CN108815342B - Traditional Chinese medicine composition for treating male infertility - Google Patents
Traditional Chinese medicine composition for treating male infertility Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/74—Rubiaceae (Madder family)
- A61K36/746—Morinda
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/60—Fish, e.g. seahorses; Fish eggs
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/62—Leeches; Worms, e.g. cestodes, tapeworms, nematodes, roundworms, earth worms, ascarids, filarias, hookworms, trichinella or taenia
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/39—Convolvulaceae (Morning-glory family), e.g. bindweed
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/43—Cuscutaceae (Dodder family), e.g. Cuscuta epithymum or greater dodder
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/60—Moraceae (Mulberry family), e.g. breadfruit or fig
- A61K36/605—Morus (mulberry)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
- A61K36/815—Lycium (desert-thorn)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/894—Dioscoreaceae (Yam family)
- A61K36/8945—Dioscorea, e.g. yam, Chinese yam or water yam
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/08—Drugs for genital or sexual disorders; Contraceptives for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis
Abstract
The invention provides a traditional Chinese medicine composition for treating male infertility, which is prepared from the following raw material medicines: 8-40 parts of morinda officinalis, 5-32 parts of wolfberry fruit, 8-40 parts of longhairy antenoron herb, 1-6 parts of leech, 8-40 parts of mulberry, 3-20 parts of yam rhizome, 5-32 parts of dodder and 8-40 parts of swim bladder. The traditional Chinese medicine composition has obvious effect of treating oligospermia.
Description
Technical Field
The invention relates to a traditional Chinese medicine composition, in particular to a traditional Chinese medicine composition for treating male infertility, and belongs to the field of medicines.
Background
With the increase of working pressure, the complexity of social relationships and the spread of venereal diseases, male infertility is increasing, and bad living habits further aggravate the phenomenon. About 15% of couples worldwide are troubled by infertility. Researches show that the semen quality of normal men in China is in a descending trend during the year of 1995-2008, and the stage is in the period of industrialized development and great economic prosperity in China. Therefore, the damage of industrial development to the environment and the change of the life habits of people due to economic growth are inferred to be one of the reasons for the reduction of the semen quality of normal men in China.
The traditional Chinese medicine considers that the pathogenesis of oligospermia and asthenospermia is mostly caused by kidney qi deficiency or kidney yin and yang dual damage, and the reasons of the oligospermia and asthenospermia are probably irregular in long-term life, too long in overload work, too frequent in sexual life to achieve desire saving and sperm retention and the like. In addition, phlegm-turbidity and blood stasis blocking the blood channel, which leads to blood vessel obstruction, are also common pathogenesis. Testis and epididymis are tissues with rich blood supply, common iliac arteries are external iliac arteries and internal iliac arteries, internal iliac arteries are internal private arteries, internal private arteries are deep penis arteries, bulbar urethra arteries and scrotum arteries, the three arteries are parts of a cardiovascular system of the whole body, have a relationship of honor and damage with heart and other blood vessels, and therefore blood obstruction caused by blood stasis and turbid phlegm at any position in the blood vessels can affect the normal blood supply of the testis and the epididymis to cause local congestion or ischemia, and finally lesion occurs.
In the aspect of treatment, the traditional Chinese medicine commonly uses methods of tonifying kidney and replenishing essence, promoting blood circulation and removing blood stasis, clearing damp-heat and the like. Chinese patent CN 104069314 a discloses a "vital essence replenishing soup" for treating male infertility, azoospermia, oligospermia and asthenospermia, which comprises raw material medicines of dried rehmannia root, cornus officinalis, Chinese yam, medlar, mulberry, dodder, polygala tenuifolia and cynomorium songaricum, codonopsis pilosula, astragalus, morinda root, desertliving cistanche and antler glue are added for patients with infertility due to essence deficiency, superior ginseng is added, and the kidney of seal dog is treated by 1:5 grinding the components into powder and mixing with the decoction for oral administration; rhizoma anemarrhenae, cortex Phellodendri, radix asparagi, and radix Ophiopogonis can be added for patients with yin deficiency, hyperactivity of fire and infertility; radix Codonopsis, radix astragali, radix Morindae officinalis, herba Epimedii, radix Aconiti lateralis Preparata, and rhizoma Cibotii can be added for patients with yang deficiency and infertility; herba Lysimachiae Christinae, Coicis semen, and pericarpium Citri Reticulatae viride, pericarpium Citri Tangerinae, herba Lycopi, semen Persicae, and Carthami flos for patients with damp-heat syndrome. The invention has the disadvantages of complex composition of raw material medicines, large dosage and no contribution to long-term administration.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides the traditional Chinese medicine composition for treating male infertility, which is simple in composition and remarkable in effect.
As one aspect of the present invention, the present invention provides a traditional Chinese medicine composition for treating male infertility, which is prepared from the following raw material medicines: morinda officinalis, fructus Lycii, herba Lysimachiae Christinae, Hirudo, Mori fructus, rhizoma Dioscoreae Septemlobae, semen Cuscutae, and air bladder Pseudosciaenae Seu Acipenser.
In a specific embodiment, the traditional Chinese medicine composition is prepared from the following raw material medicines: 8-40 parts of morinda officinalis, 5-32 parts of wolfberry fruit, 8-40 parts of longhairy antenoron herb, 1-6 parts of leech, 8-40 parts of mulberry, 3-20 parts of yam rhizome, 5-32 parts of dodder and 8-40 parts of swim bladder.
In a preferred embodiment, the traditional Chinese medicine composition is prepared from the following raw material medicines: 10-32 parts of morinda officinalis, 7-28 parts of wolfberry fruit, 10-32 parts of longhairy antenoron herb, 2-6 parts of leech, 10-32 parts of mulberry, 4-18 parts of yam rhizome, 7-28 parts of semen cuscutae and 10-32 parts of swim bladder.
In a further preferred embodiment, the traditional Chinese medicine composition is prepared from the following raw material medicines: 12-30 parts of morinda officinalis, 10-22 parts of wolfberry fruit, 12-30 parts of longhairy antenoron herb, 2-5 parts of leech, 12-30 parts of mulberry, 5-15 parts of yam rhizome, 10-22 parts of semen cuscutae and 12-30 parts of swim bladder.
In a further preferred embodiment, the traditional Chinese medicine composition is prepared from the following raw material medicines: 15-25 parts of morinda officinalis, 12-18 parts of wolfberry fruit, 15-25 parts of longhairy antenoron herb, 2-4 parts of leech, 15-25 parts of mulberry, 7-13 parts of yam rhizome, 12-18 parts of dodder and 15-25 parts of swim bladder.
In the most preferred embodiment, the Chinese medicinal composition is prepared from the following raw material medicaments: 20 parts of morinda officinalis, 15 parts of wolfberry fruit, 20 parts of desmodium, 3 parts of leech, 20 parts of mulberry, 10 parts of yam rhizome, 15 parts of dodder and 20 parts of swimming bladder;
or 17 parts of morinda officinalis, 17 parts of wolfberry fruit, 16 parts of desmodium, 4 parts of leech, 16 parts of mulberry, 12 parts of yam rhizome, 13 parts of dodder and 23 parts of swimming bladder;
or 23 parts of morinda officinalis, 13 parts of wolfberry fruit, 24 parts of desmodium, 2 parts of leech, 23 parts of mulberry, 8 parts of yam rhizome, 17 parts of semen cuscutae and 16 parts of swimming bladder.
The traditional Chinese medicine composition can be a composition formed by crushing and mixing raw material medicines, can also be an extract obtained by mixing or extracting the raw material medicines independently, or an effective part obtained by further refining and purifying the extract, and can also be a conventional preparation form prepared by adding pharmaceutically acceptable auxiliary materials.
Wherein the extraction method comprises decocting extraction, reflux extraction, immersion extraction, ultrasonic extraction, percolation extraction, microwave extraction, etc.; the purification method comprises water extraction and alcohol precipitation, alkali dissolution and acid precipitation and various column chromatography purification methods, such as a macroporous resin column, a silica gel column, a reverse phase column and the like; the conventional dosage forms include but are not limited to injections, capsules, tablets, granules, gels, sustained-release agents, oral liquids, dropping pills or nano preparations; the pharmaceutically acceptable auxiliary materials comprise: fillers, disintegrants, lubricants, suspending agents, binders, sweeteners, flavoring agents, preservatives, bases, and the like. The filler comprises: starch, pregelatinized starch, lactose, mannitol, chitin, microcrystalline cellulose, sucrose, etc.; the disintegrating agent comprises: starch, pregelatinized starch, microcrystalline cellulose, sodium carboxymethyl starch, crospolyvinylpyrrolidone, low-substituted hydroxypropylcellulose, croscarmellose sodium, etc.; the lubricant comprises: magnesium stearate, sodium lauryl sulfate, talc, silica, and the like; the suspending agent comprises: polyvinylpyrrolidone, microcrystalline cellulose, sucrose, agar, hydroxypropyl methylcellulose, and the like; the binder includes starch slurry, polyvinylpyrrolidone, hydroxypropyl methylcellulose, etc.
As another aspect of the present invention, the traditional Chinese medicine composition of the present invention can also use the extract of the raw material medicine as a raw material, so the present invention also provides a traditional Chinese medicine composition, which is prepared from the following raw materials: 8-40 parts of morinda officinalis extract, 5-32 parts of wolfberry fruit extract, 8-40 parts of desmodium extract, 1-6 parts of leech extract, 8-40 parts of mulberry extract, 3-20 parts of yam rhizome extract, 5-32 parts of semen cuscutae extract and 8-40 parts of swim bladder extract.
In a preferred embodiment, the Chinese medicinal composition is prepared from the following raw materials: 10-32 parts of morinda officinalis extract, 7-28 parts of wolfberry fruit extract, 10-32 parts of lysimachia christinae extract, 2-6 parts of leech extract, 10-32 parts of mulberry extract, 4-18 parts of yam rhizome extract, 7-28 parts of semen cuscutae extract and 10-32 parts of swim bladder extract.
In a further preferred embodiment, the Chinese medicinal composition is prepared from the following raw materials: 12-30 parts of morinda officinalis extract, 10-22 parts of wolfberry fruit extract, 12-30 parts of desmodium extract, 2-5 parts of leech extract, 12-30 parts of mulberry extract, 5-15 parts of yam rhizome extract, 10-22 parts of semen cuscutae extract and 12-30 parts of swim bladder extract.
In a further preferred embodiment, the Chinese medicinal composition is prepared from the following raw materials: 15-25 parts of morinda officinalis extract, 12-18 parts of wolfberry fruit extract, 15-25 parts of desmodium extract, 2-4 parts of leech extract, 15-25 parts of mulberry extract, 7-13 parts of yam rhizome extract, 12-18 parts of semen cuscutae extract and 15-25 parts of swim bladder extract.
In the most preferred embodiment, the Chinese medicinal composition is prepared from the following raw materials: 20 parts of morinda officinalis extract, 15 parts of wolfberry fruit extract, 20 parts of desmodium extract, 3 parts of leech extract, 20 parts of mulberry extract, 10 parts of yam rhizome extract, 15 parts of semen cuscutae extract and 20 parts of swim bladder extract;
or 17 parts of morinda officinalis extract, 17 parts of wolfberry fruit extract, 16 parts of desmodium extract, 4 parts of leech extract, 16 parts of mulberry extract, 12 parts of yam rhizome extract, 13 parts of semen cuscutae extract and 23 parts of swim bladder extract;
or 23 parts of morinda officinalis extract, 13 parts of wolfberry fruit extract, 24 parts of desmodium extract, 3 parts of leech extract, 23 parts of mulberry extract, 8 parts of yam rhizome extract, 17 parts of semen cuscutae extract and 16 parts of swim bladder extract.
As a preferred embodiment, the extracts are water extracts of the respective raw materials.
As a second aspect of the invention, the invention provides an application of the traditional Chinese medicine composition in preparing a medicine for treating male infertility. In a specific embodiment, the Chinese medicinal composition has a remarkable effect on oligoasthenospermia; more particularly, the traditional Chinese medicine composition can improve the sperm quality and the sex hormone content.
The raw material medicines of the invention, namely morinda officinalis, medlar, longhairy antenoron herb, leech, mulberry and dodder, are all recorded in Chinese pharmacopoeia (2015 edition). The rhizoma Dioscoreae Septemlobae is selected from rhizoma Dioscoreae Septemlobae and rhizoma Dioscoreae Septemlobae recorded in Chinese pharmacopoeia (2015 edition); fish maw is recorded in Zhejiang province Chinese medicine processing Specification (published by Zhejiang province health parlor, Zhejiang science and technology publishers), also called yellow croaker gelatin and line fish gelatin, and is Pseudosciaena crocea of family ShihouPseudosciaena crocea(Richardson) dried fat.
In the traditional Chinese medicine composition, the morinda officinalis is pungent and sweet in taste, slightly warm in nature and non-toxic, enters liver and kidney channels, tonifies the kidney and yang, dispels the wind-damp, strengthens muscles and bones, can tonify fire without the disadvantage of burning yin, and is really a waning medicine; the medlar is sweet in taste and neutral in nature, enters liver and kidney meridians, has mild drug property, tonifies vital essence and strengthens vagina, and the medlar and the morinda officinalis are monarch drugs which are matched with each other to coordinate yin and yang. Dodder seed, semen Cuscutae, being pungent and sweet in flavor, neutral in nature, is good at nourishing yin, and has the actions of dredging all vessels, warming and activating yang in nourishing, and is a medicine for tonifying both yin and yang; swim bladder is specialized in kidney meridian, and has effects of replenishing essence, nourishing yin, and warming sperm; mulberry fruit, fructus Mori benefits kidney to arrest spontaneous emission, tonify blood and promote fluid production; the dodder, the swim bladder and the mulberry are used together as ministerial drugs, which not only can help the morinda officinalis to tonify the kidney and strengthen yang, but also can help the medlar to replenish essence and benefit marrow. The leech is good in removing the aversion to blood and has the effects of breaking blood and removing stasis, and all blood breaking medicines are harmful to qi system, only the leech is salty in taste and is specially used for blood system, but the blood breaking is not harmful to qi system; the lysimachia christinae hance can induce diuresis to clear damp-heat, the yam rhizome can induce diuresis to expel dampness and remove turbidity, so that the pathogenic factors can be discharged from urine and the pathogenic factors can be removed from the body resistance, and the lysimachia christinae hance, the yam rhizome and the leech are used as adjuvant and auxiliary medicines. The whole formula comprehensively applies various treatment methods of tonifying yang, nourishing yin, activating blood and promoting diuresis, has precise and appropriate compatibility, has the functions of tonifying yang without assisting dryness, nourishing yin without greasy, and attacking pathogenic factors without damaging vital qi.
The pharmacological experiment result of the invention shows that the continuous administration of the tripterygium glycosides tablet can cause the sperm motility and the motor ability of rats to be obviously reduced, and after the treatment of the traditional Chinese medicine composition, the quality of the sperm of the rats can be improved, the content of serum sex hormone can be improved, the oxidative stress injury degree of epididymis can be improved, and the like. The traditional Chinese medicine composition has obvious effect of treating oligospermia.
Detailed Description
Example 1
The formula is as follows: 20g of morinda officinalis, 15g of wolfberry fruit, 20g of desmodium, 3g of leech, 20g of mulberry, 10g of yam rhizome, 15g of dodder and 20g of swimming bladder;
the preparation method comprises the following steps: decocting the above eight medicines in water twice, the first time for 2 hr and the second time for 1.5 hr, mixing decoctions, filtering, and concentrating the filtrate to obtain the final product.
Example 2
The formula is as follows: 17g of morinda officinalis, 17g of wolfberry fruit, 16g of desmodium, 4g of leech, 16g of mulberry, 12g of yam rhizome, 13g of dodder and 23g of swimming bladder;
the preparation method comprises the following steps: decocting the above eight medicines in water twice, the first time for 2 hr and the second time for 1.5 hr, mixing decoctions, filtering, and concentrating the filtrate to obtain the final product.
Example 3
The formula is as follows: 23g of morinda officinalis, 13g of wolfberry fruit, 24g of desmodium, 2g of leech, 23g of mulberry, 8g of yam rhizome, 17g of dodder and 16g of swimming bladder;
the preparation method comprises the following steps: the eight medicines are added with water and ultrasonically extracted twice, each time for 40min, the extracting solution is combined, filtered, the filtrate is concentrated, and tablet auxiliary materials are added to prepare the tablet.
Example 4
The formula is as follows: 18g of morinda officinalis, 16g of wolfberry fruit, 18g of desmodium, 3g of leech, 17g of mulberry, 11g of yam rhizome, 14g of dodder and 24g of swimming bladder;
the preparation method comprises the following steps: the eight medicines are added with water for percolation extraction, the extract is filtered, the filtrate is concentrated, and granules are prepared by adding granules auxiliary materials.
Example 5
The formula is as follows: 24g of morinda officinalis, 14g of wolfberry fruit, 23g of desmodium, 2g of leech, 22g of mulberry, 9g of yam rhizome, 16g of dodder and 17g of swimming bladder;
the preparation method comprises the following steps: the eight medicines are added with water for reflux extraction twice, the first time lasts for 2 hours, the second time lasts for 1.5 hours, decoction is combined, filtered, filtrate is concentrated, kept stand and filtered, the total amount of the filtrate is adjusted to 1000 ml, stirred evenly, packaged and sterilized, and the Chinese medicine preparation is obtained.
Example 6
The formula is as follows: 13g of morinda officinalis, 21g of wolfberry fruit, 13g of desmodium, 5g of leech, 13g of mulberry, 14g of yam rhizome, 11g of dodder and 28g of swimming bladder;
the preparation method comprises the following steps: the eight medicines are added with water and ultrasonically extracted twice, each time for 40min, the extracting solution is combined, filtered, the filtrate is concentrated, and tablet auxiliary materials are added to prepare the tablet.
Example 7
The formula is as follows: 28g of morinda officinalis, 11g of wolfberry fruit, 29g of desmodium, 3g of leech, 27g of mulberry, 6g of yam rhizome, 21g of dodder and 13g of swimming bladder;
the preparation method comprises the following steps: decocting the above eight medicines in water twice, the first time for 2 hr and the second time for 1.5 hr, mixing decoctions, filtering, concentrating the filtrate, standing, filtering, adjusting the total amount of the filtrate to 1000 ml, stirring, packaging, and sterilizing.
Example 8
The formula is as follows: 14g of morinda officinalis, 20g of wolfberry fruit, 14g of desmodium, 4g of leech, 14g of mulberry, 15g of yam rhizome, 10g of dodder and 26g of swimming bladder;
the preparation method comprises the following steps: the eight medicines are added with water and ultrasonically extracted twice, each time for 40min, the extracting solution is combined, filtered, the filtrate is concentrated, and capsule auxiliary materials are added to prepare capsules.
Example 9
The formula is as follows: 26g of morinda officinalis, 10g of wolfberry fruit, 27g of desmodium, 2g of leech, 28g of mulberry, 5g of yam rhizome, 20g of dodder and 14g of swimming bladder;
the preparation method comprises the following steps: the eight medicines are added with water and ultrasonically extracted twice, each time for 40min, the extracting solution is combined, filtered, the filtrate is concentrated, and granules are prepared by adding granules auxiliary materials.
Example 10
The formula is as follows: 11g of morinda officinalis, 27g of wolfberry fruit, 11g of desmodium, 5g of leech, 11g of mulberry, 17g of yam rhizome, 8g of dodder and 31g of swimming bladder;
the preparation method comprises the following steps: the eight medicines are added with water for reflux extraction twice, the first time lasts for 2 hours, the second time lasts for 1.5 hours, decoction is combined, filtered, filtrate is concentrated, kept stand and filtered, the total amount of the filtrate is adjusted to 1000 ml, stirred evenly, packaged and sterilized, and the Chinese medicine preparation is obtained.
Example 11
The formula is as follows: 31g of morinda officinalis, 8g of wolfberry fruit, 11g of desmodium, 3g of leech, 30g of mulberry, 5g of yam rhizome, 26g of dodder and 11g of swimming bladder;
the preparation method comprises the following steps: the eight medicines are added with water and ultrasonically extracted twice, each time for 40min, the extracting solution is combined, filtered, the filtrate is concentrated, and granules are prepared by adding granules auxiliary materials.
Example 12
The formula is as follows: morinda officinalis 9g, fructus Lycii 31g, herba Lysimachiae Christinae 9g, Hirudo 6g, Mori fructus 10g, rhizoma Dioscoreae Septemlobae 18g, semen Cuscutae 6g, and air bladder 38 g;
the preparation method comprises the following steps: decocting the above eight medicines in water twice, the first time for 2 hr and the second time for 1.5 hr, mixing decoctions, filtering, concentrating the filtrate, standing, filtering, adjusting the total amount of the filtrate to 1000 ml, stirring, packaging, and sterilizing.
Example 13
The formula is as follows: 38g of morinda officinalis, 6g of wolfberry fruit, 37g of desmodium, 1g of leech, 38g of mulberry, 4g of yam rhizome, 30g of dodder and 10g of swimming bladder;
the preparation method comprises the following steps: the eight medicines are added with water for percolation extraction, the extract is filtered, the filtrate is concentrated, and granules are prepared by adding granules auxiliary materials.
Example 14
The formula is as follows: 20g of morinda officinalis extract, 15g of wolfberry fruit extract, 20g of desmodium extract, 3g of leech extract, 20g of mulberry extract, 10g of yam rhizome extract, 15g of dodder extract and 20g of swimming bladder extract.
Example 15
The formula is as follows: 17g of morinda officinalis extract, 17g of wolfberry fruit extract, 16g of desmodium extract, 4g of leech extract, 16g of mulberry extract, 12g of yam rhizome extract, 13g of dodder extract and 23g of swimming bladder extract.
Example 16
The formula is as follows: 23g of morinda officinalis extract, 13g of wolfberry fruit extract, 24g of desmodium extract, 2g of leech extract, 23g of mulberry extract, 8g of yam rhizome extract, 17g of dodder extract and 16g of swimming bladder extract.
Example 17
The formula is as follows: 18g of morinda officinalis extract, 16g of wolfberry fruit extract, 18g of desmodium extract, 3g of leech extract, 17g of mulberry extract, 11g of yam rhizome extract, 14g of dodder extract and 24g of swimming bladder extract.
Example 18
The formula is as follows: 24g of morinda officinalis extract, 14g of wolfberry fruit extract, 23g of desmodium extract, 2g of leech extract, 22g of mulberry extract, 9g of yam rhizome extract, 16g of dodder extract and 17g of swimming bladder extract.
Example 19
The formula is as follows: 13g of morinda officinalis extract, 21g of wolfberry fruit extract, 13g of desmodium extract, 5g of leech extract, 13g of mulberry extract, 14g of yam rhizome extract, 11g of dodder extract and 28g of swimming bladder extract.
Example 20
The formula is as follows: 28g of morinda officinalis extract, 11g of wolfberry fruit extract, 29g of desmodium extract, 3g of leech extract, 27g of mulberry extract, 6g of yam rhizome extract, 21g of dodder extract and 13g of swimming bladder extract.
Example 21
The formula is as follows: 14g of morinda officinalis extract, 20g of wolfberry fruit extract, 14g of desmodium extract, 4g of leech extract, 14g of mulberry extract, 15g of yam rhizome extract, 10g of dodder extract and 26g of swimming bladder extract.
Example 22
The formula is as follows: 26g of morinda officinalis extract, 10g of wolfberry fruit extract, 27g of desmodium extract, 2g of leech extract, 28g of mulberry extract, 5g of yam rhizome extract, 20g of dodder extract and 14g of swimming bladder extract.
Example 23
The formula is as follows: 11g of morinda officinalis extract, 27g of wolfberry fruit extract, 11g of desmodium extract, 5g of leech extract, 11g of mulberry extract, 17g of yam rhizome extract, 8g of dodder extract and 31g of swimming bladder extract.
Example 24
The formula is as follows: 31g of morinda officinalis extract, 8g of wolfberry fruit extract, 11g of desmodium extract, 3g of leech extract, 30g of mulberry extract, 5g of yam rhizome extract, 26g of dodder extract and 11g of swimming bladder extract.
The extracts described in examples 14-24 above are water extracts of the respective raw materials.
Effect example 1
1 materials and methods
1.1 Experimental materials
1.1.1 Experimental animals
SPF male SD rat with weight of 200-: SCXK (Jing) 2016-.
1.1.2 Experimental drugs
Wuzi Yanzong pill: manufactured by Beijing Tongrentang GmbH pharmaceutical manufacturing, 6g (60 granules) per time, twice a day, approved article No.: the national standard of medicine Z11020188.
Tripterygium glycosides tablets: manufactured by qianjin synergy pharmaceuticals, inc, of south of the lake, 10 mg x 50, approval paper: z43020138.
The traditional Chinese medicine composition is prepared according to the method of example 1.
1.1.3 Main test reagents
Domestic medium m 199500 mL brand: invitrog. The total superoxide dismutase test box is provided by Nanjing under the condition of stock number: a001-1. The propylene glycol test box is provided by Nanjing, and has a product number of: a003-1.
1.1.4 Main Experimental instruments
Sperm motility analysis system, model Hamilton IVOS Tox, shangham specter. Desk centrifuge, model: TGL-16C. Pure water meter, model: AJC-0501-P. Miniature vortex mixer, model: WH-2. Water bath, type: TL-420D. Microplate reader, model EPOCH.
1.1.5 Experimental methods
36 male rats with the weight of 200-. Except for a normal group, the other groups are drenched with tripterygium glycosides 30 mg/(kg. d) for 40 days, a oligozoospermia model is established, distilled water is drenched into the normal group and the model group from 41 days, the positive medicine is drenched into the stomach by Wuzi Yanzong pills, each group of the traditional Chinese medicine composition is drenched into the stomach by the dosage of 3, 6 and 12 times of the equivalent dosage of human bodies, and the testis index, the sperm quality, the serum sex hormone, the cell cycle of the spermatogenic cells and the HE staining of rats are calculated after 4 weeks of continuous drenching.
1.1.6 drug dosage calculation
The high, medium and low dosage groups of the Chinese medicinal composition are calculated by 12, 6 and 3 times of the equivalent dosage of a human body respectively. The Wuzi Yanzong pill is calculated by 6 times of the equivalent dose of a human body.
2 index detection
After the last administration, the rat is fasted for 12 hours and weighed, the rat is anesthetized by intraperitoneal injection with 3% chloral hydrate, the abdominal aorta is bled, serum is separated for standby, the rat is killed, the testis epididymis tissue of the rat is separated, and relevant index detection is carried out.
2.1 sperm quality analysis
Putting epididymis tail into physiological saline (preheated in advance) at 37 ℃, cutting, standing for 1 minute, taking 100 mu l of sperm suspension, adding 1ml of M199, putting into a constant-temperature water bath kettle at 37 ℃ for 5 minutes, taking 10 mu l of sperm suspension, putting on a sperm metering plate, selecting 6 visual fields, and completing the test within 2 minutes. The main detection indexes are as follows: sperm concentration (. times.10)6The sperm quantity is contained in the epididymis per gram of epididymis tail), sperm motility (%) (the percentage of forward moving sperms (a grade + b grade) in the sample to the total number of sperms), sperm motility (%) (the percentage of moving sperms (a grade + b grade + c grade) in the sample to the total number of sperms), sperm motility parameters (average path velocity VAP, curve velocity VCL and linear velocity VSL), and sperm motility mode parameters (linear LIN, forward STR and the like), wherein the sperms are divided into 4 grades (a is good sperm motility and moves linearly, b is more active sperm, c is sperm motility retardation and is in-situ rotation, d is non-active), the sperms at the grade a are active, and a + b is effective sperm motility.
2.2 oxidative stress of epididymis and testis
Taking unilateral epididymis tissue, preparing 5% tissue slurry with 0.9% normal saline at 4 deg.C in ice water bath, centrifuging the slurry at 3000r/min for 10min, and collecting supernatant to be tested. Taking tissue homogenate to detect the levels of MDA and SOD, using a xanthine oxidase method for detecting SOD, adopting a sparse barbiturate method for detecting MDA, testing according to the steps of a kit instruction, and determining the protein content according to the method of the Coomassie brilliant blue protein determination kit provided by the kit.
MDA content (nmol/mgprot) = determination OD value-control OD value/standard OD value-blank OD value × (10 nmol/ml) standard substance concentration/protein concentration of sample to be tested (mgprot/ml).
Total SOD activity (U/mgprot) = control OD value-measured OD value/control OD value/50%. reaction system dilution factor/sample protein concentration to be measured (mgprot/ml). sample dilution factor before measurement.
3 statistical method
Data are expressed as means ± standard deviations (X ± s), and one-way analysis of variance (ANOVA) is performed using software SPSS 20.0, with significance given that p <0.05 is the difference.
4 results
4.1 Effect of the Chinese medicinal composition of the present invention on sperm quality
The results are shown in Table 1.
TABLE 1 Effect of the Chinese medicinal composition of the present invention on sperm quality (X + -s, n = 6)
Note: Δ p <0.05 vs. blank group,. sp <0.05 vs. model group
The results show that after the tripterygium glycosides gavage molding method is adopted, the sperm count, concentration and sperm motility of the rats in the model group are obviously lower than those in the normal group (p is less than 0.05), and the success of molding is prompted. After 4 weeks of continuous administration, the indexes of sperm count and concentration, namely sperm motility and the like of each dosage group of the traditional Chinese medicine composition are obviously improved (P is less than 0.05), and the effect is not obviously different from that of a positive medicine.
4.2 Effect of the Chinese medicinal composition of the present invention on sperm motility speed
The results are shown in Table 2.
TABLE 2 Effect of the Chinese medicinal composition of the present invention on sperm motility speed (X + -s, n = 6)
| Group of | Dosage form | VAP path velocity μm/s | VCL track speed μm/s | VSL precession velocity μm/s | AREA (Sport AREA) |
| Blank set (distilled water) | 3mL | 87.90±8.05 | 108.60±20.95 | 71.46±3.85 | 14.55±1.07 |
| Model set (Tripterygium glycosides) | 30mg/kg | 43.45±38.74△ | 53.63±47.91△ | 40.18±36.86△ | 8.53±6.92△ |
| Wuzi Yanzong pill group | 1.02 g/kg | 84.80±9.79* | 119.13±14.02* | 68.35±4.99* | 14.01±1.06* |
| High dosage group of Chinese medicinal composition | 21.08 g/kg | 85.71±30.35* | 101.76±23.28* | 62.75±7.63* | 12.93±2.69* |
| Medium-dosage group of traditional Chinese medicine composition | 10.54 g/kg | 83.83±10.31* | 119.60±21.22* | 67.80±5.05* | 14.63±1.35* |
| Low-dose group of traditional Chinese medicine composition | 5.27 g/kg | 75.06±19.70* | 102.11±32.89* | 64.11±12.03* | 14.43±0.98* |
Note: Δ p <0.05 compared to blank group,. sp <0.05 compared to model group.
The result shows that indexes such as the path speed, the track speed, the movement area and the like of the rat sperm in the model group are obviously lower than those in the blank group (p is less than 0.05). The traditional Chinese medicine composition obviously improves indexes such as sperm path speed, movement area and the like (p is less than 0.05), and has no obvious difference with positive medicines.
4.3 the Effect of the Chinese medicinal composition of the invention on SOD and MDA proteins in epididymis
The results are shown in Table 3.
TABLE 3 Effect of the Chinese medicinal composition of the present invention on SOD and MDA proteins in epididymis (X + -s, n = 6)
| Group of | Dosage form | Superoxide dismutase SOD | Malondialdehyde MDA |
| Blank set (distilled water) | 3mL | 8.07±0.60 | 6.38±0.80 |
| Model set (Tripterygium glycosides) | 30mg/kg | 5.78±1.59 | 9.84±1.50△ |
| Wuzi Yanzong pill group | 1.02 g/kg | 6.17±1.28 | 4.75±0.55* |
| High dosage group of Chinese medicinal composition | 21.08g/kg | 7.81±0.81 | 5.92±0.66* |
| Medium-dosage group of traditional Chinese medicine composition | 10.54 g/kg | 11.15±5.27* | 5.89±1.70* |
| Low-dose group of traditional Chinese medicine composition | 5.27 g/kg | 6.98±0.74 | 5.54±1.14* |
Note: Δ p <0.05 vs. blank group,. sp <0.05 vs. model group
The results show that Superoxide Dismutase (SOD) in the epididymis of the model group has no obvious change compared with other groups, but has a reduction trend. Compared with the blank group, the model group has obviously increased Malondialdehyde (Malondialdehyde MDA) in epididymis (p is less than 0.05), and the groups of the positive Chinese medicine and the Chinese medicine composition have obvious difference (p is less than 0.05) with the model group.
4.4 the Effect of the Chinese medicinal composition on oxidative stress SOD and MDA protein of testis
The results are shown in Table 4.
TABLE 4 Effect of the Chinese medicinal composition of the present invention on oxidative stress of testis SOD and MDA protein
| Group of | Dosage form | Superoxide dismutase SOD | Malondialdehyde MDA |
| Blank set (distilled water) | 3mL | 6.67±0.51 | 5.82±0.50 |
| Model set (Tripterygium glycosides) | 30mg/kg | 3.66±0.45△ | 10.94±2.73△ |
| Wuzi Yanzong pill group | 1.02 g/kg | 8.10±0.75* | 4.48±1.93* |
| High dosage group of Chinese medicinal composition | 21.08 g/kg | 9.08±2.12* | 3.66±0.45* |
| Medium-dosage group of traditional Chinese medicine composition | 10.54 g/kg | 10.14±2.73* | 5.58±1.40* |
| Low-dose group of traditional Chinese medicine composition | 5.27 g/kg | 6.65±2.43* | 4.00±0.97* |
Note: Δ p <0.05 vs. blank group,. sp <0.05 vs. model group
The results showed a significant decrease in SOD in the model testis compared to the blank (p < 0.05). Compared with a blank group, the MDA in the testis of the model group is obviously increased (p is less than 0.05), and compared with the model group, each group of the Chinese medicinal composition of the invention obviously improves the MDA protein content in the testis (p is less than 0.05), and the effect is not obviously different from that of a positive medicament.
The experimental results show that the traditional Chinese medicine composition can improve the quality of the sperms of rats, the content of serum sex hormones, the degree of oxidative stress injury of epididymis and the like after treatment. The traditional Chinese medicine composition has obvious effect of treating oligospermia.
Effect example 2
1 materials and methods
1.1 Experimental materials
1.1.1 Experimental animals
SPF male Kunming mice, weight 18-20g, provided by Beijing Wittingli laboratory animal technology Limited, license number: SCXK (Kyoto) 2016-. The mice freely drink water and eat, padding and feed are replaced every day, the environment is kept dry and clean, and the environmental temperature is 20-25 ℃.
1.1.2 Experimental drugs
Wuzi Yanzong pill: manufactured by Beijing Tongrentang GmbH pharmaceutical manufacturing, 6g (60 granules) per time, twice a day, approved article No.: the national standard of medicine Z11020188.
The traditional Chinese medicine composition is prepared according to the method of example 1.
Comparison drug (i): 12g of radix rehmanniae, 10g of dogwood, 30g of yam, 10g of medlar, 10g of mulberry, 10g of dodder, 10g of polygala tenuifolia and 10g of cynomorium songaricum, and the total is 102 g;
control drug 2: on the basis of the formula of the comparison medicament I, 12g of codonopsis pilosula, 12g of astragalus, 10g of morinda officinalis, 10g of cistanche, 10g of deerhorn glue and 1:5 of superior ginseng and testis et penis callorhini are added, ground into powder and mixed with soup to be taken, 3g of the powder is taken each time, 3 times a day, and 165g is calculated;
control drug (c): on the basis of the formula of the contrast medicament I, 6g of rhizoma anemarrhenae, 6g of cortex phellodendri and 10g of radix asparagi and 10g of radix ophiopogonis are added, and the total amount is 134 g;
control drug iv: adding 12g of codonopsis pilosula, 15g of astragalus, 10g of morinda officinalis, 10g of epimedium, 6g of prepared monkshood and 10g of cibotium barometz on the basis of the formula of the contrast medicament I, and totaling 165 g;
control drug fifth: adding 15g of longhairy antenoron herb and 12g of coix seeds on the basis of the formula of the contrast medicament I, and totaling 129 g;
contrast drugs: based on the formula of the contrast medicament I, 6g of green tangerine orange peel, 10g of tangerine peel, 10g of herba lycopi, 6g of peach kernel and 3g of safflower are added, and the total amount is 137 g.
The comparative medicaments are prepared according to the preparation method of the embodiment 1 by adopting a water decoction method.
1.1.3 Main test reagents
Cyclophosphamide: manufactured by Baxter Ontology GmbH, lot number: 7J 193A. Medium M199 (Life Technologies Corporation), batch No.: 1917554.
1.1.4 Experimental methods
Mice are fed adaptively for one week and are randomly divided into ten groups, namely a blank group, a model group, a traditional Chinese medicine composition group, a contrast medicine I-II group and a Wuzi Yanzong pill group. Except for the normal group, all the mice of the other groups were injected with 60 mg/kg of cyclophosphamide in the abdominal cavity for 5 days, and a oligospermia model was established. From 6d, the normal and model groups were gazed with distilled water. The traditional Chinese medicine and the contrast medicine are respectively irrigated with 6 times of equivalent dose of human body, and the Wuzi Yanzong pill group is irrigated with Wuzi Yanzong pill once a day for 2 weeks. After the last dose, the mice were fasted for 12h and sacrificed for subsequent testing.
1.1.5 drug dosage calculation
The Wuzi Yanzong pill group, the traditional Chinese medicine composition group and the contrast medicine (I-sixth) are respectively calculated according to 6 times of the equivalent dose of a human body.
2 index detection
Mouse sperm quality test including sperm concentration (x 10)6,/ml), sperm motility (%), and sperm aberration (%).
2.1 sperm quality detection
Separating the epididymis tail rapidly with surgical scissors, extracting adipose tissue on the epididymis tail, putting the epididymis tail into M199 culture medium preheated to 37 ℃ in advance, cutting the epididymis tail into pieces, and shaking gently to make sperm fully swim out. 10 μ l of the sperm suspension was placed on a sperm cell plate and 6 fields were selected to complete the test within 2 minutes.
3 statistical method
Data are expressed as means ± standard deviations (X ± s), and one-way analysis of variance (ANOVA) is performed using software SPSS 20.0, with significance given that p <0.05 is the difference.
4 results
4.1 Effect of the Chinese medicinal composition of the present invention on sperm quality
The results are shown in Table 5.
TABLE 5 Effect of different Chinese medicinal compositions on sperm quality (X + -s, n = 9)
| Group of | Sperm concentration 106/ml | Sperm motility% | Sperm motility% | Sperm teratogenesis% |
| Blank group | 5.52±0.24* | 50.19±3.88* | 60.85±3.63* | 5.42±0.50* |
| Model set | 1.89±1.22 | 16.01±1.23 | 5.24±1.40 | 61.65±6.04 |
| Wuzi Yanzong pill group | 4.74±1.79* | 40.93±2.18* | 44.86±9.02* | 7.97±0.93* |
| The Chinese medicinal composition of the invention | 4.61±0.39* | 40.451±3.04*# | 45.20±3.75* | 6.43±0.27* |
| Control drug group I | 2.71±0.52*# | 24.22±1.86*# | 10.61±1.48*# | 17.05±1.77*# |
| Control drug group 2 | 2.81±0.54*# | 20.78±2.08*# | 11.76±1.48*# | 18.54±1.61*# |
| Control drug group | 3.03±0.56*# | 25.50±5.27*# | 16.44±5.00*# | 16.55±0.87*# |
| Control drug group IV | 2.41±0.51# | 15.92±2.52# | 10.66±1.11*# | 24.69±2.29*# |
| Control drug group | 2.10±0.53# | 15.29±2.87# | 16.71±3.16*# | 15.34±3.97*# |
| Contrast medicine group | 2.23±0.78# | 15.02±0.57# | 15.03±1.97*# | 16.48±1.42*# |
Note: p <0.05 # is p <0.05 compared to the group of the Chinese medicinal composition of the present invention.
As can be seen from Table 5, the sperm concentration in the model group was significantly decreased (p < 0.05) compared to the blank group, indicating successful molding. Compared with the traditional Chinese medicine composition group and the model group, the sperm concentration of the Wuzi Yanzong pill group is obviously improved, and the difference has statistical significance (p is less than 0.05). The sperm concentration of the first, second and third groups of the contrast medicines is obviously improved (p is less than 0.05) compared with that of the model group, but is obviously lower than that of the traditional Chinese medicine composition (p is less than 0.05). The sperm concentration of the contrast medicine group IV, G and G has an ascending trend compared with the model group, but the difference has no statistical significance (p is more than 0.05).
The sperm motility of the model group is obviously reduced compared with that of the blank group (p < 0.05), which indicates that the model is successfully made. Compared with the model group, the sperm motility of the Wuzi Yanzong pill group and the traditional Chinese medicine composition group is obviously improved, and the difference has statistical significance (p is less than 0.05). The sperm motility of the first, second and third groups of the contrast medicine is obviously improved (p is less than 0.05) compared with that of the model group, but is obviously lower than that of the traditional Chinese medicine composition (p is less than 0.05). The sperm concentration of the contrast medicine group IV, G and G has an ascending trend compared with the model group, but the difference has no statistical significance (p is more than 0.05).
The sperm motility rate of the model group is obviously reduced compared with that of the blank group (p is less than 0.05), which indicates that the molding is successful. The Wuzi Yanzong pill group, the traditional Chinese medicine composition group and the contrast medicine (I-sixth) have obviously improved sperm motility rate compared with the model group, and the difference has statistical significance (p is less than 0.05). However, the sperm motility rate of the contrast medicament (i) -sixth is obviously lower than that of the traditional Chinese medicine composition (p < 0.05).
The sperm aberration rate of the model group is obviously increased compared with that of the blank group (p is less than 0.05), which indicates that the model is successfully molded. The Wuzi Yanzong pill group, the traditional Chinese medicine composition group and the contrast medicine (I-II) have obviously reduced sperm abnormality rate and have statistical significance (p is less than 0.05) compared with the model group. However, the sperm abnormality rate of the contrast medicament (i) -sixth is obviously higher than that of the traditional Chinese medicine composition (p < 0.05).
In conclusion, the traditional Chinese medicine composition and the control medicine can treat male oligospermia, can obviously improve sperm density, sperm motility and sperm motility rate, and can reduce sperm teratospermia rate. But the treatment effect of the traditional Chinese medicine composition is obviously better than that of a control medicine.
Claims (10)
1. The traditional Chinese medicine composition for treating male infertility is characterized by being prepared from the following raw material medicines: 8-40 parts of morinda officinalis, 5-32 parts of wolfberry fruit, 8-40 parts of longhairy antenoron herb, 1-6 parts of leech, 8-40 parts of mulberry, 3-20 parts of yam rhizome, 5-32 parts of dodder and 8-40 parts of swim bladder.
2. The traditional Chinese medicine composition of claim 1, wherein the traditional Chinese medicine composition is prepared from the following raw material medicines: 10-32 parts of morinda officinalis, 7-28 parts of wolfberry fruit, 10-32 parts of longhairy antenoron herb, 2-6 parts of leech, 10-32 parts of mulberry, 4-18 parts of yam rhizome, 7-28 parts of semen cuscutae and 10-32 parts of swim bladder;
or 12-30 parts of morinda officinalis, 10-22 parts of wolfberry fruit, 12-30 parts of longhairy antenoron herb, 2-5 parts of leech, 12-30 parts of mulberry, 5-15 parts of yam rhizome, 10-22 parts of dodder and 12-30 parts of swim bladder;
or 15-25 parts of morinda officinalis, 12-18 parts of wolfberry fruit, 15-25 parts of longhairy antenoron herb, 2-4 parts of leech, 15-25 parts of mulberry, 7-13 parts of yam rhizome, 12-18 parts of dodder and 15-25 parts of swim bladder.
3. The traditional Chinese medicine composition of claim 2, wherein the traditional Chinese medicine composition is prepared from the following raw material medicines: 20 parts of morinda officinalis, 15 parts of wolfberry fruit, 20 parts of desmodium, 3 parts of leech, 20 parts of mulberry, 10 parts of yam rhizome, 15 parts of dodder and 20 parts of swimming bladder;
or 17 parts of morinda officinalis, 17 parts of wolfberry fruit, 16 parts of desmodium, 4 parts of leech, 16 parts of mulberry, 12 parts of yam rhizome, 13 parts of dodder and 23 parts of swimming bladder;
or 23 parts of morinda officinalis, 13 parts of wolfberry fruit, 24 parts of desmodium, 2 parts of leech, 23 parts of mulberry, 8 parts of yam rhizome, 17 parts of semen cuscutae and 16 parts of swimming bladder.
4. The traditional Chinese medicine composition of any one of claims 1-3, wherein the traditional Chinese medicine composition is prepared by pulverizing and mixing the raw materials.
5. The traditional Chinese medicine composition of claim 4, wherein the traditional Chinese medicine composition is prepared into a conventional dosage form by adding pharmaceutically acceptable auxiliary materials.
6. A traditional Chinese medicine composition for treating male infertility is characterized by being prepared from the following raw materials: 8-40 parts of morinda officinalis extract, 5-32 parts of wolfberry fruit extract, 8-40 parts of desmodium extract, 1-6 parts of leech extract, 8-40 parts of mulberry extract, 3-20 parts of yam rhizome extract, 5-32 parts of semen cuscutae extract and 8-40 parts of swim bladder extract.
7. The traditional Chinese medicine composition of claim 6, wherein the traditional Chinese medicine composition is prepared from the following raw materials: 10-32 parts of morinda officinalis extract, 7-28 parts of wolfberry fruit extract, 10-32 parts of desmodium extract, 2-6 parts of leech extract, 10-32 parts of mulberry extract, 4-18 parts of yam rhizome extract, 7-28 parts of semen cuscutae extract and 10-32 parts of swim bladder extract;
or 12-30 parts of morinda officinalis extract, 10-22 parts of wolfberry fruit extract, 12-30 parts of lysimachia christinae extract, 2-5 parts of leech extract, 12-30 parts of mulberry extract, 5-15 parts of yam rhizome extract, 10-22 parts of semen cuscutae extract and 12-30 parts of swim bladder extract;
or 15-25 parts of morinda officinalis extract, 12-18 parts of wolfberry fruit extract, 15-25 parts of lysimachia christinae extract, 2-4 parts of leech extract, 15-25 parts of mulberry extract, 7-13 parts of yam rhizome extract, 12-18 parts of semen cuscutae extract and 15-25 parts of swim bladder extract.
8. The traditional Chinese medicine composition of claim 7, wherein the traditional Chinese medicine composition is prepared from the following raw materials: 20 parts of morinda officinalis extract, 15 parts of wolfberry fruit extract, 20 parts of desmodium extract, 3 parts of leech extract, 20 parts of mulberry extract, 10 parts of yam rhizome extract, 15 parts of semen cuscutae extract and 20 parts of swim bladder extract;
or 17 parts of morinda officinalis extract, 17 parts of wolfberry fruit extract, 16 parts of desmodium extract, 4 parts of leech extract, 16 parts of mulberry extract, 12 parts of yam rhizome extract, 13 parts of semen cuscutae extract and 23 parts of swim bladder extract;
or 23 parts of morinda officinalis extract, 13 parts of wolfberry fruit extract, 24 parts of desmodium extract, 3 parts of leech extract, 23 parts of mulberry extract, 8 parts of yam rhizome extract, 17 parts of semen cuscutae extract and 16 parts of swim bladder extract.
9. Use of a Chinese medicinal composition as claimed in any one of claims 1-3, 6-8 in the preparation of a medicament for the treatment of male infertility.
10. The use of a Chinese medicinal composition as claimed in any one of claims 1-3, 6-8 in the manufacture of a medicament for the treatment of oligospermia.
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