CN100382685C - Red crucian, Japanese white crucian and megalobrame amblycephala subfamily distant hydridization method - Google Patents
Red crucian, Japanese white crucian and megalobrame amblycephala subfamily distant hydridization method Download PDFInfo
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- CN100382685C CN100382685C CNB2005100316895A CN200510031689A CN100382685C CN 100382685 C CN100382685 C CN 100382685C CN B2005100316895 A CNB2005100316895 A CN B2005100316895A CN 200510031689 A CN200510031689 A CN 200510031689A CN 100382685 C CN100382685 C CN 100382685C
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The present invention relates to a distant hybridization technique of fish. The present invention selects a red crusian carp and a Japanese white crusian carp as a female parent, and uses a megalobrama amblycephala as a male parent; an artificial dry method is adopted to make insemination, and the fertilized ovum is incubated so that the filial generation of triploid and tetraploid is bred. The present invention not only can improve characters, but also is a new way for producing the fish of triploid and tetraploid.
Description
Technical field:
The present invention relates to the cross method of fish, be specifically related to the distant hybridization technology of fish.
Background technology:
Hybridization technique is an improvement fish proterties method commonly used, general affiliation between planting or the hybridization between planting be distant hybridization.Both at home and abroad about the more existing reports of the research of fish distant hybridization, as flathead (♀) * silver carp (♂), grass carp (♀) * silver carp (♂), red crucian carp (♀) * carp (♂) etc., wherein preceding two kinds is hybridization between subfamily.By distant hybridization, the proterties of fish can obtain improvement, the filial generation that forms as the inter-genera distant hybridization of red crucian carp and Xiangjiang wild carps have fast growth, Fresh ﹠ Tender in Texture, premunition by force, the survival rate advantages of higher.Red crucian carp (Carassius auratus red var.) belongs to the liploid fish (2n=100) in Cyprinidae, the carp subfamily, Japanese white crucian (Carassius auratus cuvieri) belongs to the liploid fish (2n=100) in Cyprinidae, the carp subfamily, and megalobrama amblycephala (Megalobrama amblycephala) belongs to the liploid fish (2n=48) in Cyprinidae, the Culter subfamily; Aspect biological propertyes such as heredity, classification and the bodily form, there is bigger difference between red crucian carp, Japanese white crucian and the megalobrama amblycephala, especially chromosome number of somatic is widely different between them, the chromosome number of red crucian carp and Japanese white crucian is 100, and the chromosome number of megalobrama amblycephala is 48, and not sexual intercourse at double, so the distant hybridization difficulty is more.So far the report that does not also have the distant hybridization success between crucian and megalobrama amblycephala subfamily both at home and abroad.Explore a kind of technology that hybridization between red crucian carp, Japanese white crucian and the megalobrama amblycephala is succeedd and have using value.
Summary of the invention:
The present invention is intended to obtain by father and mother's selection originally the success of crucian and megalobrama amblycephala distant hybridization, and the good offspring's individuality of acquired character.
The present invention seeks to be achieved through the following technical solutions:
With red crucian carp or Japanese white crucian is female parent, is male parent with the megalobrama amblycephala, and the ovum of sexually matured red crucian carp or Japanese white crucian is mixed with the sperm of megalobrama amblycephala respectively, carries out artificial dry method insemination, and fertilized egg is hatched under 20～21 ℃ of water temperatures, and fry is raised in the pond.
Below in conjunction with further the present invention in detail of accompanying drawing.
Description of drawings:
The red crucian carp of Fig. 1 (♀) * megalobrama amblycephala (♂) offspring profile
The chromosome (4n=148) of tetraploid fish among the red crucian carp of Fig. 2 (♀) * megalobrama amblycephala (♂) offspring
The white crucian carp of Fig. 3 (♀) * megalobrama amblycephala (♂) offspring profile
The chromosome (3n=124) of triploid fish among the white crucian carp of Fig. 4 (♀) * megalobrama amblycephala (♂) offspring
Embodiment:
Key of the present invention is father and mother's selection originally, with red crucian carp, Japanese white crucian is female parent, with the megalobrama amblycephala is male parent, concrete grammar is: select sexually matured female red crucian carp, white crucian carp, extrude their mature egg, sperm with megalobrama amblycephala is mixed into the insemination of pedestrian worker's dry method respectively, fully stirs sperm and ovum with clean chicken feather.Fertilized egg is placed in the culture dish hatches, 20～21 ℃ of water temperatures, and the fry that hatches is raised in the pond.Detect with the chromosome number of somatic of the direct method of tableting of nephrocyte the offspring of red crucian carp (♀) * megalobrama amblycephala (♂), white crucian carp (♀) * megalobrama amblycephala (♂).The method of the direct method of tableting of nephrocyte is: cultivate the experiment fish after 1～3 day under 18～22 ℃ of water temperatures, the experiment fish is injected PHA 1～3 time, each dosage is 2～8 μ g/ gram body weight, be 12～24 hours blanking time, drew materials preceding 2～6 hours in dissection, injection colchicine, dosage are 2～4 μ g/ gram body weight.Take out nephridial tissue, under physiological saline, shred nephridial tissue, the hypotonic processing of 0.075mol/L KCL, under 20 ℃ of temperature hypotonic 40～60 minutes; Use glacial acetic acid: methyl alcohol (1: 3) is nephrocyte 1～3 time fixedly; On freezing slide, drip sheet; The dyeing of Giemsa dye liquor.
Experimental results show that, in red crucian carp (♀) * megalobrama amblycephala (♂), Japanese white crucian (♀) * megalobrama amblycephala (♂) combination, can both produce the offspring of survival, and their reciprocal cross combinations red crucian carp (♂) * megalobrama amblycephala (♀), Japanese white crucian (♂) * megalobrama amblycephala (♀) combination can not produce the offspring of survival.The fertilization rate of red crucian carp (♀) * megalobrama amblycephala (♂) is 80%～85%, and incubation rate reaches 22%～30%.Two kinds of body colours appear among red crucian carp (♀) * megalobrama amblycephala (♂) offspring, a kind of body colour is darker, approximate ink black, another kind of body colour is a cinerous, and on the bodily form, these filial generations have the feature of red crucian carp and megalobrama amblycephala concurrently, head as them is partial to megalobrama amblycephala, and body part is similar to red crucian carp, but can significantly find out the feature of filial generation, obviously increases (see figure 1) as height.In red crucian carp (♀) * megalobrama amblycephala (♂) offspring, find to have tetraploid (4n=148) (see figure 2), also find to have triploid (3n=124).The fertilization rate of Japanese white crucian (♀) * megalobrama amblycephala (♂) is 85%～90%, and incubation rate is 60%～65%.The body colour of Japanese white crucian (♀) * megalobrama amblycephala (♂) is a silver gray, and on the bodily form, they have the feature of Japanese white crucian and megalobrama amblycephala concurrently, be similar to megalobrama amblycephala as their head, and health is partial to the Japanese white crucian (see figure 3).Detect Japanese white crucian (♀) * megalobrama amblycephala (♂) offspring's chromosome, prove that they are triploid (3n=124) (see figure 4)s.
This method is for utilizing the fish distant hybridization and produce novel tetraploid and triploid fish having been opened up new way.By further seed selection, can select the novel tetraploid and the triploid fish of collection red crucian carp and megalobrama amblycephala advantage, and market day the raw white crucian carp and the megalobrama amblycephala advantage novel triploid fish.
Claims (1)
1. the inter-subfamily distant hybridization method of a red crucian carp, Japanese white crucian and megalobrama amblycephala, it is characterized in that with red crucian carp or Japanese white crucian be female parent, with the megalobrama amblycephala is male parent, the ovum of sexually matured red crucian carp or Japanese white crucian is carried out artificial dry method insemination with the sperm of megalobrama amblycephala respectively, fertilized egg is hatched under 20～21 ℃ of water temperatures, and fry is raised in the pond.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104585091A (en) * | 2015-01-12 | 2015-05-06 | 湖南师范大学 | Subfamily distant hybridization for German mirror carp and megalobrama amblycephala and application of tetraploid hybrid fishes |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101341859B (en) * | 2008-08-29 | 2010-12-01 | 湖南师范大学 | Distant hybridization method for megalobrama amblycephala and yellow catfish |
| CN101884314B (en) * | 2010-07-26 | 2011-11-23 | 湖南师范大学 | Method for inter-subfamily distant hybridization of grass carp and megalobrama amblycephala |
| CN102090360B (en) * | 2010-12-17 | 2012-02-29 | 中国水产科学研究院黄海水产研究所 | High-efficient induction method of tetraploid cynoglossus semilaevis fish fries |
| CN103091144A (en) * | 2013-01-29 | 2013-05-08 | 中国水产科学研究院黑龙江水产研究所 | Method for preparing chromosomes through large-scale barbel fish nephrocyte in-vivo culture |
| CN110199918B (en) * | 2019-06-14 | 2021-07-09 | 湖南师范大学 | Method for establishing autotetraploid carp strain and method for breeding autotetraploid carp |
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2005
- 2005-06-10 CN CNB2005100316895A patent/CN100382685C/en active Active
Non-Patent Citations (3)
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| 杂交鲫（白鲫♀×散鳞镜鲤）及其双亲染色体组型的研究. 张克俭，高健，张景龙，何玉明，王维善.水产学报，第19卷第4期. 1995 * |
| 红鲫(♀)×湘江野鲤(♂)F_2和F_3的染色体研究. 冯浩，刘少军，张轩杰，罗琛，周工健，姚占洲，刘筠.中国水产科学，第8卷第2期. 2001 * |
| 鲫、鲤杂交制种技术的研究. 徐彦山，唐虹，刘英.大连水产学院学报，第18卷第1期. 2003 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104585091A (en) * | 2015-01-12 | 2015-05-06 | 湖南师范大学 | Subfamily distant hybridization for German mirror carp and megalobrama amblycephala and application of tetraploid hybrid fishes |
| CN104585091B (en) * | 2015-01-12 | 2017-02-22 | 湖南师范大学 | Subfamily distant hybridization for German mirror carp and megalobrama amblycephala and application of tetraploid hybrid fishes |
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