CN1559228A - Synergetic type microbial synergistic pesticide used for preventing and controlling plant diseases, and its prepn. method - Google Patents
Synergetic type microbial synergistic pesticide used for preventing and controlling plant diseases, and its prepn. method Download PDFInfo
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Abstract
A synergistic microbial agricultural chemical for preventing and controlling plant diseases is prepared through inoculating Bs-916 bacterial strains in culture medium for activating it, culturing the activated bacterial strains in the culture containing Mixianan with different concentrations to obtain resistant bacterial strains, culturing them in YPG culture liquid, and mixing with Mixianan.
Description
Technical field
The present invention relates to a kind of Recompounded pesticide of controlling plant diseases, the Recompounded pesticide of especially a kind of microorganism and chemical pesticide the invention still further relates to the preparation method of this Recompounded pesticide simultaneously, belongs to agricultural technology field.
Background technology
As everyone knows, the disease on paddy rice, fruit tree and the vegetables is various, and harm is serious.Wherein, the generation of some disease, development have had a strong impact on the fine quality of paddy rice, fruit tree and vegetables.According to the knowledge of the applicant, the control of crop pest mainly relies on chemical pesticide at present.Though a large amount of uses of chemical pesticide are guaranteeing that higher yield of crops, control disease have played effective effect aspect popular, have also brought serious day by day negative effect to human existence simultaneously.
On the one hand, chemical pesticide is when preventing and treating disease, killed the natural enemy of these diseases inevitably, thereby make the biocenological effect of control evil naturally in field seriously be suppressed, pathogen is more and more higher to the pesticide resistance level of agricultural chemicals, the pest control effect decline of agricultural chemicals finally causes producing dosage and spraying times constantly increases, and has polluted the serious consequence of farmland ecological environment.On the other hand, a large amount of uses of agricultural chemicals have had a strong impact on the natural quality of agricultural product, and especially after China joined WTO, the residual formation of agricultural chemicals in agricultural product seriously hinders the green barrier of agricultural products in China outlet.In the face of new demand situation and the requirement of agricultural product, be necessary to study new disease control thinking, foundation is core with the natural fine quality that ensures agricultural product, the development environment compatibility is good, low-residual, to the environment friendly agricultural of people and animals' nonhazardous, the technical support that provides of the sustainable development of China's agricultural is provided.
For example, rice green smut (pathogen is a sac fungi: Ustilaginoidea virens), the pears black spot (pathogen is an imperfect fungus: Alternaria kikuchiana Tanaka), the solanaceous vegetable fusarium wilt (pathogen is an imperfect fungus: Fusarium) and leaf mold (pathogen is imperfect fungus Fulvia fulva) be disease, generally taking place all over the world, be the important disease on grain, the fruits and vegetables.Wherein, the rice green smut incidence of disease can be up to 44.1%, and production loss reaches 26.9%, become one of important disease of Rice Production.Especially rice false smut can produce the toxin that people and animals are harmful to, and mixes the rice false smut in sick paddy, to people's directly harm of health formation, becomes the outstanding problem of the nuisanceless production of paddy rice, has had a strong impact on the high-quality and safety of rice.
The pears black spot is interior main disease breeding time of pears, each pears producing region that spreads all over the country, and this pathogen all can be caused harm in the pears phase of bearing fruit, and influenced output.Secondly, the phase of bearing fruit of pears is long, and frequently medication of the control of this disease in breeding time, and dosage is big, so residue of pesticide seriously influence the quality of pear has formed potential harm to people's health.
Fusarium wilt and cladosporium leaf and fruit mould of tomato are the main diseases of solanaceous vegetable, and the frequency of utilization and the usage amount of agricultural chemicals is maximum in all crops on the vegetables at present.Wilt causes plant to be wilted, and is last dead, is destructive disease; Leaf mold all has generation in China various places, and it is the heaviest especially to be injured with protection ground tomato, underproduction 20%-30% when serious.Controls such as soil treatment, fumicants are mainly adopted in the fusarium wilt of solanaceous vegetable and the control of cladosporium leaf and fruit mould of tomato at present, because high-frequency use agricultural chemicals has polluted farm environment, and the residual quality that influences vegetables of while agricultural chemicals.
Along with the raising of rapid economy development and living standards of the people, people are more and more higher to the requirement of agricultural product quality and output.When as far as possible reducing the production loss that causes by damage by disease and insect, reduce the consumption of chemical pesticide and residual, keep the natural quality of agricultural product, become people's common recognition.Therefore, it is very important and urgent to develop new control medicament, especially biopesticide.
Heartening is, has the bacterial strain that the pathogen of multiple fungal disease is had antagonism in the human bacteria flora of finding to have a kind of bacillus subtilis bacteria microorganism.All carried out with bacillus subtilis both at home and abroad at present and prevented and treated the research of crop pest with antagonism.The main fusarium wilt (Mahaffee of external report to cotton, tomato, W.Rosales A.M.Effects of seedfactors on Spermoshpere and rhizoshpere colonization of cotton byBacillus subtilis GB03, Phtopathology83,1120-1125), the research report (Kong Jian etc., 1992 " research of bacillus subtilis Bs-903 bacterial strain antibacterial material " " microorganism journal " 32 (6)) that domestic researcher has delivered the bacillus subtilis antibacterial material and its antibacterial protein is separated.But understand according to the applicant, not seeing so far has the microorganism Recompounded pesticide that the various crop disease that comprises above-mentioned four kinds of diseases is all had good control efficiency with behind bacillus subtilis and certain agriculture chemical compounding.
Summary of the invention
The objective of the invention is to: go up a large amount of chemical pesticide control corps diseases that use in the face of producing at present, the severe contamination farmland ecological environment, cause residue of pesticide to exceed standard, influence the situation of quality of agricultural product, by studying the composite of bacillus subtilis and chemical pesticide, introduce microbial bacterial agent, a kind of Synergistic type microorganism Recompounded pesticide that various common crop pests is all had good control efficiency is proposed, provide the preparation method of this Recompounded pesticide simultaneously, thereby realize the reasonable distribution of micropopulation in the handle management ecotope, worsen the living environment of pathogen, form stable in conjunction with cultivation management and chemical control measure, balance, bio-diversity field ecosystem, effectively, control the popular of crop pest more enduringly, effectively reduce the usage amount of chemical pesticide simultaneously, improve farmland ecological environment, reduce residue of pesticide, be beneficial to keep the fine quality of agricultural product, ensure agricultural product security.
The applicant gropes and contrast experiment repeatedly through constantly exploring, and has formed the technical scheme of Recompounded pesticide of the present invention, and its main points are: bacillus subtilis Bs-916 bacterial strain (hereinafter to be referred as the Bs-916 bacterial strain) is transplanted on bacteria culture media activated; Preparation contains the bacteria culture media that prochloraz concentration increases progressively, the immigration of Bs-916 bacterial strain after the activation is contained in the bacteria culture media of low concentration prochloraz, after treating that growth is good, move into again and contain in the bacteria culture media that prochloraz concentration increases progressively, and the like, up to obtaining to have drug-fast Bs-916 resistant strain; The Bs-916 bacterial strain of anti-prochloraz is moved into inoculum cultivation acquisition culture fluid microbial inoculum; The microbial inoculum that obtains is prepared with prochloraz, can be obtained the present invention by composite dose of the formulated Synergistic type microorganism of the Bs-916 microbial inoculum of anti-prochloraz and prochloraz.
Need to prove, above-mentioned bacillus subtilis Bs-916 bacterial classification is in Chinese representative microbial preservation center (CGMCC) preservation in the Pekinese in 2002 on September 30,, deposit number is: CGMCCNo.0808, Latin formal name used at school are bacillus subtilis Bs-916 (this paper is called for short Bs-916).Its biological character is recited as: colonial morphology: unicellular formation bacterium colony circle, and smooth all around, YPGA (yeast extract 5.0g, tryptone 5.0g; Glucose 5.0g, agar 17.0g; Water 1000ml) the bacterium colony 3-5mm of growth 24h on the culture medium flat plate, cream-coloured, there is gauffer on the surface.Diagnostic character: the Gram reaction negative, strict good gas, 2%NaCl can not grow, and lactulose, L-arabinose, L-lactic acid, melezitose and D-malic acid utilization reaction are all negative, and glycerine, sucrose, maltose and cellobiose utilization reaction are all positive.
More specifically, Synergistic type microorganism Recompounded pesticide employing following steps of the present invention preparation:
1, activation: bacillus subtilis Bs-916 bacterial strain transplanted on bacteria culture media activate.
2, pesticide resistance is induced: bacteria culture media is mixed with band prochloraz flat board, and wherein the concentration of prochloraz increases progressively, and for example is 5ug/ml, 10ug/ml, and 20ug/ml....... is until 1000ug/ml.Bs-916 bacterial strain after the activation moved into contain the low concentration prochloraz, for example in the flat board of 5ug/ml, treat that growth is good after, move into again in the flat board of the 10ug/ml that concentration increases progressively, and the like, up to the Bs-916 resistant strain that obtains on 1000ug/ml, to grow.
3, obtain culture fluid: after will resisting the Bs-916 bacterial strain activation of prochloraz, move into inoculum, obtained the culture fluid microbial inoculum in shaken cultivation 22-55 hour in 15-38 ℃, bacterial content is 2-5 * 10
10Cfu/ml.
4, composite patent medicine: will make the microbial inoculum and preparation in proportion that obtain, and obtain composite dose of Synergistic type microorganism of the present invention.
Understand easily, above prochloraz is a generalized concept, promptly comprises various prochloraz salts (for example Chang Yong prochloraz manganese salt etc.).
The beneficial effect of microorganism Recompounded pesticide of the present invention is as follows:
1, according to the microecology principle, by introducing microbial bacterial agent, the reasonable distribution of microbiologic population in the handle management ecotope, worsen the living environment of pathogen, in conjunction with cultivation management, agricultural and chemical control measure, form stable, balance, a bio-diversity field ecosystem, thereby control the popular of various crop disease effectively, more enduringly.
2, microbial pesticide has the not available advantage of chemical agent, it is controlled the disease mechanism of action and is mainly: the 1. competition effect of dominant population---bacillus subtilis Bs-916 has the advantages that colonization ability is strong on plant, breeding is fast, can infect the site with the pathogen effective competition, reduce disease morbidity risk rate (referring to table 1).2. the plain antibiosis of antagonism---bacillus subtilis Bs-916 can secrete generation antagonism element, its can control effectively disease growth, infect and spread (referring to table 2).3. the induction of resistance effect of antagonism bacterium---bacillus subtilis Bs-916 can induce a large amount of generations enzyme relevant with disease resistance in the plant behind foliage-spray, resist the ability that pathogen is invaded, spreads thereby strengthened plant.
3, composite dose synergistic effect, after the chemical pesticide prochloraz of bacillus subtilis Bs-916 and high-efficiency low-toxicity is composite, the effect that has mutual supplement with each other's advantages between component, can bring into play prochloraz efficiently, bacteriostasis fast, can bring into play antagonism bacterium Bs-916 again and grow, breed in a large number the adjustment function that back microbiologic population rationally distributes in the field surely, thereby reach the purpose of sustainable Control disease, reduced the usage amount of chemical pesticide simultaneously.
4, carry out composite with arbitrary proportion fermentation of bacillus subtilis liquid and prochloraz, as long as choose appropriate compound proportion, the application of agricultural chemicals of the present invention is reached to bring into play the optimum medicine efficacy effect, can reduce the usage amount and the access times of chemical pesticide again, keep the purpose of agricultural product natural quality as far as possible.
Theory and practice can prove; use Recompounded pesticide spraying of the present invention in the field, irritate root etc.; almost multiple common crop pest all had good control efficiency; minimizing residue of pesticide, the ecotope that protects the fields had positive effect; help keeping the fine quality of agricultural product, ensure agricultural product security.
Embodiment
Example one: with bacillus subtilis (Bs-916) and prochloraz composite after, spraying control rice green smut
According to the composite Synergistic type microorganism Recompounded pesticide of the present invention of following steps: bacillus subtilis Bs-916 bacterial strain transplanted on the YPGA medium activate; Medium is mixed with band prochloraz flat board, and wherein the concentration of prochloraz is 5ug/ml, 10ug/ml, and 20ug/ml....... is until 1000ug/ml.Bs-916 bacterial strain after the activation is moved in the flat board of 5ug/ml, treat that growth is good after, move into again in the flat board of 10ug/ml, and the like, up to the Bs-916 resistant strain that obtains on 1000ug/ml, to grow; After the Bs-916 bacterial strain activation with anti-prochloraz, move into the YPG culture fluid, 28 ℃ of shaken cultivation obtained the culture fluid microbial inoculum in 48 hours, and bacterial content is about 2.5 * 10
10Cfu/ml; Microbial inoculum that system is obtained and prochloraz are mixed with composite dose of Synergistic type microorganism of the present invention by the part by weight of 2: 3 (40%: 60%).
With the Recompounded pesticide of present embodiment spraying control rice green smut, find that it has a predominant suppressing effect to the rice green smut bacterium is indoor; Field test results shows (referring to table 1, annotate: prochloraz manganese salt is a prochloraz), and the field control effect that this composite dose result of the test in Anhui is false smut is 73.14%.Result of the test in Jiangxi shows that during every mu of use 60g, control efficiency is 94.84%.Its control efficiency all is higher than the component medicament, can effectively reduce the usage amount of chemical agent, is beneficial to the fine quality that keeps paddy rice, can reduce the residual quantity of agricultural chemicals.
Example two: with bacillus subtilis (Bs-916) and prochloraz composite after, sprinkle and water and irritate root and prevent and treat tomato wilt
By and the composite Synergistic type microorganism Recompounded pesticide of the present invention of embodiment one similar step, wherein move into the YPG culture fluid after, shaken cultivation acquisition in 50 hours culture fluid microbial inoculum in 18 ℃, bacterial content is slightly larger than 2 * 10
10Cfu/ml; Microbial inoculum that system is obtained and prochloraz are mixed with composite dose of Synergistic type microorganism of the present invention (ProductName is pacified for a long time) by the part by weight of 1: 1 (50%: 50%), 1: 3 (25%: 75%).
The Recompounded pesticide of present embodiment sprinkled water and irritate root and prevent and treat tomato wilt, find that it has good inhibition effect to the Solanaceae wilt.Field test results shows that its control efficiency has good control efficiency (table 2) more than 90%.
Example three: with bacillus subtilis (Bs-916) and prochloraz composite after, spraying prevents and treats cladosporium leaf and fruit mould of tomato
By and the composite Synergistic type microorganism Recompounded pesticide of the present invention of embodiment one similar step, wherein move into the YPG culture fluid after, shaken cultivation acquisition in 45 hours culture fluid microbial inoculum in 32 ℃, bacterial content is about 4 * 10
10Cfu/ml; Microbial inoculum that system is obtained and prochloraz are mixed with composite dose of Synergistic type microorganism of the present invention by the part by weight of 3: 1 (75%: 27%).
The Recompounded pesticide spraying of present embodiment is prevented and treated cladosporium leaf and fruit mould of tomato, and field test results shows that its control efficiency has good control effect more than 80%.Be higher than similar chemical agent (table 3).
Example four: with bacillus subtilis and prochloraz composite after, the pears alternaria is had good inhibitory action.
By and the composite Synergistic type microorganism Recompounded pesticide of the present invention of embodiment one similar step, wherein move into the YPG culture fluid after, shaken cultivation acquisition in 48 hours culture fluid microbial inoculum in 28 ℃, bacterial content is about 4 * 10
10Cfu/ml; Microbial inoculum that system is obtained and prochloraz are mixed with composite dose of Synergistic type microorganism of the present invention by the part by weight of 3: 1 (75%: 27%), 2: 1 (66%: 33%), 1: 1 (50%: 50%), 1: 2 (33%: 66%) 1: 3 (25%: 75%).
After the Recompounded pesticide of present embodiment is used to prevent and treat the pears alternaria, indoor measurement its inhibitory action (table 4) to the pears alternaria, the result shows that this composite dose of bacteriostasis to the pears alternaria obviously improves.Its bacteriostasis all is higher than the component medicament.
Bacteria culture media also can be used LB, NA, KMB etc. except YPGA, inoculum also can be used liquid LB, NA, KMB etc. except YPG.The bacterial content of culture fluid microbial inoculum is greater than 2 * 10
10Cfu/ml is generally 2-5 * 10
10Cfu/ml.In theory, the available weight percentage ranges of above-mentioned preparation is microbial inoculum content 1%-99%, prochloraz 1%-99%; Practicable weight percentage ranges is microbial inoculum content 10%-90%, prochloraz 10%-90%; And usually preferably weight percentage ranges be microbial inoculum content 20%-80%, prochloraz 20%-80%.
In sum, after bacillus subtilis biological and ecological methods to prevent plant disease, pests, and erosion Bs-916 bacterial strain and prochloraz are composite, the various plants pathogen all there is good inhibition effect, disease is also had the better prevention effect.By relevant synergy analysis, the result shows, synergistic effect is remarkable, can effectively control the multiple kinds of crops disease after biocontrol microorganisms Bs-916 and prochloraz are composite, reduce the usage amount of chemical pesticide simultaneously, reduced the selection pressure of chemical pesticide and the risk that pesticide resistance takes place, can reduce residue of pesticide, help ensureing the agricultural product fine quality, similar medicament on control efficiency is better than producing.
Table 1 2.5 * 10
10The bright peace of living spores/ml bacillus subtilis manganese salt WP control rice green smut field test results
| Handle (g/667m 2) | Repeat | The clump number | Investigate total spike number | Investigate 10 grain number per spikes | The total grain of investigation number | The infected seed number | Infected seed rate (%) | Control efficiency (%) | Significance | ||
| ??0.05 | ??0.01 | ||||||||||
| ???2.5 ???×10 10Living spores/ml bacillus subtilis prochloraz-manganese chloride complex WP | ? ? ? ? ? ? ? ??60 | ? ? ? ????1 ? ????2 ? ????3 ? ????4 ? ????x ? | ? ? ? ??100 ? ??100 ? ??100 ? ??100 ? ??100 | ? ? ??1020 ? ??1260 ? ??1010 ? ??980 ? ??1067. ? ??5 | ? ? ??1267 ? ??1185 ? ??1273 ? ??1294 ? ??1254.7 ? ??5 | ??12923 ??4 ??14931 ? ??0 ? ??12857 ? ??3 ? ??12681 ? ??2 ??13348 ??2 | ? ? ? ????48 ? ????75 ? ????51 ? ????33 ? ????51.75 ? | ? ? ? ??0.037 ? ??0.050 ? ??0.040 ? ??0.026 ? ??0.038 ? | ? ? ? ??94.61 ? ??93.06 ? ??95.10 ? ??96.59 ? ??94.84 ? | ? ? ? ? ? ? ? ??a | ? ? ? ? ? ? ? ??A |
| ? ? ? ? ? ? ? ??45 | ? ? ? ????1 ? ????2 ? ????3 ? ????4 ? ????x ? | ? ? ? ??100 ? ??100 ? ??100 ? ??100 ? ??100 ? | ? ? ??1250 ? ??1040 ? ??1240 ? ??1190 ? ??1180. ? ??0 | ? ? ??1098 ? ??1235 ? ??1157 ? ??1219 ? ??1177.2 ? ??5 | ??13725 ??0 ??12844 ? ??0 ? ??14346 ? ??8 ? ??14506 ? ??1 ??13855 ??4 | ? ? ? ????79 ? ????94 ? ????96 ? ????109 ? ????94.50 ? | ? ? ? ??0.058 ? ??0.073 ? ??0.067 ? ??0.075 ? ??0.068 ? | ? ? ? ??91.64 ? ??89.87 ? ??91.74 ? ??90.15 ? ??90.85 ? | ? ? ? ? ? ? ? ??b | ? ? ? ? ? ? ? ??B | |
| ? ? ? ? ? ? ? ??30 | ? ? ? ????1 ? ????2 ? ????3 ? ????4 ? ????x ? | ? ? ? ??100 ? ??100 ? ??100 ? ??100 ? ??100 ? | ? ? ??1220 ? ??1110 ? ??1130 ? ??1180 ? ??1160. ? ??0 | ? ? ??1135 ? ??1228 ? ??1196 ? ??1175 ? ??1183.5 ? ??0 | ??13847 ??0 ??13630 ? ??8 ? ??13514 ? ??8 ? ??13865 ? ??0 ??13714 ??4 | ? ? ????185 ? ????197 ? ????188 ? ????196 ? ????191.5 ? ????0 ? | ? ? ? ??0.134 ? ??0.145 ? ??0.139 ? ??0.141 ? ??0.140 ? | ? ? ? ??80.61 ? ??80.01 ? ??82.83 ? ??81.45 ? ??81.22 ? | ? ? ? ? ? ? ? ??c | ? ? ? ? ? ? ? ??C | |
| 50% prochloraz-manganese chloride complex WP 30 | ? ? ? ??1 ? ??2 ? ??3 ? ??4 ? ??x ? | ? ? ? ??100 ? ??100 ? ??100 ? ??100 ? ??100 | ? ? ????1160 ? ????1070 ? ????1210 ? ????1050 ? ????1122. ? ????5 ? | ? ? ????1249 ? ????1284 ? ????1164 ? ????1253 ? ????1237.5 ? ????0 ? | ??14488 ??4 ??13738 ? ??8 ? ??14084 ? ??4 ? ??13156 ? ??5 ??13867 ??0 | ? ? ????104 ? ????97 ? ????101 ? ????118 ? ????105.0 ? ????0 | ? ? ? ???0.072 ? ???0.071 ? ???0.072 ? ???0.090 ? ???0.076 | ? ? ? ???89.58 ? ???90.23 ? ???91.15 ? ???88.23 ? ???89.80 | ? ? ? ? ? ? ? ????b | ? ? ? ? ? ? ? ????B |
| ? ? ? ? ????5×10 10Living spores/ml bacillus subtilis WP 60 | ? ? ? ??1 ? ??2 ? ??3 ? ??4 ? ??x ? | ? ? ? ??100 ? ??100 ? ??100 ? ??100 ? ??100 ? | ? ? ????1080 ? ????1060 ? ????1170 ? ????1230 ? ????1135. ? ????0 ? | ? ? ????1331 ? ????1315 ? ????1241 ? ????1116 ? ????1250.7 ? | ??14374 ??8 ??13939 ? ??0 ? ??14519 ? ??7 ? ??13726 ? ??8 ? ??14140 ??0 | ? ? ? ????82 ? ????91 ? ????84 ? ????106 ? ????90.75 | ? ? ? ???0.057 ? ???0.065 ? ???0.058 ? ???0.077 ? ???0.064 | ? ? ? ???91.73 ? ???90.97 ? ???92.85 ? ???89.87 ? ???90.36 | ? ? ? ? ? ? ? ????b | ? ? ? ? ? ? ? ????B |
| ? ? ? ? ? ? ? ????CK | ? ? ? ??1 ? ??2 ? ??3 ? ??4 ? ??x ? | ? ? ? ??100 ? ??100 ? ??100 ? ??100 ? ??100 ? | ? ? ????1140 ? ????1120 ? ????1090 ? ????1150 ? ????1125. ? ????0 ? | ? ? ????1239 ? ????1224 ? ????1276 ? ????1181 ? ????1230.0 ? ????0 | ??14124 ??6 ??13708 ? ??8 ? ??13908 ? ??4 ? ??13581 ? ??5 ??13837 ??5 | ? ? ????973 ? ????991 ? ????1127 ? ????1035 ? ????1031. ? ????5 | ? ? ? ???0.690 ? ???0.720 ? ???0.810 ? ???0.760 ? ???0.745 ? | ? ? ? ???/ ? ???/ ? ???/ ? ???/ ? ???/ ? | ||
| Standard error | ??????0.52 | |||||||||
Table 2: antagonistic bacterium, composite dose of control efficiency of microbial pesticide to the solanaceous vegetable fusarium wilt
Handle and handled back 70 days in back 50 days
Average disease refers to that the average disease of preventive effect (%) refers to preventive effect (%)
2 * 10
10Living spores/ml 0 100.00 0 100.00
The bacillus subtilis miaow
Bright amine manganese salt WP 1: 1
2 * 10
10Living spores/ml 0 100.00 1 95.45
Bacillus subtilis 25%
Prochloraz-manganese chloride complex WP 1: 3
Sporgon (low) 0 100.00 0 100.00
Blank 17 22
Table 3 antagonistic bacterium, composite dose of control efficiency of microbial pesticide to the solanaceous vegetable leaf mold
Test medicine makes 28 days control efficiency of preceding 14 days control efficiency of medication
The concentration state of an illness state of an illness state of an illness increases control and imitates state of an illness state of an illness growth control
(doubly) index index percent (%) is (%) index percent (%) effect really
(％)
4 * 10
10Living spores/ml withered grass 1,000 3.40 4.52 32.94 82.59a 4.82 41.76 87.07a
Bacillus prochloraz-manganese chloride complex 500 4.15 5.02 20.96 84.16a 5.40 30.12 88.13a
WP
Metalaxyl-mn-zn 1,000 3.52 8.87 152.00 67.00b 9.78 177.84 74.67b
Carbendazim 700 3.34 9.87 195.51 61.30b 10.23 206.29 72.08b
Contrast clear water 3.51 26.8 663.53 38.5 996.87
Table 4: to the inhibition effect of pears alternaria
| Proportioning | Extension rate | ????Bs-916(10 5cfu/ml) | 50% prochloraz (ppm) | Bacteriostasis rate (%) |
| ????3∶1* | ????1000 | ????1 | ????0.2 | ????83.3 |
| ????2000 | ????0.5 | ????0.1 | ????78.3 | |
| ????2500 | ????0.4 | ????0.08 | ????86.7 | |
| ????5000 | ????0.2 | ????0.04 | ????70.0 | |
| ????10000 | ????0.1 | ????0.02 | ????60.0 | |
| ????2∶1 | ????1000 | ????1 | ????0.2 | ????99.0 |
| ????2000 | ????0.5 | ????0.1 | ????92.3 | |
| ????2500 | ????0.4 | ????0.08 | ????81.6 | |
| ????5000 | ????0.2 | ????0.04 | ????73.3 | |
| ????10000 | ????0.1 | ????0.02 | ????45.0 | |
| ????1∶1 | ????1000 | ????1 | ????0.2 | ????100.0 |
| ????2000 | ????0.5 | ????0.1 | ????92.0 | |
| ????2500 | ????0.4 | ????0.08 | ????88.3 | |
| ????5000 | ????0.2 | ????0.04 | ????83.3 | |
| ????10000 | ????0.1 | ????0.02 | ????53.3 | |
| ????1∶2 | ????1000 | ????1 | ????0.2 | ????100.0 |
| ????2000 | ????0.5 | ????0.1 | ????96.0 | |
| ????2500 | ????0.4 | ????0.08 | ????85.0 | |
| ????5000 | ????0.2 | ????0.04 | ????75.0 | |
| ????10000 | ????0.1 | ????0.02 | ????58.3 | |
| ????1∶3 | ????1000 | ????1 | ????0.2 | ????98.3 |
| ????2000 | ????0.5 | ????0.1 | ????94 | |
| ????2500 | ????0.4 | ????0.08 | ????90.7 | |
| ????5000 | ????0.2 | ????0.04 | ????60.0 | |
| ????10000 | ????0.1 | ????0.02 | ????53.3 | |
| 50% prochloraz | ????0.1 | ????95.0 | ||
| ????0.08 | ????88.3 | |||
| ????0.04 | ????70.0 | |||
| ????0.02 | ????43.3 | |||
| Bacillus subtilis | ????1 | ????70.0 | ||
| ????0.5 | ????50.0 | |||
| ????0.4 | ????23.3 | |||
| ????0.2 | ????15.0 | |||
| ????CK | ????0.0 |
(the * ratio is 2.5 * 10
10Living spores/ml bacillus subtilis: 25% prochloraz-manganese chloride complex)
Claims (9)
1. Synergistic type microorganism Recompounded pesticide that is used for controlling plant diseases is characterized in that: formulated by bacillus subtilis Bs-916 (the Latin formal name used at school bacillus subtilis Bs-916) microbial inoculum of anti-prochloraz and prochloraz or prochloraz salt.
2. according to the described Synergistic type microorganism Recompounded pesticide that is used for controlling plant diseases of claim 1, it is characterized in that: the bacterial content of described anti-prochloraz Bs-916 microbial inoculum is greater than 2 * 10
10Cfu/ml.
3. according to the described Synergistic type microorganism Recompounded pesticide that is used for controlling plant diseases of claim 2, it is characterized in that: the percentage by weight of described anti-prochloraz Bs-916 microbial inoculum and prochloraz preparation is microbial inoculum 10%-90%, prochloraz 10%-90%.
4. according to the described Synergistic type microorganism Recompounded pesticide that is used for controlling plant diseases of claim 2, it is characterized in that: the percentage by weight of described anti-prochloraz Bs-916 microbial inoculum and prochloraz preparation is microbial inoculum 20%-80%, prochloraz 20%-80%.
5. a Synergistic type microorganism Recompounded pesticide preparation method who is used for controlling plant diseases the steps include:
1), activation---bacillus subtilis Bs-916 bacterial strain transplanted on bacteria culture media activate;
2), pesticide resistance is induced---and preparation contains the bacteria culture media that prochloraz concentration increases progressively, the immigration of Bs-916 bacterial strain after the activation is contained in the bacteria culture media of low concentration prochloraz, after treating that growth is good, move into again and contain in the bacteria culture media that prochloraz concentration increases progressively, and the like, up to obtaining to have drug-fast Bs-916 resistant strain;
3), obtain culture fluid---will resist the Bs-916 bacterial strain of prochloraz to move into inoculum cultivation acquisition culture fluid microbial inoculum;
4), composite dose preparation---the microbial inoculum that obtains and prochloraz or prochloraz salt are mixed with composite dose of Synergistic type microorganism.
6. according to the described Synergistic type microorganism Recompounded pesticide preparation method who is used for controlling plant diseases of claim 5, it is characterized in that: the bacteria culture media in the described step 1) is a kind of among YPGA, LB, NA, the KMB, and the inoculum in the step 3) is corresponding with step 1).
7. according to the described Synergistic type microorganism Recompounded pesticide preparation method who is used for controlling plant diseases of claim 5, it is characterized in that: described step 2) bacteria culture media is mixed with band prochloraz variable concentrations flat board, wherein the concentration of prochloraz is 5ug/ml, 10ug/ml, 20ug/ml....... increase progressively until 1000ug/ml; Bs-916 bacterial strain after the activation is moved in the flat board of 5ug/ml, treat that growth is good after, move into again in the flat board of 10ug/ml, and the like, up to the Bs-916 resistant strain that obtains on 1000ug/ml, to grow.
8. according to the described Synergistic type microorganism Recompounded pesticide preparation method who is used for controlling plant diseases of claim 7, it is characterized in that: cultivation temperature is 15-38 ℃ in the described step 3), shaken cultivation 22-55 hour acquisition culture fluid microbial inoculum.
9. the described according to Claim 8 Synergistic type microorganism Recompounded pesticide preparation method who is used for controlling plant diseases, it is characterized in that: the bacterial content of anti-prochloraz Bs-916 microbial inoculum is 2-5 * 10 in the described step 3)
10Cfu/ml.
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| Application Number | Priority Date | Filing Date | Title |
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| CNA200410014255XA CN1559228A (en) | 2004-03-09 | 2004-03-09 | Synergetic type microbial synergistic pesticide used for preventing and controlling plant diseases, and its prepn. method |
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| Application Number | Priority Date | Filing Date | Title |
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| CNA200410014255XA CN1559228A (en) | 2004-03-09 | 2004-03-09 | Synergetic type microbial synergistic pesticide used for preventing and controlling plant diseases, and its prepn. method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101985608A (en) * | 2010-10-25 | 2011-03-16 | 江苏省农业科学院 | Bacillus amyloliquefaciens strain and application thereof |
| CN102715193A (en) * | 2011-11-01 | 2012-10-10 | 江苏省农业科学院 | Bacillus subtilis sf-628 and prochloraz-manganese chloride complex compounded bactericide and use thereof |
| CN103548594A (en) * | 2013-10-28 | 2014-02-05 | 兰州大学 | Method for controlling Codonopsis pilosula yield and metabolite accumulation by utilizing bacteriabeneficial beneficial bacteria |
-
2004
- 2004-03-09 CN CNA200410014255XA patent/CN1559228A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101985608A (en) * | 2010-10-25 | 2011-03-16 | 江苏省农业科学院 | Bacillus amyloliquefaciens strain and application thereof |
| CN102715193A (en) * | 2011-11-01 | 2012-10-10 | 江苏省农业科学院 | Bacillus subtilis sf-628 and prochloraz-manganese chloride complex compounded bactericide and use thereof |
| CN102715193B (en) * | 2011-11-01 | 2013-10-30 | 江苏省农业科学院 | Bacillus subtilis sf-628 and prochloraz-manganese chloride complex compounded bactericide and use thereof |
| CN103548594A (en) * | 2013-10-28 | 2014-02-05 | 兰州大学 | Method for controlling Codonopsis pilosula yield and metabolite accumulation by utilizing bacteriabeneficial beneficial bacteria |
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