CN1472317A - Fermenting liquid for preparing clostridiopeptidase A - Google Patents
Fermenting liquid for preparing clostridiopeptidase A Download PDFInfo
- Publication number
- CN1472317A CN1472317A CNA021258783A CN02125878A CN1472317A CN 1472317 A CN1472317 A CN 1472317A CN A021258783 A CNA021258783 A CN A021258783A CN 02125878 A CN02125878 A CN 02125878A CN 1472317 A CN1472317 A CN 1472317A
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- CN
- China
- Prior art keywords
- fermented liquid
- preparing
- clostridiopeptidase
- gelatin
- collagenase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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Abstract
A fermenting liquid for preparing collagenase contains peptone (3-7 wt%), yeast cream (0.5-1.2), gelatin (1-3) and water (rest). Its advantages are no flating substance, easy dissolving, high fermenting effect and high output rate increased by 3-5%.
Description
Technical field
The invention belongs to field of biological pharmacy, relate in particular to a kind of fermented liquid that is used to prepare collagenase.
Background technology
The preparation method who from clostridium histolyticum's fermented liquid, extracts collagenase commonly used at present, as number of patent application is that the fermentation liquor formulation of introducing in the disclosed specification sheets of 85103913 " technology for making collagenase " is: yeast extract paste 0.2%-1%, yeast powder 0.2%-1%, gelatin 1%-3%, the aqueous solution of peptone 2%-6%.
This fermented liquid swims in the surface after adding yeast powder, is difficult for dissolving, influences ferment effect.
Summary of the invention
The purpose of this invention is to provide a kind of easy dissolving, ferment effect is good, high being used to of yield prepares collagenase fermented liquid.
The present invention is achieved in that the fermented liquid component (% by weight) of its preparation collagenase is peptone 3-7%, yeast extract paste 0.5-1.2%, and gelatin 1-3%, surplus is a water.The invention effect
Adopt the fermented liquid of above-mentioned component,, can not produce floating matter on the fermented liquid owing to do not add yeast powder, and easily dissolving, ferment effect is good, and its yield can improve 3-5%.
Embodiment
According to above-mentioned prescription, accurately take by weighing peptone 5%, yeast extract paste 0.8%, gelatin 2%, surplus are water, heating for dissolving, after treating all dissolvings, pour in the fermentor tank, add water then, making cumulative volume is 94% of requirement, and transferring pH with sodium hydroxide solution is 7.4, is heated to 121 ℃, sterilized 20 minutes, and be cooled to 37 ℃-41 ℃, just make fermented liquid, after stirring, stop to stir, be used to prepare collagenase.
Claims (2)
1. fermented liquid that is used to prepare collagenase, it is characterized in that: its component (% by weight) is: peptone 3-7%, yeast extract paste 0.5-1.2%, gelatin 1-3%, surplus is a water.
2. the fermented liquid that is used to prepare collagenase according to claim 1 is characterized in that: its component (% by weight) is: peptone 5%, and yeast extract paste 0.8%, gelatin 2%, surplus is a water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA021258783A CN1472317A (en) | 2002-08-01 | 2002-08-01 | Fermenting liquid for preparing clostridiopeptidase A |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA021258783A CN1472317A (en) | 2002-08-01 | 2002-08-01 | Fermenting liquid for preparing clostridiopeptidase A |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1472317A true CN1472317A (en) | 2004-02-04 |
Family
ID=34143120
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA021258783A Pending CN1472317A (en) | 2002-08-01 | 2002-08-01 | Fermenting liquid for preparing clostridiopeptidase A |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1472317A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101684461B (en) * | 2008-06-02 | 2014-08-20 | 霍夫曼-拉罗奇有限公司 | Improved purification of collagenases from clostridium histolyticum liquid culture |
-
2002
- 2002-08-01 CN CNA021258783A patent/CN1472317A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101684461B (en) * | 2008-06-02 | 2014-08-20 | 霍夫曼-拉罗奇有限公司 | Improved purification of collagenases from clostridium histolyticum liquid culture |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |