CN1556275A - Method of treating wool using composite protease directed against wool structure and dyestuff characteristics - Google Patents
Method of treating wool using composite protease directed against wool structure and dyestuff characteristics Download PDFInfo
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Abstract
A process for treating wool by use of different composite enzymes according to wool structure and dye characteristics includes ecological pretreating with H2O2 or disulfide-bond isomerase and modifying wool by the composite enzyme consisting of two or more different proteinases. It can realize low-temp dyeing and increase the dye exhausting rare, dyeing rate, and color fastness.
Description
Technical field:
The present invention relates to a kind of biological complex enzyme and handle the method for wool.Especially relate to a kind of method of handling wool at the compound protease of wool structure and dye characteristic.Belong to the textile dyeing and finishing technical field.
Technical background:
Along with the raising of people's living standard and the enhancing of environmental consciousness, people more and more tend to use the natural environmental-protective product and pay attention to cleaning of production manufacture process.In woolen industry, use biology enzyme to handle wool to reach various modification purpose and to use natural dye dying just complying with this requirement.The environmentally friendly property from the recyclability of raw material to production process and the natural and health of final products all show the sustainability and the good prospect of this production process.But only considered biology enzyme specificity in a big way when using biology enzyme to handle wool now mostly, as protease (catalytic degradation peptide bond), lipase (catalytic degradation ester bond), commentaries on classics glutaminase (peptide bond that protease is cut off reconnects) etc.; Specificity for wool has only been considered more rough aspect: mainly be made of protein as wool fibre, also have part lipoid (cell membrane and intercellular substance) etc.; Handle the back for enzyme to aspect the influence of dyeing property, only considering that biology enzyme handles the back wool modifying, increasing the diffusion admittance of dyestuff, reducing dyeing temperature, the dye exhausting rate when having improved low temperature dyeing.These all do not take the concrete composition of wool and the character of dyestuff into consideration, and this combination is very important.As everyone knows, wool belongs to protein fibre, mainly is made of amino acid.20 seed amino acids that constitute protein can be divided into 3 classes by its polarity: polar amino acid, intermediate polarity amino acid and nonpolar amino acid.Wherein polar amino acid comprises acidity, alkalescence and amide-type amino acid, is hydrophilic amino acid; Nonpolar amino acid comprises most of aliphatic amino acids and aromatic amino acid, is hydrophobic amino acid.And the kind of dyestuff and character are more, hydrophilic, hydrophobic, acid and basic-dyeable fibre etc. are also arranged, the selectivity of utilizing Protease Treatment is the partial amino-acid group of catalytic degradation wool targetedly, can help improving to greatest extent wool to the absorption of dyestuff with combine fastness and uniformity, this is significant to guaranteeing road, back dyeing quality.
New intension is paid attention to and given to natural dye being applied in weaving today again: back to nature, health care, environmental protection, sustainable development come from natural unique color and the novelty that conforms to no conventional pattern.Based on same reason---the composition of natural dye is impure and make its dye exhausting rate far below pure synthetic dyestuffs, when dying dark color more so.The costliness of comparing its price in addition with common synthetic dyestuffs makes the raising wool seem even more important to the exhaustion rate of natural dye, and biology enzyme processing wool can make the dyeing temperature of wool reduce greatly, dyeing rate is accelerated greatly, and the dye exhausting rate of natural dye is significantly improved.So both energy-conservation, save cost, guaranteed the dyeing condition of milder again, to the damage of wool or fabric, have numerous advantages when also having reduced simultaneously dyeing.
Summary of the invention:
The object of the present invention is to provide a kind of method of utilizing the catalysis selectivity of different protease at the compound protease processing wool of wool structure and dye characteristic, to reach low temperature dyeing and to improve dye exhausting rate to greatest extent and dyeing rate and dyefastness.
The object of the present invention is achieved like this: a kind of compound protease at wool structure and dye characteristic is handled the method for wool, is characterized in wool is carried out ecological pre-treatment and complex enzyme wool modifying:
The first step, the ecological pre-treatment of hydrogen peroxide, treatment conditions: hydrogen peroxide 5～40ml/L, the stabilizing agent sodium pyrophosphate 0.1～2g/L of hydrogen peroxide, main component is the heavy percentage of nonionic surface active agent peregal 0.1～2% cloth of polyoxyethylene aliphatic alcohol ether, pH=7～8,20 ℃～70 ℃ of temperature, time 10～60min; bath raio 1: 20～40, or: disulfide bond isomerase pre-treatment, treatment conditions: pH=7～8; 30 ℃～60 ℃ of temperature; time 10～30min, the heavy percentage of disulfide bond isomerase consumption 0.5～1.5% cloth, bath raio 1: 20～50;
The wool structure mainly is made up of following three parts: (1) scale layer, (2) cortical layer, (3) intercellular substance.And scale layer is divided into the outer A layer of scale, the outer B layer of scale and scale internal layer.The sulfur content of each part is all variant, and wherein the outer A layer sulfur content of scale is 35%, and the outer B layer of scale is 12%, and the scale internal layer is 3%; And the intercellular substance sulfur content only is 1%.In wool fibre, the disulfide bond of the big intermolecular formation of the high more then wool fibre of sulfur content is (S-S-) many more, molecule and intermolecular and intramolecular crosslinked many more then, the structure of this part is tight more hard, and protease is difficult for entering its inside configuration and carries out catalyzing hydrolysis.The ecological pre-treatment of hydrogen peroxide is to improve enzyme to handle predictable, reappearance and inhomogeneity ecological pre-treating method, the oxidation pre-treatment of hydrogen peroxide can opened disulfide bond, makes sulfur-containing amino acid such as cystine, methionine etc. in the wool be oxidized to cysteic acid (SO
3H/-SO
3 -), thereby make protease easily enter scale layer and act on wool fibre more equably, improve uniformity, stability and controllability that enzyme is handled, and reduce the wool strength loss.
The key of disulfide bond isomerase effect is the exchange reaction of catalysis sulfydryl and disulfide bond, it be enzyme be again molecular chaperones, combine the efficient that improves enzyme with substrate in quasi-molecule companion's mode.Molecular chaperones is that a class does not have correlation but the protein of common function is arranged on sequence, helps other structures that contain polypeptide to finish correct assembling in cell, but does not constitute the constituent of this protein or polypeptide; Can reset to impel oligomeric peptide white matter recurring structure with the protein interaction of native conformation.The character of disulfide bond isomerase molecular chaperones helps substrate unfolding (opened disulfide bond) or folding (the reconstruction disulfide bond) of enzyme, and the work that provides an efficient to improve greatly for disulfide bond isomerase has mechanism.
Second step, complex enzyme wool modifying
Complex enzyme wool modifying method of the present invention has two kinds:
First kind, at the hydrophilic natural dyestuff, adopt two kinds in protease or several Combined Processing wools of selectivity degraded hydrophobic amino acid group, treatment conditions: in pH=7～8, under 30～60 ℃ of conditions of temperature, handle wool 10～60min, the consumption of complex enzyme: the heavy percentage of 0.4%～4.0% cloth; bath raio 1: 20～50; compound protease of this selectivity degraded hydrophobic amino acid group has microbe-derived protease; the protease of animal origin; the protease of plant origin; wherein microbe-derived protease has neutral to faintly acid bacillus protein enzyme; the protease of animal origin has chymotrypsin; Carboxypeptidase A; the protease of plant origin has papain; ficin, and the bacillus protein enzyme has hay bacillus again; bacillus licheniformis; bacillus pumilus; Bacillus cercus.
The amino acid of composition wool can be divided into 7 classes according to the difference of its side chain character: aliphatic amino acid, aromatic amino acid, sulfur-containing amino acid, pure amino acid, amide-type amino acid and acidic amino acid and basic amino acid.Wherein aliphatic amino acid and aromatic amino acid belong to hydrophobic amino acid mostly, as valine, leucine, isoleucine, phenylalanine, tryptophan etc.For making wool better affinity be arranged to hydrophilic dye (as lotus leaf), can utilize the selectivity of catalytic degradation of protease to remove hydrophobic amino acid in the wool protein, handle as adopting alkalescent subtilopeptidase A or chymotrypsin.Both all belong to serine protease, alkalescent subtilopeptidase A selectivity degraded tyrosine, tryptophan and phenylalanine wherein, strong-hydrophobicity amino acid such as chymotrypsin protein enzyme specificity degraded tyrosine, tryptophan and phenylalanine, valine, leucine, isoleucine.And the selectivity characteristics of papain are the catalytic degradation caseins, and macro-molecular protein is degraded to the micromolecule albuminoid, papain belongs to thiol protease, derive from plant, the membrane structure and the cuticula that compare wool with serine protease have better permeability, therefore can work in coordination with the processing of subtilopeptidase A or chymotrypsin, and more hydrophilic radical is provided.Under the acting in conjunction of two or more enzymes, softened the scale layer of wool, the passage that on the one hand provides polychromatophilia material more to enter provides more groups that can combination dye on the other hand, improved dye exhausting rate and dyeing rate greatly, and COLOR FASTNESS improves slightly also.
Second kind, at the hydrophobicity natural dye, adopt two kinds in protease or several Combined Processing wools of selectivity degraded hydrophilic amino acid groups, treatment conditions: in pH=7～8, under 30～60 ℃ of conditions of temperature, handle wool 10～60min, the consumption of complex enzyme: the heavy percentage of 0.4%～4.0% cloth; bath raio 1: 20～50; compound protease of this selectivity degraded hydrophilic amino acid groups has microbe-derived protease; the protease of animal origin; the protease of plant origin; wherein microbe-derived protease has neutral to alkalescent actinomyces protease; mold protease; rice black mold; the protease of animal origin has trypsase; endopeptidase; intestines coagulate enzyme; protaminase, and the protease of plant origin has papain; ficin; bromelain.
Main component as the natural dye alkannin is shikonin, and is water insoluble, is dissolved in organic solvent, and its structural formula is:
Because of the Lithospermum hydrophobic dye, when dyeing, be prone to the dyeing flower phenomenon, need add dispersant.At the characteristic of alkannin, adopt trypsase, bromelain and papain Combined Processing wool.Wherein trypsase selectivity degrading alkaline amino acid such as arginine and lysine, bromelain enzyme specificity degraded arginine, lysine, tyrosine and glycine; Papain as previously mentioned, because the tyrosine side chain has phenolic hydroxyl group, its pKa value is 10.5, and hydrophobicity does not resemble the phenylalanine strong, and because papain to the synergy of other enzymes, makes it no matter hydrophobicity natural dye or hydrophilic natural dyestuff all to be suitable for.
After complex enzyme was handled wool, modified wool adopted low temperature dyeing technique, owing to removed and the not good compatible hydrophilic amino acid of alkannin, the even dyeing nature, soft pleasing.
The specific embodiment:
Embodiment 1: the wool pre-treatment---the hydrogen peroxide pre-treatment
Hydrogen peroxide pre-treatment wool fabric: 30% hydrogen peroxide 20ml/L, sodium pyrophosphate 1.25g/L, paregal O 0.5% (the heavy percentage of cloth), PH=7.5,45 ℃ of temperature, bath raio 1: 20, time 30min.
Embodiment 2: the wool pre-treatment---the hydrogen peroxide pre-treatment
Hydrogen peroxide pre-treatment wool fabric: 30% hydrogen peroxide 5ml/L, sodium pyrophosphate 0.1g/L, paregal O 0.1% (the heavy percentage of cloth), PH=7,20 ℃ of temperature, bath raio 1: 40, time 60min.
Embodiment 3: the wool pre-treatment---the hydrogen peroxide pre-treatment
Hydrogen peroxide pre-treatment wool fabric: 30% hydrogen peroxide 40ml/L, sodium pyrophosphate 2g/L, paregal O 2% (the heavy percentage of cloth), PH=8,70 ℃ of temperature, bath raio 1: 30, time 10min.
Embodiment 4: the wool pre-treatment---the disulfide bond isomerase pre-treatment
Disulfide bond isomerase pre-treatment wool fabric: PH=7, purifying also guarantees the disulfide bond isomerase 0.5% (cloth heavy percentage) of vigor, 60 ℃ of temperature, bath raio 1: 20, time 10min.
Embodiment 5: the wool pre-treatment---and disulfide bond isomerase pre-treatment disulfide bond isomerase pre-treatment wool fabric: PH=7.5, purifying also guarantees the disulfide bond isomerase 1% (cloth heavy percentage) of vigor, 45 ℃ of temperature, bath raio 1: 30, time 30min.
Embodiment 6: the wool pre-treatment---the disulfide bond isomerase pre-treatment
Disulfide bond isomerase pre-treatment wool fabric: PH=8, purifying also guarantees the disulfide bond isomerase 1.5% (cloth heavy percentage) of vigor, 30 ℃ of temperature, bath raio 1: 50, time 20min.
Embodiment 7: the modification of wool complex enzyme-and at the hydrophilic natural dyestuff
1) hydrogen peroxide pre-treatment wool fabric: with embodiment 1.
2) complex enzyme is handled wool fabric: pH=7.5, gets purifying and guarantees the papain 0.2% (the heavy percentage of cloth) of vigor, subtilopeptidase A 0.8% (the heavy percentage of cloth), 45 ℃ of temperature, time 30min, bath raio 1: 30.
Embodiment 8: the modification of wool complex enzyme-and at the hydrophilic natural dyestuff
1) hydrogen peroxide pre-treatment wool fabric: with embodiment 2.
2) complex enzyme is handled wool fabric: pH=7, gets purifying and guarantees the ficin 0.5% (the heavy percentage of cloth) of vigor, chymotrypsin 1.0% (the heavy percentage of cloth), 30 ℃ of temperature, time 60min, bath raio 1: 20.
Embodiment 9: the modification of wool complex enzyme-and at the hydrophilic natural dyestuff
1) hydrogen peroxide pre-treatment wool fabric: with embodiment 3.
2) complex enzyme is handled wool fabric: pH=8, get purifying and guarantee the papain 1.0% (cloth heavy percentage) of vigor, bacillus licheniformis protease 1.0% (the heavy percentage of cloth), chymotrypsin 0.2% (the heavy percentage of cloth), Carboxypeptidase A 0.2% (the heavy percentage of cloth), 60 ℃ of temperature, time 10min, bath raio 1: 50.
Embodiment 10: the modification of wool complex enzyme-and at the hydrophilic natural dyestuff
1) disulfide bond isomerase pre-treatment wool fabric: with embodiment 4.
2) complex enzyme is handled wool fabric: pH=7, gets purifying and guarantees the ficin 0.2% (the heavy percentage of cloth) of vigor, Carboxypeptidase A 1.0% (the heavy percentage of cloth), 30 ℃ of temperature, time 30min, bath raio 1: 30.
Embodiment 11: the modification of wool complex enzyme-and at the hydrophilic natural dyestuff
1) disulfide bond isomerase pre-treatment wool fabric: with embodiment 5.
2) complex enzyme is handled wool fabric: pH=7, gets purifying and guarantees the papain 0.5% (the heavy percentage of cloth) of vigor, Carboxypeptidase A 0.2% (the heavy percentage of cloth), bacillus pumilus protease 1% (the heavy percentage of cloth), 60 ℃ of temperature, time 10min, bath raio 1: 20.
Embodiment 12: the modification of wool complex enzyme-and at the hydrophilic natural dyestuff
1) disulfide bond isomerase pre-treatment wool fabric: with embodiment 6.
2) complex enzyme is handled wool fabric: pH=8, gets purifying and guarantees the ficin 1% (the heavy percentage of cloth) of vigor, Bacillus cercus proteinase-10 .2% (the heavy percentage of cloth), 45 ℃ of temperature, time 60min, bath raio 1: 50.
Embodiment 13: the modification of wool complex enzyme-and at the hydrophobicity natural dye
1) disulfide bond isomerase pre-treatment wool fabric: with embodiment 4.
2) complex enzyme is handled wool fabric: pH=7, gets purifying and guarantees the bromelain 0.2% (the heavy percentage of cloth) of vigor, trypsase 1.0% (the heavy percentage of cloth), 45 ℃ of temperature, time 30min, bath raio 1: 30.
Embodiment 14: the modification of wool complex enzyme-and at the hydrophobicity natural dye
1) disulfide bond isomerase pre-treatment wool fabric: with embodiment 5.
2) complex enzyme is handled wool fabric: pH=7, gets purifying and guarantees the papain 0.5% (the heavy percentage of cloth) of vigor, trypsase 0.2% (the heavy percentage of cloth), rice black mold 0.5% (the heavy percentage of cloth), 60 ℃ of temperature, time 10min, bath raio 1: 20.
Embodiment 15: the modification of wool complex enzyme-and at the hydrophobicity natural dye
1) disulfide bond isomerase pre-treatment wool fabric: with embodiment 6.
2) complex enzyme is handled wool fabric: pH=7, gets purifying and guarantees the ficin 1% (the heavy percentage of cloth) of vigor, endopeptidase 1.0% (the heavy percentage of cloth), 45 ℃ of temperature, time 10min, bath raio 1: 50.
Embodiment 16: the modification of wool complex enzyme-and at the hydrophobicity natural dye
1) hydrogen peroxide pre-treatment wool fabric: with embodiment 1.
2) complex enzyme is handled wool fabric: pH=7, gets purifying and guarantees the bromelain 1% (the heavy percentage of cloth) of vigor, protaminase 1.0% (the heavy percentage of cloth), 45 ℃ of temperature, time 30min, bath raio 1: 30.
Embodiment 17: the modification of wool complex enzyme-and at the hydrophobicity natural dye
1) hydrogen peroxide pre-treatment wool fabric: with embodiment 2.
2) complex enzyme is handled wool fabric: pH=7, gets purifying and guarantees the papain 0.2% (the heavy percentage of cloth) of vigor, trypsase 0.2% (the heavy percentage of cloth), actinomyces proteinase-10 .5% (the heavy percentage of cloth), 60 ℃ of temperature, time 10min, bath raio 1: 20.
Embodiment 18: the modification of wool complex enzyme-and at the hydrophobicity natural dye
1) hydrogen peroxide pre-treatment wool fabric: with embodiment 3.
2) complex enzyme is handled wool fabric: pH=7, gets purifying and guarantees the ficin 0.2% (the heavy percentage of cloth) of vigor, and intestines coagulate enzyme 1.0% (the heavy percentage of cloth), 45 ℃ of temperature, time 10min, bath raio 1: 50.
Embodiment 19: the complex enzyme modification---hydrophilic natural dyestuff lotus leaf dyeing
1) pigment extracts: lotus leaf adds 10 times of soft water and boils and boil 2h, filter stoste, spray-dried again chromatoplast.
2) ecological preliminary treatment: embodiment 7 simultaneously.
3) dyeing:
A) the aluminium matchmaker handles: the wool fabric that enzyme was handled adds 5% (cloth weight percentage) aluminum sulfate and carries out mordant dyeing, 80 ℃ of temperature, and 2 ℃/min of heating rate is incubated 30min, bath raio 1: 20.
B) lotus leaf dyeing: get 1) gained chromatoplast 3% (the heavy percentage of cloth) dyeing, the beginning is dyed 30 ℃ of temperature, and 0.5 ℃/min of heating rate is to 80 ℃ of insulation 1h, bath raio 1: 20.
Embodiment 20: the complex enzyme modification---hydrophobicity natural dye Asian puccoon dyeing
1) pigment extracts: Asian puccoon adds 10 times of alcohol-pickled 24h, filter stoste, the stoste dilution can be dyeed.
2) ecological preliminary treatment: with embodiment 13.
3) dyeing:
A) the aluminium matchmaker handles: the wool fabric that enzyme was handled adds 5% (cloth weight percentage) aluminum sulfate and carries out mordant dyeing, 60 ℃ of temperature, and 2 ℃/min of heating rate is incubated 30min, bath raio 1: 20.
B) Asian puccoon dyeing: get 1) gained stoste 100% (the heavy percentage of cloth) dyeing, the beginning is dyed 30 ℃ of temperature, heating rate: 0.5 ℃/min, to 60 ℃ of insulation 1h, bath raio 1: 40.
Embodiment 21: the complex enzyme modification---hydrophobicity natural dye Asian puccoon dyeing
1) pigment extracts: Asian puccoon adds 20 times of alcohol-pickled 36h, filter stoste, the stoste dilution can be dyeed.
2) ecological preliminary treatment: with embodiment 13.
3) dyeing: with embodiment 20 3)
Embodiment 22: composite enzyme modification---hydrophobicity natural dye Asian puccoon dyeing
1) pigment extracts: Asian puccoon adds 30 times of alcohol-pickled 48h, filter stoste, the stoste dilution can be dyeed.
2) ecological preliminary treatment: with embodiment 14.
3) dyeing: with embodiment 20 3)
Claims (9)
1, a kind of compound protease at wool structure and dye characteristic is handled the method for wool, it is characterized in that wool is carried out ecological pre-treatment and complex enzyme wool modifying:
The first step, the ecological pre-treatment of hydrogen peroxide, treatment conditions: hydrogen peroxide 5～40ml/L, the stabilizing agent sodium pyrophosphate 0.1～2g/L of hydrogen peroxide, main component is the heavy percentage of nonionic surface active agent peregal 0.1～2% cloth of polyoxyethylene aliphatic alcohol ether, pH=7～8,20 ℃～70 ℃ of temperature, time 10～60min; bath raio 1: 20～40, or: disulfide bond isomerase pre-treatment, treatment conditions: pH=7～8; 30 ℃～60 ℃ of temperature; time 10～30min, the heavy percentage of disulfide bond isomerase consumption 0.5～1.5% cloth, bath raio 1: 20～50;
Second step, complex enzyme wool modifying,
At the hydrophilic natural dyestuff, adopt two kinds in protease or several Combined Processing wools of selectivity degraded hydrophobic amino acid group, treatment conditions: in pH=7～8, under 30～60 ℃ of conditions of temperature, handle wool 10～60min, the consumption of complex enzyme: the heavy percentage of 0.4%～4.0% cloth; bath raio 1: 20～50; compound protease of this selectivity degraded hydrophobic amino acid group has microbe-derived protease; the protease of animal origin; the protease of plant origin; wherein microbe-derived protease has neutral to faintly acid bacillus protein enzyme; the protease of animal origin has chymotrypsin; Carboxypeptidase A; the protease of plant origin has papain; ficin; the bacillus protein enzyme has hay bacillus again; bacillus licheniformis; bacillus pumilus; Bacillus cercus, or:
At the hydrophobicity natural dye, adopt two kinds in protease or several Combined Processing wools of selectivity degraded hydrophilic amino acid groups, treatment conditions: in pH=7～8, under 30～60 ℃ of conditions of temperature, handle wool 10～60min, the consumption of complex enzyme: the heavy percentage of 0.4%～4.0% cloth; bath raio 1: 20～50; compound protease of this selectivity degraded hydrophilic amino acid groups has microbe-derived protease; the protease of animal origin; the protease of plant origin; wherein microbe-derived protease has neutral to alkalescent actinomyces protease; mold protease; rice black mold; the protease of animal origin has trypsase; endopeptidase; intestines coagulate enzyme; protaminase, and the protease of plant origin has papain; ficin; bromelain.
2, a kind of method of handling wool at the compound protease of wool structure and dye characteristic according to claim 1, it is characterized in that at the hydrophilic natural dyestuff, adopt the compound protease and the consumption of selectivity degraded hydrophobic amino acid group to be: the heavy percentage of papain 0.2%～1.0% cloth, the heavy percentage of subtilopeptidase A 0.2%～1.0% cloth.
3, a kind of method of handling wool at the compound protease of wool structure and dye characteristic according to claim 1, it is characterized in that at the hydrophilic natural dyestuff, adopt the compound protease and the consumption of selectivity degraded hydrophobic amino acid group to be: the heavy percentage of ficin 0.2%～1.0% cloth, the heavy percentage of chymotrypsin or Carboxypeptidase A 0.2%～1.0% cloth.
4, a kind of method of handling wool at the compound protease of wool structure and dye characteristic according to claim 1, it is characterized in that at the hydrophilic natural dyestuff, adopt the compound protease and the consumption of selectivity degraded hydrophobic amino acid group to be: the heavy percentage of papain 0.2%～1.0% cloth, the heavy percentage of bacillus licheniformis proteinase-10 .2%～1.0% cloth, the heavy percentage of chymotrypsin 0.2%～1.0% cloth, the heavy percentage of Carboxypeptidase A 0.2%～1.0% cloth.
5, a kind of method of handling wool at the compound protease of wool structure and dye characteristic according to claim 1, it is characterized in that at the hydrophilic natural dyestuff, adopt the compound protease and the consumption of selectivity degraded hydrophobic amino acid group to be: the heavy percentage of papain 0.2%～1.0% cloth, the heavy percentage of Carboxypeptidase A 0.2%～1.0% cloth, the heavy percentage of bacillus pumilus proteinase-10 .2%～1.0% cloth.
6, a kind of method of handling wool at the compound protease of wool structure and dye characteristic according to claim 1, it is characterized in that at the hydrophilic natural dyestuff, adopt the compound protease and the consumption of selectivity degraded hydrophobic amino acid group to be: the heavy percentage of ficin 0.2%～1.0% cloth, the heavy percentage of Bacillus cercus proteinase-10 .2%～1.0% cloth.
7, a kind of method of handling wool at the compound protease of wool structure and dye characteristic according to claim 1, it is characterized in that at the hydrophobicity natural dye, adopt the compound protease and the consumption of selectivity degraded hydrophilic amino acid groups to be: the heavy percentage of bromelain 0.2%～1.0% cloth, the heavy percentage of trypsase or protaminase 0.2%～1.0% cloth.
8, a kind of method of handling wool at the compound protease of wool structure and dye characteristic according to claim 1, it is characterized in that at the hydrophobicity natural dye, adopt the compound protease and the consumption of selectivity degraded hydrophilic amino acid groups to be: the heavy percentage of ficin 0.2%～1.0% cloth, endopeptidase or intestines coagulate the heavy percentage of enzyme 0.2%～1.0% cloth.
9, a kind of method of handling wool at the compound protease of wool structure and dye characteristic according to claim 1, it is characterized in that at the hydrophobicity natural dye, adopt the compound protease and the consumption of selectivity degraded hydrophilic amino acid groups to be: the heavy percentage of papain 0.2%～1.0% cloth, the heavy percentage of trypsase 0.2%～1.0% cloth, the heavy percentage of rice black mold or actinomyces proteinase-10 .2%～1.0% cloth.
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