CN1787739B - 精子处理系统 - Google Patents

精子处理系统 Download PDF

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Publication number
CN1787739B
CN1787739B CN038174847A CN03817484A CN1787739B CN 1787739 B CN1787739 B CN 1787739B CN 038174847 A CN038174847 A CN 038174847A CN 03817484 A CN03817484 A CN 03817484A CN 1787739 B CN1787739 B CN 1787739B
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Prior art keywords
sperm
seminal fluid
temperature
separated
hatched
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Expired - Lifetime
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CN038174847A
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Chinese (zh)
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CN1787739A (zh
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阿利森·C·林赛
约翰·L·申克
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XY LLC
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XY LLC
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0612—Germ cells sorting of gametes, e.g. according to sex or motility
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0278—Physical preservation processes
    • A01N1/0284—Temperature processes, i.e. using a designated change in temperature over time
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/52—Sperm; Prostate; Seminal fluid; Leydig cells of testes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Environmental Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Reproductive Health (AREA)
  • Dentistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN038174847A 2002-07-22 2003-07-22 精子处理系统 Expired - Lifetime CN1787739B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US40048602P 2002-07-22 2002-07-22
US60/400,486 2002-07-22
PCT/US2003/022906 WO2004009237A2 (en) 2002-07-22 2003-07-22 Sperm cell process system

Publications (2)

Publication Number Publication Date
CN1787739A CN1787739A (zh) 2006-06-14
CN1787739B true CN1787739B (zh) 2011-03-30

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN038174847A Expired - Lifetime CN1787739B (zh) 2002-07-22 2003-07-22 精子处理系统

Country Status (10)

Country Link
US (1) US20060067916A1 (ja)
EP (2) EP1542529A4 (ja)
JP (2) JP2005533501A (ja)
CN (1) CN1787739B (ja)
AU (1) AU2003259205B2 (ja)
BR (2) BR0312843A (ja)
CA (1) CA2531176C (ja)
MX (1) MXPA05000865A (ja)
NZ (1) NZ538265A (ja)
WO (1) WO2004009237A2 (ja)

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US7208265B1 (en) 1999-11-24 2007-04-24 Xy, Inc. Method of cryopreserving selected sperm cells
EP2258169B1 (en) 2000-05-09 2019-07-10 Xy, Llc Method for isolating X-chromosome bearing and Y-chromosome bearing populations of spermatozoa
AU2002237689B2 (en) 2000-11-29 2008-01-10 Xy, Llc. System to separate frozen-thawed spermatozoa into X-chromosome bearing and Y-chromosome bearing populations
US7713687B2 (en) 2000-11-29 2010-05-11 Xy, Inc. System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
US8211629B2 (en) 2002-08-01 2012-07-03 Xy, Llc Low pressure sperm cell separation system
US8486618B2 (en) 2002-08-01 2013-07-16 Xy, Llc Heterogeneous inseminate system
MXPA05001654A (es) 2002-08-15 2005-10-18 Xy Inc Citometro de flujo de alta resolucion.
US7169548B2 (en) 2002-09-13 2007-01-30 Xy, Inc. Sperm cell processing and preservation systems
EP1608963B1 (en) 2003-03-28 2009-12-30 Inguran, LLC Apparatus and methods for providing sex-sorted animal sperm
NZ544103A (en) * 2003-05-15 2010-10-29 Xy Llc Efficient haploid cell sorting for flow cytometer systems
WO2005095960A1 (en) 2004-03-29 2005-10-13 Monsanto Technology Llc Use of a composition which regulates regulates oxidation/reduction reactions intracellularly and/or extracellularly in a staining or sorting process of spermatozoa
EP1730266A2 (en) 2004-03-29 2006-12-13 Monsanto Technology, LLC Sperm suspensions for sorting into x or y chromosome-bearing enriched populations
BRPI0513685A (pt) 2004-07-22 2008-05-13 Monsanto Technology Llc processo para enriquecimento de uma população de células de esperma
CN101322489B (zh) * 2008-08-01 2011-11-30 内蒙古蒙牛繁育生物技术有限公司 一种鹿x/y精子分离冷冻精液及其生产方法和应用
CN101671651B (zh) * 2008-09-09 2013-05-29 上海交通大学医学院附属第九人民医院 精子的冷冻和解冻方法及精子的冷冻和解冻装置
KR101005022B1 (ko) * 2009-05-22 2010-12-30 한국화학융합시험연구원 실험동물의 정액 채취 방법 및 이를 이용한 인공수정 방법
WO2011123166A2 (en) * 2010-04-01 2011-10-06 Inguran, Llc Methods and systems for reducing dna fragmentation in a processed sperm sample
EP2556146A4 (en) * 2010-04-01 2013-08-14 Inguran Llc METHOD AND SYSTEMS FOR REDUCING DNA FRAGMENTATION IN A PROCESSED SPERM PROBE
DK2761275T3 (en) 2011-09-30 2017-09-25 Inguran Llc METHODS FOR COLORING AND SORTING SPERMA
US10620213B2 (en) 2012-10-05 2020-04-14 Inguran, Llc High pressure sperm sorting and flow cytometer methods
TR201909835T4 (tr) 2012-10-05 2019-07-22 Inguran Llc Cinsiyet ayıklama için spermlere muamele etme yöntemleri.
CN104630137B (zh) * 2015-01-26 2017-10-20 中国农业科学院北京畜牧兽医研究所 一种猪icsi操作的方法
US9957548B2 (en) 2015-03-30 2018-05-01 General Electric Company Methods of capturing sperm nucleic acids
US10030241B2 (en) 2015-03-30 2018-07-24 General Electric Company Methods and kits for capturing sperm nucleic acids
CN105325401B (zh) * 2015-11-10 2017-12-12 天津市畜牧兽医研究所 一种猪鲜精保存长效稀释粉及制备方法与应用
CN105850985A (zh) * 2016-05-31 2016-08-17 山东鑫基牧业有限公司 一种猪冷冻精液稀释粉
CN106070184A (zh) * 2016-05-31 2016-11-09 山东鑫基牧业有限公司 一种猪精液的冷冻和解冻方法
AU2018365065A1 (en) * 2017-11-10 2020-07-02 Cytolutions, Llc Sexed sperm bulk separation systems

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CN1443519A (zh) * 2002-03-11 2003-09-24 刘凤鸣 一种动物精子的性别分选方法

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US20060067916A1 (en) 2006-03-30
CN1787739A (zh) 2006-06-14
EP1542529A4 (en) 2006-08-09
WO2004009237A2 (en) 2004-01-29
JP2011250793A (ja) 2011-12-15
MXPA05000865A (es) 2005-04-28
EP2301333A1 (en) 2011-03-30
CA2531176A1 (en) 2004-01-29
BR0312843A (pt) 2005-05-10
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