CN202478652U

CN202478652U - Chromatographic column

Info

Publication number: CN202478652U
Application number: CN2012201100287U
Authority: CN
Inventors: 周胜; 邹学文
Original assignee: LISURE SCIENCE (SUZHOU) CO Ltd
Current assignee: LISURE SCIENCE (SUZHOU) CO Ltd
Priority date: 2012-03-22
Filing date: 2012-03-22
Publication date: 2012-10-10
Anticipated expiration: 2022-03-22

Abstract

The utility model discloses a chromatographic column which comprises a column tube, an upper flange device, a lower flange device and a piston device, wherein the piston device is arranged at the middle part of the column tube and carries out axial compression in the column tube, a spiral infusion tube is connected between the upper flange device and the piston device, the thicknesses of the tube walls of the two ends of a column tube body are greater than the thickness of the tube wall of the middle part of the column tube body, the two ends of the column tube are provided with an upper locked groove and a lower locked groove, an upper clamp lock and a lower clamp lock are respectively arranged in the upper locked groove and the lower locked groove, and the upper clamp lock and the lower clamp lock tightly press the upper flange device and the lower flange device at the two ends of the column tube. Through the way, the chromatographic column disclosed by the utility model can realize the improvement of the pressure resistance of chromatographic columns, and can achieve the industrial-level production demand; and the chromatographic column is low in manufacturing cost and easy to use.

Description

Chromatographic column

Technical field

The utility model relates to chromatographic separation and purification and extractive technique field, relates in particular to a kind of chromatographic column.

Background technology

Along with developing rapidly of industries such as pharmacy, biochemical industry, the preparative liquid chromatography isolation technics obtains development and application more and more widely, has become the important method of separation and purifying complex mixture, is particularly useful for the separation of natural products.For improving separating rate, then need improve operating pressure at present, increase applied sample amount simultaneously; Reach the technical grade requirement, traditional method is to use stainless steel or glass to do column jecket, and the pillar diameter is in case increase like this; Cost of manufacture can roll up, and chromatographic column can seem especially heavy.

In the prior art, classical column chromatography has been widely used in natural product chemistry, pharmaceutical chemistry, Food Chemistry, organic synthesis, the preparation property separation of difficult separator in the field such as refinement worker slightly.Along with development of science and technology, some researchs need once can preparation property separation larger amt difficult separator, even consider whether can maximize chromatographic isolation, make it to become process and unit in the commercial production.In the preparative chromatography of classics, the sample size that chromatographic column can be born be with in the post fixedly the amount of phase be directly proportional, in order to increase the fractional dose of chromatographic column; People often increase the consumption of fixing phase and reach the purpose that increases fractional dose by the method that increases column diameter in the research work, with the increase of the classical column diameter of work, and the employed fixing reason of starting from economy and equipment mutually in the separation; The monodisperse particles that can not use small grain size very is phase fixedly; And being to use the parting material with certain particle size scope of larger particles, this just makes in the filling process of chromatographic column, is difficult to occur the fixedly classification phenomenon of phase with avoiding; And the diameter of chromatographic column is big more; The classification phenomenon is just serious more, and chromatographic column just more difficult filling is even, and the chromatographic column effect of being filled is just low more.

The utility model content

The technical problem that the utility model solves provides a kind of chromatographic column, can improve the withstand voltage properties of chromatographic column, reaches technical grade production demand, and low cost of manufacture is easy and simple to handle.

The technical scheme that its technical problem of the utility model solution is taked is: a kind of chromatographic column; Comprise column jecket, upper flange device; Lower flange device and piston apparatus, said piston apparatus are installed in the column jecket middle part and in column jecket, carry out axial compression, are connected with a spiral woven hose between said upper flange device and the piston apparatus; The two ends pipe thickness of said column jecket body is greater than the thickness of middle part tube wall; Offer locked groove and following locked groove at the two ends of said column jecket, the said locked groove of going up is built-in with kayser and following kayser respectively with following locked groove, and the said kayser of going up is pressed in the column jecket two ends to upper flange device and lower flange device with following kayser.

In preferred embodiment of the utility model; Said upper flange device comprises pressurization mouth, inlet, pressure release exhaust outlet, first sealing ring and upper flange; Said pressurization mouth, inlet and pressure release exhaust outlet are arranged in the upper surface of upper flange, and said upper flange seals and be pressed on the port of column jecket through first sealing ring and last kayser.

In preferred embodiment of the utility model; Said lower flange device comprises filler import and export, liquid outlet, first sieve plate, cylinder manifold, second sealing ring and lower flange; Said filler import and export and liquid outlet are arranged in the lower surface of lower flange; Said cylinder manifold is installed in the upper end of lower flange; Said first sieve plate is installed in the upper end of cylinder manifold, and said lower flange is through second sealing ring and the sealing of play kayser and be pressed on the lower port of column jecket, and the exit of said filler import and export and liquid outlet is respectively arranged with two pass ball valves.

In preferred embodiment of the utility model; Said piston apparatus comprises the 3rd sealing ring, piston, flow distribution plate and second sieve plate; Said the 3rd sealing ring packed-piston and vial wall, said flow distribution plate is installed in the lower end of piston, and said second sieve plate is installed in the lower end of flow distribution plate.

In preferred embodiment of the utility model, said column jecket comprises liner and surrounding layer, and said liner is PP, PVDF or 316L stainless-steel thin-wall pipe, and said surrounding layer is for being substrate glass fiber winding process layer with the epoxy resin material.

In preferred embodiment of the utility model, said column jecket is that a column jecket independently uses or a plurality of parallelly connected column jecket uses simultaneously.

The beneficial effect of the utility model is: be used for preparative chromatography separation and purification and extraction field; Adopting glass fibre to twine epoxy resin is the column jecket matrix; Add that column jecket is the bunchy structure of two ends wall thickness intermediate thin, and process locked groove technology, optimized design greatly at two ends; The kayser fitted position is little, and sealing and pressure-bearing are reliable; Column jecket inside is the axial compression mode, and inner axial compression technology makes the preparative chromatography post can freely compress in use, and the number of plates of separation increases, and separating effect is better; And adopt the preparative chromatography post of column jecket parallel connection technology, can realize that the chromatographic column withstand voltage properties improves, can reach technical grade production demand, low cost of manufacture is easy to use simultaneously.

Description of drawings

Fig. 1 is the structural representation of the utility model chromatographic column one preferred embodiment;

Fig. 2 be C shown in Figure 1 to structural representation;

Fig. 3 is the embedded simple structural representation of column jecket in the utility model chromatographic column;

Fig. 4 is the internal structure sketch map of upper flange device in the utility model chromatographic column;

Fig. 5 is the internal structure sketch map of lower flange device in the utility model chromatographic column;

Fig. 6 is the internal structure sketch map of piston apparatus in the utility model chromatographic column;

Fig. 7 is the structural representation of another preferred embodiment of the utility model chromatographic column.

Among the figure: 1, column jecket; 2, upper flange device; 3, lower flange device; 4, piston apparatus; 5, spiral woven hose; 20, go up locked groove; 21, go up kayser; 22, pressurization mouth; 23, inlet; 24, pressure release exhaust outlet; 25, first sealing ring; 26, upper flange; 30, following locked groove; 31, following kayser; 32, filler is imported and exported; 33, liquid outlet; 34, first sieve plate; 35, cylinder manifold; 36, second sealing ring; 37, lower flange; 38, two pass ball valves; 40, the 3rd sealing ring; 41, piston; 42, flow distribution plate; 43, second sieve plate.

The specific embodiment

Below in conjunction with accompanying drawing the preferred embodiment of the utility model is set forth in detail, thereby the protection domain of the utility model is made more explicit defining so that advantage of the utility model and characteristic can be easier to it will be appreciated by those skilled in the art that.

See also Fig. 1, Fig. 2 and Fig. 3; The utility model embodiment comprises: a kind of chromatographic column comprises column jecket 1, upper flange device 2, lower flange device 3 and piston apparatus 4; Piston apparatus 4 is installed on column jecket 1 inside and in column jecket 1, carries out axial compression; Be connected with a spiral woven hose 5 between upper flange device 2 and the piston apparatus 4, the two ends pipe thickness of column jecket 1 body offers locked groove 20 and following locked groove 30 greater than the thickness of middle part tube wall at the two ends of column jecket 1; Last locked groove 20 is built-in with kayser 21 and following kayser 31 respectively with following locked groove 30, and last kayser 21 and following kayser 31 are pressed in upper flange device 2 and lower flange device 3 at the two ends of column jecket 1.

Further; See also Fig. 4; Said upper flange device 2 comprises pressurization mouth 22, inlet 23, pressure release exhaust outlet 24, first sealing ring 25 and upper flange 26; Inlet 23 is arranged in upper flange 26 and is connected with spiral woven hose 5, and pressurization mouth 22 and pressure release exhaust outlet 24 are arranged in upper end and UNICOM's piston 41 upper areas of upper flange 26, and upper flange 26 seals and be pressed on the port of column jecket 1 through first sealing ring 25 and last kayser 21; First sealing ring 25 prevents that for the sealing of upper flange 26 provides assurance liquid from flowing out.

See also Fig. 5; Said lower flange device 3 comprises that filler imports and exports 32, liquid outlet 33, first sieve plate 34, cylinder manifold 35, second sealing ring 36 and lower flange 37; Filler import and export 32 and liquid outlet 33 are arranged in the lower surface of lower flange 37, and cylinder manifold 35 is arranged on the upper end of lower flange 37, and first sieve plate 34 is arranged on the upper end of cylinder manifold 35; Lower flange 37 seals and is pressed on the lower port of column jecket 1 through second sealing ring 36 and following kayser 31; Exit at filler import and export 32 and liquid outlet 33 is respectively arranged with two pass ball valves, 38, the second sealing rings 36 for the sealing of lower flange 37 provides assurance, and filler is imported and exported 32 tops, first sieve plate 34 has a breach that filler is flowed into; Cylinder manifold 35 evenly converges the solvent in the column jecket 1, and flows out from liquid outlet 33.

See also Fig. 6; Said piston apparatus 4 comprises that the 3rd sealing ring 40, piston 41, flow distribution plate 42 and second sieve plate, 43, the three sealing rings 40 fit tightly with piston 41 and tube wall, and flow distribution plate 42 is arranged on the lower end of piston 41; Second sieve plate 43 is arranged on the lower end of flow distribution plate 42; The 3rd sealing ring 40 is shunted through flow distribution plate 42 from the material that piston 41 top spiral woven hoses 5 flow into for the sealing of piston 41 provides assurance, is being filtered inflow column jecket lower end, back by second sieve plate 43.

See also Fig. 7; For more extensively can having high input, the application that makes chromatographic column produces in batches; Said column jecket 1 is that a column jecket independently uses or a plurality of parallelly connected column jecket uses simultaneously; Said column jecket comprises liner and surrounding layer, and said liner is PP, PVDF or 316L stainless-steel thin-wall pipe, and said surrounding layer is for being substrate glass fiber winding process layer with the epoxy resin material.The column jecket thickness of glass fibre winding epoxy resin technology manufacturing can reach 50 millimeters in addition; Withstand voltagely can reach 40MPa; The column jecket internal diameter is (200 millimeters of conventional internal diameters) from 20 millimeters to 1000 millimeters, and the length maximum can reach 6 meters, basic satisfied existing large-scale chromatographic separation and purification and extraction.

The method of operating of the utility model chromatographic column: at first piston apparatus 4 is installed in the middle part in the column jecket 1; An end of spiral woven hose 5 connects piston apparatus 4 then; The other end is arranged in the inlet 23 of upper flange device 2; Upper flange device 2 is fixed in the upper end of column jecket 1, and lower flange device 3 is fixed in the lower end of column jecket 1; Squeezing into compression liquid in the pressurization mouth 22, by 24 exhausts of pressure release exhaust outlet, is that the piston upper end retains certain liquid; Be convenient to protect piston, then import and export 32 from filler and squeeze into filler homogenate, then pressurization mouth 22 is beaten liquid; Compression piston 41 compresses filler, gets final product life's work.

The utility model is different from prior art: the mode that adopts locked groove and kayser to combine seals and pressure-bearing flange; Having cancelled flange heavy in original tradition connects; Column tube material can tolerate most organic solvents, bronsted lowry acids and bases bronsted lowry; Realize the working method of a plurality of chromatographic column parallel connections, improved production efficiency.

The above is merely the embodiment of the utility model; Be not thus the restriction the utility model claim; Every equivalent structure or equivalent flow process conversion that utilizes the utility model specification and accompanying drawing content to be done; Or directly or indirectly be used in other relevant technical fields, all in like manner be included in the scope of patent protection of the utility model.

Claims

1. chromatographic column; Comprise column jecket, upper flange device, lower flange device and piston apparatus is characterized in that; Said piston apparatus is installed in the column jecket middle part and in column jecket, carries out axial compression; Be connected with a spiral woven hose between said upper flange device and the piston apparatus, the two ends pipe thickness of said column jecket body offers locked groove and following locked groove greater than the thickness of middle part tube wall at the two ends of said column jecket; The said locked groove of going up is built-in with kayser and following kayser respectively with following locked groove, and the said kayser of going up is pressed in the column jecket two ends to upper flange device and lower flange device with following kayser.

2. chromatographic column according to claim 1; It is characterized in that; Said upper flange device comprises pressurization mouth, inlet, pressure release exhaust outlet, first sealing ring and upper flange; Said pressurization mouth, inlet and pressure release exhaust outlet are arranged in the upper surface of upper flange, and said upper flange seals and be pressed on the port of column jecket through first sealing ring and last kayser.

3. chromatographic column according to claim 1; It is characterized in that; Said lower flange device comprises filler import and export, liquid outlet, first sieve plate, cylinder manifold, second sealing ring and lower flange; Said filler import and export and liquid outlet are arranged in the lower surface of lower flange, and said cylinder manifold is installed in the upper end of lower flange, and said first sieve plate is installed in the upper end of cylinder manifold; Said lower flange is through second sealing ring and the sealing of play kayser and be pressed on the lower port of column jecket, and the exit of said filler import and export and liquid outlet is respectively arranged with two pass ball valves.

4. chromatographic column according to claim 1; It is characterized in that; Said piston apparatus comprises the 3rd sealing ring, piston, flow distribution plate and second sieve plate; Said the 3rd sealing ring packed-piston and vial wall, said flow distribution plate is installed in the lower end of piston, and said second sieve plate is installed in the lower end of flow distribution plate.

5. chromatographic column according to claim 1 is characterized in that said column jecket comprises liner and surrounding layer, and said liner is PP, PVDF or 316L stainless-steel thin-wall pipe, and said surrounding layer is for being substrate glass fiber winding process layer with the epoxy resin material.

6. chromatographic column according to claim 1 is characterized in that, said column jecket is that a column jecket independently uses or a plurality of parallelly connected column jecket uses simultaneously.