CN1471828A - Selenium-riched leben and producing method thereof - Google Patents
Selenium-riched leben and producing method thereof Download PDFInfo
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Abstract
A selenium-enriched sour milk is prepared from the milk of cow or ewe through dissolving sodium selenite in sterilized distilled water, and traditional production process of sour milk, in whith the prepared Se solution is added. It can supplement Se to human body.
Description
Technical field
The invention belongs to dairy products and process technology thereof
Background technology
Yoghourt Production has history in thousand, and raw material can be bright ox, goat milk, and traditional Yoghourt Production technological process comprises batching, filtration, concentrates, operations such as preheating, homogeneous, sterilization, cooling, inoculation, fermented and cultured, product cooling.Sour milk nutrition is abundant, easily is absorbed by the body.Particularly contain lactic acid bacteria and lactase and make in the milk nutrient give digestion, make lactose be converted into lactic acid, make protein be able to hydrolysis, increase milk Ruzhong solubility calcium phosphorus and vitamin content, adapt to children and the elderly, have natural calcium supplementing effect the absorption of calcium phosphorus.
For a long time, people's imagination reinforced micro-element selenium from nutritious sour milk can be mended selenium in the hope of human body when absorbing the sour milk nutrient.Selenium generally enters human body by food chain, and selenium and local food book on Chinese herbal medicine are closely related.Research report " China's height selenium soil origin cause of formation an is analysed " literary composition according to Wang Meizhu, Zhang Mingkui points out that it is wide that China lacks the selenium area, and the main matrix that China lacks selenium soil is quaternary sediment, the formed soil of alluvial deposits.Serious selenium deficiency disease appears in some areas of China.Nasopharyngeal carcinoma district occurred frequently of the liver cancer district occurred frequently in the keshan disease districts in about more than 300 areas, the whole nation such as northeast Keshan, Zou County, Shandong, Qidong, Jiangsu, colorectal cancer district occurred frequently, Jiashan, Zhejiang, Lin County, Henan or the like for example, all relevant with parent rock, the scarce selenium of soil.In a word, populous at scarce selenium of China or the not enough area of selenium, mending selenium is the requisite measure of these regional population healths.So,, be the healthy Gospels of people really if in the sour milk that people extensively drink, realize mending selenium.But all do not obtain result so far with practical significance.For example invention disclosed title " a kind of fresh milk and goods thereof that are rich in organic selenium " number of patent application is 971061998 technology.It proposes, and selenium content is the 3-250 micrograms per litre in the sour milk, and this contains the selenium scope, and the boundary gap is too big up and down, differs 85 times, and in so wide scope, no implementation column is enumerated the technical characterictic value.The selenium content of lower limit 3 micrograms per litre is too low, does not reach to mend the selenium standard mesh.And the selenium content of higher limit is too high, is unsuitable for children and drinks, if children drink 100 milliliters of sour milks every day, 25 microgram selenium are just arranged in body, moreover the normal children drinks one bottle in one day incessantly.If drink the 2-3 bottle, the selenium of 75 micrograms of then having entered shows too high again.The production method of rich selenic acid milk in this documents, selenium content can not be fixed in each batch fresh milk (sour milk) that is provided.Because it is by the ox of breast-feeding with vinasse, the selenium in the vinasse is converted into organic selenium in the cow's milk, again by traditional production technology, produce rich selenic acid milk.And vinasse are byproducts of Beer Brewage, and selenium content depends on the barley of using when producing beer, the height that rice contains selenium, and its selenium content depends on the selenium content in the parent rock, soil, water source in the place of production.From different places, its selenium content difference is very big, can differ several times to hundred times, even thousands of times.So the selenium content of the rich selenic acid milk of so producing is difficult to obtain fixed index, and actual market value can not be arranged.And for example denomination of invention is " Bioconverted selenium-enriched milk and a production method thereof ", and number of patent application is that 00122955.9 the disclosed rich selenium fresh milk selenium content of documents is 0.020-0.030mg/kg (selenium content of corresponding its milk powder is 0.1-0.15mg/kg).Such selenium content scope makes that rich selenic acid milk is obviously on the low side, and people are few more than milk to the amount of drinking of sour milk, and that general is 100ml, 200-250ml etc.Can not satisfy the needs that people mend selenium so enter the selenium of human body.Its production method is selenium dioxide or sodium selenite or selenium yeast and amino acid one class material to be made into liquid mixture matter mutually make milk cow forage, by milk cow selenium in the feed is changed into selenium in the milk, like this conversion ratio of selenium and milk in selenium content really qualitative value must inquire into.For a long time with high selenium amount forage feed milk cow, milk cow easily suffers from selenosis disease, also is worthy of consideration even more noteworthy.And for example its selenium content that openly what is claimed is in the every 100ml milk of production of documents " selenium-rich milk product " (application number is 98120442.2) is the 5-100 microgram.The Se content of this milk is unreasonable, and its higher limit is that the danger of poisoning is arranged to being grown up, and its danger is bigger concerning children's milk drink.Though differ bigger between the bound, do not have to implement to enumerate the characteristic value of bright qualification.It is planted nutrient No. 8 (liquors) and produces selenium-rich milk with adding to contain in the selenium.
Summary of the invention:
Rich selenic acid milk of the present invention is the problem that exists at prior art, provides a kind of selenium content rational sour milk, can be applicable to that all ages and classes section, different health crowd keep healthy to mend the requirement of selenium; And invention creates the production method that the sour milk of organic selenium is stablized, is rich in to selenium content.Adopt technical scheme as follows for achieving the above object:
A kind of rich selenic acid dairy products, it is the sour milk that is rich in organic selenium that a kind of bio-transformation of passing through lactic acid bacteria of fermented type forms.With meet standardized ox, goat milk is raw material, the weight content of selenium is 0.060-0.130mg/kg in the sour milk.For satisfying the benefit selenium needs of different crowd, its selenium content also comprises centrifugal pump 0.160mg/kg, 0.190mg/kg, 0.220mg/kg outside above-mentioned scope.
Be exclusively used in the production method of this product, be in the traditional yogurt technological process of production: the standardization of milk raw material, batching, filtration, concentrate, on the basis of operations such as preheating, homogeneous, sterilization, cooling, inoculation, fermented and cultured, product cooling, increase following feature operation:
One, is producing of selenium solution
(1) selected selenium source is an inorganic selenium---sodium selenite (A, R or C, P level) chemical reagent;
(2) calculating of sodium selenite consumption is determined by following formula.
G＝R·N·M
The total consumption of required sodium selenite (mg) when G suckles for producing rich selenic acid in the formula
N contains selenium concentration (selenium is weight content mg/kg in sour milk) in the rich selenic acid milk
M is for producing sour milk gross weight (kg)
R is a transformation ratio 2.19;
(3) the preparation sodium selenite solution with the required sodium selenite that calculates, is mixed with the selenium solution that concentration is 0.2-0.4% with sterile purified water.Concentration is crossed to hang down and is easily made moisture content too much, and excessive concentration can cause defectives such as selenium maldistribution.
Two, in the Yoghourt Production technological process, above selenium solution is added in the raw milk, can select a place at following two places arbitrarily
(1) in burden process, adds selenium solution after the sugaring.This solution is added in the raw milk gradually, constantly stirs, treat that whole selenium solutions add after, continue to stir ten minutes, (purpose that requires to stir be for prevention in raw milk owing to the selenium maldistribution causes sour milk part " red selenium ", go out waste product and can see P6 for details.
(2) after sterilization cooling, add selenium solution before the inoculation operation, need after adding to continue to stir 15 minutes, selenium is evenly distributed in emulsion fully after, can inoculate (stirs 15 minutes reason (1)) together
Rich selenic acid of the present invention is suckled, and can satisfy the demand of different crowd, in technique scheme, gets selenium content at 0.060-0.130mg/kg, is the rich selenic acid milk of child form; If selenium content gets 0.160,0.190,0.220mg/kg, then be that the rich selenic acid of adult type is suckled.
The production method technology that adopts is simple, with low cost, and the selenium content of rich selenic acid milk is stable, easily control.
The sodium selenite that adds among the present invention forms the mechanism of organic selenium under the lactic acid bacteria effect:
1, solid-state---the formation of organic selenoprotein.
Selenoprotein mainly forms in lactic acid bacteria fermentation process in the rich selenic acid milk.The sweat of sour milk, the logarithmic growth of lactic acid bacteria the propagation phase is the breeding peak just.The sodium selenite that adds enters thalline by selenium concentration difference between raw milk and the lactic acid thalline, under the plurality of enzymes effect, forms organic selenium of amino attitude in vivo, further synthetic thereafter selenoprotein.This selenoprotein is the part of lactic acid thalline oneself protein matter, and it is utilized by growth, thalline division, the breeding of lactic acid bacteria, and this part selenium is to be fixed in the lactic acid thalline of new, old and dead (not being situated between by corruption).Be the component of organic selenium in the rich selenic acid milk, the breeding quantity of lactic acid bacteria is appreciable, and for example, when producing rich selenic acid milk, in each bottle (100 gram) raw milk, needing the inoculating lactic acid bacterium amount be 3 * 10
8Individual/ml, promptly 3,000 ten thousand.But through the sweat of sour milk, form rich selenic acid dairy products after, in its every bottle (100 gram) sour milk, lactic acid bacteria number can be bred to 100 * 10
8Individual/ml, promptly 1,000,000,000.(annotate: 10
8Individual/ml is that sour milk requires the microbiological indicator that reaches when dispatching from the factory).This shows, the number of lactic acid bacteria propagation and the notion of selenoprotein formation amount, they are organic selenium main bodys in the rich selenic acid milk.But lactic acid bacteria neither be endless to the demand of selenium, tests discovery according to the inventor, and when an amount of supply selenium, lactobacter growth is good, division is fast.The blush particle deposition occurs when concentration of sodium selenite in the emulsion is increased to more than the 4.2mg/kg in (comprise 4.2,5.5,6.6,8.8mg/kg) its sour milk, strengthen with concentration and increase, and it is red to have formed a slice.Reason be lactobacillus cell with the sodium selenite excessive, that toxicity is big, be reduced into the simple substance selenium of redness (fine granularity, nontoxic).Be deposited in the lactobacillus cell, this phenomenon can be understood as a kind of detoxifcation reaction of lactobacillus cell.When the sodium selenite amount is increased sharply when impotentia is detoxified lactic acid bacteria death.Form the sour milk of " red selenium ", can not eat, because simple substance selenium does not have nutritive value to human body.
This result of the test explanation (1) lactic acid bacteria needs selenium in the sodium selenite, but requirement is little, many can poisoning.(2) this patent is in adding the selenium technology contents, when lay special stress on adds selenium, must stir, add after the selenium liquid, also to append the requirement of stirring 10 minutes and 15 minutes, its objective is and in the not high raw milk of selenium content, stir unevenly, cause selenium local allocation inequality, cause " red selenium " and go out waste product by what for prevention.
The formation of 2, soluble state---organic selenium:
In rich selenic acid milk forming process, part sodium selenite is in the lactic acid thalline, under the effect of plurality of enzymes, formed the amino-acid state organic selenium compounds, wherein main component is a selenium cystine, and the liquid organic selenium compounds of selenomethionine class can be present in the sour milk by infiltration, also can further synthesize selenoprotein in the lactic acid thalline, this part selenium also is one of component of the organic selenium of sour milk.
3, inorganic selenium, in rich selenic acid milk production process, the sodium selenite that still has part not utilized by lactic acid bacteria, these selenium amounts belong to unemployed a spot of inorganic selenium source in the sour milk, and belong to one of component of total selenium amount.
Selenium is made up of above-mentioned three kinds of institutes in the rich selenic acid milk, these selenium enter the human intestines and stomach with sour milk, at the large intestine intracellular metabolite, lactic acid bacteria death in the human body large intestine of 2/3 is wherein arranged approximately, these death contain the selenium lactic acid bacteria, under the effect of protease, spoilage organisms, resolve into the organic selenium of soluble state and a spot of inorganic selenium, these selenium are absorbed in small intestine with original soluble state selenium is the same, enter the nutrition circulation of human body, said process is exactly formation, nutrition, the absorption process of rich selenic acid milk.Drink rich selenic acid milk of the present invention, can mend the selenium health care, very safe again.
Description of drawings
Accompanying drawing is the whole activity relation schematic diagrames of the technological process of production of the present invention.
The specific embodiment:
Feature process step of the present invention is to be that the selenium solution of 0.2-0.4% is evenly added in designating step with concentration.As shown in the figure, can add an optional place at two places.Wherein adding No. 1 position of selenium adds in the intact back of burden process sugaring; Adding No. 2 positions of selenium is after the sterilization refrigerating work procedure, adds selenium solution before the inoculation operation.The inventor thinks that it is good considering to select No. 1 position to add selenium from the selenium degree that is evenly distributed emulsion.Further specify the embodiment of rich selenic acid dairy products of the present invention and production method thereof with reference to the accompanying drawings.
Example 1:
Require to produce rich selenic acid milk 2000 grams, the weight content of its selenium is 0.060mg/kg, and its production craft step is as follows:
1, preparation selenium solution
(1) calculates the total consumption of sodium selenite by relational expression G=RNM
G＝2.19×0.060×2＝0.263mg
(2) sodium selenite of 0.263mg is mixed with the selenous acid solution of 0.2% concentration, takes by weighing chemical pure A, R level sodium selenite 0.263mg, place in the sterilized 500CC beaker, add sterile purified water 132ml, constantly stir, treat to dissolve fully, promptly get 0.2% sodium selenite solution.
2, produce the operation of rich selenic acid milk
(1) gets raw milk batching (sugaring, add selenium)
Get raw milk 2000 grams after the standardization, be heated to 55-65 ℃, equably 160 gram sugar (sucrose 140 grams mix with glucose 20 grams) are added in the raw milk and constantly stir, treat to dissolve fully, 0.2% sodium selenite solution 132ml is added gradually in the raw milk of sugaring and constantly and stir, after adding selenium solution, continue to stir ten minutes;
(2) with the raw milk after the above-mentioned batching through concentrating, after the operations such as filtration, sterilization, cooling, when being cooled to 42-43 ℃, enter the inoculation operation, with working stock culture (mixing at 1: 1 with the thermophilic lotus coccus) 60ml of mixed type, add constantly to stir after 10-15 minute in the raw milk and carry out packing (200 gram/bottle) with Bulgarian Lactobacillus.
(3) fermented and cultured, raw milk to be fermented after the packing is placed in 43 ℃ of biochemical incubators cultivated 4 hours, PH reduces to 4.3-4.4 when the fermentation emulsion, acidity is that 0.8T is when spending, put immediately to 15-20 degree environment and cool off, treat to change over to again when the acidified milk temperature is reduced to 15-20 ℃ 2-8 ℃ of refrigerator refrigeration, after-ripening can form the rich selenic acid milk finished product with excellent flavor in 24 hours, this product after measured its to contain selenium be to be 0.069mg/kg, with the deviation of 0.060mg/kg be by test error etc. produce.
Example 2:
Require to produce rich selenic acid milk 2000 grams that selenium content is 0.090mg/kg
1, preparation selenium solution
Calculating the sodium selenite consumption is 0.394mg, adds sterile purified water 197ml, is mixed with uniform 0.2% sodium selenite solution.
2, production process is with (1), (2), (3) in the 2nd of the example 1
Getting product selenium content measured value is 0.098mg/kg.
Example 3:
Require to produce rich selenic acid milk 2000 grams that selenium content is 0.12mg/kg
1, calculating the sodium selenite consumption is 0.526mg, is mixed with concentration and is 0.2% sodium selenite solution.
2, production process is with (1), (2), (3) in the 2nd of the example 1
Getting rich selenic acid dairy products selenium content measured value is 0.126mg/kg.
Example 4:
1, require to produce and to contain the rich selenic acid milk of 2000 grams that the selenium total amount is 0.160mg/kg, calculating the selenous acid consumption is 0.711mg, adds sterile purified water 237ml, fully after the dissolving concentration is 0.3% sodium selenite solution.
2, production process is with (1), (2), (3) in the 2nd of the example 1
Getting the product selenium content is 0.155mg/kg.
Example 5:
Require to produce rich selenic acid milk 2000 grams that selenium content is 0.190mg/kg
1, calculate to such an extent that the sodium selenite consumption is 0.832mg, get sterile purified water 274ml, concentration is 0.3% sodium selenite solution.
2, processing step is with (1), (2), (3) in the 2nd of the example 1
Getting selenium content is the rich selenic acid milk of 0.183mg/kg.
Example 6:
Require to produce rich selenic acid milk 2000 grams that selenium content is 0.22mg/kg
1, calculate to such an extent that the sodium selenite consumption is 0.964mg, get sterile purified water 322ml, concentration is 0.3% sodium selenite solution.
2, processing step is with (1), (2), (3) in the 2nd of the example 1
Getting selenium content is the rich selenic acid milk of 0.216mg/kg.
Claims (7)
1, a kind of rich selenic acid milk, raw material is got ox, goat milk, and the weight content scope that it is characterized in that selenium is 0.060-0.130mg/kg.
2, by the described rich selenic acid milk of claim 1, the content that it is characterized in that selenium is 0.090mg/kg.
3, by the described rich selenic acid milk of claim 1, the content that it is characterized in that selenium is 0.120mg/kg.
4, a kind of rich selenic acid milk is characterized in that selenium content is 0.160mg/kg.
5, a kind of rich selenic acid milk is characterized in that selenium content is 0.190mg/kg.
6, a kind of rich selenic acid milk is characterized in that selenium content is 0.220mg/kg.
7, the production method that is used for the rich selenic acid milk of claim 1 or 4 or 5 or 6, use the Yoghourt Production technological process: raw milk standardization, batching, filtration, concentrate, preheating, homogeneous, sterilization, cooling, inoculation, fermented and cultured, product cooling, after-ripening, the feature operation of its increase is by following operation:
One, selenium solution is produced
(1) selected selenium source is a sodium selenite
(2) calculating of sodium selenite consumption is determined by following formula
G＝R·N·M
The consumption (mg) of required sodium selenite when G suckles for producing rich selenic acid in the formula
N contains selenium concentration (mg./kg) in the rich selenic acid milk
M is for producing sour milk gross weight (kg)
R is a transformation ratio 2.19.
(3) the inferior selenium sodium solution of preparation is mixed with the selenium solution that concentration is 0.2-0.4% with required sodium selenite with sterile purified water.
Two, in the Yoghourt Production technological process, above selenium solution is added in the raw milk, can choose a place at two places wantonly.
(1) in burden process after the sugaring, add selenium solution; Add gradually in the raw milk, constantly stir, treat that whole selenium solutions add after, continue to stir ten minutes.
(2) behind the sterilization refrigerating work procedure, inoculation adds selenium solution before the operation, needs after adding to continue to stir 15 minutes, can inoculate after selenium is evenly distributed in emulsion fully.
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| CN1471828A true CN1471828A (en) | 2004-02-04 |
| CN1317964C CN1317964C (en) | 2007-05-30 |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100379346C (en) * | 2005-11-22 | 2008-04-09 | 余内逊 | Method for preparing sour milk contg. micron sugared ginseng, bee-glue etc. for tonifying-Yin and nourishing-stomach |
| CN102138622A (en) * | 2011-01-10 | 2011-08-03 | 苏州硒谷科技有限公司 | Cold drink food rich in selenium substituted amino acid and preparation method thereof |
| CN102613297A (en) * | 2012-04-17 | 2012-08-01 | 光明乳业股份有限公司 | Milk for enhancing organism immunity and preparation process of milk |
| CN102771563A (en) * | 2012-08-15 | 2012-11-14 | 苏州硒谷科技有限公司 | Selenium-enriched milk product and production method thereof |
| CN104255916A (en) * | 2014-09-18 | 2015-01-07 | 印天寿 | Selenium-enriched yogurt and family preparation method of selenium-enriched yogurt |
| CN105558011A (en) * | 2015-12-16 | 2016-05-11 | 新希望双喜乳业（苏州）有限公司 | Set-style yoghurt capable of enhancing organism immunity and preparation method thereof |
| CN107494731A (en) * | 2017-09-27 | 2017-12-22 | 光明乳业股份有限公司 | A kind of selenium-enriched probioticses acidified milk and preparation method thereof |
| CN107593912A (en) * | 2017-10-13 | 2018-01-19 | 东北农业大学 | A kind of high activity Selenium-enriched fermentation goat milk beverage and preparation method thereof |
| CN112674167A (en) * | 2020-12-11 | 2021-04-20 | 江苏大学 | Method for preparing selenium-rich yogurt by taking active nano selenium as selenium source |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1217153A (en) * | 1997-11-12 | 1999-05-26 | 单台阶 | Fresh milk enriched with organic selenium and product thereof |
| CN1251265A (en) * | 1998-10-20 | 2000-04-26 | 北京康必硒生物技术有限公司 | Selenium-rich milk product |
| CN1162090C (en) * | 2000-09-04 | 2004-08-18 | 吴敦虎 | Bioconverted selenium-enriched milk and its production method |
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2003
- 2003-06-20 CN CNB031294391A patent/CN1317964C/en not_active Expired - Fee Related
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN100379346C (en) * | 2005-11-22 | 2008-04-09 | 余内逊 | Method for preparing sour milk contg. micron sugared ginseng, bee-glue etc. for tonifying-Yin and nourishing-stomach |
| CN102138622A (en) * | 2011-01-10 | 2011-08-03 | 苏州硒谷科技有限公司 | Cold drink food rich in selenium substituted amino acid and preparation method thereof |
| CN102613297A (en) * | 2012-04-17 | 2012-08-01 | 光明乳业股份有限公司 | Milk for enhancing organism immunity and preparation process of milk |
| CN102613297B (en) * | 2012-04-17 | 2014-04-09 | 光明乳业股份有限公司 | Milk for enhancing organism immunity and preparation process of milk |
| CN102771563A (en) * | 2012-08-15 | 2012-11-14 | 苏州硒谷科技有限公司 | Selenium-enriched milk product and production method thereof |
| CN104255916A (en) * | 2014-09-18 | 2015-01-07 | 印天寿 | Selenium-enriched yogurt and family preparation method of selenium-enriched yogurt |
| CN105558011A (en) * | 2015-12-16 | 2016-05-11 | 新希望双喜乳业（苏州）有限公司 | Set-style yoghurt capable of enhancing organism immunity and preparation method thereof |
| CN107494731A (en) * | 2017-09-27 | 2017-12-22 | 光明乳业股份有限公司 | A kind of selenium-enriched probioticses acidified milk and preparation method thereof |
| CN107593912A (en) * | 2017-10-13 | 2018-01-19 | 东北农业大学 | A kind of high activity Selenium-enriched fermentation goat milk beverage and preparation method thereof |
| CN112674167A (en) * | 2020-12-11 | 2021-04-20 | 江苏大学 | Method for preparing selenium-rich yogurt by taking active nano selenium as selenium source |
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