CN1621837A - Preparation of specifically indicating erythrocyte and use in micro column immunological experimental technology - Google Patents
Preparation of specifically indicating erythrocyte and use in micro column immunological experimental technology Download PDFInfo
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- CN1621837A CN1621837A CN 200310115516 CN200310115516A CN1621837A CN 1621837 A CN1621837 A CN 1621837A CN 200310115516 CN200310115516 CN 200310115516 CN 200310115516 A CN200310115516 A CN 200310115516A CN 1621837 A CN1621837 A CN 1621837A
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Abstract
The new immunology test and detection method different from traditional hemagglutination test and conventional microcolumn gel test adopts double-hydroformylation reagent to cure human erythrocyte and combining specific antibody onto membrane, can preserve specific indication erythrocyte at 18-25 deg.c for one year, and may be used in the detection of blood cell, pathogenic microbe, various biological molecules, various antigen and antibody, etc. The method is simple, accurate, sensitive and repeatable, and may be used in detection in clinical medicine and preclinical medicine.
Description
The present invention's " specificity indication red blood cell preparation and application in micro-column gel immunization experiment technology thereof " belongs to the medical biotechnology field of biological product.
The present invention uses dialdehyde reagent solidifies the Freshman erythrocyte membrane, emerging aldehyde radical and specific antibody are in the proper temperature combination on the film, after the physiological saline washing, add antiseptic and 6% concentration albumin and be prepared into specificity indication red blood cell, stability test proof indication red blood cell can be in room temperature 18-25 ℃ long preservation, the red blood cell dissolving of not breaking.The indication erythrocyte membrane on specific antibody can with the corresponding antigens combination, its specific antigen of difference according to antibody can be human blood cells such as red blood cell, blood platelet, leucocyte, pathogenic microorganisms such as bacterium, virus, conveyor screw also can be soluble antigen such as medicine, hormone, blood factor, antibody and the antiantibodys etc. that are attached to erythrocyte surface.More than each system's antigen-antibody reaction all in the anti-people's ball of microtrabeculae glue, carry out, rather than in the test tube liquid medium, carry out.After 80g-300g was centrifugal, antigen antibody complex matter promptly formed network-like red blood cell grumeleuse, promptly indicated red blood cell-antigen-specific antibody-anti-people's ball-specific antibody-antigen-indication red blood cell
Be arranged in glue surface or glue, be referred to as positive reaction, not in conjunction with corresponding antigens, then can not form compound as specific antibody, promptly can not form grumeleuse, single erythrocyte sedimentation is in microtrabeculae pipe vertex (vertices) portion.
It is different to carry out traditional hemagglutination test in the present invention and the liquid medium, and red blood cell and corresponding antigens are the immune responses of carrying out in colloidal medium among the present invention.The present invention is different with conventional micro-column gel immuno analytical method, and conventional method can only be used new red blood cell and carry out detection of antibodies, and fresh red blood cell can not long preservation, and the present invention indicate red blood cell be become after treatment can long preservation red blood cell.
The present invention carries out combination with the advantage of traditional hemagglutination test and conventional gel test, has overcome shortcoming separately again, has set up a kind of new immunological experiment method of inspection innovatively.Make it more convenient, accurate, standardization result, susceptibility are improved rightly, and good reproducibility, the easy preservation of result etc.More favourable basic medical and clinical to various blood cells, the immunology detection of pathogenic microorganism and various biomolecule.
Accompanying drawing: specificity indication red blood cell preparation and the application in micro-column gel immunization experiment technology thereof.
Claims (5)
1, is different from the erythrocytic preparation of specificity of traditional hemagglutination test and conventional micro-column gel test and in micro-column gel immunization experiment technology, be applied to multiple antigen-antibody and detect the new immunological experiment method of inspection.
2, indicate erythrocytic preparation method as follows according to right 1 described specificity:
Through screening cell and the negative healthy people O type red blood cell application dialdehyde reagent of spectrum cell detection emerging aldehyde radical and specific antibody on its curing → film are indicated red blood cell at proper temperature combination → physiological saline washing → adding antiseptic and 6% concentration albumin → specificity.
3, under 18-25 ℃ of condition, preserved 1 year the red blood cell dissolving of not breaking, but long preservation according to the described specificity indication of right 1 red blood cell.
4, according to the specific antibody on the described specificity indication of right 1 red blood cell can with the corresponding antigens combination, difference according to antibody, can be used for detecting human blood cells such as red blood cell, blood platelet, leucocyte, pathogenic microorganisms such as bacterium, virus, conveyor screw also can be used for detecting the soluble antigen such as the antigen-antibodies such as medicine, hormone, blood factor, antibody and antiantibody that are attached to erythrocyte surface.
5, judge as follows according to right 1 described specificity indication red blood cell detection antigen-antibody experimental technique and result:
(1) detection method: antigen-antibody to be checked is added in the microtrabeculae pipe, 80-300g is centrifugal, and antigen antibody complex promptly forms network-like red blood cell grumeleuse promptly: promptly indicate red blood cell-antigen-specific antibody-anti-people's ball-specific antibody-antigen-indication red blood cell
(2) result judges:
Positive reaction: red blood cell is arranged in glue surface or glue.
Negative reaction: erythrocyte sedimentation is in microtrabeculae tip bottom.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200310115516 CN1621837A (en) | 2003-11-28 | 2003-11-28 | Preparation of specifically indicating erythrocyte and use in micro column immunological experimental technology |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200310115516 CN1621837A (en) | 2003-11-28 | 2003-11-28 | Preparation of specifically indicating erythrocyte and use in micro column immunological experimental technology |
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| CN1621837A true CN1621837A (en) | 2005-06-01 |
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| CN 200310115516 Pending CN1621837A (en) | 2003-11-28 | 2003-11-28 | Preparation of specifically indicating erythrocyte and use in micro column immunological experimental technology |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103344755A (en) * | 2013-07-19 | 2013-10-09 | 中国科学院苏州生物医学工程技术研究所 | Method for preparing magnetized and hydroformyled sheep red blood cell |
| CN104965079A (en) * | 2015-06-27 | 2015-10-07 | 江阴金悦达生物技术有限公司 | Novel micro-column gel test HIV antibody detection reagent kit and manufacturing method thereof |
| CN108956602A (en) * | 2018-08-02 | 2018-12-07 | 石家庄久正生物科技有限公司 | A kind of brucellosis detection card and preparation method thereof |
| WO2021155631A1 (en) * | 2020-02-08 | 2021-08-12 | 苏州大学 | Erythrocyte gel delivery system, and preparation method therefor and application thereof |
-
2003
- 2003-11-28 CN CN 200310115516 patent/CN1621837A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103344755A (en) * | 2013-07-19 | 2013-10-09 | 中国科学院苏州生物医学工程技术研究所 | Method for preparing magnetized and hydroformyled sheep red blood cell |
| CN103344755B (en) * | 2013-07-19 | 2015-06-24 | 中国科学院苏州生物医学工程技术研究所 | Method for preparing magnetized and hydroformyled sheep red blood cell |
| CN104965079A (en) * | 2015-06-27 | 2015-10-07 | 江阴金悦达生物技术有限公司 | Novel micro-column gel test HIV antibody detection reagent kit and manufacturing method thereof |
| CN108956602A (en) * | 2018-08-02 | 2018-12-07 | 石家庄久正生物科技有限公司 | A kind of brucellosis detection card and preparation method thereof |
| WO2021155631A1 (en) * | 2020-02-08 | 2021-08-12 | 苏州大学 | Erythrocyte gel delivery system, and preparation method therefor and application thereof |
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