CN1814040A - Compound liquid capsule of Chinese caterpillar fungus, preparing method and quality control method - Google Patents
Compound liquid capsule of Chinese caterpillar fungus, preparing method and quality control method Download PDFInfo
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- CN1814040A CN1814040A CNA2005100618452A CN200510061845A CN1814040A CN 1814040 A CN1814040 A CN 1814040A CN A2005100618452 A CNA2005100618452 A CN A2005100618452A CN 200510061845 A CN200510061845 A CN 200510061845A CN 1814040 A CN1814040 A CN 1814040A
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Abstract
The present invention discloses a compound cordyceps liquid capsule. Its contents are formed from 20-30 weight portions of compound cordyceps extract powder, 28-40 weight portions of diluting agent and 1-1.8 weight portions of suspension adjuvant. The described compound cordyceps extract powder is made up by using 6 Chinese medicinal materials of cordyceps, epimedium, lyceum berry, crataegus fruit, nardostachys root and royal jelly through the processes of extraction and drying.
Description
Technical field
The present invention relates to the field of Chinese medicines, especially a kind of compound liquid capsule of Chinese caterpillar fungus and preparation method thereof and method of quality control.
Background technology
Listing kind " compound Chinese caterpillar fungus oral liquid " is mainly made by Cordyceps, Herba Epimedii, Fructus Lycii, Lac regis apis, Fructus Crataegi, Radix Et Rhizoma Nardostachyos, and function cures mainly and is nourishing the liver and kidney, tonifying the lung mind calming, the dissipating depression of QI of being amusing.Be used for the prolonged illness weakness, hypomnesis, diseases such as cough with asthma, sexual impotence, hyperlipidemia.Be widely used in clinically, comparatively ideal effect has been arranged.But because the unstability of oral liquid, give this product storage, the aspect such as transport, take and make troubles, also influenced the effect of product.
Summary of the invention
Purpose of the present invention is exactly to solve the problem that exists on the prior art, and the compound liquid capsule of Chinese caterpillar fungus and preparation method thereof and the method for quality control of a kind of safe in utilization, steady quality, evident in efficacy, taking convenience is provided.
Purpose of the present invention can take following method to realize:
A kind of compound liquid capsule of Chinese caterpillar fungus is characterized in that its content is made by following raw material: compound Chinese caterpillar fungus extract powder 20～30 weight portions, diluent 28～40 weight portions, suspending agent 1～1.8 weight portion;
Described compound Chinese caterpillar fungus extract powder is made through extraction, drying by following raw material of Chinese medicine proportioning: Cordyceps 10 weight portions, Herba Epimedii 100 weight portions, Fructus Lycii 125 weight portions, Fructus Crataegi 50 weight portions, Radix Et Rhizoma Nardostachyos 5 weight portions, Lac regis apis 5 weight portions.
Described diluent is any or the combination more than two kinds in Oleum Arachidis hypogaeae semen, soybean oil, Oleum Brassicae campestris, the Semen Maydis oil, or Polyethylene Glycol, propylene glycol.
Described suspending agent is one or more the mixture in Cera Flava, lecithin, aluminum monostearate, the ethyl cellulose.
The preferred version content is made by following raw material: compound Chinese caterpillar fungus extract powder 22 weight portions, diluent 36.6 weight portions, suspending agent 1.4 weight portions.
A kind of preparation method of compound liquid capsule of Chinese caterpillar fungus is characterized in that comprising the steps:
Get Cordyceps by certain weight ratio and add 10 times of water gagings decoctions 1 hour, filter, filtrate device is in addition preserved; Medicinal residues add Herba Epimedii, Fructus Lycii, Fructus Crataegi, the Radix Et Rhizoma Nardostachyos of certain weight ratio, add 10 times of water gagings and decoct secondary, each 1 hour, collecting decoction filtered, filtrate is concentrated into 1500ml, add equivalent ethanol, stir evenly, left standstill 24 hours, get supernatant, decompression recycling ethanol adds above-mentioned Cordyceps decocting liquid, and being evaporated to relative density is the clear paste of 1.10-1.15 (70-80 ℃), spray drying, 180 ± 5 ℃ of inlet temperature, 70 ± 5 ℃ of leaving air temps get dry powder, add the Lac regis apis of certain weight ratio, be the compound Chinese caterpillar fungus extract powder;
Get diluent, suspending agent, be heated to 60 ℃～70 ℃, stir and make dissolving, mixing, 60 ℃～70 ℃ insulations; Other gets the compound Chinese caterpillar fungus extract powder and adds in the above-mentioned solution, crosses 100 mesh sieves behind the mix homogeneously, drops into liquid capsule and produces in the filling machine and make capsule, promptly.
The content of compound liquid capsule of Chinese caterpillar fungus is made up of compound Chinese caterpillar fungus extract powder and diluent, suspending agent.Suspending agent can be one or more the mixture in Cera Flava, lecithin, aluminum monostearate, the ethyl cellulose etc., be preferably Cera Flava, and diluent can be one or more the mixture in soybean oil, Oleum Arachidis hypogaeae semen, Oleum Brassicae campestris, Oleum Helianthi, Semen Maydis oil, rapeseed oil and other edible plants oil, and Polyethylene Glycol, propylene glycol etc.In view of big, the stay in grade of cheap, output of soybean oil, so be decided to be optimum selection.
Filter out diluent, the suspending agent of compound liquid capsule of Chinese caterpillar fungus content by experiment.(T=20 ± 2 ℃, RH=40～80%) successive stability test carries out at ambient temperature to adopt room temperature reserved sample observing method:
1, gets compound Chinese caterpillar fungus extract powder 2200g, slowly add in the Oleum Arachidis hypogaeae semen of 3800g, constantly stir, cross 100 mesh sieves behind the mix homogeneously, drop in the liquid capsule production filling machine and make capsule, promptly.The method content material fluidity is better, and pouring process is more easily realized, but finished product stability is relatively poor, promptly occurs stratified phenomenon after the fill, can not satisfy the requirement of pharmacopeia to liquid capsule preparation stability.
2, get the soybean oil of 3730g, be heated to 60 ℃～70 ℃ insulations, get Cera Flava 70g and cut into pieces, add in the vegetable oil, insulated and stirred makes dissolving.Other gets in the compound Chinese caterpillar fungus extract powder 2200g adding vegetable oil solution, crosses 100 mesh sieves behind the mix homogeneously, drops into liquid capsule production fill and becomes capsule, promptly.The method content material fluidity is better, pouring process realizes that more easily finished product stability improves a lot, but the partial content thing has lamination in 1 week, tangible lamination all appears in most of liquid capsule after January, can not satisfy the requirement of pharmacopeia to liquid capsule preparation stability.
3, get the soybean oil of 3660g, be heated to 60 ℃～70 ℃ insulations, get Cera Flava 140g and cut into pieces, add in the vegetable oil, insulated and stirred makes dissolving.Other gets in the compound Chinese caterpillar fungus extract powder 2200g adding vegetable oil solution, crosses 100 mesh sieves behind the mix homogeneously, drops into liquid capsule production filling machine and makes capsule, promptly.The method content Flow of Goods and Materials sexual satisfaction fill requirement, finished product stability is good, through the room temperature reserved sample observing, the content lamination do not occur, and satisfies the requirement of pharmacopeia to liquid capsule preparation stability in 1 year half.
4, get the soybean oil of 3600g, be heated to 60 ℃～70 ℃ insulations, get Cera Flava 200g and cut into pieces, add in the vegetable oil, insulated and stirred makes dissolving.Other gets in the compound Chinese caterpillar fungus extract powder 2200g adding vegetable oil solution, crosses 100 mesh sieves behind the mix homogeneously, drops into liquid capsule production filling machine and makes capsule.The method content material fluidity is relatively poor, thickness very at room temperature, and obstruction in various degree appears in the liquid capsule filling machine, can't finish fill smoothly, can not satisfy the needs of big production.
5, taking polyethylene glycol 3500g and propylene glycol 300g mix homogeneously are heated to 60 ℃～70 ℃ insulations, and other gets compound Chinese caterpillar fungus extract powder 2200g adding, cross 100 mesh sieves behind the mix homogeneously, drop in the liquid capsule production filling machine and make soft gelatin capsule.The method content material fluidity is better, pouring process is more easily realized, through the room temperature reserved sample observing, the content lamination appearred after 1 year, do not satisfy the requirement of pharmacopeia yet, do diluent with Polyethylene Glycol in addition, because Polyethylene Glycol is difficult for removing liquid capsule preparation stability, behavior has very big interference to thin layer when thin-layer qualitative is analyzed, wayward end product quality.
According to above a large amount of experimentation, thereby weight proportion raw material, optimum weight proportioning raw material and the preparation method of compound liquid capsule of Chinese caterpillar fungus content of the present invention have been determined.
A kind of method of quality control of compound liquid capsule of Chinese caterpillar fungus is characterized in that comprising qualitative identification and assay two parts:
The qualitative identification part:
A, get this product content 6g, add kieselguhr 4g, mix thoroughly, add the methanol supersound process, filter, filtrate water bath method, residue add water makes dissolving, with ether extraction three times, merges ether solution, and evaporate to dryness, residue add methanol 1ml makes dissolving, as need testing solution.Other gets the ursolic acid reference substance, adds methanol and makes the solution that every 1ml contains 1mg, in contrast product solution.Test according to thin layer chromatography, draw each 5 μ l of above-mentioned two kinds of solution, put respectively in same be on the silica gel g thin-layer plate of adhesive with the sodium carboxymethyl cellulose, chloroform-acetone with 8: 2 ratios is developing solvent, launch, take out, dry, spray is with 10% ethanol solution of sulfuric acid, and it is clear to be heated to the speckle colour developing at 105 ℃.In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the speckle of same color; Put under the 365nm ultra-violet lamp and inspect, show the fluorescence speckle of same color;
B. get the water liquid behind the ether extraction under a item, extract three times with the ethyl acetate jolting, each 20ml, combined ethyl acetate liquid, water bath method, residue add methanol 1ml makes dissolving, as need testing solution; Other gets Fructus Lycii control medicinal material 1g, adds water 50ml, decocts 20 minutes, filters, and filtrate being concentrated into is about 20ml, with ether extraction three times, merges ether solution, and evaporate to dryness, residue add methanol 1ml makes dissolving, makes control medicinal material solution; According to thin layer chromatography test, draw each 5 μ l of above-mentioned two kinds of solution, put respectively in same be on the silica gel g thin-layer plate of adhesive with the sodium carboxymethyl cellulose, cyclohexane extraction-ethyl acetate-methanol with 6: 4: 1 ratios is developing solvent, launches, and takes out, dry, put under the 365nm ultra-violet lamp and inspect; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the fluorescence speckle of same color;
The assay part:
Chromatographic condition and system suitability test are filler with the octadecylsilane chemically bonded silica; Acetonitrile-water with 27: 73 ratios is a mobile phase; The detection wavelength is 270nm.Number of theoretical plate calculates by the icariin peak should be not less than 1500.
In order to control product quality better, the present invention also provides method of quality control, comprises qualitative identification and assay two parts:
The qualitative identification part:
A, get this product content 6g, add kieselguhr 4g, mix thoroughly, add the methanol supersound process, filter, filtrate water bath method, residue add water makes dissolving, with ether extraction three times, merges ether solution, and evaporate to dryness, residue add methanol 1ml makes dissolving, as need testing solution.Other gets the ursolic acid reference substance, adds methanol and makes the solution that every 1ml contains 1mg, in contrast product solution.Test according to thin layer chromatography, draw each 5 μ l of above-mentioned two kinds of solution, put respectively in same be on the silica gel g thin-layer plate of adhesive with the sodium carboxymethyl cellulose, chloroform-acetone with 8: 2 ratios is developing solvent, launch, take out, dry, spray is with 10% ethanol solution of sulfuric acid, and it is clear to be heated to the speckle colour developing at 105 ℃.In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the speckle of same color; Put under the 365nm ultra-violet lamp and inspect, show the fluorescence speckle of same color.
B. get the water liquid behind the ether extraction under a item, extract three times with the ethyl acetate jolting, each 20ml, combined ethyl acetate liquid, water bath method, residue add methanol 1ml makes dissolving, as need testing solution; Other gets Fructus Lycii control medicinal material 1g, adds water 50ml, decocts 20 minutes, filters, and filtrate being concentrated into is about 20ml, with ether extraction three times, merges ether solution, and evaporate to dryness, residue add methanol 1ml makes dissolving, makes control medicinal material solution; According to thin layer chromatography test, draw each 5 μ l of above-mentioned two kinds of solution, put respectively in same be on the silica gel g thin-layer plate of adhesive with the sodium carboxymethyl cellulose, cyclohexane extraction-ethyl acetate-methanol with 6: 4: 1 ratios is developing solvent, launches, and takes out, dry, put under the 365nm ultra-violet lamp and inspect; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the fluorescence speckle of same color.
The assay part:
Chromatographic condition and system suitability test are filler with the octadecylsilane chemically bonded silica; Acetonitrile-water with 27: 73 ratios is a mobile phase; The detection wavelength is 270nm.Number of theoretical plate calculates by the icariin peak should be not less than 1500.
It is an amount of that the preparation precision of reference substance solution takes by weighing the icariin reference substance, adds the mobile phase dissolving and make the reference substance solution that every 1ml contains 30 μ g, promptly.
This product content is got in the preparation of need testing solution, grinds well, and takes by weighing 2g, the accurate title, decide, and adds kieselguhr 2g, mixes thoroughly, put in the apparatus,Soxhlet's, it is an amount of to add petroleum ether, and reflux 1 hour discards petroleum ether liquid, medicinal residues are flung to petroleum ether, continuation was extracted 2 hours with the methanol Soxhlet, and methanol solution water bath method, residue add the Diluted Alcohol dissolving and quantitatively be transferred in the 100ml measuring bottle, add Diluted Alcohol to scale, shake up, precision is measured 5ml, puts in the 10ml measuring bottle, add Diluted Alcohol to scale, shake up, with the microporous filter membrane filtration of 0.45 μ m, promptly.
Algoscopy: accurate respectively reference substance solution and each 10 μ l of need testing solution of drawing, inject chromatograph of liquid, measure, promptly.
Use above technical scheme, in conjunction with existing Chinese Pharmacopoeia correlation technique and regulation, to making the stable quality investigation that product has carried out 18 months, the result evenly reaches standard, so technical solution of the present invention is feasible.
Compound liquid capsule of Chinese caterpillar fungus of the present invention belongs to a kind of of hard capsule, just content is a liquid, it has saved encapsulated preceding many steps, the equipment needed thereby small investment, compare with former dosage form oral liquid, it has storage, carries, the advantage of taking convenience, simultaneously more stable quality, because liquid capsule does not contain sugar, can be fit to more patient and take again.
The specific embodiment
Embodiment 1:
Compound Chinese caterpillar fungus extract powder 3kg, soybean oil 4kg, Cera Flava 0.18kg.
Its preparation method: get soybean oil, be heated to 60 ℃～70 ℃ insulations, get Cera Flava and cut into pieces, add in the soybean oil, stir and make dissolving, get extract powder and add in the soybean oil solution, grind well, make medicinal liquid; Use the liquid capsule filling machine, make 10000 of liquid capsules.
Embodiment 2:
Compound Chinese caterpillar fungus extract powder 2kg, Oleum Arachidis hypogaeae semen 2.8kg, propylene glycol 0.1kg, Cera Flava 0.1kg
Its preparation method is identical with embodiment 1.
Embodiment 3:
Compound Chinese caterpillar fungus extract powder 2.4kg, Oleum Brassicae campestris 3.5kg, Cera Flava 0.15kg.
Its preparation method is identical with embodiment 1.
Embodiment 4: embodiment 1 is made product carry out quality inspection
Qualitative identification
(1) gets this product content 6g, add kieselguhr 4g, mix thoroughly, add methanol 30ml, supersound process 30 minutes, filter, filtrate water bath method, residue add water 20ml makes dissolving, with ether extraction three times, and each 20ml, merge ether solution, evaporate to dryness, residue add methanol 1ml makes dissolving, as need testing solution.Other gets the ursolic acid reference substance, adds methanol and makes the solution that every 1ml contains 1mg, in contrast product solution.Test according to thin layer chromatography, draw each 5 μ l of above-mentioned two kinds of solution, put respectively in same be on the silica gel g thin-layer plate of adhesive with the sodium carboxymethyl cellulose, with chloroform-acetone (8: 2) is developing solvent, launch, take out, dry, spray is with 10% ethanol solution of sulfuric acid, and it is clear to be heated to the speckle colour developing at 105 ℃.In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the speckle of same color; Put under the ultra-violet lamp (365nm) and inspect, show the fluorescence speckle of same color.
(2) get the water liquid behind the ether extraction under the item of [discriminating] (1), extract three times with the ethyl acetate jolting, each 20ml, combined ethyl acetate liquid, water bath method, residue add methanol 1ml makes dissolving, as need testing solution.Other gets Fructus Lycii control medicinal material 1g, adds water 50ml, decocts 20 minutes, filters, and filtrate being concentrated into is about 20ml, with ether extraction three times, merges ether solution, and evaporate to dryness, residue add methanol 1ml makes dissolving, makes control medicinal material solution.According to thin layer chromatography test, draw each 5 μ l of above-mentioned two kinds of solution, put respectively in same be on the silica gel g thin-layer plate of adhesive with the sodium carboxymethyl cellulose, with cyclohexane extraction-ethyl acetate-methanol (6: 4: 1) is developing solvent, launches, and takes out, dry, put under the ultra-violet lamp (365nm) and inspect.In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the fluorescence speckle of same color.
[assay] is according to high effective liquid chromatography for measuring.
Chromatographic condition and system suitability test are filler with the octadecylsilane chemically bonded silica; With acetonitrile-water (27: 73) is mobile phase; The detection wavelength is 270nm.Number of theoretical plate calculates by the icariin peak should be not less than 1500.
It is an amount of that the preparation precision of reference substance solution takes by weighing the icariin reference substance, adds the mobile phase dissolving and make the reference substance solution that every 1ml contains 30 μ g, promptly.
This product content under the content uniformity item is got in the preparation of need testing solution, grinds well, and takes by weighing 2g, the accurate title, decide, and adds kieselguhr 2g, mixes thoroughly, put in the apparatus,Soxhlet's, it is an amount of to add petroleum ether, and reflux 1 hour discards petroleum ether liquid, medicinal residues are flung to petroleum ether, continuation was extracted 2 hours with the methanol Soxhlet, and methanol solution water bath method, residue add the Diluted Alcohol dissolving and quantitatively be transferred in the 100ml measuring bottle, add Diluted Alcohol to scale, shake up, precision is measured 5ml, puts in the 10ml measuring bottle, add Diluted Alcohol to scale, shake up, filter with microporous filter membrane (0.45 μ m), promptly.
Accurate respectively reference substance solution and each the 10 μ l of need testing solution of drawing of algoscopy inject chromatograph of liquid, measure, promptly.
Every of this product contains Herba Epimedii with icariin (C
33H
40O
15) meter, must not be less than 1.0mg.
Claims (6)
1, a kind of compound liquid capsule of Chinese caterpillar fungus is characterized in that its content is made by following raw material: compound Chinese caterpillar fungus extract powder 20～30 weight portions, diluent 28～40 weight portions, Cera Flava 1～1.8 weight portion;
Described compound Chinese caterpillar fungus extract powder is made through extraction, drying by following raw material of Chinese medicine proportioning: Cordyceps 10 weight portions, Herba Epimedii 100 weight portions, Fructus Lycii 125 weight portions, Fructus Crataegi 50 weight portions, Radix Et Rhizoma Nardostachyos 5 weight portions, Lac regis apis 5 weight portions.
2, compound liquid capsule of Chinese caterpillar fungus according to claim 1 is characterized in that: described diluent is any or the combination more than two kinds in Oleum Arachidis hypogaeae semen, soybean oil, Oleum Brassicae campestris, the Semen Maydis oil, or Polyethylene Glycol, propylene glycol.
3. described suspending agent is one or more the mixture in Cera Flava, lecithin, aluminum monostearate, the ethyl cellulose.
4, compound liquid capsule of Chinese caterpillar fungus according to claim 1 is characterized in that its content the best is made by following raw material: compound Chinese caterpillar fungus extract powder 22 weight portions, diluent 36.6 weight portions, Cera Flava 1.4 weight portions.
5, the preparation method of compound liquid capsule of Chinese caterpillar fungus according to claim 1 is characterized in that comprising the steps:
Get Cordyceps by certain weight ratio and add 10 times of water gagings decoctions 1 hour, filter, filtrate device is in addition preserved; Medicinal residues add Herba Epimedii, Fructus Lycii, Fructus Crataegi, the Radix Et Rhizoma Nardostachyos of certain weight ratio, add 10 times of water gagings and decoct secondary, each 1 hour, collecting decoction filtered, filtrate is concentrated into 1500ml, add equivalent ethanol, stir evenly, left standstill 24 hours, get supernatant, decompression recycling ethanol adds above-mentioned Cordyceps decocting liquid, and being evaporated to relative density is the clear paste of 1.10-1.15 (70-80 ℃), spray drying, 180 ± 5 ℃ of inlet temperature, 70 ± 5 ℃ of leaving air temps get dry powder, add the Lac regis apis of certain weight ratio, be the compound Chinese caterpillar fungus extract powder;
Get diluent, suspending agent, be heated to 60 ℃～70 ℃, stir and make dissolving, mixing, 60 ℃～70 ℃ insulations; Other gets the compound Chinese caterpillar fungus extract powder and adds in the above-mentioned solution, crosses 100 mesh sieves behind the mix homogeneously, drops into liquid capsule and produces in the filling machine and make capsule, promptly.
6, the method for quality control of compound liquid capsule of Chinese caterpillar fungus according to claim 1 is characterized in that comprising qualitative identification and assay two parts:
The qualitative identification part:
A, get this product content 6g, add kieselguhr 4g, mix thoroughly, add the methanol supersound process, filter, filtrate water bath method, residue add water makes dissolving, with ether extraction three times, merges ether solution, and evaporate to dryness, residue add methanol 1ml makes dissolving, as need testing solution.Other gets the ursolic acid reference substance, adds methanol and makes the solution that every 1ml contains 1mg, in contrast product solution.Test according to thin layer chromatography, draw each 5 μ l of above-mentioned two kinds of solution, put respectively in same be on the silica gel g thin-layer plate of adhesive with the sodium carboxymethyl cellulose, chloroform-acetone with 8: 2 ratios is developing solvent, launch, take out, dry, spray is with 10% ethanol solution of sulfuric acid, and it is clear to be heated to the speckle colour developing at 105 ℃.In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the speckle of same color; Put under the 365nm ultra-violet lamp and inspect, show the fluorescence speckle of same color;
B. get the water liquid behind the ether extraction under a item, extract three times with the ethyl acetate jolting, each 20ml, combined ethyl acetate liquid, water bath method, residue add methanol 1ml makes dissolving, as need testing solution; Other gets Fructus Lycii control medicinal material 1g, adds water 50ml, decocts 20 minutes, filters, and filtrate being concentrated into is about 20ml, with ether extraction three times, merges ether solution, and evaporate to dryness, residue add methanol 1ml makes dissolving, makes control medicinal material solution; According to thin layer chromatography test, draw each 5 μ l of above-mentioned two kinds of solution, put respectively in same be on the silica gel g thin-layer plate of adhesive with the sodium carboxymethyl cellulose, cyclohexane extraction-ethyl acetate-methanol with 6: 4: 1 ratios is developing solvent, launches, and takes out, dry, put under the 365nm ultra-violet lamp and inspect; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the fluorescence speckle of same color;
The assay part:
Chromatographic condition and system suitability test are filler with the octadecylsilane chemically bonded silica; Acetonitrile-water with 27: 73 ratios is a mobile phase; The detection wavelength is 270nm.Number of theoretical plate calculates by the icariin peak should be not less than 1500.
It is an amount of that the preparation precision of reference substance solution takes by weighing the icariin reference substance, adds the mobile phase dissolving and make the reference substance solution that every 1ml contains 30 μ g, promptly.
This product content is got in the preparation of need testing solution, grinds well, and takes by weighing 2g, the accurate title, decide, and adds kieselguhr 2g, mixes thoroughly, put in the apparatus,Soxhlet's, it is an amount of to add petroleum ether, and reflux 1 hour discards petroleum ether liquid, medicinal residues are flung to petroleum ether, continuation was extracted 2 hours with the methanol Soxhlet, and methanol solution water bath method, residue add the Diluted Alcohol dissolving and quantitatively be transferred in the 100ml measuring bottle, add Diluted Alcohol to scale, shake up, precision is measured 5ml, puts in the 10ml measuring bottle, add Diluted Alcohol to scale, shake up, with the microporous filter membrane filtration of 0.45 μ m, promptly.
Algoscopy: accurate respectively reference substance solution and each 10 μ l of need testing solution of drawing, inject chromatograph of liquid, measure, promptly.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101797302A (en) * | 2010-04-07 | 2010-08-11 | 宁夏启元国药有限公司 | Method for identifying medlar and common cnidium fruit in Qirong tablets |
| CN104585742A (en) * | 2013-10-31 | 2015-05-06 | 江苏学府生物工程有限公司 | A compound cordyceps sinensis oil soft capsule production method |
| CN104597195A (en) * | 2013-10-30 | 2015-05-06 | 宜昌山城水都冬虫夏草有限公司 | Method for discriminating cordyceps sinensis storage time |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102626425B (en) * | 2012-05-09 | 2013-12-11 | 马建中 | Cordyceps sinensis powder soft capsule and preparation method thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100375617C (en) * | 2003-07-22 | 2008-03-19 | 范敏华 | Liquid capsule and production method thereof |
| CN1605354A (en) * | 2003-10-10 | 2005-04-13 | 杜癸泉 | Yang strengthening capsule and its preparation |
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2005
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101797302A (en) * | 2010-04-07 | 2010-08-11 | 宁夏启元国药有限公司 | Method for identifying medlar and common cnidium fruit in Qirong tablets |
| CN101797302B (en) * | 2010-04-07 | 2012-03-07 | 宁夏启元国药有限公司 | Method for identifying medlar and common cnidium fruit in Qirong tablets |
| CN104597195A (en) * | 2013-10-30 | 2015-05-06 | 宜昌山城水都冬虫夏草有限公司 | Method for discriminating cordyceps sinensis storage time |
| CN104597195B (en) * | 2013-10-30 | 2017-03-15 | 宜昌山城水都冬虫夏草有限公司 | A kind of method for differentiating Cordyceps storage time |
| CN104585742A (en) * | 2013-10-31 | 2015-05-06 | 江苏学府生物工程有限公司 | A compound cordyceps sinensis oil soft capsule production method |
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| CN1814040B (en) | 2011-03-30 |
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