CN103344755A - Method for preparing magnetized and hydroformyled sheep red blood cell - Google Patents
Method for preparing magnetized and hydroformyled sheep red blood cell Download PDFInfo
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Abstract
The invention discloses a method for preparing a magnetized and hydroformyled sheep red blood cell. The method comprises the following steps of: preparing nano magnetic beads; preparing a hydroformyled sheep red blood cell; preparing a magnetized and hydroformyled sheep red blood cell; coating protein on the magnetized and hydroformyled sheep red blood cell; detecting a sample; and observing the result. The prepared magnetized and hydroformyled sheep red blood cell can be used as an indicator cell of an agglutination test, a carrier, enzyme and a chemiluminescence matter of an antigen antibody agglutination test, or a carrier detected in a fluorescent material marking test. The magnetized and hydroformyled sheep red blood cell prepared by the method is long in retention period, simultaneously has paramagnetism, can achieve rapid aggregation and redispersion, avoids complicated centrifuge processes in the traditional agglutination test, and can be used for preparing artificial granular antigen antibodies with uniform sizes; coating and detection reaction are carried out in a uniform phase after a solid-phase carrier is replaced; and the method is full in action, high in sensitivity, low in cost than an elisa plate, and is mainly applied to a screening or quantitative detection test of a special red blood cell antibody and other soluble antigen antibodies.
Description
Technical field
The present invention relates to biological technical field, particularly relate to a kind of preparation method of magnetizing the hydroformylation sheep red blood cell.
Background technology
Agglutinating reaction is a kind of serological reaction, process is that particulate antigen (complete pathogenic microorganism or red blood cell etc.) is combined with corresponding antibodies, under the condition that has electrolyte to exist, through certain hour, macroscopic aggegation fritter occurs, agglutinating reaction can be divided into direct agglutination reaction and indirect agglutination two classes.
Direct agglutination reaction is that graininess antigen (as bacterium, red blood cell etc.) is direct in conjunction with the agglutination phenomenon that occurs with corresponding antibodies, mainly is divided into slide method and test tube method.Slide method is a kind of qualitative test method, and available known antibodies detects unknown antigen.Test tube method is a kind of classical way of quantitative test, and available known antigens detects the content that has or not certain antibody and antibody in the serum, is used for assisting clinical diagnosis or supplies epidemiological investigation.
Indirect agglutination is adsorbed in soluble antigen (or antibody) a kind of earlier and immunity is irrelevant, the surface of sized granular carrier, make artificial type particulate antigen, act on corresponding antibodies (or antigen) then, under the suitable condition that has dielectric to exist, aggegation can take place.The microballoon that is used as carrier can be with natural corpuscular property material; red blood cell, activated carbon granule or alumina silicate particle etc. as people's (O type) and animal (sheep, rabbit etc.); also available artificial synthetic or natural macromolecular material is made, as the polystyrene latex microballoon etc.Because carrier granular has increased the reaction area of soluble antigen, when the antigen on the particle after micro-antibody is combined, just be enough to macroscopic reaction occur, susceptibility is more much higher than direct agglutination reaction.
Granular pattern antigen in the agglutinating reaction, what use is new fresh cell or bacterium, all there is unsuitable long preservation, and defective such as complex operation, though the agglutination test testing result is more directly perceived, but the problem that has the low and poor accuracy of sensitivity, and experimental result is difficult for standardization, and this uses in the application of the immunology detection of conventional antigen-antibody reaction agglutinating reaction and has caused certain obstacle.Therefore, send out detection techniques such as exhibiting endoglucanase, chemiluminescence or fluorescence labeling on the agglutination test basis, utilize artificial type particulate matter to increase reaction area and the responsive detection means of soluble antigen, make the soluble antigen antibody test accurate more, quick.Some microballoons are made in employing at present manually synthetic or natural macromolecular material, be used for the soluble antigen detection of antibodies, but such microballoon building-up process is more loaded down with trivial details, the requirement for experiment condition harshness, buy finished product price height, and there is the size particles heterogeneity, and shortcoming such as protein labeling efficient is low, so be not widely used clinically so far.
Summary of the invention
The technical matters that the present invention mainly solves provides a kind of preparation method of magnetizing the hydroformylation sheep red blood cell, the composite particles of the present invention's preparation had both possessed the characteristic of indication particle, possess the characteristic of solid phase carrier again and can be made into artificial type particulate antigen antibody, storage life is longer, has simultaneously paramagnetism again, can realize assembling rapidly and disperseing again, avoid centrifugal process loaded down with trivial details in traditional agglutination test, very convenient, it can be used for the indicator cells as traditional agglutination test, the carrier of antigen-antibody screening agglutination test and the enzyme of antigen-antibody, the carrier of qualitative or quantitative detection in chemiluminescent substance or the fluorescent material mark test, this carrier can replace solid phase carrier and be prepared into the artificial type particulate antigen antibody of size homogeneous, wherein wrapping quilt all carries out in sparing mutually with detection reaction, not only with low cost than normal enzyme target, more feasible reaction is more abundant, and susceptibility is higher.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is: a kind of preparation method of magnetizing the hydroformylation sheep red blood cell is provided, may further comprise the steps:
(1) nanometer magnetic bead preparation;
(2) hydroformylation sheep red blood cell preparation;
(3) magnetization hydroformylation sheep red blood cell preparation;
(4) magnetization hydroformylation sheep red blood cell coating protein;
(5) sample detection;
(6) result observes.
In a preferred embodiment of the present invention, the concrete steps of described step (1) nanometer magnetic bead preparation are: preparation iron salt solutions and ammonia spirit, in the iron salt solutions for preparing, add surfactant and stirring, add the ammonia spirit for preparing then and obtain nanometer magnetic bead.
In a preferred embodiment of the present invention, the concrete steps of described step (2) hydroformylation sheep red blood cell preparation are: with physiological saline sheep red blood cell is diluted, add glutaraldehyde, leave standstill behind the mixing, unnecessary glutaraldehyde is removed in washing, and it is standby to make suspension.
In a preferred embodiment of the present invention, the concrete steps of described step (3) magnetization hydroformylation sheep red blood cell preparation are: determine the magnetization condition by preliminary experiment, hydroformylation sheep red blood cell and the vibration of nanometer magnetic bead mixing, magnetic force absorption washing then is with the red blood cell flush away that is not magnetized.
In a preferred embodiment of the present invention, the concrete steps of described step (4) magnetization hydroformylation sheep red blood cell coating protein are: will magnetize and leave standstill after hydroformylation sheep red blood cell and albumen mix, and mix with the roller bearing instrument, and then leave standstill washing, it is standby to make suspension.
In a preferred embodiment of the present invention, described nanometer magnetic bead particle diameter is 10-500 nm.
In a preferred embodiment of the present invention, the hematocrit percent concentration of described sheep red blood cell is 2-5%, and the mass percent concentration of glutaraldehyde is 1-2%, and both press the reaction volume of 1:1 than mixing.
In a preferred embodiment of the present invention, the hematocrit of described hydroformylation sheep red blood cell and nanometer magnetic bead is than being 15:1.
In a preferred embodiment of the present invention, the mass concentration of described albumen is 1-5 mg/mL, and the hematocrit percent concentration of magnetization hydroformylation sheep red blood cell is 20-50%, and the long-pending ratio of both bodies is 3:1.
In a preferred embodiment of the present invention, the carrier of described magnetization hydroformylation sheep red blood cell qualitative or quantitative detection in can enzyme, chemiluminescent substance or fluorescent material mark test as the carrier of the indicator cells of agglutination test, antigen-antibody screening agglutination test and antigen-antibody.
The invention has the beneficial effects as follows:
1, the sheep red blood cell wide material sources that the present invention relates to, with low cost, the size homogeneous, sheep red blood cell after the hydroformylation is difficult for haemolysis, can preserve the time more than 3 months, thereby be a kind of good indication particle, in traditional agglutination test, have very widely and use.
2, the present invention is incorporated into nanometer magnetic bead hydroformylation sheep red blood cell surface innovatively, form magnetization hydroformylation sheep red blood cell, a kind of like this composite particles had both possessed the characteristic of indication particle, possess the characteristic of solid phase carrier again and can be prepared to artificial type particulate antigen, storage life is longer, has paramagnetism again simultaneously, can realize assembling rapidly and disperseing, avoid centrifugal process loaded down with trivial details in traditional agglutination test, very convenient.
The indicator cells that 3, not only can be used for traditional agglutination test, also can as the carrier of antigen-antibody screening agglutination test and antigen-antibody enzyme, chemiluminescent substance or fluorescent material mark test in the carrier of qualitative or quantitative detection, wherein wrapping quilt behind the replacement solid phase carrier all carries out in sparing mutually with detection reaction, not only with low cost than normal enzyme target, can also make that reaction is more abundant, susceptibility is higher, mainly can be used for special erythrocyte blood type antibody and other soluble antigen antibody screenings or quantitatively detect test.
Description of drawings
In order to be illustrated more clearly in the technical scheme in the embodiment of the invention, the accompanying drawing of required use is done to introduce simply in will describing embodiment below, apparently, accompanying drawing in describing below only is some embodiments of the present invention, for those of ordinary skills, under the prerequisite of not paying creative work, can also obtain other accompanying drawing according to these accompanying drawings, wherein:
Fig. 1 is the figure that the scanning electron microscope of the magnetization hydroformylation sheep red blood cell that preparation method's one preferred embodiment of hydroformylation sheep red blood cell obtains is magnetized in the present invention.
Embodiment
To the technical scheme in the embodiment of the invention be clearly and completely described below, obviously, described embodiment only is a part of embodiment of the present invention, rather than whole embodiment.Based on the embodiment among the present invention, those of ordinary skills belong to the scope of protection of the invention not making all other embodiment that obtain under the creative work prerequisite.
Embodiment one:
Provide a kind of application hydroformylation magnetization sheep red blood cell that soluble antibody is detected the agglutination test model, detect other specific antibodies, the specific antigen bag that it is corresponding can be used and antibody test by to hydroformylation magnetization sheep red blood cell.Comprise the steps:
1, the preparation of nanometer magnetic bead
0.85g FeCl
36H
2O and 0.30g FeCl
24H
2O is dissolved under nitrogen protection in the 200 mL distilled water.Add proper amount of surfactant, under brute force stirs, 1.5 mol/L ammonia spirits are slowly joined in the above-mentioned solution, when the pH of solution is increased to 6 ~ 7, produce a large amount of black Fe in the solution
3O
4Particle; Continue to add ammoniacal liquor to pH=8, make hydrolysis complete.At 80 ℃ of following ageing 0.5 h.The solid that generates is separated, with distillation washing 3 times, under ultrasonication, it is dispersed in the 100 mL distilled water then, obtain Fe
3O
4Colloidal solution collect standby.
2, sheep red blood cell preparation
With the fresh sheep blood of 10 mL centrifugal 10 min under 1500 rpm, collect red blood cell, with physiological saline washing 3 times, it is standby to make 2 mL red cell suspensions again.
3, glutaraldehyde configuration
Dispose 2% concentration glutaraldehyde, 10 mL with physiological saline, standby.
4, sheep red blood cell hydroformylation
(1) with physiological saline sheep red blood cell is diluted to 5% concentration, gets 5 mL, add isopyknic 2% concentration glutaraldehyde, behind the mixing, 4 ℃ leave standstill 30 min gently;
(2) with physiological saline washing 3 times, the unnecessary glutaraldehyde of flush away, it is standby to make suspension with physiological saline.
5, hydroformylation sheep red blood cell magnetization
(1) determine the magnetization condition by preliminary experiment, hydroformylation red blood cell and magnetic bead hematocrit are respectively got 1 mL mixing vibration 2min than being 15:1;
(2) magnetic force absorption washing is with the red blood cell flush away that is not magnetized.
6, hydroformylation magnetization sheep red blood cell bag is by the blood group polypeptide antigen
(1) mass concentration of blood group polypeptide antigen is 2 mg/mL, is 7.2 PBS dilution with the pH value of 0.15M;
(2) magnetization hydroformylation sheep red blood cell is 7.2 PBS displacement and is diluted to mass percent concentration 50% with the pH value of 0.15M;
(3) magnetization hydroformylation sheep red blood cell and blood group polypeptide antigen volume ratio 3:1, both mix, and room temperature leaves standstill 30 min;
(4) mix 5 h with the roller bearing instrument, rotating speed 100 r/min;
(5) room temperature is washed 3 times with 0.15 M PH7.2 PBS after leaving standstill 30 min, and it is standby to make suspension.
7, sample detection
(1) will wrap by the hydroformylation of blood group polypeptide antigen magnetization sheep erythrocyte suspension 100 μ l in ELISA Plate hole, 96 hole, and add each 50 μ l of normal person's sample/negative sample/dummy, 1 min vibrates;
After 30 min are hatched in (2) 37 ℃ of water-baths, during the mixing that vibrates, with program control magnetization oscillator absorption washing 3 times;
(3) add goat-anti people IgM, after 30 min are hatched in 37 ℃ of water-baths, vibration mixing observations;
(4) agglutination phenomenon appears in positive findings, and feminine gender and blank then do not have agglutination phenomenon.
8, detection and interpretation as a result.
Embodiment two:
Provide a kind of application hydroformylation magnetization sheep red blood cell to be used for antibody fluoroscopic examination test model, the specific antigen bag by to hydroformylation magnetization sheep red blood cell, is used for antibody test.Comprise the steps:
1, nanometer magnetic bead preparation
0.85g FeCl
36H
2O and 0.30g FeCl
24H
2O is dissolved under nitrogen protection in the 200 mL distilled water.Add proper amount of surfactant, under brute force stirs, 1.5 mol/L ammonia spirits are slowly joined in the above-mentioned solution, when the pH of solution is increased to 6 ~ 7, produce a large amount of black Fe in the solution
3O
4Particle; Continue to add ammoniacal liquor to pH=8, make hydrolysis complete.At 80 ℃ of following ageing 0.5 h.The solid that generates is separated, with distillation washing 3 times, under ultrasonication, it is dispersed in the 100 mL distilled water then, obtain Fe
3O
4Colloidal solution collect standby.
2, sheep red blood cell preparation
The fresh sheep blood of 10 mL at centrifugal 10 min of 1500 rpm, is collected red blood cell, and with physiological saline washing 3 times, it is standby to make 2 mL red cell suspensions again.
3, glutaraldehyde configuration
Dispose 2% concentration glutaraldehyde, 10 mL with physiological saline, standby.
4, sheep red blood cell hydroformylation
(1) with physiological saline sheep red blood cell is diluted to 5% concentration, gets 5 mL, add isopyknic 2% concentration glutaraldehyde, behind the mixing, 4 ℃ leave standstill 30 min gently;
(2) with physiological saline washing 3 times, the unnecessary glutaraldehyde of flush away, it is standby to make suspension with physiological saline.
5, hydroformylation sheep red blood cell magnetization
(1) determine the magnetization condition by preliminary experiment, hydroformylation red blood cell and nanometer magnetic bead hematocrit are respectively got 1 mL mixing vibration 2min than being 15:1,
(2) magnetic force absorption washing is with the red blood cell flush away that is not magnetized.
6, hydroformylation magnetization sheep red blood cell bag is by human IgG
(1) mass concentration of IgG is 2mg/mL, is 7.2 PBS dilution with the pH value of 0.15M;
(2) hydroformylation magnetization sheep red blood cell is that to replace and be diluted to mass percent concentration be 50% dense for 7.2 PBS with the pH value of 0.15M;
(3) hydroformylation magnetization sheep red blood cell and IgG volume ratio 3:1, both mix, and room temperature leaves standstill 30 min;
(4) mix 5 h with the roller bearing instrument, rotating speed 100rpm;
(5) to leave standstill behind 30 min pH value with 0.15M be 7.2 PBS washing 3 times to room temperature, and it is standby to make suspension.
7, sample detection
(1) will wrap by the hydroformylation of human IgG magnetization sheep erythrocyte suspension in ELISA Plate hole, 96 hole;
(2) goat anti-human igg of adding FITC mark, 30 min are hatched in 37 ℃ of water-baths, wash 2 times;
(3) flow cytometer or fluorescence detector read the fluorescence signal value.
The invention has the beneficial effects as follows: the sheep red blood cell wide material sources that the present invention relates to, with low cost, the size homogeneous, sheep red blood cell after the hydroformylation is difficult for haemolysis, can preserve the time more than 3 months, thereby be a kind of good indication particle, in traditional agglutination test, have very widely and use.The present invention is incorporated into nanometer magnetic bead hydroformylation sheep red blood cell surface innovatively, form magnetization hydroformylation sheep red blood cell, a kind of like this composite particles had both possessed the characteristic of indication particle, possess the characteristic of solid phase carrier again and can be prepared to artificial type particulate antigen, can the significant prolongation storage life, under the effect of magnetic field, possess magnetic again simultaneously, can realize assembling rapidly and disperseing again, avoid centrifugal process loaded down with trivial details in traditional agglutination test, very convenient.The indicator cells that not only can be used for traditional agglutination test, the carrier of qualitative or quantitative detection in also can enzyme, chemiluminescent substance or fluorescent material mark test as the carrier of antigen-antibody screening agglutination test and antigen-antibody, wherein wrapping quilt behind the replacement solid phase carrier all carries out in sparing mutually with detection reaction, not only with low cost than normal enzyme target, can also make that reaction is more abundant, susceptibility is higher, mainly can be used for special erythrocyte blood type antibody and other soluble antigen antibody screenings or quantitatively detect test.
The above only is embodiments of the invention; be not so limit claim of the present invention; every equivalent structure or equivalent flow process conversion that utilizes description of the present invention to do; or directly or indirectly be used in other relevant technical field, all in like manner be included in the scope of patent protection of the present invention.
Claims (10)
1. a preparation method of magnetizing the hydroformylation sheep red blood cell is characterized in that, may further comprise the steps:
(1) nanometer magnetic bead preparation;
(2) hydroformylation sheep red blood cell preparation;
(3) magnetization hydroformylation sheep red blood cell preparation;
(4) magnetization hydroformylation sheep red blood cell coating protein;
(5) sample detection;
(6) result observes.
2. the preparation method of magnetization hydroformylation sheep red blood cell according to claim 1, it is characterized in that, the concrete steps of described step (1) nanometer magnetic bead preparation are: preparation iron salt solutions and ammonia spirit, in the iron salt solutions for preparing, add surfactant and stirring, add the ammonia spirit for preparing then and obtain nanometer magnetic bead.
3. the preparation method of magnetization hydroformylation sheep red blood cell according to claim 1, it is characterized in that, the concrete steps of described step (2) hydroformylation sheep red blood cell preparation are: with physiological saline sheep red blood cell washing back is diluted, add glutaraldehyde, leave standstill behind the mixing, unnecessary glutaraldehyde is removed in washing, and it is standby to make suspension.
4. the preparation method of magnetization hydroformylation sheep red blood cell according to claim 1, it is characterized in that, the concrete steps of described step (3) magnetization hydroformylation sheep red blood cell preparation are: determine the magnetization condition by preliminary experiment, hydroformylation sheep red blood cell and the vibration of nanometer magnetic bead mixing, magnetic force absorption washing then is with the red blood cell flush away that is not magnetized.
5. the preparation method of magnetization hydroformylation sheep red blood cell according to claim 1, it is characterized in that, the concrete steps of described step (4) magnetization hydroformylation sheep red blood cell coating protein are: will magnetize and leave standstill after hydroformylation sheep red blood cell and albumen mix, mix with the roller bearing instrument again, and then leave standstill washing, it is standby to make suspension.
6. the preparation method of magnetization hydroformylation sheep red blood cell according to claim 2 is characterized in that, described nanometer magnetic bead particle diameter is 10-500 nm.
7. the preparation method of magnetization hydroformylation sheep red blood cell according to claim 3 is characterized in that, the hematocrit percent concentration of described sheep red blood cell is 2-5%, and the mass percent concentration of glutaraldehyde is 1-2%, and both press the reaction volume of 1:1 than mixing.
8. the preparation method of magnetization hydroformylation sheep red blood cell according to claim 4 is characterized in that, the hematocrit of described hydroformylation sheep red blood cell and nanometer magnetic bead is than being 15:1.
9. the preparation method of magnetization hydroformylation sheep red blood cell according to claim 5 is characterized in that, the mass concentration of described albumen is 1-5 mg/mL, and the hematocrit percent concentration of magnetization hydroformylation sheep red blood cell is 20-50%, and the long-pending ratio of both bodies is 3:1.
10. the preparation method of magnetization hydroformylation sheep red blood cell according to claim 1, it is characterized in that the carrier of described magnetization hydroformylation sheep red blood cell qualitative or quantitative detection in can enzyme, chemiluminescent substance or fluorescent material mark test as the carrier of the indicator cells of agglutination test, antigen-antibody screening agglutination test and antigen-antibody.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103551569A (en) * | 2013-11-04 | 2014-02-05 | 同济大学 | Preparation method of GNPs (Gold Nano Particles) coated with BSA (Bovine Serum Albumin) |
| CN106644632A (en) * | 2016-11-17 | 2017-05-10 | 中国科学院苏州生物医学工程技术研究所 | Artificial substrate for single-layer cell tiling |
| CN114414795A (en) * | 2022-01-18 | 2022-04-29 | 迪佰(厦门)生物科技有限公司 | Method for manufacturing microspheres and application |
Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0156537A2 (en) * | 1984-03-02 | 1985-10-02 | Board Of Regents University Of Texas System | Biological magnetic fluids |
| JPH02141667A (en) * | 1988-11-22 | 1990-05-31 | Olympus Optical Co Ltd | Particle of blood corpuscle |
| CN1621837A (en) * | 2003-11-28 | 2005-06-01 | 长春博迅生物技术有限责任公司 | Preparation of specifically indicating erythrocyte and use in micro column immunological experimental technology |
| CN1763102A (en) * | 2005-09-09 | 2006-04-26 | 李勇 | Cell membrane antigen preservation method and magnetic particle envelope human cell membrane antigen |
| CN101315386A (en) * | 2008-07-11 | 2008-12-03 | 李勇 | High-density medium solid phase antihuman globulin reagent kit |
| CN101382540A (en) * | 2008-10-15 | 2009-03-11 | 李勇 | Platelet antigen antibody detecting or cross matching solid phase anti-human globulin kit |
| CN101387648A (en) * | 2008-10-24 | 2009-03-18 | 李勇 | Erythrocyte membrane antigen magnetic ball kit and applications on blood group antibody detection |
| CN101423548A (en) * | 2008-12-12 | 2009-05-06 | 陶海静 | Preparation of duck hepatitis I type virus indirect hemagglutination diagnostic antigen and kit |
| CN101445793A (en) * | 2008-12-09 | 2009-06-03 | 中国人民解放军南京军区南京总医院 | Method for rapidly separating supernatant from human whole blood or suspended red blood cells by utilizing lyophilized preparation |
| CN101487774A (en) * | 2009-02-25 | 2009-07-22 | 李勇 | Momentary magnetizing method for erythrocyte |
| US20090269776A1 (en) * | 2005-11-03 | 2009-10-29 | Yves Barbreau | Magnetic Immunodiagnostic Method for the Demonstration of Antibody/Antigen Complexes especially of blood groups |
| CN101711363A (en) * | 2007-06-08 | 2010-05-19 | 博瑞巴斯德公司 | The multiple analysis of blood sample |
| CN101893628A (en) * | 2009-12-30 | 2010-11-24 | 中国疾病预防控制中心寄生虫病预防控制所 | Kit for detecting circulating antigen indirect hemagglutination of schistosomiasis and manufacturing method thereof |
| WO2012010666A1 (en) * | 2010-07-21 | 2012-01-26 | Diagast | Magnetic immunodiagnostic methods and kits for the demonstration of antibody/antigen complexes in erythrocyte blood grouping and phenotyping |
| CN102703386A (en) * | 2012-06-01 | 2012-10-03 | 武汉生物制品研究所有限责任公司 | Hydroformylated fresh goose red blood cells and application of hydroformylated fresh goose red blood cells to hemagglutination (HA) test for acellular pertussis |
-
2013
- 2013-07-19 CN CN201310305454.5A patent/CN103344755B/en active Active
Patent Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0156537A2 (en) * | 1984-03-02 | 1985-10-02 | Board Of Regents University Of Texas System | Biological magnetic fluids |
| JPH02141667A (en) * | 1988-11-22 | 1990-05-31 | Olympus Optical Co Ltd | Particle of blood corpuscle |
| CN1621837A (en) * | 2003-11-28 | 2005-06-01 | 长春博迅生物技术有限责任公司 | Preparation of specifically indicating erythrocyte and use in micro column immunological experimental technology |
| CN1763102A (en) * | 2005-09-09 | 2006-04-26 | 李勇 | Cell membrane antigen preservation method and magnetic particle envelope human cell membrane antigen |
| US20090269776A1 (en) * | 2005-11-03 | 2009-10-29 | Yves Barbreau | Magnetic Immunodiagnostic Method for the Demonstration of Antibody/Antigen Complexes especially of blood groups |
| CN101711363A (en) * | 2007-06-08 | 2010-05-19 | 博瑞巴斯德公司 | The multiple analysis of blood sample |
| CN101315386A (en) * | 2008-07-11 | 2008-12-03 | 李勇 | High-density medium solid phase antihuman globulin reagent kit |
| CN101382540A (en) * | 2008-10-15 | 2009-03-11 | 李勇 | Platelet antigen antibody detecting or cross matching solid phase anti-human globulin kit |
| CN101387648A (en) * | 2008-10-24 | 2009-03-18 | 李勇 | Erythrocyte membrane antigen magnetic ball kit and applications on blood group antibody detection |
| CN101445793A (en) * | 2008-12-09 | 2009-06-03 | 中国人民解放军南京军区南京总医院 | Method for rapidly separating supernatant from human whole blood or suspended red blood cells by utilizing lyophilized preparation |
| CN101423548A (en) * | 2008-12-12 | 2009-05-06 | 陶海静 | Preparation of duck hepatitis I type virus indirect hemagglutination diagnostic antigen and kit |
| CN101487774A (en) * | 2009-02-25 | 2009-07-22 | 李勇 | Momentary magnetizing method for erythrocyte |
| CN101893628A (en) * | 2009-12-30 | 2010-11-24 | 中国疾病预防控制中心寄生虫病预防控制所 | Kit for detecting circulating antigen indirect hemagglutination of schistosomiasis and manufacturing method thereof |
| WO2012010666A1 (en) * | 2010-07-21 | 2012-01-26 | Diagast | Magnetic immunodiagnostic methods and kits for the demonstration of antibody/antigen complexes in erythrocyte blood grouping and phenotyping |
| CN102703386A (en) * | 2012-06-01 | 2012-10-03 | 武汉生物制品研究所有限责任公司 | Hydroformylated fresh goose red blood cells and application of hydroformylated fresh goose red blood cells to hemagglutination (HA) test for acellular pertussis |
Non-Patent Citations (2)
| Title |
|---|
| 李岩等: "红细胞膜免疫磁珠检测成分血中抗Ａ／抗Ｂ的研究", 《中国实验诊断学》, vol. 16, no. 8, 31 August 2012 (2012-08-31) * |
| 苏凯等: "红细胞瞬间磁化试剂的制备与应用", 《中国生物制品学杂志》, vol. 20, no. 8, 31 August 2007 (2007-08-31) * |
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| CN103551569A (en) * | 2013-11-04 | 2014-02-05 | 同济大学 | Preparation method of GNPs (Gold Nano Particles) coated with BSA (Bovine Serum Albumin) |
| CN103551569B (en) * | 2013-11-04 | 2015-08-19 | 同济大学 | The preparation method of the coated golden nanometer particle of bovine serum albumin(BSA) |
| CN106644632A (en) * | 2016-11-17 | 2017-05-10 | 中国科学院苏州生物医学工程技术研究所 | Artificial substrate for single-layer cell tiling |
| CN106644632B (en) * | 2016-11-17 | 2019-08-27 | 中国科学院苏州生物医学工程技术研究所 | Artificial substratum for cell monolayer tiling |
| CN114414795A (en) * | 2022-01-18 | 2022-04-29 | 迪佰(厦门)生物科技有限公司 | Method for manufacturing microspheres and application |
| CN114414795B (en) * | 2022-01-18 | 2022-09-23 | 迪佰(厦门)生物科技有限公司 | Method for manufacturing microspheres and application |
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