CN1484972A - Anti-diarrhea yelk antibody feed additive for swine and injection formulation, and preparation method - Google Patents
Anti-diarrhea yelk antibody feed additive for swine and injection formulation, and preparation method Download PDFInfo
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Abstract
A feed additive containing yolk antibodies against diarrhea in pigs. The antibodies are preparing from bacilluscoli, epidemic diarrhea or rotavirus, or immunizing non-immunitive hens laying eggs with combined vaccine of bacilluscoli and/or epidemic diarrhea and /or rotavirus, then collecting yolks of the hens. A yolk antibody injecting agent, which is prepared from bacilluscoli, epidemic diarrhea or rotavirus, or immunizing non-immunitive hens laying eggs with combined vaccine of bacilluscoli and/or epidemic diarrhea and /or rotavirus, then collecting yolks of the hens.
Description
Technical field
The present invention relates to a kind of anti-diarrhea of pigs Yolk antibody feed additive and a kind of anti-diarrhea of pigs yolk antibody injection and preparation method thereof.
Background technology
Pig is a kind of important livestock products, as the food production resource, in people's life, occupy very heavy status, yet the M ﹠ M of pig is very high in China, especially southern each province, the mortality rate of pig reaches 8～12%, wherein major part is by due to the diarrhoeal diseases, and diarrhoeal diseases mainly is by escherichia coli, transmissible gastroenteritis, epidemic diarrhea and rotavirus cause, Developing of Animal Industry in serious harm, but for the diarrhea of pigs disease, we still rest on so far on the level of the passive treatment of a large amount of medications of symptom that just shows after the pig morbidity, ignore and improve the anti-preventive and therapeutic effect of former ability that cause a disease of pig self body diarrhoeal diseases, result: not only take a lot of work, effort, expend height, effect is also undesirable, some medicine even remain in the pig body, have a strong impact on the quality of livestock products, and a large amount of medications also cause pathogenic bacteria to produce drug resistance, are unfavorable for the control of disease.
Summary of the invention
Technical problem to be solved by this invention provides a kind ofly prevents and treats the Yolk antibody feed additive of diarrhoeal diseases and a kind ofly the antibody horizontal that causes the corresponding cause of disease of diarrhoeal diseases is prevented and treated yolk antibody injection of diarrhoeal diseases and preparation method thereof by improving pig body the antibody horizontal that causes the corresponding cause of disease of diarrhoeal diseases by improving pig body.
The present invention solves the technical scheme that its technical problem takes: thisly the antibody horizontal that causes the corresponding cause of disease of diarrhoeal diseases is prevented and treated the Yolk antibody feed additive of diarrhoeal diseases by improving pig body, promptly anti-diarrhea of pigs Yolk antibody feed additive, it is with swine escherichia coli, single Seedling of epidemic diarrhea or rotavirus preparation, or the non-immune bird inlay of connection Seedling vaccine immunity of swine escherichia coli and/or transmissible gastroenteritis and/or epidemic diarrhea and/or rotavirus preparation, regather immune bird inlay yolk preparation, the preparation method of this anti-diarrhea of pigs Yolk antibody feed additive is:
1, separates swine escherichia coli, epidemic diarrhea or rotavirus and carry out enrichment culture, after having antigenicity, be prepared into swine escherichia coli, epidemic diarrhea or rotavirus list Seedling, or separate swine escherichia coli and/or transmissible gastroenteritis and/or epidemic diarrhea and/or rotavirus and carry out enrichment culture, after having antigenicity, be prepared into swine escherichia coli and/or transmissible gastroenteritis and/or epidemic diarrhea and/or rotavirus connection Seedling vaccine;
2, with the swine escherichia coli, epidemic diarrhea or the rotavirus list Seedling that prepare, or non-immune bird inlay is carried out inoculation with the swine escherichia coli of preparation and/or transmissible gastroenteritis and/or epidemic diarrhea and/or rotavirus connection Seedling vaccine, the induction of immunity hen produces anti-swine escherichia coli, epidemic diarrhea or rotavirus antibody, or produces anti-swine escherichia coli and/or transmissible gastroenteritis and/or epidemic diarrhea and/or rotavirus antibody;
3, behind the immune bird inlay yolk of collection, dry again, promptly make anti-diarrhea of pigs Yolk antibody feed additive of the present invention.
The present invention solves another technical scheme that its technical problem takes: thisly the antibody horizontal that causes the corresponding cause of disease of diarrhoeal diseases is prevented and treated the yolk antibody injection of diarrhoeal diseases by improving pig body, promptly anti-diarrhea of pigs yolk antibody injection, it is with swine escherichia coli, single Seedling of epidemic diarrhea or rotavirus preparation, or the non-immune bird inlay of connection Seedling vaccine immunity of swine escherichia coli and/or transmissible gastroenteritis and/or epidemic diarrhea and/or rotavirus preparation, extract immune bird inlay yolk antibody preparation again, the preparation method of this anti-diarrhea of pigs yolk antibody injection is:
1, separates swine escherichia coli, epidemic diarrhea or rotavirus and carry out enrichment culture, after having antigenicity, be prepared into swine escherichia coli, epidemic diarrhea or rotavirus list Seedling, or separate swine escherichia coli and/or transmissible gastroenteritis and/or epidemic diarrhea and/or rotavirus and carry out enrichment culture, after having antigenicity, be prepared into swine escherichia coli and/or transmissible gastroenteritis and/or epidemic diarrhea and/or rotavirus connection Seedling vaccine;
2, with the swine escherichia coli, epidemic diarrhea or the rotavirus list Seedling that prepare, or non-immune bird inlay is carried out inoculation with the swine escherichia coli of preparation and/or transmissible gastroenteritis and/or epidemic diarrhea and/or rotavirus connection Seedling vaccine, the induction of immunity bird inlay produces anti-swine escherichia coli, epidemic diarrhea or rotavirus antibody, or produces anti-swine escherichia coli and/or transmissible gastroenteritis and/or epidemic diarrhea and/or rotavirus antibody;
3, behind the immune bird inlay egg yolk of collection, extract yolk antibody again, promptly make anti-diarrhea of pigs yolk antibody injection of the present invention.
Anti-diarrhea of pigs Yolk antibody feed additive provided by the present invention and anti-diarrhea of pigs yolk antibody injection, preparation technology is simple, cost is low, nontoxic, harmless, noresidue, pollution-free and easy to use, all be the antibody horizontal that causes the corresponding cause of disease of diarrhoeal diseases to be prevented and treated diarrhoeal diseases by improving pig body, not only convenient prevention feeding of anti-diarrhea of pigs Yolk antibody feed additive but also special suitable piglet are used, anti-diarrhea of pigs yolk antibody injection uses in pig morbidity back, the efficacy of a drug is more direct, drug effect is faster, compared with prior art: the measure of control diarrhea of pigs is convenient, flexibly, effective, and saving of labor, laborsaving, save money, with strong points, can avoid pig morbidity back drug abuse and cause medicine residual in the pig body, help the raising of livestock products quality, also can avoid the pig morbidity a large amount of passive medications in back and cause pathogenic bacteria to produce drug resistance, help the control of diarrhea of pigs disease, the actual use shows: all have good result of use and without any side effects.
The specific embodiment
The embodiment of the invention 1 provides a kind of anti-diarrhea of pigs Yolk antibody feed additive, it is the non-immune bird inlay of single Seedling immunity with the swine escherichia coli preparation, regather immune bird inlay yolk preparation, the preparation method of this anti-diarrhea of pigs Yolk antibody feed additive is:
1, separates swine escherichia coli and carry out enrichment culture, after having antigenicity, be prepared into swine escherichia coli list Seedling;
2, the swine escherichia coli list Seedling with preparation carries out inoculation to non-immune bird inlay, the anti-swine escherichia coli list Seedling 1ml of first immunisation when injection every chicken muscle or subcutaneous injection preparation, two Zhou Houzai carry out the inoculation second time with the dosage of first immunisation twice, carry out inoculation for the third time after two Zhou Houzai with the dosage of four times of first immunisation, later on carry out an inoculation with the dosage of first immunisation twice more every other month, the induction of immunity bird inlay produces anti-swine escherichia coli antibody;
3, collect the immune bird inlay yolk in inoculation for the third time week back after, dry again, promptly make at colibacillary anti-diarrhea of pigs Yolk antibody feed additive.
With one group of 25 age in days of the forage feed that has added this anti-diarrhea of pigs Yolk antibody feed additive piglet of just having weaned, then be the piglet oral challenge escherichia coli that itself and other one group of 25 age in days are just weaned, normal diet is fed again, just wean with 25 ages in days, normal diet is fed and not oral challenge, the piglet contrast of not injecting any medicine, observed the result 15:
| Grouping | Piglet quantity (head) | Feed for nursing | Counteracting toxic substances | The result |
| Test group | ????20 | Added the feedstuff of 5% anti-diarrhea of pigs yolk antibody feed additives | Escherichia coli | Not morbidity is normal |
| Matched group 1 | ????20 | Normal diet | Escherichia coli | Draw yellow-white feces |
| Matched group 2 | ????20 | Normal diet | ———— | Normally |
Added the piglet of the forage feed of this anti-diarrhea of pigs Yolk antibody feed additive, not morbidity behind the oral challenge escherichia coli, growing way is suitable with the piglet of not counteracting toxic substances, normal diet nursing, indifference, this anti-diarrhea of pigs Yolk antibody feed additive provided by the present invention causes that to escherichia coli diarrhoea has prevention effect, without any side effects.
The embodiment of the invention 2 provides a kind of anti-diarrhea of pigs Yolk antibody feed additive, it is the non-immune bird inlay of quadruple vaccine immunity with swine escherichia coli, transmissible gastroenteritis, epidemic diarrhea and rotavirus preparation, regather immune bird inlay yolk preparation, the preparation method of this anti-diarrhea of pigs Yolk antibody feed additive is:
1, separates swine escherichia coli, transmissible gastroenteritis, epidemic diarrhea and rotavirus and carry out enrichment culture, after having antigenicity, be prepared into swine escherichia coli, transmissible gastroenteritis, epidemic diarrhea and rotavirus quadruple vaccine;
2, swine escherichia coli with preparation, transmissible gastroenteritis, epidemic diarrhea and rotavirus quadruple vaccine carry out inoculation to non-immune bird inlay, the vaccine 1ml of first immunisation when injection every chicken muscle or subcutaneous injection preparation, two Zhou Houzai carry out the inoculation second time with the dosage of first immunisation twice, carry out inoculation for the third time after two Zhou Houzai with the dosage of four times of first immunisation, later on carry out an inoculation with the dosage of first immunisation twice more every other month, the induction of immunity bird inlay produces anti-swine escherichia coli, transmissible gastroenteritis, epidemic diarrhea and rotavirus antibody;
3, collect the immune bird inlay yolk in inoculation for the third time week back after, dry again, promptly make anti-diarrhea of pigs Yolk antibody feed additive at escherichia coli, transmissible gastroenteritis, epidemic diarrhea and rotavirus.
Just wean and the piglet of oral challenge escherichia coli, transmissible gastroenteritis, epidemic diarrhea and rotavirus with the feedstuff that has added this anti-diarrhea of pigs Yolk antibody feed additive and one group of 25 age in days of normal diet difference feeding, just wean with 25 ages in days, normal diet is fed and not oral challenge, the piglet contrast of not injecting any medicine, observed the result 15:
| Grouping | Piglet quantity (head) | Counteracting toxic substances | Feed for nursing | The result |
| Test group | ????10 | Escherichia coli, transmissible gastroenteritis, epidemic diarrhea and rotavirus | Added the feedstuff of 5% anti-diarrhea of pigs Yolk antibody feed additive | 10 healings |
| Matched group 1 | ????10 | Escherichia coli, transmissible gastroenteritis, epidemic diarrhea and rotavirus | Normal diet | Draw yellow-white gruel sample feces, dead 3 |
| Matched group 2 | ????10 | ??????———— | Normal diet | Normally |
After oral challenge piglet feeding has added the feedstuff of this anti-diarrhea of pigs Yolk antibody feed additive, suffer from diarrhoea very fast promptly the recovery from illness, growing way is suitable with the piglet of not counteracting toxic substances, normal diet nursing, no big difference, the diarrhoea that this anti-diarrhea of pigs Yolk antibody feed additive provided by the present invention causes escherichia coli, transmissible gastroenteritis, epidemic diarrhea and rotavirus has therapeutic effect, without any side effects.
The embodiment of the invention 3 provides a kind of anti-diarrhea of pigs yolk antibody injection, it is the non-immune bird inlay of bigeminy vaccine immunity with swine escherichia coli and Transmissible gastroenteritis virus preparation, extract immune bird inlay yolk antibody preparation again, the preparation method of this anti-diarrhea of pigs yolk antibody injection is:
1, separates swine escherichia coli and Transmissible gastroenteritis virus and carry out enrichment culture, after having antigenicity, be prepared into swine escherichia coli and Transmissible gastroenteritis virus bigeminy vaccine;
2, swine escherichia coli and the Transmissible gastroenteritis virus bigeminy vaccine with preparation carries out inoculation to non-immune bird inlay, the vaccine 1ml of first immunisation when injection every chicken muscle or subcutaneous injection preparation, two Zhou Houzai carry out the inoculation second time with the dosage of first immunisation twice, carry out inoculation for the third time after two Zhou Houzai with the dosage of four times of first immunisation, later on carry out an inoculation with the dosage of first immunisation twice more every other month, the induction of immunity bird inlay produces anti-swine escherichia coli and Transmissible gastroenteritis virus antibody;
3, collect the immune bird inlay egg in the one week back of inoculation for the third time, after the sterilization, under aseptic condition, break eggshell, remove Ovum Gallus domesticus album, after washing egg yolk and removing vitellinae membrana, receive immune bird inlay yolk, to receive then immune bird inlay yolk pour in the sterilized container, add acetic acid-sodium acetate buffer, quietly under 0～4 ℃ condition behind the homogenate put 3～4 hours or spend the night, remove precipitation, collect supernatant, add an amount of ammonium sulfate in the supernatant, quietly again under 0～4 ℃ condition put 3～4 hours or spend the night high speed centrifugation, collecting precipitation, with the injection water dilution, promptly make anti-diarrhea of pigs yolk antibody injection at escherichia coli and Transmissible gastroenteritis virus.
This anti-diarrhea of pigs yolk antibody injection, be used for the ablactation just of 25 ages in days and the piglet of oral challenge escherichia coli and Transmissible gastroenteritis virus, with the ablactation just of one group of 25 age in days oral challenge escherichia coli and Transmissible gastroenteritis virus, do not inject this anti-diarrhea of pigs yolk antibody injection piglet, one group of 25 age in days just weaned, normal diet is fed and not oral challenge, the piglet contrast of not injecting any medicine, observed the result 15:
| Grouping | Piglet quantity (head) | Counteracting toxic substances | Injection | The result |
| Test group | ????10 | Escherichia coli and Transmissible gastroenteritis virus | Inject anti-diarrhea of pigs yolk antibody injection 5ml | 10 healings |
| Matched group 1 | ????10 | Escherichia coli and Transmissible gastroenteritis virus | ———— | Draw yellow-white gruel sample feces |
| Matched group 2 | ????10 | ———— | ———— | Normally |
The oral challenge piggy injection behind this anti-diarrhea of pigs yolk antibody injection, suffer from diarrhoea very fast promptly the recovery from illness, growing way is suitable with the piglet of not counteracting toxic substances, normal diet nursing, no big difference, this anti-diarrhea of pigs yolk antibody injection provided by the present invention has therapeutic effect, without any side effects to the diarrhoea that escherichia coli and Transmissible gastroenteritis virus cause.
The embodiment of the invention 4 provides a kind of anti-diarrhea of pigs yolk antibody injection, it is the non-immune bird inlay of quadruple vaccine immunity with swine escherichia coli, transmissible gastroenteritis, epidemic diarrhea and rotavirus preparation, extract immune bird inlay yolk antibody preparation again, the preparation method of this anti-diarrhea of pigs yolk antibody injection is:
1, separates swine escherichia coli, transmissible gastroenteritis, epidemic diarrhea and rotavirus and carry out enrichment culture, after having antigenicity, be prepared into swine escherichia coli, transmissible gastroenteritis, epidemic diarrhea and rotavirus quadruple vaccine;
2, swine escherichia coli with preparation, transmissible gastroenteritis, epidemic diarrhea and rotavirus quadruple vaccine carry out inoculation to non-immune bird inlay, the vaccine 1ml of first immunisation when injection every chicken muscle or subcutaneous injection preparation, two Zhou Houzai carry out the inoculation second time with the dosage of first immunisation twice, carry out inoculation for the third time after two Zhou Houzai with the dosage of four times of first immunisation, later on carry out an inoculation with the dosage of first immunisation twice more every other month, the induction of immunity bird inlay produces anti-swine escherichia coli, transmissible gastroenteritis, epidemic diarrhea and rotavirus antibody;
3, collect the immune bird inlay egg in the one week back of inoculation for the third time, after the sterilization, under aseptic condition, break eggshell, remove Ovum Gallus domesticus album, after washing egg yolk and removing vitellinae membrana, receive immune bird inlay yolk, to receive then immune bird inlay yolk pour in the sterilized container, add acetic acid-sodium acetate buffer, quietly under 0～4 ℃ condition behind the homogenate put 3～4 hours or spend the night, remove precipitation, collect supernatant, add an amount of ammonium sulfate in the supernatant, quietly again under 0～4 ℃ condition put 3～4 hours or spend the night high speed centrifugation, collecting precipitation, with the injection water dilution, promptly make at escherichia coli, transmissible gastroenteritis, the anti-diarrhea of pigs yolk antibody injection of epidemic diarrhea and rotavirus.
This anti-diarrhea of pigs yolk antibody injection, be used for the ablactation just of 25 ages in days and oral challenge escherichia coli, transmissible gastroenteritis, epidemic diarrhea and rotavirus piglet, with the ablactation just of one group of 25 age in days oral challenge escherichia coli, transmissible gastroenteritis, epidemic diarrhea and rotavirus, do not inject this anti-diarrhea of pigs yolk antibody injection piglet, one group of 25 age in days just weaned, normal diet is fed and not oral challenge, the piglet contrast of not injecting any medicine, observed the result 15:
| Grouping | Piglet quantity (head) | Counteracting toxic substances | Injection | The result |
| Test group | ????10 | Escherichia coli, transmissible gastroenteritis, epidemic diarrhea and rotavirus | Inject anti-diarrhea of pigs yolk antibody injection 5ml | 10 healings |
| Matched group 1 | ????10 | Escherichia coli, transmissible gastroenteritis, epidemic diarrhea and rotavirus | ———— | Draw yellow-white gruel sample feces, dead 2 |
| Matched group 2 | ????10 | ———— | ———— | Normally |
The oral challenge piggy injection behind this anti-diarrhea of pigs yolk antibody injection, suffer from diarrhoea very fast promptly the recovery from illness, growing way is suitable with the piglet of not counteracting toxic substances, normal diet nursing, no big difference, the diarrhoea that this anti-diarrhea of pigs yolk antibody injection provided by the present invention causes swine escherichia coli, transmissible gastroenteritis, epidemic diarrhea and rotavirus has therapeutic effect, without any side effects.
Claims (8)
1, a kind of anti-diarrhea of pigs Yolk antibody feed additive, it is characterized in that: it is the single Seedling with swine escherichia coli, epidemic diarrhea or rotavirus preparation, or the non-immune bird inlay of connection Seedling vaccine immunity of swine escherichia coli and/or transmissible gastroenteritis and/or epidemic diarrhea and/or rotavirus preparation, regather immune bird inlay yolk preparation.
2, a kind of method for preparing the described anti-diarrhea of pigs Yolk antibody feed additive of claim 1 is characterized in that:
(1), separates swine escherichia coli, epidemic diarrhea or rotavirus and carry out enrichment culture, after having antigenicity, be prepared into swine escherichia coli, epidemic diarrhea or rotavirus list Seedling, or separate swine escherichia coli and/or transmissible gastroenteritis and/or epidemic diarrhea and/or rotavirus and carry out enrichment culture, after having antigenicity, be prepared into swine escherichia coli and/or transmissible gastroenteritis and/or epidemic diarrhea and/or rotavirus connection Seedling vaccine;
(2), with the swine escherichia coli, epidemic diarrhea or the rotavirus list Seedling that prepare, or non-immune bird inlay is carried out inoculation with the swine escherichia coli of preparation and/or transmissible gastroenteritis and/or epidemic diarrhea and/or rotavirus connection Seedling vaccine, the induction of immunity bird inlay produces anti-swine escherichia coli, epidemic diarrhea or rotavirus antibody, or produces anti-swine escherichia coli and/or transmissible gastroenteritis and/or epidemic diarrhea and/or rotavirus antibody;
(3), collect immune bird inlay yolk after, dry again, promptly make anti-diarrhea of pigs Yolk antibody feed additive of the present invention.
3, the method of the anti-diarrhea of pigs Yolk antibody feed additive of preparation according to claim 2, it is characterized in that: with the swine escherichia coli of preparation, epidemic diarrhea or rotavirus list Seedling, or non-immune bird inlay is carried out inoculation with the swine escherichia coli of preparation and/or transmissible gastroenteritis and/or epidemic diarrhea and/or rotavirus connection Seedling vaccine, the vaccine 1ml of first immunisation when injection every chicken muscle or subcutaneous injection preparation, two Zhou Houzai carry out the inoculation second time with the dosage of first immunisation twice, carry out inoculation for the third time after two Zhou Houzai with the dosage of four times of first immunisation, later on carry out an inoculation with the dosage of first immunisation twice more every other month, the induction of immunity bird inlay produces anti-swine escherichia coli, epidemic diarrhea or rotavirus antibody, or produce anti-swine escherichia coli and/or transmissible gastroenteritis and/or epidemic diarrhea and/or rotavirus antibody.
4, the method for the anti-diarrhea of pigs Yolk antibody feed additive of preparation according to claim 3, it is characterized in that: be after collecting the immune bird inlay yolk in the one week back of inoculation for the third time, dry again, make anti-diarrhea of pigs Yolk antibody feed additive of the present invention.
5, a kind of anti-diarrhea of pigs yolk antibody injection, it is characterized in that: it is the single Seedling with swine escherichia coli, epidemic diarrhea or rotavirus preparation, or the non-immune bird inlay of connection Seedling vaccine immunity of swine escherichia coli and/or transmissible gastroenteritis and/or epidemic diarrhea and/or rotavirus preparation, extract immune bird inlay yolk antibody preparation again.
6, a kind of method for preparing the described anti-diarrhea of pigs yolk antibody injection of claim 5 is characterized in that:
(1), separates swine escherichia coli, epidemic diarrhea or rotavirus and carry out enrichment culture, after having antigenicity, be prepared into swine escherichia coli, epidemic diarrhea or rotavirus list Seedling, or separate swine escherichia coli and/or transmissible gastroenteritis and/or epidemic diarrhea and/or rotavirus and carry out enrichment culture, after having antigenicity, be prepared into swine escherichia coli and/or transmissible gastroenteritis and/or epidemic diarrhea and/or rotavirus connection Seedling vaccine;
(2), with the swine escherichia coli, epidemic diarrhea or the rotavirus list Seedling that prepare, or non-immune bird inlay is carried out inoculation with the swine escherichia coli of preparation and/or transmissible gastroenteritis and/or epidemic diarrhea and/or rotavirus connection Seedling vaccine, the induction of immunity bird inlay produces anti-swine escherichia coli, epidemic diarrhea or rotavirus antibody, or produces anti-swine escherichia coli and/or transmissible gastroenteritis and/or epidemic diarrhea and/or rotavirus antibody;
(3), collect immune bird inlay yolk after, extract yolk antibody again, promptly make anti-diarrhea of pigs yolk antibody injection of the present invention.
7, the method of the anti-diarrhea of pigs yolk antibody of preparation according to claim 6 injection, it is characterized in that: with the swine escherichia coli of preparation, epidemic diarrhea or rotavirus list Seedling, or non-immune bird inlay is carried out inoculation with the swine escherichia coli of preparation and/or transmissible gastroenteritis and/or epidemic diarrhea and/or rotavirus connection Seedling vaccine, the vaccine 1ml of first immunisation when injection every chicken muscle or subcutaneous injection preparation, two Zhou Houzai carry out the inoculation second time with the dosage of first immunisation twice, carry out inoculation for the third time after two Zhou Houzai with the dosage of four times of first immunisation, later on carry out an inoculation with the dosage of first immunisation twice more every other month, the induction of immunity bird inlay produces anti-swine escherichia coli, epidemic diarrhea or rotavirus antibody, or produce anti-swine escherichia coli and/or transmissible gastroenteritis and/or epidemic diarrhea and/or rotavirus antibody;
8, the method of the anti-diarrhea of pigs yolk antibody of preparation according to claim 7 injection, it is characterized in that: be to collect the immune bird inlay egg in the one week back of inoculation for the third time, after the sterilization, under aseptic condition, break eggshell, remove Ovum Gallus domesticus album, after washing egg yolk and removing vitellinae membrana, receive immune bird inlay yolk, to receive then immune bird inlay yolk pour in the sterilized container, add acetic acid-sodium acetate buffer, quietly under 0～4 ℃ condition behind the homogenate put 3～4 hours or spend the night, remove precipitation, collect supernatant, add an amount of ammonium sulfate in the supernatant, quietly again under 0～4 ℃ condition put 3～4 hours or spend the night, high speed centrifugation, collecting precipitation with injection water dilution, makes anti-diarrhea of pigs yolk antibody injection of the present invention.
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| CNB031432468A CN1245888C (en) | 2003-08-12 | 2003-08-12 | Anti-diarrhea yelk antibody feed additive for swine and injection formulation, and preparation method |
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| CNB031432468A CN1245888C (en) | 2003-08-12 | 2003-08-12 | Anti-diarrhea yelk antibody feed additive for swine and injection formulation, and preparation method |
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| CN101948536A (en) * | 2010-05-10 | 2011-01-19 | 洛阳普莱柯生物工程有限公司 | Porcine transmissible gastroenteritis virus (PTGEV) and porcine epidemic diarrhea virus (PEDV) dual yolk antibody and preparation method thereof |
| CN102188449A (en) * | 2011-04-15 | 2011-09-21 | 广西壮族自治区兽医研究所 | Preparation method of high-immune whole-egg powder for resisting piglet diarrhea |
| CN102205122A (en) * | 2011-05-05 | 2011-10-05 | 广西壮族自治区兽医研究所 | Method for preparing piglet diarrhea-resisting egg yolk antibody microcapsules by spray drying method |
| CN102584990A (en) * | 2011-12-19 | 2012-07-18 | 李昌仁 | Method for extracting binary high-immunity yolk antibodies against newcastle disease and avian influenza |
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| CN102908620A (en) * | 2011-08-04 | 2013-02-06 | 广州格拉姆生物科技有限公司 | Preparation method of egg yolk antibody injection for treating piglet diarrhea |
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| CN104970224A (en) * | 2015-06-12 | 2015-10-14 | 青岛大信饲料有限公司 | Composition and mixed feed for preventing and treating porcine epizootic diarrhea |
| CN105494987A (en) * | 2015-12-30 | 2016-04-20 | 北京资源亚太饲料科技有限公司 | Feed composition capable of preventing and controlling piglet virus diarrhea and preparation method of feed |
| CN106822890A (en) * | 2017-01-19 | 2017-06-13 | 中国农业科学院兰州畜牧与兽药研究所 | A kind of pharmaceutical composition for preventing and treating diarrhea of weaned piglets and its preparation method and application |
| CN108850620A (en) * | 2018-08-27 | 2018-11-23 | 广州汇高生物科技有限公司 | A kind of special yolk immunoglobulin feed addictive and feed |
| CN111217906A (en) * | 2018-11-27 | 2020-06-02 | 郑洪杰 | Method for producing yolk antibody for preventing or treating swine digestive system disease, yolk antibody produced thereby, and use thereof |
| CN113845588A (en) * | 2021-05-26 | 2021-12-28 | 西南民族大学 | Preparation method and application of yolk antibody for resisting porcine rotavirus |
| CN114031684A (en) * | 2021-11-25 | 2022-02-11 | 重庆申金生物工程研究院有限公司 | Egg yolk antibody induced by porcine diarrhea-associated virus tandem epitope protein and application thereof |
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2003
- 2003-08-12 CN CNB031432468A patent/CN1245888C/en not_active Expired - Fee Related
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| CN101948536B (en) * | 2010-05-10 | 2013-07-03 | 普莱柯生物工程股份有限公司 | Porcine transmissible gastroenteritis virus (PTGEV) and porcine epidemic diarrhea virus (PEDV) dual yolk antibody and preparation method thereof |
| CN101948536A (en) * | 2010-05-10 | 2011-01-19 | 洛阳普莱柯生物工程有限公司 | Porcine transmissible gastroenteritis virus (PTGEV) and porcine epidemic diarrhea virus (PEDV) dual yolk antibody and preparation method thereof |
| CN102188449A (en) * | 2011-04-15 | 2011-09-21 | 广西壮族自治区兽医研究所 | Preparation method of high-immune whole-egg powder for resisting piglet diarrhea |
| CN102188449B (en) * | 2011-04-15 | 2012-12-19 | 广西壮族自治区兽医研究所 | Preparation method of high-immune whole-egg powder for resisting piglet diarrhea |
| CN102205122A (en) * | 2011-05-05 | 2011-10-05 | 广西壮族自治区兽医研究所 | Method for preparing piglet diarrhea-resisting egg yolk antibody microcapsules by spray drying method |
| CN102908620A (en) * | 2011-08-04 | 2013-02-06 | 广州格拉姆生物科技有限公司 | Preparation method of egg yolk antibody injection for treating piglet diarrhea |
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| CN111217906A (en) * | 2018-11-27 | 2020-06-02 | 郑洪杰 | Method for producing yolk antibody for preventing or treating swine digestive system disease, yolk antibody produced thereby, and use thereof |
| CN113845588A (en) * | 2021-05-26 | 2021-12-28 | 西南民族大学 | Preparation method and application of yolk antibody for resisting porcine rotavirus |
| CN113845588B (en) * | 2021-05-26 | 2023-06-09 | 西南民族大学 | Preparation method and application of yolk antibody for resisting porcine rotavirus |
| CN114031684A (en) * | 2021-11-25 | 2022-02-11 | 重庆申金生物工程研究院有限公司 | Egg yolk antibody induced by porcine diarrhea-associated virus tandem epitope protein and application thereof |
| CN114031684B (en) * | 2021-11-25 | 2024-06-04 | 重庆申金生物工程研究院有限公司 | Yolk antibody induced by tandem epitope protein of porcine diarrhea-associated virus and application thereof |
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