CN203043621U - Liquid chromatographic column - Google Patents
Liquid chromatographic column Download PDFInfo
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- CN203043621U CN203043621U CN 201320062396 CN201320062396U CN203043621U CN 203043621 U CN203043621 U CN 203043621U CN 201320062396 CN201320062396 CN 201320062396 CN 201320062396 U CN201320062396 U CN 201320062396U CN 203043621 U CN203043621 U CN 203043621U
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- chromatographic column
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Abstract
The utility model discloses a liquid chromatographic column. A liquid inlet tube of a high-pressure mobile phase is arranged on the top of an upper cavity of a chromatographic column tube; the high-pressure mobile phase enters the upper cavity of the chromatographic column tube to push and press a piston body to move downward; the input end for mixed homogenate of a fixed phase and a mobile phase compressed below the piston is arranged on the upper cavity and is provided with an input valve; a separated material passes through the high-pressure liquid inlet tube and an upper screen plate and a distributor to be, is uniformly shunted, then enters a lower cavity, and is adsorbed and separated in the fixed phase; and a column bottom screen plate, the distributor and a liquid outlet are arranged at the bottom of the lower cavity. The liquid chromatographic column can solve the technical problems of equipment pollution, influence on the quality of biochemical liquid and the like caused by large size and hydraulic oil leakage in a conventional device in which a high-pressure hydraulic oil cylinder pushes a piston body to compress a fixed phase and a mobile phase.
Description
Technical field
The utility model relates to a kind of liquid-phase chromatographic column, relates in particular to a kind ofly to need not that the high-pressure and hydraulic oil cylinder promotes piston body and the method and the liquid-phase chromatographic column that reach the compressed mixed homogenate.
Background technology
The liquid-phase chromatographic column of prior art is when separation, purified mixture, the piston body that at first needs the axis of the piston of high-pressure and hydraulic oil cylinder to promote in the chromatographic column is descending, the fixing phase of compression and mobile mixing homogenate mutually, and the major part in the homogenate flowed sieve plate pushes away at the bottom of the post, reach and fix mutually again in order, arrange closely, different separated materials be can adsorb successively, thereby separation, purified mixture implemented.Concrete structure is referring to Fig. 1, its operation principle is as follows: high-pressure and hydraulic oil cylinder (1), its the axis of the piston (2) promotes the piston body (5) of chromatographic column, the fixedly phase that compression enters from transfer valve (12) with mobile mutually the mixing homogenate and the major part the homogenate flowed pass through sieve plate and distributor (7) at the bottom of the post mutually, outlet (8) flows into collection container, reach again orderly through the mixing homogenate that takes off liquid, closely be arranged as fixedly phase (6) and pressurize, compact arranged fixedly phase can be adsorbed different separated materials successively, thereby implement to separate, the purpose of the separated material of purifying, separated material takes high-pressure delivery to enter from feed tube (3), again by upper sieve plate and distributor (4), filter the impurity in the separated material, and evenly the shunting back fixedly is being adsorbed in the phase (6), enter from feed tube (3) through high-pressure flow again and release the separated material that is adsorbed the fixing phase (6), reach sieve plate (7) down from distributor according to this, by drain pipe (8) inflow collection container separately, namely finished the purification of a batch, purifying process, if will carry out next purification in batches, purifying is produced, high pressure is imported separated material again, again with high-pressure flow with it
Release, collect gatherer separately, move in circles, distributor reaches down, and sieve plate (7) also is used for hindering fixedly phase particulate simultaneously, keeping fixing is not run off mutually, the post bucket (9) of chromatographic column and airtight connection of chromatographic column lower flange (10), unloading lower flange can will use the high pressure liquid oil pressuring cylinder piston that fixedly communicated for a long time to promote to push away under the piston body continuation with the fixing post bucket of releasing mutually, clean in order to reuse, it is not airtight with the post bucket that chromatographic column does not have airtight upper flange (11), be mainly used in support and the connection of high-pressure and hydraulic oil cylinder, simultaneously feed tube (3) also in the upper flange by entering piston body.But implement this method and not only need a bulky high-pressure and hydraulic oil cylinder be set in the chromatographic column upper end, cause the equipment hypervelocity, be difficult to install in common biochemical workshop, and high-pressure and hydraulic oil cylinder and fluid pressure line thereof have the hydraulic oil seepage for a long time unavoidably, cause equipment pollution, influence the quality of biochemical soup.
With reference to Fig. 2, flowing and fixing the homogenate that mixes mutually enters the chromatographic column cavity of resorption but not compressed state also from transfer valve (12).
Summary of the invention
The purpose of this utility model provides a kind of liquid-phase chromatographic column, promote bulky, the hydraulic oil seepage that the mutually fixing and mobile device mutually of piston body compression exists to solve traditional high-pressure and hydraulic oil cylinder, cause equipment pollution, influence the technical problems such as quality of biochemical soup.
In order to realize the foregoing invention purpose, the technical scheme that the utility model adopts is as follows:
A kind of liquid-phase chromatographic column, top of chamber on gc column tube, be provided with the liquid-in pipe of high-pressure flow phase, it is descending that described high-pressure flow enters the epicoele pushing piston body of gc column tube mutually, and the input of the described piston compressed fixedly phase in below and mobile mixing homogenate mutually is located at epicoele and transfer valve is arranged; Separated material evenly enters cavity of resorption after the shunting by high pressure feed tube, upper sieve plate and distributor, is adsorbed in mutually and separates fixing; Sieve plate, distributor and liquid outlet at the bottom of the cavity of resorption bottom is provided with post.
A kind of liquid-phase chromatographic column, chromatographic column is divided into epicoele and cavity of resorption by chromatographic column upper flange, chromatographic column lower flange and the piston that is positioned at the chromatographic column inner chamber with it, the guide rod hermetically passing upper flange of described piston is fixed on the back timber, the back timber two ends are connected with the lowering or hoisting gear that is installed in the chromatographic column both sides respectively, and this lowering or hoisting gear is floating type in off working state; High-pressure flow enters into the epicoele of chromatographic column by liquid-in pipe, and the pushing piston body is descending; The input of the piston compressed fixedly phase in below and mobile mixing homogenate mutually is located at epicoele and transfer valve is arranged, and separated material enters cavity of resorption by the feed tube that is arranged on center of pilot, piston body sieve plate and the distributor of piston bottom surface; Described lower flange is provided with drain pipe, and its inwall is provided with distributor and reaches sieve plate down.
The lowering or hoisting gear that described back timber two ends connect is a kind of in low-pressure hydraulic oil cylinder lift drive mechanism, the lift drive mechanism that adopts ball screw arrangement or the screw thread pair lift drive mechanism.
The beneficial effects of the utility model are: reduced manufacturing cost, avoided the equipment superelevation again, be fit to the installation in common biochemical workshop, also avoided the quality of the seepage assurance soup of hydraulic oil simultaneously.
Description of drawings
Fig. 1 is the structural representation of existing liquid-phase chromatographic column.
Fig. 2 is that the mobile of existing liquid-phase chromatographic column enters the not structural representation of compressed state of chromatographic column cavity of resorption with the fixing homogenate that mixes mutually from transfer valve.
Fig. 3 is liquid-phase chromatographic column example structure schematic diagram described in the utility model.
Fig. 4 is another example structure schematic diagram of liquid-phase chromatographic column described in the utility model.
The specific embodiment
Describe principle of the present utility model in detail below in conjunction with accompanying drawing:
With reference to Fig. 3, be that the utility model adopts that the mobile phase pushing piston body of self technology is descending to compress mutually fixing and mobile mixing homogenate mutually, its method and install as follows:
The high-pressure flow phase (13) of the utility model employing self technology is descending by the epicoele pushing piston body that high-pressure flow phase liquid-in pipe (18) enters into chromatographic column, the fixedly phase of compression cavity of resorption with mobile mutually the mixing homogenate and the major part in the homogenate flowed pass through sieve plate and distributor (7) at the bottom of the post mutually, outlet (8) flows into collection container, reach again orderly, closely arrange the fixedly method of phase (6), when flowing phase pushing piston body, its epicoele has very big interior pressure, therefore upper flange (14) is except needs and chromatographic column seal, also need guide rod (15) sealing with piston body (5) simultaneously, can accomplish that just high-pressure flow phase (13) is not by earial drainage, feed tube this moment (3) also changes its course and enters from guide rod (15) center, separated material is by feed tube (3), piston body sieve plate and distributor (4), fixedly be adsorbed in the phase (6), enter from feed tube (3) through high-pressure flow again and release the separated material that is adsorbed the fixing phase (6), finished the purification of a batch, purifying process, low-pressure hydraulic oil cylinder (16) is installed the both sides of chromatographic column in addition, can not cause equipment pollution to chromatographic column, connect with back timber (17), its effect only is to shift the chromatographic column top on the piston onto, can pack into from transfer valve (12) and fix mutually and mobile mixing homogenate mutually, perhaps onto the chromatographic column bottom will be shifted under the piston, release column jecket, be convenient to clean fixedly phase, in order to reuse, flange should be unloaded down from chromatographic column earlier at the bottom of the post at this moment, in addition when low-pressure hydraulic oil cylinder during in off working state, its piston should be in quick condition, avoids when piston body (5), cause the phenomenon of low-pressure hydraulic oil cylinder piston interference when pushing away under the back timber (17).
With reference to Fig. 4, the utility model also can adopt ball-screw (19) structure, substitutes the low-pressure hydraulic oil cylinder, the slowspeed machine that drives ball-screw also should arrange the measure of the scheme of floating, when upper and lower displacement produces the ball-screw rotation, also can drive the slowspeed machine rotation, reach no interference.
Ball-screw of the present utility model equally also can adopt screw thread pair to substitute, and the method for taking nut to separate, connect with nut seat is avoided interference.
Claims (3)
1. liquid-phase chromatographic column, it is characterized in that, top of chamber on gc column tube, be provided with the liquid-in pipe of high-pressure flow phase, it is descending that described high-pressure flow enters the epicoele pushing piston body of gc column tube mutually, and the input of the described piston compressed fixedly phase in below and mobile mixing homogenate mutually is located at epicoele and transfer valve is arranged; Separated material evenly enters cavity of resorption after the shunting by high pressure feed tube, upper sieve plate and distributor, is adsorbed in mutually and separates fixing; Sieve plate, distributor and liquid outlet at the bottom of the cavity of resorption bottom is provided with post.
2. a kind of liquid-phase chromatographic column according to claim 1, it is characterized in that, chromatographic column is divided into epicoele and cavity of resorption by chromatographic column upper flange, chromatographic column lower flange and the piston that is positioned at the chromatographic column inner chamber with it, the guide rod hermetically passing upper flange of described piston is fixed on the back timber, the back timber two ends are connected with the lowering or hoisting gear that is installed in the chromatographic column both sides respectively, and this lowering or hoisting gear is floating type in off working state; High-pressure flow enters into the epicoele of chromatographic column by liquid-in pipe, and the pushing piston body is descending; The input of the piston compressed fixedly phase in below and mobile mixing homogenate mutually is located at epicoele and transfer valve is arranged, and separated material enters cavity of resorption by the feed tube that is arranged on center of pilot, piston body sieve plate and the distributor of piston bottom surface; Described lower flange is provided with drain pipe, and its inwall is provided with distributor and reaches sieve plate down.
3. a kind of liquid-phase chromatographic column according to claim 2, it is characterized in that the lowering or hoisting gear that described back timber two ends connect is a kind of in low-pressure hydraulic oil cylinder lift drive mechanism, the lift drive mechanism that adopts ball screw arrangement or the screw thread pair lift drive mechanism.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320062396 CN203043621U (en) | 2013-02-04 | 2013-02-04 | Liquid chromatographic column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320062396 CN203043621U (en) | 2013-02-04 | 2013-02-04 | Liquid chromatographic column |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203043621U true CN203043621U (en) | 2013-07-10 |
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ID=48726622
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201320062396 Expired - Lifetime CN203043621U (en) | 2013-02-04 | 2013-02-04 | Liquid chromatographic column |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203043621U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103111092A (en) * | 2013-02-04 | 2013-05-22 | 北京创新通恒科技有限公司 | Method for compression of evenly-mixed slurry liquid for liquid chromatography and liquid-phase chromatography column |
| CN103752039A (en) * | 2014-01-07 | 2014-04-30 | 南京诺云生物科技有限公司 | Self-supporting modular chromatographic column and application thereof |
| CN104307207A (en) * | 2013-12-17 | 2015-01-28 | 长沙市源创精科生物技术有限公司 | A fully-automatic chromatographic column |
| CN104801066A (en) * | 2015-04-07 | 2015-07-29 | 利穗科技(苏州)有限公司 | Chromatographic column with maintenance mechanism |
-
2013
- 2013-02-04 CN CN 201320062396 patent/CN203043621U/en not_active Expired - Lifetime
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103111092A (en) * | 2013-02-04 | 2013-05-22 | 北京创新通恒科技有限公司 | Method for compression of evenly-mixed slurry liquid for liquid chromatography and liquid-phase chromatography column |
| CN104307207A (en) * | 2013-12-17 | 2015-01-28 | 长沙市源创精科生物技术有限公司 | A fully-automatic chromatographic column |
| CN103752039A (en) * | 2014-01-07 | 2014-04-30 | 南京诺云生物科技有限公司 | Self-supporting modular chromatographic column and application thereof |
| CN103752039B (en) * | 2014-01-07 | 2015-09-30 | 南京诺云生物科技有限公司 | Self-supporting modularity chromatographic column and uses thereof |
| CN104801066A (en) * | 2015-04-07 | 2015-07-29 | 利穗科技(苏州)有限公司 | Chromatographic column with maintenance mechanism |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20130710 |
|
| CX01 | Expiry of patent term |