CN1579232A - Multi-health-bateria compound nutrient freezed powder capsule and its making method - Google Patents
Multi-health-bateria compound nutrient freezed powder capsule and its making method Download PDFInfo
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Abstract
The invention relates to healthy keeping food, which concretely is a nutrition food based on milk product. According to the design project in the invention, the content of the fungus is not less than 1Xten to power 9 per gram in the frozen dry power capsule, in the alive fungus: Bulgaria fungus: 20-35%; heat indulged streptococcus: 20-35%; bifidobacterium 30-45%; the unit is weight percentage; the invention can make the milk product into capsule, thus it is convenient to be transmitted, carried, reserved and eaten.
Description
Technical field
The present invention relates to health food, specifically a kind of is the nutraceutical that the basis is made with dairy products.
Background technology
Expanding economy and growth in the living standard make people more and more pay close attention to the health of self.This fast lifting and innovation for the fast development of dairy products, quality has brought great opportunity.
Sour milk is subjected to liking of consumers in general with its distinctive nutritive value, yet most of sour milk dairy products are coagulating type, agitating type and beverage type on the market.Their moisture height, big, the easy pollution of volume, storage life weak point have brought inconvenience to the consumer; The vigor quantity of its nourishing healthy value, probio etc. also is subjected to certain restriction; In addition, the preservation of product is also cumbersome with transportation.
Summary of the invention
The objective of the invention is to seek one kind of multiple probio composite nutrient freeze-dried powder capsule and manufacture methods thereof, dairy products can be made capsule according to the method, thereby conveniently transport, carry, preserve and take.
According to design provided by the present invention, in this freeze-dried powder capsule, viable bacteria kind content 〉=1 * 10
9Individual/gram, in the viable bacteria kind, lactobacillus bulgaricus: 20～35%; Streptococcus thermophilus: 20～35%; Bifidobacterium: 30～45%; Unit is a percetage by weight;
At the outside of viable bacteria kind embedding protective agent, form micro-capsule, protective agent consists of: FOS: 15～40%; Oligoisomaltose: 10～25%; Spirulina powder: 1～2%; Composite nutrient: 3～5%; Glycerine 0.5～2%; Moisture: 2～4%, unit is a percetage by weight;
Consisting of of composite nutrient wherein: calcium phosphate: 800～1200mg; Ferrous lactate: 30～80mg; Zinc gluconate: 800～1200mg; Vitamin V
C: 1000～1500mg; Vitamin V
E: 100～200mg; Vitamin V
B1: 10～20mg; Vitamin V
B2: 10～20mg; Folic acid: 1～2mg; Taurine: 5～10mg, unit are per 100 grams.
Method of the present invention comprises: a, one-level inoculation: first class inoculum is inserted in the culture medium, and inoculum concentration is 2～6%, and temperature is 37～43 ℃, cultivates 8～32 hours; B, two-stage inoculation: the bacterial classification after will cultivating through one-level inserts in the fermentation medium again, carries out secondary and cultivates, and inoculum concentration is 2～6%, 37～43 ℃ of temperature, 8～16 hours time; C, detection: utilize the form and the quantity of microscopic examination bacterial classification, detect the vigor of bacterial classification with chemical reagent and pH acidometer; It is characterized in that: d, termination fermentation and precooling: when testing result shows, when the growth curve of bacterial classification reaches the early stage of exponential phase latter stage or stable growth, stop fermentation, then this zymotic fluid is put under 8～12 ℃ the temperature, precooling 10～15 hours induces adaptation for one of the cryogenic freezing of back; E, with protective agent: in above-mentioned zymotic fluid, be added into protective agent,, form micro-capsule with the bacterial classification in the above-mentioned zymotic fluid of protective agent embedding; F, cryogenic freezing: the above-mentioned bacterial classification that is embedded in the micro-capsule placed carry out cryogenic freezing in the freeze drier, when temperature reached 25～30 ℃, freeze drier just entered the vacuum drying program automatically, till making freeze-dried powder.
Again above-mentioned freeze-dried powder is put into the fully-automatic capsule filling and sealing machine, make capsule.
When moisture is many in the zymotic fluid, can before after the precooling,, dewater earlier: the zymotic fluid after the precooling by centrifugal dehydration, is removed wherein redundant moisture, make zymotic fluid be condensed into " bacterium cake " with protective agent.
Described bacterial classification is Bifidobacterium, lactobacillus bulgaricus, streptococcus thermophilus.These bacterial classifications can obtain by purchase.
Described culture medium and fermentation medium are: whole milk powder or skimmed milk power: 12～25%; Soybean or soyabean protein powder: 8～30%; FOS: 2～10%; Oligoisomaltose: 1～8%; Spirulina: 0.5～1%; Sucrose: 8～12%; Glucose: 3～8%; Whey protein concentrate: 4～6%; Cysteine: 0.003～0.008%, remaining for purifying waste water, unit is a percetage by weight.
Described protective agent is: FOS: 15～40%; Oligoisomaltose: 10～25%; Spirulina (dry powder): 1～2%; Composite nutrient: 3～5%; Glycerine 0.5～2%; Moisture: 2～4%; Unit is a percetage by weight.
Consisting of of described composite nutrient: calcium phosphate: 800～1200mg; Ferrous lactate: 30～80mg; Zinc gluconate: 800～1200mg; Vitamin V
C: 1000～1500mg; Vitamin V
E: 100～200mg; Vitamin V
B1: 10～20mg; Vitamin V
B2: 10～20mg; Folic acid: 1～2mg; Taurine: 5～10mg, unit are per 100 grams.
Bifidobacterium, lactobacillus bulgaricus, streptococcus thermophilus mostly are obligate or facultative anaerobe, cultivate so carry out submerged fermentation under the anaerobic state commonly used.What of output the present invention then be according to, keeps behind the medium sterilization inserting through the required bacterial classification after the rejuvenation under its germ-free condition, carries out conventional constant temperature culture (37～43 ℃) in stainless steel cask, fermentation tank.
Environment is made of harmful bacterium and beneficial bacterium in the human body intestinal canal, and beneficial bacterium accounts for 90%, is harmful to bacterium and accounts for 10% usually.Scientific research shows: the drainage of people's physique immunity, the state of mind, complexion facial features and metabolism of himself and vivotoxin is closely related.If often diarrhoea, constipation, this shows his functions of intestines and stomach disorder, and beneficial bacterium is lacked of proper care in enteron aisle.Often diarrhoea causes endotrophic to run off, and causes the generation of malnutritive and other chronic diseases.Often constipation then causes vivotoxin to pile up, and enters blood and infiltrates skin, causes bitter taste halitosis, poor appetite, dysphoria, dim complexion, chloasma, acne, and is heavy then can cause colon cancer.Some fungus, especially antibiotic though can kill the interior harmful germ of enteron aisle, also can have influence on the existence of beneficial bacterium, cause ecological disturbance.So our health need be improved the balance of host enteric microorganism flora especially, probiotic supplemented in time, probio can occupy absolute advantage in the microbial environment so that impel in the enteron aisle.Probio generates nutriment, improves immunity of organisms, suppresses growth of pathogenic bacteria, improves the digestive function of human body, eliminates carcinogen.
High-activity probiotics---Bifidobacterium, lactobacillus bulgaricus, streptococcus thermophilus, biofermentation in the culture medium that milk, soymilk, FOS, oligoisomaltose, spirulina, B B-complex and trace element are formed, probio is bred in a large number, the expansion amount, in its most vigorous stage of vitality, centrifugally concentrate, low temperature (below～30 ℃) freeze-drying, vacuum sublimation form freeze-dried powder.Freeze-dried powder is filled in the hard shell capsules by GMP (i.e. the medicine quality of production management regulation of country's promulgation) production standard under germ-free condition.Especially the protective effect by capsule; the effective active composition of these goods; when taking, can avoid destructions such as hydrochloric acid in gastric juice; enter enteron aisle; improve the microecological balance of human body, the toxin that suppresses and get rid of the parasitic of harmful germ and produced promotes intestines peristalsis; add excitometabolic, fully absorb nutrient.Thereby effectively improve the immunity of human body, and have the health-care effect of antifatigue, adjusting blood fat.
The specific embodiment
In this freeze-dried powder capsule, viable bacteria kind content 〉=1 * 10
9Individual/gram, in the viable bacteria kind, lactobacillus bulgaricus: 20～35%; Streptococcus thermophilus: 20～35%; Bifidobacterium: 30～45%; Unit is a percetage by weight.
At the outside of viable bacteria kind embedding protective agent, form micro-capsule, protective agent consists of: FOS: 15～40%; Oligoisomaltose: 10～25%; Spirulina powder: 1～2%; Composite nutrient: 3～5%; Glycerine 0.5～2%; All the other are moisture: 2～4%, and unit is a percetage by weight;
Consisting of of composite nutrient wherein: calcium phosphate: 800～1200mg; Ferrous lactate: 30～80mg; Zinc gluconate: 800～1200mg; Vitamin V
C: 1000～1500mg; Vitamin V
E: 100～200mg; Vitamin V
B1: 10～20mg; Vitamin V
B2: 10～20mg; Folic acid: 1～2mg; Taurine: 5～10mg, unit are per 100 grams.
The manufacture method of multiple probio composite nutrient freeze-dried powder capsule, comprise a, one-level inoculation: first class inoculum is inserted in the culture medium, and inoculum concentration is 2～6%, and temperature is 37～43 ℃, cultivates 8～32 hours; B, two-stage inoculation: the bacterial classification after will cultivating through one-level inserts in the fermentation medium again, carries out secondary and cultivates, and inoculum concentration is 2～6%, 37～43 ℃ of temperature, 8～16 hours time; C, detection: utilize the form and the quantity of microscopic examination bacterial classification, detect the vigor of bacterial classification with chemical reagent and pH acidometer; D, termination fermentation and precooling: when testing result shows, when the growth curve of bacterial classification reaches the early stage of exponential phase latter stage or stable growth, stop fermentation, then this zymotic fluid is put under 8～12 ℃ the temperature, precooling 10～15 hours induces adaptation for one of the cryogenic freezing of back; E, with protective agent: in above-mentioned zymotic fluid, be added into protective agent,, form micro-capsule with the bacterial classification in the above-mentioned zymotic fluid of protective agent embedding; F, cryogenic freezing: the above-mentioned bacterial classification that is embedded in the micro-capsule placed carry out cryogenic freezing in the freeze drier, when temperature reached 25～30 ℃, freeze drier just entered the vacuum drying program automatically, till making freeze-dried powder.
Again above-mentioned freeze-dried powder is put into the fully-automatic capsule filling and sealing machine, make capsule.
When moisture is many in the zymotic fluid, also can before after the precooling,, dewater: the zymotic fluid after the precooling by centrifugal dehydration, is removed wherein redundant moisture, make zymotic fluid be condensed into " bacterium cake " with protective agent.
Bacterial classification is a Bifidobacterium, and inoculum concentration is 3～6%; And lactobacillus bulgaricus and streptococcus thermophilus, and lactobacillus bulgaricus and streptococcus thermophilus mix with the ratio of 1: 1 or 1: 2, form hybrid bacterial strain, inoculum concentration 2～5%, and unit is a percetage by weight.
Culture medium and fermentation medium are: whole milk powder or skimmed milk power: 12～25%; Soybean or soyabean protein powder: 8～30%; FOS: 2～10%; Oligoisomaltose: 1～8%; Spirulina: 0.5～1%; Sucrose: 8～12%; Glucose: 3～8%; Whey protein concentrate: 4～6%; Cysteine: 0.003～0.008%, remaining for purifying waste water, unit is a percetage by weight.
Protective agent is: FOS: 15～40%; Oligoisomaltose: 10～25%; Spirulina powder: 1～2%; Composite nutrient: 3～5%; Glycerine 0.5～2%; All the other are moisture: 2～4%, and unit is a percetage by weight.
Consisting of of composite nutrient: calcium phosphate: 800～1200mg; Ferrous lactate: 30～80mg; Zinc gluconate: 800～1200mg; Vitamin V
C: 1000～1500mg; Vitamin V
E: 100～200mg; Vitamin V
B1: 10～20mg; Vitamin V
B2: 10～20mg; Folic acid: 1～2mg; Taurine: 5～10mg, unit are per 100 grams.
Below the effect of 3 kinds of probios is narrated, this production day is contained active probiotic 〉=1 * 10
9Individual/gram.
1, Bifidobacterium
Form biological barrier on the intestinal mucosa surface, secrete bifid viable bacteria fork bacillus factor with antibacterial, utilization is supported glycan and is produced SCFA with the stimulation enterocinesia, thereby the generation that activates phagocyte, enhancing igA antibody strengthens immunity of organisms; Can control endotoxemia, the carcinogenic precursor of degrading; A word used in person's names stupid oxygen radical, hydroxy radical and lipid peroxide.Thereby delay senility, can control the synthetic of cholesterol, reduce the generation of angiocardiopathy to reduce the content of serum cholesterol.
Liver is the organ that detoxifies that needs only of human body, and noxious material external or that the human body metabolism produces all will be handled through liver, and via bile or urine discharge, if poisonous substance is too many, will increase the weight of the burden of liver, liver function is obstructed, atrophy, causes the generation of multiple hepatopathy.When beneficial bacteriums such as bifid viable bacteria fork bacillus were preponderated, producing organic acid just can suppress or kill harmful bacterium, reduces the generation of vivotoxin, thereby ensures the health of human body.
2, lactobacillus bulgaricus and streptococcus thermophilus:
These two kinds of bacterium also all are probios, and are the same with bifid viable bacteria fork bacillus, the balance that also can improve, regulate human body enteric microorganism flora, and the immunity of enhancing body reduces cholesterol levels, has the ability of research report assimilating cholesterol can reach 68.5～77.75%.In addition those crowd people's drink milks that lack lactase are easily suffered from diarrhoea afterwards, lactic acid bacteria can be alleviated this crowd's lactose intolerance, thereby promotes the good absorption of dairy products nutrient.Probio is preponderated in vivo, its live body quantity how much be proportional with health.The quantity of probiotics viable bacteria body is no less than 10 in the national regulation acid food
6Individual/gram (ml), but because the self-characteristic of probio and be subjected to the influence of the numerous factors of surrounding environment, cause the product that comes into the market along with the prolongation of holding time, the vigor of probio weakens in the sour milk, and number of viable descends very fast.
The strong body ball of making of the present invention of bifid viable bacteria can provide a large amount of energetic probio quantity reach 〉=10
9Individual/the above microbial inoculum goods of gram (ml), owing to added B B-complex, trace element, especially the bifid viable bacteria fork factor (FOS, oligoisomaltose) has then more fully excited the raising of the immunologic function of human body from the internal cause to the external cause simultaneously.
3, the symbiosis of probio (bifid viable bacteria fork bacillus, lactobacillus bulgaricus, streptococcus thermophilus), these three kinds have the bacterial classification of health-care effect to human body, can symbiosis deposit in our enteron aisle, and can also promote to produce acid, enhancement vigor each other.
Lactobacillus bulgaricus and streptococcus thermophilus symbiosis are fine, experiment show when two kinds of bacterium by (40～45 ℃ of 1: 1 or 2: 1 Mixed culture, 3～4 hours), organic acids such as the lactic acid of its metabolism just can reach 70～96 ° of T, microscope inspection can be seen the thalline that well-grown is sturdy, and quantity can reach 10
8～10Individual/gram (ml).And during single strain fermentation, curdled milk, produce sour vigor and just prolong relatively.Studies show that formic acid that streptococcus thermophilus produces can promote the growth of lactobacillus bulgaricus; And some amino acid peptide class that the lactobacillus metabolism produces etc. can promote the growth of streptococcus thermophilus.
Bifid viable bacteria fork bacillus is when adding Leah lactobacillus, streptococcus thermophilus co-incubation, and the competitiveness of bifid viable bacteria fork bacillus growth is less better.Therefore want the single in addition culture environment (37～40 ℃, composite nutrient) that more adapts to bifid viable bacteria fork bacillus that adopts alone, general require to contain the peptide that protein forms through enzyme hydrolysis or acid hydrolysis, material, especially soyabean oligosaccharides such as several amino acids, vitamin, mineral matter, FOS, oligoisomaltose have good growth promotion breeding effect to bifid viable bacteria fork bacillus.
Select bacterial classification to grow the bacterial strain body in the exponential phase latter stage the most vigorous, that vigor is the strongest, early stage stable growth phase.
The microbial growth process generally can be divided into four stages of development, that is: initial lag phase, exponential phase, stationary phase, decline phase.The present invention pays attention to the vitality of bacterial classification and its survival rate especially in manufacture process.
In order to guarantee that probio can reach best vigor and best number of viable, the condition of culture of selecting good first generation bacterial classification, bacterial strain to be assigned in to adapt to most except that needs, the culture medium prescription, also to pay attention to the growth curve of bacterium simultaneously, monitoring is at any time controlled.Facts have proved that repeatedly when the bacterial cell growth was in exponential phase latter stage or early stage stable growth phase, the bacterial cell vigor was the strongest stage, anti-extraneous factor changes, the metabolism acid producing ability also is in the peak period.The viable bacteria amount of this moment can reach 4.6～8.7 * 10
8Ml
～1More than.
So reach exponential phase latter stage or stable growth preliminary stage termination fermentation in the growth cultivation, transfer 8～12 ℃ precooling stage to, this raising for the making freeze-dried powder technology survival rate of back is very important.
The function of various batchings is as follows:
(1) whole milk powder, skimmed milk power
Whole milk powder, skimmed milk power are the comprehensive dairy products of a kind of nutrient, contain protein 24～26%, contain 20 several amino acids, and wherein casein accounts for 86% of lactoprotein total amount, and lactalbumin accounts for 11%.Contain 1.2% calcium in the casein, forming compound with casein form and calcium phosphate is colloidal state.When leavening reached PH4.6, casein promptly produced precipitation (solidifying) at lactic acid bacteria fermentation process.
Lactose is a distinctive composition in the mammal milk, and belonging to carbohydrate is the required carbon source of lactic acid bacteria, and lactose can be a lactic acid by the lactobacillus-fermented decomposition and inversion.
(2) soya-bean milk or soyabean protein powder
Protein is the basis of life, is the main nutrients that constitutes biological and human body.
Animal protein is mainly derived from foods such as meat, fish, milk, egg in the protein, and animal fat mostly is saturated fatty acid and cholesterol, if it cross the angiocardiopathy that easily causes human body at most.And soybean is to belong to vegetable protein, and is much lower with respect to the milk price.Soybean vegetable protein content can be unrighted acid up to 35～42% its fat, and it does not contain cholesterol, also has the effect that reduces cholesterol level in the blood of human body simultaneously.Amino acid whose composition is also quite complete in the soybean protein, and soybean also contains nutriments such as multivitamin and abundant mineral matter, cellulose in addition.Soyabean oligosaccharides has obtained embodiment to the facilitation of probio growth in our Fermented Soybean Milk fermenting and producing practice process.Soybean protein produces precipitation (solidifying) when the sour environment of PH5.
But soybean has influence on lactic acid bacteria biochemistry for approach because of its lacks lactose during as the culture medium of lactic acid bacteria, and it is more weak to produce acid.If take off raw meat when bad, the local flavor of Fermented Soybean Milk also can be weaker, so we suitably add nutrients such as whey protein concentrate, FOS, oligoisomaltose, spirulina to promote the growth vigor of probio in this culture medium.
(3) FOS, oligoisomaltose
They are the promoter of lactic acid bacteria and bifidobacterium growth propagation, are that a kind of low in calories, low sugariness is not decomposed by saliva and digestive ferment, are not absorbed in stomach, enteron aisle.Lactic acid bacteria especially Bifidobacterium can well utilize it; produce organic acid (cerinic acid, propionic acid, butyric acid, lactic acid), phosphatidic acid and the intestinal epithelial cell effect of cooperatively interacting; occupy the intestinal mucosa surface, formed the biomembrane barrier of one deck protective effect, stop the invasion of pernicious bacteria and grow surely.Organic acid also promote enteron aisle wriggling, suppress or kill the growth of spoilage organisms such as salmonella in the intestines.Studies show that other pernicious bacterias then can not absorb FOS, oligoisomaltose basically in the intestines.
(4) spirulina
Contain rich in protein and reach more than 50%, its nutritive value is very high.1 gram spirulina is equivalent to the comprehensive nutrient content of 1000 gram fruits and vegetables.
Spirullina is in wholefood balanced in nutrition (algae); Contain several amino acids, vitamin and various trace elements; Can eliminate fatigue, recover and activate the brain cell activity, multiple disease, chemotherapy are all had special preventive and therapeutic effect, play the humidification that improves body immunity.Many spirulinas that studies show that can not bring any side effect to human body.
(5) composite nutrient major function:
Calcium phosphate: the calcium source is provided, and is the activator of some enzyme in the body, keeps the normal osmotic pressure of nerve conduction capillary, and acid-base balance in the body also is a calcareous main source in the body.
Ferrous lactate: be the cytochromes composition of hemoglobin and myoglobins, at blood transmission O
2In play an important role, prevent to suffer from anaemia.
Glucose saccharic acid zinc: be the composition of many enzymes and insulin, participate in the metabolism of nucleic acid, protein, other has the appetite of promotion, promotes upgrowth and development of children.
V
C: build up one's resistance to disease, have antioxidation.
V
E: promote appetite and digestion, the neuroprotective tissue is safeguarded heart tissue, also has the effect of mediator's body immunity function.
B
1: be the main component that constitutes apoenzyme, to make full use of carbohydrate essential by health, can improve a poor appetite, and promotes to grow, and prevents neuritis.
B
2: be the main component that constitutes dehydrogenase, promote growth.
B
6: be the coenzyme of various amino acid metabolism enzymes, prevention poor appetite, nauseating, dermatitis.
Folic acid: be the part of DPN, II, antianemia factor promotes digestive function.
Taurine: promote growing of brain, eyesight, body height, and the tool antifatigue effect, the effect that reduces cholesterol is also arranged.
The Bifidobacterium and lactobacillus bulgaricus, the streptococcus thermophilus that breeding strain are in the early stage of exponential phase latter stage or stable growth stop fermentation respectively, be put into 8～12 ℃ then and carried out cold shock 2～4 hours, purpose is to induce adaptation to one of back cryogenic freezing.Carry out sterile working according to GMP production management standard this moment; be added into corresponding bacterial classification protective agent (or further centrifugal dehydration concentrates " bacterium cake "); contain the thalline of part and metabolite and the toxin (lactate) of bacterium in the dehydration liquid; carry out low temperature (25～45 ℃) vacuum freezedrying then, be made into freeze-dried powder.
The principle of vacuum freezedrying is: according to the distillation theory of physics water, be after water in the material freezes, in the following pressure environment of vacuum (610Pa), will can not be melted into water, will directly become the steam dehydration distillation that fades away after be heated (room temperature or 50 following).
When free water in the somatic cells after losing under the frozen state, cells physiological activity, metabolism just stop, and are in resting state, thereby still keep its active material and got off by long preservation.But the cell of thalline also can be subjected to factor affecting such as part freezing injury, dry injury and reduces its viability when the cryogenic freezing vacuum drying.Therefore we will be escorted by protective agent.Keep cyto-architectural stable, suppress to cause in conjunction with water crystallization in the cell membrane the excessively concentrated or ice crystal formation of cytosol in the cell as far as possible.
Protectant kind also is more, and compound such as amino acid, organic acid, low molecular carbohydrate and the glycitols of small-molecular weight arranged; Macromolecular material such as protein, polysaccharide, polyethylene pyrrole alkane ketone and other synthetic polymer or the like are arranged.
Be the comparison of different culture mediums, single bacterial classification or mixed bacteria being cultivated total viable count below.
Culture medium A: whole milk powder, 12%, 125～128 ℃ of sterilizations 4～5 seconds, 8%, 42～43 ℃ of sucrose 8～12 hours;
Culture medium B: whole milk powder, sterilized soyabean protein powder 10%, FOS 5%, 2%, 7～38 ℃ of constant temperature culture of oligoisomaltose 8～20 hours, whey protein concentrate 4% 4～5 seconds for 2%, 25～128 ℃.
Different culture mediums, single bacterial classification or mixed bacteria are cultivated the comparison sheet of total viable count
| B | ??(4.8～6.2)×10 8 | ??(2.2～3.8)×10 8 | ??(6.5～9.2)×10 8 | ??(4.65～7.26)×10 8 |
Viable count is stored the fate comparison sheet at 12 ℃
| Viable count * 10 8 | The 0th day | The 5th day | The 10th day | The 15th day | One month | Three months |
| Lb+St(1∶1) | 46～92 | ?25～38 | ?8～10 | ?4.2～7.8 | ?0.5～1.5 | ?0.03～0.08 |
| Freeze-dried powder (three kinds of bacterium mix) | 42～96 | ?40～96 | ?40～92 | ?40～92 | ?40～89 | ?40～86 |
Freeze-drying or not freeze-drying granulation are with protective agent or do not add protectant viable count comparison sheet
| Freeze-drying viable count * 10 8 | Mist projection granulating viable count * 10 8 | |
| Not with protective agent | 26～30 | 1～3 |
| With protective agent | 42～96 | 3.6～8.5 |
Claims (9)
1, multiple probio composite nutrient freeze-dried powder capsule is characterized in that: in this freeze-dried powder capsule, and viable bacteria kind content 〉=1 * 10
9Individual/gram, in the viable bacteria kind, lactobacillus bulgaricus: 20～35%; Streptococcus thermophilus: 20～35%; Bifidobacterium: 30～45%; Unit is a percetage by weight.
At the outside of viable bacteria kind embedding protective agent, form micro-capsule, protective agent consists of: FOS: 15～40%; Oligoisomaltose: 10～25%; Spirulina powder: 1～2%; Composite nutrient: 3～5%; Glycerine 0.5～2%; Moisture: 2～4%, unit is a percetage by weight;
Consisting of of composite nutrient wherein: calcium phosphate: 800～1200mg; Ferrous lactate: 30～80mg; Zinc gluconate: 800～1200mg; Vitamin V
C: 1000～1500mg; Vitamin V
E: 100～200mg; Vitamin V
B1: 10～20mg; Vitamin V
B2: 10～20mg; Folic acid: 1～2mg; Taurine: 5～10mg, unit are per 100 grams.
2, the manufacture method of multiple probio composite nutrient freeze-dried powder capsule, a, one-level inoculation: first class inoculum is inserted in the culture medium, and inoculum concentration is 2～6%, and temperature is 37～43 ℃, cultivates 8～32 hours; B, two-stage inoculation: the bacterial classification after will cultivating through one-level inserts in the fermentation medium again, carries out secondary and cultivates, and inoculum concentration is 2～6%, 37～43 ℃ of temperature, 8～16 hours time; C, detection: utilize the form and the quantity of microscopic examination bacterial classification, detect the vigor of bacterial classification with chemical reagent and pH acidometer; It is characterized in that: d, termination fermentation and precooling: when testing result shows, when the growth curve of bacterial classification reaches the early stage of exponential phase latter stage or stable growth, stop fermentation, then this zymotic fluid is put under 8～12 ℃ the temperature, precooling 10～15 hours induces adaptation for one of the cryogenic freezing of back; E, with protective agent: in above-mentioned zymotic fluid, be added into protective agent,, form micro-capsule with the bacterial classification in the above-mentioned zymotic fluid of protective agent embedding; F, cryogenic freezing: the above-mentioned bacterial classification that is embedded in the micro-capsule placed carry out cryogenic freezing in the freeze drier, when temperature reached 25～30 ℃, freeze drier just entered the vacuum drying program automatically, till making freeze-dried powder.
3, the manufacture method of multiple probio composite nutrient freeze-dried powder capsule according to claim 1, its feature also is, above-mentioned freeze-dried powder is put into the fully-automatic capsule filling and sealing machine, makes capsule.
4, the manufacture method of multiple probio composite nutrient freeze-dried powder capsule according to claim 1; its feature also is; before after the precooling,, dewater with protective agent: with the zymotic fluid after the precooling by centrifugal dehydration; remove wherein redundant moisture, make zymotic fluid be condensed into " bacterium cake ".
5, the manufacture method of multiple probio composite nutrient freeze-dried powder capsule according to claim 1, its feature is that also described bacterial classification is a Bifidobacterium, inoculum concentration is 3～6%; And lactobacillus bulgaricus and streptococcus thermophilus, and lactobacillus bulgaricus and streptococcus thermophilus mix with the ratio of 1: 1 or 1: 2, form hybrid bacterial strain, inoculum concentration 2～5%, and unit is a percetage by weight.
6, the manufacture method of multiple probio composite nutrient freeze-dried powder capsule according to claim 1, its feature is that also described culture medium and fermentation medium are: whole milk powder or skimmed milk power: 12～25%; Soybean or soyabean protein powder: 8～30%; FOS: 2～10%; Oligoisomaltose: 1～8%; Spirulina: 0.5～1%; Sucrose: 8～12%; Glucose: 3～8%; Whey protein concentrate: 4～6%; Cysteine: 0.003～0.008%; Remaining for purifying waste water, unit is a percetage by weight.
7, the manufacture method of multiple probio composite nutrient freeze-dried powder capsule according to claim 1, its feature is that also described protective agent is: FOS: 15～40%; Oligoisomaltose: 10～25%; Spirulina powder: 1～2%; Composite nutrient: 3～5%; Glycerine 0.5～2%; Moisture: 2～4%, unit is a percetage by weight.
8, the manufacture method of multiple probio composite nutrient freeze-dried powder capsule according to claim 7, its feature also be, the consisting of of described composite nutrient: calcium phosphate: 800～1200mg; Ferrous lactate: 30～80mg; Zinc gluconate: 800～1200mg; Vitamin V
C: 1000～1500mg; Vitamin V
E: 100～200mg; Vitamin V
B1: 10～20mg; Vitamin V
B2: 10～20mg; Folic acid: 1～2mg; Taurine: 5～10mg, unit are per 100 grams.
9, the manufacture method of multiple according to claim 1 or 5 probio composite nutrient freeze-dried powder capsule, its feature also is, according to what of output, keep under its germ-free condition behind the medium sterilization, insert through the required bacterial classification after the rejuvenation, in stainless steel cask or fermentation tank, carry out conventional constant temperature culture.
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| CN101410121B (en) * | 2006-03-29 | 2012-02-29 | 雀巢产品技术援助有限公司 | Dietary supplements containing probiotics |
| CN102613458A (en) * | 2012-04-16 | 2012-08-01 | 广东仙乐制药有限公司 | Probiotics soft capsule and preparation method thereof |
| CN101595976B (en) * | 2008-06-04 | 2012-09-26 | 东北农业大学 | Method for embedding and protecting bifidobacteria by lactalbumin epoxy resin |
| CN102805341A (en) * | 2012-07-11 | 2012-12-05 | 北京阳光一佰生物技术开发有限公司 | Five-time fermentation preparation method of Chinese yam, radix astragali and salviae miltiorrhizae capsule |
| CN103876151A (en) * | 2014-04-09 | 2014-06-25 | 吉林大学 | Probiotic micro-ecological capsule and preparation method thereof |
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| CN101410121B (en) * | 2006-03-29 | 2012-02-29 | 雀巢产品技术援助有限公司 | Dietary supplements containing probiotics |
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| CN102613458B (en) * | 2012-04-16 | 2013-12-18 | 广东仙乐制药有限公司 | Probiotics soft capsule and preparation method thereof |
| CN102613458A (en) * | 2012-04-16 | 2012-08-01 | 广东仙乐制药有限公司 | Probiotics soft capsule and preparation method thereof |
| CN102805341B (en) * | 2012-07-11 | 2014-08-13 | 北京阳光一佰生物技术开发有限公司 | Five-time fermentation preparation method of Chinese yam, radix astragali and salviae miltiorrhizae capsule |
| CN102805341A (en) * | 2012-07-11 | 2012-12-05 | 北京阳光一佰生物技术开发有限公司 | Five-time fermentation preparation method of Chinese yam, radix astragali and salviae miltiorrhizae capsule |
| WO2014148911A1 (en) * | 2013-03-22 | 2014-09-25 | N.V. Nutricia | Fermented nutrition with non-digestible oligosaccharides with increased iron bioavailability |
| WO2014148887A1 (en) * | 2013-03-22 | 2014-09-25 | N.V. Nutricia | Fermented nutrition with non-digestible oligosaccharides with increased iron bioavailability |
| CN105228471A (en) * | 2013-03-22 | 2016-01-06 | N·V·努特里奇亚 | The Nutrious fermented thing with indigestible oligosaccharides that iron bioavailability improves |
| RU2650569C2 (en) * | 2013-03-22 | 2018-04-16 | Н.В. Нютрисиа | Fermented nutrition with non-digestible oligosaccharides with increased iron bio-availability |
| CN103876151A (en) * | 2014-04-09 | 2014-06-25 | 吉林大学 | Probiotic micro-ecological capsule and preparation method thereof |
| CN106085908A (en) * | 2016-06-17 | 2016-11-09 | 王和 | Culture medium of probiotic bacteria polyclone fermentation and preparation method thereof |
| CN108056354A (en) * | 2017-12-27 | 2018-05-22 | 安徽亨博士保健食品有限公司 | A kind of probiotics various nutrients solid beverage and preparation method thereof |
| CN111264870A (en) * | 2020-01-31 | 2020-06-12 | 青岛农业大学 | High-environment-resistance probiotic microcapsule and preparation method thereof |
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