NO319618B1 - Fish feed containing tocotrienol to improve the stability and pigment distribution in the fish fillet and processed fish products. - Google Patents
Fish feed containing tocotrienol to improve the stability and pigment distribution in the fish fillet and processed fish products. Download PDFInfo
- Publication number
- NO319618B1 NO319618B1 NO20023262A NO20023262A NO319618B1 NO 319618 B1 NO319618 B1 NO 319618B1 NO 20023262 A NO20023262 A NO 20023262A NO 20023262 A NO20023262 A NO 20023262A NO 319618 B1 NO319618 B1 NO 319618B1
- Authority
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- Norway
- Prior art keywords
- fish
- tocotrienol
- fillet
- stability
- tocopherol
- Prior art date
Links
- 241000251468 Actinopterygii Species 0.000 title claims description 38
- 239000011731 tocotrienol Substances 0.000 title claims description 32
- 229930003802 tocotrienol Natural products 0.000 title claims description 28
- 235000019148 tocotrienols Nutrition 0.000 title claims description 28
- GJJVAFUKOBZPCB-UHFFFAOYSA-N 2-methyl-2-(4,8,12-trimethyltrideca-3,7,11-trienyl)-3,4-dihydrochromen-6-ol Chemical compound OC1=CC=C2OC(CCC=C(C)CCC=C(C)CCC=C(C)C)(C)CCC2=C1 GJJVAFUKOBZPCB-UHFFFAOYSA-N 0.000 title claims description 22
- 238000009826 distribution Methods 0.000 title claims description 5
- 239000000049 pigment Substances 0.000 title claims description 4
- 235000013332 fish product Nutrition 0.000 title claims description 3
- 235000019688 fish Nutrition 0.000 claims description 38
- 241000972773 Aulopiformes Species 0.000 claims description 13
- 235000019515 salmon Nutrition 0.000 claims description 13
- 235000021323 fish oil Nutrition 0.000 claims description 9
- 239000006227 byproduct Substances 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 6
- 241000277331 Salmonidae Species 0.000 claims description 4
- 239000001054 red pigment Substances 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 claims description 3
- 241001290266 Sciaenops ocellatus Species 0.000 claims description 2
- 235000015090 marinades Nutrition 0.000 claims description 2
- 239000000853 adhesive Substances 0.000 claims 1
- 230000001070 adhesive effect Effects 0.000 claims 1
- 150000001720 carbohydrates Chemical class 0.000 claims 1
- 235000014633 carbohydrates Nutrition 0.000 claims 1
- 239000000945 filler Substances 0.000 claims 1
- 229910052500 inorganic mineral Inorganic materials 0.000 claims 1
- 150000002632 lipids Chemical class 0.000 claims 1
- 239000011707 mineral Substances 0.000 claims 1
- 239000003755 preservative agent Substances 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 230000000391 smoking effect Effects 0.000 claims 1
- 229930003231 vitamin Natural products 0.000 claims 1
- 235000013343 vitamin Nutrition 0.000 claims 1
- 239000011782 vitamin Substances 0.000 claims 1
- 229940088594 vitamin Drugs 0.000 claims 1
- GVJHHUAWPYXKBD-UHFFFAOYSA-N d-alpha-tocopherol Natural products OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 42
- GVJHHUAWPYXKBD-IEOSBIPESA-N α-tocopherol Chemical compound OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-IEOSBIPESA-N 0.000 description 31
- 239000011732 tocopherol Substances 0.000 description 26
- 229960001295 tocopherol Drugs 0.000 description 23
- 229930003799 tocopherol Natural products 0.000 description 20
- 235000010384 tocopherol Nutrition 0.000 description 18
- 239000003963 antioxidant agent Substances 0.000 description 15
- 235000006708 antioxidants Nutrition 0.000 description 15
- 230000000694 effects Effects 0.000 description 12
- 230000003078 antioxidant effect Effects 0.000 description 11
- 239000000203 mixture Substances 0.000 description 11
- 229930003427 Vitamin E Natural products 0.000 description 10
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 10
- 239000003921 oil Substances 0.000 description 10
- 235000019198 oils Nutrition 0.000 description 10
- 229940046009 vitamin E Drugs 0.000 description 10
- 235000019165 vitamin E Nutrition 0.000 description 10
- 239000011709 vitamin E Substances 0.000 description 10
- 239000000523 sample Substances 0.000 description 8
- GJJVAFUKOBZPCB-ZGRPYONQSA-N (r)-3,4-dihydro-2-methyl-2-(4,8,12-trimethyl-3,7,11-tridecatrienyl)-2h-1-benzopyran-6-ol Chemical class OC1=CC=C2OC(CC/C=C(C)/CC/C=C(C)/CCC=C(C)C)(C)CCC2=C1 GJJVAFUKOBZPCB-ZGRPYONQSA-N 0.000 description 6
- 229960000984 tocofersolan Drugs 0.000 description 6
- 229940068778 tocotrienols Drugs 0.000 description 6
- 239000002076 α-tocopherol Substances 0.000 description 6
- 235000004835 α-tocopherol Nutrition 0.000 description 6
- 150000002148 esters Chemical class 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 230000006698 induction Effects 0.000 description 4
- 235000013372 meat Nutrition 0.000 description 4
- 230000015556 catabolic process Effects 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 238000006731 degradation reaction Methods 0.000 description 3
- 238000009472 formulation Methods 0.000 description 3
- 230000003647 oxidation Effects 0.000 description 3
- 238000007254 oxidation reaction Methods 0.000 description 3
- 230000001590 oxidative effect Effects 0.000 description 3
- 235000015277 pork Nutrition 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 229940119224 salmon oil Drugs 0.000 description 3
- 239000013589 supplement Substances 0.000 description 3
- YYGNTYWPHWGJRM-UHFFFAOYSA-N (6E,10E,14E,18E)-2,6,10,15,19,23-hexamethyltetracosa-2,6,10,14,18,22-hexaene Chemical compound CC(C)=CCCC(C)=CCCC(C)=CCCC=C(C)CCC=C(C)CCC=C(C)C YYGNTYWPHWGJRM-UHFFFAOYSA-N 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- BHEOSNUKNHRBNM-UHFFFAOYSA-N Tetramethylsqualene Natural products CC(=C)C(C)CCC(=C)C(C)CCC(C)=CCCC=C(C)CCC(C)C(=C)CCC(C)C(C)=C BHEOSNUKNHRBNM-UHFFFAOYSA-N 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 229940087168 alpha tocopherol Drugs 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- PRAKJMSDJKAYCZ-UHFFFAOYSA-N dodecahydrosqualene Natural products CC(C)CCCC(C)CCCC(C)CCCCC(C)CCCC(C)CCCC(C)C PRAKJMSDJKAYCZ-UHFFFAOYSA-N 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 230000003244 pro-oxidative effect Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 238000004064 recycling Methods 0.000 description 2
- 238000003307 slaughter Methods 0.000 description 2
- 229940031439 squalene Drugs 0.000 description 2
- TUHBEKDERLKLEC-UHFFFAOYSA-N squalene Natural products CC(=CCCC(=CCCC(=CCCC=C(/C)CCC=C(/C)CC=C(C)C)C)C)C TUHBEKDERLKLEC-UHFFFAOYSA-N 0.000 description 2
- 230000006641 stabilisation Effects 0.000 description 2
- 238000011105 stabilization Methods 0.000 description 2
- 230000000087 stabilizing effect Effects 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 230000002195 synergetic effect Effects 0.000 description 2
- 235000019149 tocopherols Nutrition 0.000 description 2
- 230000004584 weight gain Effects 0.000 description 2
- 235000019786 weight gain Nutrition 0.000 description 2
- QUEDXNHFTDJVIY-UHFFFAOYSA-N γ-tocopherol Chemical class OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1 QUEDXNHFTDJVIY-UHFFFAOYSA-N 0.000 description 2
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 description 1
- OTXNTMVVOOBZCV-UHFFFAOYSA-N 2R-gamma-tocotrienol Natural products OC1=C(C)C(C)=C2OC(CCC=C(C)CCC=C(C)CCC=C(C)C)(C)CCC2=C1 OTXNTMVVOOBZCV-UHFFFAOYSA-N 0.000 description 1
- ZAKOWWREFLAJOT-CEFNRUSXSA-N D-alpha-tocopherylacetate Chemical compound CC(=O)OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C ZAKOWWREFLAJOT-CEFNRUSXSA-N 0.000 description 1
- 150000000996 L-ascorbic acids Chemical class 0.000 description 1
- QAQJMLQRFWZOBN-LAUBAEHRSA-N L-ascorbyl-6-palmitate Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](O)[C@H]1OC(=O)C(O)=C1O QAQJMLQRFWZOBN-LAUBAEHRSA-N 0.000 description 1
- 239000011786 L-ascorbyl-6-palmitate Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- RZFHLOLGZPDCHJ-DLQZEEBKSA-N alpha-Tocotrienol Natural products Oc1c(C)c(C)c2O[C@@](CC/C=C(/CC/C=C(\CC/C=C(\C)/C)/C)\C)(C)CCc2c1C RZFHLOLGZPDCHJ-DLQZEEBKSA-N 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- 235000010385 ascorbyl palmitate Nutrition 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 235000015165 citric acid Nutrition 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000000787 lecithin Substances 0.000 description 1
- 235000010445 lecithin Nutrition 0.000 description 1
- 229940067606 lecithin Drugs 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000003278 mimic effect Effects 0.000 description 1
- 230000036284 oxygen consumption Effects 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 230000019612 pigmentation Effects 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 239000012747 synergistic agent Substances 0.000 description 1
- 229940042585 tocopherol acetate Drugs 0.000 description 1
- 150000003712 vitamin E derivatives Chemical class 0.000 description 1
- RZFHLOLGZPDCHJ-XZXLULOTSA-N α-Tocotrienol Chemical compound OC1=C(C)C(C)=C2O[C@@](CC/C=C(C)/CC/C=C(C)/CCC=C(C)C)(C)CCC2=C1C RZFHLOLGZPDCHJ-XZXLULOTSA-N 0.000 description 1
- QUEDXNHFTDJVIY-DQCZWYHMSA-N γ-tocopherol Chemical compound OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1 QUEDXNHFTDJVIY-DQCZWYHMSA-N 0.000 description 1
- OTXNTMVVOOBZCV-WAZJVIJMSA-N γ-tocotrienol Chemical compound OC1=C(C)C(C)=C2O[C@@](CC/C=C(C)/CC/C=C(C)/CCC=C(C)C)(C)CCC2=C1 OTXNTMVVOOBZCV-WAZJVIJMSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B4/00—General methods for preserving meat, sausages, fish or fish products
- A23B4/14—Preserving with chemicals not covered by groups A23B4/02 or A23B4/12
- A23B4/18—Preserving with chemicals not covered by groups A23B4/02 or A23B4/12 in the form of liquids or solids
- A23B4/20—Organic compounds; Microorganisms; Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B4/00—General methods for preserving meat, sausages, fish or fish products
- A23B4/044—Smoking; Smoking devices
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
- A23L17/70—Comminuted, e.g. emulsified, fish products; Processed products therefrom such as pastes, reformed or compressed products
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Husbandry (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- General Chemical & Material Sciences (AREA)
- Insects & Arthropods (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Birds (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Description
Denne oppfinnelsen vedrører et nytt fiskefor, anvendelse av en spesiell antioksidant i fiskefor, så vel som en fremgangsmåte for å forbedre stabiliteten av kjøttet i alle oppdretts-fiskearter og en fremgangsmåte for å stabilisere pigmenteringen av røde fiskear- This invention relates to a new fish feed, the use of a special antioxidant in fish feed, as well as a method for improving the stability of the meat in all farmed fish species and a method for stabilizing the pigmentation of red fish livers.
ter. Oppfinnelsen vedrører også stabilisering av biprodukter fra oppdrettsfisk inkludert fiskeolje. ter. The invention also relates to the stabilization of by-products from farmed fish, including fish oil.
På grunn av det relativt høye fettinnholdet i noen fiskearter, foregår en degradering av kvaliteten av fisken forårsaket av harskning etter slakting. Hastigheten av denne degra-deringen bestemmer levetiden på fiskefileten. Harskning er en viktig faktor ved kvali-tetsvurdering både for fersk og bearbeidet fisk. For distributørene av fisk og fiskefilet er det av stor interesse å utvide levetiden til produktet for alle fisketyper. Due to the relatively high fat content of some fish species, a degradation of the quality of the fish takes place caused by rancidity after slaughter. The speed of this degradation determines the lifespan of the fish fillet. Rancidity is an important factor when assessing the quality of both fresh and processed fish. For the distributors of fish and fish fillets, it is of great interest to extend the lifespan of the product for all types of fish.
For røde oppdrettsfisk slik som laks og ørret kan videre det røde pigmentet i fiskefileten gradvis degraderes og fordeles ujevnt etter bearbeiding eller lagring. Dette er naturligvis ikke tiltalende for kundene. For røkt fiskefilet er dette et særskilt problem, da en gulak- For red farmed fish such as salmon and trout, the red pigment in the fish fillet can also gradually degrade and become unevenly distributed after processing or storage. This is naturally not appealing to customers. For smoked fish fillets, this is a particular problem, as a yellowish
tig kant ofte kommer til syne på fiskefileten når den er røkt. tig edge often appears on the fish fillet when it is smoked.
Det er et hovedformål ved den foreliggende oppfinnelsen å tilveiebringe oppdrettsfisk med forbedret stabilitet av kjøtt og pigment. Et annet formål er å forbedre stabiliteten av bearbeidet fisk, biprodukter og fiskeolje som oppnås fra oppdrettsfisk. It is a main purpose of the present invention to provide farmed fish with improved stability of meat and pigment. Another purpose is to improve the stability of processed fish, by-products and fish oil obtained from farmed fish.
Dette og andre formål oppnås i henhold til de vedheftede kravene. This and other purposes are achieved in accordance with the attached requirements.
Den generelle strukturen av tokoferol er som følger: The general structure of tocopherol is as follows:
der; there;
Slik det her er benyttet omfatter betegnelsen "tokoferol" en hvilken som helst av a-, p-, Y- og 8-tokoferolisomerene alene, alle blandinger av de nevnte isomerene, så vel som derivater av disse slik som estere. As used herein, the term "tocopherol" includes any of the α-, β-, Y- and 8-tocopherol isomers alone, all mixtures of said isomers, as well as derivatives thereof such as esters.
For å unngå degradering av tokoferol ved fremstilling og lagring av fiskefor, tilsettes tokoferol normalt på esterform, for eksempel tokoferolacetat. To avoid degradation of tocopherol during the production and storage of fish feed, tocopherol is normally added in ester form, for example tocopherol acetate.
Den generelle strukturen til tokotrienol er som følger: The general structure of tocotrienol is as follows:
der; there;
Slik den her er benyttet omfatter betegnelsen "tokotrienol" en hvilken som helst av a-, p\ y- og 5-tokotrienolisomeren alene, alle blandinger av disse isomerene, så vel som derivater av disse slik som estere. As used herein, the term "tocotrienol" includes any of the α-, β-, y- and 5-tocotrienol isomers alone, all mixtures of these isomers, as well as derivatives thereof such as esters.
En fagmann på området vil forstå at på samme måte som tokoferol over, bør tokotrienol tilsettes på formen av et derivat som ikke lett degraderes på grunn av oksidasjon. Det vil si at derivater slik som estere er en foretrukket form av tokotrienolene som benyttes ved den foreliggende oppfinnelsen. One skilled in the art will understand that, in the same way as tocopherol above, tocotrienol should be added in the form of a derivative that is not easily degraded due to oxidation. That is to say that derivatives such as esters are a preferred form of the tocotrienols used in the present invention.
Oppfinnelsen er forklart i videre detalj nedenfor med referanse til figur 1 og 2. The invention is explained in further detail below with reference to figures 1 and 2.
Figur 1 viser effekten av forsterkning av fersk laksefilet med vitamin E på form av tokoferoler sammenlignet med filet som er forsterket med vitamin E på form av tokotrienoler. Figur 2 viser effekten av tilsetning av a-tokoferol med hensyn på stabilitet/holdbarhets-tid på lakseolje som fremstilles ved mild bearbeiding av ferske laksefilet-biprodukter. Figur 3 viser effekten av tilsetning av ytterligere a-tokoferol eller -tokotrienol med hensyn til stabilitet/holdbarhetstid for lakseolje fremstilt ved mild bearbeiding av ferske laksefilet-biprodukter. Figure 1 shows the effect of fortification of fresh salmon fillets with vitamin E in the form of tocopherols compared to fillets fortified with vitamin E in the form of tocotrienols. Figure 2 shows the effect of adding α-tocopherol with regard to stability/shelf life of salmon oil produced by mild processing of fresh salmon fillet by-products. Figure 3 shows the effect of adding additional α-tocopherol or -tocotrienol with regard to stability/shelf life for salmon oil produced by mild processing of fresh salmon fillet by-products.
Sener, V.A. og Ivanov, S.A. (1973) "Natural antioxidants I: The antioxidant effect on tocotrienols", Fette Seifen Anstri 75, s. 606-608 rapporterer at tokotrienoler var mer aktive enn det tilsvarende tokoferol i flesk. Det fremgår her at antioksidantene er blandet i smeltet svinefett (50 °C), og det er funnet at tokotrienoler gir ca. 5 % høyere aktivitet enn de tilsvarende tokoferoler. Denne svake økningen av aktivitet mener forfatterne skyldes umettethet i sidekjeden, og de blander, i et separat forsøk, tokoferol og skvalen for å etterligne dette. Denne blandingen gir tilsvarende effekt som tokotrienol, dvs. en svak økning i effekt. Til dette er å bemerke at 5 % økt aktivitet ville være for lite til å kunne tjene de formål som ønskes oppnådd ved foreliggende oppfinnelse. Videre inneholder fiskefett, i motsetning til svinefett, store konsentrasjoner av umettede forbindelser. En fagmann på området vil derfor forstå at fiskeolje ville inneholdt tilstrekkelige mengder skvalen, og/eller andre umettede forbindelser med lignende struktur, til at eventuell synergi med tokoferol ville bli oppfylt. En fagmann på området vil derfor ikke se noe i den aktuelle artikkel som kunne lede til at tokotrienol ved bruk i for som i foreliggende oppfinnelse, ville gi en god beskyttende effekt av fiskefilet, i motsetning til tokoferol som ikke har gitt noen påvisbar effekt. Sener, V.A. and Ivanov, S.A. (1973) "Natural antioxidants I: The antioxidant effect on tocotrienols", Fette Seifen Anstri 75, pp. 606-608 reports that tocotrienols were more active than the corresponding tocopherol in pork. It appears here that the antioxidants are mixed in melted pork fat (50 °C), and it has been found that tocotrienols provide approx. 5% higher activity than the corresponding tocopherols. This slight increase in activity the authors believe is due to unsaturation in the side chain, and they mix, in a separate experiment, tocopherol and squalene to mimic this. This mixture gives a similar effect to tocotrienol, i.e. a slight increase in effect. In addition to this, it should be noted that a 5% increased activity would be too little to be able to serve the purposes desired to be achieved by the present invention. Furthermore, fish fat, unlike pork fat, contains large concentrations of unsaturated compounds. A person skilled in the art will therefore understand that fish oil would contain sufficient amounts of squalene, and/or other unsaturated compounds with a similar structure, that any synergy with tocopherol would be fulfilled. A person skilled in the field will therefore not see anything in the article in question which could lead to the fact that tocotrienol, when used as in the present invention, would give a good protective effect of the fish fillet, in contrast to tocopherol which has not given any detectable effect.
NO 301223 vedrører tokotrienol i humane tilskudd så som kosttilskudd og farmasøytiske preparater. Tilskuddene angis å ha biologiske effekter når det gjelder å forbedre helsetilstanden og å forhindre sykdommer hos mennesker. Det er her snakk om effekt in vivo. I foreliggende søknad er det snakk om effekt post mortem. Det er ikke nevnt noe i NO 301223 om at tokotrienol har positiv effekt på forlengelse av holdbarhet i næringdmiddelprodukter. NO 301223 relates to tocotrienol in human supplements such as dietary supplements and pharmaceutical preparations. The supplements are stated to have biological effects when it comes to improving the state of health and preventing diseases in humans. Here we are talking about effect in vivo. In the present application, we are talking about post-mortem effects. There is no mention in NO 301223 that tocotrienol has a positive effect on extending the shelf life of food products.
EP 0577305 vedrører en fremgangsmåte for å stabilisere flerumettede materialer ved tilsetning av askorbinsyreester og fosforylerte mono-/diglycerider. Tokotrienol eller tokoferol kan eventuelt også tilsettes. I EP 0577305 er tokotrienol altså ikke det virksomme prinsippet, men kun en eventualitet. Videre likestilles her tokotrienol og tokoferol. Kun bruk av tokoferol er vist i eksempelmaterialet. EP 0577305 relates to a method for stabilizing polyunsaturated materials by adding ascorbic acid esters and phosphorylated mono-/diglycerides. Tocotrienol or tocopherol can optionally also be added. In EP 0577305, tocotrienol is thus not the active principle, but only an eventuality. Furthermore, tocotrienol and tocopherol are equated here. Only the use of tocopherol is shown in the sample material.
WO 00/57876 vedrører en antioksidantformulering som innbefatter tokotrienol og et resirkulerende middel slik at det oppnås en "evigvarende" tokotrienolkilde. Det resirkulerende middelet som er benyttet i eksemplene er askorbinsyre. Denne antioksidantformuleringen kan administreres til pasienter ved behandling av visse sykdommer. WO 00/57876 nevner intet om at antioksidantformuleringen er relevant for fiskefor, eller for forbedring av kvaliteten/holdbarheten av laks etter slakting. WO 00/57876 relates to an antioxidant formulation comprising tocotrienol and a recycling agent so that a "perpetual" source of tocotrienol is obtained. The recycling agent used in the examples is ascorbic acid. This antioxidant formulation can be administered to patients in the treatment of certain diseases. WO 00/57876 mentions nothing about the antioxidant formulation being relevant for fish feed, or for improving the quality/durability of salmon after slaughter.
Rodriguez-Amaya, D.B., "Stability of Carotenoids during the storage of foods", Chemical, Biological, Physical and Nutritional Aspects, 1993, Elsevier Science Publishers B.V. omtaler at karotenoider fra rød pepper kan stabiliseres av tokoferol og askorbinsyre. Det nevnes intet om tokotrienol i denne artikkelen. Både tokoferol og askorbinsyre tilsettes til alt kommersielt fiskefor. Ved foreliggende oppfinnelse er det vist at ytterligere tilsetning av tokoferol utover det som finnes i kommersielt for, gir negativ effekt. Rodriguez-Amaya, D.B., "Stability of Carotenoids during the storage of foods", Chemical, Biological, Physical and Nutritional Aspects, 1993, Elsevier Science Publishers B.V. mentions that carotenoids from red pepper can be stabilized by tocopherol and ascorbic acid. There is no mention of tocotrienol in this article. Both tocopherol and ascorbic acid are added to all commercial fish feed. In the present invention, it has been shown that further addition of tocopherol beyond what is found in commercial flour has a negative effect.
Det er kjent at tilsetning av antioksidanter til foret kan øke antioksidantnivået og som en konsekvens av dette, stabiliteten av fiskekjøttet. (Frigg, M, Prabucki, A.L., Ruhdel, E.U.; Aquaculture 84 (1990) 145, Boggio, S.M., Hardy, R.W., Babbitt, J.K., Brannon, E.L.; Aquaculture 51 (1985) 13, Waagbø, R., Sandnes, K., Torrissen, O.J., Sandvin, A., Lie, 0., Food Chemistry 46 (1993) 361). Kommersielt fiskefor er tilsatt vitamin E på form av a-tokoferylacetat, i den hensikt å være virkningsfull i fisken. It is known that the addition of antioxidants to the feed can increase the antioxidant level and, as a consequence, the stability of the fish meat. (Frigg, M, Prabucki, A.L., Ruhdel, E.U.; Aquaculture 84 (1990) 145, Boggio, S.M., Hardy, R.W., Babbitt, J.K., Brannon, E.L.; Aquaculture 51 (1985) 13, Waagbø, R., Sandnes, K., Torrissen, O.J., Sandvin, A., Lie, 0., Food Chemistry 46 (1993) 361). Commercial fish feed has added vitamin E in the form of a-tocopheryl acetate, with the intention of being effective in the fish.
Det er også kjent at de optimale nivåene av vitamin E, for å ha antioksidativ effekt, er forskjellig mellom forskjellige matrikser. Dette er bekreftet at våre eksperimenter. It is also known that the optimal levels of vitamin E, to have an antioxidative effect, differ between different matrices. This is confirmed by our experiments.
Vi har vist at ytterligere tilsetning av vitamin E, enten som den isolerte D-a-isomeren eller som en blanding av a-, p-, y- og 5-isomerene, til en fiskeolje som er fremstilt fra ferske råmaterialer under milde betingelser, eller til en fersk filet, ikke resulterer i noen økt stabilitet. We have shown that further addition of vitamin E, either as the isolated D-a isomer or as a mixture of the a-, p-, y- and 5-isomers, to a fish oil produced from fresh raw materials under mild conditions, or to a fresh fillet, does not result in any increased stability.
Når fiskeolje fremstilles fra ferske råmaterialer og under milde betingelser, har vi funnet at den allerede inneholder nært opptil optimale nivåer av tokoferol (D-a-tokoferol) når den oksidative stabiliteten av oljen målt som dens induksjonstid betraktes. Tilsetning av D-a-tokoferol alene ga svake pro-oksidative effekter (se fig. 2). Imidlertid var ytterligere stabilisering mulig ved tilsetningen av en kombinasjon av ascorbylpalmitat, sitron-syre og lecitin (se fig. 3). Å inkludere tokoferol i antioksidant-tilsetningen ga ikke ytterligere positive resultater. When fish oil is produced from fresh raw materials and under mild conditions, we have found that it already contains close to optimal levels of tocopherol (D-a-tocopherol) when the oxidative stability of the oil as measured by its induction time is considered. Addition of D-α-tocopherol alone produced weak pro-oxidative effects (see Fig. 2). However, further stabilization was possible by the addition of a combination of ascorbyl palmitate, citric acid and lecithin (see Fig. 3). Including tocopherol in the antioxidant supplement did not provide additional positive results.
Imidlertid har vi overraskende funnet at forsterkning av oljen eller fileten med vitamin E-analoger på formen av tokotrienoler, resulterer i økt stabilitet. Det er åpenbart at en foretrukket måte for å tilføre denne antioksidanten til fiskekjøttet og til fiskeoljen, ville være via foret. Alternativt kan antioksidanten tilsettes til en marinade, hvori fileten ned-senkes for å forbedre stabiliteten av fileten og pigmentfordelingen i fileten. Ved fremstilling av bearbeidede fiskeprodukter kan antioksidanten tilsettes til malt filet. Den kan også tilsettes til oljen som oppnås fra biprodukter av fisk. However, we have surprisingly found that fortification of the oil or fillet with vitamin E analogues in the form of tocotrienols results in increased stability. It is obvious that a preferred way of adding this antioxidant to the fish meat and to the fish oil would be via the feed. Alternatively, the antioxidant can be added to a marinade, in which the fillet is submerged to improve the stability of the fillet and the pigment distribution in the fillet. In the production of processed fish products, the antioxidant can be added to ground fillet. It can also be added to the oil obtained from fish by-products.
Eksempel 1 Example 1
19,6 ppm vitamin E, som besto av 14% d-a-tokoferol, 1% d-P-tokoferol, 62% d-y-tokoferol og 23% d-6-tokoferol, ble malt sammen med en filetprøve fra oppdrettslaks. 19.6 ppm vitamin E, which consisted of 14% d-α-tocopherol, 1% d-P-tocopherol, 62% d-γ-tocopherol and 23% d-6-tocopherol, was ground together with a fillet sample from farmed salmon.
16,3 ppm vitamin E, som besto av 23,5% d-a-tokoferol, 25,5% d-a-tokotrienol, 41,2% d-y-tokotrienol og 9,8% d-8-tokotrienol, ble malt sammen med en annen filetprøve fra oppdrettslaks (vist som tokotrienol i figur 1). 16.3 ppm vitamin E, which consisted of 23.5% d-α-tocopherol, 25.5% d-α-tocotrienol, 41.2% d-γ-tocotrienol and 9.8% d-8-tocotrienol, was ground together with another fillet sample from farmed salmon (shown as tocotrienol in Figure 1).
Som en kontroll ble malt, fersk oppdretts-laksefilet uten ytterligere tilsetning av antioksidanter benyttet. As a control, ground, fresh farmed salmon fillet without further addition of antioxidants was used.
Den relative stabiliteten av disse prøvene i forhold til oksidasjon ble målt i en akselerert test, ved å benytte Oxipres-apparatet. Prøvene ble veiet inn i separate celler, der de ble utsatt for 60°C og 4 bar lufttrykk. Oksygenforbruket forårsaket av reaksjonen av prøven med oksygen i luften, ble registrert som et trykkfall. The relative stability of these samples in relation to oxidation was measured in an accelerated test, using the Oxipres apparatus. The samples were weighed into separate cells, where they were exposed to 60°C and 4 bar air pressure. The oxygen consumption caused by the reaction of the sample with oxygen in the air was recorded as a pressure drop.
Disse eksperimentene viste overraskende at tilsetning av tokotrienol resulterte i en sig-nifikant additiv beskyttende effekt, vist ved at trykkfallet i disse cellene var langsom-mere enn i cellene med kontroll eller tokoferol- (CoviOx) forsterkede prøver. Tilsetningen av tokoferol stabiliserte ikke prøvene relativt til kontrollen. These experiments surprisingly showed that the addition of tocotrienol resulted in a significant additive protective effect, shown by the fact that the pressure drop in these cells was slower than in the cells with control or tocopherol (CoviOx) reinforced samples. The addition of tocopherol did not stabilize the samples relative to the control.
Eksempel 2 Example 2
Fiskeolje fremstilt fra råmaterialer av fersk laks inneholder nært opptil optimale nivåer av tokoferol. Som illustrert i figur 2 økte ikke ytterligere tilsetning av d-a-tokoferol til denne oljen stabiliteten, men ga isteden en liten pro-oksidativ effekt. Oljestabiliteten ble målt som induksjonstiden (i dager) ved vektøknings-eksperiment. Olje A er en lakseolje med 200 ppm d-a-tokoferol. Olje E er kontrollen uten tilsetninger. Fish oil produced from fresh salmon raw materials contains close to optimal levels of tocopherol. As illustrated in Figure 2, further addition of d-α-tocopherol to this oil did not increase stability, but instead produced a small pro-oxidative effect. The oil stability was measured as the induction time (in days) by weight gain experiment. Oil A is a salmon oil with 200 ppm d-a-tocopherol. Oil E is the control without additives.
Til en prøve av fersk fiskeolje fremstilt ved mild bearbeiding av ferske laksefilet-biprodukter (innvoller, hoder, ben), tilsatte vi 200 ppm vitamin E bestående av 89 - 99% d-a-tokoferol og 1 - 11% (p + y + 6)-tokoferoler (ifølge produktspesifikasjon), og en blanding av andre -/synergistiske midler. To a sample of fresh fish oil produced by mild processing of fresh salmon fillet by-products (guts, heads, bones), we added 200 ppm vitamin E consisting of 89 - 99% d-a-tocopherol and 1 - 11% (p + y + 6) -tocopherols (according to product specification), and a mixture of other -/synergistic agents.
Til en annen prøve av oljen tilsatte vi 200 ppm vitamin E bestående av 14% d-a-tokoferol, 1% d-p-tokoferol, 62% d-y-tokoferol og 23% d-8-tokoferol, og en blanding av andre -/synergistiske antioksidanter lignende med hva som ble gitt til den første prøven. To another sample of the oil we added 200 ppm vitamin E consisting of 14% d-a-tocopherol, 1% d-p-tocopherol, 62% d-y-tocopherol and 23% d-8-tocopherol, and a mixture of other -/synergistic antioxidants like with what was given to the first sample.
Til en tredje prøve av oljen tilsatte vi 200 ppm vitamin E bestående av 23,5% d-a-tokoferol, 25,5% d-a-tokotrienol, 41,2% d-y-tokotrienol og 9,8% d-5-tokotrienol, og en blanding av andre -/synergistiske antioksidanter lignende med hva som blitt gitt til de andre prøvene. To a third sample of the oil we added 200 ppm vitamin E consisting of 23.5% d-a-tocopherol, 25.5% d-a-tocotrienol, 41.2% d-y-tocotrienol and 9.8% d-5-tocotrienol, and a mixture of other -/synergistic antioxidants similar to what has been given to the other samples.
Den oksidative stabiliteten av de tre oljene ble testet ved å utsette 3,0 g prøver i 6,0 cm glass-petriskåler for en temperatur på 35°C ved eksponering av luft. Oksygenopptaket forårsaket av oksidasjon ble registrert som en hurtig vektøkning i prøvene når deres oksidative motstand ble brutt ned, etter en initiell induksjonstid (IP). De respektive in-duksjonstidene for prøvene er vist i figur 3. The oxidative stability of the three oils was tested by exposing 3.0 g samples in 6.0 cm glass Petri dishes to a temperature of 35°C with exposure to air. The oxygen uptake caused by oxidation was recorded as a rapid weight gain in the samples when their oxidative resistance was broken down, after an initial induction time (IP). The respective induction times for the samples are shown in figure 3.
Claims (8)
Priority Applications (6)
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NO20023262A NO319618B1 (en) | 2002-07-05 | 2002-07-05 | Fish feed containing tocotrienol to improve the stability and pigment distribution in the fish fillet and processed fish products. |
EP03786448A EP1530428A2 (en) | 2002-07-05 | 2003-07-04 | Fish feed |
PCT/NO2003/000235 WO2004026040A2 (en) | 2002-07-05 | 2003-07-04 | Fish feed |
US10/520,149 US20060165840A1 (en) | 2002-07-05 | 2003-07-04 | Fish feed |
AU2003295287A AU2003295287A1 (en) | 2002-07-05 | 2003-07-04 | Fish feed |
NO20050591A NO20050591L (en) | 2002-07-05 | 2005-02-03 | Fish oil |
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NO20023262A NO319618B1 (en) | 2002-07-05 | 2002-07-05 | Fish feed containing tocotrienol to improve the stability and pigment distribution in the fish fillet and processed fish products. |
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US (1) | US20060165840A1 (en) |
EP (1) | EP1530428A2 (en) |
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JP3106820B2 (en) * | 1993-12-08 | 2000-11-06 | アールティーエー・アソシエーツ有限会社 | Livestock and fish meat and their processed products freshness preservative and productivity improver |
KR100226098B1 (en) * | 1995-03-06 | 1999-10-15 | 가타야마 시스카 | Processed fish meat, fish meat material prepared by using the same, and process for producing processed fish meat |
JPH099939A (en) * | 1995-06-29 | 1997-01-14 | Lion Corp | Food or feed containing natural antioxidant extracted from palm oil |
US6395915B1 (en) * | 1999-09-10 | 2002-05-28 | Technikrom, Inc. | Method for producing purified tocotrienols and tocopherols using liquid chromatography |
US6716451B1 (en) * | 1999-11-30 | 2004-04-06 | Soft Gel Technologies, Inc. | Formulation and delivery method to enhance antioxidant potency of vitamin E |
JP2004505645A (en) * | 2000-08-10 | 2004-02-26 | レネッセン リミテッド ライアビリティ カンパニー | Products containing corn oil and corn meal obtained from high oil corn |
US20020120001A1 (en) * | 2000-10-13 | 2002-08-29 | Ashni Naturaceuticals, Inc. | Compositions containing tryptamines, cartenoids and tocotrienols and having synergistic antioxidant effect |
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- 2003-07-04 WO PCT/NO2003/000235 patent/WO2004026040A2/en not_active Application Discontinuation
- 2003-07-04 US US10/520,149 patent/US20060165840A1/en not_active Abandoned
- 2003-07-04 EP EP03786448A patent/EP1530428A2/en not_active Withdrawn
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WO2004026040A3 (en) | 2004-05-13 |
WO2004026040A2 (en) | 2004-04-01 |
US20060165840A1 (en) | 2006-07-27 |
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