CN1698415A - Artificial domestication and planting method for wild binwang mushroom - Google Patents
Artificial domestication and planting method for wild binwang mushroom Download PDFInfo
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- CN1698415A CN1698415A CN 200410100497 CN200410100497A CN1698415A CN 1698415 A CN1698415 A CN 1698415A CN 200410100497 CN200410100497 CN 200410100497 CN 200410100497 A CN200410100497 A CN 200410100497A CN 1698415 A CN1698415 A CN 1698415A
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Abstract
The invention provides an artificial domestication and planting method for wild binwang mushroom, wherein the bacterial cultivating process comprises, (1) purifying the mycelium of spore germination in the field, or incising fruiting body of the fresh mushroom under the asepsis condition, culturing a hyphostroma on the culture medium, culturing the mother bacterial again, making original breed after generation addition breeding, finally conducting the routine culture of the original breed based on the culture medium. The mushroom manual cultivating method comprises the steps of culturing on purpose-made cultivation material, mixing the culture material with turfy soil, sterilizing and culturing the mushroom in culture chambers with the temperature of 22+-2 deg. C.
Description
Technical field:
The present invention is a kind of wild edible fungus domesticating cultivation method, is specifically related to a kind of breeding strain and artificial cultivation method of wild binwang mushroom; Binwang mushroom is to belong to Agaricales Agaricales, the white Tricholomataceae of mushroom section, and the dark brown that belongs to Lyophyllum from the pleat umbrella is from the pleat umbrella.
Background technology:
Wild binwang mushroom, formal name used at school dark brown grow in the Zhangwu County, Liaoning of 121 °-122 ° of east longitudes, 42 °-43 ° of north latitude, the two sides of resting and take nourishing food to build up one's health recuperate and herd the river in second largest river, red river, source from the pleat umbrella.Along the river the two sides turfy soil is continuous 10 kilometers, and is wide 1 kilometer, dark 6 meters.Along the river both sides are spill valley shape, and the outside, valley is the rolling drift sand, and not even a blade of grass grows, and are moist in the valley, and ground temperature, temperature are fit to crop growth.Be that binwang mushroom is contained the phase of sending out annual late August to mid-October.Can see in the corn field and in the peat composed of rotten mosses ground fissure by the river in the thick grass.
Binwang mushroom is grown thickly more, and single living less, young mushroom is a brown, becomes the brown white of mushroom, and the many places of thing of shading are creamy white, spore print white, also visible fairy ring.Bright product, dry product all can be smelt medicine fragrance.
Before more than 300 year, Zhangwu County, Liaoning and Inner Mongol boundary are that the Qing Dynasty and Mongolian two countries have a common boundary, and the emperor of Qing Dynasty Tai Ji has a prefecture king to claim many Luo Bintu prefecture king period, guards the border area at this, and every year, autumn, chieftain just contributed the emperor with this game, and so this mushroom is the binwang mushroom of gaining the name.
The detection of binwang mushroom is identified:
1) binwang mushroom sporophore shape:
The flat hemispherical of cap is to open and flat, and the middle part is protruding slightly or open and flat or recessed slightly, and the surface is that dull gray is white or smoky gray is extremely white, level and smooth slightly.The edge is open and flat to digging, and does not have obvious striped.Bacterial context white does not have obvious metachromasia, the quality delicacy to dirty white.The lamella initial stage directly gives birth to, bends and give birth to prolonging life, and is close slightly, and white is to dirty white, not isometric.Stem is closely cylindrical, and is crooked slightly, the initial stage taupe, and white, long 3cm-8cm, thick 0.4cm-0.6cm, base portion is closely grown thickly, and how to grow together on the mycelia piece and adheres to thin sand and soil.
2) lamella is organized microscopy:
Get the lamella fritter and do sheet, drip KOH liquid, place under the high-power microscope and observe, spore is subsphaeroidal to wide ellipse, and is smooth, colourless.Drip Melzer liquid, non-paste reaction, 5 μ m-7 μ m, load tool 4 stigmas.
3) send evaluation sample sample, detect and name first through Institute of Microorganism, Academia Sinica, identification is identified and the spore chemical reaction through morphology, belong to Agaricales Agaricales, the white Tricholomataceae of mushroom section, the dark brown that belongs to Lyophyllum from the pleat umbrella is from the pleat umbrella, and local name claims binwang mushroom, guest youth mushroom again, and the Latin formal name used at school is: Lyophyllum fumosum (Pers.:Fr.) Kummer.Document record and all edible delicious U.S. in locality.
Centuries, people are predatory in addition plucked owing to farmland improvement, turfy soil exploitation, chemical fertilizer, the applications of pesticide, and this mushroom has been on the brink of extinction.Price has reached per kilogram hundreds of unit.But still there is not tame report at present about binwang mushroom.
Summary of the invention:
The present invention just provides a kind of breeding method and artificial cultivation method of wild binwang mushroom strains.
The present invention adopts following technical scheme:
The breeding method of bacterial classification:
1, utilize the mycelium purifying of open-air spore germination of catching after, or under aseptic condition, cut fresh binwang mushroom fruit body, get a ferfas meat tissue, put into to fry in shallow oil on the clarified solution preparation PDA medium of juice after 20 minutes and cultivate with turfy soil; In temperature is 6 ℃-30 ℃ (optimal temperature is 18 ℃-25 ℃), and optimum pH value is 6-7, unglazed cultivation, and mycelia is a white, promptly is covered with mycelia after 24-30 days and is female the kind on media surface;
2, the rejuvenation of female kind is cultivated, and makes original seed behind the adding generation;
3, original seed carries out routine on the medium for 30 parts-40 parts of turfy soils, 35 parts-40 parts of corncobs (or cotton seed hulls), 1 part-2 parts of limes, 15 parts-20 parts in wheat bran (or rice chaff), 3 parts-5 parts of corn flours, 1 part-2 parts in gypsum at prescription and produces to cultivate to produce and plant.
The binwang mushroom artificial cultivation method:
1. the prescription of cultivate material is by weight: 40 parts-85 parts of turfy soils, 20 parts-40 parts of corncobs, 10 parts-20 parts of corn stalks (or straw), 10 parts-15 parts in wheat bran, 2 parts-4 parts of corn flours, 1 part-2 parts of limes, 1 part-2 parts in gypsum, 0.1 part-0.2 part of mushroom bodyguard.
2. after the composts or fertilisers of cultivating that will be mixed with turfy soil is mixed thoroughly, convert water to water content 55%-65%.
3. sterilization.
4. binwang mushroom production kind is broadcast in the composts or fertilisers of cultivating under aseptic condition, placed 22 ℃ ± 2 ℃ culturing room to cultivate.Preservation date: on December 28th, 2004; Deposit number: CGMCC NO.1283;
The classification name: dark brown is from gill fungus Lyophyllum fumosum
Depositary institution: China Committee for Culture Collection of Microorganisms common micro-organisms center
Address: No. 13, North No.1 Row, Zhongguancun, Haidian District, Beijing City Institute of Microorganism, Academia Sinica
Embodiment:
Producing kind of a medium is:
Embodiment 1:
40 parts of corncobs, 40 parts of turfy soils, 15 parts in wheat bran, 1 part in gypsum, 3 parts of corn flours, 1 part of lime.
Embodiment 2:
40 parts of cotton seed hullss, 40 parts of turfy soils, 15 parts in wheat bran, 1 part in gypsum, 3 parts of corn flours, 1 part of lime.
Embodiment 3:
35 parts of corncobs, 40 parts of turfy soils, 20 parts of rice chaff, 3 parts of corn flours, 1 part of lime, 1 part in gypsum.
Plant formulation:
Embodiment 4:
75 parts of turfy soils, 5 parts of corncobs, 5 parts of corn stalks, 10 parts in wheat bran, 3 parts of corn flours, 1 part of lime, 1 part in gypsum, 0.1 part of mushroom bodyguard.
Embodiment 5:
45 parts of turfy soils, 20 parts of corn stalks, 20 parts of corncobs, 10 parts in wheat bran, 3 parts of corn flours, 1 part of lime, 1 part in gypsum, 0.1 part of mushroom bodyguard.
Embodiment 6:
42 parts of turfy soils, 20 parts of corncobs, 20 parts of straw, 13 parts in wheat bran, 3 parts of corn flours, 1 part of lime, 1 part in gypsum, 0.1 part of mushroom bodyguard.
The three-dimensional pocket type cultivation of booth wall formula:
After the composts or fertilisers of cultivating that is mixed with turfy soil mixed thoroughly, convert water to water content 60%-70%, composts or fertilisers of cultivating is packed in 17cm * 35cm (or other specification) bag, degree of tightness is moderate, the tightening belt mouth places sterilization in the normal-pressure sterilization pot, sterilization time is after kettle temperature reaches 100 ℃ on the atmosphere, to pick up counting 15-17 hour.
When treating that the bag cooling is below 25 ℃, will produce to plant and under aseptic condition, broadcast in the culture bag.The both sides inoculation places 22 ℃ ± 2 ℃ to cultivate indoor cultivation with connecting the culture bag of planting, and keeps room air fresh, suitable light scattering, and relative air humidity 60%-70%, mycelia was covered with in about 60 days.
Double crouch of the bacterium bag that covers with mycelia put, put to 5-8 high, separate with the 2cm turfy soil between every layer of bag.Carry out wall formula fruiting.
The cultivation of binwang mushroom booth ridge-up bed
1) groundbed is manufactured: horizontal booth, and 1.0 meters of bedsides, dark 0.1 meter, bed is long to adapt with the booth width.Stay 0.4 meter passageway between bed and the bed.The turfy soil that adds at the bottom of the 1% whitewash solution-treated bed and covered with 0.1% carbendazim solution or 0.1% chlorophos solution.
2) the booth space disinfection is handled: every cubic metre with stifling the getting final product of Cosan 10 grams.
3) the bacterium bag that will cover with mycelia moves in the cultivation canopy, and crouching is put in the geosyncline, is covered with the 3cm-5cm turfy soil after filling and leading up the gap with turfy soil, spreads one deck 0.5cm fine sand on the turfy soil.About 15 days visible damp mushrooms.
Plant (with the gramineous crop interplanting) binwang mushroom
1) place is selected: the place should be selected in the well-ventilated, away from the abundant plot of the turfy soil content of pollution source.
2) cold bed of cultivation binwang mushroom is located between the contour stalk crop row of grass family, general wide 20cm-30cm, and dark 10cm-12cm, length is not limit.General ridge-up bed should be east-west, makees bed early spring, and pavement 40cm-60cm between bed uses as management.Convex is accomplished at the bed end, and sowing is preceding with lime sterilization (200 gram/m
2), spraying insecticide insect protected (chlorophos 10 gram/m
2).
3) the bacterium rod that will send out bacterium good crouched at the beginning of annual 7 months and is put in the ready-made cold bed, put along bed, stayed the space of 0.5cm between bag and bag, filled and led up with turfy soil, covered about the native 5cm of nature on then.The bacterium rod that maybe will send out bacterium good crouched in pairs at the beginning of annual 7 months and is put between the gramineous crop strain, dark 10cm place, on cover the native 5cm of nature.
4) it is moistening to keep bed to go up, but about 30-40 days fruitings.
Claims (8)
1, wild binwang mushroom domestication and cultivation method thereof, the breeding method of bacterial classification: it is characterized in that:
(1), utilize the mycelium purifying of open-air spore germination of catching after, or under aseptic condition, cut fresh binwang mushroom fruit body, get a ferfas meat tissue, put into to fry in shallow oil on the clarified solution preparation PDA medium of juice after 20 minutes and cultivate with turfy soil; In temperature is 6 ℃-30 ℃ (optimal temperature is 18 ℃-25 ℃), and optimum pH value is 6-7, unglazed cultivation, and mycelia is a white, promptly is covered with mycelia after 24-30 days and is female the kind on media surface;
(2), female plant rejuvenation and cultivate, make original seed behind the adding generation;
(3), original seed carries out routine on the medium for 30 parts-40 parts of turfy soils, 35 parts-40 parts of corncobs (or cotton seed hulls), 1 part-2 parts of limes, 15 parts-20 parts in wheat bran (or rice chaff), 3 parts-5 parts of corn flours, 1 part-2 parts in gypsum at prescription and produces to cultivate to produce and plant.
2, wild binwang mushroom domestication and cultivation method thereof, binwang mushroom artificial cultivation method: it is characterized in that:
(1), the prescription of cultivate material is by weight: 40 parts-85 parts of turfy soils, 20 parts-40 parts of corncobs, 10 parts-20 parts of corn stalks (or straw), 10 parts-15 parts in wheat bran, 2 parts-4 parts of corn flours, 1 part-2 parts of limes, 1 part-2 parts in gypsum, 0.1 part-0.2 part of mushroom bodyguard;
(2), after the composts or fertilisers of cultivating that will be mixed with turfy soil mixes thoroughly, convert water to water content 55%-65%;
(3), sterilization;
(4), binwang mushroom production kind is broadcast in the composts or fertilisers of cultivating under aseptic condition, place 22 ℃ ± 2 ℃ culturing room to cultivate.
3, wild binwang mushroom domestication according to claim 1 and cultivation method thereof is characterized in that 40 parts of corncobs, 40 parts of turfy soils, 15 parts in wheat bran, 1 part in gypsum, 3 parts of corn flours, 1 part of lime.
4, wild binwang mushroom domestication according to claim 1 and cultivation method thereof is characterized in that 40 parts of cotton seed hullss, 40 parts of turfy soils, 15 parts in wheat bran, 1 part in gypsum, 3 parts of corn flours, 1 part of lime.
5, wild binwang mushroom domestication according to claim 1 and cultivation method thereof is characterized in that 35 parts of corncobs, 40 parts of turfy soils, 20 parts of rice chaff, 3 parts of corn flours, 1 part of lime, 1 part in gypsum.
6, wild binwang mushroom domestication according to claim 2 and cultivation method thereof is characterized in that 75 parts of turfy soils, 5 parts of corncobs, 5 parts of corn stalks, 10 parts in wheat bran, 3 parts of corn flours, 1 part of lime, 1 part in gypsum, 0.1 part of mushroom bodyguard.
7, wild binwang mushroom domestication according to claim 2 and cultivation method thereof is characterized in that 45 parts of turfy soils, 20 parts of corn stalks, 20 parts of corncobs, 10 parts in wheat bran, 3 parts of corn flours, 1 part of lime, 1 part in gypsum, 0.1 part of mushroom bodyguard.
8, wild binwang mushroom domestication according to claim 2 and cultivation method thereof is characterized in that 42 parts of turfy soils, 20 parts of corncobs, 20 parts of straw, 13 parts in wheat bran, 3 parts of corn flours, 1 part of lime, 1 part in gypsum, 0.1 part of mushroom bodyguard.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103070007A (en) * | 2011-10-25 | 2013-05-01 | 何寒 | Method for breeding and purifying edible mushroom strains at test tube medium-less and medium critical points |
| CN104521560A (en) * | 2014-12-12 | 2015-04-22 | 辽宁省农业科学院 | Lyophyllum fumosurn molecular identification and strain production method |
| CN104584866A (en) * | 2015-02-05 | 2015-05-06 | 莆田市农业科学研究所 | Off-season cultivation method of wild edible fungus |
| CN104987156A (en) * | 2015-07-09 | 2015-10-21 | 辽宁省农业科学院蔬菜研究所 | Lyophyllum fumosurn culture medium using fermented bran and method for cultivating lyophyllum fumosurn |
| CN107125028A (en) * | 2017-06-05 | 2017-09-05 | 吉林省生物研究所 | A kind of wild yellow ring squama agaric domestication and artificial culturing method |
| CN108617401A (en) * | 2018-04-23 | 2018-10-09 | 大兴安岭地区农业林业科学研究院（大兴安岭林业集团公司农业林业科学研究院） | A kind of cultivation of wild high-quality green tea mushroom strain and cultural method |
| CN108718911A (en) * | 2018-06-07 | 2018-11-02 | 哈密伊吾马场 | A kind of artificial domesticating cultivation method of the wild orange mushroom in Tianshan Mountains |
Family Cites Families (3)
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| CN1040905A (en) * | 1988-09-07 | 1990-04-04 | 董天敏 | A kind of artificial dry-fungus cultivation method |
| CN1073339C (en) * | 1997-12-31 | 2001-10-24 | 中国科学院新疆生物土壤沙漠研究所 | High-yield awei mushroom cultivation method |
| CN1457631A (en) * | 2003-03-07 | 2003-11-26 | 关弦敏 | Cultivating technology for brown mushroom |
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- 2004-12-29 CN CNB2004101004970A patent/CN1326446C/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103070007A (en) * | 2011-10-25 | 2013-05-01 | 何寒 | Method for breeding and purifying edible mushroom strains at test tube medium-less and medium critical points |
| CN104521560A (en) * | 2014-12-12 | 2015-04-22 | 辽宁省农业科学院 | Lyophyllum fumosurn molecular identification and strain production method |
| CN104584866A (en) * | 2015-02-05 | 2015-05-06 | 莆田市农业科学研究所 | Off-season cultivation method of wild edible fungus |
| CN104987156A (en) * | 2015-07-09 | 2015-10-21 | 辽宁省农业科学院蔬菜研究所 | Lyophyllum fumosurn culture medium using fermented bran and method for cultivating lyophyllum fumosurn |
| CN104987156B (en) * | 2015-07-09 | 2018-05-22 | 辽宁省农业科学院蔬菜研究所 | A kind of method of binwang mushroom culture medium and cultivation binwang mushroom using mushroom bran |
| CN107125028A (en) * | 2017-06-05 | 2017-09-05 | 吉林省生物研究所 | A kind of wild yellow ring squama agaric domestication and artificial culturing method |
| CN108617401A (en) * | 2018-04-23 | 2018-10-09 | 大兴安岭地区农业林业科学研究院（大兴安岭林业集团公司农业林业科学研究院） | A kind of cultivation of wild high-quality green tea mushroom strain and cultural method |
| CN108617401B (en) * | 2018-04-23 | 2020-02-07 | 大兴安岭地区农业林业科学研究院（大兴安岭林业集团公司农业林业科学研究院） | Cultivation and cultivation method of wild tricholoma matsutake strains |
| CN108718911A (en) * | 2018-06-07 | 2018-11-02 | 哈密伊吾马场 | A kind of artificial domesticating cultivation method of the wild orange mushroom in Tianshan Mountains |
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