CN1749735A - Plastic made non-grating type surface plasma excimer resonant optic coupler - Google Patents
Plastic made non-grating type surface plasma excimer resonant optic coupler Download PDFInfo
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- CN1749735A CN1749735A CN 200510019519 CN200510019519A CN1749735A CN 1749735 A CN1749735 A CN 1749735A CN 200510019519 CN200510019519 CN 200510019519 CN 200510019519 A CN200510019519 A CN 200510019519A CN 1749735 A CN1749735 A CN 1749735A
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Abstract
The present invention makes non-grating surface plasmon resonance (SPR) optical coupler with plastic to replace optical glass, and belongs to the field of waveguide optics and biological sensing technology. The disposable low cost optical coupler made with low refractive index plastic has low cost, less noise, raised SPR biosensor S/N ratio, convenient instrument operation, and convenient integration with other parts. The SPR biosensor of the present invention may be used in clinical detection, sanitary environment monitoring, industrial production and basic life science research relative to mutual action of macro biomolecules.
Description
Technical field
The invention belongs to waveguide optical and biosensor technique crossing domain, specifically is to adopt non-glass materials---the integrated resonance of non-grating type surface plasma excimer (Surface PlasmonResonance, SPR) optical coupler that plastics are made.
Background technology
The living organism that lives is according to certain time sequence, in the result of specific steric interaction by biomacromolecules such as protein, nucleic acid.Because the geometric scale of biomacromolecule is a nanometer scale, in basic life science and clinical treatment detection, for the quantitative test interaction of biomacromolecules, usually the object that need be studied with specific material marks such as radioelement, fluorescent material or enzymes, thus make the interaction of the biomacromolecule of antigen-antibody response, DNA hybridization and so on produce the physical phenomenon of human eye or the energy perception of instrument institute.(Surface Plasmon Resonance SPR), also has the people that it is translated into the resonance of surface plasma mould to surface plasmon resonance.SPR is the interactional a kind of optical phenomena of visible light and metal free electron gas (Otto A.1968, Kretchmann E.1971).Because the SPR absorption peak is to dielectric change of refractive sensitivity of metal surface absorption, therefore need not the mark testing sample just can utilize surface plasmon resonance biosensor monitoring bio macromolecular reaction in real time.This biological monitoring technology highly sensitive, easy to operate and free from environmental pollution has obtained using more and more widely (Nylander, C.Etal, 1983) from the eighties since latter stage.
Surface plasmons is the electromagnetic wave of metal-dielectric system natural mode.This electromagnetic wave can be propagated, decay perpendicular to the interface direction index along metal-dielectric interface.Under given conditions, visible light is coupled by prism or grating can excite the surface plasmons of given electromagnetism system, produces the surface plasmon resonance phenomenon.The surface plasmon resonance biosensor of prism-coupled type adopts the optics unitized construction of Kretchmann invention usually at present, this instrument develops comparative maturity, can find the company of four tame production and selling surface plasmon resonance biosensors such as Biacore, Biotul, Texas Instruments and Quantech on Internet.The surface plasmon resonance biosensor of prism-coupled type can be divided into three kinds of angle scanning type, length scanning type and luminosity classes again.Have only the surface plasmon resonance biosensor (Fig. 1) of angle scanning type that the ripe spectral method (Xiao Caide, 1999) of separating is arranged at present.
In existing surface plasmon resonance biosensor, usually will be to detected biological sample such as aids antibody, responsive biological sample such as AIDS virus expressed proteins are coated on the surface of metal film in advance, and the carrier of metal film is a cover glass.Cover glass, metal film and biological membrane have constituted the sensitive membrane of surface plasmon resonance biosensor together, use once.The glass of sensitive membrane is attached on the prism of high index of refraction by optical match medium cedar oil; Biological sample one side of sensitive membrane contacts with the damping fluid in the sample cell.The monochromatic light of plane of polarization in the plane of incidence arrives the interface of metal film and biological sample from prism transmission under total reflection condition, excite the surface plasmons at this interface, causes producing resonance absorbing peak on the relation curve of intensity of reflected light and incident angle.If be added in the sample cell antibody be coated on metal film on the combining of antigen, the thick moisture film of the about 4nm of one deck beyond the antigenic membrane of metallic film surface is replaced by the antibody protein film.Under the compact arranged situation of antibody molecule, the refractive index of this layer film will be increased to 1.5 from 1.33.The dielectric variation of metallic film surface causes the SPR absorption spectra to change, by real-time monitoring SPR absorption spectra just change can the quantitative measurement biomacromolecule molecular structure, binding constant and binding capacity.
The reaction of biomacromolecule is carried out at water usually.The interface that has glass prism and coupling medium oil interface, coupling medium oil and cover glass interface, cover glass and metal film interface, metal film and biological membrane interface, biological membrane and water in the existing surface plasmon resonance biosensor light path.Can interfere with each other the generation undesired signal from the light of these boundary reflections.Usually adopt glass to improve to make prism refractive index, increase and excite the methods such as light wavelength of plasmon to be used to suppress this undesired signal, but these methods can not tackle the problem at its root, and also need to utilize hardware and software to extract the signal of biomacromolecule reaction from the SPR spectrum.
Summary of the invention
The optics coupling device of existing surface plasmon resonance biosensor is made up of glass prism, coupling medium oil, cover glass, metal film, biological membrane etc.The objective of the invention is to adopt plastic material make a kind of can be with above-mentioned sectoral integration, disposable surface plasma excimer resonant optic coupler, and can further facilitate integrated with other parts of surface plasmon resonance checkout equipment.
A kind of non-grating type surface plasma excimer resonant optic coupler of the present invention, the employing plastic material is made, plane upper section in incident ray and reflection ray formation is shaped as polygon or semicircle, be random length perpendicular to cross-wise direction, its geometric configuration is polyhedron, semisphere, cut the combination of the semisphere or the above-mentioned shape of spherical crown.
Non-grating type surface plasma excimer resonant optic coupler of the present invention, on the optical coupler of any geometric configuration of the above, sample cell with the holding liquid of the integrated manufacturing of optical coupler is arranged, and that sample cell has is one or more, shape and volume are not limit.
Non-grating type surface plasma excimer resonant optic coupler of the present invention is characterized in that at least one surface plated metal film, perhaps on part or all of metal film bag by biological sample.
Non-grating type surface plasma mould resonance optical coupler of the present invention is applicable to following plasma mould resonance instrument: angle scanning class surface plasma mould resonance instrument, length scanning class surface plasma mould resonance instrument, photometric measurement class surface plasma mould resonance instrument and micro-imaging class surface plasma mould resonance instrument.
The present invention adopts plastics to replace optical glass material to make the non-grating type surface plasma excimer resonant optic coupler.Plastic manufacturing process can be made into one sample cell and total reflection prism, directly at the prism surface metal-coated membrane, and on metal film, wrap by biological sample, traditional prism, metal film and sensitive membrane is integrated, and the present invention the cost of whole surface plasma excimer resonant optic coupler is dropped to so low so that the use once of SPR optical coupler is become a reality.It has eliminated and has had glass prism and coupling medium oil interface, coupling medium oil and cover glass interface, cover glass and a plurality of interfaces such as metal film interface, metal film and biological membrane interface in the existing surface plasmon resonance biosensor light path, thereby has eliminated the undesired signal that these boundary reflection light interfere with each other generation.The surface plasmon resonance biosensor of invention no longer needs cover glass, therefore no longer needs cedar oil to make the optical match medium, thereby the problem that no longer includes the oil contamination instrument exists; Optical coupler and fluid sample pond manufacture one, and sample cell no longer includes the problem of leaking, and easy greatly practical operation, accelerate detection speed, help this The Application of Technology and promote.
Description of drawings
Fig. 1 angle scanning surface plasma mould resonance detection device synoptic diagram
The angle scanning surface plasma mould of Fig. 2 plastics manufacturing integrated optical coupler that resonates
The integrated optical coupler vertical view of 14 sample cells that Fig. 3 plastics are made
Sign expression among the figure: 1 laser generator, 2 optical prisms, 3 optical match media, 4 cover glasses, 5 gold medal films, 6 sensitive membrane, 7 detect solution example, 8 sample cells, 9 optical signal detectors, 10 rotational angle devices, 11 controls, record, calculating and display station, 12 optical coupling device locating slots, 13 optical coupling device pilot holes.
Embodiment
Fig. 2 represents the integrated optical coupler of a kind of plastics manufacturing angle scanning surface plasmon resonance of the present invention.Its main part 2 employing refractive indexes are 1.58 polystyrene manufacturing, be an integrally manufactured sample cell 8 above, the golden film of 50 nanometer thickness has been plated in the sample cell bottom, according to detecting the needs corresponding biochemical sensitive membrane that has been covered, become prism, golden film, the incorporate surface plasmons optics of sensitive membrane coupling device above the gold film.It is connected location with pilot hole 13 with the rotating operation platform by locating slot 12, and convenient moving or replacing realizes in time detecting fast.
Fig. 3 represents a kind of surface plasma excimer resonant optic coupler that 5 sample cells are arranged of the present invention.These a plurality of sample cells can be as required, and bag is reached simultaneously a certain sample is finished the multiple different purpose that detects by different sensitive membrane; Also can wrap, reach different articles for use are done purpose identical or that relatively detect by identical sensitive membrane.Obviously, the number that increases sample cell can further reduce the real cost of optical coupler of the present invention, helps the realization of biochemical investigation real-time and the application of SPR detection technique.
Claims (7)
1, a kind of non-grating type surface plasma excimer resonant optic coupler is characterized in that: this surface plasma excimer resonant optic coupler is to adopt plastics to make.
2, non-grating type surface plasma excimer resonant optic coupler as claimed in claim 1 is characterized in that the plane upper section that its incident ray and reflection ray constitute is shaped as polygon or semicircle, is being random length perpendicular to cross-wise direction.
3,, it is characterized in that it has at least a surface to plate metal film as claim 1 or 2 described non-grating type surface plasma excimer resonant optic couplers.
4, non-grating type surface plasma excimer resonant optic coupler as claimed in claim 3 is characterized in that bag is by biological sample on the part or all of metal film of metal film of surface plating.
5, non-grating type surface plasma excimer resonant optic coupler as claimed in claim 1 is characterized in that its geometric configuration is polyhedron, semisphere, cuts the combination of the semisphere or the above-mentioned shape of spherical crown.
6, non-grating type surface plasma excimer resonant optic coupler as claimed in claim 5, it is characterized in that on the optical coupler of described any geometric configuration, sample cell with the Sheng tracer liquid of the integrated manufacturing of optical coupler is arranged, and that sample cell has is one or more, shape and volume are not limit.
7, the application of the described non-grating type surface plasma excimer resonant optic coupler of claim 1 is characterized in that being used for angle scanning class surface plasmon resonance instrument, length scanning class surface plasmon resonance instrument, photometric measurement class surface plasmon resonance instrument, micro-imaging class surface plasmon resonance instrument.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510019519 CN1749735A (en) | 2005-09-29 | 2005-09-29 | Plastic made non-grating type surface plasma excimer resonant optic coupler |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510019519 CN1749735A (en) | 2005-09-29 | 2005-09-29 | Plastic made non-grating type surface plasma excimer resonant optic coupler |
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| CN1749735A true CN1749735A (en) | 2006-03-22 |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102590147A (en) * | 2012-02-01 | 2012-07-18 | 中国科学院化学研究所 | Surface plasma resonance system capable of adjusting sensitivity and dynamic range and detection method thereof |
| TWI384214B (en) * | 2008-01-18 | 2013-02-01 | Nat Univ Chung Cheng | Biological sensing device and its system |
| CN103245635A (en) * | 2012-02-07 | 2013-08-14 | 中国科学院微电子研究所 | Sensor based on guided wave resonance and preparation method thereof |
| US8730468B2 (en) | 2008-02-01 | 2014-05-20 | Rare Light, Inc. | Methods, devices and kits for peri-critical reflectance spectroscopy |
| US8970838B2 (en) | 2011-04-29 | 2015-03-03 | Avolonte Health LLC | Method and apparatus for evaluating a sample through variable angle Raman spectroscopy |
| US9041923B2 (en) | 2009-04-07 | 2015-05-26 | Rare Light, Inc. | Peri-critical reflection spectroscopy devices, systems, and methods |
| CN102317758B (en) * | 2009-02-18 | 2016-06-01 | 皇家飞利浦电子股份有限公司 | Sensing device for detecting objects matter |
| CN106855511A (en) * | 2016-12-07 | 2017-06-16 | 大连理工大学 | High sensitivity infrared band surface plasmon resonance biosensor |
-
2005
- 2005-09-29 CN CN 200510019519 patent/CN1749735A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI384214B (en) * | 2008-01-18 | 2013-02-01 | Nat Univ Chung Cheng | Biological sensing device and its system |
| US8730468B2 (en) | 2008-02-01 | 2014-05-20 | Rare Light, Inc. | Methods, devices and kits for peri-critical reflectance spectroscopy |
| CN102317758B (en) * | 2009-02-18 | 2016-06-01 | 皇家飞利浦电子股份有限公司 | Sensing device for detecting objects matter |
| US9041923B2 (en) | 2009-04-07 | 2015-05-26 | Rare Light, Inc. | Peri-critical reflection spectroscopy devices, systems, and methods |
| US8970838B2 (en) | 2011-04-29 | 2015-03-03 | Avolonte Health LLC | Method and apparatus for evaluating a sample through variable angle Raman spectroscopy |
| CN102590147A (en) * | 2012-02-01 | 2012-07-18 | 中国科学院化学研究所 | Surface plasma resonance system capable of adjusting sensitivity and dynamic range and detection method thereof |
| CN102590147B (en) * | 2012-02-01 | 2014-01-29 | 中国科学院化学研究所 | Surface plasma resonance system capable of adjusting sensitivity and dynamic range and method thereof |
| CN103245635A (en) * | 2012-02-07 | 2013-08-14 | 中国科学院微电子研究所 | Sensor based on guided wave resonance and preparation method thereof |
| CN106855511A (en) * | 2016-12-07 | 2017-06-16 | 大连理工大学 | High sensitivity infrared band surface plasmon resonance biosensor |
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