CN1861787A - Cell dispersion treating agent - Google Patents
Cell dispersion treating agent Download PDFInfo
- Publication number
- CN1861787A CN1861787A CNA2005100435396A CN200510043539A CN1861787A CN 1861787 A CN1861787 A CN 1861787A CN A2005100435396 A CNA2005100435396 A CN A2005100435396A CN 200510043539 A CN200510043539 A CN 200510043539A CN 1861787 A CN1861787 A CN 1861787A
- Authority
- CN
- China
- Prior art keywords
- cell
- treating agent
- solution
- dispersion treating
- cell dispersion
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a finishing agent for the cell dispersion which makes the cells of different size and tissue mass with many cells carry the same charges by the interface chemical technology. So it has the anti-aggregation effect in the limit liquid. Concretely speaking, the cell suspension for smearing is added into the proper finishing agent such as the butyl naphthalene sulfonic acid sodium 0.1-10% to make the cell thin and uniform in the slide. Also, it is benefit for the storage of the cell.
Description
Technical field
The present invention relates to a kind of cell and disperse and observation technology, particularly relate to the detection of uterus carcinoma cell.
Background technology
In the detection of cell, the observation cellular form is a microbiological important means, usually we to observe a certain tissue be that sampling cell is placed on the slide, in the morphological specificity of microscopically observation cell, thus definite.But at one, its form just is difficult to observation comes out, and can't detect as if cell accumulation, and for this reason, people invent a kind of equipment and by the negative pressure centrifugation cell spread out so that observe.But because equipment is seen costliness, therefore, the testing cost height, and be difficult for popularizing.
Summary of the invention
The objective of the invention is to develop a kind of cell-dispersing agent cell is spread out, be convenient to the observation of cell.
Another object of the present invention is beneficial to the preservation of cell simultaneously, and is the preservation liquid of environmental protection.
Technical scheme of the present invention is to utilize the interfacial chemistry technology, makes cell that differs in size and the identical electric charge of tissue block band that contains a plurality of cells, produces anti-accumulative effect in limited solution.As use dispersion agent that cell is spread out in solution.
Described dispersion agent is a kind of anion surfactant.It can be sulfonate such as dodecylbenzene alkyl sulfonate, butyl naphthalene sulfonate etc.Its consumption is the 0.1-10% of solution amount.
Described cell dispersion treating agent can be used as a kind of environment-friendly type cell-preservation liquid simultaneously, and this cell-preservation liquid uses nontoxic essence to form with the alcohol dilution of lower concentration, as natural vanillin and Geraniol 0.1---5%.Having overcome traditional stationary liquid usually will be with the disadvantage of toxic methyl alcohol or aldehyde material.
Before this invention, traditional smear is just by realizing all even thickness that cell is relative by the suitable angle of smearing direction and another boosting slide with dynamics.And the present invention only need add the small amounts of cells dispersion agent in the cell tissue sampling, observes getting final product on slide.
Embodiment
Embodiment 1: be sampled as the uterus carcinoma tissue, be made at cell suspension for smear, in solution, add dispersion agent sodium butylnaphthalenesulfonate 0.1% (weight ratio), vanillin food grade,1000.000000ine mesh 0.1%, make cell in slide, keep thin and uniform distribution, and this tissue solution can be stored the long period, examine under a microscope above-mentioned solution, cell has obtained good dispersion.
Embodiment 2: be sampled as the uterus carcinoma tissue, be made at cell suspension for smear, in solution, add dispersion agent sodium butylnaphthalenesulfonate 10% (weight ratio), vanillin food grade,1000.000000ine mesh 5%, make cell in slide, keep thin and uniform distribution, and this tissue solution can be stored the long period, examine under a microscope above-mentioned solution, cell has obtained good dispersion.
Claims (5)
1, a kind of cell decentralized approach utilizes the interfacial chemistry technology, makes cell that differs in size and the identical electric charge of tissue block band that contains a plurality of cells, produces anti-accumulative effect in limited solution, and cell is spread out in solution.
2, a kind of cell dispersion treating agent is characterized in that in common cell suspending liquid, adds anion surfactant, and consumption is the 0.1-10% (weight ratio) of solution amount.
3, cell dispersion treating agent according to claim 2 is characterized in that anion surfactant is a sodium butylnaphthalenesulfonate.
4, cell dispersion treating agent according to claim 2 is characterized in that the observation of uterus carcinoma cell.
5, cell dispersion treating agent according to claim 2 is characterized in that also containing nontoxic essence spirituous solution, and is 0.1-5% (weight ratio).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2005100435396A CN1861787A (en) | 2005-05-12 | 2005-05-12 | Cell dispersion treating agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2005100435396A CN1861787A (en) | 2005-05-12 | 2005-05-12 | Cell dispersion treating agent |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1861787A true CN1861787A (en) | 2006-11-15 |
Family
ID=37389319
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2005100435396A Pending CN1861787A (en) | 2005-05-12 | 2005-05-12 | Cell dispersion treating agent |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1861787A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108308172A (en) * | 2017-12-26 | 2018-07-24 | 博朗（厦门）生物科技有限公司 | A kind of Sample storage liquid for parasitic ovum detection |
-
2005
- 2005-05-12 CN CNA2005100435396A patent/CN1861787A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108308172A (en) * | 2017-12-26 | 2018-07-24 | 博朗（厦门）生物科技有限公司 | A kind of Sample storage liquid for parasitic ovum detection |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Fukui et al. | Mating pheromone‐like diffusible factor released by Schizosaccharomyces pombe | |
| CN105645829B (en) | Method for improving stability of foaming agent | |
| Burattini et al. | Analysis of cell death by electron microscopy | |
| Capson-Tojo et al. | Mesophilic and thermophilic anaerobic digestion of lipid-extracted microalgae N. gaditana for methane production | |
| CN103254683B (en) | Water-based color paste binder, and preparation method and application thereof | |
| CN1749348A (en) | Glass fog-proof agent and its preparing method | |
| CN108927073A (en) | A kind of anacardol amino acid surfactant and preparation method thereof | |
| GB2500721A (en) | Cell culture medium additive comprising a xanthan polysaccharide and a mannan polysaccharide | |
| Rasool et al. | Inhibitory effects of silver nanoparticles on removal of organic pollutants and sulfate in an anaerobic biological wastewater treatment process | |
| CN1861787A (en) | Cell dispersion treating agent | |
| Guerra et al. | Biochemical and microbiological tools for the evaluation of environmental quality of a coastal lagoon system in Southern Brazil | |
| CN109638286B (en) | Carboxymethyl cellulose-based binder and application thereof in lithium battery | |
| CN103694958B (en) | Glass antifogging agent and preparation method thereof | |
| WO2024001705A1 (en) | Evaluation method for biological composite oil displacement system and construction method for biological composite oil displacement system | |
| CN103360889B (en) | Stone matter colored drawing strengthening agent | |
| CN104032289B (en) | Method for corrosion resistance and tarnishing resistance treatment of gold and its alloy surface | |
| CN113493724B (en) | Efficient decontamination laundry detergent and preparation method thereof | |
| Rychtecký et al. | Light availability may control extracellular phosphatase production in turbid environments | |
| CN102911822A (en) | Fabric detergent and preparation method thereof | |
| CN106867456A (en) | A kind of high-efficient corrosion inhibition type villaumite type Snow Agent | |
| CN100541197C (en) | A kind of indoor link plate evaluation method of nonpolluting coating antifouling property | |
| CN103397330B (en) | A kind of environment-friendly type aqueous rust-preventive agent and preparation method thereof | |
| CN102835387A (en) | Method for preparing sealed glass slide for microscope | |
| CN104726863A (en) | Corrosive for displaying ultralow carbon-steel casting blank hook-shaped slab shell as well as preparation method and preparation method for corrosive | |
| CN110229307A (en) | A kind of poly- polyisocyanates shape memory aeroge of HEC/CNC/ and its preparation method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |