CN1685796A

CN1685796A - Method of raising cordyceps production stability

Info

Publication number: CN1685796A
Application number: CN 200510034376
Authority: CN
Inventors: 郑贵朝; 胡事君
Original assignee: DONGGUAN CITY INST OF BIOTECHNOLOGY
Current assignee: DONGGUAN CITY INST OF BIOTECHNOLOGY
Priority date: 2005-04-29
Filing date: 2005-04-29
Publication date: 2005-10-26
Anticipated expiration: 2025-04-29
Also published as: CN100348713C

Abstract

A method for increasing the production stability of cordyceps includes preculturing in the culture liquid, inoculating from a test tube to 5-8 bottles of suspension, further inoculating from one bottle to 100 bottles, and secondary preculturing. Its advantages are short period and high quality stability.

Description

Improve the method for cordyceps production stability

Technical field

The present invention relates to a kind of method that improves cordyceps production stability.

Background technology

The worm summer in winter, worm was a kind of extremely precious medical, edible fungi, in recent years, along with improving constantly of people's living standard, health care the reach of science and to the continuous expansion in Chinese caterpillar fungus understanding field, the medicine, health food, beverage, the cosmetic development that with the Chinese caterpillar fungus are primary raw material emerge in an endless stream, not only the domestic market enlarges day by day to the demand of grass-and-insect painting, and the demand of international markets such as Southeast Asia, Japan, the U.S. is also in continuous increase.Because the wild Chinese caterpillar fungus resource is very rare, excavate difficultly, usually occur that supply falls short of demand, price rises steadily, soaring, made the multiplication of Chinese caterpillar fungus price, and immoderate excavating both caused the exhaustion that is close to of wild resource, caused the destruction to natural ecological environment again.Therefore, Cordyceps militaris has been subjected to extensive concern both domestic and external as unique substitute that can replace wild worm summer in winter worm.

At present, the artificial culture technology of fruiting bodies of cordyceps militaris is succeedd, the market development promise well, but fruiting bodies of cordyceps militaris is cultivated complex procedures, realizes the restraining factors that also exist some can not be ignored in scale and the industrialization production process.General method of cultivating Cordyceps militaris is: select excellent species for use, scrape the mycelium of getting chamfered surface, be inoculated in the liquid nutrient medium, suspension culture obtained liquid spawn about 4 days.Liquid-spawn inoculation to rice medium, is alternately cultivated through illumination-dark, gathered after the maturation.General cultural method, a test tube strains can only be inoculated 5-8 bottle liquid spawn.And general cultural method is not taken a sample and is planted experimentally, and has both made the increase sampling plant experimentally link, can only take a sample on test tube yet, and effect is relatively poor.General cultural method, Cordyceps militaris spawn on rice medium, the early stage growth be nourish and grow and reproductive growth carry out simultaneously, mycelia needs just can cover with rice medium about 10-15 days, and in the same culture vessel, the growth of fruit body has early to be had late, adds the competition of growing space and nutrition, must cause when gathering some old excessively, some is too tender, be difficult to hold collecting time, the more sturdy hypertrophy of the fruit body that grows earlier, after the fruit body that grows more very thin small and weak.Influence outward appearance and the quality of Cordyceps militaris.

Summary of the invention:

The purpose of this invention is to provide a kind of output and quality stability that can guarantee Cordyceps militaris, can shorten the method for the raising cordyceps production stability of growth cycle again.The present invention introduces secondary and cultivates in advance, cultivates in advance for the first time and carries out in liquid.Because suspension has very strong homogeneity, therefrom sampling has better representativeness.Sampling is planted experimentally, and can further confirm the productivity of bacterial classification, guarantees the stability of producing.Pre-for the second time the cultivation carried out in the dark condition of pre-incubation chamber, when cordyceps species is cultivated in dark surrounds, only to nourish and grow, 3-4 days time can be covered with medium, transfer to subsequently in the culturing room after the continuous illumination 3 days, mycelium changes reproductive growth simultaneously over to, thus the cordyceps militaris sporocarp synchronous growth in the culture vessel, the maturity unanimity, thickness, length homogeneous in appearance, can timely collecting, both shortened the incubation growth cycle of Chinese caterpillar fungus, guarantee the stability of product quality again.

The objective of the invention is to realize: select excellent species (test tube kind) for use by following approach, introduce the pre-stability of producing of cultivating of secondary with the raising Cordyceps militaris, concrete steps are: a, scrape the mycelium of getting chamfered surface, be inoculated in the liquid nutrient medium, suspension culture 2～3 days, introduce pre-for the first time the cultivation, when cultivating in advance, 1 test tube strains can be inoculated 5-8 bottle suspension.B, suspension sampled plant experimentally, all the other are put in the 3-5 ℃ of refrigerator and preserve.Must put into refrigerator after the sampling, the time of preserving in refrigerator preferably is no more than 60 days at once.After c, the affirmation bacterial classification productivity, the taking-up sampling is planted experimentally the suspension of acting normally and is inoculated, and eliminates other suspension.Get suspension 1.5-2ml during inoculation and be inoculated in the liquid nutrient medium, suspension culture 4 days obtains liquid spawn.Every bottle of switchable 100 bottles of liquid spawns of suspension.D, with liquid-spawn inoculation to rice medium, under the dark condition of pre-incubation chamber, carry out the second time and pre-cultivate dark culturing 3-4 days.E, transfer in the culturing room continuous illumination 3 days, make daylight every day afterwards, the alternately cultivation of dark each illumination-dark of 12 hours in evening, incubation time 40-45 days.According to routine, culturing room's temperature is 18-20 ℃, and humidity is about 80%, and intensity of illumination is controlled at the 1000-5000 lux.Gather after the maturation.

The present invention not only can strengthen the production capacity of excellent species greatly; reduce production costs; improve the output of cordyceps militaris sporocarp; promote scale and the industrialization production of Chinese caterpillar fungus; and because suspension has very strong homogeneity, therefrom sampling has better representativeness, and sampling is planted experimentally; can further confirm the productivity of bacterial classification, guarantee the stability of producing.In for the second time pre-incubation, cordyceps species is is only nourished and grown in dark surrounds, mycelium changes reproductive growth simultaneously over to subsequently, therefore the fruit body synchronous growth in the culture vessel, the maturity unanimity, thickness, length are even in appearance, but timely collecting, both shorten the grown cultures cycle of Chinese caterpillar fungus, guaranteed the stability of product quality again.Simultaneously in for the second time pre-incubation, culture vessel can be concentrated stacking, takes up room, the place is few, can improve the factory building availability.

Embodiment

The method of raising cordyceps production stability of the present invention is selected excellent species (in the test tube) for use, introduces secondary and cultivates in advance, improves the stability of Cordyceps militaris growth, and its concrete steps are:

A, scrape the mycelium of getting chamfered surface, be inoculated in the liquid nutrient medium, suspension culture 2～3 days is introduced pre-for the first time the cultivation, and 1 test tube strains can be inoculated 5-8 bottle suspension.

B, sampling are planted experimentally, and all the other are put in the 3-5 ℃ of refrigerator and preserve.Must put into refrigerator after the sampling, the time of preserving in refrigerator preferably is no more than 60 days at once.

After c, the affirmation bacterial classification productivity, the taking-up sampling is planted experimentally the suspension of acting normally and is inoculated, and eliminates other suspension.Get suspension 1.5-2ml during inoculation and be inoculated in the liquid nutrient medium, suspension culture 4 days obtains liquid spawn.Every bottle of switchable 100 bottles of liquid spawns of suspension.

D, with liquid-spawn inoculation to rice medium, under the dark condition of pre-incubation chamber, carry out the second time and pre-cultivate dark culturing 3-4 days.

E, transfer in the culturing room after the continuous illumination 3 days, do daylight every day, the alternately cultivation of dark each illumination-dark of 12 hours in evening, incubation time 40-45 days, culturing room's temperature was 18-20 ℃, humidity is about 80%, and intensity of illumination is controlled at the 1000-5000 lux.Gather after the maturation.

The medium of suspension culture and condition of culture are prior art among the present invention.

Wherein the condition of suspension culture among step a, the c is: dark, 18-20 ℃, 120 rev/mins.Its medium is: in every liter of potato glucose liquid nutrient medium, add potassium dihydrogen phosphate 1 gram, and magnesium sulfate 0.5 gram, hairtail peptone 2.5 grams, pH value transfers to 6.2, autoclaving 15 minutes.

Wherein the composition of rice medium is: be made up of Jiangsu rice and nutrient solution, 25 gram rice mixed the back autoclaving 30 minutes with the 35ml nutrient solution.Phosphoric acid potassium dihydrogen 2 grams per liters in the nutrient solution, magnesium sulfate 1 grams per liter, hairtail peptone 3 grams per liters, pH value transfers to 7.0.

Claims

1, a kind of method that improves cordyceps production stability is characterized in that: select excellent species for use, introduce secondary and cultivate in advance, improve the stability that Cordyceps militaris produces, concrete steps are:

A, scrape the mycelium of getting chamfered surface, be inoculated in the liquid nutrient medium suspension culture 2～3 days;

B, suspension sampled plant experimentally, all the other are put into 3-5 ℃ of refrigerator and preserve;

After c, the affirmation bacterial classification productivity, the taking-up sampling is planted experimentally the suspension of acting normally and is inoculated, and eliminates other suspension.Get suspension 1.5-2ml during inoculation and be inoculated in the liquid nutrient medium, suspension culture 4 days obtains liquid spawn;

D, with liquid-spawn inoculation to rice medium, in pre-incubation chamber, cultivated under the dark condition 3-4 days;

E, transferred to culturing room's continuous illumination 3 days, make illumination-dark afterwards and alternately cultivate, gather after the maturation.

2, the method for raising cordyceps production stability according to claim 1 is characterized in that: when sampling is planted experimentally, must put into refrigerator after the sampling, the time of preserving in refrigerator is no more than 60 days at once.

3, the method for raising cordyceps production stability according to claim 1 is characterized in that: illumination-dark alternately cultivate be every day daylight, evening dark culturing each hocketed in 12 hours, incubation time is 40～45 days.