CN111484950A - Phosphate solubilizing bacillus and application thereof - Google Patents
Phosphate solubilizing bacillus and application thereof Download PDFInfo
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
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- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention provides a phosphate-solubilizing bacillus and application thereof, wherein the bacillus is bacillus subtilis FJAT-10275 which is preserved in China general microbiological culture Collection center with the preservation number of CGMCC NO. 16327. The bacillus of the invention can effectively degrade inorganic phosphorus, improve the content of soluble phosphorus in soil, obviously promote the growth of crops and enhance the stress resistance of the crops.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and particularly relates to a phosphate solubilizing bacillus strain and application thereof in plant growth.
Background
The phosphorus supply level of soil is one of the key factors influencing the growth of plants, 95 percent of phosphorus in the soil is in an invalid form, and the plants are difficult to directly absorb and utilize, so that the phosphorus deficiency phenomenon exists in 74 percent of cultivated land soil in China.
In a crop-microorganism interaction system, Plant growth-promoting rhizobacteria (PGPR) are colonized in the rhizosphere soil of crops, and can effectively decompose insoluble and fixed elements (phosphorus, potassium and the like) in the soil, promote the absorption of the crops on fertilizers and elements in the soil, and further promote the growth, yield increase, disease resistance and the like of the crops. Therefore, the microbial fertilizer with the efficient growth promoting function is screened and developed and applied to agricultural production, potential element resources of soil are fully utilized, and the microbial fertilizer has important significance for improving element shortage of soil such as phosphorus and potassium and the like, reducing environmental pollution and promoting agricultural sustainable development.
Disclosure of Invention
Therefore, a strain capable of promoting growth and dissolving phosphorus is needed to be provided, and the problem that elements such as phosphorus, potassium and the like in soil cannot be absorbed and utilized by plants is solved.
In order to achieve the purpose, the inventor provides the following technical scheme:
a bacillus phosphate solubilizing bacterium, comprising: the Bacillus is Bacillus subtilis FJAT-10275 with the scientific name of Bacillus subtilis subsp. inaquosum FJAT-10275, which is preserved in the common microorganism center of China Committee for culture Collection of microorganisms with the preservation number of CGMCC NO.16327, the preservation date of 2018, 8 and 21 days and the preservation address of the institute of microbiology, China academy of sciences, Beijing, China.
The colony morphology of the bacillus FJAT-10275 is as follows: the colony has wet and translucent surface, many folds and light white color.
Further, the application of the bacillus phosphate solubilizing bacteria in degradation of insoluble phosphate is provided.
The specific application method is that the bacillus fermentation liquor is prepared into the concentration of 1 × 105-2×105cfu/m L, soaking the strain for 2-3 days, and placing at 25-30 deg.C under illumination for 16-20 h/day.
A plant growth promoting microbial inoculum comprises the bacillus.
The invention has the beneficial effects that:
(1) the bacillus of the invention can effectively degrade inorganic phosphorus, promote insoluble phosphate to release phosphorus, and improve the content of soluble phosphorus in soil, thereby obviously promoting the growth of crops, enabling the roots of the crops to be developed, and enhancing the stress resistance of the crops.
(2) The bacillus of the invention can improve the activity of plant seeds, promote the seeds to take root and sprout, and effectively shorten the growth cycle of plants.
Drawings
FIG. 1 shows the colony morphology of Bacillus FJAT-10275 according to an embodiment.
FIG. 2 is a tree showing the results of identifying the 16S rRNA sequence of Bacillus FJAT-10275 according to an embodiment.
FIG. 3 shows the effect of Bacillus FJAT-10275 on the growth of tomato seeds according to an embodiment.
FIG. 4 shows the growth promoting effect of Bacillus FJAT-10275 on tomato seeds.
Detailed Description
To explain technical contents, achieved objects and effects of the technical solutions in detail, the following detailed description is given with reference to the accompanying drawings in combination with specific embodiments.
EXAMPLE 1 phosphate solubilization of Bacillus
1. Test materials
1.1 test strains
Test strains: the bacillus FJAT-10275 is isolated from leaves of Mandarin orange of Changchang Huanglongbing disease in Fujian province, is frozen and preserved in glycerol at-80 ℃ in the China general microbiological culture Collection center with the preservation number of CGMCC No. 16327.
1.2 culture Medium
L B solid culture medium (purchased from the manufacturer) formula, i.e. 10g tryptone, 10g sodium chloride, 5g yeast powder, 15g agar, 1000m L water, pH 7.0, (2) L B liquid culture medium (purchased from the manufacturer) formula, i.e. 10g tryptone, 10g sodium chloride, 5g yeast powder, 1000m L water, pH 7.0.
Inorganic phosphorus medium (NBRIP medium): glucose 10g, Ca3(PO4)25g，MgCl2·6H2O 5g，KCl0.2g，MgSO4·7H2O 0.25g，(NH4)2SO40.1g, distilled water 1000m L, natural pH.
The organic phosphorus growth medium comprises 10g of glucose, 0.5g of ammonium sulfate, 0.5g of yeast extract powder, 0.3g of sodium chloride, 0.3g of potassium chloride, 0.3g of magnesium sulfate, 0.03g of ferrous sulfate, 0.03g of manganese sulfate, 0.2g of lecithin, 1.0g of calcium carbonate, 1000m of distilled water L, 15g of agar and pH 7.0-7.5.
1.3 preparation of test reagents
153m L concentrated sulfuric acid (analytically pure, density 1.84g/m L) is measured, slowly added into 400m L distilled water, continuously stirred and cooled, 10g of ground ammonium molybdate is also weighed and poured into the solution, stirred and dissolved, 0.5% (5 g/L) of antimony potassium tartrate solution 100m L is added, after cooling, water is added to dilute the solution to 1000m L, the solution is shaken evenly and stored in a brown reagent bottle, and the stock solution contains 1% of ammonium molybdate and 2.75mo L/L of sulfuric acid.
The molybdenum-antimony color-developing agent is prepared by weighing 1.50g ascorbic acid and dissolving in 100m L molybdenum-antimony storage solution, wherein the solution has short effective period and is suitable for use.
5 mg/L phosphorus Standard solution 0.4394g of potassium dihydrogen phosphate (KH) dried at 50 deg.C2PO4Analytically pure), 100m L water, 5m L concentrated sulfuric acid (preservation), water to make the volume 1L, the concentration of phosphorus 100 mg/L, the solution can be preserved for a long time, the solution 5m L is absorbed into a 100m L volumetric flask, water to make the volume 5 mg/L phosphorus standard solution, the solution is not suitable for long-term preservation.
2. Test method
2.1 determination of phosphate solubilizing ability
2.1.1 activation of the test strains
Taking out the test strain from a refrigerator at the temperature of minus 80 ℃, after the test strain is warmed to room temperature, streaking the test strain in an ultra-clean bench into a L B solid agar medium plate, inversely placing the test strain in a biological incubator for 2 d.2d at the temperature of 30 ℃, observing the colony morphology, selecting a single colony for secondary streaking culture, ensuring that the activated colony morphology is single, selecting a proper amount of the single colony in a L B liquid medium, and performing shake culture at the temperature of 30 ℃ for 2d to obtain a seed solution.
2.1.2 liquid Shake flask fermentation
Diluting the seed liquid by 2 times, and detecting OD by an enzyme-labeling instrument600nmCombining with bacterial count under microscope, diluting properly, and adjusting bacterial density to 108cfu/m L (bacterial liquid OD diluted 2 times)600nmBetween 0.3 and 0.5), 200 μ L was inoculated into 50m L centrifuge tubes containing 10m L liquid culture medium of organophosphorus and inorganic phosphorus, respectively, and shake-cultured at 230rpm and 30 ℃ for 6d, and two replicates of each test bacterium were inoculated with 200 μ L sterile water as a control.
2.1.3 detection of effective phosphorus content in supernatant by MoSb antibody method
a. Preparation of supernatant
And centrifuging the fermentation liquor cultured for 6d at 1200rpm for 30min, taking supernatant, and discarding the precipitate.
b. Drawing of standard curve
Respectively and accurately sucking 0, 2, 4, 6, 8 and 10m L of 5 mg/L phosphorus standard solution into a 50m L volumetric flask, diluting the solution to a position with water with the total volume of about 3/5, adding 2 drops of 2, 6-dinitrophenol as an indicator, adjusting 50m L/L dilute sulfuric acid (or hydrochloric acid) and 10 percent sodium hydroxide until the solution is just yellowish, accurately adding 5m L molybdenum-antimony anti-color developing agent, shaking up, adding water to fix the volume to obtain a standard solution series with phosphorus contents of 0.0, 0.2, 0.4, 0.8 and 1.0 mg/L, respectively, shaking up, standing at room temperature of more than 15 ℃, measuring the absorbance at a wavelength of 700nm, taking the absorbance as a vertical coordinate and the phosphorus concentration (mg/L) as a horizontal coordinate, and drawing a standard curve.
c. Determination of available phosphorus content in supernatant
Transferring appropriate amount of supernatant into 50m L volumetric flask, diluting with water to total volume of about 3/5, adding 1-2 drops of dinitrophenol indicator, accurately adding 5m L molybdenum antimony anti-color developing agent, shaking, adding water to desired volume, standing at room temperature above 15 deg.C for 30min, and reading absorbance OD700nmThen, the corresponding phosphorus content is checked from the standard curve.
d. Calculating the effective dissolved phosphorus content in the supernatant
The effective phosphorus content p (mg/L) of the supernatant is × colorimetric volume of the supernatant, × times of division/total volume of fermentation liquor
Wherein, the concentration of the supernatant fluid is that the concentration of phosphorus mg/L is found from a standard curve;
the colorimetric volume is 50m L of constant volume;
dividing times are the total volume of the fermentation liquor/sampling volume.
Effective phosphorus-dissolving amount P (mg/L) ═ effective phosphorus content of strain supernatant-control supernatant phosphorus content
2.2 phospholytic bacteria morphology and 16s rRNA identification
And (3) the morphology of the phosphate solubilizing bacteria, namely inoculating the purified bacillus onto an L B plate by streaking, culturing at the constant temperature of 30 ℃ for 48h, and observing the characteristics of the size, the color, the edge uniformity, the wettability and the like of a bacterial colony after the bacterial colony grows out.
The molecular identification is that pure strains are inoculated to L B liquid culture medium, the shaking table is placed at 30 ℃, after the strains are cultured to a logarithmic phase, the genome DNA of the strain FJAT-10275 is extracted by adopting a Tris-saturated phenol method, 16S rRNA gene universal primers 27F and 1492R are adopted for PCR amplification, the PCR reaction program refers to the literature of Zhengxuefang and the like (Zhengxuefang, Liubo, Zhuyanqing, and the like, the screening and identification of the tomato bacterial wilt biocontrol bacillus is [ J ]. Chinese biological prevention and control institute, 2016,32(5):657 and 665.), the PCR products are sent to Shanghai Boshang biotechnology Limited for Sanger sequencing, EZBIOCou is used for completing sequence homology comparison, and the sequence is analyzed by MEGA 6.0.6 software and the development tree is constructed through the MEGA 6.0.6 software.
3. Test results
3.1 determination of phosphate solubilizing ability
The phosphate solubilizing ability of Bacillus FJAT-10275 on organic and inorganic phosphorus is shown in Table 1. Experimental results show that the bacillus FJAT-10275 has a relatively obvious phosphate solubilizing effect on inorganic phosphorus, and has a relatively poor phosphate solubilizing effect on organic phosphorus.
TABLE 1 phosphate solubilizing ability of Bacillus FJAT-10275
It can be seen that Bacillus FJAT-10275, a phosphate solubilizing (inorganic phosphate degrading) microorganism, promotes apatite Ca3(PO4)2The insoluble phosphate releases phosphorus, and the content of soluble phosphorus in the soil is improved, so that the growth of crops can be obviously promoted, the root system of the crops is developed, and the stress resistance of the crops is enhanced.
3.2 identification of the Strain
3.2.1 Strain morphology
The colony morphology of FJAT-10275 is: the colony has wet and translucent surface, many folds and light white color. The colony morphology is shown in FIG. 1.
Identification of 3.2.216S rRNA Gene
The nucleic acid sequence of the 16S rRNA gene of the strain FJAT-10275 is as follows:
TGCAGTCGAGCGGACAGATGGGAGCTTGCTCCCTGATGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATGGTTGTTTGAACCGCATGGTTCAAACATAAAAGGTGGCTTCGGCTACCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGTTGTTAGGGAAGAACAAGTACCGTTCGAATAGGGCGGTACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCTCTGACAATCCTAGAGATAGGACGTCCCCTTCGGGGGCAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGACCTGGGCTACACACGTGCTACAATGGACAGAACAAAGGGCAGCGAAACCGCGAGGTTAAGCCAATCCCACAAATCTGTTCTCAGTTCGGATCGCAGTCTGCAACTCGACTGCGTGAAGCTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCACGAGAGTTTGTAACACCCGAAGTCGGTGAGGTAACCTTTTAGGAGCCAGCCGCCGAAG(SEQ ID NO:1)
comparing the 16S rRNA gene sequence SEQ ID NO 1 of the strain FJAT-10275 with an EZBioCloud gene database, the strain FJAT-10275 has the closest genetic relationship with Bacillus subtilis sp. Downloading 16S rRNA gene sequences of strains with higher homology, carrying out comparative analysis, constructing a phylogenetic tree, and forming the phylogenetic tree as shown in figure 2 when a neighbor-Joining method is adopted and the Bootstrap value is 1000 times. In the constructed phylogenetic tree, the strain FJAT-10275 and Bacillus subtilis sp.
EXAMPLE 2 growth promoting action of Bacillus
1. Tomato growth promotion test method
Selecting single colony of test strain, inoculating into 250m L conical flask containing 100m L L B liquid culture, and culturing at 30 deg.C for 48 hr (counting bacteria under microscope, bacterial density is 10%8cfu/m L or more), the test bacteria are culturedThe yeast solution was diluted 1000 times and clear water was used as control (ck).
Selecting tomato seeds with relatively consistent growth vigor, placing the tomato seeds in a transparent culture box of 9cm with 2-3 layers of filter paper laid at the bottom, placing 15 mung bean seeds in a constant-temperature artificial climate box at 27 ℃, and illuminating for 16h and dark for 8 h. Tracking and observing the tomato germination condition, recording the germination number until no new germination grains appear in 3d continuously, measuring the germ length of the tomato germination radicle machine, and analyzing the promoting effect of the test bacteria on tomato seed germination.
The germination percentage (number of germinated seeds on specified days/number of test seeds) is × 100%
The germination index is ∑ (Gt/Dt), wherein Gt is the number of seeds to be germinated at t d, and Dt is the corresponding number of days to be germinated.
Vigor index (germination index × embryo root length (cm)
The test data adopts DPS software and a new double-polarization method (Duncan) to carry out the significance test of the data difference among treatments.
2. Test results
The test group is compared with the ck group, see table 2, figure 3 and figure 4. the experimental result shows that the bacillus FJAT-10275 is diluted 1000 times, namely the concentration is about 1 × 10 compared with the growth effect of the tomato seeds of the ck control group5-2×105The germination rate, the germination index, the root length, the stem length and the seed vitality index of cfu/m L are 107.90%, 89.07%, 293.84%, 129.38% and 261.66% of ck control respectively, and the five indexes have significant differences from the ck control.
TABLE 2 growth promoting ability of Bacillus FJAT-10275 on tomato seeds
Note: in the above table, ab represents significant difference (P <0.05)
In conclusion, Bacillus FJAT-10275 has the functions of phosphate dissolving and growth promoting. In the practical application process, the fermentation liquor can be diluted by at least 1000 times to prepare the productTo a concentration of 1 × 105-2×105The bacterial suspension of cfu/m L is soaked in the solution for 2-3 days, and then the solution is placed at 25-30 ℃ and is irradiated for 16-20 h/day, or the bacterial suspension is irrigated into the soil to promote the growth of plants.
It should be noted that, although the above embodiments have been described herein, the invention is not limited thereto. Therefore, based on the innovative concepts of the present invention, the technical solutions of the present invention can be directly or indirectly applied to other related technical fields by making changes and modifications to the embodiments described herein, or by using equivalent structures or equivalent processes performed in the content of the present specification and the attached drawings, which are included in the scope of the present invention.
Sequence listing
<110> institute of agricultural biological resources of academy of agricultural sciences of Fujian province
<120> Bacillus phosphate solubilizing bacteria and application thereof
<130>10275
<160>1
<170>SIPOSequenceListing 1.0
<210>1
<211>1422
<212>DNA
<213> Bacillus subtilis subsp. inaquosum
<400>1
tgcagtcgag cggacagatg ggagcttgct ccctgatgtt agcggcggac gggtgagtaa 60
cacgtgggta acctgcctgt aagactggga taactccggg aaaccggggc taataccgga 120
tggttgtttg aaccgcatgg ttcaaacata aaaggtggct tcggctacca cttacagatg 180
gacccgcggc gcattagcta gttggtgagg taacggctca ccaaggcaac gatgcgtagc 240
cgacctgaga gggtgatcgg ccacactggg actgagacac ggcccagact cctacgggag 300
gcagcagtag ggaatcttcc gcaatggacg aaagtctgac ggagcaacgc cgcgtgagtg 360
atgaaggttt tcggatcgta aagctctgtt gttagggaag aacaagtacc gttcgaatag 420
ggcggtacct tgacggtacc taaccagaaa gccacggcta actacgtgcc agcagccgcg 480
gtaatacgta ggtggcaagc gttgtccgga attattgggc gtaaagggct cgcaggcggt 540
ttcttaagtc tgatgtgaaa gcccccggct caaccgggga gggtcattgg aaactgggga 600
acttgagtgc agaagaggag agtggaattc cacgtgtagc ggtgaaatgc gtagagatgt 660
ggaggaacac cagtggcgaa ggcgactctc tggtctgtaa ctgacgctga ggagcgaaag 720
cgtggggagc gaacaggatt agataccctg gtagtccacg ccgtaaacga tgagtgctaa 780
gtgttagggg gtttccgccc cttagtgctg cagctaacgc attaagcact ccgcctgggg 840
agtacggtcg caagactgaa actcaaagga attgacgggg gcccgcacaa gcggtggagc 900
atgtggttta attcgaagca acgcgaagaa ccttaccagg tcttgacatc ctctgacaat 960
cctagagata ggacgtcccc ttcgggggca gagtgacagg tggtgcatgg ttgtcgtcag 1020
ctcgtgtcgt gagatgttgg gttaagtccc gcaacgagcg caacccttga tcttagttgc 1080
cagcattcag ttgggcactc taaggtgact gccggtgaca aaccggagga aggtggggat 1140
gacgtcaaat catcatgccc cttatgacct gggctacaca cgtgctacaa tggacagaac 1200
aaagggcagc gaaaccgcga ggttaagcca atcccacaaa tctgttctca gttcggatcg 1260
cagtctgcaa ctcgactgcg tgaagctgga atcgctagta atcgcggatc agcatgccgc 1320
ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accacgagag tttgtaacac 1380
ccgaagtcgg tgaggtaacc ttttaggagc cagccgccga ag 1422
Claims (5)
1. A bacillus phosphate solubilizing bacterium, comprising: the Bacillus is Bacillus subtilis FJAT-10275 with the scientific name of Bacillus subtilis subsp. inaquosum FJAT-10275, which is preserved in the common microorganism center of China Committee for culture Collection of microorganisms with the preservation number of CGMCC NO.16327, the preservation date of 2018, 8 and 21 days and the preservation address of the institute of microbiology, China academy of sciences, Beijing, China.
2. Use of the bacillus phosphate solubilizing bacterium of claim 1 for degrading a poorly soluble phosphate.
3. Use of the bacillus phosphate solubilizing bacterium of claim 1 for promoting germination of a plant seed.
4. The use of Bacillus phosphate solubilizing bacteria for promoting germination of plant seeds as claimed in claim 3, wherein said Bacillus fermentation broth is formulated to have a concentration of 1 × 105-2×105cfu/m L, soaking the strain for 2-3 days, and placing at 25-30 deg.C under illumination for 16-20 h/day.
5. A plant growth promoting microbial inoculum is characterized in that: the microbial inoculum comprises the bacillus of claim 1.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112592855A (en) * | 2020-12-17 | 2021-04-02 | 有研资源环境技术研究院(北京)有限公司 | Bacillus subtilis and method for treating uranium and cadmium polluted water body by using same |
| CN112625947A (en) * | 2020-12-16 | 2021-04-09 | 江苏省中国科学院植物研究所 | Bacillus subtilis capable of dissolving phosphorus strongly, carbon-based microbial compound fertilizer thereof and application of bacillus subtilis |
| CN113215061A (en) * | 2021-06-15 | 2021-08-06 | 四川农业大学 | Bacillus subtilis SCAU-Z8 and application thereof |
| CN113249266A (en) * | 2021-06-22 | 2021-08-13 | 云南农业大学 | Bacillus subtilis capable of efficiently dissolving phosphorus and promoting sugarcane germination and application thereof |
| CN114686398A (en) * | 2022-03-17 | 2022-07-01 | 山东省林业科学研究院 | Biological water-retaining material and preparation method and application thereof |
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| CN112625947A (en) * | 2020-12-16 | 2021-04-09 | 江苏省中国科学院植物研究所 | Bacillus subtilis capable of dissolving phosphorus strongly, carbon-based microbial compound fertilizer thereof and application of bacillus subtilis |
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| CN112592855A (en) * | 2020-12-17 | 2021-04-02 | 有研资源环境技术研究院(北京)有限公司 | Bacillus subtilis and method for treating uranium and cadmium polluted water body by using same |
| CN113215061A (en) * | 2021-06-15 | 2021-08-06 | 四川农业大学 | Bacillus subtilis SCAU-Z8 and application thereof |
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| CN113249266B (en) * | 2021-06-22 | 2023-02-03 | 云南农业大学 | Bacillus subtilis capable of efficiently dissolving phosphorus and promoting sugarcane germination and application thereof |
| CN114686398A (en) * | 2022-03-17 | 2022-07-01 | 山东省林业科学研究院 | Biological water-retaining material and preparation method and application thereof |
| CN114686398B (en) * | 2022-03-17 | 2022-10-11 | 山东省林业科学研究院 | Biological water-retaining material and preparation method and application thereof |
| WO2023173735A1 (en) * | 2022-03-17 | 2023-09-21 | 山东省林业科学研究院 | Biological water-retaining material, and preparation method therefor and use thereof |
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