CN104922488B - A kind of Chinese medicine composition for preventing and treating Parkinson's or Parkinson's motor complication and its preparation method and application - Google Patents

Abstract

The invention provides Chinese medicine composition of a kind of preventing and treating Parkinson's (Parkinson's disease PD) or Parkinson's motor complication and its preparation method and application.The Chinese medicine composition of the preventing and treating Parkinson's or Parkinson's motor complication is made up of the bulk drug of following parts by weight：100 800 parts of sealwort, 100 400 parts of the fruit of Chinese wolfberry, 100 300 parts of the root of kudzu vine, 90 200 parts of rhizoma Gastrodiae, 100 400 parts of the red sage root, 100 300 parts of Radix Codonopsis, 70 200 parts of ginkgo leaf, more preferably it is made up of above-mentioned raw materials medicine through water extraction and alcohol precipitation method.The Chinese medicine composition has significant curative effect to PD, and safe, has no toxic side effect；It is a kind of Chinese medicine composition for safely and effectively preventing Parkinson's or Parkinson's motor complication, has a good application prospect.

Description

A kind of Chinese medicine composition for preventing and treating Parkinson's or Parkinson's motor complication and its Preparation method and application

Technical field

The present invention relates to field of medicaments, more particularly to a kind of Chinese medicine for preventing and treating Parkinson's or Parkinson's motor complication Composition and its preparation method and application.

Background technology

Parkinson's (Parkinson ' s disease PD) or the cause of disease of Parkinson's motor complication are unknown so far, grind Study carefully and think that the disease is formed with heredity, environmental factor, infection, aging, oxidative stress, excessive free radical and neurotrophic factor lacks Weary grade is relevant, is the result of number of mechanisms synergy.PD patient be to tremble, muscle rigidity, motion reduce, attitudinal reflex barrier Hinder the extrapyramidal system illness being characterized, be more common in the elderly.

Treatment to PD, Western medicine continue to use the medicines such as levodopa, Madopar always, can only temporarily improve symptom, and side effect is tight Weight, and as the extension of medicine time, effective percentage are gradually reduced, curative effect is not notable or invalid after 3-5, but to related internal organs Malicious pair make progressive exacerbation.

At present, having been reported in prompting PD patient's brain tissue has the high expression of glycosylated end-product receptor (RAGE) and glycosylation The deposition of end-product (AGE).Brain tissue RAGE mRNA overexpression can excite AGE binding sites combined with RAGE after one Series of cell biological respinse (such as response to oxidative stress and the various cell factors of activation nuclear factor kappaB induction produce), causes nerve The oxidativestress damage of cell.The conditioning agent for inquiring into AGE inhibitor and RAGE mRNA expression is expected to turn into new cerebral protective agent. The present inventor's early stage, the series of base research to Siberian solomonseal rhizome polysaccharide (being content highest bioactive substance in sealwort) had revealed that, yellow Smart polysaccharide plays the role of to reduce AGE concentration in experimental animal brain tissue and suppresses RAGE mRNA expression.

However, the target spot that Siberian solomonseal rhizome polysaccharide is directed to is limited, though because PD morbidities are relevant with brain, using kidney as this, using spleen as root, Using liver as mark, therefore, multipath, Mutiple Targets are carried out using Chinese medicine composition, PD is treated at many levels and still has room for promotion.

The content of the invention

In view of the deficiencies in the prior art, the present invention passes through experimental study, there is provided one kind preventing and treating Parkinson's or Parkinson Chinese medicine composition of sick motor complication and its preparation method and application, the Chinese medicine composition have significant curative effect to PD, and It is safe, have no toxic side effect.

As described in the present invention, " preventing and treating " refers to " prevention " and/or " treatment ".

In a first aspect, prevent and treat Parkinson's or the Chinese traditional medicine composition of Parkinson's motor complication the invention discloses a kind of Thing, it is made up of the bulk drug of following parts by weight：Sealwort 100-800 parts, fruit of Chinese wolfberry 100-400 parts, root of kudzu vine 100-300 parts, rhizoma Gastrodiae 90-200 parts, red sage root 100-400 parts, Radix Codonopsis 100-300 parts, ginkgo leaf 70-200 parts.

In an embodiment of the present invention, the raw material medicines in portions by weight number is preferably：Sealwort 300-700 parts, fruit of Chinese wolfberry 200- 300 parts, root of kudzu vine 150-280 parts, rhizoma Gastrodiae 90-150 parts, red sage root 200-300 parts, Radix Codonopsis 200-250 parts, ginkgo leaf 70-180 parts.

In an embodiment of the present invention, the raw material medicines in portions by weight number is more preferably：

In an embodiment of the present invention, the raw material medicines in portions by weight number is more preferably：

In an embodiment of the present invention, the raw material medicines in portions by weight number is：

In an embodiment of the present invention, the raw material medicines in portions by weight number is：

In an embodiment of the present invention, the raw material medicines in portions by weight number is：

In an embodiment of the present invention, the raw material medicines in portions by weight number is：

In an embodiment of the present invention, the raw material medicines in portions by weight number is：

In an embodiment of the present invention, the raw material medicines in portions by weight number is：

The defects of curative effect is undesirable, Western medicine toxic side effect is larger is prevented and treated PD for existing Chinese medicine, and the present invention is through long-term anti- Multiple optimum organization formula, selects seven kinds of purification of tcm to form a kind of brand-new Chinese medicine composition, on the premise of security is ensured, There is the effect of good to PD preventing and treatings, solve the defects of existing prevention and treatment PD medicines.

Modern study discloses, and the main pathological changes of PD are nigral dopamine (DA) neuronal degeneration, and striatal dopamine contains Amount is reduced.Modern Chinese herbal medicine treatment PD dominant mechanism includes protection substantia nigra neuron cell, improves DA contents, increases brain blood flow Amount, suppress response to oxidative stress, reduce cerebrovascular neurotoxicity.The present invention is the card of " asthenia in origin and asthenia in superficiality " based on this disease, though fall ill It is relevant with brain, prevent and treat based on suitable kidney tonifying, invigorating the spleen is method, adjusts liver as side.

Sealwort, the fruit of Chinese wolfberry are monarch drug in a prescription, sealwort tonifying middle-Jiao and Qi, curing rheumatism, five viscera settling, fill Sheng muscle, strong hard marrow；Mend the exhaustion or lesion of the five internal organs Seven kinds of impairments, help muscles and bones, embellish lung.The work(that modern age Chinese pharmacology summarizes it and has reinforcing both QI and YIN is：Invigorate the spleen and benefit qi, nourshing kidney moistening lung.

Fruit of Chinese wolfberry kidney tonifying moistening lung, it is promoting production of body fluid and benefiting qi, for liver kidney kidney-KIN being deficient, the tired interior key medicine for vulcanizing benefit.Clinical pharmacology is ground Study carefully discovery, the fruit of Chinese wolfberry has good improvement cerebral ischemia, improves the effect of ischemia-reperfusion, and can improve PD rat brain line shapes The content of the neuroactive substances such as body dopamine (DA), adrenaline, serotonin, to nerve nucleus such as black substance, nucleus raphes dorsalises With good protective effect.The behavior that LBP-X can be obviously improved PD rats hinders, to the learning and remembering ability of PD rats Play good facilitation.LBP-X can also be obviously promoted the biology conjunction of the splenic lymphocytes DNA protein of ConA activation Into the restoration and reconstruction to brain tissue nerve cell play an important role.

The root of kudzu vine, rhizoma Gastrodiae are ministerial drug, the bright warp of the current foot sun of the root of kudzu vine, the effect for having rising Yang to promote the production of body fluid.Modern study proves Puerarin Entocranial artery increase cerebral blood flow (CBF) can be effectively expanded, improves vasomotion amplitude, plays good antioxidation, make intracerebral more Bar amine content is effectively lifted, and can alleviate PD patient clinical symptoms.

Rhizoma Gastrodiae, relieve dizziness, high fever, infantile convulsions, epilepsy, etc. arresting convulsion, calming the liver, helps cloudy gas, promoting blood circulation, benifit waist and knees, the work(of strengthening tendons, main return liver warp.Modern times doctor Learning research has shown that, it can increase cerebral blood flow (CBF), improves VBI, protects nerve cell, improves in corpus straitum DA contents, the loss of DA neurons is reduced, so as to improve the behavior disorder of PD rats, improve the ability of learning and memory of PD rats.

The red sage root is adjutant, its bitter color it is red enter the heart channel of Hang-Shaoyin blood system, can pass unimpeded blood vessels, tonneau Guan pulse, raise blood vessels, and it is fixed to repose The effect of will.Modern study confirms that the red sage root has and significantly improves microcirculation, reduce blood viscosity, improve cerebral tissue blood flow, promotes The survival and growth of impaired brain cell, it can be repaired for PD patient brain tissue and state of an illness rehabilitation provides good basis.

Radix Codonopsis, ginkgo leaf are to make medicine.Radix Codonopsis has bowl spares, QI invigorating, promoted the production of body fluid, blood-nourishing, the effect of strengthening vital QI to eliminate pathogenic factors.Contained is a variety of Biological active matter mass-energy increases the blood perfusion of experimental rat brain, promotes maincenter M- cholinergic recepters increase in density and acetylcholine Content increase, can improve PD patient's shaking plasy symptom, catch high brain response ability and intellectual level.

Ginkgo leaf sugariness is bitter, the thoughts of returning home, lung, large intestine channel, has promoting blood circulation and stopping pain effect.Its flavones ingredient content is very high, It can be to the experimental rabbit ear vessel retraction caused by antiadrenergic drug, and can dramatically increase experiment cat, the brain blood amount of pouring of dog, reduces brain Vascular resistence.Its extract formulation oral liquid or intravenous fluid can make the increase of PD cerebral blood flow in patients amount, improve brain nutrition, centering Pivot neurotrosis has therapeutic action.

Chinese medicine composition of the present invention is under guidance of traditional Chinese medicine theory, with reference to modern pharmacology research achievement, according to invention People's research practice experience for many years, chooses what seven kinds of bulk drugs formed according to certain weight prescription.Compatibility of the present invention is reduced the number of sternly Sincerely, diving, square medication is proper, and preventive and therapeutic effect is fairly obvious, and cure rate is high.

Second aspect, the invention discloses a kind of Chinese medicine composition for preventing and treating Parkinson's or Parkinson's motor complication Preparation method, be made of using water extraction and alcohol precipitation method by bulk drug as described in relation to the first aspect.

As described herein, " water extraction and alcohol precipitation method " is understood by row industry routine and carried out；Preferably comprise following steps：

Bulk drug is taken, adds 6-10 times to measure water, decocts 1-3 times, each 1-3 hours, receive concoction；Stand, leaching supernatant is dense Contracting, adds 2-3 times to measure alcohol (preferably 95% volumetric concentration), and (preferably precipitates overnight) is precipitated after stirring；Take precipitation, ethanol wash, Alcohol washing lotion is abandoned, obtains sediment, the i.e. Chinese medicine composition of described preventing and treating Parkinson's or Parkinson's motor complication.

In the prevention and treatment PD application of medicine is prepared, Chinese medicine composition of the present invention can routinely be made by this area Agent method is prepared into various clinically conventional formulations, for example, pharmacy can be added into the Chinese medicine composition of effective dose of the present invention Upper acceptable auxiliary material (such as disintegrant, lubricant, binder, dispersant etc.), various oral formulations are prepared into, the present invention is preferably Pulvis, decoction, tablet, granule, capsule, powder, mixture, pill, oral liquid is made；When capsule is made, every is 0.5g, it is daily 3-4 times each taking 4-10 grains, so that best results are administered at empty stomach.

To those skilled in the art, in the case where knowing active component, can according to routine formulation method, gently Easy is made various formulation products, while the auxiliary material employed in preparation is also well known in the art, such as carboxyrnethyl starch sodium, sugarcane Icing Sugar, sesame oil etc..

Third aspect present invention provides a kind of Chinese medicine preparation for preventing and treating Parkinson's or Parkinson's motor complication, by Any Chinese medicine composition and pharmaceutically acceptable auxiliary material composition as described in relation to the first aspect of effective dose.

Preferably, described effective dose is：The weight of any Chinese medicine composition as described in relation to the first aspect accounts for Chinese medicine preparation 60-80%.

Preferably, described pharmaceutically acceptable auxiliary material includes diluent and lubricant, and described diluent is selected from such as Under one or more：Starch, lactose, dextrin, microcrystalline cellulose, sucrose；Described lubricant is selected from magnesium stearate and/or micro- Powder silica gel.

Preferably, the Chinese medicine preparation is pulvis, sublimed preparation, decoction, tablet, granule, capsule, powder, mixture, ball Agent, oral liquid.

Fourth aspect present invention provides a kind of Chinese medicine composition any as described in relation to the first aspect and is preparing prevention, treatment pa Application in the medicine of the gloomy disease of gold or Parkinson's motor complication.

Beneficial effects of the present invention：The Chinese medicine composition of the present invention, its compatibility meet Chinese medicine " monarch " principle, medicine Taste number is less, has the advantages such as curative effect is high, have no toxic side effect, price is low, using Blood rheology, nourishing yin and suppressing the excessive yang method, soothing the liver is put out Wind method, rubs muscle and relieves dizziness, high fever, infantile convulsions, epilepsy, etc. method, the rules such as activating blood and removing stasis Method and dredging collateral relieve dizziness, high fever, infantile convulsions, epilepsy, etc., starts with all from cause of disease, suits the remedy to the case, to reach preventing and treating Effect；In addition, all medicine energy multipaths of monarch, Mutiple Targets in compatibility, preventing and treating Parkinson's or Parkinson at many levels Sick motor complication.Therefore, Chinese medicine composition of the invention has prevention and treatment Parkinson's or Parkinson's motion simultaneously Send out the double effectses of disease；

The preparation method of the Chinese medicine composition preparation of the present invention is simple, and cost is low, is a kind of safety suitable for long-term use The Chinese medicine composition of effectively preventing Parkinson's or Parkinson's motor complication, has a good application prospect.

Embodiment

The present invention now is further described by following examples, embodiment is only used for the purpose of illustration, does not limit this hair Bright scope, while obvious change and modification that those of ordinary skill in the art are made according to the present invention are also contained in this Within invention scope.

The preparation (1) of the Chinese medicine composition of embodiment 1

Each raw material (unit is weighed by following weight:g):

The preparation (2) of the Chinese medicine composition of embodiment 2

Each raw material (unit is weighed by following weight:g):

The preparation (3) of the Chinese medicine composition of embodiment 3

Each raw material (unit is weighed by following weight:g):

The preparation (4) of the Chinese medicine composition of embodiment 4

Each raw material (unit is weighed by following weight:g):

The preparation (5) of the Chinese medicine composition of embodiment 5

Each raw material (unit is weighed by following weight:g):

The preparation (6) of the Chinese medicine composition of embodiment 6

Each raw material (unit is weighed by following weight:g):

The preparation (7) of the Chinese medicine composition of embodiment 7

Each raw material (unit is weighed by following weight:g):

The preparation (8) of the Chinese medicine composition of embodiment 8

Each raw material (unit is weighed by following weight:g):

Embodiment 9 prepares the tablet or capsule of Chinese medicine composition of the present invention

Any described Chinese medicine compositions of Example 1-8, add 6-10 times to measure water, decoct 1-3 hours, filter out concoction. Again plus 6-10 times is measured water, is decocted 0.5-2 hours, is filtered out concoction, merge secondary decocting liquid, stand, leaching supernatant, concentration, lets cool, Add 2-3 times of concentrate to measure 95% alcohol, stir precipitates overnight.Precipitation is taken, with 0.2-2 times of 95% ethanol wash 3 times, discards alcohol Washing lotion, sediment are standby through drying；Pharmaceutical aids is added, vacuum drying crushes granulation, and tabletted or filling is encapsulated.

Manufactured capsule, every is that (Chinese medicine composition of the invention accounts for 60%-80% parts by weight to 0.5g, and auxiliary material is shallow lake Powder accounts for 20%-40% parts by weight), it is daily 3-4 times each taking 4-10 grains, so that best results are administered at empty stomach.

Embodiment 10 prepares the granule of Chinese medicine composition of the present invention

Any described Chinese medicine compositions of Example 1-8, add 6-10 times to measure water, decoct 1-3 hours, filter out concoction. Again plus 6-10 times is measured water, is decocted 0.5-2 hours, is filtered out concoction, merge secondary decocting liquid, stand, leaching supernatant, concentration, lets cool, 2-3 times of concentrate is added to measure 95% alcohol, stirring precipitation 24 hours.Taking precipitate, with 0.2-2 times of 95% ethanol wash 3 times, abandon Go alcohol washing lotion, sediment is standby through drying；Add appropriate pharmaceutical aids, pelletize, dry, whole grain, obtain particle, dispense.

Embodiment 11 prepares the mixture of Chinese medicine composition of the present invention, oral liquid

Any described Chinese medicine compositions of Example 1-8, add 6-10 times to measure water, decoct 1-3 hours, filter out concoction. Again plus 6-10 times is measured water, is decocted 0.5-2 hours, is filtered out concoction, merge secondary decocting liquid, stand, leaching supernatant, concentration, lets cool, Add 2-3 times to measure 95% alcohol, precipitated 24 hours after stirring.Precipitation is taken, with 0.2-2 times of 95% ethanol wash 3 times, alcohol is discarded and washes Liquid, sediment are standby through drying；Add appropriate pharmaceutical aids, mixture, oral liquid is made.

Contrast test sets following experimental group 1 and contrast groups 1-2

Experimental group 1, the embodiment of the present invention 5 are formulated

Add 6-10 times to measure water, decoct 1-3 hours, filter out concoction.Again plus 6-10 times is measured water, is decocted 0.5-2 hours, is filtered out medicine Juice, merge secondary decocting liquid, stand, leaching supernatant, concentration, let cool, add 2-3 times to measure 95% alcohol, precipitated 24 hours after stirring. Precipitation is taken, with 0.2-2 times of 95% ethanol wash 3 times, discards alcohol washing lotion, sediment is standby through drying, adds pure water to be configured to 180 milliliters of concoctions.Or powder is filled into the capsule of needs, or processing piece agent after drying.

Contrast groups 1, comparative example 1

With reference to the present inventor's application number：The method that the 4th section of 02102167.8 Instructions Page 2, extract the Huang in 60g sealworts Smart polysaccharide, pure water is added to be configured to 180 milliliters of concoctions.

Contrast groups 2, comparative example 2

With reference to the formula and method of the specification of patent Example 1 [0009] section of Application No. 201310074761.7：

Take " 25 grams of the Radix Astragali, 20 grams of Radix Codonopsis, 20 grams of RADIX POLYGONI MULTIFLORI PREPARATA, 15 grams of cultivated land, 15 grams of matrimony vine, 15 grams of the red sage root, grass-leaved sweetflag 15 Gram, 15 grams of root tuber of aromatic turmeric, 15 grams of radix scutellariae, 6 grams of rheum officinale ".10 taste bulk drugs are added into 800 milliliters of purified water at room temperature, soaked 6 hours, Then boil slow fire with high heat to decoct 120 minutes, about 150 milliliters of filtrate of removing slag to obtain, the dregs of a decoction add water 200 milliliters, decoct 30 points Clock, about 30 milliliters of filtrate of removing slag to obtain, about 180 milliliters of concoctions are there are, loaded in sterile glass vials, seal bottle cap is standby.

The animal experiment of Chinese medicine composition of the present invention

First, experimental method

It is prepared by 1.PD rat models：PD rat models are prepared according to the methods of Ba Mao texts.65 rats are taken to enter experiment, its In 5 be sham-operation group, in medial forebrain bundle (MFB) injecting normal saline.3% yellow Jackets anesthetized rat is injected intraperitoneally, Rat is fixed in stereo brain orienting instrument, the rat brain stereotaxic atlas with reference to written by bag new people, it is determined that right side in strict tack cranium position Medial forebrain bundle (right medical forebrain, MFB) coordinate：1. 3.7mm after bregma, sagittal suture right side 1.7mm, cranium Bone subperiosteum 7.8mm, front tooth line 2.4mm；2. 4.4mm after bregma, 1.2mm on the right side of sagittal suture, 7.8mm under cranial periosteum, front tooth Line 2.4mm.Drilled by the injection site of above-mentioned determination, extract 6-OHDA6 μ l with 10 μ l micro syringe and (given birth to containing 0.2% dimension Plain C physiological saline configuration, μ g/ μ l of concentration 4), the withdraw of the needle after injection 3 μ l, let the acupuncture needle remain at a certain point 10min, suture the surface of a wound at every.After 3 weeks, rat Apomorphine (0.5mg/kg), average speed is injected intraperitoneally>7 times/min is successful PD models.

Determined 2. prepared by model with behaviouristics：25 successful PD models are randomly divided into 4 big groups.(1) PD control groups：PD Rat model is handled using Western medicine：L-dopa methyl ester+benserazide (50mg/kg L-dopa methyl esters and 25mg/kg is injected intraperitoneally Benserazide, it is dissolved in containing in 0.2% ascorbic sterilization physiological saline), 2 times/d (at 9 points in the morning and at 5 points in afternoons), continue 4 weeks； (2) experimental group 1 (S)：Feed the concoction of the formula of experimental group 1；(3) contrast groups 1 (C1)：Feed the concoction of the formula of contrast groups 1； (4) contrast groups 2 (C2)：Feed the concoction of the formula of contrast groups 2；Fed by 9mL/kg, one time a day gavage, continue 4 weeks.

Rat behavior observation and scoring are carried out after morning medication in the 2nd, 8,15,22,29 day over the course for the treatment of.AIM Evaluation：AIM, which is divided into 4 components (upper limbs AIM, mouth face AIM, axle AIM and rotation AIM), to be evaluated, per part and basis It whether there is is divided into 5 grades (0-4) with the order of severity：0 nothing；1 duration was less than 30s；2 duration were more than 30s, were less than 60s；3 duration were more than 60s, and environmental stimuli can be allowed to stop；4 duration were more than 60s, and environmental stimuli can not be allowed to stop Only.Assessed once per 20min after medication, observe 1min every time.AIM total scores are united by the average value of total mark in observing time Meter, each component AIM maximum scores are 4 points after 1 rat a drug in theory, and total AIM scorings are 16 points.

2nd, result

Statistical analysis technique

Experimental data is analyzed using the statistical softwares of SPSS 13.0.Gained measurement data represents with mean ± standard deviation, The comparison of multiple sample averages uses one-way analysis of variance, compares examined using t two-by-two.With P<0.05 is that difference has statistics Meaning is learned, as a result as shown in table 1.

1. influence of the Chinese medicine composition to PD rat model behaviors

The influence that 1.1 Chinese medicine compositions score PD rat model each components AIM

The facial AIM scorings of mouth are prompted, average no difference of science of statistics between second day each group.(i.e. 8,15,22,29 in remaining 4 weeks My god) average significant difference (F=4.96, F=21.69, F=29.89, F=25.26P between each group<0.01)..Wherein C2 groups are Mouth face AIM scorings in 22 days reduce compared with Western medicine group, difference statistically significant (t=2.48, P<0.05), remaining time difference is equal It is not statistically significant.Remaining the internal orifice face AIM scoring in 4 weeks of C1 groups substantially reduces compared with C2 groups, the statistically significant (t=of difference 2.89, t=3.82, t=4.39, t=6.59, equal P<0.05)..Compared with S groups, S groups score C1 groups compared with C1 group mouth faces AIM Reduce, difference statistically significant (t=2.52, P<0.05).

Upper limbs AIM scorings are prompted, the 2nd, 8 day each class mean no difference of science of statistics, equal value difference between the 15th, 22,29 day each group Different statistically significant (F=15.14, F=32.93, F=35.88, P<0.01)..Wherein C2 groups upper limbs AIM scorings are less than west Medicine group, difference statistically significant (t=4.49, t=4.51, equal P<0.01)), C1 groups score than C2 group upper limbs AIM and substantially dropped It is low, difference statistically significant (t=3.46, t=4.10, t=3.98, equal P<0.01)..S groups are compared with C1 groups, and difference is without system Meter learns meaning.

Axial AIM scorings are prompted, average difference statistically significant (F=3.35, F=between the 15th, 22,29 day each group 25.12, F=36.50, P<0.01).The medication of C2 groups reduces to the 22nd, 29 day axial AIM value than Western medicine group, and difference has statistics Meaning (t=3.98, t=3.09, equal P<0.05).C1 groups are compared to the 29th day statistically significant (t=of difference with C2 groups 3.08 P<0.05).S groups significantly reduce axial AIM values, but compared with C1 groups, in the 29th day variant (t=2.86, P< 0.05), other times point no significant difference.

Rotate AIM scorings to prompt, average significant difference (F=10.55, F=11.66, F between the 8th, 15,22,29 day each group =18.96, F=61.28, P<0.01)..C2 groups are compared with Western medicine group, no significant difference.The medication of C1 groups to the 15th, 22nd, 29 days, rotation scoring reduction more obvious than Western medicine group, difference statistically significant (t=3.46, t=5.16, t=7.68, equal P <0.01)..S groups are compared with C1 groups, the 8th, 15,29 day difference obvious statistically significant (t=3.88, t=2.56, t= 3.64, equal P<0.05)..

The comparison of the experimental group of table 1. and control group group AIM scorings (Point)

Group

Mouse number (only)

Mouth face

Upper limbs

Axially

Rotation

Time (my god)

PD groups

5

1.50±0.23

1.53±0.24

2.51±0.18

2.68±0.28

2

S groups

5

1.41±0.20

1.63±0.28

2.40±0.16

2.62±0.24

2

C1 groups

5

1.48±0.17

1.58±0.28

2.52±0.23

2.74±0.34

2

C2 groups

5

1.53±0.19

1.59±0.20

2.44±0.18

2.66±0.25

2

PD groups

5

1.84±0.18

1.95±0.21

2.73±0.42

3.22±0.26

8

S groups

5

1.50±0.21

1.66±0.23

2.43±0.25

2.61±0.05

8

C1 groups

5

1.55±0.16

1.78±0.18

2.59±0.30

2.96±0.20

8

C2 groups

5

1.87±0.20

1.95±0.20

2.78±0.42

3.18±0.20

8

PD groups

5

2.14±0.21

2.20±0.18

2.95±0.30

3.38±0.29

15

S groups

5

1.34±0.12

1.53±0.23

2.39±0.21

2.60±0.16

15

C1 groups

5

1.48±0.13

1.73±0.22

2.70±0.25

2.86±0.20

15

C2 groups

5

1.86±0.16

2.10±0.13

2.73±0.38

3.09±0.26

15

PD groups

5

2.42±0.38

2.58±0.15

3.20±0.18

3.39±0.28

22

S groups

5

1.28±0.12

1.50±0.24

2.25±0.19

2.56±0.16

22

C1 groups

5

1.46±0.13

1.70±0.21

2.30±0.15

2.67±0.15

22

C2 groups

5

1.94±0.21

2.15±0.15

2.65±0.29

3.13±0.22

22

PD groups

5

2.40±0.38

2.62±0.15

3.18±0.15

3.52±0.25

29

S groups

5

1.22±0.10

1.55±0.22

2.14±0.80

2.30±0.86

29

C1 groups

5

1.36±0.23

1.68±0.19

2.36±0.18

2.58±0.16

29

C2 groups

5

2.10±0.14

2.15±0.21

2.80±0.28

3.26±0.16

29

Explanation:PD groups are Western medicine group；S groups design prescription group for inventor；C1 groups are Siberian solomonseal rhizome polysaccharide group (comparative example 1)；C2 groups are the prescription group that comparative example 2 provides.

The clinical test of the Chinese medicine composition of the present invention of embodiment 11

Preclinical phase symptom crowd (prevention group)：Only there is cacesthesia, not peaceful limb or easy phenomenon tired out in this group of crowd. Capsule (formula of embodiment 5) prepared by the embodiment of the present invention 9, each 2-4 grains of taking medicine, three times a day, is administered at empty stomach.3 months For a course for the treatment of, the 1-6 course for the treatment of is continuously taken.Symptom disappears, and does not develop into PD patient in 3 years and (reaches prevention mesh for recovery from illness ).This group of crowd totally 40, at -59 years old 42 years old age, men and women half and half, and therapeutic effect is referring to table 2；As shown in Table 2, the present invention carries In the Chinese medicine composition clinical test of confession, preventive effect is notable.

The Chinese medicine composition prevention clinical trial provided by the invention of table 2.

Clinical classical symptom crowd (treatment group)：Trembling occurs in this group of crowd, muscle rigidity, motion are reduced, attitudinal reflex The extrapyramidal symptom that obstacle is characterized, clinical definite are PD patient.Capsule prepared by the embodiment of the present invention 9 (is matched somebody with somebody by embodiment 1 Side), each 4-10 grains of taking medicine, once, it is administered at empty stomach within every 6 hours.3 months are a course for the treatment of, continuously take the 4-8 course for the treatment of.Symptom Disappear, live and take care of oneself completely, do not recurred in 1 year for recovery from illness.Symptom substantially mitigates therebetween for medication, live it is basic take care of oneself, 1 year it Interior nothing is repeatedly to be effective.Symptom mitigation is effective.Symptom is not improved to be invalid.This group of crowd PD patient totally 66, age - 76 years old 45 years old, course of disease 6-16, female 36, man 30, treat referring to table 3；As shown in Table 3, Chinese medicine provided by the invention Composition of medicine clinical testing treatment significant effect.

The Chinese medicine composition Clinical Treatment Test provided by the invention of table 3.

To further illustrate beneficial effects of the present invention, the invention provides following model case：

1st, prevention group：Mr. Wang, male, 50 years old, the course of disease 2 years.Without any inducement sense upper right acroanesthesia with myrmeciasis shouting pain before 2 years Discomfort, engender that right wrist joint is powerless and double lower limb is not trembled independently alternately.Start to be made with the embodiments of the present invention 9 Standby capsule (formula of embodiment 5), 2 tablets each time, 3 times a day, is administered at empty stomach.Taken effect after one course for the treatment of (three months), two Symptom substantially mitigates after the course for the treatment of, and symptom is wholly absent after four courses for the treatment of.Follow-up has no above-mentioned symptom resume combustion in 3 years.Avoid during medication Raw and cold diet, wine for giving off smoking, seafood, fresh-water carp, silver carp are taken, controls chamber.

2nd, treatment group：Mr. Li, female, 68 years old, brothers or lower neck trembled, muscle rigidity, limbs pain or numbness, in someone Double lower limb, which is intersected, when being walked in the case of protection advances, in " festinating gait ", binocular is inattentive, and complexion is secretly stagnant, in " mask face ", usually Subsist on swivel chair, the course of disease 10 years.With reference to other laboratory examination results, PD is diagnosed as.Start to be made with the embodiment of the present invention 9 Standby capsule (formula of embodiment 1), 6 tablets each time, every 6 hours once, and 4 times a day, a course for the treatment of symptom has mitigated, three It is clearly better after the course for the treatment of, can voluntarily slow small step walking after five courses for the treatment of.Live after eight courses for the treatment of and take care of oneself substantially.During medication Avoid and take raw and cold diet, wine for giving off smoking, seafood, fresh-water carp, silver carp, control chamber.

To sum up, Chinese medicine composition preparation of the invention is a kind of safely and effectively prevention Parkinson's or Parkinson's fortune Dynamic complication, and the Chinese medicine composition for the treatment of Parkinson's or Parkinson's motor complication motor complication, have good Application prospect.

Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should It is considered as protection scope of the present invention.

Claims (7)

1. a kind of Chinese medicine composition for preventing and treating Parkinson's or Parkinson's motor complication, it is characterised in that by following weight The bulk drug of part is made：Sealwort 100-800 parts, fruit of Chinese wolfberry 100-400 parts, root of kudzu vine 100-300 parts, rhizoma Gastrodiae 90-200 parts, the red sage root 100-400 parts, Radix Codonopsis 100-300 parts, ginkgo leaf 70-200 parts.

2. the Chinese medicine composition of preventing and treating Parkinson's or Parkinson's motor complication, its feature exist as claimed in claim 1 In being made up of the bulk drug of following parts by weight：Sealwort 300-700 parts, fruit of Chinese wolfberry 200-300 parts, root of kudzu vine 150-280 parts, rhizoma Gastrodiae 90-150 parts, red sage root 200-300 parts, Radix Codonopsis 200-250 parts, ginkgo leaf 70-180 parts.

3. the Chinese medicine composition of preventing and treating Parkinson's or Parkinson's motor complication, its feature exist as claimed in claim 1 In being made up of the bulk drug of following any parts by weight：

A kind of 4. preparation method for the Chinese medicine composition for preventing and treating Parkinson's or Parkinson's motor complication, it is characterised in that Bulk drug as claimed in claim 1 is made of water extraction and alcohol precipitation method.

5. a kind of Chinese medicine preparation for preventing and treating Parkinson's or Parkinson's motor complication, it is characterised in that by the power of effective dose Profit requires 1~3 any Chinese medicine composition and pharmaceutically acceptable auxiliary material composition.

6. Chinese medicine preparation as claimed in claim 5, it is characterised in that it is pulvis, sublimed preparation, decoction, tablet, granule, capsule Agent, powder, mixture, pill, oral liquid.

7. Chinese medicine composition is preparing prevention, treatment Parkinson's or Parkinson's motion simultaneously as described in claims 1 to 3 is any Send out the application in the medicine of disease.