CN1468063A - Products comprising corn oil and corn meal obtained from high oil corn - Google Patents

Abstract

Corn oil and corn meal obtained from high oil corn are included in useful products. The corn oil is extracted from the high oil corn to form the corn meal. The corn oil generally comprises levels of nutrients not found in commercially available corn oils, since most or all of the corn grain, rather than just the germ, is exposed to the extraction process. The corn grain generally includes the steps of flaking corn grain having a total oil content of at least about 6 wt. % and extracting a corn oil from the flaked corn grain. The corn oil is useful for making nutritionally enhanced edible oil or cooking oil, lubricants, biodiesel, fuel, cosmetics and oil-based or oil-containing chemical products. The extracted corn meal is useful for making enhanced animal feed rations, snack food, blended food products, cosmetics, and fermentation broth additive.

Description

Contain by the Semen Maydis oil of high oil corn acquisition and the product of Semen Maydis powder

Invention field

The present invention relates to come free oil-contg to be about the oil that 6wt.% or higher corn extract and the product of powder.

Background of invention

Corn, Zea Mays L., because many former thereby planted, this comprises its application in food and industry.Semen Maydis oil and Semen Maydis powder are derived from two kinds in the useful products of corn.

The commercial refinery that extracts Semen Maydis oil with ordinary method from ordinary maize is divided into its integral part with corn seed, as, endosperm, plumule, terminal bud (tipcap) and epidermis are then from maize germ extracting section Semen Maydis oil.To be the extruding plumule deoil or be with the plumule compressing tablet and use solvent extraction oil to remove the maize germ of producing with wet milling process or dry grinding method.In these two kinds of methods, owing to the remainder of germ separation from corn grain, most of or whole valuable ingredients of endosperm part are not contained in the oil.

The corn-based feeds product that is called as corn feed obtains with the dry grinding method, and it is the mixture of corn bran, maize germ and endosperm, minimum 4% the oil that contains.Make corn feed and need comprise fragmentation, grinding, sieve and be blended in some interior steps, the particle diameter of gained corn feed is less than the particle diameter of the Semen Maydis powder that makes with extraction method described here.

Because the product with the ordinary maize manufacturing lacks some essential nutritive ingredient, industrial and healthy advocate is seeking more nutritious product from corn always.Therefore, people need improved product from Semen Maydis oil and Semen Maydis powder.

Brief summary of the invention

The finished product that contain Semen Maydis oil and/or Semen Maydis powder from ordinary maize comprise, for example, culinary art oil, animal-feed, paper and paper product, various food such as salad cream, compacting and/or expanded dessert, the product that contains maize treacle, corn flakes, thin slice, pudding, cake and bread.

One aspect of the present invention has provided a kind of nutritious animal feed, and it contains from oil-contg and is about 6wt.% or the higher high oil corn Semen Maydis powder remaining behind the extract oil.This animal-feed can also contain other nutritive ingredient, is used in the material in feed supplement field as VITAMIN, mineral substance, high oily seed deutero-powder, digested tankage and bone meal, salt, amino acid, feather meal and many other.Animal feedstuff compositions can be used to special purposes, as poultry feed, pig feed, ox feed, horse feed, aquaculture feed, pet food, and can be used to the growth of animal stage.The special embodiment of animal-feed comprises the chick fodder in the process of growth, the fattening feed of pig and the fattening feed that poultry is laid eggs.Can make feeds product with the Semen Maydis powder that pressed oil, compare with the analogous products that make with ordinary maize, it contains the protein of relative higher proportion and the oil of relatively low ratio.

Embodiments more of the present invention comprise: 1) fibre content of Semen Maydis powder is about 3%, starch content is about 65%, protein content is about 12%, moisture content is about 10%; 2) total oil-contg of high oil corn grain is at least about 6wt.%, is at least about 7wt.%, is at least about 8wt.%; Be at least about 10wt.%; Be at least about 12wt.%; Be at least about 14wt.%; Or from about 7wt.% to about 30wt.%; 3) be complete corn grain or broken corn grain by the corn grain of compressing tablet; 4) corn grain is used to oil expression processes such as solvent extraction, hydraulic pressure or spiral squeezing, the aqueous solution and enzyme extraction; 5) total protein content of high oil corn grain is at least about 7wt.%, is at least about 9wt.%, is at least about 11wt.%, or from the about 20wt.% of about 7%-; 6) total lysine content is at least about 0.15wt.% in the high oil corn grain, is at least about 0.5wt.%, or from the about 2.0wt.% of about 0.25wt.%-; With and/or 7) total tryptophane of high oil corn grain is at least about 0.03wt.%, is at least about 0.20wt.%, or from the about 2.0wt.% of about 0.03wt.%-.

An embodiment preferred provides the method that obtains the Semen Maydis powder (SEC) of Semen Maydis oil and solvent extraction from high oil corn.This method may further comprise the steps: 1) that corn is softening; 2) broken remollescent corn; 3) corn of wetting fragmentation; 4) with wetting corn compressing tablet; 5) extraction is pressed into the corn of thin slice; And 6) from Semen Maydis oil, remove and desolvate and use the solvent extraction Semen Maydis powder.This method provides total content higher Semen Maydis oil, has also increased the protein content in the Semen Maydis powder.In addition, can from SEC, remove the pigment that dissolvable agents is extracted.

Another aspect of the present invention provided a kind of by extracting high oil corn at least endosperm and the Semen Maydis oil base product that contains Semen Maydis oil of plumule gained.This Semen Maydis oil base product can contain other composition, as vinegar, spices, VITAMIN, salt, in order to forming the hydrogen of hydrogenation product, and water.Compare with the product that the Semen Maydis oil that extracts from ordinary maize with ordinary method makes, the Semen Maydis oil that is used in the product of the present invention contains more a high proportion of β-Hu Luobusu, xenthophylls or tocotrienols usually.Be used in Semen Maydis oil in the product of the present invention and normally the corn grain of complete corn grain, broken corn grain or compressing tablet be used to extract and make, and plumule need not be separated from endosperm.Therefore, the nutritive ingredient that dissolvable agents is extracted in the endosperm is extracted and extracts in the Semen Maydis oil of plumule and endosperm.The product of the oil manufacturing that can make with method described herein includes, but is not limited to salad cream, culinary art oil, oleomargarine, spraying food or feed, bread, pancake, dessert, lubricant and fuel.

Other embodiment of the present invention comprises: 1) the high oil corn grain be broken, wetting, compressing tablet and use solvent extraction; 2) total oil-contg of high oil corn grain is at least about 6wt.%, is at least about 7wt.%, is at least about 8wt.%; Be at least about 10wt.%; Be at least about 12wt.%; Be at least about 14wt.%; Or from about 7wt.% to about 30wt.%; 3) Semen Maydis oil is that corn by attrition crushing extracts; 4) Semen Maydis oil is used for solvent base leaching process with the corn grain of compressing tablet and extracts; 5) be used for from the particle of compressing tablet, extracting and comprise any type of commercially available hexane, Virahol, ethanol, supercritical co or their mixture by solvent miscible or alkaline soluble materials; 6) oil of Ti Quing provides with the form of miscella; 7) by other method refined maize oil; And 8) Semen Maydis oil is to be used for hydraulic pressure and/or spiral squeezing, the aqueous solution and/or enzyme extraction method extract by the corn grain with compressing tablet.

A third aspect of the present invention has provided a kind of method of using the Semen Maydis powder that pressed oil in the animal-feed grain ration, comprise: 1) to major general's high oil corn compressing tablet with the corn of making compressing tablet and extract the compressing tablet corn removing wherein a part of Semen Maydis oil, thereby the press for extracting juice Semen Maydis powder of oil excessively is provided; And 2) will press oily Semen Maydis powder is used in the animal-feed grain ration.

A fourth aspect of the present invention has provided the method for using the Semen Maydis oil that extracts in food, comprise: 1) to major general's high oil corn compressing tablet to make the corn of compressing tablet, extraction compressing tablet corn also forms the Semen Maydis oil that extracts to remove wherein a part of Semen Maydis oil, thereby the Semen Maydis oil of extraction is provided; And 2) Semen Maydis oil that extracts is used in the food.

A fifth aspect of the present invention has provided the method for using in the treated oil process as the Semen Maydis oil of the extraction of raw material.This method may further comprise the steps: 1) to major general's high oil corn compressing tablet making the corn of compressing tablet, extraction compressing tablet corn to be removing wherein a part of Semen Maydis oil and to form the crude corn oil of extracting, thereby the crude corn oil of extraction is provided; And 2) crude corn oil of extracting is used for the feedstream of treated oil process.

A sixth aspect of the present invention has provided the method that the powder mix of oil was pressed in various manufacturings.This first embodiment on the one hand provides the method for making the powder mix that pressed oil, comprises the oily excessively powder of press for extracting juice that derives from high oil corn and one or more other oleaginous seed powder.This method may further comprise the steps: 1) high oil corn grain and one or more other oleaginous seed particles are merged the formation granular mixture; And 2) with the granular mixture compressing tablet and be used for extraction process and pressed oily powder mix to remove oil wherein and to form.Second embodiment provides a kind of method that contains following steps: 1) other oleaginous seed with fragmentation and wetting high oil corn and broken and wetting mistake merges to form wetting mixture; 2) with the mixture compressing tablet of wetting mistake to form press sheet mixture; And 3) with the granular mixture compressing tablet and be used for extraction process and pressed oily powder mix to remove oil wherein and to form.The 3rd embodiment provides a kind of method that may further comprise the steps: 1) high oil corn and fragmentation, other oleaginous seed wetting and compressing tablet with broken, wetting and compressing tablet merges to form press sheet mixture; And 2) with the granular mixture compressing tablet and be used for extraction process and pressed oily powder mix to remove oil wherein and to form.The 4th embodiment provides the method that comprises this step: the Semen Maydis powder that will press oil merges to form powder mix with one or more other oleaginous seed powder that pressed oil, and the Semen Maydis powder that wherein pressed oil is to obtain by also extracting to form the Semen Maydis powder that pressed oil to major general's high oil corn compressing tablet.The 5th embodiment provides the blended extracted meal product that makes with above-mentioned any method.

The 7th aspect of the present invention provided the method that the Semen Maydis oil that will extract is used as the beauty treatment composition.This method may further comprise the steps: 1) to major general's high oil corn compressing tablet making the corn of compressing tablet, extraction compressing tablet corn to be removing wherein a part of Semen Maydis oil and to form the crude corn oil of extracting, thereby the crude corn oil of extraction is provided; And 2) crude corn oil of extracting is used for cosmetics.These cosmetics include but not limited to lipstick and eye liner pencil.

Another aspect of the present invention has provided the application of Semen Maydis powder in animal-feed or human food prods, and Semen Maydis powder wherein is to obtain extract Semen Maydis oil from the complete particle of high oil corn after.

Another aspect of the present invention has provided the application of Semen Maydis oil in animal-feed or human food prods, and Semen Maydis oil wherein is to obtain by the complete particle that extracts high oil corn.

Others of the present invention have provided containing Semen Maydis oil and/or containing the product of Semen Maydis powder with method manufacturing described herein.

Except as otherwise noted, all technology used herein have the identical meaning with abbreviation with one of ordinary skill in the art's of the present invention understanding with scientific terminology.Although in practice of the present invention, can use and method described herein and materials similar or method and material of equal value, this and the invention as described herein that do not meant that this type of method and materials limitations.Incorporate all patent applications mentioned and formal analytical procedure in full into here at this, for your guidance.By following to the embodiment of the present invention enumerated and the description of claim, can its its feature of obvious the present invention and advantage.

The accompanying drawing summary

Fig. 1 has shown that the yeast that is grown on yellow dent corn (YD), yellow dent corn powder (YDM), high oil corn (HOC) and the high oil corn powder (HOCM) produces the total amount of ethanol and consumption of glucose.

Fig. 2 has shown the pH value of the yeast culture that contains yellow dent corn (YD), yellow dent corn powder (YDM), high oil corn (HOC) and high oil corn powder (HOCM).

Detailed Description Of The Invention

Known on commercial scale,, and extract corn oil and can effectively from the iblet that contains Chuck Steak, squeeze corn oil rapidly iblet fragmentation wetting and compressing tablet again by randomly. To total oil content of the effective iblet of new compressing tablet oil expression facture greater than about 6wt.%. The raising of iblet oil content can increase the efficient of compressing tablet in the processing procedure. Suitable preforming device and method comprise conventional preforming device and the method that is used for soybean and other similar oily seed compressing tablet. Suitable extraction element and method comprise conventional extraction element and the method for extracting oil of being used for from soybean flakes and other similar oily seed.

The high oil corn seed of any acquisition from several dissimilar milpas or " particle " are useful in the present invention. This type of corn plant can be hybridization, selfing, transfer-gen plant, the plant of genetic modification plant or specific population. Improved high oil corn is used in the oil expression method described herein can makes the improved powder that presses the oil. Useful corn particle type comprises, for example, and jadeite rice, popcorn, powder corn, dent corn, white maize and corn. The high oil corn grain can be any type of, the corn or the corn of other process processing or their part that comprise complete corn, fragmentation, they can compressing tablets but be different from the germ separation standard method of using in dry grinding and wet grindings, so that recovered oil from plumule subsequently.

Terminology used here " whole kernel " or " whole corn " refer to that seed is not divided into each part, and for example, shell, endosperm, terminal bud, pericarp and plumule on purpose are not separated from each other. Whole corn can by or do not ground, crushed, broken, compressing tablet or grinding. The at random separation that a kind of corn composition is not on purpose separately comprised from other composition may be in storage, operation, transportation, crushing, compressing tablet, fragmentation, take place grinding or grinding. Part is on purpose separated certain composition (such as plumule) that refers to major general 50% to be separated from other composition.

Term used herein " high oil corn " refers to contain at least about 6wt.% or higher, is preferably at least about 7wt.% or higher, preferably at least about the iblet of 8wt.% or higher oil. The yellow dent corn that is about 3wt.%-5wt.% with the oil content of routine is compared, and high oil corn has higher oil content. In addition, the total oil content that is fit to iblet of the present invention can be, for example, oil content is at least about 9wt.%, is at least about 11wt.%, is at least about 12wt.%, is at least about 15wt.%, is at least about 18wt.%, is at least about 20wt.%, the particle of the about 20wt.% of about 8wt.%-, the about 30wt.% of about 10wt.%-, the about 30wt.% of about 14wt.%-and these ranges. Although under any moisture, can determine oil content, the oil content in the time of oil content should being corrected to moisture and being about 15.5%.

Described herein can be from Cargill limited company (Minneapolis, the Minnesota State) or Pfister Hybrid Ccorn company (Ai Erpasuo city, Illinois) acquisition to making oil and the useful high oil corn of powder. Other suitable high oil corn comprises the corn variety that is called as Illinois high oil corn (IHO) and Alexandria high oil corn (Alexo), can (University of Illinois Maize Cooperative Stock Center) (Wu Erbanna, Illinois) obtain their sample from illinois university corn gene joint procurement center.

With any iblet that can identify total oil content of raising in the known many methods of the those skilled in the art in this field. Can use American Oil and Chemical Society Official Method, the 5th edition, in March, 1998, (" AOCS method Ba 3-38 ") identifies the oil content of iblet, comprises the fat content of the corn flour that extracts from iblet. AOCS method BA3-38 has carried out quantitatively the material that extracts with benzinum under experimental condition. Oil content or oil concentration are that oil phase is for the percentage by weight of seed sample gross weight. Oil content can be corrected, and under any required moisture condition record.

Here describe other and be used for identifying the appropriate method of high oil corn grain. According to a kind of method wherein, select corncob with the oily detector of near-infrared (NIR), with the corncob of the corn kernel of selecting the oil content that contains raising. Equally, the NIR detector also can be used to select the seed that each has the corn oil level of raising. Yet corncob and/or the seed of the oil content that raising is arranged of selecting can not be differentiated the high oilseeds grain that is fit to processing at an easy rate with method described herein. Usually, plant and gather in the crops the corn seed of the milpa of the iblet that can grow total oil content that can produce raising with known harvesting method. The method of the selfing of development corn, hybridization, transformed variety and population (they have produced the milpa of the iblet of the oil content that can make raising) is known, and be described in Lambert[Specialty Corns, CRC publishing company, the Berkeley village, the Florida State, 123-145 page or leaf (1994)].

A kind of raw material that is used as in the present invention has as shown in Table 1 nutritive peculiarity with the suitable high oil corn of preparation corn oil and corn flour. Numerical value wherein is that the moisture level with " present situation " or " state during charging " represents. Protein, oil and starch level change in being used for the many possible high oil corn combination of the present invention as the raw material use of producing powder and oil. Table 1 has been listed the amount of acceptable moisture, oil, protein, starch, lysine and tryptophan. Yet other combination that does not show as indicatrix in table is such as the protein of 12wt.% and the oil of 12wt.%, also in the scope that can be used to make for the iblet of the present invention's oil and powder.

Table 1.

Composition	Content 1 (wt.%)	Content 2 (2wt.%)	Content 3 (wt.%)	Average content (wt.%)
					Moisture	14	14	14	5-45
Oil	8	12	20	6-30
					Protein	9	9	17	5-20
Starch	61	54	41	35-80
					Lysine	0.35	0.50	1.0	0.15-2.0
Tryptophan	0.088	0.11	0.15	0.03-2.0

What other was suitable has nutritional character as shown in table 2 as raw material to make the iblet that is used for corn oil of the present invention and corn flour. Numerical value wherein is that the moisture level with " present situation " or " state during charging " represents. The numerical value that shows in the table 2 be contain the oil of 12wt.% and 9wt.% the iblet of protein as example.

Table 2.

Composition	Content (wt.%)	Average content (wt.%)
			Moisture	14	5-45
Oil	12	6-30
			Protein	9	5-20
Starch	65	35-80
			Fiber	3	1-5
Ash content	1.18	0.59-4.72
			Lysine	0.33	0.2-2.0
Tryptophan	0.09	0.03-2.0
			Methionine	0.25	0.13-1.00
Total sulfur-containing amino acid	0.46	0.23-1.84
			Valine	0.45	0.23-1.80
Isoleucine	0.34	0.17-1.36
			Arginine	0.45	0.23-1.80
Threonine	0.34	0.17-1.36
			Leucine	1.03	0.52-4.12
Histidine	0.27	0.14-1.08
			Phenylalanine	0.44	0.22-1.76
Alanine	0.70	0.35-2.80
			Aspartic acid	0.74	0.37-2.96
Cysteine	0.22	0.11-0.88
			Glutamic acid	1.9	0.95-7.6
Glycine	0.46	0.23-1.84
			Proline	0.86	0.43-3.44
Tyrosine	0.06	0.03-0.54
			Serine	0.46	0.23-1.84

Table 3 has shown the amino acid levels (be about take moisture 10% iblet be the basis) of two kinds of high oil corn grain samples and common yellow corn grain. Oil and the protein level of high oil corn sample 1 (HOC 1) are respectively 13.3wt.% and 10.7wt.%, and it is take dry as basic representation. Oil and the protein level of high oil corn sample 2 (HOC 2) are respectively 13.0wt.% and 11.2wt.%, and it is take dry as basic representation. In order to compare, take dry as the basis, common yellow corn grain contains the oil of the 4.2wt.% that has an appointment and the protein of about 9.2wt.%.

Table 3.

Amino acid	HOC 1(％)	HOC 2(％)	Yellow corn (%)
				Aspartic acid	0.71	0.68	0.48
Threonine	0.33	0.30	0.19
				Serine	0.37	0.27	0.19

Glutamic acid	1.84	1.79	1.16
				Proline	0.83	0.78	0.52
Glycine	0.40	0.42	0.24
				Alanine	0.77	0.74	0.47
Valine	0.51	0.52	0.33
				Cystine	0.21	0.23	0.16
Methionine	0.46	0.47	0.39
				Isoleucine	0.30	0.30	0.20
Leucine	1.19	1.08	0.74
				Tyrosine	1.11	0.11	0.06
Phenylalanine	0.52	0.47	0.32
				Tryptophan	0.06	0.07	0.05
Lysine	0.34	0.38	0.21
				Histidine	0.29	0.29	0.18
Arginine	0.45	0.48	0.28

High oil corn is normally used for oil expression process described herein so that corn oil and the corn flour that is contained in the raising in the final products of the present invention to be provided. " final products " used herein or " product " term refer to technology or product that corn oil of the present invention and/or corn flour are mixed with various other compositions. Concrete composition contained in the product will be determined by the final use of product. Exemplary product comprises the raw material of food product, edible oil, cooking oil, lubricant, biodiesel, dessert, cosmetics and the sweat of animal feed, chemical modification raw material, biodegradable plastic, mixing. Product comprises food described herein, also comprise the pig, poultry and the ox feed that grow up to or partly grow up to, pet food and people's food is such as the dessert that extrudes, bread and as food adhesive, aquaculture feed, fermented cpds, food additives, sports drink, nutraceutical rod, B B-complex replenishers, weight-reducing drinks and cornflakes food.

For example, from a kind of Types of Maize (for example containing the oil of 12wt.% and the protein of 9wt.%), can make polytype corn flour that can satisfy some nutritional need. This adaptive importance is nutrient density in the feed product and the dietary needs of animal. Use an advantage of such high oil corn and oil expression method to be, according to the degree that oil extracts, the corn flour that presses the oil can be made into specific oil content. Deoil in case from thin slice, remove, the nutrient density of other nutrition that the contained protein of remaining corn flour, amino acid and other are not removed by processing greater than or be different from common iblet, and greater than initial corn, for example, the oil of 12wt.% and the protein of 9wt.% are arranged.

According to a kind of oil expression method of using in making Semen Maydis oil and Semen Maydis powder described herein, complete high oil corn grain can be selected to soften, and selects fragmentation, and then carries out wetting and compressing tablet.Behind the compressing tablet, as described herein, with the corn oil expression of compressing tablet.

Before leaching process, complete corn can randomly be softened.Term used herein " softens " with term " heat soaking " or " steaming " and can exchange, and it is meant and allows the moisture that adds be distributed in whole corn kernel equably.Can use the known softening method in any this field.Usually, corn is soaked in the water of appropriate amount and reaches any suitable time, as is at least 20 minutes, is preferably at least 4 hours, is more preferably at least 12 hours, preferably at least 24 hours.After corn is soaked required time, measure its moisture content.Corn can be preserved by the short period of time, but processed in 24 hours better, preferably processing immediately.

Complete corn grain can randomly be broken.In preferred embodiments, complete high oil corn is in softening back but be broken before wetting.Allow complete corn grain by two rollers that have wave-formed mouth to rotate and leave certain hole round about, and/or by shredder (fluted disc that a rotation is wherein arranged is in the rotation of the place of distance shaft collar certain distance), and/or use hammer mill (wherein the metal of two rotations " hammer " allows device rotation one by one, like this can be with the high oil corn fragmentation.The method of broken corn or high oil corn seed is described in Watson, S.A. ﹠amp; P.E.Ramstad compiles in the Corn:Chemistry and Technology Chapter 11 (1987, AmericanAssociation of Cereal Chemist company, Sao Paulo, the Minnesota State), at this it is incorporated into for your guidance." fragmentation " corn is the corn that has passed through above-mentioned broken processing.

No matter whether corn is broken, can be wetting to it with known method of the those skilled in the art in this field or method described herein.Term used herein " wetting " is meant a kind of course of processing, can make the softening or plasticising of corn kernel so that it is more prone to by compressing tablet and oil expression by this process.Add steam (saturated and/or undersaturated steam) and/or water wetting can comprising in high oil corn.This finishes by using rotary actuator.In the process of adding steam, temperature and moisture all have been enhanced.The scope of temperature is about 140 °F-210 °F, and moisture increases about 1% or about 15%.

Then the high oil corn grain is pressed into various useful sizes.Term used herein " compressing tablet " is meant a kind of course of processing, it allow the corn grain one or many by the compressing tablet rolling mill to make thin slice.Other thickness is about 5/1000-100/1000 inch (about 0.12mm-2.0mm) or is preferably about 0.01 inch (0.25mm) by the final thickness of the corn of compressing tablet, although also can use.Useful sheet thickness depends on outside restrictive parameter, as the oil-contg of corn, moisture content, corn kind (as, dent corn or jadeite rice) and the type of oil press zhai.Here and D.R.Erickson, among the Practical Handbook of Soybean ProcessingUtilization (1995, AOCS press) in detail the suitable method with the high oil corn compressing tablet has been described in detail, it has been incorporated into for your guidance at this.Suitable tabletting method also comprises the known method of the those skilled in the art of oleaginous seed manufacture field.

Be softened at corn, behind broken and/or wetting and the compressing tablet, pressed oily Semen Maydis powder (ECM) so that expressed oil forms thereby press the corn of sheet to be used to milling process.With any oil expression method, by a step or the multistep oil expression step Semen Maydis oil of from the particle of pressing sheet, squeezing out.Usually, just can extract all or nearly all oil with a kind of milling process.Useful oil expression method comprises solvent extraction, solvent extraction continuously, hydrostatic(al) process, spiral squeezing, the aqueous solution and/or enzyme extraction etc.The solvent that effectively is used for solvent extraction comprises, for example, and the commercially available hexane of form of ownership, Virahol, ethanol, supercritical co, their combination or other similar solvent.For example, can use solvent extraction device from the particle of compressing tablet, to extract Semen Maydis oil based on hexane.The solvent extraction device comprises the extractor of infiltration and immersion type.In preferred embodiments, solvent-extraction process allows corn and the solvent thinly sliced keep in touch at least 10 minutes continuously, is preferably at least 30 minutes, is more preferably at least 60 minutes, best at least 90 minutes.

The material of removing from the extractor based on solvent comprises wet thin slice and miscella.Miscella is the oil of proposition and the mixture of solvent.Wet thin slice be with dissolve in the material part of solvent or all extract after the material that is left.Wet thin slice also comprises certain amount of solvent.With climbing-film evaporation or drying, and the method that improves temperature and so on equipment such as the flash tank and/or the device/baking boxs that desolvates reclaims solvent from miscella and wet thin slice.For example, under normal pressure, high pressure or vacuum, wet thin slice or miscella are heated so that solvent evaporation.The solvent that condensation evaporation comes out in isolating recovery system then, and can select to anhydrate, be circulated back in the extractor then.

Remove the miscella of desolvating and typically refer to raw oil, it can be stored and/or continue processing.Raw oil can be refined into final product oil.Refining raw oil is that the those skilled in the art in this field are known with the method that obtains product oil.Hui (1996) provide relevant oil and oleaginous seed detailed summary (Bailey ' s IndustrialOil and Fat Products, the 5th edition, second the volume, Wiley and Sons company, New York, 1996).The chapter 3 of the summary of Hui (the 125-158 page or leaf is incorporated it for your guidance at this) has been narrated the composition and the working method of Semen Maydis oil especially.Described herein is high-quality with the isolating raw oil of pressed disc method, but can continue purifying with conventional olein method for making if necessary.

In preferred embodiments, the present invention relates in the course of processing of high oil corn to reclaim method than light grains (as fine-powder).Term used herein " fine-powder " is meant the normalized definition as ASTM E-11, and any in the corn processed process can be the particle of the #18 sieve of 1.00mm by the aperture.These particulate are reclaimed and can carry out in any stage (as in dewatered process, in broken step or before or after the compressing tablet process) forward and backward in the course of processing or wherein.Usually, fine-powder be by on corn with appropriate speed and direction feed air-flow (as, air, nitrogen, argon gas) with little and light particle band from steam, stay bigger and heavier particle and reclaim.Perhaps, the usefulness liquid spray (as, water, process water) also lighter particle and heavier particle separation can be opened.Liquid is widely used, and it can be used to physics and eliminates lighter, airborne particle.Liquid spray can comprise can increase the composition that the finished product are worth, as VITAMIN, mineral substance, enzyme and their combination.In addition, liquid spray can further comprise caustic liquor.No matter use any separation method, can catch or reclaim these fine particles with known methods in this field such as dust chambers.Preferably, the lighter particle of recovery can be added in the product flow that contains starch to reclaim starch.In addition, this fine-powder also can be used as animal-feed and sells.

Corn endosperm comprises some valuable ingredients, such as carotenoid, xenthophylls, zeaxanthin.Carotenoid in the particle is divided into two and organizes carotene and carotol greatly.Carotene is important, because they are precursors of vitamin A.Blessin etc. [Cereal Chem.40:582-586 (1963)] find that the carotenoid more than 90% mainly is β-Hu Luobusu wherein, is present in the endosperm of yellow dent corn, only is less than 5% and is present in the plumule.Vitamin A is mainly derived by β-Hu Luobusu.

Another kind of valuable ingredients comprises tocotrienols in the endosperm.Grams etc. (1970) find that in corn, tocotrienols only is found, and contains most tocopherol in the plumule in endosperm.Can from plant material, extract tocotrienols with all kinds of SOLVENTS.Lane etc. are at United States Patent (USP) 5,908, have described the method that reclaims tocotrienols from plant material in 940, at this it are incorporated into for your guidance in full.

Therefore, method described herein provides a kind of Semen Maydis oil that is rich in the nutritional fortification of xenthophylls, zeaxanthin and/or β-Hu Luobusu and optional one or more other nutritive ingredients.

Be higher than the similar products like that the Semen Maydis oil that makes in order to ordinary method is made with the amount of the contained important nutritive ingredient of oil-based products of the Semen Maydis oil manufacturing that obtains by extraction method described herein.The Semen Maydis oil that obtains with extraction method described herein comprises Semen Maydis oil and one or more other compositions that extracts from rest of the kernel that obtains from plumule and endosperm.One or more compositions here can be oil, tocotrienols, tocopherol, carotenoid, carotene, carotol and the sterols from endosperm.

Tocopherol (vitamin-E) and vitamin A are antioxidants and are fat-soluble VITAMIN.In the time of in being contained in diet, they are all to healthy and helpful.The oil of oil of the present invention and other or material mixed be deemed to be within the scope of the present invention with β-Hu Luobusu, vitamin-E and the tocotrienols that obtains proper level.In some embodiments, contain the tocopherol of the about 0.5wt.% of 0.1wt.%-that has an appointment by the Semen Maydis oil of the extraction of the description manufacturing here.

The oily contained tocotrienols of making according to method of the present invention can surpass the about 200%-300% of crude corn oil that makes with ordinary method.Extract Semen Maydis oil with optional softening, broken and/or wetting and/or compressing tablet and the method for extracting high oil corn, analyze the content of tocotrienols then.The actual maximum value of tocotrienols content and minimum value will depend on used specific high oil corn.

Hour being that the oxidative stability index (OSI) that unit records is to the tolerance of oil to the relative stability of oxygenizement.Usually, the big more then oil of OSI is not easy oxidized more and makes the time of oily oxidation just long more under test or working conditions.In addition, the content of unsaturated fatty acids is high more in the oil, and OSI is just low more.With extraction method described herein make as the oily common OSI value of example between 10-22 hour.

Blessin[Cereal Chem.39,236-242 (1962); Incorporate into for your guidance in full] describe the method for extracting carotene and zeaxanthin and other pigment in detail.Solvent (mainly being ethanol and hexane) be can be used in combination and carotene and zeaxanthin from corn, extracted.Can use ethanol, hexane, other combination solvent on technical scale, to make oil of the present invention in all proportions.

The exemplary embodiment of the raw oil that obtains with extraction method described herein has the part component characteristic shown in the following table 4 usually.

Table 4

Composition	The high oil corn of exemplary extraction	The high oil corn (scope) that extracts
			FFA (%) C16:0 C18:0 C18:1. is along the suitable anti-C18:3 of C18:2. of the anti-C18:2. of C18:1.	????1.45 ????11.4 ????2.1 ????33 ? ????50 ? ????0.8	????0.7-3.00 ????10-14 ????1.5-3.5 ????26-50 ? ????42-60 ? ????0.6-1.6
Phosphorus (ppm)	????190	????100-400
			Total tocopherol (ppm)	????0.13	????0.1-.50

The general lipid acid of finding in Semen Maydis oil generally includes palmitinic acid, stearic acid, oleic acid, linoleic acid plus linolenic acid.

Can be with the raw oil that method described herein is made by partially or completely hydrogenation.Suitable with oil partially or completely the hydrogenant method be described in D.R.Erickon, Practicle Handbood of Soybean ProcessingUtilization (1995, AOCS press) incorporates it for your guidance in full at this.

When oil-based products constructed in accordance, those products can contain conventional Semen Maydis oil, soya-bean oil, Canadian Semen Brassicae campestris (canola) oil, sweet oil, plam oil, Oleum Helianthi, Thistle oil, antioxidant, seasonings, winterized stearin, partial hydrogenation oil and/or animal tallow.Other oil of the Semen Maydis oil here and one or more mixed just can make the blended oil production.The Semen Maydis oil base product also can contain food supplement, salt, fat, food dye, β-Hu Luobusu, carmine extract, curcumine or turmeric, β-apo-δ '-carotene and its methyl and ethyl ester, natural or synthetic seasonings, antioxidant, propyl gallate, butylated hydroxytoluene, butylated hydroxyanisol, natural or synthetic tocopherol, Quicifal, ascorbyl stearate, dilauryl thiodipropionate, the antioxidant synergistic agent, citric acid, Trisodium Citrate, the sec.-propyl citrate, phosphoric acid, glycerine list citrate, anti-pore forming material, dimethyl polysiloxane, crystallization inhibitor, the hard ester essence of oxidation, amino acid, VITAMIN, mineral substance, carbohydrate, carbohydrate, herbal medicine, spices, acidity regulator, solidifying agent, zymin, the flour treatment agent, viscosity control agent, enzyme, lipid and/or plant or animal proteinum.In addition, can or enrich these edible products with the protein supplements reinforcement that contains available protein.The prescription that can contain picture powder of the present invention, wheat and oatmeal, sugar, salt, maize treacle, Semen Maydis powder, fruit juice concentrate, vitamins C, vitamin B group, folic acid, sodium bicarbonate and seasonings and so on as the exemplary foodstuff products of corn flakes and so on.

Other exemplary oil-based products can contain the oil of preparation here, comprises edible oil, culinary art oil, edible oil and mixing oil.

The equipment of extract oil can be used for making Semen Maydis oil described herein and pressing oily Semen Maydis powder from oleaginous seed (as soybean and Canadian Semen Brassicae campestris).The thin slice that effectively is used for plant-scale oleaginous seed can be from French Oil Mill Machinery company, flat gram, Ohio; Roskamp Champion, Waterloo, Iowa; Buhler, general headquarters are located at Switzerland, in Plymouth, the Minnesota State office are arranged; Bauermeister company, Germany; With Consolidated Process Machinery Roskamp company, World Wide Web Site is http://www.cpmroskamp.com and Crown Iron Works, Minneapolis, and the Minnesota State obtains.

Plant-scale method and apparatus can extract Semen Maydis oil every day at least from about 1 ton of corn.In some embodiments, the ability that technical scale is handled is about 100 tons of corns-Yue 3000 tons of corns every day, or every day about 700 tons of corn-1700 ton corns.Plant-scale operation is handled about corn more than 3000 tons and also be fine every day.

By estimating one or more mass parameters,, can measure the quality of Semen Maydis oil or Semen Maydis powder as oil yield, phosphorus content, free fatty acids per-cent, neutral starch per-cent, protein content and moisture content.Any method can be used for calculating one or more mass parameters to estimate the quality of oil or powder.

Can identify the phosphorus content of raw oil with AOCS method Ca 12-55.AOCS method Ca 12-55 discerns the phosphorus zinc oxide of phosphorus or equivalent, forms the phosphorus of blue phospho-molybdic acid mixture then with metric measurement.AOCS method Ca 12-55 to rough, come unstuck and refined vegetable oils is suitable for.Phosphorus content be multiply by 30 make it change into phospholipids content, be i.e. glue content.In some embodiments, contain the phosphorus of the 100-400ppm that has an appointment by the Semen Maydis oil of manufacturing of the present invention.

Can identify the per-cent of free lipid acid in the oil with AOCS method Ca 5a-40.AOCS method Ca 5a-40 identification is present in the free fatty acids in the oil samples.AOCS method Ca 5a-40 is suitable for all rough and refined vegetable oils, mineral oil and animal tallow.The per-cent of glue and the per-cent of free fatty acids are added the neutral oil that together just can obtain losing in the course of processing.The amount of free lipid acid depends on the amount of the lipid acid of finding in the Semen Maydis oil that extracts in the high oil corn that is used for extracting oil.In some embodiments, the content of free lipid acid is between 0.70wt.%-3.00wt.% in the oil of extraction.

Can determine the color of oil with AOCS method Cc 13a-45.AOCS method Cc 13a-45 is by comparing the color that can determine oil samples with oil samples and known color characteristic.As long as sample is haze-free, AOCS method Cc13a-45 is suitable for fat and oil.Can qualitative evaluation colourity by visual inspection oil.Usually, the result of visual inspection is, compares with known oily color oil content is become light oil and dark oil.Can colourity is quantitative by usefulness AOCS method Cc13a-45 mensuration red value and yellow value.Typically, be about 7-10 with the red value of the conventional isolating crude corn oil of dry grinding method, yellow value is about 60-70.Colourity with the isolating Semen Maydis oil of pressed disc method described herein is more shallow, and is shallower than the crude corn oil that makes with wet milling techniques or dry milling technique usually.Between 60-70, red value is between 7-10 with the determined yellow value of AOCS method Cc 13b-93.

The Semen Maydis oil that extracts can be used as the raw material of chemically modified, the composition of biodegradable plastic, composition, edible oil or oily composition, lubricant or its composition, biofuel or its composition, the composition of snacks, the raw material of fermenting process and the composition of cosmetics of culinary art of mixed food product.Since the oil that obtains with the extraction method here contains one or more from the composition that the non-endosperm of corn kernel partly obtains, this oil just has been modified.In some embodiments, this oil contains 20%-80%, or the oleic acid of 25%-50%, and conventional corn only contains the oleic acid of 25%-40% in oil.When making mixing oil with the oil that extracts, can be before leaching process, central or mix later on.

Can the production biofuel with the Semen Maydis oil that the present invention extracts.Biofuel is the general name of various oxidation dyes based on ester.Nowadays the biofuel of Sheng Chaning is the mixture of the fatty acid methyl ester that makes by refined vegetable oils is methylated.Mainly due to the quality of glycerine byproduct, treated oil is better than raw oil or exhausted frying oil.The main drawback of biofuel product in the past and relevant vegetable oil lubricating agent is cold property, oxidation and polymerisation reactivity.Preferred biofuel product comprises the stearic acid and the poly-unsaturated fatty acid content of low cloud point, minimizing, and high oleic acid content.Yield point is relevant with cold property and be subjected to the influence of saturated fatty acid content in the oil.Easier generation oxidation of poly-unsaturated fatty acids and polyreaction.

When chemical stability was similar, the corn of solvent extraction (SEC) oil had the cloud point performance that is better than soybean.

Table 5

Oil	Palmitinic acid % (16:0)	Stearic acid % (18:0)	Oleic acid % (18:1)	Linolic acid % (18:2)	Linolenic acid % (18:3)	Sinapinic acid % (22:1)
							Vegetable seed	??3	??1	?14	??12	??7	??49
Canada's Semen Brassicae campestris	??4	??1	?60	??20	??9	??2
							Soybean	??8-10	??4	?19-28	??53-56	??6-10	??0
??SEC	??11	??2	?27	??56

Such as can further processing SEC oil corn by the method (seeing that for example, United States Patent (USP) 6,174 No. 501, is quoted at this) of the present announcement of on technology, adopting to form lubricant.

The pruinescence that makes with compressing tablet described herein and oil expression method is used for producing unique plastic prod.Semen Maydis powder used herein is to obtain from complete high oil corn seed after oil expression, and seed wherein is not divided into its integral part, although seed can be ground, compressing tablet, fragmentation, section or grinding, also can not handled like this.Removing the process of deoiling by extraction from corn is to be used for carrying out remaining nutritive substance (as protein and main amino acid) spissated.

Compare with the feeds product that mainly contains the conventional corn grain, the feeds product that mainly contains Semen Maydis powder that makes with extraction method need originate from other (as soybean) add less protein.Because can increase composition by processing, this powder makes the feed-grain maker might produce the feed that can't produce with other method.The Semen Maydis powder that the present invention was pressed oil can make the animal-feed grain ration of specific physical (as bulk density, texture, one-tenth graininess and water holding capacity) and/or special nutrient property as the composition of animal-feed grain ration.Can be used as the Semen Maydis powder of low fat with compressing tablet described herein and the oily excessively Semen Maydis powder of the isolating press for extracting juice of extraction method itself.Perhaps, itself and other Semen Maydis powder or nutritive ingredient can be used in combination to make feed grain ration and food.The Semen Maydis powder that pressed oil also can be used in combination with the powder that makes with soybean, Canadian Semen Brassicae campestris, Sunflower Receptacle, Semen Brassicae campestris, cotton and other crop etc.Also can press the Semen Maydis powder of oil and/or combine with the powder that makes with genetically modified oleaginous seed particle with the corn manufacturing of genetic modification with the powder of formation reinforcement or the product of reinforcement.

Pressed oil Semen Maydis powder can be loose product or the form of particulate product provide, it can randomly combine with other composition.For example, particulate product can comprise by granulating and subsequently by the press for extracting juice of the zein dressing Semen Maydis powder (itself or combine with other composition) of oil excessively.Semen Maydis powder can be contained in in loose or the blended powder-product that particle form provides.

Feed grain ration with the Semen Maydis powder manufacturing of pressing oil generally will satisfy meals and the quality standard that CODEX ALIMENTARIUS or National Research Council work out.The general amount that this powder is listed with table 6 usually contains following composition.

Table 6

??? Composition	??? Sample A??? Content (%)	??? Sample B??? Content (%)	??? Sample C??? Content (%)
				Moisture	????5-45	????5-25	????5-45
Starch	????40-70	????40-80	????40-70
				Protein	????8-20	????7-20	????8-20
Fat (oil)	????0.75-6	????0.75-6.0	????0.75-12
				Robust fibre	????2-4	????2-4
Ash content	????1.5-3	????0.5-2.0
				Fructose	????0.15-0.3
Glucose	????0.2-0.5
				Sucrose	????1.5-2.5
Methionin			????0.2-2.0
				Tryptophane			????0.03-2.0

Above-mentioned Semen Maydis powder can further contain the composition of non-quantitative, does not promptly point out the amount of these compositions.

According to kind, age and method for breeding, different animals need the nutritive ingredient of different levels.High oil corn is carried out in various degree extraction, that is, the high more oil of then removing from corn of the extraction degree of high oil corn is just many more, can make the feed grain ration of the nutritive ingredient that contains different levels.Therefore, by the extraction degree of control high oil corn, can make and contain different fat, protein and the carbohydrate of measuring containing the feed grain ration of Semen Maydis powder that the present invention pressed oil.Table 7 is described content in detail, specified composition measures with this and contains press for extracting juice now excessively in the animal-feed grain ration of Semen Maydis powder of oil, pointed out that the Semen Maydis powder to press oil is the certain content scope of the exemplary grain ration of main component, and pointed out to contain one or more other compositions (as, energy derive based on carbohydrate, as Chinese sorghum, wheat and/or other cereal or their byproduct, or the composition of other non-cereals) the general content range of grain ration.

Table 7

??? Batching	??? General content range	??? Exemplary content range
			Semen Maydis powder described herein	????2-95％	????50-90％
The oleaginous seed powder 1	????3-35％	????10-30％
			Meat and bone meal	????0-12％	????0-7％
Feather meal	????0-6％	????0-4％
			Fat	????0-10％	????1-6％
Salt	????0.1-0.5％	????0.1-0.5％
			Methionin	????0-0.4％	????0-0.4％
Methionine(Met)	????0-0.3％	????0-0.3％
			The nutritive ingredient premix	????0.01-1.0％	????0.01-1.0％

¹The oleaginous seed powder includes, but is not limited to the powder of soybean, Sunflower Receptacle, Canadian Semen Brassicae campestris, cottonseed and other plant base, and they can maybe cannot be used for oily extraction process.

Meat and bone meal are from supplier, as Darling International company (Irving, Texas).The oleaginous seed powder is from supplier, as Cargill Oilseeds (Cedar Rapids, Iowa).Feather meal is from supplier, as Agri Trading company, (Hetchinson, the Minnesota State).Amino acid is from supplier, as DuCoa (Hai Lan, Illinois).

The feed grain ration is by with various materials, as the amino acid of cereal, seed meal, VITAMIN and/or purifying, mix to form a kind of compound substance that satisfies protein, energy, fat, VITAMIN, mineral substance and other nutrient substance dietary requirements amount and make.The blended process comprises and composition ground and mixes to make the mixture of nutritive ingredient relatively uniformly.The physical properties of feedstuff raw material and mixed fodder can influence the integrated value of nutritional status, storage performance and product.The method of suitable manufacturing feed grain ration is disclosed in " feed manufacturing technology IV " (1994, U.S. feed TIA), at this it is quoted in full.

The corn that pressed Semen Maydis powder and steam-flaked (steam-flaked) of oil is like the comparing class with regard to the digestibility of starch part, but owing to the difference of processing conditions, the former has digestibility preferably in ruminating animal.As discussed here, can in the powder that pressed oil, obtain specific oil level by changing processing conditions.Can not reach this press for extracting juice protein, amino acid and the oil level of the powder of oil excessively in steam-flaked ordinary maize, and steam-flaked high oil corn contains more oil, this health to ruminating animal is disadvantageous.

The animal-feed grain ration of many types can press the Semen Maydis powder production of oil with this type, for industrial purposes, following diet type will be described: the powder that makes from corn grain that use (1) here in pork pig fattening diet, the oil-contg of wherein said corn grain is 12wt.%, protein content is 9wt.%, and the oil-contg of the powder that obtains from this corn is 1.5wt.%.(2) powder that makes from corn grain that in poultry chick diet, uses, the oil-contg of wherein said corn grain is 12wt.%, and protein content is 9wt.%, and the oil-contg of the powder that obtains from this corn is 4.0wt.%.

When as the aquaculture feed product, the Semen Maydis powder that the present invention pressed oil has some to be better than the advantage of ordinary maize grain.In agricultural, the pigment of this class of carotenoid often is deposited in the fatty tissue and the color that causes not wishing obtaining in use in the feed.For some fishery products kinds, the tissue that the consumer preference color is more shallow.In other kind, as salmon, consumer preference pink or red tissue.A benefit that the Semen Maydis powder that pressed oil is used for aquatic feeds is, can remove some undesired pigments by the process of making the Semen Maydis powder that pressed oil; The colorant mixture (as carotenoid) that dissolves in solvent is removed from Semen Maydis powder and is concentrated in Semen Maydis oil.Second advantage that the Semen Maydis powder that pressed oil is better than the dry grinding or the corn product of wet-milling is protein content and the quality that improves, thereby this is to make that protein has been concentrated in the powder product owing to removed oil basically from seed.Because this powder comprises major part or whole endosperms from all parts of seed, so compare with the Semen Maydis grit that pressed oil, its protein has higher quality and quantity usually.The Semen Maydis powder that pressed oil is used in and might raises out the less animal of unwanted dye compound in the tissue in the aquatic feeds.

The Semen Maydis powder of solvent extraction also is useful for the production (for example, ethanol, lactic acid and VITAMIN) based on the compound of fermentation.Semen Maydis powder from the solvent extraction of high oil corn can be hydrolyzed so that suitable carbohydrate to be provided.This powder can be used as the carbon source and the energy of bacterium, fungi or yeast culture.Can produce vitamin H and other VITAMIN by culturing micro-organisms.(little) biology comprises variable pseudomonas (ATCC 31014), Corynebacterium primorioxydans (ATCC 31015), arthrobacter, gibberella class, mould mushroom or their combination.

The nutritive ingredient of using in the culture of these and other microorganism comprises, for example, and starch, glucose, alcohol, ketone, and as nitrogenous source, peptone, corn oar, bean powder, ammonium chloride, ammonium sulfate, ammonium nitrate, the press for extracting juice Semen Maydis powder or the urea of oil excessively.Can also contain various salt and trace element in the substratum with culturing micro-organisms.For bacterial species, the pH value of substratum is about 4-9, is preferably to be about 6-8, preferably is about 7.For mould or yeast, pH is about 5-7.In culturing process, temperature remains on 10 ℃-100 ℃, between 20 ℃-80 ℃, is more preferably between 20 ℃-40 ℃ preferably, preferably is about 25 ℃.

United States Patent (USP) 3,859 has been described the production of vitamin H in No. 167, at this it is quoted for your guidance.In the Semen Maydis powder that contains solvent extraction and other suitable microbial species bonded with forming vitamin H are determined the substratum of composition, add suitable-tetrahydrochysene-2-oxygen-4-just-pentane-thieno-[3,4-d] tetrahydroglyoxaline.Usually, microorganism was cultivated 1-10 days, was preferably 1-8 days, was more preferably 2-7 days, after this, vitamin H was separated and purifying.In one embodiment, be the purifying biological element, from substratum, removed cell, absorb filtrate, and use the ion-exchange column purification with activated carbon.Can also be with other purification process, as near iso-electric point, regulating the pH of the solution that contains vitamin H so that its crystallization.

The Semen Maydis powder of solvent extraction also can be further processed to make biodegradable material.For example, powder of the present invention can be contained in the thermoplasticity reagent.Powder of the present invention also can be used on United States Patent (USP) 5,320, in No. 669 described methods, at this it is quoted for your guidance.Thermoplastic material is with the Semen Maydis powder of solvent extraction (as being obtained by working method described herein) manufacturing.In the embodiment, handle with powdered preparation of the present invention with organic solvent and optional linking agent, so that the starch and the protein that pressed in the oily corn grain interconnect.Linking agent refers to connect starch and proteinic any compound here, such as, for example, aldehyde, acid anhydrides or epoxide.So with the composition of powder formation of the present invention can be used to make extrude or biodegradable, the waterproof of mold pressing also/or the article of high-level physical strength are arranged.

The mixing prod that contains the oily excessively Semen Maydis powder of press for extracting juice and one or more other oleaginous seed powder is made by one or more following methods: 1) high oil corn and other oleaginous seed are mixed, fragmentation and/or compressing tablet then are with all seed mixture compressing tablets and be used for leaching process described here to form the blended powder; 2) before high oil corn and other oleaginous seed are mixed with it broken and carry out wetting, compressing tablet and all seed mixtures are used for leaching process described here with formation blended powder then; 3) before high oil corn and other oleaginous seed are mixed with its compressing tablet, and all seed mixtures are used for leaching process described here with formation blended powder; 4) will press oily Semen Maydis powder and press oil or do not press other oily oleaginous seed powder and mix with formation blended powder; Perhaps 5) above-mentioned powder is combined to form the blended powder.Whenever in the above-mentioned course of processing,, in the blended powder, can add added ingredients to make blended product.

The Semen Maydis powder that pressed oil also can be used in picture snacks, mixing foods, bread, fermentation raw material, corn flakes, enriching food (as canned juice), the expanded or food such as ertruding food product and congee.

When being used for the mankind or the edible product of animal, pressed oil Semen Maydis powder can and other powder, other oleaginous seed powder, cereal, other the composition of corn, Chinese sorghum, wheat, wheat ground byproduct, barley, cassava, corn bran powder, corn bran feed, barley byproduct, full-cream rice bran and this class of rice husk of picture mix.

The Semen Maydis powder that pressed oil also can be used as the raw material of producing corn protein isolate, fermentation, further chemical process, and in addition, the enzyme that can also add amylase and proteolytic enzyme and so in powder is to help starch-splitting and protein.

The Semen Maydis powder that pressed oil can randomly be used for the conventional separating starch and the method for protein component.These methods comprise, for example, and dry grinding method, wet milling process, high-pressure pump method or machining at low temperature.These methods and other suitable method are described in Watson, S.A. compile Chapter 11 and 12 chapters (1987 of Corn:Chemistry and Technology with P.E.Ramstad, American Association of Cereal Chemist company, Sao Paulo, the Minnesota State), at this it is quoted for your guidance.Owing to removed the oil in the Semen Maydis powder, compare with the powder that does not extract Semen Maydis oil, pressed in the oily Semen Maydis powder starch and protein component is easier separates with other composition.

The several important mass parameter of pressing the powder of oil comprises fat, starch, protein and moisture content.The method of estimating oleaginous seed opaque amount parameter is described in the AOCS method, at this relevant method is quoted for your guidance.These methods also can be used for the oily excessively Semen Maydis powder of the press for extracting juice of method preparation described herein.

The particulate moisture content can influence compressing tablet processing.The moisture content of corn grain being brought up to about 1%-15% before with the seed compressing tablet is necessary.Make particulate moisture content optimum help abundant processing, this point is that the those skilled in the art in this field are known.

Semen Maydis powder is proofreaied and correct to conventional moisture content, to separating with different methods or comparing at the isolating Semen Maydis powder of different time.The moisture content of oleaginous seed protein concentrates (as Semen Maydis powder or complete corn) is determined with AOCS method Ba 2b-82.The crude fiber content of Semen Maydis powder is determined with AOCS method Ba 6-84.AOCS method Ba 6-84 can be used for particle, powder, flour, feed and but all can therefrom propose the material that contains fiber that fat stays the residual processing thing.The crude protein content of Semen Maydis powder is determined with AOCS method Ba 4e-93.The starch content of Semen Maydis powder is determined with AOCS method Ba 4e-93.The starch content of Semen Maydis powder is to determine with the A-20 method (" corn is made with extra care the A-20 method ") among the Standard Analytical Methods (second edition, on April 15th, 1986) of the member corporation of Corn RefinersAssociation limited-liability company.

Should be appreciated that the analytical method that provides is the example for the effective ways of the various mass parameters of calculating oil described herein and powder here.Other suitable method is known, and they also can be used to calculate describes the also mass parameter of prescription here.

Following examples are used for illustrating specific embodiments of the present invention.The technician who is proficient in this field should be appreciated that the respond well technology that on behalf of the inventor, the technology of pointing out among the following embodiment find is so the example that can be counted as putting into practice in practice of the present invention.Yet for the disclosure herein explanation, the technician who is proficient in this field should be appreciated that, under the situation that does not deviate from spirit and scope of the invention, can carry out many modifications to specific embodiments disclosed herein, so still can obtain the same or similar result.

Embodiment 1

Handle high oil corn with broken, wetting and pressed disc method

This embodiment has described the method that is obtained Semen Maydis oil and Semen Maydis powder by high oil corn.With cylinder distance is that 0.27 inch Roskamp 6.5 series (9 " two) are with the fragmentation of 45 pounds of high oil corn samples.One duplicate samples is used for analyzing, and remaining sample is divided into 4 one's share of expenses for a joint undertaking samples again.Under different temperature (120 °F, 150 °F, 180 °F, 200 °F) carry out every one's share of expenses for a joint undertaking sample wetting respectively.At Crown ^TMHeated sample in 18 inches device/baking boxs that desolvate.When each sample reaches its wetting temperature, make sample pass through sheeter roller.Used sheeter roller is 10 inches of Ross, and the slit is made as 0.007 inch.Take out a thin slice sample, and extract about 500g sample.Wash 4 times each 1200ml hexane wash 20 minutes of using, and spent the 4800ml solvent in 80 minutes altogether.Solvent temperature is about 120 °F.Collect miscella and also pass through the qualitative disk filtration of #4 of diameter 185mm.To filter then the miscella rotary evaporation to estimate oil recovery per-cent.At room temperature with the powder dry air.Take out the sample of oil and powder and analyze their fatty acid profile, starch, protein and fiber.In leaching process, carry out screen analysis and measured the thickness of thin slice.

The miscellaneous equipment that is used for analyzing comprises Mettler Toledo ^TMHR73 halogen water analysis device, OhausExplore ^TMBalance, B ü chi R-114 Roto-Vap ^TM, Crown ^TMExtractor sieve (0.032 sieve) and easily assembling Flex17529-30 type pump.

The color of range estimation raw oil and relatively determines that it is faint yellow with the isolating raw oil of conventional wet milling process (aterrimus).

Characterize the Semen Maydis powder that desolvates with AOCS method Ba 3-38, Ba 2b-82, Ba 6-84 and Ba 4e-93 and corn method for refining A-20.When proofreading and correct when moisture content is 10%, Semen Maydis powder contains 3.2% the fiber of having an appointment, about 65% starch and about 14% protein.Determine that with AOCS method 3-38 the lipid content of powder is about 1.07%.For relatively, with conventional wet milling process make and with the oil-contg of the corn bran feed of moisture content correction to 10% be about 4%, protein content is about 20%, fiber and other carbohydrate content are about 60%.Equally for relatively, proofread and correct to 10% with conventional wet milling process and with moisture content and the corn bran feed of generation can contain 3% the oil of having an appointment, about 60% protein and about 22% fiber and other carbohydrate.

Table 8 has shown the nutritional character of the two kinds of powder (containing 1.5wt.% oil and 4.0wt.% oil) that make with this method.Content is based on that the moisture level of " present situation " or " state during charging " represents.

Table 8

Composition	Powder sample 1 content (%)	Powder sample 2 content (%)
			Moisture	????12	????12
Oil	????1.5	????4
			Protein	????10.5	????10.2
Starch	????58.0	????56.3
			Neutral detergent fiber	????11.3	????11
The acid detergent fiber	????2.8	????2.8
			Ash content	????1.4	????1.3
Methionin	????0.39	????0.37
			Tryptophane	????0.105	????0.102
Methionine(Met)	????0.29	????0.28
			Halfcystine	????0.25	????0.24
Total sulfur-containing amino acid	????0.54	????0.52
			Xie Ansuan	????0.53	????0.51
Isoleucine	????0.40	????0.39
			Arginine	????0.53	????0.51
Threonine	????0.40	????0.39
			Leucine	????1.20	????1.17
Histidine	????0.32	????0.31
			Phenylalanine	????0.51	????0.5
L-Ala	????0.82	????0.79
			Serine	????0.54	????0.52
True metabolizable energy (TMEn; Kcal/kg)	????3023	????3133
			Pig metabolizable energy (ME; Kcal/kg)	????3191	????3301

When the powder that makes with ordinary maize relatively the time, the press for extracting juice described herein Semen Maydis powder of oil excessively provides some crucial nutritive ingredient of higher amount, such as VITAMIN, folic acid, pantothenic acid, Methionin, tryptophane and/or nicotinic acid.For example, powder sample of making as stated above 1 and 2 amounts that show with table 9 contain nutritive ingredient.The amount of same composition and the content in their described here unprocessed yellow corns are compared.

Table 9

Composition	Yellow corn	Powdered sample 1	Powdered sample 2
				Vitamin B6 (mg/100g)	????0.400	???0.820	???0.660
Vitamin B12 (mg/100g)	????0.500	???0.500	???0.500
				Folic acid (μ g/100g)	????-	???25.0	???25.0
Pantothenic acid (mg/100g)	????-	???0.660	???0.890
				Nicotinic acid (mg/100g)	????2.05	???2.30	???1.15

Advantageously, as the press for extracting juice of describe making here excessively the Semen Maydis powder of oil can contain specified level oil and, especially, can contain specific oil: protein, oil: carbohydrate or oil: protein: carbohydrate proportion.For example, conventional corn contains the protein of 8wt.% and the oil of 4wt.%, protein: the oil ratio example is 2.0, and high oil corn contains the protein of 9wt.% and the oil of 12wt.%, protein: the oil ratio example is 0.75.The powder that makes by extraction contains the protein of 10.5wt.% and the oil of 1.5wt.%, protein: the oil ratio example is 7.0.This makes this powder type and be that some application is required with the product that it makes at high proportion, and example is exactly the fattening feed as pig.

The content that the invention provides a kind of xenthophylls, zeaxanthin and β-Hu Luobusu is higher than the Semen Maydis oil from the extraction of the commercially available raw oil of common yellow #2 dent corn.Conventional raw oil can obtain from Cargill company suppliers such as (Minneapolis, the Minnesota States).For example, contain the composition shown in the table 10 in the Semen Maydis oil with the aforesaid method manufacturing, contrast the content that commercially available raw oil has been listed these compositions.

Table 10

Sample	Xenthophylls (mg/g)	Zeaxanthin (mg/g)	β-Hu Luobusu (IN/100g)
				Commercial crude Semen Maydis oil	??0.005	????0.005	??15.5
Oil samples 1	??0.04	????0.012	??72.3
				Oil samples 2	??0.330	????0.112	??302

Embodiment 2

The powder of corn of hanging oneself in the future compressing tablet and extracting processing is as the composition of pork pig fattening feed grain ration

This embodiment describes the comparison of two kinds of DIFFERENT FEED grain rations in detail: first kind contains ordinary maize and contains the press for extracting juice feed grain ration of the Semen Maydis powder of oil excessively without the feed grain ration of solvent extraction and second kind.When thin pork is required finished product, use and contain the press for extracting juice Semen Maydis powder feed grain ration of oil excessively.The content of listing by table 11 with following composition made contain oil-contg less than or the press for extracting juice that the is about 1.5wt.% pork pig fattening feed grain ration of the Semen Maydis powder of oil excessively.This feed grain ration is normally by making composition mediation, mixing and granulating; Yet, when making the feed grain ration, can omit one or more these steps.Table 11 has shown with conventional corn (non-high oil corn) with by containing press for extracting juice that 12wt.% oil, the proteinic high oil corn of 9wt.% the obtains comparison of the pig feed grain ration that makes of the Semen Maydis powder of oil excessively, wherein, press oily Semen Maydis powder and contained have an appointment 1.5wt.% or oil still less (fat).Content is based on that the moisture level of " present situation " or " state during charging " represents.

Table 11

Composition	The fattening of pig feed
			Normal contents (%)	Pressed the Semen Maydis powder (%) of oil
	Corn	????79.98			????-
Pressed the Semen Maydis powder (containing 1.5% oil approximately) of oil			????-	????83.55
	Bean powder	????12.45			????6.60
Meat and bone meal			????6.59	????7.22
	Feather meal	????-			????-
Fat			????0.10	????1.50
	Salt	????0.40			????0.70
Methionin			????0.08	????0.15
	Methionine(Met)	????-			????-
Premix			????0.15	????0.15
	Nutritive ingredient
Crude protein, %			????15.44	????15.78
	???ME，kcal/kg	????3200			????3200
Robust fibre, %			????1.96	????2.12
	Calcium, %	????0.85			????0.85
Phosphorus, %			????0.58	????0.58
	Amino acid, %
Arginine			????0.96	????0.93
	Halfcystine	????0.28			????0.29
Histidine			????0.40	????0.42
	Isoleucine	????0.57			????0.58
Leucine			????1.39	????1.49
	Methionin	????0.81			????0.81
Methionine(Met)			????0.26	????0.34
	Phenylalanine	????0.70			????0.72
Threonine			????0.56	????0.58
	Tryptophane	????0.14			????0.14
Tyrosine			????0.47	????0.48
	Xie Ansuan	????0.72			????0.75

In table 11, provided the absolute value of composition percentage composition, yet, in fact, in the composition of the listed inclusion ratio of other form is also included within the use literary composition.

Some advantages of this new feed grain ration are, the user of powder does not need corn is ground, therefore can save the energy intensive step, for satisfying the less soybean of required protein level needs or other oleaginous seed powder, and this powder has the digestibility that is better than corn grain.

Embodiment 3

The powder of corn of hanging oneself in the future compressing tablet and extracting processing is as the composition of poultry fattening feed grain ration

This feed grain ration is used to satisfy the high energy demand of the birds (as chick) in the growth.The content of listing by table 12 with following composition made contain oil (fat) content less than or the press for extracting juice that the is about 4wt.% chick fattening feed grain ration of the poultry of the Semen Maydis powder of oil excessively.This feed grain ration is normally by making composition mediation, mixing and granulating; Yet, when making the feed grain ration, can omit one or more these steps.

Table 12 has shown with conventional corn (non-high oil corn) with by containing press for extracting juice that 12wt.% oil, the proteinic high oil corn of 9wt.% the obtains comparison of the pig feed grain ration that makes of the Semen Maydis powder of oil excessively, wherein, press oily Semen Maydis powder and contained have an appointment 4wt.% or oil still less (fat).Content is based on the absolute value representation of the moisture level of " present situation " or " state during charging " and the composition percentage ratio of giving, yet, in the practice, in the composition of the listed inclusion ratio of other form is also included within the use literary composition.

Table 12

Composition	Chick in the growth
			Normal contents (%)	Pressed the Semen Maydis powder (%) of oil
	Normal corn	????66.85			????-
Pressed the Semen Maydis powder (containing 4% oil approximately) of oil			????-	????70.86
	Bean powder	????20.96			????16.42
Meat and bone meal			????5.00	????5.00
	Feather meal	????2.00			????2.00
Fat			????3.29	????3.76
	Salt	????0.37			????0.37
Methionin			????0.13	????0.19
	Methionine(Met)	????0.15			????0.09
Premix			????0.10	????0.10
	Nutritive ingredient
Crude protein, %			????19.48	????19.52
	?ME，kcal/kg	????3100			????3100
Robust fibre, %			????1.97	????2.12
	Calcium, %	????0.94			????0.94
Phosphorus, %			????0.63	????0.62
	Amino acid, %
Arginine			????1.27	????1.23
	Halfcystine	????0.38			????0.39
Histidine			????0.47	????0.48
	Isoleucine	????0.78			????0.79
Leucine			????1.68	????1.74
	Methionin	????1.06			????1.06

Methionine(Met)	????0.44	????0.44
			Phenylalanine	????0.92	????0.92
Threonine	????0.74	????0.75
			Tryptophane	????0.19	????0.20
Tyrosine	????0.61	????0.62
			Xie Ansuan	????0.95	????0.96

Embodiment 4

The oil of corn of hanging oneself in the future compressing tablet and extracting processing is as food ingredient, or as the raw material of purifying seed composition

Among this embodiment, the oil of tocotrienols content above the about 200%-300% of crude corn oil that makes with ordinary method has been described.With compressing tablet and the extracting method of embodiment 1, from being about the high oil corn of 12wt.%, oil-contg extracts Semen Maydis oil.Analyze the tocotrienols content of Semen Maydis oil then.Following table comprises the α of the extraction Semen Maydis oil that makes with the ordinary maize oil that is made by ordinary maize with ordinary method with according to the method for embodiment 1-and γ-relevant data of tocotrienols content.Conventional raw oil is meant unpurified Semen Maydis oil sample.This sample has been represented the usual production of most of present manufacturings.As described below, it is found that, the extraction that under 120-200, from two kinds of different high oil corn samples, extracts with solvent from the tocotrienols content of oil (EWKO) sample of complete seed approximately be conventional raw oil sample 2-3 doubly.The tocotrienols content of EWKO sample is between γ-tocotrienols of the alpha-tocotrienol of 26ppm-33ppm and about 48ppm-84ppm.Usually, the content that temperature will improve tocotrienols in the Semen Maydis oil that extracts is extracted in rising.The minimum value and the maximum value of actual tocotrienols content depend on used specific high oil corn.

Table 13

Sample	α tocotrienols (ppm)	γ tocotrienols (ppm)
			Conventional raw oil (contrast)	???11.88	???29.94
????EWKO?1?120-200F	???29.36-33.19	???48.11-59.36
			????EWKO?2?120F	???26.05-28.43	???79.55-84.21

Therefore, the method for embodiment 1 is used to make the Semen Maydis oil of the extraction of the tocotrienols level that contains raising.

Embodiment 5

The powder of corn of hanging oneself in the future compressing tablet and extracting processing is as the composition of the mixed animal feed product that contains Semen Maydis powder and oleaginous seed powder

This embodiment has told about a kind of new forage component that contains the mixture of Semen Maydis powder that useful compressing tablet and oily extraction method make and another kind of plant-derived powder (as the oleaginous seed powder).This blended material can only be the loose accumulative mixture of these two kinds of powder, also can be granular product.Because the method for corn and oleaginous seed powder of making is similarly, that is, broken, wetting, compressing tablet and solvent extraction are so can produce these two kinds of powder together and before selling the human consumer they are mixed.An advantage of doing like this is to realize the protein and the energy of various levels with a kind of powder.At powder mixes stage or the adding added ingredients that can choose wantonly in terminal stage.For example, the energy-intensive step in the feed manufacturing is included in and grinds corn grain in the feed grinder and it is mixed with other composition.Compare with the powder mix of routine, powder mix of the present invention needs less energy to make final feeds product usually.

The nutrient substance feature that table 14 shown bean powder (SBM), pressed the nutrient demand of the mixture (S10-C90) of mixture (S20-C80), 10%SBM and 90%ECM of Semen Maydis powder (ECM), 20%SBM and 80%ECM of oil and poultry and pig diet.The nutrient demand of poultry and pig is to list according to the guide of National Research Council (NRC).ECM makes according to embodiment 1.

Table 14

Parameter	?SBM	?ECM	20% SBM and 80% ECM	Poultry nutritional needs every day	10% SBM and 90% ECM	Pig nutritional needs every day
							Crude protein (CP)	?47.5	?10.2	??17.66	??18	????13.93	????13.2
Pig ME, kcal/kg	?3380	?3301	??3316.8		????3308.90	????3265
							Poultry ME, kcal/kg	?2440	?3133	??2994.4	??3200	????3063.70
Crude fat, %	?3	?4	??3.8		????3.90
							Neutral detergent fiber, %	?8.9	?11.3	??10.82		????11.06
The acid detergent fiber, %	?5.4	?2.8	??3.32		????3.06
							Arginine	?3.48	?0.45	??1.06	??1.00	????0.75	????0.19
Histidine	?1.28	?0.27	??0.47	??0.27	????0.37	????0.19
							Isoleucine	?2.16	?0.34	??0.70	??0.62	????0.52	????0.33
Leucine	?3.66	?1.03	??1.56	??0.93	????1.29	????0.54
							Methionin	?3.02	?0.33	??0.87	??0.85	????0.60	????0.60
Methionine(Met)	?0.67	?0.25	??0.33	??0.32	????0.29	????0.16
							Halfcystine	?0.74	?0.21	??0.32	??0.28	????0.26	????0.35
Phenylalanine	?2.39	?0.44	??0.83	??0.56	????0.64	????0.34
							Tyrosine	?1.82	?0.29	??0.60	??0.48	????0.44	????0.55
Threonine	?1.85	?0.34	??0.64	??0.68	????0.49	????0.41
							Tryptophane	?0.65	?0.09	??0.20	??0.16	????0.15	????0.11
Xie Ansuan	?2.27	?0.45	??0.81	??0.70	????0.63	????0.40
							Total indispensable amino acid (EAA)	?23.99	?4.49	??8.39	??6.85	????6.44	????4.17
??EAA/CP	?0.505	?0.440	??0.45	??0.381	????0.45	????0.316

Embodiment 6

Process high oil corn with pressed disc method

Use Perten ^TM(model: 9100-H.F), PertenInstruments (Reno, the state of Nevada) screens the band shell seed of total oil-contg greater than yellow each fringe of dent corn of about 7wt.% volume near infrared (NIR) seed-test machine (TM).At Brimrose ^TMIn the seed expert single grain NIR trier (Brimrose company, Baltimore, the Maryland State) further the screening oil-contg be at least the seed of the fringe of 7wt.%, be at least indivedual seeds of 13% to select oil-contg.Seed is kept at moisture content to be about under 13.5% the condition.When adding man-hour, the moisture content of seed is about 10%.

With comprising that one two inches stainless steel bar and dull and stereotyped desk-top preforming device are with complete corn grain compressing tablet.Allow complete corn grain compressing tablet by cylinder four times so that final sheet thickness is about 0.01 inch.N-hexane and Kimble with (60-℃-65 ℃) of heat ^TM585050 type Soxhlet extractors extract miscella from the corn grain of compressing tablet.Gained miscella and Semen Maydis powder are desolvated.Heating miscella is desolvated to remove under the vacuum of 25 inches mercurys.According to AOCS method Ba 2a-38 Semen Maydis powder is desolvated.

The oil of all recovery accounts for all corn grain sample 14.74wt.%.The phosphorus content 365ppm of the raw oil of measuring with AOCS method Ca 12-55 that desolvates.Phospholipid concentration is defined as 1.095% (0.0365%*30).The free fatty acid content of measuring with AOCS method Ca5a-40 is 0.2%.The neutral oil that loses in the course of processing is 1.3% (1.095%+0.2%).Use the same method, the phosphorus content that extracts the raw oil of certainly conventional (being that total oil-contg is 3-4wt.%) corn grain with the wet milling process of routine is about 600ppm-800ppm, free fatty acid content is about 0.5%-1.0%, and the neutral oil that loses in the course of processing is about 3%-4%.

The color of range estimation raw oil and with the isolating raw oil of conventional wet milling process (aterrimus) comparison, determines that it is faint yellow.

Characterize the Semen Maydis powder that desolvates with AOCS method Ba 3-38, Ba 2b-82, Ba 6-84 and Ba 4e-93 and corn method for refining A-20.When proofreading and correct when moisture content is 10%, Semen Maydis powder contains 3.2% the fiber of having an appointment, about 65% starch and about 14% protein.Determine that with AOCS method 3-38 the lipid content of powder is about 1.07%.For relatively, with conventional wet milling process make and with the oil-contg of the corn bran feed of moisture content correction to 10% be about 4%, protein content is about 20%, fiber and other carbohydrate content are about 60%.Equally for relatively, proofread and correct to 10% with conventional wet milling process and with moisture content and the corn bran feed of generation can contain that 3% the oil of having an appointment contains, about 60% protein and about 22% fiber and other carbohydrate.

Embodiment 7

The method of refining high oil corn

This embodiment has described continuous solvent-extraction process hereinafter.This extraction method consists essentially of four parts: preextraction, extraction, powder desolvate and oil desolvates.Below will be described these different stages in more detail.

(A) preextraction

5.4 tons of complete high oil corns (oil that 12wt.% is arranged approximately) seed is softening, then material is delivered to crusher from the door of hopper through chain bucket elevator.By crusher, particle (that is, the particle of complete corn) is sent in the setter, and setter is delivered to material in the isolated haulage system.This system comprises second chain bucket elevator, aero-mechanical transfer roller, hot steam cover transfer roller and the skewed slot that is connected in series.By haulage system, the corn particle is supplied to the compressing tablet roller.

Deliver to before the crusher, at 350 liters Toronto Coppersmithing ^TMIn the corn of " present situation " humidity, add water in the Toreo R-12 type ribbon formula stirrer so that complete corn softens the moisture content to 14.5%.Speed with 2 liters/hour is sprinkled water in container.Behind the water that adds appropriate amount, again corn was stirred 1 hour.Before measuring its moisture content with corn soaking 24 hours.Then the remollescent corn is stored 11-15 days.

After the storage, at room temperature use Roskamp ^TM(Waterloo, Iowa) 6.5 serial model No.s, have diameter 9 " length 12 " the broken roller of double bracket of roller is the fragmentation of remollescent corn.The roller of top and bottom all is arranged to a roller to be changeed sooner than another roller.Fast roller per minute changes 1065 and changes (rpm), and per inch has 6 spiral RBV cutting ripples.Slow roller equally cuts but rotates with the speed of 708rpm.Broken humidity is 13.3%-15.7%.Produced the particle of following average particle size distribution scope: 15.9% is held back by U.S.'s #4 mesh sieve, 39.9% is held back by U.S.'s #6 mesh sieve, 27.8% is held back by U.S.'s #8 mesh sieve, and 6.8% is held back by U.S.'s #10 mesh sieve, and 4.3% is held back by U.S.'s #18 mesh sieve and 5.3% held back by U.S.'s #18 mesh sieve.

(Simon-Rosedowns belongs to De Smet now having being known as of rotary cleaning arm the double-deck setter of double centner working ability is arranged then; Prins Boudewi jnlaan 265; B-2650 EDEGEM; Antwerp) carry out the corn of fragmentation wetting in (every layer diameter 36 inches, high 20 inches).The bottom oepration at full load.In the residence time in uperize stage is 55 minutes.Regulate the broken degree of depth of top layer so that be 94 minutes in residence time average out to 39 minutes, total residence time of direct heating part.Speed uperize with 0-5kg/hr.The wetting humidity that withdraws from is 12.1%-14.5%.Withdrawing from temperature is 75 ℃-85 ℃.

Use Roskamp (Waterloo, Iowa) 2862 type tabletting machines that the corn of fragmentation is laminated then.Tabletting machine has 62 inches long 28 inches wide rollers.Agent set is configured to make fast roller to rotate with 300rpm, and it is 8% that internal trolley drives (IRD) rate.Roller pressure is remained on 500psig.The humidity that withdraws from of compressing tablet is 9.1%-11.7%, and withdrawing from temperature is 60 ℃-83 ℃.The thickness of thin slice is between 0.3mm-0.7mm, and 0.2mm (0.008 inch) preferably is arranged in the slit of roller.

(B) extract

Crown with successive 150kg/hr ^TM(Roseville, California) II type pilot scale extractor (pilot extractor) processing is pressed into the corn of thin slice.As solvent, it has 5 reverse miscella washing sections and an afterbody washing part to the extractor of this pilot scale with the blended hexane.The fresh hexane of 6 miscella recirculating pumps and 50 ℃-60 ℃ is installed to together the top of extractor.The size of extractor is that 29 feet long, 7.8 inches wide and 4.5 feet are dark.With 23 feet in 29 feet extractors wetting, wherein 19.5 feet are washed.Average process velocity is about 75kg/hr.The residence time is about 60 minutes.The ratio of solvent and powder is transferred between 0.75: 1 to 1.33: 1.All miscella is delivered to 27 ℃-34 ℃ oil desolvates in the device.

(C) powder desolvates

At first at Schnecken ^TMCarry out the periphery in (Crown Iron Works, Roseville, California) steam sleeve transfer roller (SJC) and non-direct heating desolvates.SJC contains free scraper plate screw rod in steam sleeve (12 feet long, 10 feet of diameters).The materials conveyance that to press oil when the scraper plate screw rod of opening is when crossing transfer roller, and it produces rotary movement, thereby guarantees that all materials all are exposed to hot wall.Gas pressure regulator is regulated the quantity of steam of supplying with chuck.Regulate the temperature in transfer roller exit, and it is supplied with the basis of the steam of chuck as control.In the rough vacuum condenser, collect from the steam of transfer roller by the little negative pressure that produces by system fan.Use one to have the rotary cleaning arm and be known as the bilayer that the 100kg working ability is arranged desolvate device and baking box (DT) (36 inches of diameters, 20 inches of every floor heights).Steam only sprays by the upper strata cleaning arm.The humidity that withdraws from of powder is 9.4%-17.7%, and withdrawing from temperature is 57 ℃-104 ℃.The hexane that reclaims from SJC is condensed, dewaters and circulates in extractor.

(D) oil desolvates

Oil desolvates and carries out with rising-film evaporator (RFE).This device contains the test tube of 16 diameter 1.5cm in a big chuck.Be full of steam in the chuck with the heating test tube.The loaded liquid of extracting (the conventional oil in the hexane is become miscella) is installed to the test tube bottom.When it in vitro raise, the heat of steam made liquid boiling.The steam that has liquid leaves the film that tube wall arrives the thin rise of one deck.At the top, liquid is separated with steam.Oil flows to upflow tube and arrives oil wiper (OS), and steam is brought in the condenser.Test tube is placed under the vacuum, so liquid seethes with excitement at low temperatures.

Oil wiper is a dish and cyclic distillation column.Liquid spreads out and drips in the film in dish in the ring at dish back, and this makes oil in the post cascade.Simultaneously, feed steam in the bottom of oil wiper, steam passes through liquid, thereby removes the solvent that is retained in the liquid.The steam sleeve of promising liquid and steam insulation around dish and ring-type post.Oil wiper also can be operated under vacuum.The hexane that reclaims from rising-film evaporator and OS is condensed, dewaters and recirculation in extractor.

(E) analyze the oil that obtains by high oil corn

With recovery of oil and analyze its VITAMIN, lipid acid and trace element.In contrast, use the same method and extract the yellow #2 corn of 800lbs., analyze with recovery of oil and to same composition simultaneously.Vitamin A and β-Hu Luobusu are analyzed with patented method in contract laboratory (contract lab).The process of other announcement comprises Bates etc., Proc.Fla.State Hort Soc., 88,266-271 (1975).Go up with CP88 cyanogen propyl group post (100m * 0.265mm in gas-chromatography (GC), 0.5mm thickness) and flame ionic detector press American OilChemist Society (AOCS) method Ce 1c-82, Ce 2-65, Cd 3a-94 and Cd 1c-85 analyze free fatty acids.

At high performance liquid chromatography (HPLC, the Waters2590 type) upward analyzes tocopherol and tocotrienols as moving phase with conventional silica column and with hexane-Virahol, and, detect according to the method for describing among the AOCSCe 8-89 with fluorimetric detector (Waters 2690 types).Be moving phase analysis xenthophylls with the C30 reversed-phase column with water-acetonitrile and detect by HPLC with the UV detector.

Table 15, as follows, listed comparison by the oil component of high oil corn and yellow #2 corn gained.For relatively, give the composition that extracts with the corn wet milling method from the oil of yellow #2 corn.

Table 15

Composition	High oil corn	Yellow #2	Yellow #2, wet milling of corn
				Palmitinic acid %	????11.4	????10.7	??10.7
Stearic acid %	????2.2	????1.9	??2.0
				Oleic acid %	????35.6	????25.5	??27.5
Linolic acid %	????48	????58.4	??57.1
				Linolenic acid %	????0.7	????1.2	??1.1
Alpha-tocotrienol (ppm)	????184	????48	??12
				Alpha-tocopherol (ppm)	????237	????231	??136
VITMAIN B1, mg/100g	????0.390	????NA	??0.260
				Wei ShengsuB2, mg/100g	????0.090	????NA	??0.080
Vitamin B6, mg/100g	????0.82	????NA	??0.4
				Vitamin B12, mg/100g	????0.5	????NA	??0.5

Embodiment 8

In the dehumidifying step, reclaim lighter particle

This embodiment has listed a kind of method than light grains (as fine-powder) that produces in the dehumidifying step that is recovered in from the course of processing of high oil corn.

As described in embodiment 7, high oil corn is broken and compressing tablet.The complete corn that is pressed into thin slice by compressing tablet process gained is heated to use the standard processing units, as Kice SSI A2612 type zigzag separator, removes moisture.In the dehumidifying step, the air-flow that control can be regulated so that less and lighter particle can be taken out of, separates they and heavier thin slice thus.The example of this air-flow that can control is Crown ^TMMultistage suction system, its per minute can be handled 2600 cubic feet.Standard treatment unit with dust chamber and so on reclaims lighter particle.The lighter particle that reclaims is added in the product flow that contains starch to reclaim starch.

Embodiment 9

In broken step, reclaim method than light grains with air

This embodiment has listed a kind of method than light grains (such as fine-powder) that produces in the broken step that is recovered in from the course of processing of high oil corn.

With the crusher roller of standard such as Roskamp 6.5 series (Waterloo, Iowa) complete seed damage with high oil corn.In broken step, allow the air communication that can regulate cross the crusher roller, and regulate air-flow speed so that less and lighter particle along with air-flow is taken out of, they and heavier particle can be separated thus.The example of this air-flow that can control is Crown ^TMMultistage suction system, its per minute can be handled 2600 cubic feet.Standard treatment unit with dust chamber and so on reclaims lighter particle.The lighter particle that reclaims is added in the product flow that contains starch to reclaim starch.

Embodiment 10

With the method for liquid spray recovery than light grains

This embodiment has listed and has been recovered in before the compressing tablet process and the method for the fine-powder that produces afterwards with liquid spray.

As described in embodiment 7, high oil corn is broken and compressing tablet.The corn of the fragmentation before the compressing tablet and the cornflakes after the compressing tablet sprayed or cage in the liquid of enough large coverages is arranged so that remove lighter airborne particle physically.Water can be used as this liquid.Perhaps, liquid spray can be the material that can increase the value of the finished product and recover the value of fine-powder.Liquid spray is pure water, process water or added the water of nutritional additives such as VITAMIN, enzyme or mineral substance normally.Under all situations, contain the particulate liquid flow all by band from heavier particle and be collected.With the standard processing units separating particles from liquid that comprises wet cyclone or whizzer.At random, the fine-powder of recovery can be dried and use for continuing.The lighter particle that reclaims is added in the product flow that contains starch to reclaim starch.

Embodiment 11

The food of mold pressing

This embodiment has described the Biodegradable material that improved tensile strength is arranged with the oily excessively Semen Maydis powder manufacturing of press for extracting juice of the present invention.

Semen Maydis powder with 2: 3: the ratio of solvent is suspended in Semen Maydis powder of the present invention in the hexane in the encloses container.Allow mixture at room temperature leave standstill 18 hours, do not stir.Remove organic solvent from the Semen Maydis powder that pressed oil, use resistates in filtration procedure: the weight ratio of solvent is the Semen Maydis powder resistates that 1: 1 part hexane wash is extracted.In the stove of routine under 50 ℃ with dry 16 hours of resistates.The moisture content of spilling water and stir up to resistates on the exsiccant resistates reaches 10.7%-11.3%.With compression molding press (Wabash MetalProducts company, Wa Baishi, the Indiana), will press 10 minutes with the oily excessively Semen Maydis powder composition of press for extracting juice that solvent treatment is crossed down, be molded into ASTM standard dog-bone product (dogbone) for 140 ℃-160 ℃ at 5000psi.Untreated Semen Maydis powder composition may be combined with the water of 10.7%-11.3%, and is pressed into ASTM standard dog-bone product.With with the press for extracting juice of solvent treatment excessively the Semen Maydis powder of oil do not compare, use through the press for extracting juice of solvent treatment excessively the product that makes of the Semen Maydis powder of oil obvious improved tensile strength is arranged.

Perhaps, Semen Maydis powder of the present invention is with powder: the ratio of weight of oil than 1: 3 is suspended in separately in the aqueous solution of ethanol (95%), and seethes with excitement and refluxed simultaneously and mechanical stirring in 2 hours.Powder filtered and with ethanol (1: 1 resistates: debris ethanol).With resistates drying, dehumidifying and according to top method mold pressing.Under boiling point with Ethanol Treatment 2 hours the tensile strength and the water-intake rate of powder and the powder of at room temperature handling 18 hours be similar.

Embodiment 12

Alcoholic acid is produced

(A) starch hydrolysis

Semen Maydis powder by the solvent extraction of the present invention of method described herein preparation is the source of competent fermentation starch.A kind of method that the soluble sugar that is fit to fermentation is provided is that hydrolysis is contained in the starch molecule in the Semen Maydis powder of solvent extraction.Make about 300g by the Semen Maydis powder of method of the present invention preparation sieve, and in encloses container, they are mixed with water and the 0.5ml α-Dian Fenmei of 99 ℃-100 ℃ of 700ml by 1mm.With alkali pH is transferred to 5.9.Mixture was stirred 45 minutes and add extra α-Dian Fenmei.Cultivate after 45 minutes, transfer to 4.5 with sour pH with mixture.Add half milliliter of (0.5ml) glucoamylase (Optimax 7525) and 0.5g proteolytic enzyme (FungalProtease 5000), and cultivated 22-24 hour down at 62 ℃ together with these two kinds of enzymes.Through this processing, go up with organic acid post (Aminex HPX-87H ion exchange column, 300mm * 7.8mm, BioRad) hydrolysis degree of detection starch at HPLC (Waters 2690 Separations module).

Measure the total nitrogen content of each sample with Leco 2000 CN.Measure free amino acid nitrogen (FAN) with AOAC method (the 15th edition, 1990. the 735th pages).For relatively, prepared broken corn grain and also fermented with similar method.In table 16, listed with comminuting method by the amount of the glucose of starch generation and the amino acid whose amount that in corn sample, obtains.The glucose content that the YDM representative is the highest, the HOC representative is minimum.

High oil corn (HOC) and high oil corn powder (HOCM) have all shown the total nitrogen that is higher than yellow molar shape (YD) and yellow dent corn powder (YDM).HOC and HOCM contain the total free aminoacids more than YD and YDM respectively.In a word, the method that grinds all is the same to all samples basically, because the weight loss that evaporation causes remains between the 4%-5%.

Table 16

Corn sample	Glucose (g/L) *	????FAN(ppm)	Total nitrogen (ppm)
				????YD	????242.40	????261.15	????3437.6
????YDM	????311.28	????195.84	????4009.6
				????HOC	????228.02	????302.95	????4916.0
????HOCM	????240.68	????232.65	????5032.0

^*The explanation value obtains after with amylase and protease treatment

(B) fermentation

In the 125ml flask, add Semen Maydis grit that 45 grams (45g) handle with enzyme and with the Semen Maydis powder (approximately containing 20% carbohydrate) of solvent extraction.Add yeast extract to guarantee that nitrogen without limits with 1g/L.Inoculation 10% on the culture from the yeast culture that spends the night (the Altech ethanol yeast that a kind of typical yeast saccharomyces cerevisiae belongs to) and on the gyrate shaker at 125rpm under 30 ℃, cultivated 42 hours.Measure ethanol production with HPLC.

Studies show that in the past was grown in the yeast that sugared content is close to or higher than on 25% the YD corn and can not obtains maximum ethanol production after 42 hour.Therefore, on the basis of weight, fermentation media is proofreaied and correct, made the content of final sugar fermentation be about 20%.The initial glucose content that contains the culture of YD, YDM, HOC and HOCM is respectively 212.21,236.19,187.85 and 222.77g/L (Fig. 1).Be grown in culture on the HOC and utilized the glucose that exists fully, and other culture has consumed glucose and makes its final concentration less than 1g/L.In having the culture of ground HOC, growth also observed the utmost dispatch of glucose consumption.YD, YDM and HOCM culture have shown similar glucose utilization curve.HOC and HOCM culture have surpassed 80g/L ethanol, but just stop to produce after 19 hours, and this can be because the shortage of essential nutrient causes.Do not have culture can reach 50% theoretical maximum ethanol production, yet the YD culture has reached 45% output, YDM has reached 43%, and HOC has reached 41%, and HOCM has reached 38% (table 17).Maximum ethanol production is approaching relatively, may minimum growth conditions adjusting cause this difference.

Detection to ethanol production shows that the yeast output that is grown on the HOC is the highest, has produced the ethanol (table 17) that surpasses 5g/L/h after 7 hours.After 19 hours, the productive rate of these cultures has descended, yet has just exhausted all glucose before this.Remaining culture has reached maximum yield (greater than 4.5g/L/h) after 19 hours.The alcohol yied value of these four kinds of cultures is very similar.

Table 17

Fermentation media is added with:	Ethanol production (g EtOH/g sugar)				Alcohol yied (g/L/h)
									????7h	????19h	????26h	????42h	????7h	????19h	????26h	????42h
	??YD	????0.10	????0.40	????0.45	????0.41	????3.51	????4.92	????3.97									????2.26
??YDM									????0.12	????0.38	????0.43	????0.40	????3.65	????4.6	????3.81	????2.21
	??HOC	????0.19	????0.41	????0.39	????0.39	????5.14	????4.11	????2.91									????1.76
??HOCM									????0.11	????0.38	????0.36	????0.36	????3.78	????4.6	????3.22	????2.01

For the acidic conditions of guaranteeing to produce during the fermentation can not influence zymic growth and later ethanol generation, regulated the pH (Fig. 2) of substratum.After for some time, the pH of all cultures descends with similar trend, and final pH is between 3.75 and 3.9.There is not tangible pH fluctuation can cause the difference of ethanol production.

Embodiment 13

Contain aquatic feeds by the Semen Maydis powder of high oil corn gained

This embodiment has listed the application of Semen Maydis powder in the aquatic feeds product of pressing oil.

To two kinds of fishes:; Tilapia (tillapia) and catfish are used two kinds of method for breeding.A kind of method for breeding is with the feed that contains the Semen Maydis grit that is made by the yellow corn of dry grinding.Another kind of method for breeding uses the feed that contains from the ECM of high oil corn.Feed is made (table 18) with following composition:

Table 18

Percentage composition

Herring meal 8

Bean powder 50

Corn 34.3

Wheat meal 5

Lin Suanergai 1

Vitamine mixture 1.5

Trace element mixture 0.2

Crude protein (N * 6.25) 32

In the feed grain ration shown in the table 18, the Semen Maydis powder (ECM) that pressed oil can various levels substitute part or all of corn, some or all of wheat meal with and/or the part bean powder, with the required nutritional character that obtains changing according to the fingerling class of being fed.

With containing press for extracting juice one group of tilapia of forage feed of the Semen Maydis powder of oil excessively.With another group tilapia of the forage feed that contains Semen Maydis grit.Equally, with containing press for extracting juice one group of catfish of forage feed of the Semen Maydis powder of oil excessively, with another group catfish of the forage feed that contains Semen Maydis grit.

Test design is handled with 4 pounds at every turn, and per 100 fishes are wanted 16 pounds and 1,600 fish altogether with 1 pound.Every kind of fish all has proximate size and weight.In each kind and each the processing, the typical necessary feeding volume of the speed of growth comes the feed fish in the conventional aquatic products production with satisfying.With fish by one refer to long support to can reflect conventional market weight (as, about 1 pound or half pound) size.

But fish caught out and with a kind of flesh of fish of method manufacturing visual comparison.Can instruct (colorreference guide) to measure the color of tissue with color reference to estimate the effect of oily Semen Maydis powder of pressing to meat quality.Allow trained and experienced sensory test person estimate the factor that the human consumer prefers, as color and outward appearance.

Produce the process of the Semen Maydis powder that pressed oil and from powder, separated the soluble pigment of some solvents.Therefore, compare, in their diet, just accepted less pigment with the fish that the Semen Maydis powder that pressed oil is raised with the fish of raising with the diet that contains corn.When containing pigments such as carotenoid in the feed, they can be deposited in the tissue.Therefore, and compare with the fish of forage feed that contains corn, with contain press for extracting juice excessively the fish of the forage feed of the Semen Maydis powder of oil the more shallow tissue of color is just arranged.With contain press for extracting juice excessively the fish of the forage feed of the Semen Maydis powder of oil growing state be similarly with the fish of forage feed that contains corn, but may need the grain ration components in proportions is adjusted so that the digestibility of starch, amino acid whose utilization ratio and fatty acid content are different.

Embodiment 14

Contain biofuel from the Semen Maydis oil of high oil corn

This embodiment has told about and will be used as the source of improved biodiesel fuel from the oil of high oil corn.

In continuous processing, extraction that will about 62kg/hr (137lbs/hr) in the steel basin reaction member is from high oil corn and by known commercial run purified oil and 18kg/hr (40lbs/hr) methanol mixed.Simultaneously, add 0.08kg/hr (0.1775lbs/hr) sodium hydroxide in the steel basin reaction member, this operation is carried out under 20psig and about 80 ℃.These conditions can 100% transformation efficiency the triglyceride level that adds is transformed into lipid acid and methyl ester.

The two-phase of reaction mixture is left standstill and separate, obtaining methyl ester in mutually, and obtaining the glycerine and the about mixture of residual methyl ester, methyl alcohol and the alkali of 10-15wt.% down in mutually last.About 6.4kg/hr (14lbs.hr) glycerine is neutralized mutually, flash off methyl alcohol, and resistates is delivered in the continuously stirring reaction member of 320psig and 80 ℃ of following operations.It is 2 hours Amberlyst-15 catalyzer that this reaction member also contains about 4wt.% residence time, and adds about 7.9kg/hr (17.5lbs/hr) iso-butylene in reaction member.Speed with about 66kg/hr (145lbs.hr) has made biodiesel fuel, and its dynamic viscous rate and cloud point are greater than the biofuel that does not have glyceryl ester at present.

Incorporate all reference slurries of quoting at this into here with same degree, comprise publication, patent application and patent, for your guidance, this just looks like that each bibliography is all incorporated into for your guidance equally in full by independent also mentioning especially.

The application of (especially in the following claim book) in describing article of the present invention of term " " and " this " and similar words should be interpreted as having comprised odd number and plural number, unless explanation or obvious in the text contradiction are arranged here in addition.Except as otherwise noted, the scope of the value of mentioning here only is as the stenography method of pointing out each independent values in this scope individually, and just as explanation respectively here, each independent values all is contained in this specification sheets.Unless explanation or obvious in the text contradiction are arranged here in addition, and all methods described herein can any suitable order be carried out.Except as otherwise noted, arbitrary and all embodiment that provide here or exemplary language (as, such as) use only be can not be interpreted as in order to set forth the present invention better to limit the scope of the present invention.Any language in this specification sheets all can not be interpreted as having determined any element to the necessary failed call of enforcement of the present invention.

Here described the preferred embodiment of the invention, comprised that the inventor is known for carrying out optimal mode of the present invention.Certainly, by reading the description of front, the variation of those preferred embodiments is conspicuous for the those skilled in the art in this field.The inventor wishes that those skilled in the art suitably use this type of variation, and the inventor wishes that the present invention is used in here and describes beyond the scope especially.Therefore, the present invention includes the equivalent form of value of the main body of mentioning in all modifications and additional claims of being approved by governing law.In addition, unless explanation or obvious in the text contradiction are arranged here in addition, all possible combination of element above-mentioned is also included within the present invention.

Claims (48)

1. Semen Maydis powder remaining behind the complete high oil corn extract oil.

2. Semen Maydis powder as claimed in claim 1 is characterized in that the oil-contg of complete high oil corn is about 6wt.% or higher.

3. Semen Maydis powder as claimed in claim 1 is characterized in that oil-contg is about 7wt.%-30wt.%.

4. Semen Maydis powder as claimed in claim 2 is characterized in that, before the extract oil, complete corn is softened.

5. Semen Maydis powder as claimed in claim 4 is characterized in that, before the extract oil, the remollescent corn is broken.

6. Semen Maydis powder as claimed in claim 5 is characterized in that, before the extract oil, broken corn is by compressing tablet.

7. Semen Maydis powder as claimed in claim 5 is characterized in that, before the extract oil, the remollescent corn is broken.

8. Semen Maydis powder as claimed in claim 7 is characterized in that, before the extract oil, broken corn is wetted.

9. Semen Maydis powder as claimed in claim 8 is characterized in that, before the extract oil, broken corn is by compressing tablet.

One kind processing complete high oil corn method, it comprises:

1) will soften and broken complete high oil corn wetting.

2) with the complete high oil corn compressing tablet of fragmentation; And

3) extraction is pressed into the corn of thin slice to make Semen Maydis powder and Semen Maydis oil.

11. method as claimed in claim 10, it further comprises complete high oil corn remollescent step.

12. method as claimed in claim 11 is characterized in that, wetting step is that complete high oil corn is being carried out before the softening step.

13. method as claimed in claim 10, it further comprises the step with complete high oil corn fragmentation.

14. method as claimed in claim 11, it further comprises complete high oil corn remollescent step.

15. method as claimed in claim 13 is characterized in that, broken step is to carry out before with the wetting step of complete high oil corn.

16. method as claimed in claim 14 is characterized in that, broken step is after softening step, and before complete high oil corn is carried out wetting step, carry out.

17. method as claimed in claim 10 is characterized in that, the oil-contg of complete corn is about 6wt.% or higher.

18. method as claimed in claim 10 is characterized in that, the oil-contg of complete corn is about 7wt.%-30wt.%.

19. method as claimed in claim 10 is characterized in that, extraction step is to finish by extracting Semen Maydis oil with continuous solvent-extraction process from the corn grain that is pressed into thin slice.

20. method as claimed in claim 19 is characterized in that, the corn that is pressed into thin slice contacts one period that is enough to extract the oil of aequum with solvent.

21. method as claimed in claim 19 is characterized in that, the corn and the solvent that are pressed into thin slice kept in touch 10 minutes at least.

22. method as claimed in claim 10 is characterized in that, the thickness of thin slice is about 0.1mm-1.0mm.

23. method as claimed in claim 19 is characterized in that, contains hexane in the solvent.

24. produce the alcoholic acid method for one kind, it comprises:

1) with Semen Maydis powder as claimed in claim 1 and water and α-Dian Fenmei mixing;

2) cultivate mixture and in mixture, add at least a additive; And

3) mixture is mixed to produce ethanol with microorganism that can fermenting carbon source.

25. method as claimed in claim 24 is characterized in that, additive is selected from glucoamylase and proteolytic enzyme.

26. biofuel that contains the Semen Maydis oil that makes by extract oil from complete high oil corn.

27. the method that produces in the process that is recovered in the processing high oil corn than light grains, it be included in feed air-flow on the high oil corn particle so that lighter particle along with air-flow is taken out of, thereby particle that will be lighter and heavier particle separation.

28. method as claimed in claim 27 is characterized in that, gas is selected from air, nitrogen and argon gas.

29. the method than light grains that produces in the process that is recovered in the processing high oil corn, it is included in and feeds liquid spray on the high oil corn particle taking lighter particle out of, thus particle that will be lighter and heavier particle separation.

30. method as claimed in claim 29 is characterized in that, liquid spray is a water.

31. method as claimed in claim 30 is characterized in that, liquid spray further contains at least a composition that is selected from VITAMIN, mineral substance, enzyme and their combination.

32. method as claimed in claim 31 is characterized in that, liquid spray further contains caustic liquor.

33. biodegradable product that origin is made from the Semen Maydis powder through solvent extraction of high oil corn.

34. biodegradable product as claimed in claim 33 is characterized in that the Semen Maydis powder of solvent extraction is handled through organic solvent.

35. biodegradable product as claimed in claim 33 is characterized in that the Semen Maydis powder of extraction is further handled through linking agent.

36. biodegradable product as claimed in claim 35 is characterized in that, linking agent is selected from aldehyde, acid anhydrides, epoxide or their combination.

37. feed that contains the Semen Maydis powder that makes by the oil of solvent extraction from complete high oil corn.

38. feed as claimed in claim 37 is characterized in that, feed is animal-feed or aquaculture feed.

39. feed as claimed in claim 38 is characterized in that, animal-feed is the chicken feed.

40. feed as claimed in claim 39 is characterized in that, feed is an aquaculture feed.

41. extract Semen Maydis oil from the laminar corn gained of complete oily corn.

42. Semen Maydis oil as claimed in claim 41 is characterized in that, the oil-contg of complete high oil corn is about 6wt% or higher.

43. Semen Maydis oil as claimed in claim 42 is characterized in that oil-contg is about 7wt.%-30wt.%.

44. Semen Maydis oil as claimed in claim 42 is characterized in that, extracts to be pressed into before the corn of thin slice, complete corn is softened.

45. Semen Maydis powder as claimed in claim 44 is characterized in that, extracts to be pressed into before the corn of thin slice, the remollescent corn is broken.

46. Semen Maydis powder as claimed in claim 45 is characterized in that, extracts to be pressed into before the corn of thin slice, broken corn is by compressing tablet.

47. Semen Maydis powder as claimed in claim 45 is characterized in that, extracts to be pressed into before the corn of thin slice, the remollescent corn is broken.

48. Semen Maydis powder as claimed in claim 47 is characterized in that, extracts to be pressed into before the corn of thin slice, broken corn is wetted.

Images