CN1907916A - Recipe of peat moistening agent fermentation culture medium and culture method thereof - Google Patents
Recipe of peat moistening agent fermentation culture medium and culture method thereof Download PDFInfo
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- CN1907916A CN1907916A CN 200610052780 CN200610052780A CN1907916A CN 1907916 A CN1907916 A CN 1907916A CN 200610052780 CN200610052780 CN 200610052780 CN 200610052780 A CN200610052780 A CN 200610052780A CN 1907916 A CN1907916 A CN 1907916A
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- peat
- moistening agent
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Abstract
This invention involves a peat wetting agent fermentation medium formula for and its culture method. The wetting agent is mainly used to modify peat and improve its hydrophilicity. The formula includes: yeast extract 0.4~0.5 g/L, soybean oil 60~70 g/L, sodium nitrate 3.5~4.5 g/L. potassium dihydrogen phosphate 1.5~2.2 g/L, sodium hydrogen phosphate dehydrate 1.5~2.2 g/L. magnesium sulfate heptahdyrate 0.6~1.1g/L, ferrous sulfate heptahydrate 0.005~0.012 g/L, water 0.95 L, and aqueous solution. The biological surfactant rhamnolipid generated from fermentation process of Pseudomonas aeruginosa, Bacillus subtilis with the formula and fermentation process can be used to modify peat and enhance hydrophilicity with no pollution and non-toxicity to animal and human. And the biological surfactant can not be water-soluble and has long-term effect.
Description
Technical field
The present invention relates to a kind of prescription and cultural method thereof of peat moistening agent fermentation culture medium, it is mainly used in the improvement of peat, improves the wetting ability of peat.
Background technology
Peat has another name called the peat composed of rotten mosses, is the organism that the ground flora remains form under atmosphericoxygen and microbial process, is the combination of ground flora.The application of peat in agriculture production mainly improved the soil, and produces bio-fertilizer, fertilizer, multiple (mixing) Hefei etc., and using peat can improve Soil structure, improves nitrogen, phosphorus, potassium content, makes organism, humic acids total amount increase the fertility of activating soil.At present, peat uses in afforestation, ecological recovery field in a large number as a kind of important organic matter material.But, stoped the application of peat because exsiccant peat is difficult to soak into moisture.The peat of using not only can not play the effect that water conservation is preserved moisture, and also when arid and plant contention moisture, reduces irrigation efficiency, influences plant-growth.The contained organic matter of peat can not get degraded utilization.Abroad adding aniorfic surfactant such as Sodium dodecylbenzene sulfonate in peat as the peat manufacturer of states such as Canada makes peat become hydrophilic, but, Sodium dodecylbenzene sulfonate has multiple toxic action to the animal and human, and carcinogenic, teratogenesis, mutagenesis are arranged.And, do not have secular effect easily by water-soluble drench.
Summary of the invention
Technical problem to be solved by this invention provide a kind of ratio optimization rationally, safety non-pollution, can effectively improve the prescription of the hydrophilic peat moistening agent fermentation culture medium of peat.
Technical problem to be solved by this invention also provide a kind of technology novelty, rationally, product can improve the cultural method of prescription of wetting ability, the peat moistening agent fermentation culture medium of peat
The present invention solves the problems of the technologies described above the prescription that the technical scheme that is adopted is this peat moistening agent fermentation culture medium, fills a prescription to be: yeast extract paste 0.4～0.5g/L, soybean oil 60～70g/L, NaNO
33.5～4.5g/L, KH
2PO
41.5～2.2g/L, Na
2HPO
42H
2O 1.5～2.2g/L, MgSO
47H
2O 0.6～1.1g/L, FeSO
47H
2O0.005～0.012g/L, 0.95 liter in water, solution are the aqueous solution.
The present invention solves the problems of the technologies described above the cultural method of prescription that the technical scheme that is adopted still is this peat moistening agent fermentation culture medium: prescription recited above is mixed put into fermentor tank and cultivate, the culture condition of fermentor tank is: 36～38 ℃ of temperature, 550～650 rev/mins of mixing speed, air flow is 1.2～1.4vvm, incubation time is 24h, cultivate when the content of rhamnolipid reaches 20～30g/L and finish, the mixture that obtains is a peat moistening agent.
The present invention has the following advantages and effect: fermention medium and zymotechnique with this prescription are cultivated fermentation using bacterias such as Pseudomonas aeruginosa, subtilises, consequent rhanolipid as biosurfactant, be used for improveing peat, can improve the hydrophilicity of peat, simultaneously pollution-free, the animal and human is not had toxic action, be difficult for being had secular effect by water-soluble drench.
Embodiment
Embodiment 1:
Get yeast extract paste 0.5g, soybean oil 65g, NaNO
34.5kg, KH
2PO
42.2kg, Na
2HPO
42H
2O 1.7g, MgSO
47H
2O 1.0g, FeSO
47H
2O 0.011g, put into fermentor tank after mixing with 0.95 liter water, keep 36～38 ℃ of temperature, 550～650 rev/mins of mixing speed, air flow are 1.2～1.4vvm, through the cultivation of 24h, when the content of rhamnolipid reaches 30g/L, cultivate and finish, obtain the peat moistening agent of 1.02kg, or dehydration is condensed into 10% viscous solution.
Embodiment 2:
Get yeast extract paste 0.45g, soybean oil 62g, NaNO
33.8kg, KH
2PO
41.8kg, Na
2HPO
42H
2O 1.8g, MgSO
47H
2O 0.8g, FeSO
47H
2O 0.006g, put into fermentor tank after mixing with 0.95 liter water, keep 36～38 ℃ of temperature, 550～650 rev/mins of mixing speed, air flow are 1.2～1.4vvm, through the cultivation of 24h, when the content of rhamnolipid reaches 27g/L, cultivate and finish, obtain the peat moistening agent of 1.04kg, or dehydration is condensed into 10% viscous solution.
Embodiment 3:
Get yeast extract paste 0.41g, soybean oil 68g, NaNO
34.4kg, KH
2PO
41.5kg, Na
2HPO
42H
2O 1.9g, MgSO
47H
2O 0.9g, FeSO
47H
2O 0.012g, put into fermentor tank after mixing with 0.95 liter water, keep 36～38 ℃ of temperature, 550～650 rev/mins of mixing speed, air flow are 1.2～1.4vvm, through the cultivation of 24h, when the content of rhamnolipid reaches 25g/L, cultivate and finish, obtain the peat moistening agent of 1.08kg, or dehydration is condensed into 10% viscous solution.
Embodiment 4:
Get yeast extract paste 0.43g, soybean oil 70g, NaNO
33.9kg, KH
2PO
41.9kg, Na
2HPO
42H
2O 1.5g, MgSO
47H
2O 0.7g, FeSO
47H
2O 0.008g, put into fermentor tank after mixing with 0.95 liter water, keep 36～38 ℃ of temperature, 550～650 rev/mins of mixing speed, air flow are 1.2～1.4vvm, through the cultivation of 24h, when the content of rhamnolipid reaches 28g/L, cultivate and finish, obtain the peat moistening agent of 1.05kg, or dehydration is condensed into 10% viscous solution.
Embodiment 5:
Get yeast extract paste 0.49g, soybean oil 63g, NaNO
33.5kg, KH
2PO
42.0kg, Na
2HPO
42H
2O 2.2g, MgSO
47H
2O 1.1g, FeSO
47H
2O 0.007g, put into fermentor tank after mixing with 0.95 liter water, keep 36～38 ℃ of temperature, 550～650 rev/mins of mixing speed, air flow are 1.2～1.4vvm, through the cultivation of 24h, when the content of rhamnolipid reaches 23g/L, cultivate and finish, obtain the peat moistening agent of 1.12kg, or dehydration is condensed into 10% viscous solution.
Embodiment 6:
Get yeast extract paste 0.4g, soybean oil 69g, NaNO
34.0kg, KH
2PO
42.1kg, Na
2HPO
42H
2O 2.0g, MgSO
47H
2O 0.6g, FeSO
47H
2O 0.009g, put into fermentor tank after mixing with 0.95 liter water, keep 36～38 ℃ of temperature, 550～650 rev/mins of mixing speed, air flow are 1.2～1.4vvm, through the cultivation of 24h, when the content of rhamnolipid reaches 20g/L, cultivate and finish, obtain the peat moistening agent of 1.13kg, or dehydration is condensed into 10% viscous solution.
Embodiment 7:
Get yeast extract paste 0.42g, soybean oil 64g, NaNO
34.2kg, KH
2PO
41.7kg, Na
2HPO
42H
2O 1.6g, MgSO
47H
2O 0.75g, FeSO
47H
2O 0.005g, put into fermentor tank after mixing with 0.95 liter water, keep 36～38 ℃ of temperature, 550～650 rev/mins of mixing speed, air flow are 1.2～1.4vvm, through the cultivation of 24h, when the content of rhamnolipid reaches 29g/L, cultivate and finish, obtain the peat moistening agent of 1.01kg, or dehydration is condensed into 10% viscous solution.
Embodiment 8:
Get yeast extract paste 0.44g, soybean oil 67g, NaNO
33.7kg, KH
2PO
41.6kg, Na
2HPO
42H
2O 2.1g, MgSO
47H
2O 0.95g, FeSO
47H
2O 0.010g, put into fermentor tank after mixing with 0.95 liter water, keep 36～38 ℃ of temperature, 550～650 rev/mins of mixing speed, air flow are 1.2～1.4vvm, through the cultivation of 24h, when the content of rhamnolipid reaches 21g/L, cultivate and finish, obtain the peat moistening agent of 1.08kg, or dehydration is condensed into 10% viscous solution.
Embodiment 9:
Get yeast extract paste 0.46g, soybean oil 66g, NaNO
33.6kg, KH
2PO
42.1kg, Na
2HPO
42H
2O 1.8g, MgSO
47H
2O 1.05g, FeSO
47H
2O 0.008g, put into fermentor tank after mixing with 0.95 liter water, keep 36～38 ℃ of temperature, 550～650 rev/mins of mixing speed, air flow are 1.2～1.4vvm, through the cultivation of 24h, when the content of rhamnolipid reaches 22g/L, cultivate and finish, obtain the peat moistening agent of 1.08kg, or dehydration is condensed into 10% viscous solution.
Embodiment 10:
Get yeast extract paste 0.48g, soybean oil 61g, NaNO
34.1kg, KH
2PO
41.5kg, Na
2HPO
42H
2O 1.9g, MgSO
47H
2O 0.65g, FeSO
47H
2O 0.006g, put into fermentor tank after mixing with 0.95 liter water, keep 36～38 ℃ of temperature, 550～650 rev/mins of mixing speed, air flow are 1.2～1.4vvm, through the cultivation of 24h, when the content of rhamnolipid reaches 26g/L, cultivate and finish, obtain the peat moistening agent of 1.06kg, or dehydration is condensed into 10% viscous solution.
Embodiment 11:
Get yeast extract paste 0.47g, soybean oil 68g, NaNO
34.3kg, KH
2PO
41.9kg, Na
2HPO
42H
2O 1.7g, MgSO
47H
2O 0.85g, FeSO
47H
2O 0.011g, put into fermentor tank after mixing with 0.95 liter water, keep 36～38 ℃ of temperature, 550～650 rev/mins of mixing speed, air flow are 1.2～1.4vvm, through the cultivation of 24h, when the content of rhamnolipid reaches 28g/L, cultivate and finish, obtain the peat moistening agent of 1.07kg, or dehydration is condensed into 10% viscous solution.
Embodiment 12:
Get yeast extract paste 0.43g, soybean oil 65g, NaNO
33.8kg, KH
2PO
42.0kg, Na
2HPO
42H
2O 2.0g, MgSO
47H
2O 0.8g, FeSO
47H
2O 0.007g, put into fermentor tank after mixing with 0.95 liter water, keep 36～38 ℃ of temperature, 550～650 rev/mins of mixing speed, air flow are 1.2～1.4vvm, through the cultivation of 24h, when the content of rhamnolipid reaches 27g/L, cultivate and finish, obtain the peat moistening agent of 1.07kg, or dehydration is condensed into 10% viscous solution.
Embodiment 13:
Get yeast extract paste 0.42g, soybean oil 67g, NaNO
33.7kg, KH
2PO
41.7kg, Na
2HPO
42H
2O 1.6g, MgSO
47H
2O 0.7g, FeSO
47H
2O 0.009g, put into fermentor tank after mixing with 0.95 liter water, keep 36～38 ℃ of temperature, 550～650 rev/mins of mixing speed, air flow are 1.2～1.4vvm, through the cultivation of 24h, when the content of rhamnolipid reaches 25g/L, cultivate and finish, obtain the peat moistening agent of 1.06kg, or dehydration is condensed into 10% viscous solution.
Embodiment 14:
Get yeast extract paste 0.41g, soybean oil 63g, NaNO
34.0kg, KH
2PO
41.8kg, Na
2HPO
42H
2O 2.1g, MgSO
47H
2O 1.0g, FeSO
47H
2O 0.010g, put into fermentor tank after mixing with 0.95 liter water, keep 36～38 ℃ of temperature, 550～650 rev/mins of mixing speed, air flow are 1.2～1.4vvm, through the cultivation of 24h, when the content of rhamnolipid reaches 24g/L, cultivate and finish, obtain the peat moistening agent of 1.10kg, or dehydration is condensed into 10% viscous solution.
Example:
With 0.5kg peat moistening agent and 500kg peat, and behind the 300kg water mixing stirring 12h, the surface tension of water is reduced to 29.7mN/m by 72.75mN/m, and peat is moistening fully.
And bacteriums such as the Pseudomonas aeruginosa that keeps in peat moistening agent, subtilis can quicken peat and be decomposed into humic acid, accelerate the granuleization of soil, promote the growth of microorganism and plant.
Claims (2)
1, a kind of prescription of peat moistening agent fermentation culture medium is characterized in that: filling a prescription is: yeast extract paste 0.4～0.5g/L, soybean oil 60～70g/L, NaNO
33.5～4.5g/L, KH
2PO
41.5～2.2g/L, Na
2HPO
42H
2O1.5～2.2g/L, MgSO
47H
2O 0.6～1.1g/L, FeSO
47H
2O 0.005～0.012g/L, 0.95 liter in water, solution are the aqueous solution.
2, a kind of cultural method of prescription of peat moistening agent fermentation culture medium as claimed in claim 1, it is characterized in that: fermentor tank is put in the described prescription mixing of claim 1 cultivated, the culture condition of fermentor tank is: 36～38 ℃ of temperature, 550～650 rev/mins of mixing speed, air flow is 1.2～1.4vvm, incubation time is 24h, cultivates when the content of rhamnolipid reaches 20～30g/L and finishes, and the mixture that obtains is a peat moistening agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610052780 CN1907916A (en) | 2006-08-03 | 2006-08-03 | Recipe of peat moistening agent fermentation culture medium and culture method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610052780 CN1907916A (en) | 2006-08-03 | 2006-08-03 | Recipe of peat moistening agent fermentation culture medium and culture method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1907916A true CN1907916A (en) | 2007-02-07 |
Family
ID=37699173
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200610052780 Pending CN1907916A (en) | 2006-08-03 | 2006-08-03 | Recipe of peat moistening agent fermentation culture medium and culture method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9884883B2 (en) | 2015-01-12 | 2018-02-06 | Logos Technologies, Llc | Production of rhamnolipid compositions |
| US10829507B2 (en) | 2017-02-06 | 2020-11-10 | Stepan Company | Decolorization of concentrated rhamnolipid composition |
-
2006
- 2006-08-03 CN CN 200610052780 patent/CN1907916A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9884883B2 (en) | 2015-01-12 | 2018-02-06 | Logos Technologies, Llc | Production of rhamnolipid compositions |
| US10829507B2 (en) | 2017-02-06 | 2020-11-10 | Stepan Company | Decolorization of concentrated rhamnolipid composition |
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