CN1552501A - Chromatographic column with axial compression fastener - Google Patents
Chromatographic column with axial compression fastener Download PDFInfo
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- CN1552501A CN1552501A CNA031335071A CN03133507A CN1552501A CN 1552501 A CN1552501 A CN 1552501A CN A031335071 A CNA031335071 A CN A031335071A CN 03133507 A CN03133507 A CN 03133507A CN 1552501 A CN1552501 A CN 1552501A
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- chromatographic column
- flange
- piston
- axial compression
- compression
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Abstract
A chromatographic column with axial compressing and fixing structure is composed of an empty chromatographic column and two end covers. It features that its additional axial compressing and fixing structure consists of a compressing flange with locating and sealing structure, a cylindrical rod matched with the inner surface of chromatographic column, a porous piston and the fastening screw bolts. The filler is filled in said empty cholumn. Its advantages are small size and low cost.
Description
Technical field:
The present invention relates to chromatographic technique, a kind of chromatographic column that has the compression fastener of being made up of compression flange, tubular bar and piston is provided especially.
Background technology:
The separating effect even, stable and chromatogram of packing density is closely related in the chromatographic column bed.For obtaining and keep all even stable of packing density, chromatogram circle generally adopts compress technique to eliminate space between filler, and relevant technology has radial compression, axial compression and compound compressed.Axial compression is to utilize mechanical pressure or hydraulic drive piston, the outstanding slurry of concentrating of aequum is compressed to the piston stop motion, and in elution process, remaining fixation pressure (" power axial compression ", Colin H, Hilaireau P, de Toumemire J 1990, LC-GC Intl 3:40), guarantee that filler is stable in the post bed, density is even or eliminate the space.At present, the most general with line shaft to the application of compress technique.
U.S. Pat P5,893,971 disclose a kind of filler fastener of being made up of piston and fastening screw, are furnished with holddown spring on the fastening screw, the nut coupling at fastening screw and chromatographic column upper end cover center.Chromatographic column is finished charging and is compressed on specialized equipment, and after installing the chromatographic column end cap of being furnished with fastening screw, screws fastening screw, and the spring force on the fastening screw is fastening with filler by piston.This Patent equipment needs special-purpose filler filling, compression set, and the space that fastening screw occupies can not make full use of, and is difficult to needing to be used for the sample separation (preparing chromatograph in industry) of sample on the solid.
For chromatographic column infant industry preparation chromatographic column particularly, have now powered compressor technology and device volume huge, cost an arm and a leg, and install and use inconvenience (USP5,893,971.USP 6,001,260).
1985 Rainin (USP 4,861, and 473.USP 5,674,455) developed the screw turncap powered compressor technique device at chromatographic column two ends: by filler in the screw turncap compression post at manual rotation two ends, prevent or eliminate the generation in space in the elution process.But the power that the used screw turncap of Rainin compression set provides is limited, is only applicable to diameter and is 10～80mm, the length chromatographic column less than 250mm.The inconvenience of last sample.Along with the particularly development of industrial preparative column of novel chromatographic column, above-mentioned technology can not be satisfied the demand.
Technology contents:
The objective of the invention is to overcome existing powered compressor fastener bulky, cost an arm and a leg and the limited defective of power that screw turncap compression fastener provides, provide a kind of have simple, effectively, the chromatographic column of convenient and economic compression tightening technology device.
For achieving the above object, the invention provides a kind of chromatographic column that has the axial compression fastener, chromatographic column end cap (6) by chromatogram void column (7) and two ends constitutes, it is characterized in that: increase by an axial compression fastener, form by compression flange (1), tubular bar (2), piston (3) and fastening bolt (5); Described compression flange (1) has location and the hermetically-sealed construction with chromatogram void column (7) flange coupling; Described tubular bar (2) is close to the tubular structure of chromatographic column inwall for the outer wall that slides in post; The porous structure of described piston (3) in post, sliding; The chromatogram void column is inserted filler in (7), filler top is followed successively by piston (3), tubular bar (2), compression flange (1), chromatographic column end cap (6), and fastening bolt (5) is fastenedly connected chromatographic column in aggregates with the flange of the flange of chromatogram void column (7), compression flange (1), chromatographic column end cap (6).
The present invention has in the chromatographic column of axial compression fastener, can also be equipped with hydraulic means (9) between described compression flange (1) and chromatographic column end cap (6).
The present invention has in the chromatographic column of axial compression fastener, described can the sealing by O-ring seal (4) and compression flange (1), chromatographic column inwall and piston (3) at tubular bar two ends.Perhaps tubular bar (2) and compression flange (1) can be made as a whole (1-1).The tubular bar should have enough wall thickness, transmits and bear the required physical strength of pressure to satisfy.
The present invention has in the chromatographic column of axial compression fastener, and described piston (3) can be made up of piston main body (3-1), filter plate (3-2) and filter screen (3-3); Piston main body (3-1) is a porous plate, convex annular projection (3-1-3) is arranged on the both ends of the surface of plate, form annular ditch (3-1-2) between annular projection, (3-1-1) in even distribution hole arranged in the ditch, annular projection is cut off by radially-arranged groove (3-1-4), makes between the annular ditch (3-1-2) to communicate.The effect of piston main body is for piston provides enough compressions, keeps the required physical strength of post bed pressure, and moving phase is as far as possible evenly distributed; The effect of piston filter plate is the even distribution of guaranteeing moving phase; The effect of piston sample side screen is to stop the solids such as carrier in the sample to enter the post bed, and the filter screen of post bed side is to prevent that the filler in the post bed from falling to overflow.
The present invention has in the chromatographic column of axial compression fastener, and the sealing surface of described tubular bar (2) can be the plane, also can be the inclined-plane.When adopting trapezoidal O-ring seal, bevel angle is 27～30 °; When adopting " O " RunddichtringO, bevel angle is 35～45 °.
Adopt the such structure of the present invention, during the dress post, screw the fastening bolt of connection chromatographic column flange and compression flange, machine power is compressed the post bed, fix by compression flange tubular bar and piston, and can overcome the space that produces in the elution process.The axial compression fastener of this utility model also available hydraulic device screws fastening bolt again and reaches the fixed leg bed, and remaining certain pressure in elution process earlier with after the compacting of post bed.Space in the tubular bar can be used as the sample chamber of solid sample, uses the tubular bar of different length, can satisfy the demand of different sample sizes, and when chromatographic column regeneration was used, it was convenient to change sample.In a word, the present invention overcome the powered compressor fastener bulky, cost an arm and a leg and the limited defective of power that screw turncap compression set provides, for the chromatographic column compression provides simple, effective, convenient and economic technique device, can be widely used in semi-preparative column, preparative column and the commercial production chromatographic column of all size.
Description of drawings:
Fig. 1 is the cut-open view of first embodiment of axial compression fastener of the present invention;
Fig. 2 is that another embodiment compression flange of axial compression fastener of the present invention and tubular bar are the cut-open view of one;
Fig. 3 is the cut-open view of axial compression fastener piston main body of the present invention;
Fig. 4 is the vertical view of axial compression fastener piston main body end face of the present invention;
Fig. 5 is the cut-open view of another embodiment of axial compression fastener of the present invention.
Embodiment:
Axial compression fastener shown in Figure 1, be made up of compression flange 1, tubular bar 2, piston 3, O-ring seal 4 and fastening bolt 5: compression flange 1 has location and the hermetically-sealed construction with chromatographic column end cap 6, chromatographic column flange 7 and tubular bar 2 couplings; Tubular bar 2 outer walls are close to the chromatographic column inwall, and can move in post, and tubular bar two ends are by O-ring seal 4 and compression flange 1, chromatographic column inwall and piston 3 sealings, and the sealing surface of tubular bar 2 is the inclined-plane, and bevel angle is 45 °, and O-ring seal is " O " RunddichtringO; Space 8 in the tubular bar 2 can be used as the sample chamber; Piston 3 is made up of piston main body 3-1, filter plate 3-2 and filter screen 3-3; Piston main body 3-1 is the porous plate that adequate thickness is arranged, convex annular projection 3-1-3 is arranged on the both ends of the surface of plate, form annular ditch 3-1-2 between annular projection, even distribution hole 3-1-1 is arranged in the ditch, annular projection is cut off by radially-arranged groove 3-1-4, makes between the annular ditch 3-1-2 to communicate.
During the dress post, screw connection chromatographic column flange 7 and the fastening bolt 5 that compresses flange 1, machine power is compressed the post bed by compression flange 1, tubular bar 2 and piston 3 and is fixing.
After installing post, with the solid sample sample chamber 8 of entirely packing into, sample surfaces covers a filter screen, pours silica sand or coarse particle filler at filter screen again, install the styletable lid after, chromatographic column can be carried out chromatography.
During chromatography, moving phase through the sample chamber 8, pass piston upper screen 3-3, piston main body hole 3-1-1, be distributed to equably on the piston lower screen 3-3 by piston filter plate 3-2 and enter the post bed.
Chromatographic column of the present invention also can adopt other version, as tubular bar 2 and compression flange 1 are machined directly to a whole 1-1, or tubular bar 2, compression flange 1 and piston main body hole 3-1 is machined directly to an integral body; The sealing surface of tubular bar (2) can be the plane, also can be the inclined-plane; When adopting trapezoidal O-ring seal, bevel angle is 27～30 °; Adopt " O " during RunddichtringO, bevel angle is 35～45 °.
Chromatographic column of the present invention also can adopt other pressure source, as using hydraulic means 9 earlier with after the compacting of post bed, screws fastening bolt 5 fixed leg beds again, and remaining certain pressure in elution process.
Claims (7)
1, a kind of chromatographic column that has the axial compression fastener, chromatographic column end cap (6) by chromatogram void column (7) and two ends constitutes, it is characterized in that: increase by an axial compression fastener, form by compression flange (1), tubular bar (2), piston (3) and fastening bolt (5); Described compression flange (1) has location and the hermetically-sealed construction with chromatogram void column (7) flange coupling; Described tubular bar (2) is close to the tubular structure of chromatographic column inwall for the outer wall that slides in post; The porous structure of described piston (3) in post, sliding; The chromatogram void column is inserted filler in (7), filler top is followed successively by piston (3), tubular bar (2), compression flange (1), chromatographic column end cap (6), and fastening bolt (5) is fastenedly connected chromatographic column in aggregates with the flange of the flange of chromatogram void column (7), compression flange (1), chromatographic column end cap (6).
2, according to the described chromatographic column that has the axial compression fastener of claim 1, its feature is equipped with hydraulic means (9) between described compression flange (1) and chromatographic column end cap (6).
3, according to the described chromatographic column that has the axial compression fastener of claim 1, it is characterized in that: described tubular bar two ends are by O-ring seal (4) and compression flange (1), chromatographic column inwall and piston (3) sealing.
4, according to the described chromatographic column that has the axial compression fastener of claim 1, it is characterized in that: described tubular bar (2) and compression flange (1) as a whole (1-1).
5, according to the described chromatographic column that has the axial compression fastener of claim 1, it is characterized in that: described piston (3) is made up of piston main body (3-1), filter plate (3-2) and filter screen (3-3); Piston main body (3-1) is a porous plate, convex annular projection (3-1-3) is arranged on the both ends of the surface of plate, form annular ditch (3-1-2) between annular projection, (3-1-1) in even distribution hole arranged in the ditch, annular projection is cut off by radially-arranged groove (3-1-4), makes between the annular ditch (3-1-2) to communicate.
6, according to claim 1 or the 3 described chromatographic columns that have the axial compression fastener, it is characterized in that: the sealing surface of described tubular bar (2) is the plane, or the inclined-plane.
7, according to the described chromatographic column that has the axial compression fastener of claim 6, it is characterized in that: when adopting trapezoidal O-ring seal, bevel angle is 27～30 °; When adopting " O " RunddichtringO, bevel angle is 35～45 °.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03133507 CN1230236C (en) | 2003-05-28 | 2003-05-28 | Chromatographic column with axial compression fastener |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03133507 CN1230236C (en) | 2003-05-28 | 2003-05-28 | Chromatographic column with axial compression fastener |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1552501A true CN1552501A (en) | 2004-12-08 |
| CN1230236C CN1230236C (en) | 2005-12-07 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 03133507 Expired - Fee Related CN1230236C (en) | 2003-05-28 | 2003-05-28 | Chromatographic column with axial compression fastener |
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| Country | Link |
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| CN (1) | CN1230236C (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011069289A1 (en) * | 2009-12-10 | 2011-06-16 | 利穗科技(苏州)有限公司 | Visible fluorescent chromatography column |
| CN102648498A (en) * | 2009-10-08 | 2012-08-22 | 通用电气健康护理有限公司 | Chromatography components |
| CN110327661A (en) * | 2013-09-27 | 2019-10-15 | 通用电气健康护理生物科学股份公司 | Chromatographic column component |
-
2003
- 2003-05-28 CN CN 03133507 patent/CN1230236C/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102648498A (en) * | 2009-10-08 | 2012-08-22 | 通用电气健康护理有限公司 | Chromatography components |
| WO2011069289A1 (en) * | 2009-12-10 | 2011-06-16 | 利穗科技(苏州)有限公司 | Visible fluorescent chromatography column |
| CN110327661A (en) * | 2013-09-27 | 2019-10-15 | 通用电气健康护理生物科学股份公司 | Chromatographic column component |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1230236C (en) | 2005-12-07 |
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