CN1836491A - Method for promoting exercise survival rate of Prunus maritime cell engineered seedling - Google Patents
Method for promoting exercise survival rate of Prunus maritime cell engineered seedling Download PDFInfo
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- CN1836491A CN1836491A CN200610039337.9A CN200610039337A CN1836491A CN 1836491 A CN1836491 A CN 1836491A CN 200610039337 A CN200610039337 A CN 200610039337A CN 1836491 A CN1836491 A CN 1836491A
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- seedling
- cell engineering
- shore
- plum
- survival rate
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- 230000004083 survival effect Effects 0.000 title claims abstract description 13
- 235000003840 Amygdalus nana Nutrition 0.000 title claims abstract description 5
- 241000220299 Prunus Species 0.000 title claims abstract description 5
- 235000011432 Prunus Nutrition 0.000 title claims abstract description 5
- 235000014774 prunus Nutrition 0.000 title claims abstract description 5
- 238000000034 method Methods 0.000 title claims description 18
- 230000001737 promoting effect Effects 0.000 title 1
- 240000007322 Prunus maritima Species 0.000 claims description 25
- 235000015571 Prunus maritima Nutrition 0.000 claims description 21
- 241000233866 Fungi Species 0.000 claims description 10
- 239000002068 microbial inoculum Substances 0.000 claims description 9
- 241000894006 Bacteria Species 0.000 claims description 5
- 238000011156 evaluation Methods 0.000 claims description 3
- 235000015097 nutrients Nutrition 0.000 claims description 3
- 238000010908 decantation Methods 0.000 claims description 2
- 238000012216 screening Methods 0.000 claims description 2
- 238000001914 filtration Methods 0.000 claims 1
- 241000196324 Embryophyta Species 0.000 abstract description 21
- 238000005516 engineering process Methods 0.000 abstract description 9
- 238000010521 absorption reaction Methods 0.000 abstract description 6
- 230000003716 rejuvenation Effects 0.000 abstract description 2
- 238000012258 culturing Methods 0.000 abstract 1
- 230000008635 plant growth Effects 0.000 abstract 1
- 230000007704 transition Effects 0.000 description 6
- 230000008569 process Effects 0.000 description 5
- 238000007670 refining Methods 0.000 description 5
- 238000010899 nucleation Methods 0.000 description 4
- 230000000243 photosynthetic effect Effects 0.000 description 4
- 239000011159 matrix material Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 244000037666 field crops Species 0.000 description 2
- 231100000225 lethality Toxicity 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- 241000317178 Claroideoglomus etunicatum Species 0.000 description 1
- 206010017533 Fungal infection Diseases 0.000 description 1
- 208000031888 Mycoses Diseases 0.000 description 1
- 241000219793 Trifolium Species 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000032696 parturition Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
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- Cultivation Of Plants (AREA)
Abstract
The present invention belongs to the field of plant cell engineering seedling culturing technology. The technology of the present invention can raise the absorption capacity of roots of Prunus martima cell engineering seedling, raise the survival rate of Prunus martima cell engineering seedling in hardening off, and lay foundation for the survival, fast rejuvenation and further growth of plant.
Description
One, technical field
This invention belongs to plant cell engineering seedling culture technique field.
Two, background technology
The shore plum is a kind of husky salt tolerant fruit shrubs of giving birth to, originate in the U.S., Atlantic bank, from the Newfoundland to North Carolina Na Zhou distribution is arranged all, it can adapt to existence in unstable matrix and highly-saline environment, so it is one of important tool kind of a kind of beach degraded ecosystem restoration of the ecosystem.Admire that by the doctoral advisor of biotechnology research institute of Nanjing University professor introduces a fine variety it of China, and expand numerous by the cell engineering clone technology.Just trained the absorption function of its root of woody plant cell engineering Seedling of producing the chamber from group, the transporting function difference of stem and the photosynthetic capacity of leaf are low, before the field cultivating of field, must pass through this significant process of hardening from group training chamber, and traditional hardening off method normally passes through indoor " opening bottleneck " indoor transition earlier, the booth high humidity, seedling is practiced in low light level cultivation transition, and then outdoor planting, but the lethality of woody plant cell engineering Seedling is still very high, this problem fails to be well solved for a long time, be difficult to really bring into play the advantage of plant cell engineering seedling, hindered the extensive promotion and application of this clone's raising technology of plant cell engineering seedling.This is that the basic absorption function that solves just the woody plant cell engineering shoot root that comes out from media transfer is poor, the seedling body is very delicate, the problem of bad adaptability because traditional hardening off method is failed.Therefore, traditional hardening off method very easily makes the woody plant cell engineering Seedling occur withering or rots, and lethality is very high.
Three, summary of the invention
(1) the present invention's problem that need solve is: the absorption function transporting function poor, stem that overcomes shore plum cell engineering shoot root is weak, the problem that photosynthetic capacity is low and the more delicate very easily dehydration of seedling body withers of leaf.
(2) concrete technical scheme of the present invention is:
We are poor at the absorption function of the feature of shore plum and biological property and woody cell engineering shoot root, a little less than the transporting function of stem, these characteristics that the photosynthetic capacity of leaf is low, invented the absorbing capacity that in the plum refining seeding in cell engineering process of shore, improves its root, for the g and D of plant provides good moisture and nutrition (nitrogen particularly, P elements) environmental condition, strengthen root in the plum refining seeding in cell engineering process of shore, stem, growing and rejuvenation of leaf, improve premunition and adaptive capacity in the plum refining seeding in cell engineering process of shore, improve its survival rate greatly, lay a solid foundation for the plant cell engineering seedling can large batch ofly move towards the land for growing field crops from the laboratory.
Concrete technical scheme is as follows:
1. the dendritic mycorhiza of shore, different habitat plum plant rhizosphere clump (being called for short the AM mycorhiza) is collected, is identified by fungi;
2. blazoning mycorhiza bacterium purifying, expand numerous microbial inoculum of making above-mentioned investigation;
3. infect shore plum cell engineering Seedling with the microbial inoculum of making;
4. shore plum cell engineering Seedling AM mycorhiza bacterium is infected check;
5. management: carry out managing with condition with control group;
6. observe and statistics: the shore plum cell engineering Seedling and the control group that will infect AM mycorhiza bacterium compare, and observe its growth potential and add up survival rate.
(3) the present invention compared with the prior art, its significance progress is:
Hardening is the key link comparatively in the woody plant cell engineering Seedling culture technique, and can it be directly connected to cell engineering Seedling and survive and be applied to as soon as possible the land for growing field crops and go in producing.And traditional hardening off method mostly just adopts earlier through indoor " uncork hardening " transition, booth high humidity, low light level cultivation transition, and then the passive hardening process of outdoor planting, fundamentally do not solve the 1. 2. 3. low problem of photosynthetic capacity of leaf a little less than the transporting function of stem of absorption function difference of root of woody plant cell engineering Seedling.The present invention utilizes the method for the dendritic mycorhiza of shore plum plant rhizosphere clump (being called for short the AM mycorhiza) fungal infection shore plum cell engineering Seedling, improve the absorbing capacity of its root to liquid manure, strengthen the anti-adversity ability of self, make plant healthy and strong more, fundamentally improved the various habitats of the adaptation ability of plant, helped survival rate and improve and growth and development of plant.Guarantee that the plant cell engineering seedling tides over the hardening phase smoothly.Use the survival rate of the shore plum cell engineering Seedling of AM mycorrhizal fungi dip-dye to improve 35%, improve the survival rate of the process of its hardening greatly than control group.
The present invention can increase substantially the survival rate in the plant cell engineering seedling refining process, and the large tracts of land of this no sex clone raising technology of shore plum woody plant cell engineering Seedling is promoted provides reliable guarantee.The real plant cell engineering seedling of realizing can expand numerous advantage fast, has also reduced the cost of producing the plant cell engineering seedling simultaneously, for the practical application of this quick multiplication technique provides wide prospect.
Still there are not plum method for refining seeding in cell engineering research of this shore and relevant report at present both at home and abroad.
Four, embodiment
1. the dendritic mycorhiza of shore plum plant rhizosphere clump (being called for short the AM mycorhiza) fungi is collected, identify: breed the base from Yancheng Da Feng solonchak of shore, Nanjing University halophytes laboratory plum, Pukou is bred base and Lishui and is bred and choose the above shore plum plant of life in 2 years in the base and gather the radicula soil of shore plum and mark (about the degree of depth that fetches earth 15cm, contain the thinnest lateral root), take each sample to laboratory and carry out spore and the sporocarp that the wet screening decantation is collected arbuscular mycorrhizal fungi, the parallel evaluation [with reference to the key of Trappe (1982), " the VA mycorrhizal fungi identification handbook " of Schenck etc. (1988) establishment, the current paper data of also publishing with reference to relevant publication uses common morphological feature sorting technique to identify simultaneously].
2. blazoning mycorrhizal fungi (" children cover sacculus mould Glomus etunicatum ") purifying and expanding numerous microbial inoculum of making above-mentioned evaluation with clover;
3. the shore plum cell engineering Seedling process that will grow good, that well developed root system is relatively more healthy and stronger was transferred to and is carried out booth high humidity, low light level cultivation transition white silk seedling in the matrix that contains the AM microbial inoculum again after " opening bottleneck " indoor transition 5-7 days;
4. management: make and infect 2. that the shore plum cell engineering Seedling of microbial inoculum is under the identical management condition with control group, the temperature of note to keep practicing seedling room is the condition hardening at 75-90% in temperature 26-28 ℃ of daytime, temperature 14-16 ℃ of night, humidity, waters experimental group and control group at the hardening initial stage with 0.5 times nutrient solution (seeing Table 1);
5. shore plum cell engineering Seedling AM mycorhiza is contaminated check: shore plum cell engineering Seedling contain the AM microbial inoculum matrix in practice seedling after 60 days, carry out sampling inspection, the infection rate of check shore plum cell engineering Seedling;
6. observe and statistics: practice seedling after 60 days, the experimental group shore plum cell engineering Seedling of contaminating the Cong Zhizhuan mycorrhizal fungi is compared with the control group shore plum cell engineering Seedling of not contaminating the AM mycorhiza, good and the survival rate of the growth potential of experimental group is 86% as a result, the survival rate of control group is 51%, exceeds control group 35%.
Table 1 nutrient composition proportioning
| Composition | Dosage (g/L) | Composition | Dosage (g/L) | ||
| 1 | KH2PO 4 | 0.14 | 7 | Ironic citrate | 0.02 |
| 2 | KNO 3 | 0.51 | 8 | ZnSO 4·7H 2O | 0.22 |
| 3 | Ca(NO 3) 2 | 0.82 | 9 | CuSO 4·5H 2O | 0.08 |
| 4 | MgSO 4 | 0.49 | 10 | H 2MO 4·H 2O | 0.02 |
| 5 | H 3BO 3 | 2.86 | |||
| 6 | MnCl 2·4H 2O | 1.81 |
Claims (1)
1, a kind of method that improves exercise survival rate of Prunus maritime cell engineered seedling is characterized in that:
1. the AM mycorrhizal fungi is collected, identifies: the shore plum plant rhizosphere from different habitats is gathered the native also mark of radicula of shore plum (about the degree of depth that fetches earth 15cm, contain the thinnest lateral root), take each sample to laboratory and carry out spore and the sporocarp that the wet screening decantation is collected arbuscular mycorrhizal fungi, the parallel evaluation;
2. filtering out the type that the blazons mycorhiza bacterium that all has in several habitats in the AM mycorrhizal fungi of 1. collecting carries out purifying, expands numerous microbial inoculum of making;
3. infect shore plum cell engineering Seedling with the microbial inoculum of 2. making and make it to form the AM mycorhiza;
4. management: shore plum cell engineering Seedling and control group that 2. microbial inoculum is infected carry out managing with condition, place it in temperature in temperature 26-28 ℃ of daytime, temperature 14-16 ℃ of night, humidity the condition hardening at 75-90%, nutrient solution pouring experimental group and control group at the hardening initial stage with 0.5 times;
5. shore plum cell engineering Seedling AM mycorhiza bacterium is infected check.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200610039337.9A CN1836491A (en) | 2006-04-06 | 2006-04-06 | Method for promoting exercise survival rate of Prunus maritime cell engineered seedling |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200610039337.9A CN1836491A (en) | 2006-04-06 | 2006-04-06 | Method for promoting exercise survival rate of Prunus maritime cell engineered seedling |
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| Publication Number | Publication Date |
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| CN1836491A true CN1836491A (en) | 2006-09-27 |
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| CN200610039337.9A Pending CN1836491A (en) | 2006-04-06 | 2006-04-06 | Method for promoting exercise survival rate of Prunus maritime cell engineered seedling |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101874447B (en) * | 2009-11-17 | 2012-01-25 | 金陵科技学院 | Beach plum root cutting seedling method |
| CN102771350A (en) * | 2012-07-10 | 2012-11-14 | 青岛农业大学 | Method for cultivating mycorrhizal seedlings |
| CN103718908A (en) * | 2014-01-14 | 2014-04-16 | 金陵科技学院 | Method for cultivating seedlings of prunus maritime root suckers |
| CN105532270A (en) * | 2016-01-25 | 2016-05-04 | 金陵科技学院 | Beach plum scion grafting seedling culture method |
-
2006
- 2006-04-06 CN CN200610039337.9A patent/CN1836491A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101874447B (en) * | 2009-11-17 | 2012-01-25 | 金陵科技学院 | Beach plum root cutting seedling method |
| CN102771350A (en) * | 2012-07-10 | 2012-11-14 | 青岛农业大学 | Method for cultivating mycorrhizal seedlings |
| CN102771350B (en) * | 2012-07-10 | 2014-11-05 | 青岛农业大学 | Method for cultivating mycorrhizal seedlings |
| CN103718908A (en) * | 2014-01-14 | 2014-04-16 | 金陵科技学院 | Method for cultivating seedlings of prunus maritime root suckers |
| CN103718908B (en) * | 2014-01-14 | 2015-03-04 | 金陵科技学院 | Method for cultivating seedlings of prunus maritime root suckers |
| CN105532270A (en) * | 2016-01-25 | 2016-05-04 | 金陵科技学院 | Beach plum scion grafting seedling culture method |
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