CN108308172A - A kind of Sample storage liquid for parasitic ovum detection - Google Patents
A kind of Sample storage liquid for parasitic ovum detection Download PDFInfo
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- CN108308172A CN108308172A CN201711433166.2A CN201711433166A CN108308172A CN 108308172 A CN108308172 A CN 108308172A CN 201711433166 A CN201711433166 A CN 201711433166A CN 108308172 A CN108308172 A CN 108308172A
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- parasitic ovum
- detection
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- storage liquid
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
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Abstract
The present invention relates to the Sample storage liquid for parasitic ovum detection described in a kind of Sample storage liquid for parasitic ovum detection and application method to include：Malachite green, pH buffer, dodecyl sodium sulfate, paraformaldehyde, methanol, ethyl alcohol, glycerine, L alanine etc. are formulated.The beneficial effects are mainly as follows：(1) present invention, which preserves liquid, can be applicable in the preservation and processing of humans and animals excrement, cost is relatively low, it long-term preservation and can be readily transported in the case that keeping the complete sample that preferably solves of parasitic ovum, improve environment when detection, the form for the parasitic ovum being stored in liquid basal cell treatment fluid of the present invention is set to have obtained good fixation and holding, the swelling rupture and atrophy of parasitic ovum are avoided, convenient for diagnosis.(2) present invention is used for the Sample storage liquid of parasitic ovum detection, and containing in component keeps visual field background soft, reduces the component of visual field fatigue, convenient for observing.
Description
(1) technical field
The present invention relates to a kind of Sample storage liquid for parasitic ovum detection, and utilize described for parasitic ovum inspection
The method of parasitic ovum in the Sample storage liquid detection excrement of survey.
(2) background technology
Parasitic ovum invades host, can generate a variety of harm and influence the health of host.Parasitic ovum can be with host
Excrement excrete, therefore can be detected whether by excrement infection parasite and infection degree.Detect parasitic ovum
Common method, as directly checked the polypide in excrement, physiological saline smear method adds rattan method etc., but these methods are by certain
The limitation of condition, if sample is unable to long-term preservation, stink is heavier when detection, take etc..
(3) invention content
For the deficiency of existing excrement parasite detection technique, the object of the present invention is to provide one kind quickly to detect,
Long period preserves fecal sample, and improves the environment of detection excrement, reduces being detected for parasitic ovum for stink when detection
Sample storage liquid, and utilize the side of parasitic ovum in the Sample storage liquid detection excrement for parasitic ovum detection
Method.
The technical solution adopted by the present invention is：
A kind of Sample storage liquid for parasitic ovum detection includes mainly following components：
50~100g/L of paraformaldehyde, 10~30g/L of methanol, 10~50g/L of ethyl alcohol, 0.3~0.8g/L of l-Alanine, L-
0.3~0.8g/L of isoleucine, 0.005~0.015mol/L of phosphate buffer, 0.1~0.3g/L of lauryl sodium sulfate,
5~10g/L of vanillic aldehyde, 0.05~0.09g/L of malachite green, 30~80g/L of glycerine adjust pH 6.2~6.8, and surplus is water.
Preferably, it forms as follows：
60~80g/L of paraformaldehyde, 20~25g/L of methanol, 20~30g/L of ethyl alcohol, 0.5~0.6g/L of l-Alanine, L-
0.4~0.6g/L of isoleucine, 0.008~0.01mol/L of phosphate buffer, 0.15~0.2g/L of lauryl sodium sulfate,
6~7g/L of vanillic aldehyde, 0.06~0.07g/L of malachite green, 50~60g/L of glycerine adjust pH 6.5, and surplus is water.
Its preparation method is as follows：By the paraformaldehyde of formula ratio, the methanol, the ethyl alcohol, the L- third
Propylhomoserin, the l-Isoleucine, the phosphate buffer, the lauryl sodium sulfate, the vanillic aldehyde, the malachite
The green and described glycerine is dissolved in the water, adjusts the pH value to 6.2~6.8, then plus the water is settled to certain body
Product.
Malachite green is added in the Sample storage liquid for parasitic ovum detection, visual field when the purpose is to be conducive to observation
It is soft, mitigate the fatigue of eyes.It is added to after paraformaldehyde in the Sample storage liquid for parasitic ovum detection, excrement
Sample can preserve 12 days or more, easy to carry and transport.The Sample storage formula of liquid side for parasitic ovum detection
Just, easy to operate, the caking precipitation of excrement can be reduced, stink when reducing the interference of residue, and reducing detection is easy to from clearly
The parasitic ovum in excrement is observed in the visual field, improves recall rate.
The invention further relates to utilize parasitic ovum in the Sample storage liquid detection excrement for parasitic ovum detection
Method, the method includes：
(1) 2~3g fecal specimens are placed in the 8~16mL of Sample storage liquid for parasitic ovum detection, are obtained
To mixture；
(2) by step (1) mixture mixing, 65~80 mesh sieve (preferably 70 mesh) is crossed, filtrate is obtained；
(3) step (2) filtrate is transferred in bottle and (is preferably drop by drop squeezed out), be inverted 15~20min (preferably
15min)；
(4) inverted bottleneck takes a drop solution (preferably taking the first drop filtrate) from step (3), drops on glass slide, lid
Upper coverslip carries out observation detection under microscope.
Preferably, fecal specimens 2g in step (1), the Sample storage liquid dosage for parasitic ovum detection are 10mL.
The beneficial effects are mainly as follows：(1) present invention, which preserves liquid, can be applicable in the preservation of humans and animals excrement
And processing, cost is relatively low, in the case that keep parasitic ovum it is complete preferably solve sample can long-term preservation and convenient for fortune
It is defeated, improve environment when detection, the form for the parasitic ovum being stored in liquid basal cell treatment fluid of the present invention is made to have obtained well
Fixation and holding, avoid the swelling rupture and atrophy of parasitic ovum, convenient for diagnosis.(2) present invention is detected for parasitic ovum
Sample storage liquid reduce the component of visual field fatigue containing keeping visual field background soft in component, convenient for observing.
(4) it illustrates
Fig. 1 is the excrement that the Sample storage liquid for parasitic ovum detection through being obtained in embodiment in the present invention 1 detects people
The parasitic ovum photo that toilet contains.
Fig. 2 is the excrement that the Sample storage liquid for parasitic ovum detection through being obtained in embodiment in the present invention 2 detects duck
The parasitic ovum photo that toilet contains.
Fig. 3 is the excrement that the Sample storage liquid for parasitic ovum detection through being obtained in embodiment in the present invention 3 detects duck
The parasitic ovum photo that toilet contains.
(5) specific implementation mode
With reference to specific embodiment, the present invention is described further, but protection scope of the present invention is not limited in
This：
Embodiment 1：The preparation of Sample storage liquid for parasitic ovum detection
It is formulated for the Sample storage liquid of parasitic ovum detection by following formula composition, is formed per 1000mL as follows：
Paraformaldehyde：50g；
Methanol：15mL；
Ethyl alcohol：3 0mL；
L-Alanine：0.5g；
L-Isoleucine：0.5g；
Phosphate buffer：0.01mol/L；
Lauryl sodium sulfate：0.15g；
Vanillic aldehyde：7.5g；
Malachite green：0.07g；
Glycerine：5g；
Surplus is water, adjusts pH value to 6.7.
Utilize the method for parasitic ovum in the Sample storage liquid detection human faecal mass for parasitic ovum detection, the side
Method includes：
(1) 2g fecal specimens are placed in the Sample storage liquid 10mL for parasitic ovum detection, are mixed
Object；
(2) by step (1) mixture mixing, 70 mesh sieve is crossed, filtrate is obtained；
(3) step (2) filtrate is drop by drop expressed into bottle, is inverted 15min；
(4) inverted bottleneck takes a drop solution (taking the first drop filtrate) from step (3), drops on glass slide, closes the lid
Slide carries out observation detection under microscope.
Fig. 1 is the excrement of the Sample storage liquid processing people for parasitic ovum detection through the present embodiment, detected
Pinworm worm's ovum, clear background in figure, worm's ovum is intact, is easy to observe, convenient for being used for clinical detection.
Embodiment 2：The preparation of Sample storage liquid for parasitic ovum detection
It is formulated for the Sample storage liquid of parasitic ovum detection by following formula composition, is formed per 1000mL as follows：
Paraformaldehyde：80g；
Methanol：30mL；
Ethyl alcohol：30mL；
L-Alanine：0.6g；
L-Isoleucine：0.6g；
Phosphate buffer：0.012mol/L；
Lauryl sodium sulfate：0.2g；
Vanillic aldehyde：8g；
Malachite green：0.08g；
Glycerine：30g；
Surplus is water, adjusts pH value to 6.4.
Fig. 2 is the excrement (detection method of the Sample storage liquid processing animal for parasitic ovum detection through the present embodiment
With embodiment 1), detected worm's ovum, clear background in figure, worm's ovum preserves complete, is uniformly dispersed, is easy to observe, convenient for using
In clinical detection.
Embodiment 3：The preparation of Sample storage liquid for parasitic ovum detection
It is formulated for the Sample storage liquid of parasitic ovum detection by following formula composition, is formed per 1000mL as follows：
Paraformaldehyde：70g；
Methanol：2 0mL；
Ethyl alcohol：20mL；
L-Alanine：0.4g；
L-Isoleucine：0.4g；
Phosphate buffer：0.009mol/L；
Lauryl sodium sulfate：0.12g；
Vanillic aldehyde：5g；
Malachite green：0.07g；
Glycerine：40g；
Surplus is water, adjusts pH value to 6.5.
Fig. 3 is the excrement (detection method of the Sample storage liquid processing animal for parasitic ovum detection through the present embodiment
With embodiment 1), detected worm's ovum, clear background in figure, worm's ovum form remains good, is easy to observe, be uniformly dispersed, just
In for clinical detection.
Claims (4)
1. a kind of Sample storage liquid for parasitic ovum detection includes mainly following components：
50~100g/L of paraformaldehyde, 10~30mL/L of methanol, 10~50mL/L of ethyl alcohol, 0.3~0.8g/L of l-Alanine, L- are different
0.3~0.8g/L of leucine, 0.005~0.015mol/L of phosphate buffer, 0.1~0.3g/L of lauryl sodium sulfate are fragrant
Blue element 5~10g/L, 0.05~0.09g/L of malachite green, 30~80g/L of glycerine, adjust pH 6.2~6.8, surplus is water.
2. the Sample storage liquid for parasitic ovum detection as described in claim 1, it is characterised in that its composition is as follows：
60~80g/L of paraformaldehyde, 20~25mL/L of methanol, 20~30mL/L of ethyl alcohol, 0.5~0.6g/L of l-Alanine, L- are different
0.4~0.6g/L of leucine, 0.008~0.01mol/L of phosphate buffer, 0.15~0.2g/L of lauryl sodium sulfate are fragrant
Blue element 6~7g/L, 0.06~0.07g/L of malachite green, 50~60g/L of glycerine, adjust pH 6.5, surplus is water.
3. the method for parasitic ovum in the Sample storage liquid detection excrement of parasitic ovum detection described in claim 1 is utilized,
The method includes：
(1) 2~3g fecal specimens are placed in the 8~16mL of Sample storage liquid for parasitic ovum detection, are mixed
Close object；
(2) by step (1) mixture mixing, 65~80 mesh sieve is crossed, filtrate is obtained；
(3) step (2) filtrate is transferred in bottle, is inverted 15~20min；
(4) inverted bottleneck takes a drop solution from step (3), drops on glass slide, covered, is seen under microscope
Examine detection.
4. method as claimed in claim 3, it is characterised in that fecal specimens 2g in step (1), for parasitic ovum detection
Sample storage liquid dosage is 10mL.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711433166.2A CN108308172A (en) | 2017-12-26 | 2017-12-26 | A kind of Sample storage liquid for parasitic ovum detection |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711433166.2A CN108308172A (en) | 2017-12-26 | 2017-12-26 | A kind of Sample storage liquid for parasitic ovum detection |
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| CN108308172A true CN108308172A (en) | 2018-07-24 |
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| CN201711433166.2A Pending CN108308172A (en) | 2017-12-26 | 2017-12-26 | A kind of Sample storage liquid for parasitic ovum detection |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108844800A (en) * | 2018-09-10 | 2018-11-20 | 江西业力医疗器械有限公司 | A kind of disposable excrement parasitic ovum acquisition pelletizer |
| CN111937855A (en) * | 2019-05-15 | 2020-11-17 | 博朗（厦门）生物科技有限公司 | Parasite egg preservation reagent, application and parasite egg detection method |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1861787A (en) * | 2005-05-12 | 2006-11-15 | 徐朝斌 | Cell dispersion treating agent |
| CN104480012A (en) * | 2014-11-27 | 2015-04-01 | 南方医科大学 | Preserving liquid for faecal bacteria |
| CN105385599A (en) * | 2016-01-21 | 2016-03-09 | 南京医科大学第一附属医院 | Coprophilous fungus preservation solution and method for preserving coprophilous fungi |
| CN106596211A (en) * | 2015-10-15 | 2017-04-26 | 深圳华大基因研究院 | Stool sample preservation liquid, preparation method and application thereof |
| CN106769289A (en) * | 2016-12-02 | 2017-05-31 | 刘鹏飞 | A kind of collocation method of fecal specimens storage liquid and application |
| CN107312717A (en) * | 2017-08-08 | 2017-11-03 | 苏州阿迪康生物科技有限公司 | A kind of store method of excrement class sample, preservation solution, preparation method and application |
-
2017
- 2017-12-26 CN CN201711433166.2A patent/CN108308172A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1861787A (en) * | 2005-05-12 | 2006-11-15 | 徐朝斌 | Cell dispersion treating agent |
| CN104480012A (en) * | 2014-11-27 | 2015-04-01 | 南方医科大学 | Preserving liquid for faecal bacteria |
| CN106596211A (en) * | 2015-10-15 | 2017-04-26 | 深圳华大基因研究院 | Stool sample preservation liquid, preparation method and application thereof |
| CN105385599A (en) * | 2016-01-21 | 2016-03-09 | 南京医科大学第一附属医院 | Coprophilous fungus preservation solution and method for preserving coprophilous fungi |
| CN106769289A (en) * | 2016-12-02 | 2017-05-31 | 刘鹏飞 | A kind of collocation method of fecal specimens storage liquid and application |
| CN107312717A (en) * | 2017-08-08 | 2017-11-03 | 苏州阿迪康生物科技有限公司 | A kind of store method of excrement class sample, preservation solution, preparation method and application |
Non-Patent Citations (4)
| Title |
|---|
| BONNIE L WEBSTER: "Isolation and preservation of schistosome eggs and larvae inRNAlater facilitates genetic profiling of individuals", 《PARASITES & VECTORS》 * |
| CRAWLEY ET AL: "Testing storage methods of faecal samples for subsequent measurement of helminth egg numbers in the domestic horse", 《VETERINARY PARASITOLOGY》 * |
| STRONA G: "Field preservation of monogenean parasites for molecular and morphological analyses", 《PARASITOLOGY INTERNATIONAL》 * |
| 曾庆其: "长时间保存虫卵的方法", 《实用医学杂志》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108844800A (en) * | 2018-09-10 | 2018-11-20 | 江西业力医疗器械有限公司 | A kind of disposable excrement parasitic ovum acquisition pelletizer |
| CN108844800B (en) * | 2018-09-10 | 2023-11-03 | 广州市准好生物科技有限公司 | Disposable parasitic insect egg of excrement gathers film-making device |
| CN111937855A (en) * | 2019-05-15 | 2020-11-17 | 博朗（厦门）生物科技有限公司 | Parasite egg preservation reagent, application and parasite egg detection method |
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Application publication date: 20180724 |