CN201434868Y - Waveguide-coupled metal photonic crystal biosensor - Google Patents
Waveguide-coupled metal photonic crystal biosensor Download PDFInfo
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- CN201434868Y CN201434868Y CN 200920106042 CN200920106042U CN201434868Y CN 201434868 Y CN201434868 Y CN 201434868Y CN 200920106042 CN200920106042 CN 200920106042 CN 200920106042 U CN200920106042 U CN 200920106042U CN 201434868 Y CN201434868 Y CN 201434868Y
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Abstract
The utility model provides a waveguide-coupled metal photonic crystal biosensor used for highly sensitive sensing of concentration of biomolecules and specific response of bioactive molecules. The biosensor comprises a substrate, a waveguide layer and a metal photonic crystal prepared on the waveguide layer. When in detection, light emitted from a light source irradiates on the metal photonic crystal at a certain angle, an optical detector detects the extinction spectra of transmission light passing through the waveguide layer and the substrate or the extinction spectra of the reflected lightpassing through the metal photonic crystal, a receptor is fixed on the metal photonic crystal, sample solution passes through the surface of the metal photonic crystal, and then the detector detects the extinction spectra of transmission light passing through the waveguide layer and the substrate or the extinction spectra of the reflected light passing through the metal photonic crystal, conductssecondary extinction spectra calculation on the two times of extinction spectra, therefore, the quantitative detection for ligand concentration is realized. The biosensor has the advantages of high sensitiveness, low cost, simple preparation and use method, and the like.
Description
Technical field
The utility model is a kind of Waveguide coupling metal photonic crystal biosensor, its based on Physical Mechanism be strong spectroscopy coupling between particle plasma resonance in the metal photonic crystal and Wave guide resonance pattern, can be used for the concentration of biomolecule (protein, carbohydrate molecule, DNA etc.) and the high sensitivity sensing of bioactive molecule idiosyncrasy, belong to the crossing domain of photoelectron technology and biotechnology.
Background technology
The biomolecule sensor of high sensitivity, high-resolution characteristic plays important effect in scientific researches such as biology, life science, medical science and practical application.Show special advantages based on the biology sensor of spectroscopy response characteristic owing to its reliable physics principle and accurate technological means.
The basic structure of traditional surface plasma resonance (SPR) biology sensor as shown in Figure 1.This sensor is by transparency carrier 1, and evaporation is at the metal level 2 of substrate 1 upper surface, prism 6, and light source 7, photodetector 8 constitutes.It is such that surface plasmon resonance biosensor is used for biospecific reaction sensing principle of work: the light beam from wideband light source 7 incides prism 6 at a certain angle, prism 6 imports transparency carrier with incident light with wide-angle, and the incident light that is imported into total reflection takes place and excite evanescent wave (Evanescent Wave) in metal level 2 at the interface of metal level 2 and substrate 1.Under given incident angle situation, a certain frequency in the evanescent wave will reach consistent with the surface plasma resonance frequency of metal level 2, make incident light in the strong resonance absorption of this frequency quilt, record the significant absorption peak in this frequency place in its reflectance spectrum.Here it is, and so-called surface plasma resonance absorbs.In actual applications, also can adopt monochromatic source (as laser), by changing the purpose that incident angle realizes that metal level absorbs its surface plasma resonance as incident light.Currently used technology mostly is the former, promptly adopts the mode that is fixed into firing angle and wideband light source, problems such as complex operation, the error that can avoid angular adjustment to cause is big, system's instability.
In the bio-sensing experiment, at first can be fixed on the metal level 2 with the acceptor 4 of part 3 generation specific recognition reactions, because the surface plasma resonance frequency depends on the specific inductive capacity (refractive index) of environment consumingly, the metallic film after the acceptor molecule is fixing has possessed specific SPR frequency.The acceptor and the part in the sample solution 53 that will be fixed on metallic film 2 then interact, acceptor and part generation specific reaction, variation has just taken place in the specific inductive capacity at layer on surface of metal place, thereby causes the surface plasma resonance frequency of metal level to be offset.Thus, can obtain ligand concentration information in the sample solution.
Biology sensor based on the SPR technology still has the device architecture complexity, the technology of preparing requirement is high, system operation is loaded down with trivial details, test period is long, thus shortcomings such as cost height.Therefore, biology sensor simple in structure, highly sensitive, that test process is quick, cost is low becomes an urgent demand and the important research and development content in fields such as biomedicine.
The utility model content
The purpose of this utility model has been to overcome the above-mentioned defective of existing biology sensor, has proposed a kind of Waveguide coupling metal photonic crystal biosensor, advantage such as that this biology sensor has is highly sensitive, cost is low, preparation and using method are simple.
Biology sensor in the utility model based on Physical Mechanism be: strong coupling between particle plasma resonance (PPR) pattern of Wave guide resonance pattern (WGM) and metal photonic crystal, so be called " Waveguide coupling metal photonic crystal biosensor ".
Technical solution adopted in the utility model is as follows: this biology sensor comprises substrate 21 from top to bottom successively, covers ducting layer transparent in the substrate 21 22, covers the metal photonic crystal 23 on the ducting layer 22.
The thickness of described ducting layer 21 is 60nm～300nm.
Described metal photonic crystal 23 is one-dimensional metal photon crystals or two-dimensional metallic photonic crystal.
The cycle of described one-dimensional metal photon crystals is 150nm～550nm.
The cycle of described two-dimensional metallic photonic crystal both direction can be the same or different, and span is 150nm～550nm.
Use above-mentioned Waveguide coupling metal photonic crystal biosensor to detect, its detection method is as follows:
1) after fixing the acceptor 4 that part 3 is had the specific recognition function on the metal photonic crystal 23, the surface of blank sample solution with metal photonic crystal 23 contacted;
2) become θ angle (scope of θ is 0～80 °) irradiation metal photonic crystal, the extinction spectra that photodetector 8 detects through the transmitted light of blank sample solution, metal photonic crystal 23, ducting layer 22 and substrate 21 with normal behind the light process Polarization Controller 31 that light source 7 sends with plane, substrate 21 place;
3) sample solution that will contain part surface, part and the acceptor generation specific reaction of metal photonic crystal 23 of flowing through.After utilizing blank sample solution in the step 1) to wash the part that does not react, again the surface of blank sample solution with metal photonic crystal 23 contacted;
4) light that sends of light source 7 through Polarization Controller 31 backs to become and step 2 with the normal on plane, substrate 21 place) identical θ angular illumination metal photonic crystal, the extinction spectra that photodetector 8 detects through the transmitted light of blank sample solution, metal photonic crystal, ducting layer 22 and substrate 21;
5) with step 2) do the calculating of secondary extinction spectrum with the extinction spectra in the step 4), by characterizing the spectroscopy Changing Pattern of different ligands concentration, realize detection by quantitative to ligand concentration.
Use above-mentioned Waveguide coupling metal photonic crystal biosensor to detect, also can detect as follows:
1) after fixing the acceptor 4 that part 3 is had the specific recognition function on the metal photonic crystal 23, the surface of blank sample solution with metal photonic crystal 23 contacted;
2) become θ angle (scope of θ is 0～80 °) irradiation substrate with the normal on plane, substrate 21 place behind the light process Polarization Controller 31 that light source 7 sends, photodetector 8 detects the extinction spectra of the transmitted light of process substrates 21, ducting layer 22, metal photonic crystal 23 and blank sample solution;
3) sample solution that will contain part surface, part and the acceptor generation specific reaction of metal photonic crystal 23 of flowing through.Utilize the blank sample solution of step 1) to wash the part that does not react, at last blank sample solution is contacted with the surface of metal photonic crystal 23 again;
4) become and step 2 with the normal on plane, substrate 21 place behind the light process Polarization Controller 31 that light source 7 sends) identical θ angular illumination substrate, photodetector 8 detects the extinction spectra of the reacted transmitted light of process substrates 21, ducting layer 22, metal photonic crystal 23 and blank sample solution;
5) with step 2) do the calculating of secondary extinction spectrum with the extinction spectra in the step 4), by characterizing the spectroscopy Changing Pattern of different ligands concentration, realize detection by quantitative to ligand concentration.
Use above-mentioned Waveguide coupling metal photonic crystal biosensor to detect, also can detect as follows:
1) after fixing the acceptor 4 that part 3 is had the specific recognition function on the metal photonic crystal 23, the surface of blank sample solution with metal photonic crystal 23 contacted;
2) become θ angle (scope of θ is 0～80 °) irradiation metal photonic crystal with the normal on plane, substrate 21 place behind the light process Polarization Controller 31 that light source 7 sends, photodetector 8 detects the catoptrical extinction spectra through being fired back by substrate 21 behind blank sample solution, metal photonic crystal and the ducting layer 22 successively;
3) sample solution that will contain part surface, part and the acceptor generation specific reaction of metal photonic crystal 23 of flowing through.Utilize the blank sample solution of step 1) to wash the part that does not react, at last blank sample solution is contacted with the surface of metal photonic crystal 23 again.
4) light that sends of light source 7 becomes and step 2 with the normal on plane, substrate 21 place through Polarization Controller 31 backs) identical θ angular illumination metal photonic crystal, the catoptrical extinction spectra through being reflected by substrate 21 behind blank sample solution, metal photonic crystal 23 and the ducting layer 22 successively after photodetector 8 detection reaction;
5) with step 2) do the calculating of secondary extinction spectrum with the extinction spectra in the step 4), by characterizing the spectroscopy Changing Pattern of different ligands concentration, realize detection by quantitative to ligand concentration.
Use above-mentioned Waveguide coupling metal photonic crystal biosensor to detect, also can detect as follows:
1) after fixing the acceptor 4 that part 3 is had the specific recognition function on the metal photonic crystal 23, the surface of blank sample solution with metal photonic crystal 23 contacted;
2) become θ angle (scope of θ is 0～80 °) irradiation substrate 21 with the normal on plane, substrate 21 place behind the light process Polarization Controller 31 that light source 7 sends, photodetector 8 detects the catoptrical extinction spectra through being reflected by metal photonic crystal behind substrate 21 and the ducting layer 22 successively;
3) sample solution that will contain part surface, part and the acceptor generation specific reaction of metal photonic crystal 23 of flowing through.Utilize the blank sample solution of step 1) to wash the part that does not react, at last the surface of blank sample solution with metal photonic crystal 23 contacted.
4) become and step 2 with the normal on plane, substrate 21 place behind the light process Polarization Controller 31 that light source 7 sends) identical θ angular illumination substrate 21, after passing through substrate 21 and ducting layer 22 after photodetector 8 detection reaction successively, the catoptrical extinction spectra that is reflected by metal photonic crystal;
5) with step 2) do the calculating of secondary extinction spectrum with the extinction spectra in the step 4), by characterizing the spectroscopy Changing Pattern of different ligands concentration, realize detection by quantitative to ligand concentration.
Device in the utility model can also be used to the variation of detection by quantitative material concentration, and following several detection method is specifically arranged:
One, use the Waveguide coupling metal photonic crystal biosensor in the utility model to carry out the detection of material concentration, can carry out as follows:
1) surface of blank sample solution with metal photonic crystal 23 contacted;
2) become θ angle (scope of θ is 0～80 °) irradiation metal photonic crystal with the normal on plane, substrate 21 place behind the light process Polarization Controller 31 that light source 7 sends, photodetector 8 detects the extinction spectra of the transmitted light of the blank sample solution of process, metal photonic crystal 23, ducting layer 22 and substrate 21;
3) sample solution that will contain certain material surface of metal photonic crystal 23 of flowing through, and contact with the surface of metal photonic crystal 23.
4) become and step 2 with the normal on plane, substrate 21 place behind the light process Polarization Controller 31 that light source 7 sends) identical θ angular illumination metal photonic crystal, photodetector 8 detects the extinction spectra of the transmitted light of process sample solutions, metal photonic crystal 23, ducting layer 22 and substrate 21;
5) with step 2) do the calculating of secondary extinction spectrum with the extinction spectra in the step 4), by characterizing the spectroscopy Changing Pattern of this material variable concentrations, realize detection by quantitative to this material concentration.
Two, the difference of this method and method one is to detect light from substrate incident, and concrete steps are as follows:
1) surface of blank sample solution with metal photonic crystal 23 contacted;
2) become θ angle (scope of θ is 0～80 °) irradiation substrate 21 with the normal on plane, substrate 21 place behind the light process Polarization Controller 31 that light source 7 sends, photodetector 8 detects the extinction spectra of the transmitted light of process substrates 21, ducting layer 22, metal photonic crystal 23 and blank sample solution;
3) sample solution that will contain certain material surface of metal photonic crystal 23 of flowing through, and contact with the surface of metal photonic crystal 23.
4) become and step 2 with the normal on plane, substrate 21 place behind the light process Polarization Controller 31 that light source 7 sends) identical θ angular illumination substrate 21, photodetector 8 detects the extinction spectra of the transmitted light of process substrates 21, ducting layer 22, metal photonic crystal 23 and sample solution;
5) with step 2) do the calculating of secondary extinction spectrum with the extinction spectra in the step 4), by characterizing the spectroscopy Changing Pattern of this material variable concentrations, realize detection by quantitative to this material concentration.
Three, the method for the detection of reflected light of this method employing is determined the variation of material concentration, and its concrete steps are as follows:
1) surface of blank sample solution with metal photonic crystal 23 contacted;
2) become θ angle (scope of θ is 0～80 °) to shine metal photonic crystal with the normal on plane, substrate 21 place behind the light process Polarization Controller 31 that light source 7 sends, after photodetector 8 detects and passes through blank sample solution, metal photonic crystal 23 and ducting layer 22 successively, the catoptrical extinction spectra that is reflected by substrate 21;
3) sample solution that will contain certain material surface of metal photonic crystal 23 of flowing through, and contact with the surface of metal photonic crystal 23.
4) become and step 2 with the normal on plane, substrate 21 place behind the light process Polarization Controller 31 that light source 7 sends) identical θ angular illumination metal photonic crystal, after photodetector 8 detects and passes through sample solution, metal photonic crystal and ducting layer 22 successively, the catoptrical extinction spectra that is reflected by substrate 21;
5) with step 2) do the calculating of secondary extinction spectrum with the extinction spectra in the step 4), by characterizing the spectroscopy Changing Pattern of this material variable concentrations, realize detection by quantitative to this material concentration.Four, the difference of this method and method three is to detect light from substrate incident, and concrete steps are as follows:
1) surface of blank sample solution with metal photonic crystal 23 contacted;
2) become θ angle (scope of θ is 0～80 °) irradiation substrate 21 with the normal on plane, substrate 21 place behind the light process Polarization Controller 31 that light source 7 sends, photodetector 8 detects the catoptrical extinction spectra that is reflected by metal photonic crystal through behind substrates 21 and the ducting layer 22;
3) sample solution that will contain certain material surface of metal photonic crystal 23 of flowing through, and contact with the surface of metal photonic crystal 23;
4) light that sends of light source 7 becomes and step 2 with the normal on plane, substrate 21 place through Polarization Controller 31 backs) identical θ angular illumination substrate 21, photodetector 8 detects the catoptrical extinction spectra through being reflected by metal photonic crystal behind substrate 21 and the ducting layer 22 successively;
5) with step 2) do the calculating of secondary extinction spectrum with the extinction spectra in the step 4), by characterizing the spectroscopy Changing Pattern of this material variable concentrations, realize detection by quantitative to this material concentration.
The principle of work of this biology sensor is as shown in Figure 5: incident light 24 excited particles plasma resonance excimer and when the characteristic spectrum district is by strong absorption, excite waveguide propagation modes 27 by the metal photonic crystal diffraction and in ducting layer 22 in metal photonic crystal 23.Produced the diffraction light 28 that multi beam is parallel to transmitted light 26 by the metal photonic crystal diffraction of upper surface in the waveguide mode communication process, diffraction light 28 further interferes with transmitted light 26.Like this, can observe the transmission enhancing signal of an arrowband in the broadband of the transmitted light that records etc. in resonance absorption spectrum, this is the result of waveguide mode and the effect of plasma resonance Mode Coupling.The particle plasma resonance of metal photonic crystal absorbs and the spectroscopy response characteristic of Wave guide resonance pattern all is offset with metal photonic crystal upper surface environment change of refractive, and its side-play amount is directly corresponding to environment change of refractive amount.Therefore, the particle plasma resonance absorbs and the variation of the coupling spectrum of Wave guide resonance pattern can quantitatively characterizing environment change of refractive amount by measuring.The principle of work of biology sensor in Here it is the utility model.Wherein the introducing of arrowband Wave guide resonance pattern has improved the sensitivity of sensor response greatly.
The detection principle of Waveguide coupling metal photonic crystal biosensor of the present utility model is as follows: in biospecific reaction sensing experiment, at first will be fixed on the surface of metal photonic crystal 23, constitute the initial diaphragm of sensor the acceptor (antibody) 4 that part (antigen) 3 has a specific recognition function; With the flow through surface of metal photonic crystal 23 of sample solution 5, if have part (antigen) 3 in the test portion, specific reaction just can take place with part (antigen) 3 in acceptor (antibody) 4, variation according to extinction spectra before and after the reaction, quantitatively provide the subtle change of coupling between waveguide mode and particle plasma resonance pattern, by quantitatively characterizing, realize detection to the concentration of part (antigen) 3 in the sample solution 5 to the spectrum change rule.In the material concentration test experience, the flow through surface of metal photonic crystal 23 of sample solution 5, if the concentration of test substance changes in the test portion, variation according to extinction spectra before and after the concentration change, quantitatively provide the subtle change of coupling between waveguide mode and particle plasma resonance pattern, by quantitatively characterizing, realize detection to material concentration to be measured to the spectrum change rule.
The advantageous feature that the utility model is different from traditional spr sensor embodies in the following areas:
1) cost is low: no matter be that Waveguide coupling metal photonic crystal senser element or the cost of spectroscopy test macro are all far below the spr sensor system.The whole sensor system cost has only below 10% of existing in the market surface plasmon resonance biosensor price.The Waveguide coupling metal photonic crystal sensor of its core can be reused, and has improved the dirigibility of practical application.
2) highly sensitive: the introducing of Wave guide resonance pattern has realized the narrowband modulation to particle plasma resonance pattern, has improved the sensitivity of device to the response of environment variations in refractive index greatly.
3) means of testing is flexible, easy: according to different demands, various test is available; In the test process, only need to adopt conventional optics and spectroscopy means of testing, obtain the extinction spectra of transmission or reflectance spectrum or transmission or reflection.
4) be convenient to integrated: the Waveguide coupling metal photonic crystal volume is little, and regular shape is easy to embedded system, constitutes the biochip structure.
Description of drawings
The surface plasma resonance biological sensor structural representation that Fig. 1 is traditional
The sectional view of Fig. 2 Waveguide coupling metal photonic crystal biosensor of the present utility model
The front elevation of Fig. 3 waveguide-coupled one-dimensional metal photon crystals of the present utility model biology sensor
The front elevation of Fig. 4 waveguide-coupled two-dimensional metallic photonic crystal of the present utility model biology sensor
The optical schematic diagram of Fig. 5 Waveguide coupling metal photonic crystal biosensor of the present utility model
The structural drawing of Fig. 6 Waveguide coupling metal photonic crystal biosensor of the present utility model
The positive transmission-type of Fig. 7 biology sensor of the present utility model detects synoptic diagram
The back side transmission-type of Fig. 8 biology sensor of the present utility model detects synoptic diagram
The positive reflective detection synoptic diagram of Fig. 9 biology sensor of the present utility model
The backside reflection formula of Figure 10 biology sensor of the present utility model detects synoptic diagram
The extinction spectra of the transmission-type waveguide-coupled gold photonic crystal biosensor in Figure 11 the utility model
The detection signal of the transmission-type waveguide-coupled gold photonic crystal biosensor in Figure 12 the utility model
The catoptrical extinction spectra of the reflective waveguide-coupled gold photonic crystal biosensor in Figure 13 the utility model
The detection signal of the reflective waveguide-coupled gold photonic crystal biosensor in Figure 14 the utility model
Among the figure: 1. transparency carrier, 2. metal level, 3. part (antigen), 4. acceptor (antibody), 5. sample solution, 6. prism, 7. light source, 8. photodetector, 21. substrates, 22. ducting layers, 23. metal photonic crystals, 31. Polarization Controllers.
Embodiment
The utility model is described in further detail with reference to the accompanying drawings:
Biology sensor in the utility model comprises substrate 21 (thickness is D), cover the transparent ducting layer 22 (thickness d) in the substrate 21 and cover metal photonic crystal 23 (cycle is Λ) on the ducting layer 22, and the sectional view of this biology sensor as shown in Figure 2.Wherein: the span of the thickness d of ducting layer 22 is 60～300nm, and metal photonic crystal 23 can adopt one dimension (Fig. 3) or two-dimensional structure (Fig. 4), is called one dimension or two-dimensional metallic photonic crystal.The cycle of one-dimensional metal photon crystals is 150～550nm, and the cycle of 2 D photon crystal both direction all can be taken as 150～550nm.
Biology sensor in the utility model prepares metal (preferred gold, silver or platinum) one dimension or 2 D photon crystal on the transparent waveguide layer 22 of substrate 21, obtain as Fig. 2～Waveguide coupling metal photonic crystal biosensor shown in Figure 4;
The detection method of the Waveguide coupling metal photonic crystal biosensor in the utility model is as follows:
1) in biologic specificity identification detects, at first will be fixed on the surface of metal photonic crystal 23 in the Waveguide coupling metal photonic crystal biosensor for preparing in the step 1), constitute the initial diaphragm of sensor the acceptor (antibody) 4 that part (antigen) 3 has a specific recognition function;
2) the spectrum test method can adopt as Fig. 7～mode shown in Figure 10:
3) sample solution 5 the is flowed through surface of the biology sensor that step 3) obtains, if have part (antigen) 3 in the test portion, acceptor (antibody) 4 just with its generation specific reaction, variation according to extinction spectra before and after the reaction, quantitatively provide the subtle change (as Figure 11 or shown in Figure 13) of coupling between waveguide mode and particle plasma resonance pattern, by characterizing the spectroscopy Changing Pattern of variable concentrations antigen, realize detection by quantitative to part (antigen) 3 concentration in the sample solution 5.
The detection of the Waveguide coupling metal photonic crystal biosensor in the utility model also can be adopted following method:
1) sample solution (solvent) with blank contacts with the surface of metal photonic crystal 23;
2) the spectrum test method can adopt as Fig. 7～mode shown in Figure 10;
3) sample solution (solution of test substance) that will contain certain material contacts with the surface of metal photonic crystal 23, variation according to sample solution concentration, quantitatively provide the subtle change (as Figure 11 or shown in Figure 13) of coupling between waveguide mode and particle plasma resonance pattern, by characterizing the spectroscopy Changing Pattern of this material variable concentrations, realize detection by quantitative to this material concentration;
Be the device in the utility model and the example of detection method thereof below:
Embodiment 1:
Present embodiment is positive transmission-type waveguide-coupled one-dimensional metal photon crystals biology sensor and detection method thereof:
The glass of thickness D=1mm is adopted in employed substrate 21 in the present embodiment, and ducting layer 22 adopts tin indium oxide (ITO) film, and thickness is the glass sheet of d=200nm.
It is Λ=sub-crystal of 330nm one dimension golden light that present embodiment adopts preparation method's manufacturing cycle on the ITO of substrate of glass ducting layer of interference lithography binding soln method, obtains waveguide-coupled one dimension gold photonic crystal biosensor.When utilizing this sensor to detect, its detection method is as follows:
1) will be fixed on the surface of the metal photonic crystal 23 in the waveguide-coupled one-dimensional metal photon crystals biology sensor to the acceptor (antibody) 4 that part (antigen) 3 has a specific recognition function, constitute the initial diaphragm of sensor, blank sample solution (buffer solution or the solvent that do not contain test substance) is contacted with the surface of metal photonic crystal 23;
2) the spectrum test mode adopts positive transmission-type as shown in Figure 7, light source is selected bromine tungsten filament lamp for use, incident light becomes waveguide-coupled one-dimensional metal photon crystals irradiates light in ° angle present embodiment of θ=32 through Polarization Controller 31 with the normal with plane, substrate 21 place, the extinction spectra that photodetector 8 detects through the transmitted light of blank sample solution, one-dimensional metal photon crystals, ducting layer and substrate;
3) will contain sample solution (buffer solution or the solvent that the contain test substance) step 2 of flowing through of part (antigen) 3) surface of the biology sensor that obtains, the specific recognition reaction takes place with acceptor (antibody) 4 in part (antigen) 3, utilize step 2) blank sample solution wash the antigen that does not react, at last the surface of blank sample solution with metal photonic crystal 23 contacted;
4) behind the light process Polarization Controller 31 that the bromine tungsten filament lamp light source sends, become θ=32 ° angular illumination one-dimensional metal photon crystals with the normal with plane, substrate 21 place, photodetector detects the extinction spectra through transmitted light after the reaction of blank sample solution, one-dimensional metal photon crystals, ducting layer and substrate;
5) step 2) with step 4) in extinction spectra relatively, the subtle change of coupling between waveguide mode and particle plasma resonance pattern before and after the acquisition reaction as shown in figure 11, extinction spectra is made the secondary extinction spectral manipulation, obtain signal as shown in figure 12, by characterizing the spectroscopy Changing Pattern of different antigen concentrations, realize detection by quantitative to antigen concentration.
Embodiment 2:
Present embodiment is back side transmission-type two-dimensional waveguide coupling metal photonic crystal biosensor and device detection method:
The glass of thickness D=1mm is adopted in employed substrate in the present embodiment, and ducting layer adopts ITO, and thickness is the glass sheet of d=200nm.
Present embodiment adopts the preparation method of interference lithography binding soln method prepare the both direction cycle on the ITO of substrate of glass ducting layer and is Λ=sub-crystal of 350nm two dimension golden light, obtains the two-dimentional golden photonic crystal biosensor of waveguide-coupled.When utilizing this sensor to detect, its detection method is as follows:
1) will be fixed on the surface of metal photonic crystal in the two-dimensional waveguide coupling metal photonic crystal biosensor to the acceptor (antibody) 4 that part (antigen) 3 has a specific recognition function, constitute the initial diaphragm of sensor, blank sample solution is contacted with the surface of metal photonic crystal;
2) the spectrum test mode adopts the back side (basal surface) transmission-type as shown in Figure 8, light source is selected bromine tungsten filament lamp for use, incident light is through becoming the substrate irradiation of the waveguide-coupled two-dimensional metallic photonic crystal biology sensor in ° angle present embodiment of θ=48 with the normal with plane, substrate 21 place behind the Polarization Controller, photodetector detects the extinction spectra through the transmitted light of substrate, ducting layer, two-dimensional metallic photonic crystal and blank sample solution;
3) sample solution that will the contain antigen step 2 of the flowing through) surface of the biology sensor that obtains, antigen and the reaction of antibody generation specific recognition, utilize step 2) blank sample solution wash the antigen that does not react, at last the surface of blank sample solution with metal photonic crystal 23 contacted.
4) behind the light process Polarization Controller that the bromine tungsten filament lamp light source sends, become θ=48 ° angular illumination substrate with the normal with plane, substrate 21 place, photodetector detects the extinction spectra of incident light through the transmitted light of substrate, ducting layer, two-dimensional metallic photonic crystal and blank sample solution;
5) step 2) with step 4) in extinction spectra relatively, obtain as shown in figure 11 waveguide mode and particle plasma resonance pattern between the subtle change of coupling, extinction spectra is made the secondary extinction spectral manipulation, obtain signal as shown in figure 12, by characterizing the spectroscopy Changing Pattern of different antigen concentrations, realize detection by quantitative to antigen concentration.
Embodiment 3:
Present embodiment is positive reflective waveguide-coupled one-dimensional metal photon crystals biology sensor and detection method thereof:
The glass of thickness D=1mm is adopted in employed substrate in the present embodiment, and ducting layer adopts ITO, and thickness is the glass sheet of d=210nm.
It is Λ=sub-crystal of 400nm one dimension golden light that present embodiment adopts preparation method's manufacturing cycle in the ITO of substrate of glass waveguide of interference lithography binding soln method, obtains waveguide-coupled one dimension gold photonic crystal biosensor;
1) will be fixed on the surface of metal photonic crystal in the waveguide-coupled one-dimensional metal photon crystals biology sensor to the antibody that antigen has a specific recognition function, constitute the initial diaphragm of sensor, the surface of blank sample solution with metal photonic crystal 23 contacted;
2) the spectrum test mode adopts as shown in Figure 9 positive reflective, light source is selected bromine tungsten filament lamp for use, incident light is through becoming the one-dimensional metal photon crystals face irradiation of the waveguide-coupled one-dimensional metal photon crystals biology sensor in ° angle present embodiment of θ=2 with the normal with plane, substrate 21 place behind the Polarization Controller, through being reflected by substrate 21 behind blank sample solution, one-dimensional metal photon crystals, the ducting layer, photodetector 8 receives this catoptrical extinction spectra to irradiates light successively;
3) sample solution that will the contain antigen step 2 of the flowing through) surface of the biology sensor that obtains, antigen and the reaction of antibody generation specific recognition, utilize step 2) blank sample solution wash the antigen that does not react, at last the surface of blank sample solution with metal photonic crystal 23 contacted.
4) behind the light process Polarization Controller that the bromine tungsten filament lamp light source sends, become θ=2 ° angular illumination one-dimensional metal photon crystals with normal with plane, substrate 21 place, photodetector detects through behind blank sample solution, one-dimensional metal photon crystals, the ducting layer, the reacted catoptrical extinction spectra that is reflected by substrate;
5) step 2) with step 4) in extinction spectra relatively, the subtle change of coupling between waveguide mode and particle plasma resonance pattern before and after the acquisition reaction as shown in figure 13, extinction spectra is made the secondary extinction spectral manipulation, obtain signal as shown in figure 14, by characterizing the spectroscopy Changing Pattern of different antigen concentrations, realize detection by quantitative to antigen concentration.
Embodiment 4:
Present embodiment is backside reflection formula one-dimensional wave guide coupling metal photonic crystal biosensor and detection method thereof: the device in the present embodiment is identical with embodiment 3, its detection method is substantially the same manner as Example 3, difference only is: incident light is through behind the Polarization Controller, incide in the substrate 21, what detecting device 8 detected is that incident light is behind substrate, ducting layer, metal photonic crystal, by the catoptrical extinction spectra of metal photonic crystal.
Sensor in the utility model also can be realized the detection to material concentration, describes below in conjunction with concrete detection method:
Embodiment 5:
Present embodiment is positive transmission-type two-dimensional waveguide coupling metal photonic crystal biosensor and device detection method:
The glass of thickness D=1mm is adopted in employed substrate in the present embodiment, and ducting layer adopts ITO, and thickness is the glass sheet of d=200nm.
Present embodiment adopts the preparation method of interference lithography binding soln method prepare the both direction cycle on the ITO of substrate of glass ducting layer and is Λ=sub-crystal of 350nm two dimension golden light, obtains the two-dimentional golden photonic crystal biosensor of waveguide-coupled.Device in the use present embodiment is to the detection of material concentration, and the step of its detection is as follows:
1) blank test portion (pure water) is contacted with the surface of two-dimensional metallic photonic crystal 23;
2) become 36 ° of angles irradiation metal photonic crystals with the normal on plane, substrate 21 place behind the light process Polarization Controller 31 that light source 7 sends, photodetector 8 detects the extinction spectra of the transmitted light that passes through pure water, metal photonic crystal 23 ducting layers 22 and substrate 21 successively;
3) sample solution that will contain sucrose flows (aqueous solution of sucrose) through the surface of metal photonic crystal 23, and contacts with the surface of metal photonic crystal 23;
4) become 36 ° of angles irradiation metal photonic crystals with the normal on plane, substrate 21 place behind the light process Polarization Controller 31 that light source 7 sends, photodetector 8 detects the extinction spectra of the transmitted light that passes through sucrose solution, metal photonic crystal 23, ducting layer 22 and substrate 21 successively;
5) with step 2) do the calculating of secondary extinction spectrum with the extinction spectra in the step 4), obtain signal as shown in figure 14 because of sucrose concentration variation generation, by characterizing the spectroscopy Changing Pattern of variable concentrations sucrose, realization is to the detection by quantitative of sucrose concentration.
Embodiment 6:
Present embodiment is back side transmission-type one-dimensional wave guide coupling metal photonic crystal biosensor and detection method thereof: the device in the present embodiment is identical with embodiment 5, its detection method is substantially the same manner as Example 5, difference only is: incident light is through behind the Polarization Controller, incide in the substrate 21, what photodetector 8 detected is the extinction spectra of incident light transmitted light behind substrate, ducting layer, metal photonic crystal.
Embodiment 7:
Present embodiment is positive reflective one-dimensional wave guide coupling metal photonic crystal biosensor and detection method thereof: the device in the present embodiment is identical with embodiment 5, its detection method is substantially the same manner as Example 5, difference only is: incident light is through behind the Polarization Controller, incide on the metal photonic crystal 23, what detecting device 8 detected is that incident light is after metal photonic crystal, ducting layer, substrate, by the catoptrical extinction spectra of substrate 21 reflections.
Embodiment 8:
Present embodiment is backside reflection formula one-dimensional wave guide coupling metal photonic crystal biosensor and detection method thereof: the device in the present embodiment is identical with embodiment 5, its detection method is substantially the same manner as Example 5, difference only is: incident light is through behind the Polarization Controller, incide in the substrate 21, what photodetector 8 detected is after incident light passes through substrate, ducting layer, metal photonic crystal successively, the catoptrical extinction spectra that is reflected by metal photonic crystal 21.
Claims (4)
1, Waveguide coupling metal photonic crystal biosensor is characterized in that: comprise substrate from top to bottom successively, cover suprabasil transparent ducting layer and cover metal photonic crystal on the ducting layer.
2, Waveguide coupling metal photonic crystal biosensor according to claim 1 is characterized in that: the thickness of described ducting layer is 60nm～300nm.
3, Waveguide coupling metal photonic crystal biosensor according to claim 1 is characterized in that: described metal photonic crystal is one-dimensional metal photon crystals or two-dimensional metallic photonic crystal.
4, Waveguide coupling metal photonic crystal biosensor according to claim 3 is characterized in that: the cycle of described one-dimensional metal photon crystals is 150nm～550nm; The cycle of described two-dimensional metallic photonic crystal both direction is 150nm～550nm.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105538812A (en) * | 2015-12-11 | 2016-05-04 | 深圳大学 | High-sensitivity sensing film and surface plasmon resonance sensing detection system |
| CN107991233A (en) * | 2017-11-02 | 2018-05-04 | 宁波大学 | Noble metal nano array extinction spectra measuring device and its sensing detection method |
| CN108732138A (en) * | 2017-04-15 | 2018-11-02 | 大连理工大学 | A kind of super clever surface biological sensor of photon |
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2009
- 2009-03-06 CN CN 200920106042 patent/CN201434868Y/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105538812A (en) * | 2015-12-11 | 2016-05-04 | 深圳大学 | High-sensitivity sensing film and surface plasmon resonance sensing detection system |
| CN108732138A (en) * | 2017-04-15 | 2018-11-02 | 大连理工大学 | A kind of super clever surface biological sensor of photon |
| CN107991233A (en) * | 2017-11-02 | 2018-05-04 | 宁波大学 | Noble metal nano array extinction spectra measuring device and its sensing detection method |
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