CN104357277B - A kind of preparation method of storing soft ecology pit mud Tiny ecosystem forced fluid - Google Patents
A kind of preparation method of storing soft ecology pit mud Tiny ecosystem forced fluid Download PDFInfo
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- CN104357277B CN104357277B CN201410629906.XA CN201410629906A CN104357277B CN 104357277 B CN104357277 B CN 104357277B CN 201410629906 A CN201410629906 A CN 201410629906A CN 104357277 B CN104357277 B CN 104357277B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
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- C12G3/02—Preparation of other alcoholic beverages by fermentation
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Abstract
The invention discloses the preparation method of a kind of storing soft ecology pit mud Tiny ecosystem forced fluid, belong to brewing technical field.The present invention is by extra inoculation ester-producing yeast and pit functional microorganism, guide the aging direction of storing soft ecology pit mud in sweat, improve microecosystem stability and the shortening aging required time of artificial distiller's yeast in brand-new cellar for storing things, promote the vinosity after going into operation in new cellar for storing things, excellent wine rate, it is achieved the new cellar for storing things excellent product of stable yields.Can apply to transform aging pit simultaneously.
Description
Technical field
The present invention relates to the preparation method of a kind of storing soft ecology pit mud Tiny ecosystem forced fluid, belong to brewing technical field.
Background technology
Mud cellar for storing things fermentation is aromatic Chinese spirit typical feature, and production has the saying of " wine has been produced at old cellar for storing things ", but needed for pit mud natural aging
Time is longer, simulation aged pit mud Tiny ecosystem and nutritional labeling manufacturing artificial pit mud so that the aging speed in new cellar for storing things is greatly improved.
At present, to adding ecological pit mud Tiny ecosystem forced fluid, brand-new pit mud micro-ecological environment is built, to improve the product of brand-new pit mud
The correlative study of matter is less, is a big technical barrier.
Summary of the invention
It is an object of the invention to strengthen storing soft ecology pit mud and make initial stage pit mud microecosystem, guide in sweat continuous soft
The aging direction of type ecology pit mud, improves microecosystem stability and the shortening aging required time of artificial distiller's yeast in brand-new cellar for storing things, carries
Rise the vinosity after going into operation in new cellar for storing things, excellent wine rate, it is achieved the new cellar for storing things excellent product of stable yields.Can apply to transform aging pit simultaneously.
In order to reach foregoing invention purpose, the invention provides the preparation method of a kind of storing soft ecology pit mud Tiny ecosystem forced fluid,
Mainly include some contents:
(1) described forced fluid matrix is counted in mass ratio and is contained: sodium acetate 0.2～0.5 part, K2HPO40.05～0.15 part, raw song
Face 2～4 parts, vinasse 1.5～3.5 parts, 95% alcohol 2～4 parts, peptone 0.5～1.5 parts, gold zone song powder 0.55～0.85
Part, little bent powder 0.5～1 part；Yellow water 6～8 parts；PH value 6.0～7.0.Wherein, gold zone song powder and the little Qu Fenwei saccharification stage
Adding, alcohol adds before inoculation.
Described raw curved surface is good quality wheat and barley in mass ratio 5～9:1 co-grinding is made.
Described vinasse are the fresh fragrant grain of storing soft, and color is pork liver redness or bronzing, and just, acidity is normal for fragrance alcohol, and the strength of calligraphic strokes is strong,
The growth of microorganism factor and regulation medium pH can be brought into.
Described gold zone song is made by high temperature Daqu is size-reduced, and wherein high temperature Daqu is at least stored 3 months after warehouse-in.
(2) by proportion ingredient in (1) (except gold zone song powder, little bent powder and alcohol), it is sequentially added in fermentation tank, limit edged
Stirring, adds water by mass volume ratio 1:10 (g/L)；It is heated to boiling, is cooled to 50～70 DEG C, add gold zone song powder and little
Qu Fen, stirs, saccharification while stirring 5～7h.
(3) in step (2), culture medium is cooled to 25～30 DEG C, inoculates ester-producing yeast bacterial classification, first aerobic fermentation by 5～10%
12～24h, make yeast amount reproduction, then anaerobic fermentation 36～48h, promote ester-producing yeast metabolism.Yeast is shape in metabolic process
Becoming metabolite precursor and growth factor, sterilized rear use, nutritional labeling is improved.
(4) zymotic fluid in step (3) is heated to boiling, after being cooled to 30～40 DEG C, adds alcohol, connect by 5～15%
The mixed bacteria liquid of kind of clostridium, bacillus licheniformis and ring-type gemma in culture medium, 30～37 DEG C of Anaerobic culturel 5～
8 days.
Described clostridium, bacillus licheniformis and the mixed bacteria liquid of ring-type gemma, be that three bacterial classifications are respectively taken a ring in bacterium
Culture medium is cultivated to logarithmic phase and obtain.
(5) zymocyte liquid 1:1 by volume～3 ratios step (4) obtained mix with pit mud leaching liquor, make ecology
Pit mud Tiny ecosystem forced fluid.
Described pit mud leaching liquor is that aged pit mud 1:10 in mass ratio adds residual water, and stirring stands, takes supernatant and get final product.
Gained storing soft ecology pit mud Tiny ecosystem forced fluid is yellow, smell be caproic acid be main compound fragrant, wherein total bacteria count is big
In 3 × 109Individual/mL, wherein bacillus is more than 3 × 108Individual/mL.In mass ratio 6～10% add in pit mud raw material and are given birth to
State pit mud, color is deeper, and shale is finer and smoother to be rubbed ripe, has certain cellar for storing things fragrance, more meets soft continuous white spirit and brewage requirement.Will
Brand-new pit mud is used for production of making wine, and produced base liquor top grade rate improves 5～10%.
When gained ecology pit mud Tiny ecosystem forced fluid is applied to fermentation, ecological pit mud Tiny ecosystem forced fluid can be added and become reconciled
Pit mud raw material in, carry out secondary and mud, proceed in fermentation vat, then surface add 10cm improve people's living condition state pit mud Tiny ecosystem strengthening
Liquid bacterium solution, fluid-tight is fermented.
In sum, pit mud Tiny ecosystem forced fluid provided by the present invention and culture process can solve problems with: (1) solves
New and pit mud microecosystem is unstable, the uncertain problem in direction of fermenting；(2) the most aging the asking of artificial pit is solved
Topic；(3) problem that pit is the most aging is solved；(4) problem that top grade wine can not be produced in new liter cylinder workshop is solved.
The present invention, by increasing pit mud Tiny ecosystem forced fluid in brand-new pit mud, improves functional microorganism advantage structure of community and physics and chemistry ring
Border, can provide good for later stage pit entry fermentation and ensure so that base liquor main body flavor substance dramatically increases, and mellow sense highlights, compound
Sense is strong, and style more becomes soft continuous white spirit standard.
Detailed description of the invention
Embodiment 1
A) Medium Proportion of storing soft ecology pit mud Tiny ecosystem forced fluid: sodium acetate 0.5 part；K2HPO40.2 part；Raw curved surface 4
Part；4 parts of vinasse；95% alcohol 3 parts；Peptone 1 part；Bent 0.6 part of gold zone；Little bent 0.9 part of powder；Yellow water 8 parts；PH value 7.0.
Wherein, the Qu Fenwei saccharification stage adds, and alcohol adds before inoculation.Raw curved surface is good quality wheat and barley in mass ratio 5～9:
1 co-grinding is made.Gold zone song preparation method is: life curved surface is first pressed into knee-piece, then accesses kind of a song, through about 3 months certainly
So gold zone song is made in fermentation, then stores through at least three moon, pulverizes and uses.
B) by proportion ingredient in step a) (except bent powder and alcohol), it is sequentially added in fermentation tank, stirring while adding, by quality
Volume ratio 1:10 is supplemented warm water or purifies water.It is heated to boiling, is cooled to 50 DEG C, add bent powder, stir, while stir
Mix limit saccharification 4h.
C) in step b), culture medium is cooled to 28 DEG C, produces Hansenula anomala, first aerobic fermentation 36h by 6% inoculation, makes yeast big
Amount breeding, then anaerobic fermentation 24h, promote ester-producing yeast metabolism.
D) zymotic fluid in step c) is heated to boiling, after being cooled to 35 DEG C, adds alcohol, by 6% inoculation shuttle shape gemma bar
The mixed bacteria liquid of bacterium, bacillus licheniformis and ring-type gemma in culture medium, 32 DEG C of Anaerobic culturel 5 days.
E) bacterium solution fermented in step d) 1:2 ratio by volume is mixed with pit mud leaching liquor, make the ecological micro-life of pit mud
State forced fluid.
F) the ecological pit mud Tiny ecosystem forced fluid in mass ratio 7% cultivated in step e) is added in the pit mud raw material become reconciled,
Carry out secondary and mud.Proceeding in fermentation vat, then add 10cm on surface and improve people's living condition state pit mud Tiny ecosystem forced fluid bacterium solution, fluid-tight is sent out
Ferment.
Gained storing soft ecology pit mud forced fluid is yellow, smell be caproic acid be main compound fragrant, wherein total bacteria count 4 × 109Individual
/ mL, wherein bacillus 5 × 108Individual/mL.The ecological pit mud color prepared is dark-grey, and shale is finer and smoother to be rubbed ripe, has certain
Cellar for storing things fragrance, more meets soft continuous white spirit and brewages requirement.Brand-new pit mud is used for production of making wine, and produced base liquor top grade rate improves 5%.
Embodiment 2
A) Medium Proportion of ecological pit mud Tiny ecosystem forced fluid: the Medium Proportion of ecological pit mud Tiny ecosystem forced fluid: sodium acetate
1 part；K2HPO40.4 part；Raw 8 parts of curved surface；4 parts of vinasse；95% alcohol 6 parts；Peptone 2 parts；1.2 parts of gold zone song powder；
Little bent 0.6 part of powder；Yellow water 6 parts；PH value 7.0.Wherein, the Qu Fenwei saccharification stage adds, and alcohol adds before inoculation.
B) by proportion ingredient in step a) (except bent powder and alcohol), it is sequentially added in fermentation tank, stirring while adding, by quality
Volume ratio 1:10 is supplemented warm water or purifies water.It is heated to boiling, is cooled to 50 DEG C, add bent powder, stir, while stir
Mix limit saccharification 4h.
C) in step b), culture medium is cooled to 28 DEG C, by 8% inoculation torulopsis, first aerobic fermentation 12h, makes yeast amount reproduction,
Anaerobic fermentation 48h again, promotes ester-producing yeast metabolism.
D) zymotic fluid in step c) is heated to boiling, after being cooled to 35 DEG C, adds alcohol, by 10% inoculation shuttle shape gemma bar
The mixed bacteria liquid of bacterium, bacillus licheniformis and ring-type gemma in culture medium, 32 DEG C of Anaerobic culturel 7 days.
E) the bacterium solution 1:1 ratio by volume fermented in just step d) mixes with pit mud leaching liquor, makes ecological pit mud micro-
Intensified ecological liquid.
F) the ecological pit mud Tiny ecosystem forced fluid in mass ratio 8% cultivated in step e) is added in the pit mud raw material become reconciled,
Carry out secondary and mud.Proceeding in fermentation vat, then add 10cm on surface and improve people's living condition state pit mud Tiny ecosystem forced fluid bacterium solution, fluid-tight is sent out
Ferment.
The storing soft ecology pit mud forced fluid prepared is yellow, smell be caproic acid be main compound fragrant, wherein total bacteria count 6 × 109
Individual/mL, wherein bacillus 6 × 108Individual/mL.Brand-new ecology pit mud color grey, shale is finer and smoother to be rubbed ripe, has certain
Cellar for storing things fragrance, more meets soft continuous white spirit and brewages requirement.Brand-new pit mud is used for production of making wine, and produced base liquor top grade rate improves 8%.
Embodiment 3
A) Medium Proportion of ecological pit mud Tiny ecosystem forced fluid: sodium acetate 1.5 parts；K2HPO40.6 part；Raw 12 parts of curved surface；
4 parts of vinasse；95% alcohol 9 parts；Peptone 3 parts；1.8 parts of gold zone song powder；Little bent 0.8 part of powder；Yellow water 7 parts；PH value 7.0.
Wherein, the Qu Fenwei saccharification stage adds, and alcohol adds before inoculation.
B) by proportion ingredient in step a) (except bent powder and alcohol), it is sequentially added in fermentation tank, stirring while adding, by quality
Volume ratio 1:10 is supplemented warm water or purifies water.It is heated to boiling, is cooled to 50 DEG C, add bent powder, stir, while stir
Mix limit saccharification 4h.
C) in step b), culture medium is cooled to 28 DEG C, by 10% inoculation ester-producing yeast bacterial classification, first aerobic fermentation 24h, makes yeast big
Amount breeding, then anaerobic fermentation 48h, promote ester-producing yeast metabolism.
D) zymotic fluid in step c) is heated to boiling, after being cooled to 35 DEG C, adds alcohol, by 10% inoculation shuttle shape gemma bar
The mixed bacteria liquid of bacterium, bacillus licheniformis and ring-type gemma in culture medium, 35 DEG C of Anaerobic culturel 6 days.
E) the bacterium solution 1:3 ratio by volume fermented in just step d) mixes with pit mud leaching liquor, makes ecological pit mud micro-
Intensified ecological liquid.
F) the ecological pit mud Tiny ecosystem forced fluid in mass ratio 9% cultivated in step e) is added in the pit mud raw material become reconciled,
Carry out secondary and mud.Proceeding in fermentation vat, then add 10cm on surface and improve people's living condition state pit mud Tiny ecosystem forced fluid bacterium solution, fluid-tight is sent out
Ferment.
The storing soft ecology pit mud forced fluid prepared is buff, smell be caproic acid be main compound fragrant, wherein total bacteria count 9 × 109
Individual/mL, wherein bacillus 8 × 108Individual/mL.Brand-new ecology pit mud color is taupe, and shale is finer and smoother to be rubbed ripe, has one
Fixed cellar for storing things fragrance, more meets soft continuous white spirit and brewages requirement.Brand-new pit mud is used for production of making wine, and produced base liquor top grade rate improves
10%.
Although the present invention is open the most as above with preferred embodiment, but it is not limited to the present invention, any person skilled in the art,
Without departing from the spirit and scope of the present invention, all can do various changes and modification, therefore protection scope of the present invention should be with
What claims were defined is as the criterion.
Claims (10)
1. a preparation method for storing soft ecology pit mud Tiny ecosystem forced fluid, mainly includes herein below:
(1) described forced fluid matrix is counted in mass ratio and is contained: sodium acetate 0.2～0.5 part, K2HPO40.05～0.15 part, raw song
Face 2～4 parts, vinasse 1.5～3.5 parts, 95% alcohol 2～4 parts, peptone 0.5～1.5 parts, gold zone song powder 0.55～0.85
Part, little bent powder 0.5～1 part；Yellow water 6～8 parts；PH value 6.0～7.0；
(2) by proportion ingredient in (1), except gold zone song powder, little bent powder and alcohol, other are sequentially added in fermentation tank, Bian Jia
Limit is stirred, and every g material adds 10L water；It is heated to boiling, is cooled to 50～70 DEG C, add gold zone song powder and little bent powder, stir
Mix uniformly, saccharification while stirring 5～7h；
(3) in step (2), culture medium is cooled to 25～30 DEG C, inoculates ester-producing yeast bacterial classification, first aerobic fermentation by 5～10%
12～24h, make yeast amount reproduction, then anaerobic fermentation 36～48h, promote ester-producing yeast metabolism；
(4) zymotic fluid in step (3) is heated to boiling, after being cooled to 30～40 DEG C, adds alcohol, connect by 5～15%
The mixed bacteria liquid of kind of clostridium, bacillus licheniformis and ring-type gemma in zymotic fluid, 30～37 DEG C of Anaerobic culturel 5～
8 days；
(5) the zymocyte liquid 1:1 by volume～3 that step (4) obtains is mixed with pit mud leaching liquor, make ecological pit mud
Tiny ecosystem forced fluid.
Method the most according to claim 1, it is characterised in that described raw curved surface be good quality wheat and barley in mass ratio 5～
9:1 co-grinding is made.
Method the most according to claim 1, it is characterised in that described vinasse are the fresh fragrant grain of storing soft, and vinasse color is
Pork liver redness or bronzing, just, acidity is normal for fragrance alcohol, and the strength of calligraphic strokes is strong, can bring the growth of microorganism factor and regulation medium pH into.
Method the most according to claim 1, it is characterised in that described gold zone song is made by high temperature Daqu is size-reduced,
Described high temperature Daqu is at least through warehouse-in storage 3 months.
Method the most according to claim 1, it is characterised in that described clostridium, bacillus licheniformis and ring-type
The mixed bacteria liquid of gemma, is three bacterial classifications respectively to take a ring cultivate in bacteria culture media to logarithmic phase and obtain.
Method the most according to claim 1, it is characterised in that described pit mud leaching liquor is by aged pit mud 1:10 in mass ratio
Adding residual water, stirring stands, takes supernatant and get final product.
Method the most according to claim 1, it is characterised in that preparation process belongs to semi open model, can inoculate brewery naturally
Microorganism in air.
The storing soft ecology pit mud Tiny ecosystem forced fluid that the most according to claim 1, method prepares.
Storing soft ecology pit mud Tiny ecosystem forced fluid the most according to claim 8, it is characterised in that for yellow, smell is
Caproic acid is main compound fragrant, and wherein total bacteria count is more than 3 × 109Individual/mL, wherein bacillus is more than 3 × 108Individual/mL.
10. the method that storing soft ecology pit mud Tiny ecosystem forced fluid described in application claim 8 prepares pit mud, it is characterised in that
Forced fluid in mass ratio 6～10% are added to pit mud raw material obtains ecological pit mud.
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| CN105623964B (en) * | 2016-03-13 | 2019-12-13 | 中国食品发酵工业研究院有限公司 | Technological measure for relieving pit mud calcification problem |
| CN106906099B (en) * | 2017-04-13 | 2020-05-08 | 山东梁山徐坊大曲有限公司 | Preparation method of pit mud fermentation accelerant and method for producing pit mud by using pit mud fermentation accelerant |
| CN107502510B (en) * | 2017-09-27 | 2020-10-23 | 重庆市重粮酒业有限公司 | Pit quality-improving and efficiency-increasing process |
| CN111961620B (en) * | 2020-08-19 | 2022-05-20 | 江苏洋河酒厂股份有限公司 | Compound microbial agent and application thereof in regulating pH of pit mud |
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| KR101146743B1 (en) * | 2009-11-25 | 2012-05-17 | 강병선 | Ice jujube wine and process for preparing the same |
| CN101914448A (en) * | 2010-08-10 | 2010-12-15 | 四川省绵阳市丰谷酒业有限责任公司 | Pit mud compound functional bacterial liquid and preparation method thereof |
| CN102839077B (en) * | 2012-09-12 | 2014-03-26 | 安徽省金裕皖生物科技开发有限公司 | Esterifying enzyme compound bacteria liquid preparation method |
| CN103173308A (en) * | 2013-03-07 | 2013-06-26 | 江苏洋河酒厂股份有限公司 | Cellar micro-ecology adjustment agent and adjustment method thereof |
| CN103160423A (en) * | 2013-03-07 | 2013-06-19 | 江苏洋河酒厂股份有限公司 | Composite function liquid and an application method |
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