CN102967593A - Method of optical waveguide enhancement mechanism and Raman spectrometer - Google Patents
Method of optical waveguide enhancement mechanism and Raman spectrometer Download PDFInfo
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- CN102967593A CN102967593A CN2012104931412A CN201210493141A CN102967593A CN 102967593 A CN102967593 A CN 102967593A CN 2012104931412 A CN2012104931412 A CN 2012104931412A CN 201210493141 A CN201210493141 A CN 201210493141A CN 102967593 A CN102967593 A CN 102967593A
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Abstract
The invention relates to a design concept of Raman spectrum of an optical waveguide enhancement mechanism, and the design concept of the Raman spectrum of the optical waveguide enhancement mechanism is used for identifying substance and qualitatively and quantitatively analyzing property of the substance. A Raman spectrometer consists of a metal film, thick glasses, a sample cell and a metal film, and is capable of directly coupling a laser in a wavelength of 785 nm into a waveguide layer between two metal layers to generate an ultrahigh-order guide mode so as to enhance Raman scattering, wherein the ultrahigh-order guide mode has an ultrahigh light intensity and is irrelevant with polarization. The enhancement is different from surface plasma enhancement, and metal nanometer sol is unnecessary to be added; and the enhancement is also different from a resonance Raman effect and liquid core fiber Raman enhancement, and has no requirement on wavelength of an incident light and refractive index of a sample.
Description
Technical field
The present invention relates to the new method that a kind of Raman spectrum strengthens, particularly utilize the mm-scale double-sided metal to coat waveguide and realize the new method that the biological sample Raman spectrum strengthens in conjunction with free-space coupling technology.
Background technology
Raman spectrum belongs to molecular vibration spectrum, can be used for differentiating the character of material, qualitative and quantitative analysis material, and purposes is very extensive.But raman spectral signal is very faint, only is 10 of incident intensity generally
-6～10
-10The method that strengthens at present Raman spectrum mainly contains three kinds: the first is Surface enhanced raman spectroscopy (SERS), namely utilize metal surface local plasmon excimer to excite caused Electromagnetic enhancement, make the Raman scattering of determinand produce enhancement effect, enhancer can reach 10
6, even higher, SERS makes the application of Raman spectrum enter the unimolecule field, and shortcoming is to need sample is done pre-service before the SERS technology test sample, namely measured object absorption or near metal or nano-colloid surface; Secondly, the alkaline metal (such as lithium, sodium etc.) and a series of pure transition metal that only have gold, silver, three kinds of metals of copper and the minority utmost point to be of little use have strong SERS effect.The second Enhancement Method is to utilize resonance Raman effect (RR), when selecting laser wavelength of incidence corresponding to the Electron absorption energy level of molecule, the probability of Raman transition increases greatly, so that the raman scattering cross section of some vibration mode of molecule strengthens, resonance strengthens can obtain 10
3-10
5Enhancer, make the molecule that detects inferior individual layer amount become possibility, can be used for low concentration and micro-example and detect, such as the detection of biomacromolecule sample, but only have at present the Electron absorption energy level of a few molecules to be complementary with existing laser.The third is to adopt liquid-core optical fibre (LCOF) system to carry out Raman spectrum to strengthen, and this method has increased the interaction between light and matter distance greatly, thereby has significantly improved spectral signal intensity and detection limit.But the LCOF method requires the refractive index of fluid sample must be greater than the refractive index of fibre cladding, and the refractive index of most of liquid all the refractive index than conventional fiber covering is little, significant limitation is arranged in the practical application.
Because Raman spectrum can be from molecular level reflection tissue, the difference of cell on chemical constitution and molecular structure, the structure of large molecule such as albumen, nucleic acid, ester class etc. and the finger print information of structure can be provided, and the great potential of Raman spectrum in clinical disease monitoring and early diagnosis caused various countries scientists' broad interest and concern.Bibliographical information adopt common Raman or Raman to strengthen method study the result of study with obvious characteristic difference of multiple cancerous issue such as lung cancer, breast cancer, carcinoma of urinary bladder, prostate cancer, the cancer of the uterus, laryngocarcinoma, nasopharyngeal carcinoma and cutaneum carcinoma etc. and the Raman spectrum of normal structure, the linear relationship between the concentration of raman scattering intensity and human metabolite such as glucose, lactose, urea, cholesterol and triglyceride etc. has been set up in multinomial research, except cancer, Raman spectrum also is used for studying diabetes, atherosclerotic and old syndrome etc.These achievements in research show that the utmost point is hopeful to carry out rapidly disease surveillance and diagnosis by the Raman spectrum of the biological samples such as direct measurement blood of human body, urine and cell in the future, and the method for the highly sensitive high-level efficiency Raman Measurement of postgraduate's matter sample is significant.
Summary of the invention
The present invention is directed to traditional Raman spectrum and the existing deficiency that strengthens Raman optical spectrum method, utilize the mm-scale double-sided metal to coat the waveguide platform, realize that in conjunction with free-space coupling technology the Raman of biological sample strengthens.The Raman detection device cross-section structure that coats waveguide based on double-sided metal as shown in Figure 1, wherein the double layer of metal film is the overlayer of waveguide up and down, as the glass sheet of upper strata metal film substrate and the ducting layer of sample chamber formation waveguide, minute subsample for flowing in the sample chamber, laser can directly be incident in upper metallic film surface from free space, satisfy the light beam of vowing matching condition and will be coupled into waveguide, become the Ultra-High Order guided mode in the waveguide, with sample molecule generation scattering in the sample cell, the Raman probe of reflected light direction receives aforementioned Raman diffused light.
Inject biological sample in the described sample cell, chemical solution or material, the wave band that the optical responsivity of sample is large is determined the thickness of upper strata metallic film.
Described sample cell is millimetre-sized cavity, and two ends respectively connect a passage, and sample can be injected in passage two, realizes Real-Time Monitoring.
Described side Raman probe comprises a notch filter sheet that is used for filtering Rayleigh scattering.
Described upper strata metallic film, lower metal film, side metal film are made by silver, gold or aluminium, and thickness range is 30nm-100nm; Described glass is the glass of thickness 0.5mm～2.0mm.
The method has following characteristics:
(1) sample is as the ducting layer of waveguide, and wherein light field has high energy density with the transmission of oscillating field form, for the favourable exciting light condition that provides is provided Raman;
(2) the Ultra-High Order guided mode that utilizes free-space coupling technology to excite is a kind of slow wave, but the interaction distance of Effective Raise light field and test substance can significantly improve raman spectral signal intensity;
(3) because double-sided metal coats, and the effective refractive index of Ultra-High Order guided mode can go to zero, and this shows that the sample refractive index can be unrestricted, to being very favourable near the research of the biological sample under the state of nature under the aqueous environment.
This waveguide Raman Enhancement Method has high sensitivity from principle, high resolving power and the characteristics such as simple in structure, be applicable to the direct or indirect raman spectroscopy measurement of the biological samples such as blood of human body, urine, cell, also be suitable for combining with existing SERS means, the realization secondary strengthens, and might break through existing detection level and sensitivity.
Description of drawings
Fig. 1 optical waveguide of the present invention strengthens machine-processed Raman principle schematic
Measure in (b) non-waveguide pond in Raman spectrum (a) the waveguide pond of Fig. 2 50mMol D/W and measure
The accompanying drawing illustration
The 1-light source
2-lower metal film
3-upper strata glass
The 4-sample cell
5-lower metal film
6-side metal film
7-lower floor glass
8-side Raman probe
The 9-sample
The 10-Raman diffused light
The 11-Rayleigh scattering.
Embodiment
Below by drawings and Examples the present invention is described in further detail, Fig. 1 is a kind of optical waveguide Raman spectrometer, be provided with upper strata metallic film (2) from top to bottom, upper strata glass (3), sample cell (4), lower metal film (5), lower floor's glass (7), metallic film (2) and lower metal film (5) form the clad of waveguide at the middle and upper levels for they, upper strata glass (3), sample cell (4) forms ducting layer, side metal film (6) is close to above upper strata metallic film (2), upper strata glass (3), sample cell (4), lower metal film (5), lower floor's glass (7), side metal film (6) top is concordant with upper strata metallic film (2) top, side metal film (6) bottom is concordant with lower metal film (5) bottom, laser is as light source (1), behind light source (1) the incident upper strata metallic film (2), through sample cell (4) scattering occurs, side Raman probe (8) receives Raman diffused light (10).
Inject biological sample (9) in the described sample cell (4), chemical solution or material, the large wave band of optical responsivity of sample (9) is determined the thickness of upper strata metallic film (2).
Described sample cell (4) is millimetre-sized cavity, and two ends respectively connect a passage, and sample (9) can be injected in passage two, realizes Real-Time Monitoring.
Described side Raman probe (8) comprises a notch filter sheet that is used for filtering Rayleigh scattering (11).
Described upper strata metallic film (2), lower metal film (5), side metal film (6) are made by silver, gold or aluminium, and thickness range is 30nm-100nm; Described glass (3) is the glass of thickness 0.5mm～2.0mm.
Above said content only is the basic explanation of the present invention under conceiving, and according to any equivalent transformation that technical scheme of the present invention is done, all should belong to protection scope of the present invention.
Claims (10)
1. the method that strengthens of a Raman spectrum, by realizing with lower device: be provided with upper strata metallic film (2) from top to bottom, upper strata glass (3), sample cell (4), lower metal film (5), lower floor's glass (7), metallic film (2) and lower metal film (5) form the clad of waveguide at the middle and upper levels for they, upper strata glass (3), sample cell (4) forms ducting layer, side metal film (6) is close to above upper strata metallic film (2), upper strata glass (3), sample cell (4), lower metal film (5), lower floor's glass (7), side metal film (6) top is concordant with upper strata metallic film (2) top, side metal film (6) bottom is concordant with lower floor's glass (7) bottom, laser is as light source (1), behind light source (1) the incident upper strata metallic film (2), through sample cell (4) scattering occurs, side Raman probe (8) receives Raman diffused light (10).
2. method that Raman spectrum as claimed in claim 1 strengthens, it is characterized in that: according to injecting biological sample (9) in the described sample cell (4), chemical solution or material, the large wave band of optical responsivity of sample (9) is determined the thickness of upper strata metallic film (2).
3. method that Raman spectrum as claimed in claim 1 strengthens, it is characterized in that: described sample cell (4) is millimetre-sized cavity, and two ends respectively connect a passage, sample (9) can be injected in passage two, realizes Real-Time Monitoring.
4. method that Raman spectrum as claimed in claim 1 strengthens, it is characterized in that: described side Raman probe (8) comprises a notch filter sheet that is used for filtering Rayleigh scattering (11).
5. method that Raman spectrum as claimed in claim 1 strengthens is characterized in that: described upper strata metallic film (2), and lower metal film (5), side metal film (6) are made by silver, gold or aluminium, and thickness range is 30nm-100nm; Described upper strata glass (3), lower floor's glass (7) are the glass of thickness 0.5mm～2.0mm.
6. one kind is used for the device that Raman spectrum strengthens: be provided with upper strata metallic film (2) from top to bottom, upper strata glass (3), sample cell (4), lower metal film (5), lower floor's glass (7), metallic film (2) and lower metal film (5) form the clad of waveguide at the middle and upper levels for they, upper strata glass (3), sample cell (4) forms ducting layer, side metal film (6) is close to above upper strata metallic film (2), glass (3), sample cell (4), lower metal film (5), lower floor's glass (7), side metal film (6) top is concordant with upper strata metallic film (2) top, side metal film (6) bottom is concordant with lower floor's glass (7) bottom, laser is as light source (1), behind light source (1) the incident upper strata metallic film (2), through sample cell (4) scattering occurs, side Raman probe (8) receives Raman diffused light (10).
7. device that Raman spectrum as claimed in claim 6 strengthens, it is characterized in that: according to injecting biological sample (9) in the described sample cell (4), chemical solution or material, the large wave band of optical responsivity of sample (9) is determined the thickness of upper strata metallic film (2).
8. device that Raman spectrum as claimed in claim 6 strengthens, it is characterized in that: described sample cell (4) is millimetre-sized cavity, and two ends respectively connect a passage, sample (4) can be injected in passage two, realizes Real-Time Monitoring.
9. device that Raman spectrum as claimed in claim 6 strengthens, it is characterized in that: described side Raman probe (8) comprises a notch filter sheet that is used for filtering Rayleigh scattering (11).
10. device that Raman spectrum as claimed in claim 6 strengthens is characterized in that: described upper strata metallic film (2), and lower metal film (5), side metal film (6) are made by silver, gold or aluminium, and thickness range is 30nm-100nm; Described upper strata glass (3), lower floor's glass (7) are the glass of thickness 0.5mm～2.0mm.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103630973A (en) * | 2013-12-17 | 2014-03-12 | 哈尔滨理工大学 | Production method for liquid-core optical fibre and quartz optical fibre coupling device |
| CN103822911A (en) * | 2013-12-11 | 2014-05-28 | 华东交通大学 | Raman spectrum enhancing device based on optical waveguide oscillating field sensor |
| CN105675536A (en) * | 2016-01-19 | 2016-06-15 | 首都师范大学 | Metal grating surface plasma effect biological-detection chip for THz-TDS system |
| CN107179311A (en) * | 2017-06-27 | 2017-09-19 | 复拓科学仪器（苏州）有限公司 | Waveguide Raman scattering chamber based on couple prism twice |
| CN107764778A (en) * | 2017-08-25 | 2018-03-06 | 复拓科学仪器（苏州）有限公司 | Zero group velocity resonance biological interaction of molecules detection method and detection means |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994024543A1 (en) * | 1993-04-15 | 1994-10-27 | Japan Energy Corporation | Total reflection type sensor for measuring refraction index |
| US6560259B1 (en) * | 2000-05-31 | 2003-05-06 | Applied Optoelectronics, Inc. | Spatially coherent surface-emitting, grating coupled quantum cascade laser with unstable resonance cavity |
| CN1425910A (en) * | 2002-12-16 | 2003-06-25 | 吴世法 | Near field enhanced raman scattering sample pool with exciting and receiving implicit light |
| CN1595121A (en) * | 2004-06-24 | 2005-03-16 | 上海交通大学 | Optical waveguide biochemical sensor and measurement system based on free space coupling |
| US20060068490A1 (en) * | 2004-03-05 | 2006-03-30 | Cha-Mei Tang | Flow-through chemical and biological sensor |
| US20070280309A1 (en) * | 2006-05-23 | 2007-12-06 | Ansheng Liu | Optical waveguide with single sided coplanar contact optical phase modulator |
| CN100401041C (en) * | 2005-06-09 | 2008-07-09 | 上海交通大学 | Light waveguide absorption type gas sensor and measuring system |
| CN101281301A (en) * | 2008-05-22 | 2008-10-08 | 上海交通大学 | Polarization irrelevant crystal electro optic modulator based on two-sided metallic reflection |
| CN101413827A (en) * | 2008-06-19 | 2009-04-22 | 上海交通大学 | Method for detecting laser wavelength by Goos-Hanchen displacement characteristic |
| CN102456761A (en) * | 2010-11-02 | 2012-05-16 | 方靖淮 | Film solar cell |
-
2012
- 2012-11-28 CN CN201210493141.2A patent/CN102967593B/en active Active
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994024543A1 (en) * | 1993-04-15 | 1994-10-27 | Japan Energy Corporation | Total reflection type sensor for measuring refraction index |
| US5565978A (en) * | 1993-04-15 | 1996-10-15 | Japan Energy Corporation | Total-reflection type refractive index sensor |
| US6560259B1 (en) * | 2000-05-31 | 2003-05-06 | Applied Optoelectronics, Inc. | Spatially coherent surface-emitting, grating coupled quantum cascade laser with unstable resonance cavity |
| CN1425910A (en) * | 2002-12-16 | 2003-06-25 | 吴世法 | Near field enhanced raman scattering sample pool with exciting and receiving implicit light |
| US20060068490A1 (en) * | 2004-03-05 | 2006-03-30 | Cha-Mei Tang | Flow-through chemical and biological sensor |
| CN1595121A (en) * | 2004-06-24 | 2005-03-16 | 上海交通大学 | Optical waveguide biochemical sensor and measurement system based on free space coupling |
| CN100401041C (en) * | 2005-06-09 | 2008-07-09 | 上海交通大学 | Light waveguide absorption type gas sensor and measuring system |
| US20070280309A1 (en) * | 2006-05-23 | 2007-12-06 | Ansheng Liu | Optical waveguide with single sided coplanar contact optical phase modulator |
| CN101281301A (en) * | 2008-05-22 | 2008-10-08 | 上海交通大学 | Polarization irrelevant crystal electro optic modulator based on two-sided metallic reflection |
| CN101413827A (en) * | 2008-06-19 | 2009-04-22 | 上海交通大学 | Method for detecting laser wavelength by Goos-Hanchen displacement characteristic |
| CN102456761A (en) * | 2010-11-02 | 2012-05-16 | 方靖淮 | Film solar cell |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103822911A (en) * | 2013-12-11 | 2014-05-28 | 华东交通大学 | Raman spectrum enhancing device based on optical waveguide oscillating field sensor |
| CN103630973A (en) * | 2013-12-17 | 2014-03-12 | 哈尔滨理工大学 | Production method for liquid-core optical fibre and quartz optical fibre coupling device |
| CN103630973B (en) * | 2013-12-17 | 2015-08-05 | 哈尔滨理工大学 | The method for making of liquid-core optical fibre and silica fibre coupling device |
| CN105675536A (en) * | 2016-01-19 | 2016-06-15 | 首都师范大学 | Metal grating surface plasma effect biological-detection chip for THz-TDS system |
| CN105675536B (en) * | 2016-01-19 | 2018-05-04 | 首都师范大学 | Metal grating surface plasma bulk effect biological detection chip for THz-TDS systems |
| CN107179311A (en) * | 2017-06-27 | 2017-09-19 | 复拓科学仪器（苏州）有限公司 | Waveguide Raman scattering chamber based on couple prism twice |
| CN107764778A (en) * | 2017-08-25 | 2018-03-06 | 复拓科学仪器（苏州）有限公司 | Zero group velocity resonance biological interaction of molecules detection method and detection means |
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