CN1586430A - Inducing method for exciting large yellow croaker genogenesis diploid by drumfish sperm - Google Patents
Inducing method for exciting large yellow croaker genogenesis diploid by drumfish sperm Download PDFInfo
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- CN1586430A CN1586430A CNA2004100601480A CN200410060148A CN1586430A CN 1586430 A CN1586430 A CN 1586430A CN A2004100601480 A CNA2004100601480 A CN A2004100601480A CN 200410060148 A CN200410060148 A CN 200410060148A CN 1586430 A CN1586430 A CN 1586430A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The present invention relates to fish cultivation, and is especially method of adopting heterogenic sperm to induce large yellow croaker genogenesis diploid. The method includes the following steps: artificial fertilization to mix the sperm of the the same coordinal and not congeneric male parent and the egg of large yellow croaker; stimulation with sea water; pressurizing the fertilized egg inside a pressure container to target pressure of 38-55 MPa; and lowering the pressure in 1-30 min to complete the pressurized shock inducing. The said process is simple, feasible, safe and reliable, and can ensure the survived individuals are genogenesis diploid.
Description
Technical field
The present invention relates to the breed of a kind of Fish, especially adopt the allos sperm to induce the diplontic method of large yellow croaker gynogenesis.
Background technology
(Pseudosciaena crocea Richardon) is female parent with Carnis Pseudosciaenae, light Nibea albiflora (Nibea Chui, Trewavas) Huo shape Nibea albiflora (Nibea miichthioides Chu Lo ﹠ amp; Wu) or equal different the kind in the inter-genera distant hybridization test that Fish are male parent such as Sciaenops ocellatus (Sciaenopsocellatus Linnaeus), rate of fertilization is higher, but incubation rate is almost nil, the monoploid symptom that the dead embryo tool is fairly obvious, though few partial fertilization ovum is arranged can grow to hatching, but it is individual that the hatching prelarva is deformity, do not have and survive to the individuality of initial feeding.Hybridization embryo typical monoploid symptom shows as:cell division is complete, the spilting of an egg is irregular, cell boundaries is fuzzy, body is short and small, pricardial coelom enlarges, muscle segment is fuzzy, hypopigmentation, blood circulation not entirely, spinal curvature etc.At present about successfully inducing the diplontic method of large yellow croaker gynogenesis also not see formal report.Triploid successfully the inducing of relevant Carnis Pseudosciaenae is detected in:1) preliminary study (Wang Jun, Wang Dexiang, the You Yingzhe of the Carnis Pseudosciaenae triploid induction of Wang Jun etc., Deng, the preliminary study of Carnis Pseudosciaenae triploid induction [J], Xiamen University's journal natural science edition, 2001,40 (4): 927-930); 2) hydrostatic pressing of Lin Qi etc. shock induce the Carnis Pseudosciaenae triploid (Lin Qi, Wu Jianshao, Ceng Zhinan, the hydrostatic pressing shock is induced Carnis Pseudosciaenae triploid [J], Marine Sciences, 2001,25 (9): 6-9) .The abductive approach of other Fish gynogenesis diploid is generally as follows:the fresh and alive sperm of getting Fish of the same race, dilute with spermatozoa diluent, place irradiation under ultraviolet or X ray or the gamma-rays, carry out artificial insemination with fresh mature egg behind the certain hour, then adopt methods such as hydrostatic pressing to induce diploid.Because sperm is subjected to the roentgenization inactivation, only possess the ability that activates the ovum growth and the genetic regulation that does not participate in germ cell, so germ cell is a gynogenetic haploid.The deficiency of these class methods is:1) sperm needs inactivation to handle, and is not easy to execute-in-place; 2) operation of sperm inactivation will contact ultraviolet or X ray or gamma-rays irradiation down, has certain danger; 3) condition of sperm inactivation operation needs secular test to grope, and is not easy to grasp; 4) because the operation of sperm inactivation can not be very thorough, so must be mixed with normal diploid or triploid in the gynogenesis filial generation, bring difficulty for the analysis of experimental result.
Summary of the invention
It is pure that the present invention aims to provide a kind of result, need not the sperm inactivation handles, and rate of fertilization, inductivity and survival rate height are convenient to execute-in-place, safe and reliable, simple Shishou fish sperm excites the diplontic method of large yellow croaker gynogenesis of inducing.The present invention adopts the allos sperm to carry out inductive method for this reason.
Step of the present invention is:
1. adopt the artificial fertilization, the sperm of equal not male parent of the same race in the cross combination is mixed with the ovum of Carnis Pseudosciaenae;
2. after smart ovum being mixed, with sea-water activated;
3. germ cell is put into pressure vessel, exert pressure;
4. stop to exert pressure when reaching target pressurizing force 38～55MPa;
5. inducing of pressure shock finished in blood pressure lowering behind lasting 1～30min.
In step 2) in, the water temperature that activates the used sea water of smart ovum is preferably 18～25 ℃.In step 3), the after fertilization time of exerting pressure is behind after fertilization 1～10min germ cell to be put into pressure vessel, exerts pressure; The time of exerting pressure of after fertilization the best is after fertilization 2.5min.In step 4), stop to exert pressure when preferably reaching target pressurizing force 40～45MPa, inducing of pressure shock finished in blood pressure lowering behind lasting 10～15min.
The invention has the advantages that:
1) need not carry out the ray inactivation to the allos sperm and handle, simple possible is convenient to execute-in-place, and is safe and reliable;
2), be convenient to grasp to empirical less demanding;
3) distant hybridization produces monoploid, and haploid lethal effect has been rejected and successfully do not carried out the inductive individuality of diploid, has guaranteed that the survival individuality is gynogenesis diploid.
The specific embodiment
Embodiment 1
The sperm of light Nibea albiflora is mixed with the ovum of Carnis Pseudosciaenae, adopt the dry method fertilization, water temperature is 21～24 ℃, after fertilization 2.5min puts into pressure vessel with germ cell and exerts pressure, stop to exert pressure when target pressurizing force reaches 42～44MPa, inducing of pressure shock promptly finished in blood pressure lowering behind lasting 10～15min.Experimental result shows, rate of fertilization 〉=80%, incubation rate 〉=50%, period of embryo diploid inductivity 〉=88%.
Embodiment 2
The sperm of Sciaenops ocellatus is mixed with the ovum of Carnis Pseudosciaenae, adopt the dry method fertilization, water temperature is 20～23 ℃, after fertilization 1min puts into pressure vessel with germ cell and exerts pressure, stop to exert pressure when target pressurizing force reaches 40～42MPa, inducing of pressure shock promptly finished in blood pressure lowering behind lasting 2～2.5min.Experimental result shows, rate of fertilization 〉=82%, incubation rate 〉=84.8%, period of embryo diploid inductivity 〉=93.75%.
Embodiment 3
Similar to embodiment 1, the water temperature that its difference is to activate used sea water is 18～20 ℃, and after fertilization 10min puts into pressure vessel with germ cell and exerts pressure, and stops when target pressurizing force reaches 38～40MPa exerting pressure, and continues blood pressure lowering behind 1～4min, promptly finishes inducing of pressure shock.Experimental result shows, rate of fertilization 〉=80%, incubation rate 〉=81%, period of embryo diploid inductivity 〉=75%.
Embodiment 4
Similar to embodiment 2, the water temperature that its difference is to activate used sea water is 23～25 ℃, and after fertilization 8min puts into pressure vessel with germ cell and exerts pressure, and stops when target pressurizing force reaches 50～55MPa exerting pressure, inducing of pressure shock promptly finished in blood pressure lowering behind lasting 25～30min.Experimental result shows, rate of fertilization 〉=78%, incubation rate 〉=45%, period of embryo diploid inductivity 〉=98%.
Embodiment 5
Similar to embodiment 1, the water temperature that its difference is to activate used sea water is 20～22 ℃, and after fertilization 4min puts into pressure vessel with germ cell and exerts pressure, and stops when target pressurizing force reaches 45～50MPa exerting pressure, inducing of pressure shock promptly finished in blood pressure lowering behind lasting 15～20min.Experimental result shows, rate of fertilization 〉=81.1%, incubation rate 〉=65%, period of embryo diploid inductivity 〉=98%.
Embodiment 6
Similar to embodiment 2, the water temperature that its difference is to activate used sea water is 21～23 ℃, and after fertilization 6min puts into pressure vessel with germ cell and exerts pressure, and stops when target pressurizing force reaches 40～45MPa exerting pressure, and continues blood pressure lowering behind 5～10min, promptly finishes inducing of pressure shock.Experimental result shows, rate of fertilization 〉=78.8%, incubation rate 〉=77%, period of embryo diploid inductivity 〉=75%.
Claims (6)
1, the Shishou fish sperm excites the diplontic abductive approach of large yellow croaker gynogenesis, it is characterized in that the steps include:
1) adopts the artificial fertilization, the sperm of equal not male parent of the same race in the cross combination is mixed with the ovum of Carnis Pseudosciaenae;
2) with after the smart ovum mixing, with sea-water activated:
3) germ cell is put into pressure vessel, exert pressure;
Stop to exert pressure when 4) reaching target pressurizing force 38～55MPa;
5) inducing of pressure shock finished in blood pressure lowering behind lasting 1～30min.
2, Shishou fish sperm as claimed in claim 1 excites the diplontic abductive approach of large yellow croaker gynogenesis, it is characterized in that in step 2) in, activating the used sea water water temperature of germ cell is 18～25 ℃.
3, Shishou fish sperm as claimed in claim 1 excites the diplontic abductive approach of large yellow croaker gynogenesis, it is characterized in that in step 3), and the after fertilization time of exerting pressure is behind after fertilization 1～10min germ cell to be put into pressure vessel, exerts pressure.
4, Shishou fish sperm as claimed in claim 3 excites the diplontic abductive approach of large yellow croaker gynogenesis, it is characterized in that in step 3), and the after fertilization time of exerting pressure is after fertilization 2.5min.
5, Shishou fish sperm as claimed in claim 1 excites the diplontic abductive approach of large yellow croaker gynogenesis, it is characterized in that in step 4), stops to exert pressure when reaching target pressurizing force 40～45MPa.
6, Shishou fish sperm as claimed in claim 1 excites the diplontic abductive approach of large yellow croaker gynogenesis, it is characterized in that in step 5), and inducing of pressure shock finished in blood pressure lowering behind lasting 10～15min.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2004100601480A CN100435751C (en) | 2004-06-28 | 2004-06-28 | Inducing method for exciting large yellow croaker genogenesis diploid by drumfish sperm |
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| CNB2004100601480A CN100435751C (en) | 2004-06-28 | 2004-06-28 | Inducing method for exciting large yellow croaker genogenesis diploid by drumfish sperm |
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| CN1586430A true CN1586430A (en) | 2005-03-02 |
| CN100435751C CN100435751C (en) | 2008-11-26 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100531684C (en) * | 2005-09-07 | 2009-08-26 | 集美大学 | Method for inducing gynogenetic diploid of Pseudosciaena crocea |
| CN106172117A (en) * | 2016-07-14 | 2016-12-07 | 浙江省海洋水产研究所 | Carnis Pseudosciaenae raun and Carnis Pseudosciaenae milter cross breeding method |
| CN111264423A (en) * | 2020-03-27 | 2020-06-12 | 浙江省海洋水产养殖研究所 | Seedling raising method for acanthocephalus spinosus |
-
2004
- 2004-06-28 CN CNB2004100601480A patent/CN100435751C/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100531684C (en) * | 2005-09-07 | 2009-08-26 | 集美大学 | Method for inducing gynogenetic diploid of Pseudosciaena crocea |
| CN106172117A (en) * | 2016-07-14 | 2016-12-07 | 浙江省海洋水产研究所 | Carnis Pseudosciaenae raun and Carnis Pseudosciaenae milter cross breeding method |
| CN106172117B (en) * | 2016-07-14 | 2019-08-06 | 浙江省海洋水产研究所 | Little yellow croaker raun and Larimichthys crocea milter cross breeding method |
| CN111264423A (en) * | 2020-03-27 | 2020-06-12 | 浙江省海洋水产养殖研究所 | Seedling raising method for acanthocephalus spinosus |
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| CN100435751C (en) | 2008-11-26 |
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Granted publication date: 20081126 Termination date: 20110628 |