CN103111092B - Method for compression of evenly-mixed slurry liquid for liquid chromatography and liquid-phase chromatography column - Google Patents
Method for compression of evenly-mixed slurry liquid for liquid chromatography and liquid-phase chromatography column Download PDFInfo
- Publication number
- CN103111092B CN103111092B CN201310042974.1A CN201310042974A CN103111092B CN 103111092 B CN103111092 B CN 103111092B CN 201310042974 A CN201310042974 A CN 201310042974A CN 103111092 B CN103111092 B CN 103111092B
- Authority
- CN
- China
- Prior art keywords
- liquid
- phase
- chromatographic column
- distributor
- pressure
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000007788 liquid Substances 0.000 title claims abstract description 53
- 238000000034 method Methods 0.000 title claims abstract description 19
- 239000007791 liquid phase Substances 0.000 title claims abstract description 14
- 230000006835 compression Effects 0.000 title claims abstract description 7
- 238000007906 compression Methods 0.000 title claims abstract description 7
- 238000004587 chromatography analysis Methods 0.000 title abstract 4
- 239000011268 mixed slurry Substances 0.000 title abstract 3
- 238000004811 liquid chromatography Methods 0.000 title abstract 2
- 239000012071 phase Substances 0.000 claims abstract description 32
- 239000000463 material Substances 0.000 claims abstract description 21
- 239000010720 hydraulic oil Substances 0.000 claims abstract description 18
- 238000000746 purification Methods 0.000 claims description 5
- 238000003491 array Methods 0.000 claims description 4
- 238000005516 engineering process Methods 0.000 claims description 3
- 239000012535 impurity Substances 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 3
- 238000007873 sieving Methods 0.000 abstract 2
- 239000003814 drug Substances 0.000 abstract 1
- 238000010586 diagram Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
Abstract
The invention discloses a method for the compression of an evenly-mixed slurry liquid for liquid chromatography and a liquid-phase chromatography column. A liquid inlet pipe of a high-pressure flowing phase is arranged at the top part of an upper cavity of a chromatography column pipe; the high-pressure flowing phase enters the upper cavity of the chromatography column pipe to push and press a piston body to move downwards; the input end of the mixed slurry liquid of a fixed phase and a flowing phase which are compressed below the piston is arranged at the upper cavity and provided with an input valve; after the flow direction of a separated material is evenly separated through the high-pressure liquid inlet pipe, an upper sieving plate and a distributor, the separated material flows into a lower cavity to be adsorbed and separated in the fixed phase; and a column bottom sieving plate, the distributor and a liquid outlet are arranged at the bottom of the lower cavity. According to the method, the technical problems of the traditional device utilizing a high-pressure hydraulic cylinder to drive the piston to compress the fixed phase and the flowing phase that the volume is bulky, the equipment is polluted due to the leakage of hydraulic oil, and the quality of a biochemical medicine liquid is influenced and the like are solved.
Description
Technical field
The present invention relates to a kind of liquid-phase chromatographic column, particularly relate to a kind of method and the liquid-phase chromatographic column that reach compressed mixed homogenate without the need to high-pressure and hydraulic oil cylinder pushing piston body.
Background technology
The liquid-phase chromatographic column of prior art is when separation, purified mixture, first the piston body needing the piston axle of high-pressure and hydraulic oil cylinder to promote in chromatographic column is descending, the mixing homogenate of compression Stationary liquid and mobile phase, and the most of mobile phase in homogenate is gone out from sieve plate press at the bottom of post, reach Stationary liquid again to arrange in order, closely, different separated materials can be adsorbed successively, thus implement separation, purified mixture.Concrete structure is see Fig. 1, its operation principle is as follows: high-pressure and hydraulic oil cylinder (1), its piston axle (2) promotes the piston body (5) of chromatographic column, compress Stationary liquid and the mobile phase entered from transfer valve (12) mixing homogenate and by the most of mobile phase homogenate sieve plate and distributor (7) at the bottom of post, outlet (8) flows into collection container, mixing homogenate through de-liquid reaches again orderly, close-packed arrays becomes Stationary liquid (6) and pressurize, compact arranged Stationary liquid can adsorb different separated materials successively, thus implement to be separated, the object of the separated material of purifying, separated material takes high-pressure delivery to enter from feed tube (3), again by upper sieve plate and distributor (4), filter the impurity in separated material, and adsorbed in Stationary liquid (6) after uniform divided flows, enter release Stationary liquid (6) by the separated material adsorbed from feed tube (3) through high-pressure flow again, according to this from distributor and lower sieve plate (7), respective collection container is flowed into by drain pipe (8), namely the purification of a batch is completed, purifying process, to carry out the purification of next batch, purifying is produced, the separated material of high input voltage again, again by high-pressure flow by it
Release, collect respective collector, move in circles, distributor and lower sieve plate (7) are also simultaneously for hindering Stationary liquid particulate, Stationary liquid is kept not run off, the post bucket (9) of chromatographic column connects with chromatographic column lower flange (10) is airtight, unload lower flange the Stationary liquid used for a long time to be promoted to push away under piston body continues Stationary liquid is released post bucket by high-pressure and hydraulic oil cylinder piston, carry out cleaning to reuse, chromatographic column is not airtight without airtight upper flange (11) and post bucket, be mainly used in support and the connection of high-pressure and hydraulic oil cylinder, simultaneously feed tube (3) also in upper flange by entering piston body.But implement this method not only to need to hold on a column and arrange a bulky high-pressure and hydraulic oil cylinder, cause equipment hypervelocity, be difficult to install in common biochemical workshop, and high-pressure and hydraulic oil cylinder and fluid pressure line thereof have hydraulic oil seepage for a long time unavoidably, cause equipment pollution, affect the quality of biochemical liquid.
With reference to Fig. 2, the homogenate that mobile phase mixes with Stationary liquid enters chromatographic column cavity of resorption but also un-compressed state from transfer valve (12).
Summary of the invention
The object of this invention is to provide a kind of method and liquid-phase chromatographic column of compressed mixed homogenate of liquid chromatogram, to solve traditional high-pressure and hydraulic oil cylinder pushing piston body compression Stationary liquid and bulky, the hydraulic oil seepage of mobile phase device existence, cause equipment pollution, affect the technical problems such as the quality of biochemical liquid.
In order to realize foregoing invention object, the technical solution adopted in the present invention is as follows:
A kind of method of compressed mixed homogenate of liquid chromatogram, wherein compressing Stationary liquid and the step of the mixing homogenate of mobile phase, to be that the high-pressure flow adopting self production technology to configure enters the epicoele pushing piston body of gc column tube mutually descending, compression is implemented to the mixing homogenate entering cavity of resorption, reaches again in order, close-packed arrays becomes Stationary liquid and pressurize; Separated material takes high-pressure delivery to enter from feed tube, by upper sieve plate and distributor, filter the impurity in separated material, and adsorbed in Stationary liquid after uniform divided flows, enter release Stationary liquid by the separated material adsorbed from feed tube through high-pressure flow again, according to this from distributor and lower sieve plate, flow into respective collection container by drain pipe, namely complete the purification of a batch, purifying process.
A kind of liquid-phase chromatographic column, at the upper top of chamber of gc column tube, be provided with the liquid-in pipe of high-pressure flow phase, the epicoele pushing piston body that described high-pressure flow enters gc column tube is mutually descending, is located at epicoele and has transfer valve below described piston by the input of the mixing homogenate of the Stationary liquid that compresses and mobile phase; Separated material, by entering cavity of resorption after high pressure feed tube, upper sieve plate and distributor uniform divided flows, is adsorbed and is separated in Stationary liquid; Sieve plate at the bottom of post, distributor and liquid outlet is provided with bottom cavity of resorption.
A kind of liquid-phase chromatographic column, chromatographic column is divided into epicoele and cavity of resorption by chromatographic column upper flange, chromatographic column lower flange and the piston that is positioned at chromatographic column inner chamber, the guide rod hermetically passing upper flange of described piston is fixed on back timber, back timber two ends are connected with the lowering or hoisting gear being arranged on chromatographic column both sides respectively, and this lowering or hoisting gear is floating type in off working state; High-pressure flow enters into the epicoele of chromatographic column by liquid-in pipe, and pushing piston body is descending; Be located at epicoele by the input of the mixing homogenate of the Stationary liquid that compresses and mobile phase and have transfer valve below piston, separated material enters cavity of resorption by being arranged on the feed tube of center of pilot, the piston body sieve plate of piston bottom surface and distributor; Described lower flange is provided with drain pipe, and its inwall is provided with distributor and lower sieve plate.
The lowering or hoisting gear that described back timber two ends connect is the one in low-pressure hydraulic oil cylinder lift drive mechanism, the lift drive mechanism adopting ball screw arrangement or screw thread pair lift drive mechanism.
The invention has the beneficial effects as follows: reduce manufacturing cost, turn avoid equipment superelevation, be applicable to the installation in common biochemical workshop, the seepage that simultaneously it also avoid hydraulic oil ensures the quality of liquid.
Accompanying drawing explanation
Fig. 1 is the structural representation of existing liquid-phase chromatographic column.
Fig. 2 is homogenate that the mobile phase of existing liquid-phase chromatographic column mixes with Stationary liquid enters the un-compressed state of chromatographic column cavity of resorption structural representation from transfer valve.
Fig. 3 is liquid-phase chromatographic column example structure schematic diagram of the present invention.
Fig. 4 is another example structure schematic diagram of liquid-phase chromatographic column of the present invention.
Detailed description of the invention
Principle of the present invention is described in detail below in conjunction with accompanying drawing:
With reference to Fig. 3, for the present invention adopts the descending mixing homogenate compressing Stationary liquid and mobile phase of the mobile phase pushing piston body of self technique, its method and device as follows:
The epicoele pushing piston body that the present invention adopts the high-pressure flow phase (13) of self technique to enter into chromatographic column by high-pressure flow phase liquid-in pipe (18) is descending, the compression Stationary liquid of cavity of resorption and the mixing homogenate of mobile phase by the most of mobile phase in homogenate sieve plate and distributor (7) at the bottom of post, outlet (8) flows into collection container, reach again orderly, the method of close-packed arrays Stationary liquid (6), during mobile phase pushing piston body, its epicoele has very large interior pressure, therefore upper flange (14) is except needs and chromatographic column seal, also need to seal with the guide rod of piston body (5) (15) simultaneously, just can accomplish that high-pressure flow phase (13) is not by earial drainage, now feed tube (3) also changes its course from guide rod (15) center and enters, separated material is by feed tube (3), piston body sieve plate and distributor (4), adsorbed in Stationary liquid (6), enter release Stationary liquid (6) by the separated material adsorbed from feed tube (3) through high-pressure flow again, complete the purification of a batch, purifying process, the both sides of chromatographic column installed by low-pressure hydraulic oil cylinder (16) in addition, equipment pollution can not be caused to chromatographic column, connect with back timber (17), its effect is only used to piston to be shifted chromatographic column top onto, the mixing homogenate of Stationary liquid and mobile phase can be loaded from transfer valve (12), or shift column bottom onto by under piston, release column jecket, be convenient to clean Stationary liquid, to reuse, now flange at the bottom of post should first be unloaded down from chromatographic column, in addition when low-pressure hydraulic oil cylinder is in off working state, its piston should be in quick condition, avoid when piston body (5), the phenomenon of low-pressure hydraulic oil cylinder piston interference is caused when pushing away under back timber (17).
With reference to Fig. 4, the present invention also can adopt ball-screw (19) structure, substitutes low-pressure hydraulic oil cylinder, drive the slowspeed machine of ball-screw that the measure of floating solution also should be set, when upper and lower displacement produces ball-screw rotation, slowspeed machine also can be driven to rotate, reach without interference.
Ball-screw of the present invention equally also can adopt screw thread pair to substitute, and the method take nut to be separated with nut seat, connecting, avoids interference.
Claims (2)
1. the method for the compressed mixed homogenate of a liquid chromatogram, it is characterized in that: described liquid-phase chromatographic column, at the upper top of chamber of gc column tube, be provided with the feed tube of high-pressure flow phase, the epicoele pushing piston body that described high-pressure flow enters gc column tube is mutually descending, is located at epicoele and has transfer valve below described piston by the input of the mixing homogenate of the Stationary liquid that compresses and mobile phase; Separated material, by entering cavity of resorption after high pressure feed tube, upper sieve plate and distributor uniform divided flows, is adsorbed and is separated in Stationary liquid; Lower sieve plate, distributor and liquid outlet is provided with bottom cavity of resorption;
Described chromatographic column is divided into epicoele and cavity of resorption by chromatographic column upper flange, chromatographic column lower flange and the piston that is positioned at chromatographic column inner chamber, the guide rod hermetically passing upper flange of described piston is fixed on back timber, back timber two ends are connected with the lowering or hoisting gear being arranged on chromatographic column both sides respectively, and this lowering or hoisting gear is floating type in off working state; High-pressure flow enters into the epicoele of chromatographic column by feed tube, and pushing piston body is descending; Be located at epicoele by the input of the mixing homogenate of the Stationary liquid that compresses and mobile phase and have transfer valve below piston, separated material enters cavity of resorption by being arranged on the feed tube of center of pilot, the upper sieve plate of piston bottom surface and distributor; Described lower flange is provided with drain pipe, and its inwall is provided with distributor and lower sieve plate;
Wherein compressing Stationary liquid and the step of the mixing homogenate of mobile phase, to be that the high-pressure flow adopting self production technology to configure enters the epicoele pushing piston body of gc column tube mutually descending, compression is implemented to the mixing homogenate entering cavity of resorption, reaches again in order, close-packed arrays becomes Stationary liquid and pressurize; Separated material takes high-pressure delivery to enter from feed tube, by upper sieve plate and distributor, filter the impurity in separated material, and adsorbed in Stationary liquid after uniform divided flows, high-pressure flow phase again through entering from feed tube, to release by the separated material adsorbed in Stationary liquid, according to this from distributor and lower sieve plate, flow into respective collection container by drain pipe, namely complete the purification of a batch, purifying process.
2. the method for the compressed mixed homogenate of a kind of liquid chromatogram according to claim 1, it is characterized in that, the lowering or hoisting gear that described back timber two ends connect is the one in low-pressure hydraulic oil cylinder lift drive mechanism, the lift drive mechanism adopting ball screw arrangement or screw thread pair lift drive mechanism.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310042974.1A CN103111092B (en) | 2013-02-04 | 2013-02-04 | Method for compression of evenly-mixed slurry liquid for liquid chromatography and liquid-phase chromatography column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310042974.1A CN103111092B (en) | 2013-02-04 | 2013-02-04 | Method for compression of evenly-mixed slurry liquid for liquid chromatography and liquid-phase chromatography column |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103111092A CN103111092A (en) | 2013-05-22 |
| CN103111092B true CN103111092B (en) | 2015-03-18 |
Family
ID=48409499
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310042974.1A Active CN103111092B (en) | 2013-02-04 | 2013-02-04 | Method for compression of evenly-mixed slurry liquid for liquid chromatography and liquid-phase chromatography column |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103111092B (en) |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3021366A1 (en) * | 1980-06-06 | 1981-12-17 | Merck Patent Gmbh, 6100 Darmstadt | SEPARATION COLUMN FOR THE LIQUID CHROMATOR GRAPHI |
| US4597866A (en) * | 1983-12-02 | 1986-07-01 | Groupe Industriel De Realisations Et Applications | Chromatography apparatus |
| JP2595015B2 (en) * | 1988-02-25 | 1997-03-26 | 東ソー株式会社 | Method for forming a packed bed of liquid chromatography column, and movable stopper type column apparatus used in the method |
| CN202478652U (en) * | 2012-03-22 | 2012-10-10 | 利穗科技(苏州)有限公司 | Chromatographic column |
| CN102836572B (en) * | 2012-09-17 | 2014-11-05 | 於红军 | Double-cavity dynamic compression column for chromatographic separation |
| CN203043621U (en) * | 2013-02-04 | 2013-07-10 | 北京创新通恒科技有限公司 | Liquid chromatographic column |
-
2013
- 2013-02-04 CN CN201310042974.1A patent/CN103111092B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN103111092A (en) | 2013-05-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4927531A (en) | Axially adjustable-type column device | |
| CN101601942B (en) | Split shaking device of filter press filter board | |
| CN203043621U (en) | Liquid chromatographic column | |
| CN214344591U (en) | Hydraulic drive formula developments chromatography device | |
| CN103111092B (en) | Method for compression of evenly-mixed slurry liquid for liquid chromatography and liquid-phase chromatography column | |
| CN213703865U (en) | Hollow brick pressure forming device | |
| CN201859082U (en) | Piston-type clayey soil pore water mechanical squeezing instrument | |
| WO2012074464A1 (en) | Method for securing a distributor plate to a backing plate of a chromatography column and a chromatography column. | |
| JP4968489B2 (en) | Chromatographic packing device | |
| CN215208871U (en) | Desulfurization and denitrification sewage treatment device | |
| CN212640269U (en) | Full-automatic solid-liquid separation system suitable for sludge treatment | |
| CN104399282B (en) | A kind of manual chromatographic column | |
| JP2707780B2 (en) | Packing method of movable stopper column for liquid chromatography | |
| CN201470181U (en) | Vibrating mechanism of filter press | |
| CN219595921U (en) | Novel hydraulic dynamic axial compression liquid chromatography column | |
| CN220376548U (en) | Sludge concentration dewatering device | |
| CN214389083U (en) | Ceramic color glaze solvent oil impurity separation device | |
| CN214917014U (en) | Novel ceramic slurry deironing device | |
| CN219449296U (en) | Liquid feeding device of filling machine for cosmetics | |
| CN110339606B (en) | Horizontal single-bin diaphragm filter press | |
| CN111318074A (en) | Efficient filtering device and using method thereof | |
| CN204543643U (en) | Chromatography column apparatus | |
| CN114571773B (en) | Petroleum recovery device | |
| CN220597143U (en) | Reverse osmosis water purification device | |
| CN208839187U (en) | A kind of use for laboratory purpose ceramic-film filter |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A method for compressing mixed homogenate of liquid chromatography and liquid chromatography column Effective date of registration: 20210709 Granted publication date: 20150318 Pledgee: Haidian Beijing science and technology enterprise financing Company limited by guarantee Pledgor: BEIJING CHUANGXIN TONGHENG SCIENCE AND TECHNOLOGY Co.,Ltd. Registration number: Y2021990000596 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20220714 Granted publication date: 20150318 Pledgee: Haidian Beijing science and technology enterprise financing Company limited by guarantee Pledgor: BEIJING CHUANGXIN TONGHENG SCIENCE AND TECHNOLOGY Co.,Ltd. Registration number: Y2021990000596 |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A method for compressing mixed homogenates in liquid chromatography and a liquid chromatography column Effective date of registration: 20231129 Granted publication date: 20150318 Pledgee: Bank of Nanjing Limited by Share Ltd. Beijing branch Pledgor: BEIJING CHUANGXIN TONGHENG SCIENCE AND TECHNOLOGY Co.,Ltd. Registration number: Y2023110000497 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right |