walnut oil has tangible preventive and therapeutic effect to the experimental animal models osteoporosis.
Claims (1)
1. wheat germ oil is used for preventing and treating the application of the pharmaceutical preparation of the osteoporosis that oophorectomize causes in preparation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200610110559A CN1923265B (en) | 2005-06-08 | 2005-06-08 | Application of wheat germ oil in pharmaceutical preparation for preventing and treating osteoporosis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200610110559A CN1923265B (en) | 2005-06-08 | 2005-06-08 | Application of wheat germ oil in pharmaceutical preparation for preventing and treating osteoporosis |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005100782635A Division CN1302787C (en) | 2005-06-08 | 2005-06-08 | Use of several plant oils in medicinal formulation for preventing and treating osteoporosis and skin senility |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1923265A CN1923265A (en) | 2007-03-07 |
| CN1923265B true CN1923265B (en) | 2010-05-12 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200610110559A Expired - Fee Related CN1923265B (en) | 2005-06-08 | 2005-06-08 | Application of wheat germ oil in pharmaceutical preparation for preventing and treating osteoporosis |
Country Status (1)
| Country | Link |
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| CN (1) | CN1923265B (en) |
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2005
- 2005-06-08 CN CN200610110559A patent/CN1923265B/en not_active Expired - Fee Related
Non-Patent Citations (8)
| Title |
|---|
| .小麦源生理活性成分及其功能性食品的研究.食品科学25 增刊.2004,25165-169. |
| 李书国 |
| 李书国;赵文华;李雪梅;陈辉;.小麦源生理活性成分及其功能性食品的研究.食品科学25 增刊.2004,25165-169. * |
| 李雪梅 |
| 杜红霞等.二十八烷醇研究进展.粮食与油脂 6.2005,(6),13-15. |
| 杜红霞等.二十八烷醇研究进展.粮食与油脂 6.2005,(6),13-15. * |
| 赵文华 |
| 陈辉 |
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| CN1923265A (en) | 2007-03-07 |
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