CN1733908A - Abalone triploid pharmaceutical induction method - Google Patents
Abalone triploid pharmaceutical induction method Download PDFInfo
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- CN1733908A CN1733908A CNA2005100468370A CN200510046837A CN1733908A CN 1733908 A CN1733908 A CN 1733908A CN A2005100468370 A CNA2005100468370 A CN A2005100468370A CN 200510046837 A CN200510046837 A CN 200510046837A CN 1733908 A CN1733908 A CN 1733908A
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Abstract
The invention discloses a method for inducing triploid of Haliotis discus Hannai through medicament, which comprises the following steps, preparing 6-dimethoxy aminopurine solution, obtaining abalone sperms and ova through the conventional method, obtaining oosperm through artificial insemination, placing oosperm of seed abalone into sea water, finally inhibiting the second polar body of the Haliotis discus Hannai with 6-dimethoxy aminopurine solution.
Description
Technical field:
The present invention relates to a kind of method of cultivation of haliotis discus hannai Ino new variety, especially a kind of method of abalone triploid pharmaceutical induction.
Background technology:
Bao is a kind of of seashells, once is described as one of marine products eight delicacies.The haliotis diversicolor Reeve fish (H.diversicolor supertexta) on ground such as haliotis discus hannai Ino (Haliotis discushannai), Fujian, Guangdong, Taiwan, Hainan that originates in ground such as China Liaoning, Shandong is the most well-known, its haliotis discus hannai Ino is called as " soft gold ", and price per ton in the international market reaches 4～60,000 dollars.
China since the seventies artificial propagation of haliotis discus hannai Ino or aquaculture research, carry out artificial increasing cultivation's work the eighties on Liaoning, Shandong and other places, obtained gratifying achievement.Whole nation Bao annual production has reached more than 3000 tons about 1,000,000,000 yuan of the output value.But begin the beginning of the nineties, the landslide phenomenon appears in the aquaculture of Bao, mainly be since for many years manually from numerous cause seed downgrade, artificial breeding during young Bao peel off back mass mortality, unit surface and overall seedling rate reduces, use seedling thereby had a strong impact on to form, culture into Bao simultaneously and the phenomena of mortality also occur.
Ploidy operation is an effective way of hydrobiont cell engineering breeding, since 1956 induce three quil fishes to obtain triploid first, has carried out the research of triploid induction on surplus surplus in the of the 20 kinds of fish, 30 kinds of shellfishes and 3 kinds of crustaceans.Up to the present, carrying out the ploidy method of operating mainly contains following several: a kind of is to handle zygote with physical methods such as hot and cold shock and hydrostatic platen presses; Another kind is to handle the embryo who grows different times with chemical agent.Because the abalone culture cycle is longer, the triploid breeding technique is one of one preferred technique of Bao artificial breeding technique.Since 1986, also the begin one's study triploid of Bao of China mainly was the polyploid that adopts cytochalasin B (CB) and temperature shock method to induce Bao, but poor effect, subject matter is times change rate instability and embryo's abnormal rate height.
Summary of the invention:
The present invention is in order to solve the unstable and embryo's abnormal rate high-technology problem of doubly change rate of existing in prior technology, a kind of method that suppresses the abalone triploid pharmaceutical induction of second polar body with 6-dimethylaminopurine solution-treated haliotis discus hannai Ino to be provided.
Technical solution of the present invention is: a kind of method of abalone triploid pharmaceutical induction comprises the steps:
A. produce 6-dimethylaminopurine solution;
B. obtain sperm and the ovum of abalone with ordinary method, obtain zygote with artificial insemination method;
C. zygote is placed seawater, suppress the haliotis discus hannai Ino second polar body with 6-dimethylaminopurine solution, the ultimate density of 6-dimethylaminopurine solution is 75～150 μ mol/l, and time of origin is that first polar body occurs at 30～50% o'clock, and the time length is 15～20min.
The solute of the described 6-of producing dimethylaminopurine solution is distilled water or dimethyl sulfoxide (DMSO).
Described described water temperature is preferably 24～25 ℃, and the ultimate density of 6-dimethylaminopurine solution is preferably 150 μ mol/l, and the time length is preferably 20min.
The present invention suppresses the haliotis discus hannai Ino second polar body with 6-dimethylaminopurine solution, rational drug level, time of origin and time length, make that triploid times of change rate of Bao is stable and embryo's abnormal rate is low, even under times condition that the change rate is the highest, its relative survival also reaches 90.5%.Preferably water temperature is controlled at 24～25 ℃, the ultimate density of 6-dimethylaminopurine solution is 150 μ mol/l, and the time length is 20min.Because the sterile and low fertility of triploid abalone, the abalone energy i (in vivo) is distributed to change, increase relatively at the energy aspect the somatocyte growth, can accelerate the speed of growth, the improvement of abalone breeding cultures proterties, strengthens its resistance, to improve the output of abalone, have high economic benefit and social benefit.
Embodiment:
Embodiment 1:
A. the 6-dimethylaminopurine (6-DMAP) that U.S. Sigma company is produced is dissolved in distilled water or the dimethyl sulfoxide (DMSO), and is stored in-20～4 ℃ the temperature standby;
B. adopt and catch the kind Bao that the long 8～12cm of shell, no wound, soft body obviously protrude in faucal, plant Bao accelerating total effective temperature and reach more than 1200 ℃, sperm and ovum with ordinary method acquisition abalone obtain zygote with artificial insemination method;
C. zygote being placed temperature is 24～25 ℃ seawater, the test first polar body occurs at 30～50% o'clock, add 6-dimethylaminopurine solution, making the 6-dimethylaminopurine ultimate density in the seawater is 75～150 μ mol/l, time of origin is that first polar body occurs at 30～50% o'clock, time length is 15min, suppresses to take out kind of a Bao after the second polar body;
C. can hasten parturition young hatching with uviolizing seawater method to kind of a Bao.
Embodiment 2:
A. the 6-dimethylaminopurine (6-DMAP) that U.S. Sigma company is produced is dissolved in distilled water or the dimethyl sulfoxide (DMSO), and is stored in-20～4 ℃ the temperature standby;
B. adopt and catch the kind Bao that the long 8～12cm of shell, no wound, soft body obviously protrude in faucal, plant Bao accelerating total effective temperature and reach more than 1200 ℃, sperm and ovum with ordinary method acquisition abalone obtain zygote with artificial insemination method;
C. zygote being placed temperature is 24～25 ℃ seawater, the test first polar body occurs at 30～50% o'clock, add 6-dimethylaminopurine solution, making the 6-dimethylaminopurine ultimate density in the seawater is 75～150 μ mol/l, time of origin is that first polar body occurs at 30～50% o'clock, time length is 20min, suppresses to take out kind of a Bao after the second polar body;
C. can hasten parturition young hatching with uviolizing seawater method to kind of a Bao.
The result of embodiment 1 is as shown in table 1:
| 6-DAMP | Trochophore | The early stage veliger | Later stage veliger | |||||
| (μm) | SR | RSR | AR | RAR | TO | SR | RSR | RSR |
| 75 | 65.5±4.8 | 96.9 | 6.6±6.5 | 103.1 | 30.0 | 76.8±10.3 | 89.3 | 96.8 |
| 100 | 64.1±4.6 | 94.3 | 7.4±7.8 | 115.6 | 46.0 | 74.1±8.4 | 86.1 | 95.3 |
| 125 | 64.1±5.2 | 94.8 | 9.3±5.6 | 145.3 | 47.0 | 73.3±8.3 | 85.2 | 94.8 |
| 150 | 61.2±6.4 | 90.5 | 7.1±6.3 | 110.9 | 54.0 | 66.4±7.3 | 77.2 | 90.5 |
| Contrast | 67.6±4.7 | 100.0 | 6.4±5.1 | 100.0 | 0.0 | 86.0±3.4 | 100.0 | 100.0 |
The result of embodiment 2 is as shown in table 2:
| 6-DMAP | Trochophore | Early stage veliger | Later stage veliger | |||||
| (μM) | SR | RSR | AR | RAR | TO | SR | RSR | RSR |
| 75 | 79.4±4.1 | 95.9 | 14.2±7.9 | 124.6 | 30.1 | 82.7±10.3 | 89.4 | 95.8 |
| 100 | 74.7±4.8 | 90.2 | 18.1±6.1 | 158.8 | 49.0 | 81.4±3.7 | 88.0 | 90.2 |
| 125 | 73.3±7.4 | 88.5 | 19.4±5.1 | 170.2 | 51.2 | 77.5±6.0 | 83.8 | 88.5 |
| 150 | 68.4±7.2 | 82.6 | 19.3±5.0 | 169.3 | 56.0 | 75.2±11.2 | 81.3 | 82.6 |
| Contrast | 82.8±5.2 | 100.0 | 11.4±5.5 | 100.0 | 0.0 | 92.5±7.3 | 100.0 | 100.0 |
Claims (3)
1. the method for an abalone triploid pharmaceutical induction comprises the steps:
A. produce 6-dimethylaminopurine solution;
B. obtain sperm and the ovum of abalone with ordinary method, obtain zygote with artificial insemination method;
C. zygote is placed seawater, suppress the haliotis discus hannai Ino second polar body with 6-dimethylaminopurine solution, the ultimate density of 6-dimethylaminopurine is 75～150 μ mol/l, and time of origin is that first polar body occurs at 30～50% o'clock, and the time length is 15～20min.
2. the method for abalone triploid pharmaceutical induction according to claim 1, it is characterized in that: the solute of the described 6-of producing dimethylaminopurine solution is distilled water or dimethyl sulfoxide (DMSO).
3. the method for abalone triploid pharmaceutical induction according to claim 1, it is characterized in that: described water temperature is 24～25 ℃, and the ultimate density of 6-dimethylaminopurine is 150 μ mol/l, and the described time length is 20min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2005100468370A CN1733908A (en) | 2005-07-05 | 2005-07-05 | Abalone triploid pharmaceutical induction method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2005100468370A CN1733908A (en) | 2005-07-05 | 2005-07-05 | Abalone triploid pharmaceutical induction method |
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| CN1733908A true CN1733908A (en) | 2006-02-15 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101940183A (en) * | 2010-09-26 | 2011-01-12 | 浙江山下湖珍珠集团股份有限公司 | Method for cultivating pearl by inducing triploid hyriopsis cumingii |
| CN107711615A (en) * | 2017-10-31 | 2018-02-23 | 金华职业技术学院 | A kind of method that sterile fish is obtained using immersion fish-egg |
| CN110178799A (en) * | 2019-06-21 | 2019-08-30 | 厦门大学 | A kind of abductive approach of abalone triploid |
| CN110278921A (en) * | 2019-06-21 | 2019-09-27 | 厦门大学 | A kind of method of chemical induction Bao allotriploid |
-
2005
- 2005-07-05 CN CNA2005100468370A patent/CN1733908A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101940183A (en) * | 2010-09-26 | 2011-01-12 | 浙江山下湖珍珠集团股份有限公司 | Method for cultivating pearl by inducing triploid hyriopsis cumingii |
| CN101940183B (en) * | 2010-09-26 | 2012-03-21 | 浙江山下湖珍珠集团股份有限公司 | Method for cultivating pearl by inducing triploid hyriopsis cumingii |
| CN107711615A (en) * | 2017-10-31 | 2018-02-23 | 金华职业技术学院 | A kind of method that sterile fish is obtained using immersion fish-egg |
| CN107711615B (en) * | 2017-10-31 | 2020-08-11 | 金华职业技术学院 | Method for obtaining sterile fish by soaking fish eggs |
| CN110178799A (en) * | 2019-06-21 | 2019-08-30 | 厦门大学 | A kind of abductive approach of abalone triploid |
| CN110278921A (en) * | 2019-06-21 | 2019-09-27 | 厦门大学 | A kind of method of chemical induction Bao allotriploid |
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