CN1871919A - Slow release biological agent for killing larvae of midge, preparation method and application method - Google Patents
Slow release biological agent for killing larvae of midge, preparation method and application method Download PDFInfo
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- CN1871919A CN1871919A CN 200510035119 CN200510035119A CN1871919A CN 1871919 A CN1871919 A CN 1871919A CN 200510035119 CN200510035119 CN 200510035119 CN 200510035119 A CN200510035119 A CN 200510035119A CN 1871919 A CN1871919 A CN 1871919A
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Abstract
A slow-releasing biologic product for killing the midge larvae in water source, water region and waterworks is prepared from the Israeli subset of Bacillus thuringiensis (B.t.i), sodium alginate as carrier and cross-linking agent through fermenting and reproduction to obtain B.t.i powder, dissolving others, mixing and cross-linking reaction.
Description
Technical field
The present invention relates to the Prevention Technique that a kind of water pollutes, be specifically related to a kind of preparation method and application method thereof of killing the chironomus larvas slow release biological agent, mainly be applicable to waters such as comprising surface water, landscape water, Drinking Water water head site, drinking water treatment technical process water.
Background technology
At contaminated surface water, landscape water, source water and in the water system of polluted water body as the water source, grow midge easily, chironomus larvas takes on a red color, and is commonly called as " red worm ".Though midge is the Dulicidae insect that does not sting the people, and many-sided harm is also arranged.The chironomus larvas that grows in the water supply process can be caused strong stimulus to the sense organ to user's sense organ, though also do not proved widely at present, but about midge carry pathogen achievement in research the time report arranged, drinking water safety there is potential harm, has caused the extensive concern of water supply industry; Since the midge adult in the morning with the characteristic of nuptial flight in groups at dusk, the midge that grows in a large number in surface water and the landscape water give near resident especially view place visitor cause serious puzzlement, relevant report is common in space in a newspaper.For the control of midge, at present natural water adopts insecticide to kill the method for adult more, but according to the ecological characteristic of midge, this method can not play the effect that effects a permanent cure; And in water system, generally adopt chemosterilant to kill or control according to actual conditions, but the disinfectant of excessive concentrations is easy to generate the carcinogenicity disinfection byproduct (DBP), and long-term use can make midge develop immunity to drugs.Biocidal is an optional approach for this reason, at application number is " 03113555.2 ", name is called in the application for a patent for invention of " application of the preparation method of kill mosquito preparation and kill mosquito preparation ", the bacillus thuringiensis bacterial strain Israel subclass that discloses available high virulence (is called for short B.t.i, this bacterial classification market is on sale, production unit has the Hubei agricultural pharmaceutcal corporation, Ltd of Kang Xin etc.) thalline kills chironomus larvas, and preparation method's and methods for using them of several formulations disclosed, as described fermentation processing and prepared the method for liquor and pulvis, the gemma of bacillus thuringiensis subsp israelensis and the content of parasporal crystal are not less than 10 in every milliliter of the B.t.i liquid preparation that obtains
9Individual/ml, the B.t.i pulvis is not higher than 0.2mg/L to the half lethal concentration of red worm in the III length of time.Yet in use, described various preparation lasting effects are low, all need frequently to drop into water body, could guarantee to kill the concentration of the active component of chironomus larvas; Simultaneously, because B.t.i degrades rapidly, cause very big waste in natural water.
Summary of the invention
One of the technical problem to be solved in the present invention is, above-mentioned deficiency at prior art, a kind of slow release biological agent of killing chironomus larvas is provided, and said preparation can be killed the active component of chironomus larvas to long-term the release equably of water body, thereby chironomus larvas is reached continual and steady killing effect; Said preparation is nontoxic to human body, aquatile and ecotope.
Two of the technical problem to be solved in the present invention is that the preparation method of above-mentioned slow release biological agent is provided.
Three of the technical problem to be solved in the present invention is provide the application method of above-mentioned slow release biological agent, thereby economical and effective to be killed chironomus larvas safely.
One of the technical solution used in the present invention is, a kind of slow release biological agent of killing chironomus larvas, comprise active component, carrier and crosslinking agent, it is characterized in that, described active component comprises the thalline of bacillus thuringiensis subsp israelensis, described carrier comprises sodium alginate, and described crosslinking agent comprises calcium chloride.Described active component comprises the gemma and the parasporal crystal of bacillus thuringiensis subsp israelensis.
Above-mentioned slow release biological agent, described active component is the pulvis (being called for short the B.t.i pulvis) that bacillus thuringiensis subsp israelensis makes through fermentation, described pulvis is lower than 0.2mg/L to the half lethal concentration of midge III instar larvae, and pulvis is 1 to 1～2 with the quality proportioning of sodium alginate.
Two of the technical solution used in the present invention is that a kind of preparation method who kills the slow release biological agent of chironomus larvas is characterized in that, comprises the steps:
S101: the preparation of active component, bacillus thuringiensis subsp israelensis is fermented, plate compression, atomized drying makes pulvis;
S102: the carrier sodium alginate is mixed with solution, standby behind the autoclave sterilization, step S101 gained pulvis is disperseed with sterile water earlier, join then in the solution of sodium alginate and mix;
S103: crosslinking agent calcium chloride and step S101 gained pulvis are mixed with solution with sterile water respectively, mix standbyly, its objective is to prevent that in cross-linking process the B.t.i composition spreads among the S102 in crosslinking agent;
S104: step S102 gained sodium alginate/pulvis mixed liquor is added in step S103 gained calcium chloride/pulvis mixed liquor, and placement makes crosslinked abundant, filters after solidifying and obtains the described slow release biological agent of killing chironomus larvas.
Above-mentioned preparation method, step S101 gained pulvis is lower than 0.2mg/L to the half lethal concentration of midge III instar larvae; In described step S102, it is 2～4% solution that the carrier sodium alginate is mixed with concentration, pressure cooker sterilization back is standby, it is 1～4% solution that step S101 gained pulvis sterile water is mixed with concentration, join in the solution of described sodium alginate according to 1: 1 volume ratio then, mechanical mixture is even; In described step S103, with crosslinking agent calcium chloride and step S101 gained pulvis respectively with 1～3% and the concentration of 1-4% be mixed with mixed liquor with sterile water, mix standby; In described step S104, step S102 gained sodium alginate/pulvis mixed liquor is added in step S103 gained calcium chloride/pulvis mixed liquor with the speed of become dripping, to place the crosslinked time to be at least 30 minutes, gained preparation form is a milky white granules.
Above-mentioned preparation method, in described step S102, it is 2% solution that the carrier sodium alginate is mixed with concentration, pressure cooker sterilization back is standby, it is 1% solution that step S101 gained pulvis sterile water is mixed with concentration, join in the solution of described sodium alginate according to 1: 1 volume ratio then, mechanical mixture is even; In described step S103, crosslinking agent calcium chloride and step S101 gained pulvis are mixed with mixed liquor with 2% and 1% concentration with sterile water respectively, mix standby; In described step S104, placing the crosslinked time is 60 minutes, gained preparation form is a milky white granules, by adjusting the size that step S102 gained sodium alginate/pulvis mixed liquor is added drop in step S103 gained calcium chloride/pulvis mixed liquor, the diameter that makes described preparation granules is 0.2～0.4mm, adjust the drop size and can obtain the different-grain diameter particle, satisfy the different application environment.
Three of the technical solution used in the present invention is, kill the application method of the slow release biological agent of chironomus larvas, it is characterized in that in the applying environment in waters, water source, the usage amount of described slow release biological agent reaches 500～1000mg/m for making B.t.i pulvis active ingredient
2, be preferably 600～700mg/m
2In the applying environment of water factory's structure, the usage amount of described slow release biological agent reaches 0.5～1.0mg/L for making B.t.i pulvis active ingredient, is preferably 0.6～0.7mg/L.
Technique effect of the present invention is, gained preparation of the present invention, be with nontoxic, harmless to human body, the B.t.i of natural decomposition is an active ingredient in environment, can be used for the sodium alginate that food adds is carrier, can kill the active component of chironomus larvas to long-term the release equably of water body, thereby chironomus larvas is reached continual and steady killing effect; The said preparation safety non-toxic is pollution-free, said preparation is nontoxic to human body, aquatile and ecotope, the waters such as technical process water that can be widely used in surface water, landscape water, Drinking Water water head site, drinking water treatment, especially metastable waters.
Embodiment
Embodiment one
A kind of slow release biological agent of killing chironomus larvas, comprise active component, carrier and crosslinking agent, described active component comprises the thalline of bacillus thuringiensis subsp israelensis, and thalline comprises gemma and parasporal crystal, described carrier comprises sodium alginate, and described crosslinking agent comprises calcium chloride; The active component thalline is the pulvis (being called for short the B.t.i pulvis) that is made through fermentation by bacillus thuringiensis subsp israelensis, described pulvis is not higher than 0.2mg/L to 24 hours half lethal concentrations of midge III instar larvae, and pulvis is 1 to 1 with the quality proportioning of sodium alginate.
Above-mentioned pulvis can also be 1 to 1.5 or 1 to 2 with the quality proportioning of sodium alginate.
Embodiment two
A kind of preparation method who kills the slow release biological agent of chironomus larvas is characterized in that, comprises the steps:
S101: the preparation of active component, bacillus thuringiensis subsp israelensis is fermented, plate compression, atomized drying makes pulvis, and the gained pulvis is lower than 0.2mg/L to the half lethal concentration of midge III instar larvae;
S102: it is 2% solution that the carrier sodium alginate is mixed with concentration, standby behind the autoclave sterilization, it is 1% solution that step S101 gained pulvis is mixed with concentration with sterile water, joins in the solution of described sodium alginate according to 1: 1 volume ratio then, and mechanical mixture is even; (in this step, also the carrier sodium alginate can be mixed with concentration and be 3% or 4% solution, it is 2%, 3% or 4% solution that step S101 gained pulvis is mixed with concentration with sterile water; )
S103: crosslinking agent calcium chloride and step S101 gained pulvis are mixed with mixed liquor with 2% and 1% concentration with sterile water respectively, mix standby; (in this step, in described calcium chloride and the pulvis mixed liquor, the concentration of calcium chloride can also be 1% or 3%, and the concentration of pulvis can also be 2%, 3% or 4%; )
S104: step S102 gained sodium alginate/pulvis mixed liquor is added in step S103 gained calcium chloride/pulvis mixed liquor, place made in 30 to 60 minutes crosslinked fully, gained preparation form is a milky white granules, B.t.i content is 0.5%, by adjusting the size that step S102 gained sodium alginate/pulvis mixed liquor is added drop in step S103 gained calcium chloride/pulvis mixed liquor, the diameter that makes described preparation granules is 0.2～0.4mm, adjust the drop size and can obtain the different-grain diameter particle, satisfy the different application environment.
Embodiment three
Kill the application method of the slow release biological agent of chironomus larvas, in the applying environment in waters, water source, the usage amount of embodiment one described slow release biological agent reaches 700mg/m for making B.t.i pulvis active ingredient
2
Also can make above-mentioned B.t.i pulvis active ingredient reach 500,600,800 or 1000mg/m
2
Embodiment four
Kill the application method of the slow release biological agent of chironomus larvas, in the applying environment of water factory's structure, the usage amount of embodiment one described slow release biological agent reaches 0.7mg/L for making B.t.i pulvis active ingredient.Also can as possible stopping the pond time, add suitable concentration, make above-mentioned B.t.i pulvis active ingredient reach 0.5,0.6 or 1.0mg/L according to water factory's service condition.To guarantee that chironomus larvas is in the lethality that reaches between infection period more than 80%.
Embodiment one described preparation in the water of 2% content, in 15 days dynamically the gemma release concentration greater than 1 * 10
9Individual/mL, to 24 hours lethality rates of red worm in 3 length of times greater than 80%; Preparation is dried at normal temperatures, can stablize and preserve more than 1 year.The polluted water body that said preparation is suitable for comprises: red worms such as landscape water, drinking water source ground, drinking water treatment structure multiply the breeding place, drinking water treatment can be used the go out process section of red worm of this sustained release preparation and be comprised: water factory's water inlet, sedimentation basin inlet, filter tank inlet are particularly suitable for the red worm prevention of biological activated carbon filter and kill.
In using embodiment one described preparation process, specifically can operate by the following method:
1, this sustained release preparation now prepares, and shows result of use the best under wet condition;
2, under the situation of unconditional in situ preparation, should earlier dry preparation be activated 36-48 hour with an amount of water, make it to use after the abundant swelling;
3,, should determine amount of application according to the flowability of water surface area and the depth of water and water for lake water, landscape water and water head site water.Generally according to being equivalent to 1g/m
3B.t.i pulvis or 200g/m
3The amount of wet sustained release preparation evenly spreads preparation to the water surface, because the proportion of preparation is heavier than water, sinks under water rapidly.In the waters more than 5 meters, dosage can suitably reduce for the depth of water.Used once in 15-20 breeding period days red worm.
4, at the inlet of sedimentation basin and other structure, available filter screen fixedly behind the preparation, makes water continue to change preparation by the filter screen of preparation is housed in 15-20 breeding period days red worm.
5, the active carbon biofilter is because backwashing time is long, and biomembrane provides sufficient food source for red worm and the ideal that becomes red worm is probably bred the place, in order to guarantee growth of microorganism on the biomembrane, can not use chlorine or other to kill medicament.On the active carbon upper strata, thickness is at 5-8cm with the even spreading of this sustained release preparation.According to active carbon pond water outlet gemma concentration determination result, suitably replenish after each backwash.
The B.t.i gemma is easily by disinfectant deactivations such as chlorine, chlorine dioxide, when being used for the red worm control of drinking water treatment factory, exceeds standard for fear of the output water total number of bacteria, should guarantee in contact under the residual chlorine concentration of 1mg/L more than 30 minutes.
Claims (10)
1, a kind of slow release biological agent of killing chironomus larvas, comprise active component, carrier and crosslinking agent, it is characterized in that described active component comprises the thalline of bacillus thuringiensis subsp israelensis, described carrier comprises sodium alginate, and described crosslinking agent comprises calcium chloride.
According to the described slow release biological agent of claim 1, it is characterized in that 2, described active component comprises the gemma and the parasporal crystal of bacillus thuringiensis subsp israelensis.
3, according to the described slow release biological agent of claim 1, it is characterized in that, described active component is the pulvis (being called for short the B.t.i pulvis) that bacillus thuringiensis subsp israelensis makes through fermentation, described pulvis is not higher than 0.2mg/L to 24 hours half lethal concentrations of midge III instar larvae, and pulvis is 1 to 1～2 with the quality proportioning of sodium alginate.
4, a kind of preparation method who kills the slow release biological agent of chironomus larvas is characterized in that, comprises the steps:
S101: the preparation of active component, bacillus thuringiensis subsp israelensis is fermented, plate compression, atomized drying makes pulvis;
S102: the carrier sodium alginate is mixed with solution, and pressure cooker sterilization back is standby, and step S101 gained pulvis is disperseed with sterile water earlier, joins then in the solution of sodium alginate to mix;
S103: crosslinking agent calcium chloride and step S101 gained pulvis are mixed with solution with sterile water respectively, mix standby;
S104: step S102 gained sodium alginate/pulvis mixed liquor is added in step S103 gained calcium chloride/pulvis mixed liquor, and placement makes crosslinked abundant, filters after solidifying and obtains the described slow release biological agent of killing chironomus larvas.
5, according to the described preparation method of claim 4, it is characterized in that:
Step S101 gained pulvis is lower than 0.2mg/L to the half lethal concentration of midge III instar larvae;
In described step S102, it is 2～4% solution that the carrier sodium alginate is mixed with concentration, and the sterilization back is standby, and it is 1～4% solution that step S101 gained pulvis sterile water is mixed with concentration, join in the solution of described sodium alginate according to 1: 1 volume ratio then, mechanical mixture is even;
In described step S103, with crosslinking agent calcium chloride and step S101 gained pulvis respectively with 1～3% and the concentration of 1-4% be mixed with mixed liquor with sterile water, mix standby;
In described step S104, step S102 gained sodium alginate/pulvis mixed liquor is added in step S103 gained calcium chloride/pulvis mixed liquor with the speed of become dripping, to place the crosslinked time to be at least 30 minutes, gained preparation form is a milky white granules.
6, according to the described preparation method of claim 5, it is characterized in that:
In described step S102, it is 2% solution that the carrier sodium alginate is mixed with concentration, and pressure cooker sterilization back is standby, and it is 1% solution that step S101 gained pulvis sterile water is mixed with concentration, join in the solution of described sodium alginate according to 1: 1 volume ratio then, mechanical mixture is even;
In described step S103, crosslinking agent calcium chloride and step S101 gained pulvis are mixed with mixed liquor with 2% and 1% concentration with sterile water respectively, mix standby;
In described step S104, placing the crosslinked time is 60 minutes, gained preparation form is a milky white granules, by adjusting the size that step S102 gained sodium alginate/pulvis mixed liquor is added drop in step S103 gained calcium chloride/pulvis mixed liquor, the diameter that makes described preparation granules is 0.2～0.4mm, adjust the drop size and can obtain the different-grain diameter particle, satisfy the different application environment.
7, kill the application method of the slow release biological agent of chironomus larvas, it is characterized in that, in the applying environment in waters, water source, the usage amount of described slow release biological agent should reach 500～1000mg/m for making B.t.i pulvis active ingredient
2
According to the described application method of claim 7, it is characterized in that 8, the usage amount of described slow release biological agent reaches 600～700mg/m for making B.t.i pulvis active ingredient
2
9, kill the application method of the slow release biological agent of chironomus larvas, it is characterized in that, in the applying environment of water factory's structure, the usage amount of described slow release biological agent reaches 0.5～1.0mg/L for making B.t.i pulvis active ingredient.
According to the described application method of claim 9, it is characterized in that 10, the usage amount of described slow release biological agent reaches 0.6～0.7mg/L for making B.t.i pulvis active ingredient.
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| CN102038005A (en) * | 2010-12-15 | 2011-05-04 | 天津农学院 | Method for enhancing adsorption of Bacillus thuringiensis insecticidal proteins on mineral carrier |
| CN103749547A (en) * | 2013-12-25 | 2014-04-30 | 浙江农林大学 | Entomophthorales water floating type granules and application thereof |
| CN104773840A (en) * | 2015-04-15 | 2015-07-15 | 山西省农业科学院农业环境与资源研究所 | Slow-release compound flocculant for reducing acidity of mine water |
| CN104855428A (en) * | 2015-04-21 | 2015-08-26 | 福建农林大学 | Mosquito-killing bacillus thuringiensis floating agent and preparation method thereof |
| CN104855372A (en) * | 2015-05-21 | 2015-08-26 | 北京市环境保护科学研究院 | Method for preparing slow-release biological pesticide with polylactic acid as carrier and obtained product |
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| BR0003314B1 (en) * | 2000-05-24 | 2013-09-03 | Bacillus thuringiensis var israelensis-based bioinsecticide composition and its preparation process | |
| CN1240284C (en) * | 2002-04-23 | 2006-02-08 | 福建农林大学 | Biological pesticide synergist |
| MXPA02005713A (en) * | 2002-06-10 | 2004-08-12 | Consejo Del Sist Nac De Educac | FORMULATION OF CORN FLOUR OR GRITS PELLETS USING THE MIXTURE SPORE-CRYSTAL OF THE BACILLUS THURINGIENSIS ISRAELENSIS VARIETY ENCAPSULATED WITH SODIUM ALGINATE AND CALCIUM CHLORIDE FOR THE MOSQUITO's LARVAE CONTROL. |
| CN1332611C (en) * | 2003-01-10 | 2007-08-22 | 深圳市水务(集团)有限公司 | Preparation method of mosquito-killing preparation and its application |
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| CN102038005A (en) * | 2010-12-15 | 2011-05-04 | 天津农学院 | Method for enhancing adsorption of Bacillus thuringiensis insecticidal proteins on mineral carrier |
| CN102038005B (en) * | 2010-12-15 | 2013-03-20 | 天津农学院 | Method for enhancing adsorption of Bacillus thuringiensis insecticidal proteins on mineral carrier |
| CN103749547A (en) * | 2013-12-25 | 2014-04-30 | 浙江农林大学 | Entomophthorales water floating type granules and application thereof |
| CN103749547B (en) * | 2013-12-25 | 2017-09-08 | 浙江农林大学 | A kind of entomophthora water floats type granule and application thereof |
| CN104773840A (en) * | 2015-04-15 | 2015-07-15 | 山西省农业科学院农业环境与资源研究所 | Slow-release compound flocculant for reducing acidity of mine water |
| CN104855428A (en) * | 2015-04-21 | 2015-08-26 | 福建农林大学 | Mosquito-killing bacillus thuringiensis floating agent and preparation method thereof |
| CN104855372A (en) * | 2015-05-21 | 2015-08-26 | 北京市环境保护科学研究院 | Method for preparing slow-release biological pesticide with polylactic acid as carrier and obtained product |
| CN104920476A (en) * | 2015-06-11 | 2015-09-23 | 福建农林大学 | UV-resistant mosquito killing Bt slow release floating agent and preparation method thereof |
| CN110946150A (en) * | 2019-12-27 | 2020-04-03 | 武汉市拜乐卫生科技有限公司 | Compound mosquito killing oil suspension and preparation method thereof |
| CN110946150B (en) * | 2019-12-27 | 2021-08-27 | 武汉市拜乐卫生科技有限公司 | Compound mosquito killing oil suspension and preparation method thereof |
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