CN1912128A - Production method of temp-control double-circulation submerged fermentation - Google Patents

Production method of temp-control double-circulation submerged fermentation Download PDF

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Publication number
CN1912128A
CN1912128A CN 200510091210 CN200510091210A CN1912128A CN 1912128 A CN1912128 A CN 1912128A CN 200510091210 CN200510091210 CN 200510091210 CN 200510091210 A CN200510091210 A CN 200510091210A CN 1912128 A CN1912128 A CN 1912128A
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China
Prior art keywords
fermentation
temp
circulation
production method
submerged fermentation
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CN 200510091210
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Chinese (zh)
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薛瑜
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Individual
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Individual
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Priority to CN 200510091210 priority Critical patent/CN1912128A/en
Publication of CN1912128A publication Critical patent/CN1912128A/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a temperature control dual cycle submerged fermentation method which is fit for biochemical engineering fermenting production. The fermentation is processed in fermentor with over 1.5 slenderness ratio which controls fermenting liquor. The fermenting liquor is stirred by stirrer. In addition circulating pump extracts the fermenting liquor from the bottom of the fermentor, injects from the top along tangent backward stirrer rotation direction to form turbulent flow. Thus the stirring effect is good. The offered fermentation method has the advantages of controllable fermenting temperature, fast and even fermenting, high quality; and it is fit for large scale industrialization production.

Description

Production method of temp-control double-circulation submerged fermentation
The invention belongs to a kind of fermentation method for producing, be applicable to the biochemical engineering fermentative production.
General fermentation process has solid fermentation, liquid fermenting, submerged fermentation etc.Though all have many good qualities, but unsatisfactory part is arranged.
The present invention just provides a kind of ferment rapid, even, the controlled fermentation process of leavening temperature, the fermented quality height, and be fit to large-scale industrialized production.
The present invention is achieved in that fermentation carries out in the two circulating fermentation jars of temperature control.Fermentor tank is a vertical tank, and aspect ratio＞1.5 can make all sizes such as 10T, 20T, 50T, 100T according to producing needs.The fermentation base fluid pumps in the jar from opening for feed, and pump is back (surveying with stadimeter) thermometric (surveying with temperature measurer) completely.Temperature can be regulated with jar cooling zone in week.Cooling fluid is arranged in the cooling zone, cooling fluid behind refrigerator by the pump cooling that circulates.After temperature is suitable, pump into bacterium liquid co-fermentation by another opening for feed, leavening temperature is regulated by cooling system, mixes (internal recycle) by the driven by motor agitator.Fermented liquid is extracted out from the jar bottom with recycle pump in addition, tangentially anti-agitator sense of rotation is injected (outer circulation) and a jar interior liquid fusion from top, forms turbulent flow, increases mixing effect.Cooling zone is to be buckled by the small-sized channel-section steel of spiral type to be welded on the stainless steel tank body, vertically connects with the shaped steel stiffening web again, and to increase tank intensity and rigidity, tank deck has vacuum pressure valves, and there is bearing at a jar end.Between yeast phase, by discharge hole for discharge, finish a circulation from the qualified back of thief hatch sampling chemical examination.Open the cleanser cleaning after having gone out material, so that the next cycle is used again.
Tank deck is equipped with manhole, makes maintenance and uses.The jar shell has thermal insulation layer, in order to temperature control.
Below in conjunction with accompanying drawing, more detailed description is done in invention.
Enforcement of the present invention is implemented through the temp-control double-circulation submerged fermentation jar.Fermentor tank tank body 14 is by stainless steel, stadimeter 11 (survey liquid level) is equipped with at the top, temperature measurer 10 (thermometric degree), manhole 9 (the processing maintenance is used), vacuum pressure valves 8 " (reinforced; fermentation) opens venting when the jar internal pressure increases; (cooling; discharging) air inlet when the jar internal pressure reduces ", the jar internal upper part is equipped with annular cleanser 7 (being used for jar interior a cleaning), jar is supported by bearing 20, opening for feed 2 is equipped with in the bottom, opening for feed 3, discharge port 18, thief hatch 19, agitator 4 is equipped with in the bottom, drag by motor 1, the fermentation base fluid is pumped in the jar by opening for feed 3, liquid level is measured by stadimeter 11, pump is completely back by temperature measurer 10 thermometrics, pump into bacterium liquid, mixed fermentation by opening for feed 2 again after temperature is suitable.
So-called " temperature control ", promptly leavening temperature can be controlled.Tank body 14 periphery buttons are welded with the small-sized channel-section steel of spiral type as cooling zone 15, and for increase tank intensity, rigidity vertically are welded with stiffening web 13, cooling fluid is used as power with pump 17, circulation is done heat exchange in refrigerator 16 in cooling zone 15.The temperature of the size decision cooling fluid of heat exchange amplitude is in order to the temperature of control fermented liquid.
So-called " two circulation ", promptly fermenting process carries out interior outer circulation.Internal recycle drags agitator 4 by motor 1 and stirs fermented liquid, owing to be submerged fermentation, jar aspect ratio h: d＞1.5, therefore stir insufficient.Fermented liquid is extracted out from the jar bottom by recycle pump 6 in addition, injected (outer circulation) from top along contrary agitator 4 sense of rotation of tangent line, merge, form turbulent flow,, make fermentation evenly to improve mixing effect with pot liquid through pipeline 5, effective.
In the fermenting process, can be from thief hatch 19 sampling chemical examination, stop fermentation after qualified, discharge by discharge port 18.
Fermentation process provided by the invention, leavening temperature is controlled, and fermentation is rapid, even, quality is high, and is fit to large-scale industrialized production.

Claims (4)

1. production method of temp-control double-circulation submerged fermentation is characterized in that: the fermented liquid temperature control, and use " interior outer circulation " to stir, make fermentation rapidly, evenly, fermentation is carried out in the vertical tank of h:d＞1.5.
2. according to the described production method of temp-control double-circulation submerged fermentation of claim I, it is characterized in that: vertical tank is the temp-control double-circulation submerged fermentation jar.
3. according to the described production method of temp-control double-circulation submerged fermentation of claim I, it is characterized in that: the fermentor tank tank body is a stainless steel, and outside button is welded with spiral small-sized channel-section steel cooling zone, and cooling zone is reinforced with stiffening web, increase tank body rigidity and intensity, can save lot of materials and expense.
4. according to the described production method of temp-control double-circulation submerged fermentation of claim I, it is characterized in that: tank deck is equipped with thermo detector, stadimeter, respiratory organ, cleanser, the automatic routine height.
CN 200510091210 2005-08-10 2005-08-10 Production method of temp-control double-circulation submerged fermentation Pending CN1912128A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 200510091210 CN1912128A (en) 2005-08-10 2005-08-10 Production method of temp-control double-circulation submerged fermentation

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 200510091210 CN1912128A (en) 2005-08-10 2005-08-10 Production method of temp-control double-circulation submerged fermentation

Publications (1)

Publication Number Publication Date
CN1912128A true CN1912128A (en) 2007-02-14

Family

ID=37721184

Family Applications (1)

Application Number Title Priority Date Filing Date
CN 200510091210 Pending CN1912128A (en) 2005-08-10 2005-08-10 Production method of temp-control double-circulation submerged fermentation

Country Status (1)

Country Link
CN (1) CN1912128A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101285032B (en) * 2007-04-09 2012-02-22 广州市从化顺昌源绿色食品有限公司 Refrigerated cylinder special for brewing litchi wine
CN105671086A (en) * 2016-02-29 2016-06-15 商丘市饮之健生物科技有限公司 Method for preparing environment-friendly enzymes by food waste
CN106222082A (en) * 2016-07-29 2016-12-14 黄玉春 A kind of multiplex Two-way Cycle fermentation tank
CN112500213A (en) * 2020-12-09 2021-03-16 上海国茏环保科技有限公司 Fertilizer production dumper

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101285032B (en) * 2007-04-09 2012-02-22 广州市从化顺昌源绿色食品有限公司 Refrigerated cylinder special for brewing litchi wine
CN105671086A (en) * 2016-02-29 2016-06-15 商丘市饮之健生物科技有限公司 Method for preparing environment-friendly enzymes by food waste
CN106222082A (en) * 2016-07-29 2016-12-14 黄玉春 A kind of multiplex Two-way Cycle fermentation tank
CN112500213A (en) * 2020-12-09 2021-03-16 上海国茏环保科技有限公司 Fertilizer production dumper

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