CN102552751A - Quality detection method for medicine (named as Er bao concentrated decoction) - Google Patents
Quality detection method for medicine (named as Er bao concentrated decoction) Download PDFInfo
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- CN102552751A CN102552751A CN201010610114XA CN201010610114A CN102552751A CN 102552751 A CN102552751 A CN 102552751A CN 201010610114X A CN201010610114X A CN 201010610114XA CN 201010610114 A CN201010610114 A CN 201010610114A CN 102552751 A CN102552751 A CN 102552751A
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Abstract
The invention belongs to the field of pharmacy and relates to a detection method for Chinese herbal preparation of medicine (named as Er bao concentrated decoction), in particular to a detection method for the medicine (named as Er bao concentrated decoction). The detection method uses a thin layer chromatography method to identify radix pseudostellariae, poria cocos, Chinese yam and radia paeoniae alba in products and adopts a high efficiency liquid chromatography method to measure the content of paeoniflorin which is a main constituent of the radia paeoniae alba. The detection method is quick in speed, high in accuracy and capable of effectively controlling quality of the medicine (named as Er bao concentrated decoction).
Description
Technical field
The present invention relates to a kind of detection method of Chinese medicine preparation, be specifically related to the detection method of the precious cream of a kind of youngster, belong to field of traditional Chinese medicine pharmacy.
Background technology
The precious cream of youngster is prepared from Chinese crude drug Radix Pseudostellariae, Radix Glehniae, Poria, Rhizoma Dioscoreae, Fructus Crataegi, Fructus Hordei Germinatus, Semen Lablab Album, Pericarpium Citri Reticulatae, the Radix Paeoniae Alba, Radix Ophiopogonis and the Radix Puerariae common process by the Chinese patent medicine mixture.Product has invigorating the spleen and benefiting QI, the appetizing effect of promoting the production of body fluid, and it is weak to be used for children's's yellowish complexion, indigestion and loss of appetite anorexia, insufficiency of the spleen chronic diarrhea, lassitude, dry mouth is thirsty, diseases such as night sweat.
The precious cream prescription of youngster:
Radix Pseudostellariae 120g, Radix Glehniae 75g, Poria 120g, Rhizoma Dioscoreae 120g, Fructus Crataegi (stir-fry) 45g, Fructus Hordei Germinatus (stir-fry) 45g, Semen Lablab Album (stir-fry) 120g, Pericarpium Citri Reticulatae 45g, the Radix Paeoniae Alba (stir-fry) 45g, Radix Ophiopogonis 45g, Radix Puerariae (stewing) 45g.
Method for making: above ten simply, and the decocte with water secondary 4 hours for the first time, 3 hours for the second time, filters; Merging filtrate leaves standstill, and getting supernatant concentration to relative density is the clear paste of 1.17 ~ 1.19 (85 ℃), and other gets maltose 250g heated and boiled; Filter, use sucrose 200g, process the conversion liquid glucose, add citric acid 3g by every 100g clear paste; Add above-mentioned clear paste after stirring again,, be concentrated into the relative density of regulation, promptly get with the liquid glucose mixing.
The precious cream standard of youngster WS
3Have only peoniflorin thin layer chromatography discrimination method among the-B-3128-98, and the method that has no active constituent content to detect, can not fine control product quality.
Summary of the invention
To the defective that existing detection method exists, the present invention has increased the discriminating of product effective ingredient and the method for assay in the proper mass detection method, solved quality Control in the process of producing product effectively.
The present invention realizes through following method:
(1) adopting thin layer chromatography, is control medicinal material with the Radix Pseudostellariae, differentiates in the precious cream of youngster to contain the Radix Pseudostellariae medical material;
(2) adopting thin layer chromatography, is control medicinal material with the Poria, differentiates in the precious cream of youngster to contain the Poria medical material;
(3) adopting thin layer chromatography, is control medicinal material with the Rhizoma Dioscoreae, differentiates in the precious cream of youngster to contain the Rhizoma Dioscoreae medical material;
(4) adopt content of paeoniflorin in the precious cream of high effective liquid chromatography for measuring, peoniflorin is one of medical material Radix Paeoniae Alba Main Ingredients and Appearance.
The precious cream detection method of youngster of the present invention, wherein discrimination method may further comprise the steps:
Thin layer chromatography is adopted in said discriminating, is control medicinal material with Radix Pseudostellariae, Poria, Rhizoma Dioscoreae, differentiates the ingredient in the precious cream of youngster;
Said assay adopts content of paeoniflorin in the precious cream of high effective liquid chromatography for measuring.
The detection method of the precious cream of youngster of the present invention, wherein discrimination method may further comprise the steps:
(1) get the precious cream 30ml of youngster, add the equivalent methanol eddy and extracted 30 minutes, put coldly, filter, the filtrating water-bath is concentrated into about 2ml, as need testing solution; Other gets Radix Pseudostellariae control medicinal material 1g, adds methanol 10ml, warm macerating, and jolting 30 minutes filters, and filtrating is concentrated into 1ml, as control medicinal material solution; According to thin layer chromatography (an appendix VI of version pharmacopeia in 2010 B), draw each 5 μ l of above-mentioned two kinds of solution, put respectively in same be on the silica gel g thin-layer plate of adhesive with the sodium carboxymethyl cellulose; (4:1:1) is developing solvent with n-butyl alcohol-glacial acetic acid-water, puts with in 15 minutes the expansion cylinder of developing solvent presaturation, launches; Take out, dry, spray is with 0.2% ethanol solution of ninhydrin; It is clear to be heated to speckle colour developing at 105 ℃; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the speckle of same color.
(2) get the precious cream 30ml of youngster, add the equivalent aether backflow and extracted 30 minutes, put coldly, filter, the filtrating evaporate to dryness, residue adds methanol 1ml makes dissolving, as need testing solution; Other gets Poria control medicinal material 1g, the 50ml that adds diethyl ether, and supersound process 10 minutes filters, the filtrating evaporate to dryness, residue adds methanol 1ml makes dissolving, as control medicinal material solution; According to thin layer chromatography (an appendix VI of version pharmacopeia in 2010 B), draw each 5 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate; With toluene-ethyl acetate-formic acid (20:5:0.5) is developing solvent, launches, and takes out; Dry, spray is with 2% vanillin sulphuric acid-ethanol (4:1) mixed solution, and it is clear to be heated to speckle colour developing at 105 ℃; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the principal spot of same color.
(3) get the precious cream 30ml of youngster, add the equivalent methylene chloride reflux and extracted 1 hour, filter, filtrating evaporate to dryness, the residue 1ml that adds methylene chloride makes dissolving, as need testing solution; Other gets Rhizoma Dioscoreae control medicinal material 5g, the 30ml that adds methylene chloride, and reflux 2 hours filters, and filtrating evaporate to dryness, the residue 1ml that adds methylene chloride makes dissolving, as control medicinal material solution; According to thin layer chromatography (an appendix VI of version pharmacopeia in 2010 B), draw each 4 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate; With ethyl acetate-methanol-strong ammonia solution (9: 1: 0.5) is developing solvent, launches, and takes out; Dry, spray is with 10% phosphomolybdic acid ethanol solution, and it is clear to be heated to speckle colour developing at 105 ℃; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the speckle of same color.
The detection method of the precious cream of youngster of the present invention, wherein assay may further comprise the steps:
The preparation of reference substance solution: it is an amount of to get the peoniflorin reference substance, and accurate the title decides, and adds 50% methanol and processes the solution that every 1ml contains 0.1mg;
The preparation of need testing solution: get these article 10ml, through D
101On the macroporous adsorptive resins (internal diameter 1.5cm, column length 10cm),, discard water liquid with water 50ml eluting; Reuse 50% methanol 50ml eluting is collected eluent, evaporate to dryness; Residue adds 50% methanol makes dissolving, and is transferred in the 25ml measuring bottle, adds 50% methanol to scale; Shake up, filter, get subsequent filtrate with microporous filter membrane (0.45 μ m);
Condition determination: chromatographic condition and system suitability test: use octadecylsilane chemically bonded silica to be filler; Methanol-0.2% phosphoric acid (25:75) is mobile phase; Detect wavelength 230nm.Number of theoretical plate calculates by the peoniflorin peak should be not less than 3000;
Algoscopy: accurate respectively reference substance solution and each 10 μ l of need testing solution of drawing, inject chromatograph of liquid, measure, the every 1ml of these article contains the Radix Paeoniae Alba with peoniflorin (C
23H
28O
11) meter, must not be less than 0.4mg.
The specific embodiment
Embodiment 1
Prescription: Radix Pseudostellariae 120g, Radix Glehniae 75g, Poria 120g, Rhizoma Dioscoreae 120g, Fructus Crataegi (stir-fry) 45g, Fructus Hordei Germinatus (stir-fry) 45g, Semen Lablab Album (stir-fry) 120g, Pericarpium Citri Reticulatae 45g, the Radix Paeoniae Alba (stir-fry) 45g, Radix Ophiopogonis 45g, Radix Puerariae (stewing) 45g.
Method for making: above ten simply, and the decocte with water secondary 4 hours for the first time, 3 hours for the second time, filters; Merging filtrate leaves standstill, and getting supernatant concentration to relative density is the clear paste of 1.17 ~ 1.19 (85 ℃), and other gets maltose 250g heated and boiled; Filter, use sucrose 200g, process the conversion liquid glucose, add citric acid 3g by every 100g clear paste; Add above-mentioned clear paste after stirring again,, be concentrated into the relative density of regulation, promptly get with the liquid glucose mixing.
The quality determining method of the precious cream of youngster is:
Differentiate:
(1) get the precious cream 30ml of youngster, add the equivalent methanol eddy and extracted 30 minutes, put coldly, filter, the filtrating water-bath is concentrated into about 2ml, as need testing solution; Other gets Radix Pseudostellariae control medicinal material 1g, adds methanol 10ml, warm macerating, and jolting 30 minutes filters, and filtrating is concentrated into 1ml, as control medicinal material solution; According to thin layer chromatography (an appendix VI of version pharmacopeia in 2010 B), draw each 5 μ l of above-mentioned two kinds of solution, put respectively in same be on the silica gel g thin-layer plate of adhesive with the sodium carboxymethyl cellulose; (4:1:1) is developing solvent with n-butyl alcohol-glacial acetic acid-water, puts with in 15 minutes the expansion cylinder of developing solvent presaturation, launches; Take out, dry, spray is with 0.2% ethanol solution of ninhydrin; It is clear to be heated to speckle colour developing at 105 ℃; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the speckle of same color.
(2) get the precious cream 30ml of youngster, add the equivalent aether backflow and extracted 30 minutes, put coldly, filter, the filtrating evaporate to dryness, residue adds methanol 1ml makes dissolving, as need testing solution; Other gets Poria control medicinal material 1g, the 50ml that adds diethyl ether, and supersound process 10 minutes filters, the filtrating evaporate to dryness, residue adds methanol 1ml makes dissolving, as control medicinal material solution; According to thin layer chromatography (an appendix VI of version pharmacopeia in 2010 B), draw each 5 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate; With toluene-ethyl acetate-formic acid (20:5:0.5) is developing solvent, launches, and takes out; Dry, spray is with 2% vanillin sulphuric acid-ethanol (4:1) mixed solution, and it is clear to be heated to speckle colour developing at 105 ℃; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the principal spot of same color.
(3) get the precious cream 30ml of youngster, add the equivalent methylene chloride reflux and extracted 1 hour, filter, filtrating evaporate to dryness, the residue 1ml that adds methylene chloride makes dissolving, as need testing solution; Other gets Rhizoma Dioscoreae control medicinal material 5g, the 30ml that adds methylene chloride, and reflux 2 hours filters, and filtrating evaporate to dryness, the residue 1ml that adds methylene chloride makes dissolving, as control medicinal material solution; According to thin layer chromatography (an appendix VI of version pharmacopeia in 2010 B), draw each 4 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate; With ethyl acetate-methanol-strong ammonia solution (9: 1: 0.5) is developing solvent, launches, and takes out; Dry, spray is with 10% phosphomolybdic acid ethanol solution, and it is clear to be heated to speckle colour developing at 105 ℃; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the speckle of same color.
(4) get the precious cream 30ml of youngster, add water 20ml, mixing, with water saturated n-butanol extraction 2 times, each 50ml merges n-butyl alcohol liquid, with 40% ammonia scrubbing 2 times, 50ml at every turn, n-butyl alcohol liquid evaporate to dryness adds ethanol 1ml and makes dissolving, as need testing solution; Other gets the peoniflorin reference substance, adds ethanol and processes the solution that every 1ml contains 1mg, as reference substance solution; According to thin layer chromatography (an appendix VI of version pharmacopeia in 2010 B), draw each 10 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate; With chloroform-ethyl acetate-methanol-formic acid (40:5:10:0.2) is developing solvent, launches, and takes out; Dry, spray is with 10% sulfuric acid solution of 5% vanillin, and it is clear that hot blast blows to the speckle colour developing; In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show identical aubergine speckle.
Assay:
Take high picture liquid chromatography (an appendix VI of Chinese Pharmacopoeia version in 2005 D), chromatographic condition and system suitability test chromatographic condition and system suitability test: use octadecylsilane chemically bonded silica to be filler; Methanol-0.2% phosphoric acid (25:75) is mobile phase; Detect wavelength 230nm.Number of theoretical plate calculates by the peoniflorin peak should be not less than 3000;
The preparation of reference substance solution: it is an amount of to get the peoniflorin reference substance, and accurate the title decides, and adds 50% methanol and processes the solution that every 1ml contains 0.1mg;
The preparation of need testing solution: get the precious cream 10ml of youngster, through D
101On the macroporous adsorptive resins (internal diameter 1.5cm, column length 10cm),, discard water liquid with water 50ml eluting; Reuse 50% methanol 50ml eluting is collected eluent, evaporate to dryness; Residue adds 50% methanol makes dissolving, and is transferred in the 25ml measuring bottle, adds 50% methanol to scale; Shake up, filter, get subsequent filtrate with microporous filter membrane (0.45 μ m);
Algoscopy: accurate respectively reference substance solution and each 10 μ l of need testing solution of drawing, inject chromatograph of liquid, measure, promptly get.Every 1ml contains the Radix Paeoniae Alba with peoniflorin (C
23H
28O
11) meter, must not be less than 0.4mg.
Claims (8)
1. the detection method of the precious cream of youngster is characterized in that, comprises the discriminating and the assay of product active ingredient.
2. according to the detection method of claim 1, it is characterized in that, adopt thin layer chromatography to differentiate the Radix Pseudostellariae in the product, Poria, Rhizoma Dioscoreae, Radix Paeoniae Alba composition adopt main one-tenth content of paeoniflorin in the high effective liquid chromatography for measuring Radix Paeoniae Alba.
3. detection method according to claim 2 is characterized in that discrimination method may further comprise the steps: get the precious cream 30ml of youngster, add the equivalent methanol eddy and extracted 30 minutes, put coldly, filter, the filtrating water-bath is concentrated into about 2ml, as need testing solution; Other gets Radix Pseudostellariae control medicinal material 1g, adds methanol 10ml, warm macerating, and jolting 30 minutes filters, and filtrating is concentrated into 1ml, as control medicinal material solution; According to thin layer chromatography (an appendix VI of version pharmacopeia in 2010 B), draw each 5 μ l of above-mentioned two kinds of solution, put respectively in same be on the silica gel g thin-layer plate of adhesive with the sodium carboxymethyl cellulose; (4:1:1) is developing solvent with n-butyl alcohol-glacial acetic acid-water, puts with in 15 minutes the expansion cylinder of developing solvent presaturation, launches; Take out, dry, spray is with 0.2% ethanol solution of ninhydrin; It is clear to be heated to speckle colour developing at 105 ℃; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the speckle of same color.
4. detection method according to claim 2 is characterized in that discrimination method may further comprise the steps: gets the precious cream 30ml of youngster, adds the equivalent aether backflow and extracted 30 minutes, put coldly, filter, and the filtrating evaporate to dryness, residue adds methanol 1ml makes dissolving, as need testing solution; Other gets Poria control medicinal material 1g, the 50ml that adds diethyl ether, and supersound process 10 minutes filters, the filtrating evaporate to dryness, residue adds methanol 1ml makes dissolving, as control medicinal material solution; According to thin layer chromatography (an appendix VI of version pharmacopeia in 2010 B), draw each 5 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate; With toluene-ethyl acetate-formic acid (20:5:0.5) is developing solvent, launches, and takes out; Dry, spray is with 2% vanillin sulphuric acid-ethanol (4:1) mixed solution, and it is clear to be heated to speckle colour developing at 105 ℃; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the principal spot of same color.
5. detection method according to claim 2 is characterized in that discrimination method may further comprise the steps: get the precious cream 30ml of youngster, add the equivalent methylene chloride reflux and extracted 1 hour, filter, filtrating evaporate to dryness, the residue 1ml that adds methylene chloride makes dissolving, as need testing solution; Other gets Rhizoma Dioscoreae control medicinal material 5g, the 30ml that adds methylene chloride, and reflux 2 hours filters, and filtrating evaporate to dryness, the residue 1ml that adds methylene chloride makes dissolving, as control medicinal material solution; According to thin layer chromatography (an appendix VI of version pharmacopeia in 2010 B), draw each 4 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate; With ethyl acetate-methanol-strong ammonia solution (9: 1: 0.5) is developing solvent, launches, and takes out; Dry, spray is with 10% phosphomolybdic acid ethanol solution, and it is clear to be heated to speckle colour developing at 105 ℃; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the speckle of same color.
6. detection method according to claim 2 is characterized in that discrimination method may further comprise the steps: get the precious cream 30ml of youngster, add water 20ml; Mixing, with water saturated n-butanol extraction 2 times, each 50ml; Merge n-butyl alcohol liquid, with 40% ammonia scrubbing 2 times, 50ml at every turn; N-butyl alcohol liquid evaporate to dryness adds ethanol 1ml and makes dissolving, as need testing solution; Other gets the peoniflorin reference substance, adds ethanol and processes the solution that every 1ml contains 1mg, as reference substance solution; According to thin layer chromatography (an appendix VI of version pharmacopeia in 2010 B), draw each 10 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate; With chloroform-ethyl acetate-methanol-formic acid (40:5:10:0.2) is developing solvent, launches, and takes out; Dry, spray is with 10% sulfuric acid solution of 5% vanillin, and it is clear that hot blast blows to the speckle colour developing; In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show identical aubergine speckle.
7. detection method according to claim 2, wherein assay may further comprise the steps:
It is an amount of to get the peoniflorin reference substance, and accurate the title decides, and adds 50% methanol and processes the solution that every 1ml contains 0.1mg, gets the precious cream 10ml of youngster, through D
101On the macroporous adsorptive resins (internal diameter 1.5cm, column length 10cm),, discard water liquid, reuse 50% methanol 50ml eluting with water 50ml eluting; Collect eluent, evaporate to dryness, residue add 50% methanol makes dissolving, and is transferred in the 25ml measuring bottle; Add 50% methanol to scale, shake up, filter, get subsequent filtrate with microporous filter membrane (0.45 μ m); Accurate respectively reference substance solution and each 10 μ l of need testing solution of drawing inject chromatograph of liquid, measure, and the every 1ml of these article contains the Radix Paeoniae Alba with peoniflorin (C
23H
28O
11) meter, must not be less than 0.4mg.
8. detection method according to claim 2, step is following:
(1) get the precious cream 30ml of youngster, add the equivalent methanol eddy and extracted 30 minutes, put coldly, filter, the filtrating water-bath is concentrated into about 2ml, as need testing solution; Other gets Radix Pseudostellariae control medicinal material 1g, adds methanol 10ml, warm macerating, and jolting 30 minutes filters, and filtrating is concentrated into 1ml, as control medicinal material solution; According to thin layer chromatography (an appendix VI of version pharmacopeia in 2010 B), draw each 5 μ l of above-mentioned two kinds of solution, put respectively in same be on the silica gel g thin-layer plate of adhesive with the sodium carboxymethyl cellulose; (4:1:1) is developing solvent with n-butyl alcohol-glacial acetic acid-water, puts with in 15 minutes the expansion cylinder of developing solvent presaturation, launches; Take out, dry, spray is with 0.2% ethanol solution of ninhydrin; It is clear to be heated to speckle colour developing at 105 ℃; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the speckle of same color.
(2) get the precious cream 30ml of youngster, add the equivalent aether backflow and extracted 30 minutes, put coldly, filter, the filtrating evaporate to dryness, residue adds methanol 1ml makes dissolving, as need testing solution; Other gets Poria control medicinal material 1g, the 50ml that adds diethyl ether, and supersound process 10 minutes filters, the filtrating evaporate to dryness, residue adds methanol 1ml makes dissolving, as control medicinal material solution; According to thin layer chromatography (an appendix VI of version pharmacopeia in 2010 B), draw each 5 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate; With toluene-ethyl acetate-formic acid (20:5:0.5) is developing solvent, launches, and takes out; Dry, spray is with 2% vanillin sulphuric acid-ethanol (4:1) mixed solution, and it is clear to be heated to speckle colour developing at 105 ℃; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the principal spot of same color.
(3) get the precious cream 30ml of youngster, add the equivalent methylene chloride reflux and extracted 1 hour, filter, filtrating evaporate to dryness, the residue 1ml that adds methylene chloride makes dissolving, as need testing solution; Other gets Rhizoma Dioscoreae control medicinal material 5g, the 30ml that adds methylene chloride, and reflux 2 hours filters, and filtrating evaporate to dryness, the residue 1ml that adds methylene chloride makes dissolving, as control medicinal material solution; According to thin layer chromatography (an appendix VI of version pharmacopeia in 2010 B), draw each 4 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate; With ethyl acetate-methanol-strong ammonia solution (9: 1: 0.5) is developing solvent, launches, and takes out; Dry, spray is with 10% phosphomolybdic acid ethanol solution, and it is clear to be heated to speckle colour developing at 105 ℃; In the test sample chromatograph, with the corresponding position of control medicinal material chromatograph on, show the speckle of same color.
(4) get the precious cream 30ml of youngster, add water 20ml, mixing, with water saturated n-butanol extraction 2 times, each 50ml merges n-butyl alcohol liquid, with 40% ammonia scrubbing 2 times, 50ml at every turn, n-butyl alcohol liquid evaporate to dryness adds ethanol 1ml and makes dissolving, as need testing solution; Other gets the peoniflorin reference substance, adds ethanol and processes the solution that every 1ml contains 1mg, as reference substance solution; According to thin layer chromatography (an appendix VI of version pharmacopeia in 2010 B), draw each 10 μ l of above-mentioned two kinds of solution, put respectively on same silica gel g thin-layer plate; With chloroform-ethyl acetate-methanol-formic acid (40:5:10:0.2) is developing solvent, launches, and takes out; Dry, spray is with 10% sulfuric acid solution of 5% vanillin, and it is clear that hot blast blows to the speckle colour developing; In the test sample chromatograph, with the corresponding position of reference substance chromatograph on, show identical aubergine speckle.
(5) it is an amount of to get the peoniflorin reference substance, accurately claims surely, adds 50% methanol and processes the solution that every 1ml contains 0.1mg, gets the precious cream 10ml of youngster, through D
101On the macroporous adsorptive resins (internal diameter 1.5cm, column length 10cm),, discard water liquid, reuse 50% methanol 50ml eluting with water 50ml eluting; Collect eluent, evaporate to dryness, residue add 50% methanol makes dissolving, and is transferred in the 25ml measuring bottle; Add 50% methanol to scale, shake up, filter, get subsequent filtrate with microporous filter membrane (0.45 μ m); Accurate respectively reference substance solution and each 10 μ l of need testing solution of drawing inject chromatograph of liquid, measure, and the every 1ml of these article contains the Radix Paeoniae Alba with peoniflorin (C
23H
28O
11) meter, must not be less than 0.4mg.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108287211A (en) * | 2018-01-25 | 2018-07-17 | 贵州广济堂药业有限公司 | A kind of quality determining method of radix pseudostellariae broken wall medicine materical crude slice |
| CN114062553A (en) * | 2021-11-17 | 2022-02-18 | 荣昌制药(淄博)有限公司 | Method for measuring HPLC fingerprint of child six-ingredient appetite increasing ointment |
| CN114324724A (en) * | 2021-12-20 | 2022-04-12 | 河南省奥林特药业有限公司 | Method for identifying authenticity of liver essence paste based on thin-layer chromatography |
-
2010
- 2010-12-29 CN CN201010610114XA patent/CN102552751A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108287211A (en) * | 2018-01-25 | 2018-07-17 | 贵州广济堂药业有限公司 | A kind of quality determining method of radix pseudostellariae broken wall medicine materical crude slice |
| CN114062553A (en) * | 2021-11-17 | 2022-02-18 | 荣昌制药(淄博)有限公司 | Method for measuring HPLC fingerprint of child six-ingredient appetite increasing ointment |
| CN114062553B (en) * | 2021-11-17 | 2024-05-07 | 荣昌制药(淄博)有限公司 | HPLC fingerprint determination method of children six-ingredient food-enhancing ointment |
| CN114324724A (en) * | 2021-12-20 | 2022-04-12 | 河南省奥林特药业有限公司 | Method for identifying authenticity of liver essence paste based on thin-layer chromatography |
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Application publication date: 20120711 |