CN1709045A - Incubation method for gynogenesis of white crucian - Google Patents
Incubation method for gynogenesis of white crucian Download PDFInfo
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- CN1709045A CN1709045A CNA2005100316880A CN200510031688A CN1709045A CN 1709045 A CN1709045 A CN 1709045A CN A2005100316880 A CNA2005100316880 A CN A2005100316880A CN 200510031688 A CN200510031688 A CN 200510031688A CN 1709045 A CN1709045 A CN 1709045A
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- gynogenesis
- japanese white
- white crucian
- ovum
- crucian
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The present invention relates to gynogenesis technology of fishes. It makes the triangular bream sperm inactivated by UV ray be combined with ovum of Japanese white crusian carp, the activated ovum is undergone the process of cold shock treatment, and the gynogenetic Japanese white crusian carp can be hatched. Said gynogenetic Japanese white crusian carp not only is quick in growth speed, but also its genetic purity and adverse resistance are raised, and its flesh quality also is improved.
Description
Technical field:
The present invention relates to the cultivation of fish, be specifically related to the gynogenesis of fish.
Background technology:
The gynogenesis technology aspects such as breeding, the Sex determination of inquiring into fish and fish monosex cultivation of purifying fish have important effect.Both at home and abroad about the existing many reports of fish gynogenesis research, report is all arranged as the gynogenetic research of carp, grass carp, silver carp, rainbow trout, Tilapia mossambica, red crucian carp, white bass fish.The cultivation of general gynogenesis fishes is the activation of spermatozoa of employing fish monoploid ovum through the genetic material deactivation, handles the dliploid offspring that the genetic material of the main dependence of formation ovum is grown again through chromosomal doubling.Being used for activating the sperm of ovum, generally is the allos sperm, though there be " different smart effect " in the allos sperm, the effect that this " different smart effect " may produce the gynogenesis offspring through inactivation treatment.In fish purification breeding, it is generally acknowledged 1 generation gynogenesis fishes the effect of isozygotying can be equivalent to many effects of purifying for the selfing of fish, therefore in gynogenetic offspring, can obtain the individuality of advantages such as genetic purity height, strong stress resistance, fast growth.Japanese white crucian (Carassius auratus cuvieri) belongs to the fish of Cyprinidae, carp subfamily, it is one of the fastest dliploid crucian (2n=100) of growth rate in the crucian, often be used as one of parent of crossbreeding, but still not ideal enough aspect anti-adversity ability and meat; And megalobrama amblycephala (Megalobrama amblycephala) belongs to the dliploid fish (2n=48) of Cyprinidae, Culter subfamily, compares with Japanese white crucian, belong to different subfamily fish, but it is Fresh ﹠ Tender in Texture.It is good problem to study that the white crucian carp that how to make fast growth obtains improvement in aspect proterties such as genetic purity, anti-adversity ability, meats.
Summary of the invention
The present invention is intended to further improve by the gynogenesis technology of white crucian carp the proterties of white crucian carp.
The present invention is achieved through the following technical solutions the foregoing invention purpose:
The breeding method of gynogenesis Japanese white crucian, will be through the megalobrama amblycephala sperm of ultraviolet inactivation with after the mature egg of Japanese white crucian mixes, through 2～3 minutes the ovum that is activated placed under 0～4 ℃ of temperature condition cold shock to handle 30～40 minutes, then the embryo is hatched under 20～21 ℃ of water temperatures, the fry that hatches is put into the pond and cultures.
Followingly be described in further detail the present invention in conjunction with the accompanying drawings.
Description of drawings
The white crucian carp profile of Fig. 1 gynogenesis diploid
The white crucian carp chromosome of Fig. 2 gynogenesis diploid (2n=100)
The present invention utilizes the ovum of activation of spermatozoa Japanese white crucian of the megalobrama amblycephala of ultraviolet inactivation.Extrude reach sexually matured male megalobrama amblycephala seminal fluid in culture dish, use Hank ' s liquid dilution seminal fluid, the ratio of Hank ' s liquid and seminal fluid is 4: 1, the seminal fluid of dilution distributes with thin layer in culture dish, the culture dish of containing seminal fluid is placed on (rotary speed is 30～40 rev/mins) on the shaking table that is lined with ice bag, and place 2 power to be respectively treatment with irradiation under 15 watts the uviol lamp, and the distance of uviol lamp and seminal fluid is 10～12 centimetres, irradiation time is 30 minutes.Hank ' s formula of liquid is as follows: KCL 0.40g, NaCL 8.00g, NaHCO
30.35g, KH
2PO
40.06g, Na
2HPO
47H
2O 0.09g, Na
2HPO
412H
2O 0.10g, MgSO
47H
2O 0.10g, MgCL
26H
2O 0.10g, CaCL
20.14g, Glucose 1.00g; Adding distil water is assigned to 1 liter.
The seminal fluid that shone mixes with the mature egg of Japanese white crucian, and fully stirs with clean chicken feather, and ovum is tiled to be filled in the clear water culture dish, after 2 minutes, ovum cold shock under 0～4 ℃ of temperature condition is handled 30～40 minutes.The embryo is hatched under 20～21 ℃ of water temperatures then.The embryonic development situation of observation experiment fish, and the record embryonic development is to the situation of the percentage and the incubation rate of primitive gut.The fry that hatches is cultured in the pond.The sexual maturity situation of test experience fish after 1 year.
Detect by the chromosome number of somatic of the direct method of tableting of nephrocyte the experiment fish.The method of the direct method of tableting of nephrocyte is: cultivate the experiment fish after 1～3 day under 18～22 ℃ of water temperatures, the experiment fish is injected PHA 1～3 time, each dosage is 2～8 μ g/ gram body weight, be 12～24 hours blanking time, drew materials preceding 2～6 hours in dissection, injection colchicine, dosage are 2～4 μ g/ gram body weight.Take out nephridial tissue, under physiological saline, shred nephridial tissue, the hypotonic processing of 0.075mol/L KCL, under 20 ℃ of temperature hypotonic 40～60 minutes; Use glacial acetic acid: methyl alcohol (1: 3) is nephrocyte 1～3 time fixedly; On freezing slide, drip sheet; The dyeing of Giemsa dye liquor.
Above-mentioned Japanese white crucian ovum has 80%～85% embryo can grow primitive gut in the gynogenesis process, and 20%～25% embryo can grow demoulding, and the time that ovum is grown embryo's demoulding is 80～100 hours.2 days juvenile fish is put in the pond and cultures behind the demoulding.In shape, the white crucian carp of gynogenesis is similar substantially to common Japanese white crucian, does not have the resemblance (see figure 1) of tangible megalobrama amblycephala.The chromosome testing result shows that the gynogenesis Japanese white crucian is dliploid (2n=100) (see figure 2), and they raised through 1 year, the attainability maturation, and the mature egg of its generation and the fertilization of Japanese white crucian sperm can produce a large amount of offsprings.
The embryo's of Zu Japanese white crucian (♀) * megalobrama amblycephala (♂) fertilization rate is 85%～90% in contrast, and incubation rate is 60%～65%, and the time of hatching demoulding from the embryo that is fertilized is about 78 hours.Chromosome detects and shows that they are triploid (3n=124).In shape, Japanese white crucian (♀) * megalobrama amblycephala (♂) offspring has the feature of Japanese white crucian and megalobrama amblycephala concurrently, as the similar white crucian carp of their health, and the similar megalobrama amblycephala of head.
With a large amount of gynogenesis offspring of surviving that present technique obtains, not only the anti-adversity ability of Japanese white crucian, genetic purity improve, and meat improves, and show that the megalobrama amblycephala sperm is to activate the crucian ovum to carry out gynogenetic ideal material.
Claims (1)
1, a kind of breeding method of gynogenesis Japanese white crucian, it is characterized in that mixing with the mature egg of Japanese white crucian through the megalobrama amblycephala sperm of ultraviolet inactivation, through 2～3 minutes the ovum that is activated placed under 0～4 ℃ of temperature condition cold shock to handle 30～40 minutes, then the embryo is hatched under 20～21 ℃ of water temperatures, the fry that hatches is put into the pond and cultures.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100316880A CN100382684C (en) | 2005-06-10 | 2005-06-10 | Incubation method for gynogenesis of white crucian |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100316880A CN100382684C (en) | 2005-06-10 | 2005-06-10 | Incubation method for gynogenesis of white crucian |
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| CN1709045A true CN1709045A (en) | 2005-12-21 |
| CN100382684C CN100382684C (en) | 2008-04-23 |
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| CNB2005100316880A Active CN100382684C (en) | 2005-06-10 | 2005-06-10 | Incubation method for gynogenesis of white crucian |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100387120C (en) * | 2006-06-08 | 2008-05-14 | 湖南师范大学 | A fish androgenesis method |
| CN102919185A (en) * | 2012-11-27 | 2013-02-13 | 北京市水产科学研究所 | Method for inducing gynogenesis of cyprinus carpio L. by using megalobrama amblycephala sperms |
| CN103493758A (en) * | 2013-09-11 | 2014-01-08 | 海南大学 | Method for artificially inducting gynogenesis of grouper to generate fry |
| CN104904638A (en) * | 2015-06-29 | 2015-09-16 | 中国科学院水生生物研究所 | A cultivation method for carassius auratus gynogenesis fries |
| CN107318719A (en) * | 2017-08-16 | 2017-11-07 | 湖南师范大学 | The method of fancy carp sperm induction grass carp gynogenesis and the application of gynogenetic grass carp |
| CN109329122A (en) * | 2018-10-11 | 2019-02-15 | 湖南师范大学 | It is a kind of improve Japanese white crucian selection and its strain method for building up |
| CN109329128A (en) * | 2018-10-31 | 2019-02-15 | 湖南师范大学 | Method for building up and conjunction side crucian carp No. 2 numbers breeding methods of Japanese white crucian and red crucian carp crosses |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102007879B (en) * | 2010-11-16 | 2012-11-14 | 湖南师范大学 | Method for cultivating gynogenetic carps |
| CN110150185B (en) * | 2019-05-28 | 2021-08-10 | 中国三峡建设管理有限公司 | Large-scale cultivation method for megalobrama amblycephala fries |
-
2005
- 2005-06-10 CN CNB2005100316880A patent/CN100382684C/en active Active
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100387120C (en) * | 2006-06-08 | 2008-05-14 | 湖南师范大学 | A fish androgenesis method |
| CN102919185A (en) * | 2012-11-27 | 2013-02-13 | 北京市水产科学研究所 | Method for inducing gynogenesis of cyprinus carpio L. by using megalobrama amblycephala sperms |
| CN103493758A (en) * | 2013-09-11 | 2014-01-08 | 海南大学 | Method for artificially inducting gynogenesis of grouper to generate fry |
| CN104904638A (en) * | 2015-06-29 | 2015-09-16 | 中国科学院水生生物研究所 | A cultivation method for carassius auratus gynogenesis fries |
| CN107318719A (en) * | 2017-08-16 | 2017-11-07 | 湖南师范大学 | The method of fancy carp sperm induction grass carp gynogenesis and the application of gynogenetic grass carp |
| CN107318719B (en) * | 2017-08-16 | 2020-05-01 | 湖南师范大学 | Method for inducing gynogenesis of grass carp by aid of koi sperms and application of gynogenesis grass carp |
| CN109329122A (en) * | 2018-10-11 | 2019-02-15 | 湖南师范大学 | It is a kind of improve Japanese white crucian selection and its strain method for building up |
| CN109329122B (en) * | 2018-10-11 | 2021-05-11 | 湖南师范大学 | Breeding method of improved Japanese white crucian carp and establishment method of strain thereof |
| CN109329128A (en) * | 2018-10-31 | 2019-02-15 | 湖南师范大学 | Method for building up and conjunction side crucian carp No. 2 numbers breeding methods of Japanese white crucian and red crucian carp crosses |
| CN109329128B (en) * | 2018-10-31 | 2022-05-17 | 湖南师范大学 | Method for establishing Japanese white crucian carp and red crucian carp hybrid strain and method for cultivating cross crucian carp No. 2 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100382684C (en) | 2008-04-23 |
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Assignee: Hunan Xiangyun Biological Co., Ltd. Assignor: Hunan Normal University Contract record no.: 2010430000180 Denomination of invention: Incubation method for gynogenesis of white crucian Granted publication date: 20080423 License type: Exclusive License Open date: 20051221 Record date: 20101206 |