CN1595121A - Optical waveguide biochemical sensor and measurement system based on free space coupling - Google Patents
Optical waveguide biochemical sensor and measurement system based on free space coupling Download PDFInfo
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- CN1595121A CN1595121A CN 200410025387 CN200410025387A CN1595121A CN 1595121 A CN1595121 A CN 1595121A CN 200410025387 CN200410025387 CN 200410025387 CN 200410025387 A CN200410025387 A CN 200410025387A CN 1595121 A CN1595121 A CN 1595121A
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Abstract
It is a light wave-guide biochemistry sensor and its measurement system based on free space couple, which belongs to accuracy analysis and measurement device. The light wave guide biochemistry sensor is fixed on the center of the upper disc of the optics rotation desk, which comprises upper metal film, upper optics glass sheet, solvent chamber, ground optics glass sheet, ground metal film, cushion block, platform and micrometer screw gage. The upper metal film deposits on the upper optics glass sheet and the ground metal film deposits on the ground optics glass sheet. The metal film surface of upper optics glass sheet faces upward and that of the ground optics glass sheet faces downward. The upper optics glass sheet, solvent chamber, ground glass sheet are orderly folded and laid on the platform with cushion block supported, which are tightly supported through micrometer screw gage.
Description
Technical field
What the present invention relates to is that a kind of sensor reaches by its measuring system of forming, particularly a kind of optical waveguide biochemical sensor and measuring system based on the free space coupling of not having the prism-coupled unit.Be used for rigorous analysis and surveying instrument field.
Background technology
Since the late nineteen eighties in last century, be subjected to paying attention to widely based on the sensor of surface plasma resonance (SPR), optical waveguide and optical fiber technology.This class sensor construction is simple, easy to use, reliable, can be applicable to the measurement of a plurality of parameters in field such as biology, chemistry and physics, especially in fields such as protein structure, antibody antigen detections important use is arranged.A common feature of this class sensor is the evanescent field region that testing sample is in resonant mode (surface plasma-wave and optical guided wave).Therefore, this class sensor is also referred to as the evanescent field sensor.Shortcoming: (1) is for the sensor based on leakage optical waveguide and optical fiber technology, the width of resonant mode ATR absorption peak may be very narrow, but its sample is usually as the overlayer of optical waveguide or optical fiber, and the luminous energy in the overlayer only accounts for a few percent of the whole luminous energy of resonant mode.Therefore, can not do very highly usually based on the sensitivity of the evanescent field sensor of revealing optical waveguide and optical fiber technology.(2) for SPR type sensor,, have higher detection efficiency,, be difficult to the detection sensitivity that reaches very high equally because the absorption of metal film causes the width of its ATR absorption peak very big though there is luminous energy to be in detecting area greater than half.
Find by retrieval, Chinese patent publication number: 1396445A, name is called: dual-surface metal waveguide measuring method and device thereof have proposed double-sided metal and have coated waveguide sensor in this patent of invention.This dual-surface metal waveguide device from top to bottom is made of coupled apparatus, upper strata metal film, thin layer to be measured, lower metal film, and thin layer to be measured and upper/lower layer metallic film constitute the double-level-metal waveguiding structure, and laser is mainly propagated in thin layer to be measured.An important feature of this sensor is to utilize coupled apparatus such as prism that laser is coupled into the sensor detection cavity from the outside, utilizes the advantageous property of metal carbonyl coat to realize surveying.But, using coupled apparatuses such as prism in this sensor, this makes that method is very limited on require the less fields of measurement of device size, is realizing aspect the low-angle detection certain difficulty being arranged simultaneously.
Summary of the invention
The present invention is directed to the defective that exists in the above technology, proposed a kind of optical waveguide biochemical sensor and measuring system, make it based on the free space coupling principle based on the free space coupling, light directly is coupled to the ducting layer from metal film and goes, need not to use coupled apparatus, can make the detection angle of incident light very little, utilize the good characteristic of waveguide high order guided modes, simple in structure, highly sensitive, cost is low, is easy to processing and manufacturing, and require low, practical to the ranges of indices of refraction of testing sample.
The present invention is achieved by the following technical solutions, and optical waveguide biochemical sensor of the present invention comprises: upper strata metal film, upper strata optical glass sheet, solution cavity, lower floor's optical glass sheet, lower metal film, cushion block, platform, screw-thread micrometer.The upper strata metal film deposition is on the optical glass sheet of upper strata, and lower metal film is deposited on lower floor's optical glass sheet.The metal film of upper strata optical glass sheet is towards last, and the metal film of lower floor's optical glass sheet is downward, and upper strata optical glass sheet, solution cavity, lower floor's glass sheet stack successively, and are placed on the platform, prop up with a cushion block, by the turn screw-thread micrometer total are pushed against.Upper strata optical glass sheet, lower floor's optical glass sheet are clamped by upper strata metal film, lower metal film respectively, make upper strata metal film, upper strata optical glass sheet, solution cavity and lower floor's optical glass sheet, lower metal film constitute an optical waveguide, it is the ducting layer of optical glass sheet, solution cavity and lower floor's optical glass sheet formation optical waveguide at the middle and upper levels.Being drilled with two apertures on lower floor's optical glass sheet, is respectively the solution inlet and the taphole of sensor, and testing sample enters solution cavity by the solution inlet, and waste liquid is discharged by taphole.
The measuring system that the present invention is made up of the optical waveguide biochemical sensor comprises: optical waveguide biochemical sensor, optics rotation platform, sample delivery part and photoelectricity receiving unit.The optical waveguide biochemical sensor is fixed on the optics rotation platform, and the center that guarantees the center of its upper strata metal film and rotation platform top rotary table is at same position, and the optics rotation platform supports overall optical waveguide biochemical sensor.
The optics rotation platform is made of base for supporting, driving interface and two-layer up and down rotating disk, base for supporting is supporting two-layer rotating disk up and down, driving interface is by the side of screw retention at base for supporting, the rotation center of two-layer rotating disk is coaxial up and down, when computing machine makes the top rotary table uniform rotation by driving interface, lower rotary table relatively and top rotary table rotate in the opposite direction with twice angular velocity.
Sample delivery partly comprises: sample cell, rubber tube, constant flow pump, waste collection container.Rubber tube has three sections, and the inlet of constant flow pump and sample cell couple together by first section rubber tube.The constant current delivery side of pump is connected by second section rubber tube with the solution inlet.The taphole of optical waveguide biochemical sensor solution cavity is connected by the 3rd section rubber tube with the waste collection container.Testing sample is drawn from sample cell by constant flow pump, enters solution cavity by rubber tube from the solution of optical waveguide biochemical sensor inlet, and waste liquid flows in the waste collection container by rubber tube from the taphole of sensor solution cavity simultaneously.
The photoelectricity receiving unit comprises: laser instrument, polarizer, optics aperture, photodetector and computing machine.Photodetector is fixed on the outer ring of lower rotary table of optics rotation platform, and laser instrument, polarizer, optics aperture are installed in outside the optics rotation platform, and they keep contour coaxial, and optical axis points to the center of optical waveguide biochemical sensor.The laser instrument emitted laser is by polarizer outgoing single polarization, behind the optics aperture, incide the central shaft of optical waveguide biochemical sensor, photodetector, laser instrument is about the central shaft symmetry of optical waveguide biochemical sensor, photodetector is fixed on the lower rotary table outer ring of optics rotation platform, the optics rotation platform is under the situation that laser positions is fixed, at the uniform velocity change incident light relatively and the incident angle of sensor surface, photodetector is with the reverse rotation of twice angular velocity simultaneously, reflected light is dropped on the photodetector, photodetector links to each other with computing machine, computer acquisition, the signal that conversion and storage photodetector receive.
When rotation platform is static, constant flow pump is transported to testing sample in the solution cavity of optical waveguide biochemical sensor by rubber tube, open laser instrument, shoot laser passes through polarizer, select single polarization, center through the upper strata metal film of the light incident light waveguide biochemical sensor that obtains certain spot size behind the optics aperture, the reflected light at air and metal film interphase place, upper strata enters photodetector, photodetector links to each other with the digital to analog converter (A/D) of computing machine, computing machine begins data acquisition, drive the optics rotation platform by programmed control, the incident angle of the relative optical waveguide biochemical sensor of laser just changes, observe the result of image data, select to stop to drive the optics rotation platform after the suitable incident angle, with this incident angle as working point Continuous Observation record, by the change of detection of reflected light intensity, can the inverting solution cavity in the variations in refractive index of testing sample.
Fiber waveguide device of the present invention has the following advantages: based on the free space coupling, need not prism as coupling unit, can realize small angle measurement like this; Clad is a metal, can be unrestricted as the sample refractive index of guided wave core layer, and promptly can be liquid, also can be gas; The thickness of core layer can reach the submillimeter magnitude, has not only enlarged the parking space of sample, and for the superelevation rank guided mode that forms optical waveguide provides possibility, increases glassy layer simultaneously as ducting layer, and thermonoise is produced certain inhibiting effect; Though clad adopts metallic film can cause absorption, theory points out, the absorption meeting increases along with the thickness of core layer and reduces, and the width of ATR absorption peak reduces 1-2 magnitude than spr sensor among the present invention; The propagation constant of superelevation rank guided mode is very sensitive to the variation of the refractive index (sample concentration and absorption) of sample; Sample is in the great oscillating field of luminous energy density, and detection efficiency is high; The existing evanescent field sensor of the remolding sensitivity of optical waveguide resonant mode is high one more than the order of magnitude.Also owing to there is not prism coupler, make that device itself can be littler, solution cavity can be accomplished a millimeter magnitude simultaneously, and this is to excite highly sensitive high order guided modes to create condition.The present invention has the structural design of innovation, and good in anti-interference performance is simple in structure, and cost is low, is easy to processing, and low, practical to the ranges of indices of refraction requirement of sample, has brilliant market developing prospect.
Description of drawings
Fig. 1 measuring system synoptic diagram of the present invention
Fig. 2 optical waveguide biochemical sensor of the present invention structural representation
Fig. 3 optical waveguide biochemical sensor of the present invention vertical view
Fig. 4 optical waveguide biochemical sensor of the present invention front view
Fig. 5 optical waveguide biochemical sensor of the present invention and optics rotation platform wiring layout
Embodiment
As Fig. 1, Fig. 2, Fig. 3, Fig. 4, shown in Figure 5, optical waveguide biochemical sensor 1 of the present invention comprises: upper strata metal film 9, upper strata optical glass sheet 10, solution cavity 11, lower floor's optical glass sheet 12, lower metal film 13, cushion block 14, platform 15, screw-thread micrometer 16.Upper strata metal film 9 is deposited on the upper strata optical glass sheet 10, and lower metal film 13 is deposited on lower floor's optical glass sheet 12.With the metal film of upper strata optical glass sheet 10 towards last, the metal film of lower floor's optical glass sheet 12 is downward, and upper strata optical glass sheet 10, solution cavity 11, lower floor's glass sheet 12 stack successively, and is placed on the platform 15, prop up with a cushion block 14, total is pushed against by turn screw-thread micrometer 16.Optical glass sheet 10, lower floor's optical glass sheet 12 are clamped by upper strata metal film 9, lower metal film 13 respectively at the middle and upper levels for they, make upper strata metal film 9, upper strata optical glass sheet 10, solution cavity 11 and lower floor's optical glass sheet 12, lower metal film 13 constitute an optical waveguide, it is the ducting layer of optical glass sheet 10, solution cavity 11 and lower floor's optical glass sheet 12 formation optical waveguides at the middle and upper levels.
Being drilled with two apertures on lower floor's optical glass sheet 12, is respectively the solution inlet 17 and the taphole 18 of sensor.Testing sample enters solution cavity 11 by solution inlet 17, and waste liquid is discharged by taphole 18.
The present invention by above-mentioned optical waveguide biochemical sensor form measuring system comprise: optical waveguide biochemical sensor 1, optics rotation platform 2, sample delivery part 3 and photoelectricity receiving unit 4, optics rotation platform 2 is by two-layer rotating disk 5,6 up and down, base for supporting 7 and driving interface 8 constitute, base for supporting 7 is supporting two-layer rotating disk 5,6 up and down, driving interface 8 is by the side of screw retention at base for supporting 7, and the rotation center of two-layer up and down rotating disk 5,6 is coaxial; Sample delivery part 3 comprises: sample cell 19, rubber tube 20, constant flow pump 21, waste collection container 22 are connected in turn sample cell 19, constant flow pump 21, solution inlet 17, taphole 18, waste collection container 22 by rubber tube 20; Photoelectricity receiving unit 4 comprises: laser instrument 23, polarizer 24, optics aperture 25, photodetector 26 and computing machine 27, laser instrument 23, polarizer 24, optics aperture 25 is fixed on outside the optics rotation platform 2, they keep contour coaxial, optical axis points to the center of upper strata metal film 9, photodetector 26, laser instrument 23 is about the central shaft symmetry of optical waveguide biochemical sensor 1, photodetector 26 is fixed on the optics rotation platform 2, photodetector 26 links to each other with computing machine 27, optical waveguide biochemical sensor 1 is fixed on the optics rotation platform 2, the center of the upper strata metal film 9 of optical waveguide biochemical sensor 1 and the center of rotation platform top rotary table 5 are at same position, optics rotation platform 2 supports optical waveguide biochemical sensor 1, and optical waveguide biochemical sensor 1 is fixed on the center of the top rotary table 5 of optics rotation platform 2.
When motor drove top rotary table 5 uniform rotation by driving interface 8, lower rotary table 6 rotated with twice angular velocity in the opposite direction with respect to top rotary table 5, and photodetector 26 is fixed on the outer ring of lower rotary table 6.
Optics rotation platform 2 under the situation of laser instrument 23 stationkeeping, at the uniform velocity change incident light relatively with the incident angle of optical waveguide biochemical sensor 1, while photodetector 26 drops on the photodetector 26 reflected light with the reverse rotation of twice angular velocity.
Claims (5)
1, a kind of optical waveguide biochemical sensor based on the free space coupling, comprise: upper strata metal film (9), upper strata optical glass sheet (10), solution cavity (11), lower floor's optical glass sheet (12), lower metal film (13), cushion block (14), platform (15), screw-thread micrometer (16), it is characterized in that, upper strata metal film (9) is deposited on the upper strata optical glass sheet (10), lower metal film (13) is deposited on lower floor's optical glass sheet (12), the metal film of upper strata optical glass sheet (10) is towards last, the metal film of lower floor's optical glass sheet (12) is downward, upper strata optical glass sheet (10), solution cavity (11), lower floor's glass sheet (12) stacks successively, and be placed on the platform (15), prop up with a cushion block (14), by turn screw-thread micrometer (16) total is pushed against, it is optical glass sheet (10) at the middle and upper levels, lower floor's optical glass sheet (12) is respectively by upper strata metal film (9), lower metal film (13) is clamped, upper strata metal film (9), upper strata optical glass sheet (10), solution cavity (11) and lower floor's optical glass sheet (12), lower metal film (13) constitutes an optical waveguide, and it is optical glass sheet (10) at the middle and upper levels, solution cavity (11) and lower floor's optical glass sheet (12) constitute the ducting layer of optical waveguide.
2, the optical waveguide biochemical sensor based on the free space coupling according to claim 1 is characterized in that, is drilled with two apertures on lower floor's optical glass sheet (12), is respectively solution inlet (17) and the taphole (18) of sensor.
3, a kind of measuring system of forming based on the optical waveguide biochemical sensor of free space coupling, comprise: optical waveguide biochemical sensor (1), optics rotation platform (2), sample delivery part (3) and photoelectricity receiving unit (4) four parts, it is characterized in that, optics rotation platform (2) is by two-layer rotating disk (5) up and down, (6), base for supporting (7) and driving interface (8) constitute, base for supporting (7) is supporting two-layer rotating disk (5) up and down, (6), driving interface (8) is by the side of screw retention in base for supporting (7), two-layer up and down rotating disk (5), (6) rotation center is coaxial; Sample delivery part (3) comprising: sample cell (19), rubber tube (20), constant flow pump (21), waste collection container (22) are connected in turn sample cell (19), constant flow pump (21), solution inlet (17), taphole (18), waste collection container (22) by rubber tube (20); Photoelectricity receiving unit (4) comprising: laser instrument (23), polarizer (24), optics aperture (25), photodetector (26) and computing machine (27), laser instrument (23), polarizer (24), optics aperture (25) is fixed on outside the optics rotation platform (2), they keep contour coaxial, optical axis points to the center of upper strata metal film (9), photodetector (26), laser instrument (23) is about the central shaft symmetry of optical waveguide biochemical sensor (1), photodetector (26) is fixed on the optics rotation platform (2), photodetector (26) links to each other with computing machine (27), optical waveguide biochemical sensor (1) is fixed on the optics rotation platform (2), the center of the upper strata metal film (9) of optical waveguide biochemical sensor (1) and the center of rotation platform top rotary table (5) are at same position, optics rotation platform (2) supports optical waveguide biochemical sensor (1), and optical waveguide biochemical sensor (1) is fixed on the center of the top rotary table (5) of optics rotation platform (2).
4, the measuring system of forming based on the optical waveguide biochemical sensor of free space coupling according to claim 3, it is characterized in that, when motor drives top rotary table (5) uniform rotation by driving interface (8), lower rotary table (6) rotates with twice angular velocity in the opposite direction with respect to top rotary table (5), and photodetector (26) is fixed on the outer ring of lower rotary table (6).
5, the measuring system of forming based on the optical waveguide biochemical sensor of free space coupling according to claim 3, it is characterized in that, optics rotation platform (2) is under the situation of laser instrument (23) stationkeeping, at the uniform velocity change incident light relatively and the incident angle of optical waveguide biochemical sensor (1), photodetector (26) drops on the photodetector (26) reflected light with the reverse rotation of twice angular velocity simultaneously.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 200410025387 CN1292245C (en) | 2004-06-24 | 2004-06-24 | Optical waveguide biochemical sensor and measurement system based on free space coupling |
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| CN 200410025387 CN1292245C (en) | 2004-06-24 | 2004-06-24 | Optical waveguide biochemical sensor and measurement system based on free space coupling |
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| CN1595121A true CN1595121A (en) | 2005-03-16 |
| CN1292245C CN1292245C (en) | 2006-12-27 |
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| CN 200410025387 Expired - Fee Related CN1292245C (en) | 2004-06-24 | 2004-06-24 | Optical waveguide biochemical sensor and measurement system based on free space coupling |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100401041C (en) * | 2005-06-09 | 2008-07-09 | 上海交通大学 | Light waveguide absorption type gas sensor and measuring system |
| CN102692392A (en) * | 2011-03-25 | 2012-09-26 | 上海光刻电子科技有限公司 | Device for measuring gas and liquid refractive indexes |
| CN102967593A (en) * | 2012-11-28 | 2013-03-13 | 南通大学 | Method of optical waveguide enhancement mechanism and Raman spectrometer |
| CN103278455A (en) * | 2013-05-16 | 2013-09-04 | 宁波大学 | Measurement device and measurement method of optical parameters of dielectric film |
| CN105044042A (en) * | 2015-05-08 | 2015-11-11 | 铜仁学院 | Concentration detection method of manganese in trace amount in drinking water and waveguide used therein |
| CN105548093A (en) * | 2016-01-23 | 2016-05-04 | 宜春学院 | High-sensitivity oscillating field optical waveguide sensor |
| CN105891152A (en) * | 2014-10-01 | 2016-08-24 | 上海光刻电子科技有限公司 | Refractive index measurement method with wide range |
-
2004
- 2004-06-24 CN CN 200410025387 patent/CN1292245C/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100401041C (en) * | 2005-06-09 | 2008-07-09 | 上海交通大学 | Light waveguide absorption type gas sensor and measuring system |
| CN102692392A (en) * | 2011-03-25 | 2012-09-26 | 上海光刻电子科技有限公司 | Device for measuring gas and liquid refractive indexes |
| CN102967593A (en) * | 2012-11-28 | 2013-03-13 | 南通大学 | Method of optical waveguide enhancement mechanism and Raman spectrometer |
| CN103278455A (en) * | 2013-05-16 | 2013-09-04 | 宁波大学 | Measurement device and measurement method of optical parameters of dielectric film |
| CN103278455B (en) * | 2013-05-16 | 2015-10-28 | 宁波大学 | A kind of measurement mechanism of dielectric film optical parametric and measuring method |
| CN105891152A (en) * | 2014-10-01 | 2016-08-24 | 上海光刻电子科技有限公司 | Refractive index measurement method with wide range |
| CN105044042A (en) * | 2015-05-08 | 2015-11-11 | 铜仁学院 | Concentration detection method of manganese in trace amount in drinking water and waveguide used therein |
| CN105548093A (en) * | 2016-01-23 | 2016-05-04 | 宜春学院 | High-sensitivity oscillating field optical waveguide sensor |
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| CN1292245C (en) | 2006-12-27 |
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