CN1430971A

CN1430971A - Method for lixiviating effective constituent of pilose antler in low temp. and ultrasonic cracking

Info

Publication number: CN1430971A
Application number: CN02100257A
Authority: CN
Inventors: 郑彬; 赵文秀; 于瑞
Original assignee: Individual
Current assignee: Individual
Priority date: 2002-01-11
Filing date: 2002-01-11
Publication date: 2003-07-23

Abstract

A process for extracting the active components from pilose antler features use of such techniques as low-temp extracting for high bioactive stability, ultrasonic cracking for extracting all active components, superfilter for interceping macromoleculae, and vacuum distillation for preventing deactivation of active components.

Description

Effective constituent of pilose antler low temperature ultrasonic degradation extraction

The present invention relates to the leach extraction method and the device of active ingredient in the Cornu Cervi Pantotrichum.

Cornu Cervi Pantotrichum is a kind of rare Chinese medicine, outstanding effect is being arranged aspect medicine, health care, the health preserving, its active ingredient is to be present in the bioactive substance that reaches between the Cornu Cervi Pantotrichum tissue in the cell, as superoxide dismutase, bioactive peptide, aminoacid and fatty acid etc., and these compositions general designation Cornu Cervi Pantotrichum elements.

The traditional process technology of Cornu Cervi Pantotrichum is stifling drying, and this method is subjected to restriction the scope of application of Cornu Cervi Pantotrichum, and the active ingredient effect of Cornu Cervi Pantotrichum can not brought into play comprehensively.Russia, Japan adopt n-butyl alcohol, ethanol etc. to extract medium, and complex production process has dissolvent residual and macromole extractum again, has influenced product quality.

Along with the continuous progress of biotechnology, the range of application of Cornu Cervi Pantotrichum bioactive substance is extensive day by day, and medicine, food, health product, cosmetic industry demand constantly increase.Cornu Cervi Pantotrichum is carried out polish, it is plain so that above-mentioned industry use is the market demand to extract Cornu Cervi Pantotrichum, it is again the direction of modern Chinese medicine development, improve the leaching rate of bioactive substance in the Cornu Cervi Pantotrichum, guaranteeing bioactive substance not oxidation inactivation in leaching process, is the key point that improves the plain quality and yield of Cornu Cervi Pantotrichum.

Thus, the present invention realizes above-mentioned purpose by following technical process:

1, whole extracting technology all carries out under 4-6 ℃ low temperature environment, and purpose is to guarantee the stability of bioactive substance in the Cornu Cervi Pantotrichum, not oxidation inactivation;

2, will add the weight ratio of extracting (50% alcoholic solution) Cornu Cervi Pantotrichum slurry in the medium and extracting medium through pulverize at low temperature, levigated Cornu Cervi Pantotrichum slurry is 1: 1.8, and low temperature stirs, and soaks 30-45 minute;

3, ultrasonic field is sent in the extracting solution circulation and carried out cracking, with the cracking of pilose antler cell film, the active substance in the cell is discharged, be dissolved in and extract in the medium, ultrasonic frequency is 28MHZ, and intensity is 100W, and the processing time is 90-120 minute;

4, ultra-filtration and separation is removed the solid material residue;

5, distilling under reduced pressure is reclaimed and is extracted medium, can obtain the plain thick product of Cornu Cervi Pantotrichum;

6, with thick separation and purification of products, can obtain the plain product of Cornu Cervi Pantotrichum.

The invention has the advantages that:

1, in low temperature environment, extracts bioactive substance in the Cornu Cervi Pantotrichum, can guarantee the biological activity of most materials.

2, ultrasonic degradation cracking pilose antler cell film effectively discharges the bioactive substance in the Cornu Cervi Pantotrichum fully, and is dissolved in and extracts in the medium, has improved the leaching rate of Cornu Cervi Pantotrichum element effectively.

Claims

A kind of low temperature ultrasonic degradation leach extraction method of effective constituent of pilose antler, its processing step is as follows:
(1) with Cornu Cervi Pantotrichum machinery chopping under 4-6 ℃ cryogenic conditions;
(2) the Cornu Cervi Pantotrichum particle is through the levigate slurry that causes 2-10um of colloid mill;
(3) the Cornu Cervi Pantotrichum slurry is added in the extraction medium (50% alcoholic solution), weight ratio is 1: 1.8, under 4-6 ℃ of cryogenic conditions, fully stirs lixiviate 30-45 minute;
(4) lixiviating solution is carried out at low temperatures the circulating ultrasonic cracking and handle, with the cell membranes in tissue cracking, ultrasonic frequency 28MHZ, ultrasound intensity 100W, processing time 90-120 minute;
(5) ultrafiltration and concentration separation removing raw material solid residue;
(6) distilling under reduced pressure is reclaimed and is extracted medium, obtains the thick product of Cornu Cervi Pantotrichum bioactive substance;
(7) thick product is carried out separation and purification, promptly obtain Cornu Cervi Pantotrichum active ingredient (Cornu Cervi Pantotrichum element) product.