CN1884487A - Culture medium for promoting Lisianthus adventitious bud rooting - Google Patents
Culture medium for promoting Lisianthus adventitious bud rooting Download PDFInfo
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- CN1884487A CN1884487A CN 200610014458 CN200610014458A CN1884487A CN 1884487 A CN1884487 A CN 1884487A CN 200610014458 CN200610014458 CN 200610014458 CN 200610014458 A CN200610014458 A CN 200610014458A CN 1884487 A CN1884487 A CN 1884487A
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- lisianthus
- substratum
- adventitious bud
- bud rooting
- root
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Abstract
The invention discloses a medium which can improve the Eustoma russellianum adventitious bud to produce root, which is made up with the following components: 1 liter of MS medium, 1 liter of water and 0.4-0.6mg of heteroauxin, and using a medium which can improve the Eustoma russellianum adventitious bud to produce root can improve the Eustoma russellianum propagating coefficient and reduce the cost of manufacture of seeds, which lays the theoretical foundation of mass industrial production.
Description
Technical field
The present invention relates to a kind of substratum, particularly relate to a kind of substratum that promotes Lisianthus adventitious bud rooting.
Background technology
Lisianthus (Eustoma grandiflorum Shinn) has another name called prairie gentian, Gentianaceae Gentiana ornamental plant.Its plant is charming colourful, and flower shape uniqueness is lovely, and the pattern elegance is lucid and lively, is one of present potted flower all the fashion in the world and cut-flower kind.In order to breed Lisianthus rapidly, can adopt the method for Lisianthus external regeneration, but finding the substratum that more effectively promotes the Lisianthus external regeneration is the technical barrier that needs to be resolved hurrily.
Summary of the invention
The purpose of this invention is to provide a kind of promotion Lisianthus adventitious bud rooting substratum.
Technical scheme of the present invention is summarized as follows:
A kind of substratum that promotes Lisianthus adventitious bud rooting is made of following component:
1 liter of MS substratum, 1 liter in water and indolylacetic acid 0.4～0.6mg.
Described indolylacetic acid is preferably 0.5mg.
A kind of substratum that promotes Lisianthus adventitious bud rooting of the present invention can improve the Lisianthus breeding coefficient, has reduced the production cost of seedling, for theoretical basis has been established in its large-scale batch production production.
Embodiment
The present invention is further illustrated below in conjunction with specific embodiment.
Embodiment 1
A kind of substratum that promotes Lisianthus adventitious bud rooting adds the indolylacetic acid that 1 premium on currency adds 0.5mg again and makes in 1 liter of MS substratum.
The basic salt culture medium of MS (Marashige and Skoog Basal Salt Mixture).
MS substratum preparation: when 1 liter of (1000 milliliters) MS substratum of preparation, add ammonium nitrate 1.65 grams, saltpetre 1.9 grams, calcium chloride 0.44 gram, sal epsom 0.37 gram, potassium primary phosphate 0.17 gram, 0.83 milligram of potassiumiodide, 5.2 milligrams of boric acid, 22.3 milligrams in sal epsom, 3.6 milligrams in zinc sulfate, 0.25 milligram of Sodium orthomolybdate, 0.025 milligram in copper sulfate, 0.025 milligram of cobalt chloride, 27.8 milligrams in ferric sulfate, sucrose 30 grams and agar 7 grams.
Embodiment 2
A kind of substratum that promotes Lisianthus adventitious bud rooting adds the indolylacetic acid that 1 premium on currency adds 0.4mg again and makes in 1 liter of MS.
Embodiment 3
A kind of substratum that promotes Lisianthus adventitious bud rooting adds the indolylacetic acid that 1 premium on currency adds 0.6mg again and makes in 1 liter of MS.
Experiment
This experiment is a material with Lisianthus Deep Blue kind blade, take the design of two factor completely randoms, emphasis inquired into the MS substratum that contains indolylacetic acid to Lisianthus blade callus induction adventitious bud rooting influence, systematically having carried out with the blade is explant induction seedling Study on Regeneration, be intended to for the Lisianthus fast breeding technique provides theoretical foundation, also lay the foundation simultaneously for next step genetic transformation of carrying out Lisianthus.
1 materials and methods
1.1 material
1.1.1 vegetable material
In MS+6-BA (6-benzyladenine) substratum (0.5mg/ml), sprout the acquisition aseptic seedling by Lisianthus (Eustoma grandiflorum Shinn) seed, cutting aseptic seedling changes in the MS substratum of no hormone, to eliminate the influence of hormone in the former substratum, when seedling grows 5-6 sheet leaf, tender leaf is cut into the fritter of 0.5 * 0.5cm as experiment material.
1.1.2 substratum and hormone
The basic salt culture medium of MS (Marashige and Skoog Basal Salt Mixture), IBA (indole-3-butyricacid) indolylacetic acid.
Present technique has been found out one and has been overlapped the hormone prescription that can effectively promote the Lisianthus external regeneration, has improved breeding coefficient, has reduced the production cost of seedling, for theoretical basis has been established in its large-scale batch production production.
The adventitious bud rooting substratum is 1/2MS+IBA0.4～0.6mg/L.
1.2 method
1.2.1 the culture condition minimum medium is the MS substratum, sucrose 3%, agar powder 0.8%, 25 ± 2 ℃ of temperature, intensity of illumination 3000Lx, light application time 16h/d.
1.2.2 growth hormone is to the influence of adventitious bud rooting
With 1/2MS is minimum medium, and the NAA or the IBA that add different concns respectively induce Lisianthus adventitious bud rooting, the results are shown in Table 1.
Table 1 NAA (naphthylacetic acid) and the influence of IBA to Lisianthus adventitious bud rooting
Table 5 Effects of NAA and IBA on root regeneration
| Growth hormone hormone | Concentration (mg/L) concentration | Root of hair rate (%) Rooting rate | Mean elements Average no.of roots per shoot | Callus situation Callous growing viability |
| No NAA IBA | 0 0 0.1 0 0.15 0 0.2 0 0 0.5 0 1.0 0 1.5 | 30 60 60 70 90 80 70 | 1.2 4.4 5.0 5.6 16.6 8.2 6.5 | Not having has a small amount of callus, faint yellow callus is more, faint yellow callus is more, faint yellow nothing has a small amount of callus, faint yellow have a small amount of callus, faint yellow |
As can be seen from Table 1, in not adding the 1/2MS substratum of hormone, also can take root, but ratio is low, radical is few.In the substratum that adds NAA or IBA, root of hair rate and radical all increase, but its mode of taking root is different.In the substratum that adds NAA, at first the incision in bastem portion produces faint yellow callus, just sends out roots from callus subsequently.During transplanting, this root of being born on the callus is easy to come off from the incision of bastem portion together with callus.And root of hair rate and radical are for the highest when IBA lower concentration (0.5mg/L), and root directly bears from the indefinite bud base portion, and is more sturdy, and part has lateral root, is best root media.Find simultaneously also can produce a spot of callus along with the rising indefinite bud base portion of IBA concentration.
1.3. the transplanting of regrowth
Observe the take root situation of indefinite bud in substratum, the main root length of most of indefinite bud reaches 4-5cm in the time of 45 days, and is thicker, on it give birth to a large amount of root hairs.This moment, spacious bottle hardening was 5 days, take out regrowth then and clean the root substratum, be transplanted in 1: 1 the flowerpot of vegetable mould and vermiculite, water permeable, coating film is preserved moisture and is sprayed a water (will leave the hole on the film) every day to keep atmospheric moisture in film in one week, and temperature keeps about 25 ℃.Check after 20 days to survive situation, surviving rate reaches 86.7%.
Present technique has been found out a kind of substratum that promotes Lisianthus adventitious bud rooting, has improved breeding coefficient, has reduced the production cost of seedling, for theoretical basis has been established in its large-scale batch production production.
Claims (2)
1. substratum that promotes Lisianthus adventitious bud rooting is characterized in that being made of following component:
1 liter of MS substratum, 1 liter in water and indolylacetic acid 0.4～0.6mg.
2. a kind of substratum that promotes Lisianthus adventitious bud rooting according to claim 1 is characterized in that described indolylacetic acid is 0.5mg.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610014458 CN1884487A (en) | 2006-06-27 | 2006-06-27 | Culture medium for promoting Lisianthus adventitious bud rooting |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610014458 CN1884487A (en) | 2006-06-27 | 2006-06-27 | Culture medium for promoting Lisianthus adventitious bud rooting |
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| CN1884487A true CN1884487A (en) | 2006-12-27 |
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| CN 200610014458 Pending CN1884487A (en) | 2006-06-27 | 2006-06-27 | Culture medium for promoting Lisianthus adventitious bud rooting |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101836587A (en) * | 2010-03-29 | 2010-09-22 | 云南省农业科学院花卉研究所 | Method for obtaining eustoma regeneration plant by anther culture |
| CN103026969A (en) * | 2013-01-12 | 2013-04-10 | 云南农业大学 | Method for obtaining haplobiont through inducing sporidia of eustoma grandiflorum |
| CN105265313A (en) * | 2015-10-13 | 2016-01-27 | 湖北大学 | Eustoma russellianum seedling cultivation method |
-
2006
- 2006-06-27 CN CN 200610014458 patent/CN1884487A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101836587A (en) * | 2010-03-29 | 2010-09-22 | 云南省农业科学院花卉研究所 | Method for obtaining eustoma regeneration plant by anther culture |
| CN103026969A (en) * | 2013-01-12 | 2013-04-10 | 云南农业大学 | Method for obtaining haplobiont through inducing sporidia of eustoma grandiflorum |
| CN105265313A (en) * | 2015-10-13 | 2016-01-27 | 湖北大学 | Eustoma russellianum seedling cultivation method |
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