CN1861180A - Method for preparing extractive of traditional Chinese medicine sarsa and its quality control method - Google Patents
Method for preparing extractive of traditional Chinese medicine sarsa and its quality control method Download PDFInfo
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- CN1861180A CN1861180A CN 200510200269 CN200510200269A CN1861180A CN 1861180 A CN1861180 A CN 1861180A CN 200510200269 CN200510200269 CN 200510200269 CN 200510200269 A CN200510200269 A CN 200510200269A CN 1861180 A CN1861180 A CN 1861180A
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Abstract
A process for extracting the active components from China root greenbrier rhizome includes such steps as decocting in water, depositing in alcohol, and refining by macroreticular resin column. Its quality control method is also disclosed.
Description
Technical field
The present invention relates to a kind of preparation method of Chinese medicine extract, especially a kind of preparation method of Chinese medicine Rhizoma Smilacis Chinensis extract also relates to the method for quality control of this Rhizoma Smilacis Chinensis extract simultaneously, belongs to the field of Chinese medicines.
Background technology
Rhizoma Smilacis Chinensis is a liliaceous plant, contains multiple smilacin in this plant roots and stems.Show that after deliberation pulling out the chinaroot greenbrier total saponins has heat-clearing and toxic substances removing, physiologically actives such as dispersing swelling and dissipating binds are used for gynecological's adnexitis and inflammatory masses of ovarian appendages, and better curative effect is arranged.To be paid attention to by increasing people for the method for from the Rhizoma Smilacis Chinensis medical material, extracting total saponins and the pharmaceutical usage that pulls out the chinaroot greenbrier total saponins.The extractive technique of the Rhizoma Smilacis Chinensis total saponins of present stage exists purification techniques and falls behind, and saponin content is lower, the more deficiency that waits of impurity.
Summary of the invention
Extract process for purification at present Rhizoma Smilacis Chinensis and fall behind, the problem that Rhizoma Smilacis Chinensis total saponins content is lower the invention provides a kind of new extraction process for purification in advance to solve.At first be the extraction part in this method, can extract by two kinds of methods:
Method one: get Rhizoma Smilacis Chinensis, decoct with water secondary, each 2 hours, add for the first time 8 times of amounts of water, add 6 times of amounts of water for the second time, merge decocting liquid, filter, filtrate decompression concentrate (70～80 ℃ ,-0.08Mpa) to relative density 1.20～1.25 (80 ℃), room temperature to be chilled to adds ethanol and makes and contain amount of alcohol and reach 40%, leaves standstill cold preservation 24 hours, filter, decompression filtrate recycling ethanol (70～80 ℃ ,-0.08Mpa), and continuing to be concentrated into relative density 1.20～1.25 (80 ℃), spray drying obtains medicated powder.
Method two: get the Rhizoma Smilacis Chinensis medical material, add 6～8 times of 40%～70% alcohol reflux 2～3 times, each 2～3 hours, merge ethanol liquid, filter, decompression filtrate recycling ethanol, and continue to be concentrated into relative density 1.20～1.25, spray drying obtains medicated powder.
Through extracting resulting medicated powder, can contain 20%～30% Rhizoma Smilacis Chinensis total saponins, in order to obtain the higher extract of purity, also need it is made with extra care, method is as follows:
Get the medicated powder that obtains after above spray is done, after being dissolved in water, use n-butanol extraction, merge n-butyl alcohol liquid, go up macroporous resin column absorption after the thick paste behind the recovery n-butyl alcohol is dissolved in water, impurity is removed in washing, use the alcoholic solution eluting, collect ethanol liquid, reclaim ethanol to relative density 1.20～1.25 (80 ℃), spray drying is Rhizoma Smilacis Chinensis total saponins extract.
Above-mentioned big pore resin should be the polystyrene type macroporous adsorbent resin; Spray-dired condition is 190 ± 5 ℃ of inlet temperature, 100 ± 5 ℃ of leaving air temps.
Through after the above extraction refinement treatment, the content of extract obtained middle Rhizoma Smilacis Chinensis total saponins can reach more than 80%.The inventor has carried out assay by following steps to the extract by the inventive method gained: will extract gained medicated powder respectively and spray drying gained medicated powder adds the methanol supersound process, 5% vanillin-glacial acetic acid solution 0.2ml perchloric acid the 0.8ml that adds new preparation, jump a queue, shake up, in 70 ℃ of heating in water bath 20 minutes, take out, precision adds ethyl acetate 5ml after being cooled to room temperature, shaking up, is blank with the same solvent, measures trap in the 560nm place, the result is in diosgenin, extract and refining after pull out the chinaroot greenbrier total saponin content respectively greater than 20% and 80%, reached purified purpose, also reached goal of the invention.
Pull out the chinaroot greenbrier extract by what above method obtained, can be advantageously used in the preparation of multiple dosage form, as the spray powder filling being become capsule or spray powder being added an amount of soybean oil, be pressed into soft capsule after grinding evenly with colloid mill or add an amount of little smart fiber and the starch compacting in flakes, also can make injection after the extract dissolving.
At last, in order to control the quality of extract of the present invention, the inventor repays principal and has set up the method for quality control that pulls out chinaroot greenbrier total saponins and preparation thereof, and this method comprises assay and qualitative identification two parts.
Content assaying method:
Chromatographic condition and system suitability test are filler with octadecylsilane chemically bonded silica; 70～90: 30～10 acetonitrile-water is a mobile phase; Flow velocity 0.8～1.2ml/min, 25～40 ℃ of column temperatures, ultraviolet or evaporative light scattering detector detect.
It is an amount of that the preparation precision of reference substance solution takes by weighing the diosgenin reference substance, adds dissolve with methanol, makes and determine concentration solution, product solution I in contrast; Get reference substance solution I, add 1～2 times of methanol dilution, make reference substance solution II, promptly.
It is fixed that the accurate title of this product is got in the preparation of need testing solution, added methanol extraction 20～30 minutes, puts cold, filter,, filter with an amount of methanol wash residue, merge methanol solution, water bath method, residue add water makes dissolving, quantitatively be transferred in the separatory funnel,, discard ether solution with extracted with diethyl ether 2～3 times, water liquid merges n-butyl alcohol liquid with water-saturated n-butanol extraction 3～5 times, with ammonia solution washing 1～2 time, discard ammoniacal liquor, n-butyl alcohol liquid water bath method, residue add 50% ethanol, 20～30ml dissolving and quantitatively are transferred in the flask, add concentrated hydrochloric acid 1～5ml, boiling water bath hydrolysis 1～3 hour, put cold, with chloroform extraction 2～5 times, combined chloroform liquid, to near neutral, water bath method, residue add dissolve with methanol and quantitatively are transferred in the measuring bottle with water washing, add methanol to scale, shake up, filter with microporous filter membrane, promptly.
Accurate reference substance solution I, II and each the 10 μ l of need testing solution of drawing of algoscopy inject chromatograph of liquid, measure, promptly.
Wherein, as detecting with UV-detector, wavelength is 190nm～230nm; As using evaporative light scattering detector ELSD 800, testing conditions is: 40～60 ℃ of drift tube temperatures, nebulizer gas pressure 3.0～3.2bar; As use evaporative light scattering detector ELSD2000, testing conditions is: 95～125 ℃ of drift tube temperatures, carrier gas flux 1.5～2.5L/min.
The qualitative identification method:
It is an amount of to get the diosgenin reference substance, adds methanol and makes reference substance solution; Test according to thin layer chromatography, draw each 5ul of used need testing solution in reference substance solution and the assay, put respectively in same be on the silica gel g thin-layer plate of adhesive with the sodium carboxymethyl cellulose, with 5～12: toluene-acetone of 1 is developing solvent, launch, take out, dry, spray is with 5～10% ethanol solution of sulfuric acid of 0.5～10% vanillin, and it is clear that hot blast blows to the speckle colour developing.In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the speckle of same color.
The specific embodiment
Direct purpose of the present invention is the preparation Rhizoma Smilacis Chinensis extract, but final purpose is still made Rhizoma Smilacis Chinensis extract certain dosage form, thereby following preparation by several dosage forms illustrates beneficial effect of the present invention.
Embodiment 1:
Preparation Rhizoma Smilacis Chinensis capsule
Get Rhizoma Smilacis Chinensis 7500g, decoct with water three times, each 2 hours, add 8 times of amounts of water, merge decocting liquid, filter, centrifugal (4000rpm/min), filtrate decompression is concentrated into relative density 1.20～1.25 (80 ℃), room temperature to be chilled to, add ethanol and make and contain alcohol amount and reach 40%, left standstill cold preservation 24 hours, filter, decompression filtrate recycling ethanol, and continue to be concentrated into relative density 1.20～1.25 (80 ℃), spray drying (190 ± 5 ℃ of inlet temperature, 100 ± 5 ℃ of leaving air temps); Get dry extract, encapsulated, make 1000 of capsules, promptly.
This product is a capsule, and content is a sepia, feeble QI perfume (or spice), bitter in the mouth, puckery.
Embodiment 2:
Preparation Rhizoma Smilacis Chinensis soft capsule
Get Rhizoma Smilacis Chinensis 7500g, decoct with water secondary, each 3 hours, add 6 times of amounts of water, merge decocting liquid, filter, centrifugal (4000rpm/min), filtrate decompression is concentrated into relative density 1.20～1.25 (80 ℃), room temperature to be chilled to, add ethanol and make and contain alcohol amount and reach 60%, left standstill cold preservation 24 hours, filter, decompression filtrate recycling ethanol, and continue to be concentrated into relative density 1.20～1.25 (80 ℃), spray drying (190 ± 5 ℃ of inlet temperature, 100 ± 5 ℃ of leaving air temps); Get dry extract, adding vegetable oil to total amount is 500g, mix homogeneously, and colloid mill ground 20 minutes, and is encapsulated, makes 1000 of soft capsules, promptly.
This product is a soft capsule, and content is a brown liquid, feeble QI perfume (or spice), bitter in the mouth, puckery.
Embodiment 3:
Preparation Rhizoma Smilacis Chinensis injection
Get Rhizoma Smilacis Chinensis 7500g, decoct with water secondary, each 2 hours, add for the first time 8 times of amounts of water, add for the second time 6 times of amounts of water, merge decocting liquid, filter, centrifugal (4000rpm/min), filtrate decompression is concentrated into relative density 1.20～1.25 (80 ℃), and room temperature to be chilled to adds ethanol and makes and contain alcohol amount and reach 40%, left standstill cold preservation 24 hours, filter, decompression filtrate recycling ethanol, and continue to be concentrated into relative density 1.20～1.25 (80 ℃), after being dissolved in water, with n-butanol extraction 5 times, merge n-butyl alcohol liquid, reclaim thick paste behind the n-butyl alcohol back that is dissolved in water and go up macroporous resin column absorption, with 70% alcoholic solution eluting, collect ethanol liquid, reclaim ethanol, add an amount of water for injection dilution to relative density 1.20～1.25 (80 ℃), the vacuum cellulosic ultrafiltration, promptly.
This product is an injection, and content is a weak yellow liquid.
Embodiment 4:
High performance liquid chromatogram cooperates evaporative light scattering detector to measure the content of the diosgenin in the Rhizoma Smilacis Chinensis soft capsule
Chromatographic condition and system suitability test are filler with octadecylsilane chemically bonded silica; Mobile phase acetonitrile-water (90: 10), flow velocity 1.0ml/min, 40 ℃ of column temperatures.Evaporative light scattering detector (ELSD 800) testing conditions: 40 ℃ of drift tube temperatures, nebulizer gas pressure 3.08bar; Evaporative light scattering detector (ELSD 2000) testing conditions: 105 ℃ of drift tube temperatures, carrier gas flux 2.0L/min.
It is an amount of that the preparation precision of reference substance solution takes by weighing the diosgenin reference substance, adds dissolve with methanol, makes the solution that every 1ml contains diosgenin 0.25mg, in contrast the product solution I; Get the reference substance solution that concentration is 0.25mg/ml, add methanol dilution and make the solution that every 1ml contains diosgenin 0.125mg, product solution II in contrast, promptly.
20 of this product are got in the preparation of need testing solution, and inclining content, takes by weighing 4g, the accurate title, decide, and adds methanol 50ml, supersound extraction (250W, 50KHz) 30 minutes, put coldly, filter, with an amount of methanol wash residue, filter, merge methanol solution, water bath method, residue add water 25ml makes dissolving, quantitatively is transferred in the separatory funnel, with extracted with diethyl ether three times, each 25ml discards ether solution, water liquid is with water-saturated n-butanol extraction four times, and each 25ml merges n-butyl alcohol liquid, with ammonia solution washing secondary, each 40ml discards ammoniacal liquor, n-butyl alcohol liquid water bath method, residue add 50% ethanol 20ml dissolving and quantitatively are transferred in the flask, add concentrated hydrochloric acid 2ml, boiling water bath hydrolysis 2 hours, put cold, with chloroform extraction three times, each 25ml, combined chloroform liquid, extremely near neutral with water washing, water bath method, residue adds dissolve with methanol and quantitatively is transferred in the 5ml measuring bottle, adds methanol to scale, shakes up, filter with microporous filter membrane, promptly.
Accurate reference substance solution I, II and each the 20 μ l of need testing solution of drawing of algoscopy inject chromatograph of liquid, measure, promptly.
Every of this product contains diosgenin (C
27H
42O
3) must not be less than 0.08mg.
Embodiment 5:
Diosgenin in the thin layer chromatography qualitative identification Rhizoma Smilacis Chinensis soft capsule
It is an amount of to get the diosgenin reference substance, adds methanol and makes the solution that every 1ml contains diosgenin 0.2mg, in contrast product solution.Test according to thin layer chromatography, draw each 5ul of need testing solution under reference substance solution and [assay] item, put respectively in same be on the silica gel g thin-layer plate of adhesive with the sodium carboxymethyl cellulose, with toluene-acetone (9: 1) is developing solvent, launch, take out, dry, spray is with 10% ethanol solution of sulfuric acid of 5% vanillin, and it is clear that hot blast blows to the speckle colour developing.In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the speckle of same color.
Embodiment 6:
High performance liquid chromatogram cooperates evaporative light scattering detector to measure the content of the diosgenin in the Rhizoma Smilacis Chinensis injection
Chromatographic condition and system suitability test are filler with octadecylsilane chemically bonded silica; Mobile phase acetonitrile-water (90: 10), flow velocity 1.0ml/min, 40 ℃ of column temperatures.Evaporative light scattering detector (ELSD 800) testing conditions: 40 ℃ of drift tube temperatures, nebulizer gas pressure 3.08bar; Evaporative light scattering detector (ELSD 2000) testing conditions: 105 ℃ of drift tube temperatures, carrier gas flux 2.0L/min.
It is an amount of that the preparation precision of reference substance solution takes by weighing the diosgenin reference substance, adds dissolve with methanol, makes the solution that every 1ml contains diosgenin 0.25mg, in contrast the product solution I; Get the reference substance solution that concentration is 0.25mg/ml, add methanol dilution and make the solution that every 1ml contains diosgenin 0.125mg, product solution II in contrast, promptly.
This product is got in the preparation of need testing solution, and precision is measured 10ml, quantitatively is transferred in the flask, add concentrated hydrochloric acid 2ml, boiling water bath hydrolysis 2 hours is put cold, with chloroform extraction three times, each 25ml, combined chloroform liquid, to near neutral, water bath method, residue add dissolve with methanol and quantitatively are transferred in the 5ml measuring bottle with water washing, add methanol to scale, shake up, filter with microporous filter membrane, promptly.
Accurate reference substance solution I, II and each the 20 μ l of need testing solution of drawing of algoscopy inject chromatograph of liquid, measure, promptly.
Every of this product contains diosgenin (C
27H
42O
3) must not be less than 0.8mg.
Embodiment 7:
Diosgenin in the thin layer chromatography qualitative identification Rhizoma Smilacis Chinensis injection
It is an amount of to get the diosgenin reference substance, adds methanol and makes the solution that every 1ml contains diosgenin 0.2mg, in contrast product solution.Test according to thin layer chromatography, draw each 5ul of need testing solution under reference substance solution and [assay] item, put respectively in same be on the silica gel g thin-layer plate of adhesive with the sodium carboxymethyl cellulose, with toluene-acetone (9: 1) is developing solvent, launch, take out, dry, spray is with 10% ethanol solution of sulfuric acid of 5% vanillin, and it is clear that hot blast blows to the speckle colour developing.In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the speckle of same color.
Claims (11)
1. the preparation method of a Chinese medicine Rhizoma Smilacis Chinensis extract is characterized in that this method comprises following extraction step:
Get the Rhizoma Smilacis Chinensis medical material, add 6～8 times of decoctings and boil 2～3 times, each 2～3 hours, merge decocting liquid, filter, filtrate decompression is concentrated into relative density 1.20～1.25, room temperature to be chilled to, add ethanol and make and contain amount of alcohol and reach 40～60%, left standstill cold preservation 24 hours, filter, decompression filtrate recycling ethanol, and continuing to be concentrated into relative density 1.20～1.25, spray drying obtains medicated powder.
2. preparation method as claimed in claim 1 is characterized in that this method comprises following extraction step:
Get the Rhizoma Smilacis Chinensis medical material, decoct with water 2 times, each 2 hours, add for the first time 6 times of water gagings, add 8 times of water gagings for the second time, merge decocting liquid, filter, relative density 1.20～1.25 when filtrate decompression was concentrated into 80 ℃, room temperature to be chilled to, add ethanol and make and contain amount of alcohol and reach 40%, left standstill cold preservation 24 hours, filter, decompression filtrate recycling ethanol, and relative density 1.20～1.25 when continuing to be concentrated into 80 ℃, spray drying obtains medicated powder.
3. the preparation method of a Chinese medicine Rhizoma Smilacis Chinensis extract is characterized in that this method comprises following extraction step:
Get the Rhizoma Smilacis Chinensis medical material, add 6～8 times of 40%～70% alcohol reflux 2～3 times, each 2～3 hours, merge ethanol liquid, filter, decompression filtrate recycling ethanol, and continue to be concentrated into relative density 1.20～1.25, spray drying obtains medicated powder.
4. preparation method as claimed in claim 3 is characterized in that this method comprises following extraction step:
Get the Rhizoma Smilacis Chinensis medical material, add 6 times of 50% alcohol reflux 2 times, each 2 hours, merge ethanol liquid, filter, decompression filtrate recycling ethanol, and relative density 1.20～1.25 when continuing to be concentrated into 80 ℃, spray drying obtains medicated powder.
5. as the preparation method of claim 2 or 4 described Chinese medicine Rhizoma Smilacis Chinensis extracts, it is characterized in that this method also comprises following process for refining:
After the medicated powder that obtains after spray done is dissolved in water, with n-butanol extraction 4～5 times, merge n-butyl alcohol liquid, go up macroporous resin column absorption after thick paste behind the recovery n-butyl alcohol is dissolved in water, impurity is removed in washing, with 50～70% alcoholic solution eluting, collect ethanol liquid, relative density 1.20～1.25 when reclaiming ethanol to 80 ℃, spray drying obtains Rhizoma Smilacis Chinensis extract.
6. the preparation method of Rhizoma Smilacis Chinensis extract as claimed in claim 5 is characterized in that the concrete purification step in this method is as follows:
After the medicated powder that obtains after spray done is dissolved in water, with n-butanol extraction 5 times, merge n-butyl alcohol liquid, go up macroporous resin column absorption after thick paste behind the recovery n-butyl alcohol is dissolved in water, impurity is removed in washing, with 70% alcoholic solution eluting, collect ethanol liquid, relative density 1.20～1.25 when reclaiming ethanol to 80 ℃, spray drying obtains Rhizoma Smilacis Chinensis extract.
7. must ask the preparation method of 6 described Rhizoma Smilacis Chinensis extracts as power, it is characterized in that used macroporous resin is the polystyrene type macroporous adsorbent resin; Spray-dired condition is 190 ± 5 ℃ of inlet temperature, 100 ± 5 ℃ of leaving air temps.
8. Rhizoma Smilacis Chinensis extract that makes by claim 5,6 or 7 described preparation methoies.
9. as the method for quality control of Rhizoma Smilacis Chinensis extract as described in the claim 8, it is characterized in that comprising in this method following content assaying method:
Chromatographic condition and system suitability test are filler with octadecylsilane chemically bonded silica; Acetonitrile-water of 70～90: 30～10 is mobile phase; Flow velocity 0.8～1.2ml/min, 25～40 ℃ of column temperatures, ultraviolet or evaporative light scattering detector detect;
It is an amount of that the preparation precision of reference substance solution takes by weighing the diosgenin reference substance, adds dissolve with methanol, makes and determine concentration solution, product solution I in contrast; Get reference substance solution I, add 1～2 times of methanol dilution, make reference substance solution II, promptly;
It is fixed that the accurate title of this product is got in the preparation of need testing solution, added methanol extraction 20～30 minutes, puts cold, filter,, filter with an amount of methanol wash residue, merge methanol solution, water bath method, residue add water makes dissolving, quantitatively be transferred in the separatory funnel,, discard ether solution with extracted with diethyl ether 2～3 times, water liquid merges n-butyl alcohol liquid with water-saturated n-butanol extraction 3～5 times, with ammonia solution washing 1～2 time, discard ammoniacal liquor, n-butyl alcohol liquid water bath method, residue add 50% ethanol, 20～30ml dissolving and quantitatively are transferred in the flask, add concentrated hydrochloric acid 1～5ml, boiling water bath hydrolysis 1～3 hour, put cold, with chloroform extraction 2～5 times, combined chloroform liquid, to near neutral, water bath method, residue add dissolve with methanol and quantitatively are transferred in the measuring bottle with water washing, add methanol to scale, shake up, filter with microporous filter membrane, promptly;
Accurate reference substance solution I, II and each the 10 μ l of need testing solution of drawing of algoscopy inject chromatograph of liquid, measure, promptly.
10. method of quality control as claimed in claim 9 is characterized in that it is 190nm～230nm that UV-detector detects wavelength; The testing conditions of evaporative light scattering detector ELSD 800: 40～60 ℃ of drift tube temperatures, nebulizer gas pressure 3.0～3.2bar; The testing conditions of evaporative light scattering detector ELSD 2000: 95～125 ℃ of drift tube temperatures, carrier gas flux 1.5～2.5L/min.
11., it is characterized in that also containing in this method following thin layer chromatography qualitative method as claim 9 or 10 described method of quality control:
It is an amount of to get the diosgenin reference substance, adds methanol and makes reference substance solution; Test according to thin layer chromatography, draw each 5ul of used need testing solution in reference substance solution and the assay, put respectively in same be on the silica gel g thin-layer plate of adhesive with the sodium carboxymethyl cellulose, with 5～12: toluene-acetone of 1 is developing solvent, launch, take out, dry, spray is with 5～10% ethanol solution of sulfuric acid of 0.5～10% vanillin, and it is clear that hot blast blows to the speckle colour developing.In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show the speckle of same color.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101703669B (en) * | 2009-12-15 | 2012-03-28 | 湖北福人药业股份有限公司 | Smilax china effective fractions and extraction as well as purification process thereof |
| CN101549081B (en) * | 2008-03-31 | 2012-09-05 | 桂林三金药业股份有限公司 | Method of quality control for smilax china |
| CN102998270A (en) * | 2012-12-11 | 2013-03-27 | 河南省康星药业股份有限公司 | Method for determining content of total esculentoside |
| CN104407088A (en) * | 2014-12-04 | 2015-03-11 | 天津大学 | Quantitative analysis method for dioscin in compounded traditional Chinese medicine preparation |
| CN110596298A (en) * | 2019-10-15 | 2019-12-20 | 韦必爱 | Quality evaluation method of Yao medicine material Zingiber zerumbet |
-
2005
- 2005-05-11 CN CN 200510200269 patent/CN1861180A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101549081B (en) * | 2008-03-31 | 2012-09-05 | 桂林三金药业股份有限公司 | Method of quality control for smilax china |
| CN101703669B (en) * | 2009-12-15 | 2012-03-28 | 湖北福人药业股份有限公司 | Smilax china effective fractions and extraction as well as purification process thereof |
| CN102998270A (en) * | 2012-12-11 | 2013-03-27 | 河南省康星药业股份有限公司 | Method for determining content of total esculentoside |
| CN104407088A (en) * | 2014-12-04 | 2015-03-11 | 天津大学 | Quantitative analysis method for dioscin in compounded traditional Chinese medicine preparation |
| CN110596298A (en) * | 2019-10-15 | 2019-12-20 | 韦必爱 | Quality evaluation method of Yao medicine material Zingiber zerumbet |
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Open date: 20061115 |