CN214750354U - Novel coronavirus antigen and antibody combined intelligent detection device - Google Patents
Novel coronavirus antigen and antibody combined intelligent detection device Download PDFInfo
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- CN214750354U CN214750354U CN202120017150.9U CN202120017150U CN214750354U CN 214750354 U CN214750354 U CN 214750354U CN 202120017150 U CN202120017150 U CN 202120017150U CN 214750354 U CN214750354 U CN 214750354U
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Abstract
The utility model relates to an immunodiagnosis technical field overcomes the single and low problem of sensitivity of current new crown detection device detection mode, discloses a novel coronavirus antigen, antibody joint intelligent detection device, detection device includes plastic casing, lower plastic casing, it is equipped with antibody detection group and antigen detection group respectively to go up the plastic casing, and antibody detection group includes antibody detection observation window and antibody detection application of sample hole, and antigen detection group includes antigen detection observation window and antigen detection application of sample hole. This joint diagnosis reagent detection cycle is short, and the testing process is simple, and this product adopts the mode that antigen antibody jointly detected, and the detection window period can directly be shortened to antigen product, and the result shows directly perceived, accurate, and the specificity is strong, and antibody product will help distinguishing recent and past infection, and code information is swept to on-the-spot APP, and patient's information can in time be fed back to relevant big data platform of disease control, accomplishes early discovery, early control.
Description
Technical Field
The utility model belongs to the technical field of immunodiagnosis technique and specifically relates to a novel coronavirus antigen, antibody joint intelligent detection device is related to.
Background
The novel coronavirus pneumonia (new coronavirus pneumonia for short, COVID-19 for short) is an acute respiratory infectious disease caused by infection of the novel coronavirus (new coronavirus for short, SARS-CoV-2 for short). The novel coronavirus is a new strain of coronavirus that has not been previously discovered in humans. The initial symptoms of the patients are fever, hypodynamia and dry cough, and a few patients are accompanied with upper respiratory tract and digestive tract symptoms such as nasal obstruction, watery nasal discharge, diarrhea and the like. Severe cases often develop dyspnea after 1 week, and severe cases rapidly progress to acute respiratory distress syndrome, septic shock, uncorrectable metabolic acidosis and hemorrhagic coagulation dysfunction, multiple organ failure, and the like. At present, effective antiviral drugs aiming at the pathogen are lacked, and mainly used for isolation treatment and symptomatic support treatment.
The current diagnostic methods for SARS-CoV-2 mainly include nucleic acid detection, antibody detection and antigen detection. The nucleic acid detection is currently used as a 'gold standard' for detecting the new coronavirus (SARS-CoV-2), primers and fluorescent probes are designed in a highly conserved region of a novel coronavirus ORF1ab/N gene sequence through fluorescence-PCR, and the virus RNA in a suspected novel coronavirus (SARS-CoV-2) infected patient specimen is amplified and detected through a full-automatic fluorescence PCR instrument, so that the qualitative detection of the novel coronavirus (SARS-CoV-2) RNA is realized, but the operation is complex, a special laboratory and professional training personnel are needed, the detection period is longer, and the labor cost is high. The reagent for detecting the neocorona antigen and the antibody is simple to operate, does not need special instruments and equipment, and can finish diagnosis in a short time. The combined diagnosis can be used as the differential diagnosis of the infection period, the infection is early, the patient does not have immune response, the antibody can not be detected in vivo or the detection rate of the antibody is low, but the antigen concentration is higher; in the middle and later stages after infection, the virus is possibly inhibited to reduce the concentration, and the in-vivo antibody (mainly IgG) is maintained at a certain titer and can be detected, so that the detection rate of the new coronary pneumonia is greatly improved.
The current common methods for detecting the new crown antibody comprise an enzyme-linked immunosorbent assay (ELISA), a chemiluminescence immunoassay and a colloidal gold immunochromatography; the detection of the new crown antigen is mainly focused on immunochromatography. ELISA is a high-sensitivity immunological experiment technology combining antigen, antibody specific reaction and high-efficiency catalytic action of enzyme on a substrate. The detection method has the advantages of high sensitivity, low carrier standardization difficulty, low detection speed, easy pollution and more complicated steps. The chemiluminescence immunoassay combines a high-sensitivity chemiluminescence assay technology with a high-specificity immunoreaction and is used for detecting various antigens, antibodies, hormones and the like. The method has sensitivity higher than that of ELISA, has the characteristics of strong specificity, wide linear range, stable result, simplified operation and the like, and is widely applied to detection of clinical specimens. However, the method has high requirements on equipment, operation and use environment, and a matched chemiluminescence instrument is required during use. The immunochromatography takes colloidal gold particles as a tracer marker, is a novel immunolabeling technology applied to antigen-antibody detection, can obtain a detection result only within 15 min through visual observation, breaks through the limitation of the existing detection technology on personnel and places, shortens the detection time, is convenient and quick to operate, and is widely applied to basic medical units and field detection.
At present, the detection of new coronavirus is emphasized and cannot be replaced mutually, so that the development of differential diagnosis capable of simultaneously detecting antigen and antibody is crucial, the antigen and antibody combined detection can effectively shorten the detection window period, improve the positive detection rate and provide double guarantee for various possible risk groups.
Patent No. CN202010196320.4, disclosing "Combined diagnosis COVID-19 and Mycoplasma pneumoniae colloidal gold immunochromatography test paper and preparation method thereof", the utility model relates to the technical field of immunodiagnosis, aiming at the blank problem of prior art COVID-19 and Mycoplasma pneumoniae antibody detection, disclosing colloidal gold immunochromatography test paper for providing combined diagnosis COVID-19 and Mycoplasma pneumoniae, specifically comprising the following preparation steps: 1) preparing a detection line T and a quality control line C; 2) preparing a colloidal gold conjugate treatment pad; 3) preparing detection test paper: and (3) preparing the water absorption pad, the sample pad and the colloidal gold conjugate treatment pad, and connecting and assembling the modified nitrocellulose membrane coated with the antibody and the PVC base plate in sequence to obtain a finished product. The prepared product can accurately detect whether the pneumonia of 2019-nCoV and mycoplasma is caused, the detection period is short, the detection process is simple, the equipment is portable, the antigen detection mode is adopted, the antigen has no incubation period, the result display is visual and accurate, the specificity is strong, the infection of new corona and pulmonary bronchi at different time can be diagnosed, and the detection efficiency is high.
The method has the disadvantages that only antibody detection is adopted, the detection mode is single, and the condition of missed detection is easy to occur.
SUMMERY OF THE UTILITY MODEL
The utility model relates to an overcome the problem that current new crown detection device detection mode is single and sensitivity is low, provide a novel coronavirus antigen, the intelligent detection device is united to the antibody, this joint diagnosis reagent detection cycle is short, the detection process is simple, and this product is the mode that adopts the antigen-antibody joint detection, the antigen product can directly shorten the detection window phase, the result shows directly perceived, it is accurate, the specificity is strong, and the antibody product will help distinguishing near term and past infection, help suspected patient's diagnosis, code information is swept to on-the-spot APP, can in time feed back patient's information to the big data platform of relevant disease control, accomplish early discovery, early control.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
the utility model provides a novel coronavirus antigen, antibody unite intelligent detection device, detection device includes plastic casing, lower plastic casing, it is equipped with antibody detection group and antigen detection group respectively to go up the plastic casing, and antibody detection group includes antibody detection observation window and antibody detection application of sample hole, and antigen detection group includes antigen detection observation window and antigen detection application of sample hole.
Preferably, an antibody detection reagent strip clamping groove and an antigen detection reagent strip clamping groove are formed in the inner side of the lower plastic shell, the antibody detection reagent strip clamping groove corresponds to the antibody detection group, and the antigen detection reagent strip clamping groove corresponds to the antigen detection group.
The upper plastic shell and the lower plastic shell are combined through the fixing protrusions and the fixing boss grooves to fix the whole detection device, the reagent strip clamping grooves are used for placing reagent strips, the reagent strips are added into the reagent strips from the sample adding holes, the test results are observed through the observation windows, the detection device can accurately detect the results of the added reagent, the detection device is small in size, simple and convenient to drip, long in validity period and high in accuracy of the detection results. The window for detecting the novel coronavirus antibody is positioned at the left side of the test plate, a sample window and a buffer window are separated, and the window and a single window for detecting the antigen at the right side are easy to distinguish, so that the wrong sample adding of an experiment is not easy to cause; the sample adding holes are located above the buffer solution windows, after sample adding, the sample is combined with the labeling pad in advance, the combined compound is transferred to the nitrocellulose membrane through the buffer solution for chromatography, and the sample and the raw materials on the labeling pad can be combined more fully through the two-window sample adding mode, so that the clinical detection rate is improved. And after the template is added with sample, the added sample cannot be influenced by the impact force of the buffer solution, so that the sample is impacted to the edge of the template, and the condition of missing detection is caused.
Preferably, the inner side of the upper plastic shell is provided with a fixing bulge, and the fixing bulge surrounds the edge of the inner side of the upper plastic shell; the inner side of the lower plastic shell is provided with a fixed boss groove which surrounds the inner side edge of the lower plastic shell, and the fixed protrusion is positioned in the fixed boss groove.
The fixed bulges and the fixed boss grooves are connected in a distributed mode along the circumferential direction, so that the circumferential distribution of the connecting force between the lower plastic shells is uniform, and the connecting effect is guaranteed.
Preferably, the antibody detection reagent strip slot and the antigen detection reagent strip slot are of the same structure.
Preferably, the antibody detection reagent strip clamping groove comprises an end flange, a side flange and a bottom support positioned between the end flange and the side flange.
Preferably, the end flanges are U-shaped, and a connecting strip is arranged between the side flanges.
The position that can fill up the contact with the sample with the test paper can be fixed to tip U type flange, fixes the reagent strip at reagent strip draw-in groove within range with the side flange combined action simultaneously, prevents that reagent strip circumferential direction from removing, influences the result of use.
Preferably, the inner side of the upper plastic shell is provided with a test paper compression column and a test paper compression block, the test paper compression column is located at two ends of the antibody detection observation window, and the test paper compression block is located between the antibody detection sampling hole and the antibody detection observation window.
The test paper pressing column is used for pressing the end part of the reagent strip to prevent the reagent strip from warping; the test paper compact heap is for compressing tightly the middle part of reagent strip, prevents the protruding perk of reagent strip interlude.
Preferably, the antibody detection reagent strip clamping groove and the antigen detection reagent strip clamping groove are respectively provided with an antibody detection reagent strip and an antigen detection reagent strip.
Preferably, the edges of the antibody detection observation window, the antibody detection sample adding hole, the antigen detection observation window and the antigen detection sample adding hole are all in an inverted horn mouth shape, and the surface of the upper plastic shell is provided with a two-dimensional code scanning area.
Dropwise add effect can be guaranteed in dropwise add sample liquid and buffer solution for the dropwise add liquid that splashes around the hole can be along falling the bell mouth border and flowing downwards, reachs smoothly and carries out relevant detection on the detection test paper strip, and the scene can adopt APP scanning two-dimensional code scanning area, and patient's information can be in time fed back to relevant big data platform of illness accuse, accomplishes early discovery, early control, and the operating efficiency is high, has promoted prevention and control efficiency.
Preferably, the antibody detection set further comprises a buffer window.
Therefore, the utility model discloses following beneficial effect has:
(1) the immunochromatography detection test paper for combined diagnosis of the COVID-19 antigen and antibody is provided, a product for detecting the new coronavirus by using the antigen and antibody detection test paper is prepared, the detection time is short, only 15-20min is needed, the requirement of field detection can be met, and the antigen and the antibody are mutually supplemented, so that the clinical omission risk can be reduced;
(2) the accuracy of directly detecting the antigen is higher than that of the detected antibody, whether the prepared antigen has the new coronavirus can be accurately detected, the antigen has no incubation period, and the detected antigen can better avoid the clinical missed detection condition caused by low antibody titer in the window period. The double detection can ensure the validity of the result;
(3) the operation is simple and convenient, and other equipment and instruments are not needed; the detection result is displayed visually, can be judged by naked eyes and is suitable for personal use; the detection efficiency is high, and the detection result is more direct; the code is swept to cooperation cell-phone APP, but on-the-spot real-time intelligent feedback testing result provides data for the epidemic prevention center, shortens the transmission time of information.
Drawings
Fig. 1 is a schematic top view of the outer side structure of the upper plastic shell of the present invention.
Fig. 2 is a schematic view of the inner side structure of the upper plastic shell of the present invention.
Fig. 3 is a schematic view of the inner side of the lower plastic shell of the present invention.
Fig. 4 is a schematic diagram of the structure of the buffer-free window of the present invention.
Fig. 5 is a plan view of the present invention.
Fig. 6 is a diagram of the detection process of the present invention.
In the figure: 1. an upper plastic shell; 2. a lower plastic shell; 3. an antibody detection observation window; 4. antibody detection wells; 5. a buffer window; 6. an antigen detection observation window; 7. antigen detection wells; 8. an antibody detection reagent strip card slot; 8.1, end flanges; 8.2, side flanges; 8.21, connecting strips; 8.3, mounting; 9. an antigen detection reagent strip slot; 10. a fixed boss groove; 11. a fixed protrusion; 12. a two-dimensional code scanning area; 13. the test paper compresses the column; 14. test paper compact heap.
Detailed Description
The present invention will be further described with reference to the accompanying drawings and the detailed description.
Example 1
In the embodiment shown in fig. 1-3, the novel coronavirus antigen and antibody combined intelligent detection device comprises an upper plastic shell 1 and a lower plastic shell 2, wherein the upper plastic shell 1 is respectively provided with an antibody detection group and an antigen detection group, the antibody detection group comprises an antibody detection observation window 3, an antibody detection sample adding hole 4 and a buffer solution window 5, and the antigen detection group comprises an antigen detection observation window 6 and an antigen detection sample adding hole 7.
An antibody detection reagent strip clamping groove 8 and an antigen detection reagent strip clamping groove 9 are formed in the inner side of the lower plastic shell 2, the antibody detection reagent strip clamping groove 8 corresponds to the antibody detection group, and the antigen detection reagent strip clamping groove 9 corresponds to the antigen detection group.
A fixing bulge 11 is arranged on the inner side of the upper plastic shell 1, and the fixing bulge 11 surrounds the edge of the inner side of the upper plastic shell 1; the inner side of the lower plastic shell 2 is provided with a fixed boss groove 10, the fixed boss groove 10 surrounds the inner side edge of the lower plastic shell 2, and the fixed protrusion 11 is positioned in the fixed boss groove 10. The antibody detection reagent strip clamping groove 8 and the antigen detection reagent strip clamping groove 9 are consistent in structure. The antibody detection reagent strip clamping groove 8 comprises an end part flange 8.1, a side flange 8.2 and a bottom support 8.3 positioned between the end part flange 8.1 and the side flange 8.2. The end part flanges 8.1 are U-shaped, and connecting strips 8.21 are arranged between the side flanges 8.2. The inner side of the upper plastic shell 1 is provided with a test paper compression column 13 and a test paper compression block 14, the test paper compression columns 13 are located at two ends of the antibody detection observation window 3, and the test paper compression block 14 is located between the antibody detection sampling hole 4 and the antibody detection observation window 3. The antibody detection reagent strip clamping groove 8 and the antigen detection reagent strip clamping groove 9 are respectively provided with an antibody detection reagent strip and an antigen detection reagent strip. The edges of the antibody detection observation window 3, the antibody detection sample adding hole 4, the buffer solution window 5, the antigen detection observation window 6 and the antigen detection sample adding hole 7 are all in an inverted horn mouth shape. The surface of the upper plastic shell 1 is provided with a two-dimensional code scanning area 12. And the antibody detection reagent strip clamping groove and the antigen detection reagent strip clamping groove are respectively internally provided with an antibody detection reagent strip and an antigen detection reagent strip, and the antibody detection reagent strip and the antigen detection reagent strip are respectively provided with a silver ion pad.
A B-shaped character is arranged beside the buffer liquid window 5; s-shaped samples are respectively arranged beside the antibody detection sample adding hole 4 and the antigen detection sample adding hole 7; the antibody detection observation window 3 is provided with IgM, IgG and C characters in sequence from one end close to the antibody detection sampling hole 4; the antigen detection observation window 6 is provided with the characters of Ag and C from one end close to the antigen detection sample adding hole 7. And a COVID-19 character is arranged between the observation window and the two-dimensional code scanning area 12.
The preparation steps of the antibody detection reagent strip are as follows:
(1) sample pad preparation: mixing and dissolving 4-8 g/L Tris, 4-7 g/L sodium caseinate, 6-15 g/L polyvinylpyrrolidone, 0.1-10g/L Tween 20, 0.1-10g/L Tetronic, 0.05-0.5 mg/ml blocking agent, 1/50 mouse anti-RBC and 0.02% Proclin300 in water, adjusting the pH value to 7.5-8.5, spraying the mixture on a glass fiber membrane, and drying;
(2) preparing a colloidal gold conjugate treatment pad: the whole process is carried out in a constant temperature heating system at 94-96 ℃, trisodium citrate aqueous solution is added into chloroauric acid aqueous solution to obtain colloidal gold, the pH of the obtained colloidal gold is adjusted to 6-10 by K2CO3 solution, the novel coronavirus antigen to be marked, BSA and PEG20000 are sequentially added, after stirring for 10-20min, the mixture is centrifuged for 10-20min at 2-8 ℃ and 9000 + 14000rpm, the precipitate is taken out, diluted to a certain concentration and sprayed on a polyester film, and the polyester film is dried for 20-24 h;
(3) preparing a silver ion treatment pad: dissolving silver nitrate in PBS at 0.1-1.0 mg/ml, adjusting pH to 7-8, spraying on glass fiber membrane, and baking at 30-60 deg.C for 20-24 hr;
(4) preparation of an analysis pad: diluting mouse anti-human IgM, mouse anti-human IgG and goat anti-mouse IgG antibody solutions, spraying the diluted solutions onto a nitrocellulose membrane at a concentration of 1.0-1.2 μ L/cm to serve as a detection line IgM, a detection line IgG and a quality control line C, and drying at 30-60 ℃;
(5) preparing a detection reagent strip: and connecting and assembling the sample pad, the silver ion treatment pad, the colloidal gold conjugate treatment pad, the analysis pad, the water absorption pad and the PVC base plate in a certain sequence, and cutting to obtain the COVID-19 IgG/IgM antibody detection reagent strip.
The process of detecting the antibody detection reagent strip needs the assistance of a buffer solution, and the preparation process of the buffer solution comprises the following steps: mixing 8-15g/L sodium carbonate, 1-10g/L sodium chloride, 1-10g/L ethylene diamine tetraacetic acid and 0.02% of liquid biological preservative in an aqueous solution, and adjusting the final pH =7.4 +/-0.1 of the mixed solution to obtain the buffer solution.
Mainly filters impurities in the sample and assists the sample to run on a plate for chromatography.
The preparation steps of the antigen detection reagent strip are as follows:
A. sample pad preparation: mixing and dissolving 4-8 g/L Tris, 4-7 g/L sodium caseinate, 6-15 g/L polyvinylpyrrolidone, 0.1-10g/L Tween 20, 0.1-10g/L Tetronic and 0.02% Proclin300 in water, adjusting the pH of the mixed solution to 7.5-8.5, spraying the mixed solution on a glass fiber membrane, and drying;
B. preparing a colloidal gold conjugate treatment pad: the whole process is carried out in a constant temperature heating system at 94-96 ℃, trisodium citrate aqueous solution is added into chloroauric acid aqueous solution to obtain colloidal gold, the pH of the obtained colloidal gold is adjusted to 6-10 by K2CO3 solution, novel coronavirus antibody, BSA and PEG20000 are sequentially added, after stirring for 10-20min, the mixture is centrifuged for 10-20min at 2-8 ℃ and 9000-;
C. preparing a silver ion treatment pad: dissolving silver nitrate in PBS at 0.1-1.0 mg/ml, adjusting pH to 7-8, spraying on glass fiber membrane, and baking at 30-60 deg.C for 20-24 hr;
D. preparation of an analysis pad: respectively spraying the solutions of the novel coronavirus antibody (detection line T) and the goat anti-mouse IgG antibody (quality control line C) on a nitrocellulose membrane, and drying at 30-60 ℃;
E. preparation of detection test paper: and connecting and assembling the sample pad, the colloidal gold conjugate treatment pad, the silver ion treatment pad, the analysis pad, the water absorption pad and the PVC base plate in a certain sequence, and cutting to obtain the COVID-19 antigen detection reagent strip.
The process of detecting the antigen detection reagent strip needs the assistance of lysate, and the preparation process of the lysate comprises the following steps: dissolving 1-10g/L sodium chloride, 0.5-5g/L Trizma-Base, 0.5-5g/L calf serum albumin, 1-10g/L LTriton X-504, 1-10g/L Tetronic and 0.02-0.04% proclin300 in water, and adjusting the final pH of the mixed solution to be 8.5 +/-0.1 to obtain the lysate.
The function of the lysate is mainly to crack the envelope protein of the new coronavirus and expose the nucleocapsid protein in the virus envelope, so that the new coronavirus can be detected. Detection method (as shown in fig. 5-6):
detection of novel coronavirus antibodies
A detection step: the sample (10. mu.L serum plasma or 20. mu.L whole blood) was added in the sample window "S", and 2 drops of buffer were added in the buffer window "B". After 15 min, the experimental results were observed.
And (4) judging a result: a mauve strip appears at any position of IgG in the second detection line or IgM in the third detection line, and the result is positive; IgG and IgM were negative with no magenta band. The C band was purple red regardless of whether the novel coronavirus antibody was detected.
Detection of novel coronavirus antigens
A detection step: inserting the sterile swab into nostril of patient, reaching the back of nasopharynx, wiping the surface of nasopharynx, taking out the swab, putting the swab into a lysis solution bottle, fully stirring, squeezing out the cotton swab and discarding. Add 3 drops of solution dropwise to the sample window "S". After 15 min, the experimental results were observed.
And (4) judging a result: if a purple red strip appears at the third detection line, the detection line is positive; if there is no purple-red band, it is negative. The C position was a purple red band regardless of whether the novel coronavirus antigen was detected.
Example 2
As shown in fig. 4, the difference from example 1 is that the antibody detection group does not include a buffer window; the upper cover comprises a joint 1 connected with the lower plate, a plate surface 2, an antibody detection observation window 3, an antibody sample adding hole 4, an antigen detection observation window 6 and an antigen sample adding hole 7; the upper cover and the lower plate are mutually embedded and fixed to form a combined diagnosis novel coronavirus antigen and antibody detection reagent plate; the antibody detection observation window 3 and the antigen detection observation window 6 are arranged in parallel, so that the result interpretation is not influenced, and the result misjudgment is avoided; the parallel arrangement of the antibody well 4 and the antigen well 7 can avoid cross contamination when a sample is added.
The antibody sample addition hole is different from the former antibody sample addition hole in that the sample addition hole and the buffer solution hole are the same hole, so the antibody detection sample addition mode can also be realized by adding a sample into the buffer solution sample addition hole 4 in advance and then dripping the buffer solution.
Claims (8)
1. A novel coronavirus antigen and antibody combined intelligent detection device comprises an upper plastic shell (1) and a lower plastic shell (2), it is characterized in that the upper plastic shell (1) is respectively provided with an antibody detection group and an antigen detection group, the antibody detection group comprises an antibody detection observation window (3) and an antibody detection sample adding hole (4), the antigen detection group comprises an antigen detection observation window (6) and an antigen detection sample adding hole (7), an antibody detection reagent strip clamping groove (8) and an antigen detection reagent strip clamping groove (9) are arranged on the inner side of the lower plastic shell (2), the antibody detection reagent strip clamping groove (8) corresponds to the antibody detection group, the antigen detection reagent strip clamping groove (9) corresponds to the antigen detection group, and an antibody detection reagent strip and an antigen detection reagent strip are respectively arranged in the antibody detection reagent strip clamping groove (8) and the antigen detection reagent strip clamping groove (9).
2. The novel combined intelligent detection device for the coronavirus antigen and the coronavirus antibody as claimed in claim 1, wherein a fixing bulge (11) is arranged on the inner side of the upper plastic shell (1), and the fixing bulge (11) surrounds the inner side edge of the upper plastic shell (1); the inner side of the lower plastic shell (2) is provided with a fixed boss groove (10), the fixed boss groove (10) surrounds the inner side edge of the lower plastic shell (2), and the fixed protrusion (11) is positioned in the fixed boss groove (10).
3. The novel coronavirus antigen-antibody combined intelligent detection device as claimed in claim 1, wherein the antibody detection reagent strip clamping groove (8) and the antigen detection reagent strip clamping groove (9) are consistent in structure.
4. The novel coronavirus antigen-antibody joint intelligent detection device according to claim 1, wherein the antibody detection reagent strip clamping groove (8) comprises an end rib (8.1), a side rib (8.2) and a bottom support (8.3) positioned between the end rib (8.1) and the side rib (8.2).
5. The novel coronavirus antigen and antibody joint intelligent detection device as claimed in claim 4, wherein the end flanges (8.1) are U-shaped, and a connecting strip (8.21) is arranged between the side flanges (8.2).
6. The novel coronavirus antigen and antibody joint intelligent detection device as claimed in claim 1, wherein a test paper compression column (13) and a test paper compression block (14) are arranged on the inner side of the upper plastic shell (1), the test paper compression columns (13) are positioned at two ends of the antibody detection observation window (3), and the test paper compression block (14) is positioned between the antibody detection sample adding hole (4) and the antibody detection observation window (3).
7. The novel coronavirus antigen-antibody combined intelligent detection device as claimed in claim 1, wherein the edges of the antibody detection observation window (3), the antibody detection sample adding hole (4), the antigen detection observation window (6) and the antigen detection sample adding hole (7) are all in an inverted bell mouth shape, and a two-dimensional code scanning area (12) is arranged on the surface of the upper plastic shell (1).
8. The novel coronavirus antigen-antibody combined intelligent detection device as claimed in claim 1, wherein the antibody detection group further comprises a buffer window (5).
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