CN1555690A - European Asia variety grape seedless treatment method - Google Patents
European Asia variety grape seedless treatment method Download PDFInfo
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- CN1555690A CN1555690A CNA2003101216166A CN200310121616A CN1555690A CN 1555690 A CN1555690 A CN 1555690A CN A2003101216166 A CNA2003101216166 A CN A2003101216166A CN 200310121616 A CN200310121616 A CN 200310121616A CN 1555690 A CN1555690 A CN 1555690A
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- vitis vinifera
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Abstract
A method for culturing the non-seed Ouya grape includes such steps as preparing the solution from streptomycin, gibberellin and distilled water, immersing the bunches of flower in the solution from the 6 days before bloosoming to the 3 days after blossoming, preparing the solution of gibberellin or forchlorfenuron, and treating the grapes with the solution about 14 days after blossoming. Its non-seed ratio can reach more than 95%.
Description
Technical field:
The present invention relates to a kind of vitis vinifera denuclearization processing method, be used to cultivate seedless bulky grain vitis vinifera, belong to farm-forestry crop culture technique field.
Background technology:
Currant does not have when edible and tells the loaded down with trivial details of seed, is subjected to consumer's welcome deeply.But the germ plasm resource of present natural seedless variety is also not too abundant, and natural currant generally shows fruit grain shortcoming less than normal, can not satisfy the demand of consumer to seedless big grain grape.The effective technology measure that addresses the above problem is that the nuclear grape variety that has of will cultivate adopts the denuclearization technology, makes it produce seedless big grain grape.And the key of denuclearization technology promptly is the selection and the operation technique of kernal eliminating agent.Induce the no seed production of nuclear kind with gibberellic acid (GAs), at first achieve success on " rose flavored juice (Delaware) " of Ribenshanli County grape in nineteen fifty-seven, and the application of on grape varieties such as " pioneer (Pione) ", " bud rose A (Muscat berry A) ", succeeding.Nearly 10 over several years, many researchers of China have carried out gibberellin (non-gibberellic acid) and have induced the grape kernal eliminating Study on Technology, and develop the product of " kernal eliminating agent ", " the seed spirit disappears " and so on, but still there are not the successful product or the technology of large scale application so far.
The kernal eliminating agent of taking a broad view of domestic and international grape kernal eliminating culture technique and being adopted still has 3 deficiencies at present: (1) though better to the seedless effect of inducing of Franco-american grape variety, bad to the effect that the vitis vinifera denuclearization is handled; (2) costing an arm and a leg of seedless derivant, too high (about 125 yuan of the price of present domestic chemical reagent shop gibberellic acid/100mg), under the lower situation of China's grape price, be difficult to promote of cost; (3) effectively the processing time is short, and general kind has only 3 days, and large-area applications is difficulty very.
Summary of the invention:
The objective of the invention is to provides a kind of vitis vinifera denuclearization processing method at present domestic and international grape kernal eliminating culture technique above shortcomings part, makes the nuclear vitis vinifera that has of existing cultivation produce seedless big grain grape.
For realizing such purpose, the invention provides the vitis vinifera kernal eliminating agent and use kernal eliminating agent carries out the denuclearization processing to vitis vinifera method.Vitis vinifera kernal eliminating agent of the present invention is formed by streptomycin sulphate and gibberellin and distilled water mixed preparing, is specially: 200 mg/litre streptomycin sulphates (MS) and 50 mg/litre gibberellin (GA
s) mixed solution, be MS 200mg/L+GA
s50mg/L.Compound method is: take by weighing 200 milligrams in streptomycin sulphate pulvis with electronic balance, add the dissolving of 20-30 ml distilled water, all be transferred to 1000 milliliters of volumetric flasks; Take by weighing 50 milligrams in gibberellin pulvis with electronic balance again, add the dissolving of 10-20 ml ethanol, all be transferred to 1000 milliliters of volumetric flasks; Last adding distil water to 1000 milliliter volumetric flask is settled to 1000 milliliters and gets final product.
Vitis vinifera denuclearization processing method is: adopt vitis vinifera kernal eliminating agent and gibberellin (GAs) or forchlorfenuron (CPPU) as medicament, vitis vinifera is carried out twice processing.Handle for the first time and spend preceding 6 days in Sheng and spend back 3 days, use the vitis vinifera kernal eliminating agent, i.e. streptomycin sulphate (MS) 200mg/L+ gibberellin (GA to containing
s) mixed solution of 50mg/L dips the grape flower fringe.Handle in Sheng for the second time and spend back about 14 days, dip or spray the fruit grain of cloth denuclearization with gibberellin (GAs) 25～50mg/L or forchlorfenuron (CPPU) 5～10mg/L solution.
Applicable variety of the present invention is: vitis vinifera kinds such as " rose ", " bright red ", " black rose ", " Italy ".
Vitis vinifera kernal eliminating agent preparation of the present invention is easy, and effect is obvious.Spending preceding 6 days in Sheng spends back 3 days and is used to dip vitis vinifera flower (really) fringe to containing, cooperating gibberellin or forchlorfenuron solution to spend back about 14 days in Sheng handles the fruit grain, can make the seedless rate of vitis vinifera up to more than 95%, fruit grain transverse diameter and single fruit weight all near or surpass contrast, soluble solid (TSS) content, pericarp anthocyanidin content (O.D.535nm) improve 2-6 ° of Brix and 20-70% successively, effectively reach 9-10 days the seedless period of inducing, no nuclear technique than Japan prolongs 6-7 days, helps large-area applications.Kernal eliminating agent of the present invention is cheap, and the application cost on every mu of ground approximately is 1/30～1/80 of a day present technique.Kernal eliminating agent residual in fruit grain grows gradually and reduces along with the fruit grain, and the content＜1mg/Kg in the mellow fruit grain is the residual dosage of a safety.Under the physical behavior of pulvis, sustainable at least 4 years of the term of validity of kernal eliminating agent.
Embodiment:
1, the preparation of kernal eliminating agent: vitis vinifera kernal eliminating agent prescription provided by the invention is: 200 mg/litre streptomycin sulphates (MS) and 50 mg/litre gibberellin (GA
s) mixed solution, be MS 200mg/L+GA
s50mg/L.Compound method is: take by weighing 200 milligrams in streptomycin sulphate pulvis (Shanghai the 4th pharmaceutical factory produces, and purity is 700,000 units) with electronic balance, add the dissolving of 20-30 ml distilled water, all be transferred to 1000 milliliters of volumetric flasks; Take by weighing 50 milligrams in gibberellin pulvis (solvent factory in Shanghai produces, and purity is 99%) with electronic balance again, add the dissolving of 10-20 ml ethanol, all be transferred to 1000 milliliters of volumetric flasks; Last adding distil water to 1000 milliliter volumetric flask is settled to 1000 milliliters and gets final product.
2, use kernal eliminating agent vitis vinifera is carried out the denuclearization processing: (is example with " rose " kind)
Handle for the first time: contain at " rose " grape and spend preceding 6 days and spend back 3 days, dip muscat grape with " vitis vinifera kernal eliminating agent " of the present invention solution and spend fringe, time 5-10 second to containing.Consequently " vitis vinifera kernal eliminating agent " handled and induced blastular to grow undesired and after fertilization seeds abortion, thereby produces seedless fruit, and seedless rate is up to more than 95%.
Handle for the second time: contain at " rose " grape and spend back about 14 days and dip or spray the seedless fruit grain that cloth is handled generation for the first time with gibberellin (GAs) 25～50mg/L solution or forchlorfenuron (CPPU) 5～10mg/L solution, consequently the seedless fruit grain fully expands during fruit maturation, its fruit grain size and single fruit weight are all approaching or surpass contrast, and the soluble solid of fruit (TSS) content, pericarp anthocyanidin content (O.D.535nm) improve 2-6 ° of Brix and 20-70% successively.
Claims (3)
1, a kind of vitis vinifera kernal eliminating agent is characterized in that being formed by 200 mg/litre streptomycin sulphates and 50 mg/litre gibberellin and distilled water mixed preparing.
2, a kind of vitis vinifera denuclearization processing method, it is characterized in that at twice vitis vinifera being handled, handle for the first time and spend preceding 6 days in Sheng and spend back 3 days to containing, use the vitis vinifera kernal eliminating agent that forms by 200 mg/litre streptomycin sulphates and 50 mg/litre gibberellin and distilled water mixed preparing to dip the grape flower fringe, handle in Sheng for the second time and spend back 14 days, dip or spray the fruit grain of cloth denuclearization with gibberellin 25～50mg/L or forchlorfenuron 5～10mg/L solution.
3, vitis vinifera denuclearization processing method as claimed in claim 2 is characterized in that described grape variety is " rose ", " bright red ", " black rose ", " Italy " vitis vinifera kind.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2003101216166A CN1555690A (en) | 2003-12-30 | 2003-12-30 | European Asia variety grape seedless treatment method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2003101216166A CN1555690A (en) | 2003-12-30 | 2003-12-30 | European Asia variety grape seedless treatment method |
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| CN1555690A true CN1555690A (en) | 2004-12-22 |
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| CNA2003101216166A Pending CN1555690A (en) | 2003-12-30 | 2003-12-30 | European Asia variety grape seedless treatment method |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100384325C (en) * | 2006-06-07 | 2008-04-30 | 南京农业大学 | Tech. for culturing denulcearized Weike grape |
| CN100502660C (en) * | 2006-08-04 | 2009-06-24 | 广东省农业科学院植物保护研究所 | Fruits protection effervescence tablets, preparation method and use thereof |
| CN101278639B (en) * | 2008-05-29 | 2011-04-06 | 吕秀兰 | Technique for cultivating seedless grape |
| CN103250580A (en) * | 2013-05-06 | 2013-08-21 | 丁在喜 | Method for softening hard pomegranate seeds |
| CN103828682A (en) * | 2014-02-21 | 2014-06-04 | 沈金跃 | Big seedless grape cultivation method |
| CN105475063A (en) * | 2014-10-08 | 2016-04-13 | 肖卷忠 | Seedless cultivation method for Wink |
| CN106472140A (en) * | 2016-10-09 | 2017-03-08 | 曾兴华 | The method of the sweet jasmine grape kernal eliminating of induction |
| CN106688806A (en) * | 2017-01-03 | 2017-05-24 | 曾其彤 | Deseeding cultivation method of Kyoho grapes with seeds |
| CN114586563A (en) * | 2022-04-11 | 2022-06-07 | 河南省农业科学院园艺研究所 | Method for protecting fruit before flowering and expanding after flowering of sunshine rose grape |
| CN114651661A (en) * | 2022-03-15 | 2022-06-24 | 四川瑞象农业科技发展有限公司 | High-quality cultivation technology for sunshine roses |
-
2003
- 2003-12-30 CN CNA2003101216166A patent/CN1555690A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100384325C (en) * | 2006-06-07 | 2008-04-30 | 南京农业大学 | Tech. for culturing denulcearized Weike grape |
| CN100502660C (en) * | 2006-08-04 | 2009-06-24 | 广东省农业科学院植物保护研究所 | Fruits protection effervescence tablets, preparation method and use thereof |
| CN101278639B (en) * | 2008-05-29 | 2011-04-06 | 吕秀兰 | Technique for cultivating seedless grape |
| CN103250580A (en) * | 2013-05-06 | 2013-08-21 | 丁在喜 | Method for softening hard pomegranate seeds |
| CN103250580B (en) * | 2013-05-06 | 2015-04-22 | 丁在喜 | Method for softening hard pomegranate seeds |
| CN103828682A (en) * | 2014-02-21 | 2014-06-04 | 沈金跃 | Big seedless grape cultivation method |
| CN105475063A (en) * | 2014-10-08 | 2016-04-13 | 肖卷忠 | Seedless cultivation method for Wink |
| CN106472140A (en) * | 2016-10-09 | 2017-03-08 | 曾兴华 | The method of the sweet jasmine grape kernal eliminating of induction |
| CN106688806A (en) * | 2017-01-03 | 2017-05-24 | 曾其彤 | Deseeding cultivation method of Kyoho grapes with seeds |
| CN114651661A (en) * | 2022-03-15 | 2022-06-24 | 四川瑞象农业科技发展有限公司 | High-quality cultivation technology for sunshine roses |
| CN114586563A (en) * | 2022-04-11 | 2022-06-07 | 河南省农业科学院园艺研究所 | Method for protecting fruit before flowering and expanding after flowering of sunshine rose grape |
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