CN1679389A - Dune fixing method of ion-beam modified vegetation, absorbent and Ramaria linked cultured plant - Google Patents
Dune fixing method of ion-beam modified vegetation, absorbent and Ramaria linked cultured plant Download PDFInfo
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- CN1679389A CN1679389A CN 200410014485 CN200410014485A CN1679389A CN 1679389 A CN1679389 A CN 1679389A CN 200410014485 CN200410014485 CN 200410014485 CN 200410014485 A CN200410014485 A CN 200410014485A CN 1679389 A CN1679389 A CN 1679389A
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Abstract
A method for fixating sand includes such steps as preparing the culture medium from soil, nutrients, water and water absorbent through mixing, loading it in the perforated bowls, choosing the seeds of drought-resistant herbaceous plant or suffurticosa plant, using ion beam to induce their mutagenesis, sowing them in said culture medium, and transplanting them along with the perforated bowls in the desert for fixating sand.
Description
Technical field
The present invention relates to desertificated area ecological environment treatment field, a kind of specifically the desert area cultivated plant, with the method for preventing and fixing sand.
Background technology
There are 168.9 ten thousand square kilometres of desertification lands in China, and develops with annual 1.32% speed, and the annual direct losses that cause because of desertification reach 54,000,000,000 yuan, nearly 1.5 hundred million yuan of average every day.Desertization has become the restraining factors of Chinese society, economic development.Desertification control method commonly used at present roughly is divided into chemical sand control, engineering sand control and biological sand control.
Mention recovery, improvement desertification sand ground or desert, people are easy to expect plant trees, plant grass.But the desert area annual precipitation is few, and year evaporation discharge is big, the air drying, and moisture is the most critical factor of restriction sand-fixation plant seed germination; The common nutrition of desert area is poor, and the mineral matter nutritional composition is few, influences plant growing; Psammophytes is because the long-term result who adapts to dry environment causes the densification of seed quality, and moisture oxygen divides very difficult visible through the seed coat, the difficulty of emerging; Though because the desert area spring precipitation, day-light saving time is long, light is strong, and ultraviolet radiation is strong, if the spring cultivation seedling can only be dried up by sunlight, or because its root system is not deep into damp sand bed as yet, can only be withered.So very difficult the desert area cultivated plant, survival rate is extremely low.
Vegetation fixes the sand to be still the most effective from now on sand control mode, mainly carry out the research and the application of single technology both at home and abroad, preferred as psammophytes group, the screening of drought-enduring psammophytes, water-absorbing resin coating psammophytes seed, water-absorbing resin and psammophytes seed mixed seeding etc., these methods can't solve psammophytes seed germination and needed long-time moisture support of plant strain growth and mineral nutrition source.
Therefore, for administering the desert, set up desert vegetation in the world, preventing and fixing sand also can not find ideal method.
Summary of the invention
The purpose of this invention is to provide a kind of ion beam improvement vegetation and water absorbing agent, Ramaria linked cultured plant tune fixing method, medium is gone in taking and placing in nutritive cube, water absorbing agent wherein makes medium have water retaining function, soil beneficial microbe wherein such as clump branch bacterium make plant in process of growth, create the root system of plant part nutrient is had stronger absorbing capacity, stronger adaptive capacity to lean soil, the annidation that strengthens, for plant provides good rhizosphere environment, for large tracts of land field planting plant on the sand ground provides favourable guarantee.
The objective of the invention is to be achieved through the following technical solutions.
Ion beam improvement vegetation and water absorbing agent, the Ramaria linked cultured plant tune fixing method, be to select drought-enduring draft, the shrub plant seed, carry out ion beam and inject mutagenesis, insert in the nutritive cube then, medium is arranged in the nutritive cube, medium is can be for the soil of herbaceous plant growth, nutrient raw material and moisture, and mix and stir with water absorbing agent, the useful rhizospheric microorganism of inoculation in the soil, with nutritive cube in the spring, cultivate autumn in the desert, the length of nutritive cube is as the criterion to reach or to stretch into damp sand bed, make plant seed rely on medium to germinate, growth, and root system can stretch out by the perforate from nutritive cube and arrive damp sand bed growth, sets up the water absorbing capacity of self, reaches the effect of fixing the sand.
Described tune fixing method is characterized in that described plant seed is Radix Glycyrrhizae, clover, clover, sea-buckthorn, foreign grass seed, carries out 25keV, 4.68 * 10 to above-mentioned seed
16～6.24 * 10
16Ions/cm
2N
+Inject.
Described tune fixing method, it is characterized in that described plant seed is meant carries out 25keV, 4.68 * 10 to Radix Glycyrrhizae, clover, clover, sea-buckthorn, foreign grass seed
16～6.24 * 10
16Ions/cm
2N
+After the injection, investigate its germination rate, growth potential, the seed of the resistant mutants that screening obtains.
Described tune fixing method is characterized in that described useful rhizospheric microorganism is clump branch bacterium, phosphate solubilizing bacteria, potassium solubilizing bacteria.
Described tune fixing method is characterized in that described useful rhizospheric microorganism is mycorhiza bacterium GM and GV.
Described tune fixing method, it is characterized in that described medium is a sand, soil, nutrient solution, the mixture of water absorbing agent, described water absorbing agent is a KD-1 type water-absorbing resin, nutrient solution is the dilution of hoagland nutrient solution, and its concentration is 30～40% of stoste, and nutritive cube is the strip cup, the bottom has a lot of apertures, and length is as the criterion to meet or exceed desert area dry sand layer thickness.
Described tune fixing method, the proportioning that it is characterized in that sand, soil, water-absorbing resin, nutrient solution in the described medium is: 60～70 (milliliters): 30～40 (milliliters): 1～1.3 (gram): 60～70 (milliliters), the total measurement (volume) of described medium are 400～1000 milliliters.
Described tune fixing method is characterized in that being added with rare earth element in the described medium.
Described tune fixing method, it is characterized in that the medium length in the described nutritive cube is 30 centimetres, contain sandy soil 600ml, 2/3 sand+1/3 soil wherein, KD-1 type water-absorbing resin 6g, plant seed germination and 1/3 required concentration hoagland nutrient solution 375ml of seedling growth, after mixing thoroughly, 121 ℃ of autoclavings, 2h, arbuscular mycorrhizal fungus Glomus mosseae and Glomus versiform are being inoculated apart from native face 3-4cm place in the cooling back, and the inoculating spores number is a 100-200/nutritive cube, put into plant seed 3-4 grain during use, sowing depth 1～2cm.
The filling of the medium in the nutritive cube can be artificial, also can design specialized mechanization filling.The nutritive cube that fills goes to cover the back and is planting in sand ground by certain seeding row spacing.
Of the present invention studies show that is with 25keV 4.68 * 10
16Ions/cm
2N
+The Radix Glycyrrhizae seed is carried out pre-treatment can improve the survival rate of seed germination and the characteristics of drought tolerance of seedlings root preferably, can be used for the Radix Glycyrrhizae seed pre-treatment of desert vegetation.Radix Glycyrrhizae after the nitrogen ion injection of different parameters is carried out the screening of PEG.Selected more drought-enduring Radix Glycyrrhizae plant 10 strains, mutagenesis goes out a strain " gold leaf " Radix Glycyrrhizae.
Sea-buckthorn has hard shell, sprouts very difficult.Usually way is to handle with the concentrated sulfuric acid, uses 25keV again, 4.68 * 10
16～6.24 * 10
16Ions/cm
2N
+After the injection, hippophae rhamnoides seed can shift to an earlier date sprouting in 4～6 days.
The Radix Glycyrrhizae that the present invention selects for use, sea-buckthorn, mycorhiza bacterium are cultivated in nutritive cube, outdoor husky pond result shows that this technology need not extraneous rainwater in two months and waters in plantation, Radix Glycyrrhizae is germination and growth normally, emergence rate is more than 95%, the Radix Glycyrrhizae planting percent is 62.9%, and stem high energy reaches about 10cm, and most of root system tensible is in nutritive cube damp sand bed in addition, tentatively has the sand ground adaptive capacity
Existing commercially available homemade water absorbing agent and husky coupling that the present invention selects for use are not hardened, and gas permeability is better, and plant, microorganism are had no side effect, and be good with plant, microorganism compatibility.
After water absorbing agent that the present invention selects for use and the sandy soil in the matrix mixed, its pH value was about neutrality, planted the plant in the sandy soil of water absorbing agent are arranged, and the plant root has masses of sand when pulling up, and does not shake.Water flushing can be with the sand flush away, but masses of water absorbing agent is firmly attached on the root.Under disecting microscope, visible licorice passes water absorbing agent, and not significantly boundary shows good compatibility between root and the water absorbing agent.Even water absorbing agent dehydration, interface are very unobvious yet, root is not damaged.
Plant seed is carried out 4.68 * 10
16～6.24 * 10
16Ions/cm
2N
+Inject, can improve the survival rate of seed germination and the characteristics of drought tolerance of seedlings root preferably,
The selected herbaceous plant of the present invention has Radix Glycyrrhizae, ocean grass, clover, and shrub has sea-buckthorn, dry land willow, and arbor is had a mind to poplar etc.
Of the present invention studies show that, arbuscular mycorrhizal fungi can be by helping to absorb Soil Mineral Nutrition such as phosphorus etc., promoting the herbaceous plant growth and help improve soil structure, improve stress resistance of plant such as drought resisting simultaneously, resist characteristics such as saline and alkaline, disease-resistant, is the mycorrhizal fungi that using value is arranged very much.
The present invention filters out microbial strains GM and the GV that two strains promote plant growing.They can obviously promote plant growings such as Radix Glycyrrhizae, and wherein GM and GV make Radix Glycyrrhizae biomass (strain is heavy) increase by 2.77 times, 1.25 times; The root infection rate reaches 51.5% and 50.5% respectively.At microscopically, the microorganism mycelia is along the Radix Glycyrrhizae root growth, and the mycelia that has is deep into root inside along the space between cells, and forms spore.Illustrate that microorganism and Radix Glycyrrhizae have good symbiotic relation.Licorice is partly secreted material such as organic acid provides nutrition for growth of microorganism, extreme condition microorganism fixed nitrogen, phosphorus decomposing, potassium decomposing, and for the Radix Glycyrrhizae growth provides nutrient, licorice polysaccharide content obviously improves behind the microbe inoculation.Along with the raising of polyoses content, the drought resistance of Radix Glycyrrhizae also improves.
To have indigenous arbuscular mycorrhizal fungi at sand ground few in view of arbuscular mycorrhizal fungi, the content of organic matter is low, nutrient content is low, features such as soil structure is poor, some area is saline and alkaline partially, therefore the active arbuscular mycorrhizal fungi that adds in the nutritive cube of the present invention is to the stronger absorbing capacity of nutrient, to the stronger adaptive capacity of lean soil, and very strong annidation, can be for plant provides good rhizosphere environment, for large tracts of land field planting plant on the sand ground provides favourable guarantee.Also do not see at present the same class methods and the product of exploitation at home as yet.The leguminous plant of cultivating and growing of the present invention such as Radix Glycyrrhizae, clover, sea-buckthorn etc. can quicken the recovery in sand ground nitrogen storehouse simultaneously, and this is vital to setting up a continual and steady ecosystem.
Arbuscular mycorrhizal fungus energy and root system of plant form good symbiotic relation in the nutritive cube, the mineral nutrition that growth by a large amount of symbiosis mycelia will decompose, transform offers the plant growing utilization for a long time, satisfies the nutritional requirement of plant growing, overcome that plant can directly utilize oligotrophy, the difficult problem that soil fertility is low under the sand ground condition; The arbuscular mycorrhizal fungus of field planting can improve plant crown root ratio by plant growing simultaneously, helping young leaves elevates above the soil and carries out photosynthesis, promote plant nutrition and reproductive growth, promote plant quality, have effects such as the plant salt endurance of raising, drought resistance, disease resistance simultaneously, this is the field planting of large tracts of land plant, the ecosystem of recover, improvement or upgrade being degenerated can play sound assurance.
Because the inwall of nutritive cube has crossed water-retaining film, has only the upper port of nutritive cube with the sectional area that extraneous dry air directly contacts, alms bowl inner macromolecule absorbent material contains the above required complete nutrition liquid of plant growing of 200ml, therefore, nutritive cube evaporation discharge in the environment of drying is minimum, and contained humidity and nutrition can provide 75 days for the growth of Radix Glycyrrhizae seed germination and seedling and special microorganism spore germination, mycelia and plant symbiosis～and 90 days moisture and nutritional support need not extraneous rainwater and nutrition.Horqin sand ground general late June enters the rainwater phase, and the major part of annual precipitation 340ml concentrates on for 7～August.This nutritive cube is general plants around the mid-April, can spend spring, early summer arid season short of rain.Licorice ties up to and can grow more than the long 30cm in the nutritive cube during this section, enter rainy season this moment, dry sand layer becomes damp sand bed, because the signal induction of moisture and the hydrotropism of root system self, root system can pass through cavity, nutritive cube bottom, passes nutritive cube and enters damp sand bed, through the growth in rainwater season in 7～August, root system is distributed with certain zone in damp sand bed, self had dark sand bed moisture of certain absorption and drought-resistance ability.Grains of sand mineral can be decomposed, be transformed to nutritive cube interior application special microorganism such as clump branch bacterium on the one hand is the required mineral nutrition of plant growing, can form good symbiotic relation with root system of plant again on the other hand, can offer the Radix Glycyrrhizae growth by the mineral nutrition that a large amount of symbiosis mycelia will decompose, transform for a long time and utilize, satisfy the nutritional requirement of Radix Glycyrrhizae growth.
Description of drawings
Fig. 1 is the nutritive cube structural representation.
Fig. 2 is that plant absorbs the moisture and the schematic diagram of nourishing and growing at nutritive cube.
Fig. 3 is that plant root is to damp sand bed growth schematic diagram.
Fig. 4 is that plant root is to damp sand bed growth schematic diagram.
Fig. 5 is plant root to damp sand bed growth and sets up self water absorbing capacity schematic diagram.
Embodiment
1, selects Radix Glycyrrhizae, clover, clover, sea-buckthorn, foreign grass seed.
The Radix Glycyrrhizae seed is carried out 25keV, 4.68 * 10
16Ions/cm
2N
+Inject.And the Radix Glycyrrhizae seed after ion injected cultivates, and investigates its germination rate, growth potential, selected more drought-enduring Radix Glycyrrhizae plant 10 strains, and mutagenesis goes out a strain " gold leaf " Radix Glycyrrhizae.
Sea-buckthorn has hard shell, sprouts very difficult.Handle with the concentrated sulfuric acid earlier, use 25keV again, 4.68 * 10
16～6.24 * 10
16Ions/cm
2N
+Inject.
Clover, clover, foreign grass seed are carried out 25keV, 4.68 * 10
16～6.24 * 10
16Ions/cm
2N
+Inject.
2, the nutritive cube outward appearance of the present invention of making is square cuboid, length of side 4cm, length 30cm, it is the 5mm circular hole that 12～20 diameters are left in the bottom, the matrix of filling in the nutritive cube is sandy soil 400ml, 2/3 sand+1/3 soil wherein, the KD-1 type water-absorbing resin 4g that Kaida Industry (Group) Co Ltd, Qingdao produces, 121 ℃ of the required 1/3 concentration hoagland nutrient solution 250ml autoclavings of plant seed germination and seedling growth, 2h, arbuscular mycorrhizal fungus Glomus mosseae and Glomus versiform are being inoculated apart from native face 3-4cm place in the cooling back, the inoculating spores number is a 100-200/packing, put into Radix Glycyrrhizae during use respectively, clover, clover, sea-buckthorn or foreign careless plant seed 3-4 grain, sowing depth 1cm.
This nutritive cube was planted in the desert, Horqin by certain plant spacing before and after general mid-April, can spend spring, early summer arid season short of rain.Radix Glycyrrhizae, clover, clover, sea-buckthorn or foreign grass roots tie up in the nutritive cube and can grow more than the long 30cm during this section, and Horqin sand ground general late June enters the rainwater phase, and the major part of annual precipitation 340ml concentrates on for 7～August.At this moment Shamian Island is damp sand bed below 30 centimetres.Because the signal induction of moisture and the hydrotropism of root system, root system is grown downwards, sets up the water absorbing capacity of self, thereby sets up Radix Glycyrrhizae, clover, clover, sea-buckthorn and foreign careless vegetation, reaches sand fixation.
Can add rare earth element in the described medium.
Claims (9)
1, ion beam improvement vegetation and water absorbing agent, the Ramaria linked cultured plant tune fixing method, be to select drought-enduring draft, the shrub plant seed, carry out ion beam and inject mutagenesis, insert in the nutritive cube then, medium is arranged in the nutritive cube, medium is can be for the soil of herbaceous plant growth, nutrient raw material and moisture, and mix and stir with water absorbing agent, the useful rhizospheric microorganism of inoculation in the soil, with nutritive cube in the spring, cultivate autumn in the desert, the length of nutritive cube is as the criterion to reach or to stretch into damp sand bed, make plant seed rely on medium to germinate, growth, and root system can stretch out by the perforate from nutritive cube and arrive damp sand bed growth, sets up the water absorbing capacity of self, reaches the effect of fixing the sand.
2, tune fixing method according to claim 1 is characterized in that described plant seed is Radix Glycyrrhizae, clover, clover, sea-buckthorn, foreign grass seed, carries out 25keV, 4.68 * 10 to above-mentioned seed
16～6.24 * 10
16Ions/cm
2N
+Inject.
3, tune fixing method according to claim 1, it is characterized in that described plant seed is meant carries out 25keV, 4.68 * 10 to Radix Glycyrrhizae, clover, clover, sea-buckthorn, foreign grass seed
16～6.24 * 10
16Ions/cm
2N
+After the injection, investigate its germination rate, growth potential, the seed of the resistant mutants that screening obtains.
4, tune fixing method according to claim 1 is characterized in that described useful rhizospheric microorganism is clump branch bacterium, phosphate solubilizing bacteria, potassium solubilizing bacteria.
5, tune fixing method according to claim 4 is characterized in that described useful rhizospheric microorganism is mycorhiza bacterium GM and GV.
6, tune fixing method according to claim 1, it is characterized in that described medium is a sand, soil, nutrient solution, the mixture of water absorbing agent, described water absorbing agent is a KD-1 type water-absorbing resin, nutrient solution is the dilution of hoagland nutrient solution, and its concentration is 30～40% of stoste, and nutritive cube is the strip cup, the bottom has a lot of apertures, and length is as the criterion to meet or exceed desert area dry sand layer thickness.
7, tune fixing method according to claim 6, the proportioning that it is characterized in that sand, soil, water-absorbing resin, nutrient solution in the described medium is: 60～70 (milliliters): 30～40 (milliliters): 1～1.3 (gram): 60～70 (milliliters), the total measurement (volume) of described medium are 400～1000 milliliters.
8, according to claim 1,6 or 7 described tune fixing methods, it is characterized in that being added with rare earth element in the described medium.
9, tune fixing method according to claim 6, it is characterized in that the medium length in the described nutritive cube is 30 centimetres, contain sandy soil 600ml, 2/3 sand+1/3 soil wherein, KD-1 type water-absorbing resin 6g, plant seed germination and 1/3 required concentration hoagland nutrient solution 375ml of seedling growth, after mixing thoroughly, 121 ℃ of autoclavings, 2h, arbuscular mycorrhizal fungus Glomus mosseae and Glomus versiform are being inoculated apart from native face 3-4cm place in the cooling back, and the inoculating spores number is a 100-200/nutritive cube, put into plant seed 3-4 grain during use, sowing depth 1～2cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100144856A CN100334934C (en) | 2004-03-27 | 2004-03-27 | Dune fixing method of ion-beam modified vegetation, absorbent and Ramaria linked cultured plant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100144856A CN100334934C (en) | 2004-03-27 | 2004-03-27 | Dune fixing method of ion-beam modified vegetation, absorbent and Ramaria linked cultured plant |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1679389A true CN1679389A (en) | 2005-10-12 |
| CN100334934C CN100334934C (en) | 2007-09-05 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2004100144856A Expired - Fee Related CN100334934C (en) | 2004-03-27 | 2004-03-27 | Dune fixing method of ion-beam modified vegetation, absorbent and Ramaria linked cultured plant |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102511278A (en) * | 2011-12-16 | 2012-06-27 | 北京丹路实业公司 | Desert control technique using special microbial fertilized psammophyte fertilizer |
| CN102763532A (en) * | 2011-05-05 | 2012-11-07 | 北京仁创铸造有限公司 | Sand-stabilizing planting method |
| CN103392459A (en) * | 2013-06-18 | 2013-11-20 | 中国矿业大学(北京) | Planting method for improving surviving rate of sand seedlings |
| CN105706888A (en) * | 2016-02-03 | 2016-06-29 | 华北水利水电大学 | Desert plant cultivation device and sand stabilization and water retaining method |
| CN106258057A (en) * | 2016-08-09 | 2017-01-04 | 中国矿业大学(北京) | Arbuscular mycorrhiza and phosphate solubilizing bacteria work in coordination with promotion plant growing and the method for soil improvement |
| CN109526469A (en) * | 2018-12-26 | 2019-03-29 | 中国科学院植物研究所 | A kind of nutritive cube for meadow sheep's hay field planting of degenerating |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09220035A (en) * | 1996-02-19 | 1997-08-26 | Japan Atom Energy Res Inst | Method for crossbreeding by ion beam irradiation |
-
2004
- 2004-03-27 CN CNB2004100144856A patent/CN100334934C/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102763532A (en) * | 2011-05-05 | 2012-11-07 | 北京仁创铸造有限公司 | Sand-stabilizing planting method |
| CN102763532B (en) * | 2011-05-05 | 2015-05-13 | 北京仁创砂业科技有限公司 | Sand-stabilizing planting method |
| CN102511278A (en) * | 2011-12-16 | 2012-06-27 | 北京丹路实业公司 | Desert control technique using special microbial fertilized psammophyte fertilizer |
| CN103392459A (en) * | 2013-06-18 | 2013-11-20 | 中国矿业大学(北京) | Planting method for improving surviving rate of sand seedlings |
| CN103392459B (en) * | 2013-06-18 | 2015-10-28 | 中国矿业大学(北京) | A kind of method for planting improving sand ground Survival rate of nursery stock |
| CN105706888A (en) * | 2016-02-03 | 2016-06-29 | 华北水利水电大学 | Desert plant cultivation device and sand stabilization and water retaining method |
| CN106258057A (en) * | 2016-08-09 | 2017-01-04 | 中国矿业大学(北京) | Arbuscular mycorrhiza and phosphate solubilizing bacteria work in coordination with promotion plant growing and the method for soil improvement |
| CN109526469A (en) * | 2018-12-26 | 2019-03-29 | 中国科学院植物研究所 | A kind of nutritive cube for meadow sheep's hay field planting of degenerating |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100334934C (en) | 2007-09-05 |
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