CN1861780A - Microorganism keeping living process - Google Patents
Microorganism keeping living process Download PDFInfo
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- CN1861780A CN1861780A CN 200510034500 CN200510034500A CN1861780A CN 1861780 A CN1861780 A CN 1861780A CN 200510034500 CN200510034500 CN 200510034500 CN 200510034500 A CN200510034500 A CN 200510034500A CN 1861780 A CN1861780 A CN 1861780A
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- microorganism
- bacterium
- bacteria
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- photosynthetic bacterium
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Abstract
This invention discloses a keep-alive method of microorganism, concretely speaking it is the protection method of the concentrated or no concentrated microorganism by the concentrated photosynthetic bacterium. This method can elongate the keep-live time of the microorganism, and it is good to the microorganism, especially it is good to the application of the environment beneficial microorganism. This invention can be realized by the technology scheme as fellows: the concentrated photosynthetic bacterium liquid the viable count of which is above 20 billion/ ml is mixed with the ready protection of concentrated or no concentrated microorganism fermentation or culture liquid, and the ratio of them is 0.01-10000:1. The technology of this method is simple, and the cost of protection is low.
Description
Technical field
The present invention relates to a kind of microorganism keeping living process, is to utilize concentrating photosynthetic bacteria to the method that concentrates or non-spissated microbial preparation carries out keep-alive specifically.
Background technology
Along with people are more and more deep to environment beneficial microorganism understanding, the environment beneficial microorganism obtains application more and more widely, and to the Sustainable development aspect of protection breeding environment and aquaculture industry, the environment beneficial microorganism uses very important meaning.
Different with in the past preparation microbial strains and microbial metabolites, the environment beneficial microorganism requires to add the microorganism of the work that can breed of sufficient amount in specific environment for use.That is to say, both required enough bacterial counts, require to keep active again, and can not make use cost too high.Therefore, how the environment beneficial microorganism is carried out keep-alive, extend the shelf life, the popularizations of these microorganisms is had extremely important meaning.
Conventional microorganism keepalive method such as lyophilization, succeeding transfer culture etc. not can satisfy the service requirements of environmental microorganism because biomass is too little.
Summary of the invention
The purpose of this invention is to provide a kind of microorganism keeping living process, a kind of method that prolongs the microbial preparation keep-alive phase.
The photosynthetic bacterium viable count is the photosynthetic bacterium concentrated solution more than 20,000,000,000/milliliter, is a kind of gelatinous liquid preparation of being with.Said preparation in the following aspects applicable to the live microorganism keep-alive: at first; the similar a kind of embedding medium of said preparation; can reduce the exercise performance of bacterium, can fix other microbial cells to a certain extent, can protect bacterium not polymerization precipitation and inhibition cracking inhibition mutually mutually.Secondly, the photosynthetic bacterium of high density has the restraining effect to the putrefactive bacterium breeding, can protect other microbial preparation that corruption does not take place.Once more, photosynthetic bacterium self can also produce benefit materials such as a large amount of VITAMIN and other nutritive substance, can keep other microbic activity under normal temperature and liquid state, and prolong its keep-alive phase.
The present invention utilizes the concentrating photosynthetic bacteria preparation to other microorganism keeping living effect, is achieved through the following technical solutions:
Is the photosynthetic bacterium viable count photosynthetic bacterium concentrated solution more than 20,000,000,000/milliliter; preferred concentration is more than 50,000,000,000/milliliter; more preferably concentration is more than 1,000 hundred million/milliliter, with treat keep-alive concentrate or non-spissated microbial fermentation solution, nutrient solution or preparation according to 0.01-10000: 1 mixed gets final product.
Microorganism of the present invention comprises: budding fungus; Ray fungi; Various bacteriums such as milk-acid bacteria, nitrogen-fixing bacteria, acetic bacteria, sulphate reducing bacteria, nitrobacteria, denitrifying bacterium, bacillus thuringiensis, xanthan bacterium, phosphate-solubilizing bacteria, potassium bacterium, bacteriophagic Bdellovibrio, genus bacillus; Various fungies such as aspergillus niger; Various unicellular algaes such as blue-green algaes such as diatoms such as green algas such as chlorella, Skeletonema Greville, chrysophyceae, spirulina, Microcystis aeruginosa, Euglena, haematococcus pulvialis, salt algae.The present method of can using of the microorganism that the present invention does not enumerate prolongs its keep-alive phase.
But utilize method of the present invention can prepare stable preservation, the compound formulation of forming by concentrating photosynthetic bacteria and other beneficial microorganism.And photosynthetic bacterium itself is a kind of common environment beneficial microorganism, and these preparations can be widely used in industries such as environment protection, aquaculture, breed and plantation.
Use photosynthetic bacterium keep-alive microorganism, not only can reduce the use of chemical additive, and can bring into play photosynthetic bacterium simultaneously and, can play the effect of associating use by the function of keep-alive bacterium.
Embodiment
Application example one:
Candida tropicalis concentrated solution, bacteria content are 3,000,000,000/milliliter, are that the concentrating photosynthetic bacteria of 2,000 hundred million/milliliter mixes preservation at 1: 1 with bacterial count, and after 6 months, the yeast bacterial count of living is 1,500,000,000/milliliter, photosynthetic bacterium live bacterial count be 1,000 hundred million/milliliter.Simultaneously, do not add the yeast concentrated solution of anything with it,, corruption takes place, do not examine yeast bacterium alive by infectation of bacteria.
Application example two:
The chlorella concentrated solution, the algae content of living is 2,000,000,000/milliliter, is that the concentrating photosynthetic bacteria of 2,000 hundred million/milliliter mixes preservation at 4: 1 with bacterial count, after 2 months, the chlorella algae number of living is 1,500,000,000/milliliter, photosynthetic bacterium live bacterial count be 40,000,000,000/milliliter.Simultaneously, do not add the chlorella concentrated solution of anything with it, corrupt smelly after week, do not examine the chlorella viable cell.
Application example three:
Milk-acid bacteria concentrated solution, bacteria content are 10,000,000,000/milliliter, are that the concentrating photosynthetic bacteria of 2,000 hundred million/milliliter mixes preservation at 3: 1 with bacterial count, and after 12 months, the milk-acid bacteria bacterial count of living is 2,000,000,000/milliliter, photosynthetic bacterium live bacterial count be 50,000,000,000/milliliter.Simultaneously, do not add the milk-acid bacteria concentrated solution of anything with it, the bacterial count of living is 1,000 ten thousand/milliliter.
Claims (4)
1, a kind of microorganism keeping living process; it is characterized in that: is the photosynthetic bacterium viable count photosynthetic bacterium concentrated solution more than 20,000,000,000/milliliter, with treat keep-alive concentrate or non-spissated microbial fermentation solution, nutrient solution or preparation according to 0.01-10000: 1 mixed is also preserved.
2, a kind of microorganism keeping living process according to claim 1 is characterized in that described microorganism is meant: budding fungus; Ray fungi; Various bacteriums such as milk-acid bacteria, nitrogen-fixing bacteria, acetic bacteria, sulphate reducing bacteria, nitrobacteria, denitrifying bacterium, bacillus thuringiensis, xanthan bacterium, phosphate-solubilizing bacteria, potassium bacterium, bacteriophagic Bdellovibrio, genus bacillus; Various fungies such as aspergillus niger; Various unicellular algaes such as blue-green algaes such as diatoms such as green algas such as chlorella, Skeletonema Greville, chrysophyceae, spirulina, Microcystis aeruginosa, Euglena, haematococcus pulvialis, salt algae.
3, a kind of microorganism keeping living process according to claim 1 is characterized in that described photosynthetic bacterium viable count concentration is more than 50,000,000,000/milliliter.
4,, it is characterized in that described photosynthetic bacterium viable count is more than 1,000 hundred million/milliliter according to claim 1 or 3 described a kind of microorganism keeping living process.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510034500 CN1861780A (en) | 2005-05-11 | 2005-05-11 | Microorganism keeping living process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510034500 CN1861780A (en) | 2005-05-11 | 2005-05-11 | Microorganism keeping living process |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1861780A true CN1861780A (en) | 2006-11-15 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200510034500 Pending CN1861780A (en) | 2005-05-11 | 2005-05-11 | Microorganism keeping living process |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107743840A (en) * | 2017-11-24 | 2018-03-02 | 桂林满梓玉农业开发有限公司 | A kind of cultural method of Asiatic plantain |
-
2005
- 2005-05-11 CN CN 200510034500 patent/CN1861780A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107743840A (en) * | 2017-11-24 | 2018-03-02 | 桂林满梓玉农业开发有限公司 | A kind of cultural method of Asiatic plantain |
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