CN103344755B - Method for preparing magnetized and hydroformyled sheep red blood cell - Google Patents
Method for preparing magnetized and hydroformyled sheep red blood cell Download PDFInfo
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- CN103344755B CN103344755B CN201310305454.5A CN201310305454A CN103344755B CN 103344755 B CN103344755 B CN 103344755B CN 201310305454 A CN201310305454 A CN 201310305454A CN 103344755 B CN103344755 B CN 103344755B
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Abstract
The invention discloses a method for preparing a magnetized and hydroformyled sheep red blood cell. The method comprises the following steps of: preparing nano magnetic beads; preparing a hydroformyled sheep red blood cell; preparing a magnetized and hydroformyled sheep red blood cell; coating protein on the magnetized and hydroformyled sheep red blood cell; detecting a sample; and observing the result. The prepared magnetized and hydroformyled sheep red blood cell can be used as an indicator cell of an agglutination test, a carrier, enzyme and a chemiluminescence matter of an antigen antibody agglutination test, or a carrier detected in a fluorescent material marking test. The magnetized and hydroformyled sheep red blood cell prepared by the method is long in retention period, simultaneously has paramagnetism, can achieve rapid aggregation and redispersion, avoids complicated centrifuge processes in the traditional agglutination test, and can be used for preparing artificial granular antigen antibodies with uniform sizes; coating and detection reaction are carried out in a uniform phase after a solid-phase carrier is replaced; and the method is full in action, high in sensitivity, low in cost than an elisa plate, and is mainly applied to a screening or quantitative detection test of a special red blood cell antibody and other soluble antigen antibodies.
Description
Technical field
The present invention relates to biological technical field, particularly relate to a kind of preparation method of magnetizing hydroformylation sheep red blood cell.
Background technology
Agglutination reaction is a kind of serological reaction, process is that particulate antigen (complete pathogenic micro-organism or red corpuscle etc.) is combined with corresponding antibodies, having under ionogen existent condition, through certain hour, occur macroscopic aggegation fritter, agglutination reaction can be divided into direct agglutination reaction and indirect agglutination two class.
The direct agglutination reaction agglutination phenomenon that to be particulate state antigen (as bacterium, red corpuscle etc.) with corresponding antibodies be directly combined occurs, is mainly divided into slide method and test tube method.Slide method is a kind of qualitative test method, can detect unknown antigen by known antibodies.Test tube method is a kind of classical way of quantitative test, can detect the content be subject to or without certain antibody and antibody in inspection serum, be used for assisting clinical diagnosis or supplying epidemiological investigation by known antigens.
Indirect agglutination soluble antigen (or antibody) is first adsorbed in a kind ofly to have nothing to do with immunity, a certain size the surface of granular carrier, make people's drum particulate antigen, then act on corresponding antibodies (or antigen), under the suitable condition existed there being dielectric medium, aggegation can be there is.The microballoon being used as carrier can with natural particulate materials; as red corpuscle, the activated carbon granule or aluminum silicate particles etc. of people's (O type) and animal (sheep, rabbit etc.); also can make, as polystyrene latex microspheres etc. with synthetic or natural macromolecular material.Because carrier granule increases the reaction area of soluble antigen, when after the antigen on particle and micro-antibodies, be just enough to occur macroscopic reaction, susceptibility is more much higher than direct agglutination reaction.
Granule type antigen in agglutination reaction, use fresh cells or bacterium, all exist and should not preserve for a long time, and the defect such as complex operation, although agglutination test detected result is more directly perceived, but there is the problem of the low and poor accuracy of sensitivity, and experimental result not easily stdn, this to use in the application of the immunology detection of conventional antigen antibody reaction agglutination reaction and causes certain obstacle.Therefore, agglutination test basis develops the detection techniques such as enzyme, chemoluminescence or fluorescent mark, utilize people's drum particulate matter to increase the reaction area of soluble antigen and responsive detection means, make soluble antigen antibody test more accurately, fast.Current employing synthetic or natural macromolecular material make some microballoons, for the detection of soluble antigen antibody, but such microballoon building-up process is more loaded down with trivial details, requirement for experiment condition is harsh, buy finished product price high, and there is size particles heterogeneity, and the shortcoming such as protein labeling efficiency is low, so be not widely used clinically so far.
Summary of the invention
The technical problem that the present invention mainly solves is to provide a kind of preparation method of magnetizing hydroformylation sheep red blood cell, composite particles prepared by the present invention had both possessed the characteristic of instruction particle, possess again the characteristic of solid phase carrier and people's drum particulate antigen antibody can be made into, preservation period is longer, again there is paramagnetism simultaneously, can realize assembling rapidly and redispersion, avoid centrifugal process loaded down with trivial details in traditional agglutination test, very convenient, it can be used for the indicator cells as traditional agglutination test, the carrier of antigen-antibody screening agglutination test and the enzyme of antigen-antibody, the carrier of qualitative or detection by quantitative in chemiluminescent substance or fluorescent substance mark test, this carrier can replace solid phase carrier and be prepared into the homogeneous people's drum particulate antigen antibody of size, wherein bag quilt and detection reaction all even mutually in carry out, not only with low cost than normal enzyme target, more make reaction more abundant, susceptibility is higher.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is: provide a kind of preparation method of magnetizing hydroformylation sheep red blood cell, comprise the following steps:
(1) nanometer magnetic bead preparation;
(2) hydroformylation sheep red blood cell preparation;
(3) preparation of hydroformylation sheep red blood cell is magnetized;
(4) hydroformylation sheep red blood cell coating protein is magnetized;
(5) sample detection;
(6) result is observed.
In a preferred embodiment of the present invention, concrete steps prepared by described step (1) nanometer magnetic bead are: preparation iron salt solutions and ammonia soln, in the iron salt solutions prepared, add tensio-active agent and stir, then adding the ammonia soln prepared and obtain nanometer magnetic bead.
In a preferred embodiment of the present invention, concrete steps prepared by described step (2) hydroformylation sheep red blood cell are: diluted by sheep red blood cell with physiological saline, add glutaraldehyde, leave standstill after mixing, and the unnecessary glutaraldehyde of washing removing, makes suspension for subsequent use.
In a preferred embodiment of the present invention, concrete steps prepared by described step (3) magnetization hydroformylation sheep red blood cell are: by preliminary experiment determination magnetization condition, hydroformylation sheep red blood cell and nanometer magnetic bead mixing vibration, then magnetic-adsorption washing, washes away the red corpuscle be not magnetized.
In a preferred embodiment of the present invention, the concrete steps of described step (4) magnetization hydroformylation sheep red blood cell coating protein for: leave standstill after will magnetize hydroformylation sheep red blood cell and albumen mixing, then mix with roller bearing instrument, and then standingly to wash, make suspension for subsequent use.
In a preferred embodiment of the present invention, described nanometer magnetic bead particle diameter is 10-500 nm.
In a preferred embodiment of the present invention, the hematocrit percentage concentration of described sheep red blood cell is 2-5%, and the mass percent concentration of glutaraldehyde is 1-2%, and both press the reaction volume of 1:1 than mixing.
In a preferred embodiment of the present invention, the hematocrit of described hydroformylation sheep red blood cell and nanometer magnetic bead is than being 15:1.
In a preferred embodiment of the present invention, the mass concentration of described albumen is 1-5 mg/mL, and the hematocrit percentage concentration of magnetization hydroformylation sheep red blood cell is 20-50%, and the long-pending ratio of both bodies is 3:1.
In a preferred embodiment of the present invention, described magnetization hydroformylation sheep red blood cell can as carrier that is qualitative in the enzyme of the carrier of the indicator cells of agglutination test, antigen-antibody screening agglutination test and antigen-antibody, chemiluminescent substance or fluorescent substance mark test or detection by quantitative.
The invention has the beneficial effects as follows:
1, the sheep red blood cell wide material sources that the present invention relates to, with low cost, size is homogeneous, sheep red blood cell after hydroformylation not easily haemolysis, the time of more than 3 months can be preserved, because of but one well indicates particle, have in traditional agglutination test and apply very widely.
2, nanometer magnetic bead is incorporated into hydroformylation sheep red blood cell surface by the present invention innovatively, form magnetization hydroformylation sheep red blood cell, a kind of like this composite particles had both possessed the characteristic of instruction particle, possess again the characteristic of solid phase carrier and people's drum particulate antigen can be prepared to, preservation period is longer, has paramagnetism again simultaneously, can realize assembling rapidly and redispersion, avoid centrifugal process loaded down with trivial details in traditional agglutination test, very convenient.
3, the indicator cells of traditional agglutination test can not only be used for, also can as the carrier of antigen-antibody screening agglutination test and antigen-antibody enzyme, chemiluminescent substance or fluorescent substance mark test in the carrier of qualitative or detection by quantitative, replace wherein wrapping after solid phase carrier quilt and detection reaction all even mutually in carry out, not only with low cost than normal enzyme target, reaction can also be made more abundant, susceptibility is higher, mainly may be used for special erythrocyte blood type antibody and other soluble antigen antibody screenings or detection by quantitative and tests.
Accompanying drawing explanation
In order to be illustrated more clearly in the technical scheme in the embodiment of the present invention, below the accompanying drawing used required in describing embodiment is briefly described, apparently, accompanying drawing in the following describes is only some embodiments of the present invention, for those of ordinary skill in the art, under the prerequisite not paying creative work, other accompanying drawing can also be obtained according to these accompanying drawings, wherein:
Fig. 1 is the figure of the scanning electron microscope of the magnetization hydroformylation sheep red blood cell that preparation method one preferred embodiment that hydroformylation sheep red blood cell is magnetized in the present invention obtains.
Embodiment
Be clearly and completely described to the technical scheme in the embodiment of the present invention below, obviously, described embodiment is only a part of embodiment of the present invention, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making other embodiments all obtained under creative work prerequisite, belong to the scope of protection of the invention.
Embodiment one:
There is provided a kind of application hydroformylation magnetization sheep red blood cell to detect agglutination test model to soluble antibody, detect other specific antibodies, the specific antigens bag of its correspondence is magnetized on sheep red blood cell by hydroformylation, can apply and antibody test.Comprise the steps:
1, the preparation of nanometer magnetic bead
0.85g FeCl
36H
2o and 0.30g FeCl
24H
2o is dissolved in 200 mL distilled water under nitrogen protection.Add proper amount of surfactant, under strong stirring, 1.5 mol/L ammonia solns are slowly joined in above-mentioned solution, when the pH of solution is increased to 6 ~ 7, in solution, produce a large amount of black Fe
3o
4particle; Continue to add ammoniacal liquor to pH=8, make hydrolysis complete.Ageing 0.5 h at 80 DEG C.The solid generated is separated, with distillation washing 3 times, is then dispersed under ultrasonication in 100 mL distilled water, obtains Fe
3o
4colloidal solution collect for subsequent use.
2, sheep red blood cell preparation
By 10 mL fresh sheep blood centrifugal 10 min under 1500 rpm, collect red corpuscle, then use brine 3 times, make 2 mL red cell suspensions for subsequent use.
3, glutaraldehyde configuration
2% concentration glutaraldehyde 10 mL is configured with physiological saline, for subsequent use.
4, sheep red blood cell hydroformylation
(1) with physiological saline, sheep red blood cell is diluted to 5% concentration, gets 5 mL, add isopyknic 2% concentration glutaraldehyde, gently after mixing, 4 DEG C leave standstill 30 min;
(2) use brine 3 times, wash away unnecessary glutaraldehyde, make suspension with physiological saline for subsequent use.
5, hydroformylation sheep red blood cell magnetization
(1) by preliminary experiment determination magnetization condition, Hydroformylated red blood cell and magnetic bead hematocrit, than being 15:1, respectively get 1 mL mixing vibration 2min;
(2) magnetic-adsorption washing, washes away the red corpuscle be not magnetized.
6, hydroformylation magnetization sheep red blood cell bag is by blood group polypeptide antigen
(1) mass concentration of blood group polypeptide antigen is 2 mg/mL, is the PBS dilution of 7.2 by the pH value of 0.15M;
(2) pH value of magnetizing hydroformylation sheep red blood cell 0.15M be 7.2 PBS replace and be diluted to mass percent concentration 50%;
(3) magnetize hydroformylation sheep red blood cell and blood group polypeptide antigen volume ratio 3:1, both mixing, room temperature leaves standstill 30 min;
(4) 5 h are mixed with roller bearing instrument, rotating speed 100 r/min;
(5) room temperature washs 3 times with 0.15 M PH7.2 PBS after leaving standstill 30 min, makes suspension for subsequent use.
7, sample detection
(1) bag is magnetized sheep erythrocyte suspension 100 μ l in 96 enzyme plate holes, hole by the hydroformylation of blood group polypeptide antigen, add each 50 μ l of normal people's sample/negative sample/dummy, vibrate 1 min;
After 30 min are hatched in (2) 37 DEG C of water-baths, period vibration mixing, with program control magnetic oscillator absorption washing 3 times;
(3) goat-anti people IgM is added, after 30 min are hatched in 37 DEG C of water-baths, vibration mixing observations;
(4) there is agglutination phenomenon in positive findings, and feminine gender and blank are then without agglutination phenomenon.
8, detection and result interpretation.
Embodiment two:
There is provided a kind of application hydroformylation magnetization sheep red blood cell for antibody fluorescence detection experiment model, specific antigens bag is magnetized on sheep red blood cell by hydroformylation, for antibody test.Comprise the steps:
1, nanometer magnetic bead preparation
0.85g FeCl
36H
2o and 0.30g FeCl
24H
2o is dissolved in 200 mL distilled water under nitrogen protection.Add proper amount of surfactant, under strong stirring, 1.5 mol/L ammonia solns are slowly joined in above-mentioned solution, when the pH of solution is increased to 6 ~ 7, in solution, produce a large amount of black Fe
3o
4particle; Continue to add ammoniacal liquor to pH=8, make hydrolysis complete.Ageing 0.5 h at 80 DEG C.The solid generated is separated, with distillation washing 3 times, is then dispersed under ultrasonication in 100 mL distilled water, obtains Fe
3o
4colloidal solution collect for subsequent use.
2, sheep red blood cell preparation
By 10 mL fresh sheep blood at centrifugal 10 min of 1500 rpm, collect red corpuscle, then use brine 3 times, make 2 mL red cell suspensions for subsequent use.
3, glutaraldehyde configuration
2% concentration glutaraldehyde 10 mL is configured with physiological saline, for subsequent use.
4, sheep red blood cell hydroformylation
(1) with physiological saline, sheep red blood cell is diluted to 5% concentration, gets 5 mL, add isopyknic 2% concentration glutaraldehyde, gently after mixing, 4 DEG C leave standstill 30 min;
(2) use brine 3 times, wash away unnecessary glutaraldehyde, make suspension with physiological saline for subsequent use.
5, hydroformylation sheep red blood cell magnetization
(1) by preliminary experiment determination magnetization condition, Hydroformylated red blood cell and nanometer magnetic bead hematocrit, than being 15:1, respectively get 1 mL mixing vibration 2min,
(2) magnetic-adsorption washing, washes away the red corpuscle be not magnetized.
6, hydroformylation magnetization sheep red blood cell bag is by human IgG
(1) mass concentration of IgG is 2mg/mL, is the PBS dilution of 7.2 by the pH value of 0.15M;
(2) pH value of hydroformylation magnetization sheep red blood cell 0.15M be 7.2 PBS to replace and be diluted to mass percent concentration be 50% dense;
(3) hydroformylation magnetization sheep red blood cell and IgG volume ratio 3:1, both mixing, room temperature leaves standstill 30 min;
(4) 5 h are mixed with roller bearing instrument, rotating speed 100rpm;
(5) wash 3 times with the PBS that the pH value of 0.15M is 7.2 after room temperature leaves standstill 30 min, make suspension for subsequent use.
7, sample detection
(1) bag is magnetized sheep erythrocyte suspension in 96 enzyme plate holes, hole by the hydroformylation of human IgG;
(2) add the goat anti-human igg of FITC mark, 30 min are hatched in 37 DEG C of water-baths, wash 2 times;
(3) flow cytometer or fluorescence detector read fluorescent signal value.
The invention has the beneficial effects as follows: the sheep red blood cell wide material sources that the present invention relates to, with low cost, size is homogeneous, sheep red blood cell after hydroformylation not easily haemolysis, the time of more than 3 months can be preserved, because of but one well indicates particle, have in traditional agglutination test and apply very widely.Nanometer magnetic bead is incorporated into hydroformylation sheep red blood cell surface by the present invention innovatively, form magnetization hydroformylation sheep red blood cell, a kind of like this composite particles had both possessed the characteristic of instruction particle, possess again the characteristic of solid phase carrier and people's drum particulate antigen can be prepared to, can significant prolongation preservation period, under the action of a magnetic field, possess magnetic again simultaneously, can realize assembling rapidly and redispersion, avoid centrifugal process loaded down with trivial details in traditional agglutination test, very convenient.The indicator cells in traditional agglutination test can not only be used for, also can as carrier that is qualitative in the enzyme of the carrier of antigen-antibody screening agglutination test and antigen-antibody, chemiluminescent substance or fluorescent substance mark test or detection by quantitative, replace wherein wrapping after solid phase carrier quilt and detection reaction all even mutually in carry out, not only with low cost than normal enzyme target, reaction can also be made more abundant, susceptibility is higher, mainly may be used for special erythrocyte blood type antibody and other soluble antigen antibody screenings or detection by quantitative and tests.
The foregoing is only embodiments of the invention; not thereby the scope of the claims of the present invention is limited; every utilize description of the present invention to do equivalent structure or equivalent flow process conversion; or be directly or indirectly used in other relevant technical field, be all in like manner included in scope of patent protection of the present invention.
Claims (9)
1. magnetize a preparation method for hydroformylation sheep red blood cell, it is characterized in that, comprise the following steps:
(1) nanometer magnetic bead preparation;
(2) hydroformylation sheep red blood cell preparation;
(3) preparation of hydroformylation sheep red blood cell is magnetized;
(4) hydroformylation sheep red blood cell coating protein is magnetized;
(5) sample detection;
(6) result is observed, and wherein said magnetization hydroformylation sheep red blood cell can as carrier that is qualitative in the enzyme of the carrier of the indicator cells of agglutination test, antigen-antibody screening agglutination test and antigen-antibody, chemiluminescent substance or fluorescent substance mark test or detection by quantitative.
2. the preparation method of magnetization hydroformylation sheep red blood cell according to claim 1, it is characterized in that, concrete steps prepared by described step (1) nanometer magnetic bead are: preparation iron salt solutions and ammonia soln, in the iron salt solutions prepared, add tensio-active agent and stir, then adding the ammonia soln prepared and obtain nanometer magnetic bead.
3. the preparation method of magnetization hydroformylation sheep red blood cell according to claim 1, it is characterized in that, concrete steps prepared by described step (2) hydroformylation sheep red blood cell are: with physiological saline by dilution after sheep red blood cell washing, add glutaraldehyde, leave standstill after mixing, the unnecessary glutaraldehyde of washing removing, makes suspension for subsequent use.
4. the preparation method of magnetization hydroformylation sheep red blood cell according to claim 1, it is characterized in that, concrete steps prepared by described step (3) magnetization hydroformylation sheep red blood cell are: by preliminary experiment determination magnetization condition, hydroformylation sheep red blood cell and nanometer magnetic bead mixing vibration, then magnetic-adsorption washing, washes away the red corpuscle be not magnetized.
5. the preparation method of magnetization hydroformylation sheep red blood cell according to claim 1, it is characterized in that, the concrete steps of described step (4) magnetization hydroformylation sheep red blood cell coating protein for: will magnetize hydroformylation sheep red blood cell and albumen mixing is standing afterwards, again with the mixing of roller bearing instrument, and then leave standstill washing, make suspension for subsequent use.
6. according to the preparation method of the magnetization hydroformylation sheep red blood cell described in claim 2, it is characterized in that, described nanometer magnetic bead particle diameter is 10-500 nm.
7. according to the preparation method of the magnetization hydroformylation sheep red blood cell described in claim 3, it is characterized in that, the hematocrit percentage concentration of described sheep red blood cell is 2-5%, and the mass percent concentration of glutaraldehyde is 1-2%, and both press the reaction volume of 1:1 than mixing.
8. according to the preparation method of the magnetization hydroformylation sheep red blood cell described in claim 4, it is characterized in that, the hematocrit of described hydroformylation sheep red blood cell and nanometer magnetic bead is than being 15:1.
9. according to the preparation method of the magnetization hydroformylation sheep red blood cell described in claim 5, it is characterized in that, the mass concentration of described albumen is 1-5 mg/mL, and the hematocrit percentage concentration of magnetization hydroformylation sheep red blood cell is 20-50%, and the long-pending ratio of both bodies is 3:1.
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| CN103551569B (en) * | 2013-11-04 | 2015-08-19 | 同济大学 | The preparation method of the coated golden nanometer particle of bovine serum albumin(BSA) |
| CN106644632B (en) * | 2016-11-17 | 2019-08-27 | 中国科学院苏州生物医学工程技术研究所 | Artificial substratum for cell monolayer tiling |
| CN114414795B (en) * | 2022-01-18 | 2022-09-23 | 迪佰(厦门)生物科技有限公司 | Method for manufacturing microspheres and application |
Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0156537A2 (en) * | 1984-03-02 | 1985-10-02 | Board Of Regents University Of Texas System | Biological magnetic fluids |
| JPH02141667A (en) * | 1988-11-22 | 1990-05-31 | Olympus Optical Co Ltd | Particle of blood corpuscle |
| CN1621837A (en) * | 2003-11-28 | 2005-06-01 | 长春博迅生物技术有限责任公司 | Preparation of specifically indicating erythrocyte and use in micro column immunological experimental technology |
| CN1763102A (en) * | 2005-09-09 | 2006-04-26 | 李勇 | Cell membrane antigen preservation method and magnetic particle envelope human cell membrane antigen |
| CN101315386A (en) * | 2008-07-11 | 2008-12-03 | 李勇 | High-density medium solid phase antihuman globulin reagent kit |
| CN101382540A (en) * | 2008-10-15 | 2009-03-11 | 李勇 | Platelet antigen antibody detecting or cross matching solid phase anti-human globulin kit |
| CN101387648A (en) * | 2008-10-24 | 2009-03-18 | 李勇 | Erythrocyte membrane antigen magnetic ball kit and applications on blood group antibody detection |
| CN101423548A (en) * | 2008-12-12 | 2009-05-06 | 陶海静 | Preparation of duck hepatitis I type virus indirect hemagglutination diagnostic antigen and kit |
| CN101445793A (en) * | 2008-12-09 | 2009-06-03 | 中国人民解放军南京军区南京总医院 | Method for rapidly separating supernatant from human whole blood or suspended red blood cells by utilizing lyophilized preparation |
| CN101487774A (en) * | 2009-02-25 | 2009-07-22 | 李勇 | Momentary magnetizing method for erythrocyte |
| CN101711363A (en) * | 2007-06-08 | 2010-05-19 | 博瑞巴斯德公司 | The multiple analysis of blood sample |
| CN101893628A (en) * | 2009-12-30 | 2010-11-24 | 中国疾病预防控制中心寄生虫病预防控制所 | Kit for detecting circulating antigen indirect hemagglutination of schistosomiasis and manufacturing method thereof |
| WO2012010666A1 (en) * | 2010-07-21 | 2012-01-26 | Diagast | Magnetic immunodiagnostic methods and kits for the demonstration of antibody/antigen complexes in erythrocyte blood grouping and phenotyping |
| CN102703386A (en) * | 2012-06-01 | 2012-10-03 | 武汉生物制品研究所有限责任公司 | Hydroformylated fresh goose red blood cells and application of hydroformylated fresh goose red blood cells to hemagglutination (HA) test for acellular pertussis |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2892820B1 (en) * | 2005-11-03 | 2008-02-01 | Diagast Soc Par Actions Simpli | MAGNETIC IMMUNODIAGNOSTIC METHOD FOR THE EVIDENCE OF ANTIBODY / ANTIGEN COMPLEX, PARTICULARLY BLOOD GROUP |
-
2013
- 2013-07-19 CN CN201310305454.5A patent/CN103344755B/en active Active
Patent Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0156537A2 (en) * | 1984-03-02 | 1985-10-02 | Board Of Regents University Of Texas System | Biological magnetic fluids |
| JPH02141667A (en) * | 1988-11-22 | 1990-05-31 | Olympus Optical Co Ltd | Particle of blood corpuscle |
| CN1621837A (en) * | 2003-11-28 | 2005-06-01 | 长春博迅生物技术有限责任公司 | Preparation of specifically indicating erythrocyte and use in micro column immunological experimental technology |
| CN1763102A (en) * | 2005-09-09 | 2006-04-26 | 李勇 | Cell membrane antigen preservation method and magnetic particle envelope human cell membrane antigen |
| CN101711363A (en) * | 2007-06-08 | 2010-05-19 | 博瑞巴斯德公司 | The multiple analysis of blood sample |
| CN101315386A (en) * | 2008-07-11 | 2008-12-03 | 李勇 | High-density medium solid phase antihuman globulin reagent kit |
| CN101382540A (en) * | 2008-10-15 | 2009-03-11 | 李勇 | Platelet antigen antibody detecting or cross matching solid phase anti-human globulin kit |
| CN101387648A (en) * | 2008-10-24 | 2009-03-18 | 李勇 | Erythrocyte membrane antigen magnetic ball kit and applications on blood group antibody detection |
| CN101445793A (en) * | 2008-12-09 | 2009-06-03 | 中国人民解放军南京军区南京总医院 | Method for rapidly separating supernatant from human whole blood or suspended red blood cells by utilizing lyophilized preparation |
| CN101423548A (en) * | 2008-12-12 | 2009-05-06 | 陶海静 | Preparation of duck hepatitis I type virus indirect hemagglutination diagnostic antigen and kit |
| CN101487774A (en) * | 2009-02-25 | 2009-07-22 | 李勇 | Momentary magnetizing method for erythrocyte |
| CN101893628A (en) * | 2009-12-30 | 2010-11-24 | 中国疾病预防控制中心寄生虫病预防控制所 | Kit for detecting circulating antigen indirect hemagglutination of schistosomiasis and manufacturing method thereof |
| WO2012010666A1 (en) * | 2010-07-21 | 2012-01-26 | Diagast | Magnetic immunodiagnostic methods and kits for the demonstration of antibody/antigen complexes in erythrocyte blood grouping and phenotyping |
| CN102703386A (en) * | 2012-06-01 | 2012-10-03 | 武汉生物制品研究所有限责任公司 | Hydroformylated fresh goose red blood cells and application of hydroformylated fresh goose red blood cells to hemagglutination (HA) test for acellular pertussis |
Non-Patent Citations (2)
| Title |
|---|
| 李岩等.红细胞膜免疫磁珠检测成分血中抗A/抗B的研究.《中国实验诊断学》.2012,第16卷(第8期), * |
| 红细胞瞬间磁化试剂的制备与应用;苏凯等;《中国生物制品学杂志》;20070831;第20卷(第8期);596-599 * |
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