CN1332611C - Preparation method of mosquito-killing preparation and its application - Google Patents
Preparation method of mosquito-killing preparation and its application Download PDFInfo
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Abstract
The present invention discloses a preparation method of chironomus larvas killing preparations by Israel subspecies of bacillus thuringiensis, and an application method thereof. The preparation method comprising the following steps: sandy soil tube spores are inoculated on culture media on the oblique plane of an eggplant bottle for amplification culture, and cultured substances are manufactured into suspended seed liquor by sterilized water; the seed liquor is added to a fermentation tank to be cultured until spore capsules are broken and release spores and parasporal crystals; through post treatment of fermentation liquor, liquor, a powder or fixed dosage form preparation is obtained. The chironomus larvas killing preparation has special toxic effect on chironomid with colored wings, and the three-instar larval killing ratio reaches 98% in 24 hours within the working concentration. When used for treating chironomus pollution bred in urban water resources and water supply facilities, the microbiological preparation meets requirements on environmental protection and low residue; with various dosage forms, the microbiological preparation is suitable for water areas with different features, and has the advantages of high efficiency, concentration and slow release.
Description
Technical field
The present invention relates to the water prevention and cure of pollution technology, be specifically related to adopt the technology of microbial control water body insect contamination, more particularly, relate to a kind of method that adopts bacillus thuringiensis subsp israelensis to prepare the method for the kill mosquito preparation of preventing and treating chironomus larvas and use chironomus larvas pollution in this kill mosquito preparation elimination water body.
Background technology
Chironomidae (Chironomidae) is with tipulidae, Dulicidae, Heleidae, Simulidae etc., belong to diptera (Duotera) Nematocera (Nematocera), belong to complete metamorphosis, the whole growth growth course comprises ovum, larva, pupa and adult four-stage.Its larva has another name called red worm or Tubifex, and living worm body is cylindrical, merogenesis, cerise.Midge does not bite people and animals' behavior, is distributed widely in the mud of water bodys such as pond, irrigation canals and ditches, lake, reservoir.The Chironomidae larva is a kind of crucial zoobenthos, with the various organic matters of water-bed and pool wall and algae etc. is food, the activity of chironomus larvas in water body deposit influences sedimental character, for the biology and the very important influence of physical property generation of water environment.In addition, midge also is various carriers of pathogens, the multiple germ of portability typhoid bacillus and paratyphoid, and a few species can be brought out irritated allergic diseases such as human asthma.At present, kind surplus known in the world Chironomidae kind has reached 3000 is distributed widely in Russia, Korea, Japan, Lebanon, Europe, North America and China.
In reservoir water source, city, often there are a large amount of midges to multiply, its worm's ovum and larva enter the water supply treatment process facility in city with former water, owing to the suitable living environment of organic nutrient substance such as algae abundant in the former water and sedimentation basin causes midge and larva thereof to multiply breeding at water facilities.Its larva (being commonly called as red worm) often penetrates sand filter blanket and enters pipe network, not only influences the water quality organoleptic indicator, and pathophorous potential danger is arranged.
Red worm control to urban water resource and water system is the big technical barrier on boundary that supplies water both at home and abroad always, main cause is that red worm breeding is rapid, tolerance to environment is stronger, be difficult to contact with chlorine and be inactivated, general physical prevents and treats method and chemical agent also can't use in water source and water system, thereby lacks effective governing measure for a long time.
In insect control, select also rationally to use nuisanceless medicament very important to urban water resource.Than chemical insecticide, microbial control can be efficiently, low toxicity, the chironomus larvas that prevents and treats in water source and the water treatment procedure safely pollute, and has special benefits and application prospects.Microbial strains bacillus thuringiensis subsp israelensis (the Bacillus thuringiensis srbsp.Iaraelenses that is used for mosquito control, be called for short Bti), its parasporal crystal has single-minded toxic action to dipteral insect, dissolving and discharge toxic fragment under the distinctive strong basicity midgut of insect environment, act on insect midgut epithelial cell tissue, cause that microvillus zone swelling, endoplasmic reticulum cavity form, follow the ribosome forfeiture and, cause the rapid death of mosquito mitochondrial various damages.Crystalline protein has special toxic effect to the diptera mosquito, other are comprised that fish, amphibian animal, birds and mammal are nontoxic, the kill mosquito practice of Bti is carried out for many years in the U.S., France, the former Soviet Union, China, Indonesia, Egypt and West Africa, and a large amount of toxicology tests and the application in countries in the world confirm their good safeties to environment and other biological.
Bti applies to bite killing of blood mosquito always, though chironomus larvas is had certain toxic effect (external existing studies confirm that), never is applied to the Chironomidae insect control.Through to the retrieval of relevant patent both at home and abroad, also find no and be applied to the especially content of chironomus larvas in the water system of water environment.
Summary of the invention
The technical problem that the present invention will solve is, the preparation method who provides a kind of chironomus larvas to kill preparation, and prepared kill mosquito preparation can effectively be killed red worm, and safety non-toxic, does not produce water body chemical and pollutes.Preparation with this method preparation can be applicable to that different water bodys carries out the kill mosquito control;
Another technical problem that the present invention will solve is, a kind of application method of above-mentioned kill mosquito preparation being killed chironomus larvas at different water bodys is provided.
The preparation method who provides a kind of chironomus larvas to kill preparation, described preparation is a kind of liquid dosage form, the gemma of bacillus thuringiensis subsp israelensis and the content of parasporal crystal are not less than 10 in every milliliter of the described liquid preparation
9Individual, its preparation method comprises the steps:
1) sand tube spore is inoculated into eggplant bottle slant culture (first kind of medium), 30 ℃ of following enlarged culture 72 hours;
2) will cultivate lawn and add 30 milliliters of sterile waters, make suspension, handle 15 minutes down for 75 ℃;
3) fermentation: suspension is directly added jar fermenter, be cultured to sporangium and break and discharge gemma and parasporal crystal (second kind of medium), fermentation time is about 40 hours; During fermentation, the cylinder temperature remains on 30 ± 1 ℃; Cylinder is pressed and is remained on 0.3-0.5kg/cm
2Throughput per minute 1: 1, speed of agitator 220-240rpm;
4) the zymotic fluid acidification (is promptly used H
2SO
4Transfer PH to 4.3), and adopt plate compression to obtain the kill mosquito preparation of liquid dosage form.
The preparation method of another kind at the kill mosquito preparation of Chironomidae larva is provided, and described preparation is a kind of powder formulation, and its preparation method comprises the steps:
1) sand tube spore is inoculated into eggplant bottle slant culture (first kind of medium), 30 ℃ of following enlarged culture 72 hours;
2) will cultivate lawn and add 30 milliliters of sterile waters, make suspension, handle 15 minutes down for 75 ℃;
3) fermentation: suspension is directly added jar fermenter, be cultured to sporangium and break and discharge gemma and parasporal crystal (second kind of medium), fermentation time is about 40 hours; During fermentation, the cylinder temperature remains on 30 ± 1 ℃; Cylinder is pressed and is remained on 0.3-0.5kg/cm
2Throughput per minute 1: 1, speed of agitator 220-240rpm;
4) zymotic fluid is adsorbed with filler, adopt plate compression to form filter cake, with the filter cake making beating, add auxiliary agent again, atomized drying obtains powder-type kill mosquito preparation.
The preparation method of another kind of kill mosquito preparation at the Chironomidae larva is provided, and described preparation is a kind of zymocyte liquid to be sprayed on immobilization formulation on the carrier, and its preparation method comprises the steps:
1) sand tube spore is inoculated into eggplant bottle slant culture (first kind of medium), 30 ℃ of following enlarged culture 72 hours;
2) will cultivate lawn and add 30 milliliters of sterile waters, make suspension, handle 15 minutes down for 75 ℃;
3) fermentation: suspension is directly added jar fermenter, be cultured to sporangium and break and discharge gemma and parasporal crystal (second kind of medium), fermentation time is about 40 hours; During fermentation, the cylinder temperature remains on 30 ± 1 ℃; Cylinder is pressed and is remained on 0.3-0.5kg/cm
2Throughput per minute 1: 1, speed of agitator 220-240rpm;
4) the bacterium liquid after will fermenting evenly is sprayed on the nonwoven carrier, and every gram nonwoven spraying concentration is about 0.5 milliliter of 10% zymocyte liquid;
5) spraying assisted immobilization material sodium alginate soln, the spraying weight of every gram nonwoven is about 0.15 gram;
6) evenly spraying concentration again on the assisted immobilization material is about 0.5 milliliter of 10% zymocyte liquid;
7) the main immobilization material poly-vinyl alcohol solution of spraying; The spraying weight of every gram nonwoven is 0.34 gram;
8) envelope volume after the natural seasoning forms immobilization kill mosquito preparation.
First kind of medium among the above-mentioned preparation method is made of the material of following weight ratio: beef extract 0.3%, and peptone 1%, sodium chloride 0.5%, agar 1.5-2.0%, pH value is 7.0-7.2, at 1.05Kg/cm
2Pressure and 121.3 ℃ condition under sterilization made in 20 minutes.
Second kind of medium among the above-mentioned preparation method is made of the material of following weight ratio: corn steep liquor 2%, and soybean meal 2.5%, corn starch 2%, other mineral salt, pH value is 7.2, at 1.05Kg/cm
2Pressure and 121.3 ℃ condition under sterilization made in 20 minutes.
In above-mentioned preparation method, contain sodium alginate 0.15g in every gram nonwoven, polyvinyl alcohol 0.34g, liquor product 1.2 grams, 5 milliliters in water.
More than the bacillus thuringiensis subsp israelensis gemma and the parasporal crystal that comprise in three kinds of products have following feature:
Gemma is oval, and diameter 1.0 * 1.5 μ m have stronger resistance to poor environment, can sprout to be the bacillus thuringiensis,Bt trophosome under control environment.Tool warm tolerance (can survive after 20 minutes) 75 ℃ of processing.
Parasporal crystal is the compound protein polymer of almost spherical, differs in size, and diameter range 0.5～1.2 μ m, and contain carbohydrate contents such as glucose and mannose.
Parasporal crystal protein by protease hydrolytic, becomes the toxic polypeptide of 24～25kDa under insect midgut strong basicity environment, produce the strong toxicity to the effect insect.Gemma strengthens the virulence of parasporal crystal to insect.
Above-described sand tube spore refers to a kind of culture presevation mode, is about to above-mentioned bacterial classification and is placed in the sterilization sandy soil pipe that vacuumizes and preserves.
The liquid dosage form that makes or powder formulation kill mosquito preparation are applied in standing waters such as pond kill chironomus larvas, the working concentration of liquid mosquitocide is 100ppm, and the working concentration of pulvis is 20ppm, by estimating water yield sprinkling every day, continue 15 days, life cycle is 30 days.
The immobilization formulation kill mosquito preparation that makes is applied in the waters of flowing carries out water treatment, lays immobilization formulation kill mosquito preparation more than one meter along pool wall, replacing in per 10 days once, life cycle is 30 days.
The kill mosquito preparation that adopts said method to make supplies water and multiplies killing of red worm in the environment and have following advantage: the gemma and the parasporal crystal that obtain through the fermentation to bacillus thuringiensis subsp israelensis, Chironomidae insect Chironomus kiiensis had special toxic action, can be safely, economical, prevent and treat the red worm that multiplies in the water supply environment effectively, solve the technical barrier that the puzzlement municipal water is handled, prepared microbial inoculum is suitable for using in water source and water treatment technology, has the red worm killing effect of stability and high efficiency.After tested, in the 80ppm concentration 24 hours to 3 ages red worm larva killing rate reach 98%.Adopt microorganism that the midge that multiplies in urban water resource and the water facilities is polluted and administer, meet the requirement of environmental protection, low-residual, have efficient, safe characteristics.The multiple formulation that is provided is applicable to different waters.
Water system is typical running system, and the bloodthirsty mosquito of existing other type is killed biologic product can only be applied to the water body that pond, ditch etc. are static or the water flows gently.The thalline fixed dosage form has efficiently, concentrates, the advantage of slowly-releasing, the long-term toxic effect of maintenance.
Drinking water production has very high water quality safety requirement, for avoiding in the water treatment environment, causing bacterium and insecticidal proteins to pollute, influence the organic indicator and the toxicologic index of water quality, pass through immobilization technology, and cooperate water treatment technologies such as coagulation, sterilization, filtration, it is residual with the water outlet of killing crystalline protein to eliminate microorganism after the using microbe preparation carries out red worm control to greatest extent, guarantees water quality safety.
Behind the using microbe kill mosquito preparation, the following index of handling water outlet can reach national drinking water safety sanitary standard: total number of bacteria, COD in the output water
Mn, mutagenesis index (Salmonella reversion test).
Embodiment
The bacillus thuringiensis subsp israelensis (Bti) that the present invention adopts is the subspecies that in the bacillus thuringiensis,Bt mosquito larvae had special toxicity, serotype is H14, its toxic effect characteristics are nontoxic to lepidopterous insects, and dipteral insect (mainly being mosquito) is had special toxic effect.Bacillus thuringiensis subsp israelensis (Bti) has following biological property:
It is shaft-like that trophosome is, about 4.2～5.8 * 1.2～1.3um, and the Gram reacting positive can be grown on multiple medium.The long oval of its sporangium, an end forms avette gemma, and the other end produces parasporal crystal.Sporangium breaks behind certain hour, discharges free parasporal crystal and spore.
Bacterium colony rough surface and protuberance on the plain broth protein culture medium, dark white produces pale purple pigment, and the edge is thin slightly, and is irregular.Lawn edge, inclined-plane is the rhizoid shape.
Form film in broth bouillon, easily the concassation slabbing sinks.
Embodiment one: the kill mosquito preparation of preparation liquid dosage form,
1) sand tube spore is inoculated into eggplant bottle slant culture, (first kind of medium) was 30 ℃ of following enlarged culture 72 hours;
2) will cultivate lawn and add 30 milliliters of sterile waters, make suspension, handle 15 minutes down for 75 ℃;
3) fermentation: suspension is directly added jar fermenter, be cultured to sporangium and break and discharge gemma and parasporal crystal (second kind of medium), fermentation time is about 40 hours; During fermentation, the cylinder temperature remains on 30 ± 1 ℃; Cylinder is pressed and is remained on 0.3-0.5kg/cm
2Throughput per minute 1: 1, speed of agitator 220-240rpm.
4) the zymotic fluid acidification (is promptly used H
2SO
4Transfer PH to 4.3), and adopt plate compression to obtain the kill mosquito preparation of liquid dosage form.
First kind of medium is made of the material of following weight ratio: beef extract 0.3%, and peptone 1%, sodium chloride 0.5%, agar 1.5-2.0%, pH value is 7.0-7.2, at 1.05Kg/cm
2Pressure and 121.3 ℃ condition under sterilization made in 20 minutes.
Second kind of medium is made of the material of following weight ratio: corn steep liquor 2%, and soybean meal 2.5%, corn starch 2%, other mineral salt, pH value is 7.2, at 1.05Kg/cm
2Pressure and 121.3 ℃ condition under make after the sterilization.
The capacity of jar fermenter is 15 tons; Feed intake 60% in the cylinder;
When liquid dosage form being applied to chironomus larvas in the standing water such as pond and administering, working concentration is 100ppm, by estimating that the water yield sprays every day, continues 15 days, and life cycle is 30 days.
Embodiment two: prepare the method for powder-type kill mosquito preparation, adopt following steps to prepare pulvis after the fermentation step in embodiment one is finished:
Zymotic fluid is adsorbed with filler calcium carbonate, adopt plate compression to form filter cake, with the filter cake making beating, add auxiliary agent calcium carbonate again, atomized drying obtains powder-type kill mosquito preparation.
When the powder formulation kill mosquito preparation that makes was applied to the chironomus larvas improvement of standing waters such as pond, working concentration was 20ppm, by estimating water yield sprinkling every day, continued 15 days, and life cycle is 30 days.
Embodiment three: adopt following steps can make fixed kill mosquito preparation after the fermentation step in embodiment one is finished:
Bacterium liquid after the fermentation evenly is sprayed on the nonwoven carrier, and every gram nonwoven spraying concentration is 0.5 milliliter of 10% liquor; At bacterium liquid surface spraying assisted immobilization material sodium alginate soln, spraying 0.15 gram of every gram nonwoven; On the assisted immobilization material, evenly spray concentration again and be 10% zymocyte liquid 0.5mL; At bacterium liquid surface spraying main immobilization material polyvinyl alcohol (PVA) solution, spraying weight 0.34 gram of every gram nonwoven; The envelope volume is secured to the kill mosquito preparation on the carrier after the natural seasoning.
In every gram nonwoven, contain sodium alginate 0.15 gram, polyvinyl alcohol 0.34 gram, liquor product 1.2 grams, 5 milliliters in water.
The immobilization formulation kill mosquito preparation that makes is applied in the waters chironomus larvas of flowing and administers, and can lay immobilization formulation kill mosquito preparation more than one meter along pool wall, changes once in per 10 days, and life cycle is 30 days.
Immobilization material does not influence Bti to mosquito larvae virulence effect, and the immobilization material of use does not influence water quality safety.Its production method and technology are convenient simple and efficient, suitable for mass production; In effective life, can effectively be fixed in the pool wall of water factory's sedimentation basin and swash plate etc. and locate, suit to use at water factory's sedimentation basin; Be easy to preserve; 24 hours lethality rates of Chironomus kiiensis larva are reached 98%; Have slowly-releasing insecticidal crystal ability, its effective life, be limited to 15 days.
When being used for the water treatment environment, must formulate best dispensing cycle and frequency according to water treatment factors such as chlorine ion concentration, illumination, coagulant, determine injected volume, release position etc., guarantee the killing rate of the red worm of sedimentation basin, and adopt associated safety control application technology, comprise the removing or the means such as relevant water treatment technology parameter such as recovery, adjustment coagulation intensity, filtration interception capacity and chlorination of regularly carrying out biologic product.
Claims (7)
1, a kind of bacillus thuringiensis subsp israelensis that adopts prepares the method that chironomus larvas is killed preparation, and described preparation is a liquid dosage form, and the gemma of bacillus thuringiensis subsp israelensis and the content of parasporal crystal are not less than 10 in every milliliter of the described liquid preparation
9Individual, its preparation method comprises the steps:
1) sand tube spore is inoculated into eggplant bottle slant culture, in bottle, puts into first kind of medium, 30 ℃ of following enlarged culture 72 hours;
2) will cultivate lawn and add sterile water 30mL, make suspension, handle 15 minutes down at 75 ℃;
3) fermentation: suspension is directly added jar fermenter, be cultured to sporangium and break and discharge gemma and parasporal crystal, put into second kind of medium in cylinder, fermentation time is about 40 hours; During fermentation, the cylinder temperature remains on 30 ± 1 ℃; Cylinder is pressed and is remained on 0.3-0.5kg/cm
2Throughput per minute 1: 1, speed of agitator 220-240rpm;
4) with the zymotic fluid acidification, use H
2SO
4Transfer PH to 4.3, and adopt plate compression to obtain the kill mosquito preparation of liquid dosage form;
Wherein, described first kind of medium is made of the material of following weight ratio: beef extract 0.3%, and peptone 1%, sodium chloride 0.5%, agar 1.5-2.0%, pH value is 7.0-7.2, at 1.05Kg/cm
2Pressure and 121.3 ℃ condition under sterilization made in 20 minutes;
Described second kind of medium is made of the material of following weight ratio: corn steep liquor 2%, and soybean meal 2.5%, corn starch 2%, other mineral salt, pH value is 7.2, at 1.05Kg/cm
2Pressure and 121.3 ℃ condition under make after the sterilization.
2, a kind of bacillus thuringiensis subsp israelensis that adopts prepares the preparation method that chironomus larvas is killed preparation, and described formulation is the powder formulation, and its preparation method comprises the steps:
1) sand tube spore is inoculated into first kind of medium, under 30 ℃ eggplant bottle inclined-plane enlarged culture 72 hours;
2) will cultivate lawn and add sterile water 30mL, make suspension, handle 15 minutes down for 75 ℃;
3) fermentation: suspension is directly added jar fermenter, be cultured to sporangium and break and discharge gemma and parasporal crystal in second kind of medium, fermentation time is about 40 hours; During fermentation, the cylinder temperature remains on 30 ± 1 ℃; Cylinder is pressed and is remained on 0.3-0.5kg/cm
2Throughput per minute 1: 1, speed of agitator 220-240rpm;
4) zymotic fluid is adsorbed with calcium carbonate-filled dose, adopt plate compression to form filter cake, with the filter cake making beating, add calcium carbonate builder again, atomized drying obtains powder-type kill mosquito preparation;
Wherein, described first kind of medium is made of the material of following weight ratio: beef extract 0.3%, and peptone 1%, sodium chloride 0.5%, agar 1.5-2.0%, pH value is 7.0-7.2, at 1.05Kg/cm
2Pressure and 121.3 ℃ condition under sterilization made in 20 minutes;
Described second kind of medium is made of the material of following weight ratio: corn steep liquor 2%, and soybean meal 2.5%, corn starch 2%, other mineral salt, pH value is 7.2, at 1.05Kg/cm
2Pressure and 121.3 ℃ condition under make after the sterilization.
3, a kind of bacillus thuringiensis subsp israelensis that adopts prepares the preparation method that the Chironomidae larva is killed preparation, and described preparation is the immobilization formulation that a kind of zymocyte liquid is fixed through carrier, and its preparation method comprises the steps:
1) sand tube spore is inoculated into first kind of medium, under 30 ℃ eggplant bottle inclined-plane enlarged culture 72 hours;
2) will cultivate lawn and add sterile water 30mL, make suspension, handle 15 minutes down for 75 ℃;
3) fermentation: suspension is directly added jar fermenter, be cultured to sporangium and break and discharge gemma and parasporal crystal in second kind of medium, fermentation time is about 40 hours; During fermentation, the cylinder temperature remains on 30 ± 1 ℃; Cylinder is pressed and is remained on 0.3-0.5kg/cm
2Throughput per minute 1: 1, speed of agitator 220-240rpm;
4) the bacterium liquid after will fermenting evenly is sprayed on the nonwoven carrier, and every gram nonwoven spraying concentration is 0.5 milliliter of 10% zymocyte liquid;
5) spraying assisted immobilization material sodium alginate soln, the spraying weight of every gram nonwoven is about 0.15 gram;
6) evenly spraying concentration again on the assisted immobilization material is about 0.5 milliliter of 10% zymocyte liquid;
7) the main immobilization material poly-vinyl alcohol solution of spraying; The spraying weight of every gram nonwoven is 0.34 gram;
8) envelope volume after the natural seasoning forms immobilized kill mosquito preparation;
Wherein, described first kind of medium is made of the material of following weight ratio: beef extract 0.3%, and peptone 1%, sodium chloride 0.5%, agar 1.5-2.0%, pH value is 7.0-7.2, at 1.05Kg/cm
2Pressure and 121.3 ℃ condition under sterilization made in 20 minutes;
Described second kind of medium is made of the material of following weight ratio: corn steep liquor 2%, and soybean meal 2.5%, corn starch 2%, other mineral salt, pH value is 7.2, at 1.05Kg/cm
2Pressure and 121.3 ℃ condition under make after the sterilization.
4, according to the described preparation method of claim 3, it is characterized in that, contain sodium alginate 0.15 gram in every gram nonwoven, polyvinyl alcohol 0.34 gram, liquor product 1.2 grams, water 5mL.
5, the liquid dosage form that claim 1 is made is applied in and carries out the method that mosquito larvae is killed in the hydrostatic territory, it is characterized in that, the working concentration of liquor is 100ppm, by estimating water yield sprinkling every day, continues 15 days, and life cycle is 30 days.
6, the powder formulation kill mosquito preparation that claim 2 is made is applied in and carries out the method that mosquito larvae is killed in the hydrostatic territory, it is characterized in that the working concentration of pulvis is 20ppm, by estimating water yield sprinkling every day, continues 15 days, and life cycle is 30 days.
7, the immobilization formulation kill mosquito preparation that claim 3 or 4 is made is applied in the waters of flowing and carries out the method that mosquito larvae is killed, and it is characterized in that, lays immobilization formulation kill mosquito preparation more than one meter along pool wall, changes once in per 10 days, and life cycle is 30 days.
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| CN1332611C true CN1332611C (en) | 2007-08-22 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN100366156C (en) * | 2005-06-03 | 2008-02-06 | 深圳市水务(集团)有限公司 | Slow release biological agent for killing larvae of midge, preparation method and application method |
| BE1018872A5 (en) * | 2009-08-28 | 2011-10-04 | Cruysberghs Rudiger | PROTECTION AGAINST MUGS. |
| CN107926984A (en) * | 2017-11-29 | 2018-04-20 | 江苏源洁节能环保有限公司 | A kind of biological insecticides for water environment |
| CN107889836A (en) * | 2017-11-29 | 2018-04-10 | 北京华夏源洁水务科技有限公司 | A kind of biological insecticides for water environment |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4133889A1 (en) * | 1991-10-12 | 1993-04-15 | Norbert Dr Becker | Effervescent tablets for mosquito larvae control - contg. Bacillus Thuringiensis and Bacillus Sphaericus endotoxin, avoid toxic risks and environmental damage |
| DE10062780A1 (en) * | 2000-12-15 | 2002-07-11 | Olaf Becker | Bacillus thuringiensis israelensis endotoxin-cellulose granulate, used for killing Chironomidae midge larvae in deep or running water, has sufficient high density to sink rapidly in water |
| WO2002065835A2 (en) * | 2001-02-16 | 2002-08-29 | Valent Biosciences Corp. | Mixture of bacillus thuringiensis subspecies israelensis and bac illus sphaericus for management of resistance to mosquito larvicides |
| CN1368549A (en) * | 2002-03-06 | 2002-09-11 | 中国科学院武汉病毒研究所 | Tharingiensis bacillus strain with broad-spectrum insecticiding activity and its preparing process |
-
2003
- 2003-01-10 CN CNB031135552A patent/CN1332611C/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4133889A1 (en) * | 1991-10-12 | 1993-04-15 | Norbert Dr Becker | Effervescent tablets for mosquito larvae control - contg. Bacillus Thuringiensis and Bacillus Sphaericus endotoxin, avoid toxic risks and environmental damage |
| DE10062780A1 (en) * | 2000-12-15 | 2002-07-11 | Olaf Becker | Bacillus thuringiensis israelensis endotoxin-cellulose granulate, used for killing Chironomidae midge larvae in deep or running water, has sufficient high density to sink rapidly in water |
| WO2002065835A2 (en) * | 2001-02-16 | 2002-08-29 | Valent Biosciences Corp. | Mixture of bacillus thuringiensis subspecies israelensis and bac illus sphaericus for management of resistance to mosquito larvicides |
| CN1368549A (en) * | 2002-03-06 | 2002-09-11 | 中国科学院武汉病毒研究所 | Tharingiensis bacillus strain with broad-spectrum insecticiding activity and its preparing process |
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